Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,078,659	T→G	15.6%	H75P (CAC→CCC)	putA ←	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,078,659	0	T	G	15.6%	181.8 / 5.6	84	H75P (CAC→CCC)	putA	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (18/49);  new base G (14/2);  total (33/51)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTCCCATGCCATTACTCCTG  >  NC_000913/1078428‑1078895                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 aaTAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTc                                                                                                                                                                                                                                           <  1:324736/239‑1 (MQ=255)     aCCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CCAAGTCGAGGAATGGCTGGTGTGGTTC‑ctc                                                                                                                                                                                                                                       >  1:55338/1‑238 (MQ=255)          tGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGCTAGGCCCC‑GGTGATGCCGGCGCGGGAAACCGCCCGGGGGCATATTTGTGCGG‑CAAAATCGAGGAAGGGGCGGGGGGGGTC‑CTCGGCggg                                                                                                                                                                                                                                 >  2:324736/1‑239 (MQ=255)          tGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCCCGGGAAACCGACTGGGGCAATATTTGCCCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTc‑cctctccgg                                                                                                                                                                                                                                 >  1:234859/1‑231 (MQ=255)           ggACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGCAACCGCTTCGGTTTCCGGGCGGCGAAAGGCCCC‑GGGGATCGCGGCGCGGGAAACCGCCTGGGGGAAAATTTGCTCGG‑CACAGTCGAGGAATGGCGGGGGTGGGTG‑CTCTGCCggg                                                                                                                                                                                                                                >  2:359151/1‑238 (MQ=255)           ggACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGGGATCGCGGCGCGGGGAACCGACTGGGGGCATATTTGCTCGG‑CCAAGTCGAGGAATGGCTTGTGTGGtttcccgtccggg                                                                                                                                                                                                                                 >  1:744671/1‑228 (MQ=255)           ggACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGGTTCCGGGCGGCGATAGACCCC‑GGGGATCGCGGCGCGGGGAACCGCCTGGGGCAAAATTTGCTCGGCCAAAGCCG‑GGAATGGCTGGGGGGGGTT‑ctctcgcggg                                                                                                                                                                                                                                >  2:692897/1‑233 (MQ=255)                 tACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGCCTGGGGCAATAATTGCTCGG‑CAAAGTCGAGGAAGGGCGGGGGGGGGTc‑cctccccggggggggg                                                                                                                                                                                                                          >  2:845608/1‑224 (MQ=255)                 tACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGGGGGGTTC‑CTCTCCCGGGGTCg‑gg                                                                                                                                                                                                                         >  1:531024/1‑238 (MQ=255)                 tACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCCGGCTTGTTCCCGCAGCCTAGAAACCCCTTCGGTTTCCCGGCGGCGATAGGCCCCGGGGGTTGGCGGC‑CGGGAAACCGCCCGGGGGCAAATTTTTCCCG‑CAAAGTCGCGGGAGGGGCGGGGTGGtttccccccccgggggggg                                                                                                                                                                                                                           >  2:579899/1‑222 (MQ=255)                 tACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGAGGGCTTGTTCCAGCAGCATAGAAACCGCTTAGGGTTCCGGGCGGCGAGAGGCCGC‑GGGGATCCCGGCGCGGGGAACAGCCAGGGGCAAAATTTGTTCGG‑CAAAGCCGGGGAAGGGCGGGGGGGGGTC‑CCCTCCCGGGGTCg‑gg                                                                                                                                                                                                                         >  2:81386/1‑238 (MQ=255)                  aCCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAAGCCCC‑GGTGAGCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CCAAGTCGAGGCATGGCTGGGGGGGGTC‑CTCTGCCCGGGTTG‑GTg                                                                                                                                                                                                                        >  1:642960/1‑239 (MQ=255)                      gcgcGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGGTCGG‑CAAAGTCGAGGCATGGCTGGTGTGGTTC‑CTCTGCCGGGGACC‑GGGCTTc                                                                                                                                                                                                                    >  1:929847/1‑239 (MQ=255)                       cgcgACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGGGATAGGCCCC‑GGGGATCGCGGCGCGGGGAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGCGGAGAGGCTGGTGGGGTTG‑CTCTGCCGGAGGCG‑GTggtgca                                                                                                                                                                                                                   >  2:287396/1‑234 (MQ=255)                       cgcgACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CACAGTCGAGGAATGGCTGGGGTGGTTC‑CTCTGCCCGGGGCG‑GTGCtttc                                                                                                                                                                                                                   >  1:368759/1‑237 (MQ=255)                       cgcgACCACTGGCATTTTTTTGATTACGCAGTTTATCAGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGGTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGGTAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGGAAAATTTGTCCGG‑CACAGTCGGGGAATGGCGGGGGGGGGGT‑CTCTGcccgggggggggggggc                                                                                                                                                                                                                    >  2:929207/1‑224 (MQ=255)                          gACCACTGGCATTTTTTTGATTACGCAGTTGATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGAAGGCGGGTTTGTTCCAGCAGCATAGAAGCCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCg                                                                                                                                                                                                                <  1:81386/239‑1 (MQ=255)                          ccctcttGGCTTTTTTTTGATTTCGCGGTTTTGTGGACAGCGGGTACGCCAGGTTGGGCGCCTGTTCCGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCg                                                                                                                                                                                                                <  2:777991/233‑1 (MQ=255)                          cccccttGGTATTTTTTTGGTTTCGAATTTTTGTGGCCAGGTGGTACGCCGGTTGGGGGGGCAGGGAAGCACCCGGCGGCGGCAGGCGGGCTTGTGCCAGGAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACGGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCg                                                                                                                                                                                                                <  2:368759/233‑1 (MQ=255)                           aCCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGATGGGGAGGGTA‑CACTTCCGGGGGCG‑GGGCGTCAGCg                                                                                                                                                                                                                >  2:1092076/1‑238 (MQ=255)                            ccACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGCCTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGGGTGGGTC‑CTCTGCCGGGGTCG‑GTGCTTCATCGc                                                                                                                                                                                                               >  1:528794/1‑238 (MQ=255)                                       tttttGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGGGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCcg                                                                                                                                                                                                  <  1:692897/239‑1 (MQ=255)                                                              ccAGCGGATCCGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTGGTTCCAGCAGCATAGAACCCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCcg                                                                                                                                                                                                  <  1:387609/216‑1 (MQ=255)                                                                cgCTGATACGCCAGTTTGGGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAACCCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATAGTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTc                                                                                                                                                                         <  1:489130/238‑1 (MQ=255)                                                                  cTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGGGGAATGGCTGGTTTGGGTC‑CTCTGCCGGAGGCG‑GGGGCTCAACCGCC‑TCATTGGCCGCGCCCAAAAGAAGCGACGGTAGCCCCg                                                                                                                                                                       >  2:894550/1‑239 (MQ=255)                                                                  cTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGACAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCCCGG‑CAAAGTCGAGGAAGGGCTGGTTGGGTTC‑C‑CTTCTCGGGTCGCGGGCTTCATC‑CTCTTCCTTAGCCGCGCCAGCAagacgccccggaacccccg                                                                                                                                                                       >  2:444170/1‑222 (MQ=255)                                                                   tGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTgg                                                                                                                                                                                                                                              <  1:255965/170‑1 (MQ=255)                                                                    gATCCGCCAGTTTGGGCGGCTGTTCAGCAACGGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCgg                                                                                                                                                                      <  1:995751/238‑1 (MQ=255)                                                                      ttcggcAGTTTGGGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCa                                                                                                                                                                    <  1:203952/233‑1 (MQ=255)                                                                        cGCCAGTTTGCGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTTCAGCAGCATTGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGCCTGGGGCAATATTTGCTCGG‑CGAAGTCGAGGAAAGGCTGGGGGGGGTC‑CTCTGCCGGCGCCG‑GTGCTTCACCGCCC‑TCAATGGGCGGGCCAGAAagccggtctcataaaccaatctgaa                                                                                                                                                                 >  2:387609/1‑217 (MQ=255)                                                                           cagttaggccgacTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTAt                                                                                                                                                              <  1:1102291/227‑1 (MQ=255)                                                                             gTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCg                                                                                                                                                            <  1:565104/239‑1 (MQ=255)                                                                                   gcgcCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGGGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGttt                                                                                                                                                      >  1:131826/1‑239 (MQ=255)                                                                                                aaCTGGCTGCGGCAGGCGGGGTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTc                                                                                                                                           <  1:102640/237‑1 (MQ=255)                                                                                                 aCTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTTGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGGATCGCTGTTTTCCAGTTGTTCgag                                                                                                                                        >  1:901919/1‑239 (MQ=255)                                                                                                    ggCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATa                                                                                                                                     <  1:887525/239‑1 (MQ=255)                                                                                                                 gggTTTGTTCCAGCAGCATAGAACCCGTTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGc                                                                                                                         <  1:557725/238‑1 (MQ=255)                                                                                                                 gggCTTGGTCCAGCAGGATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGc                                                                                                                         <  1:359151/238‑1 (MQ=255)                                                                                                                    ttttatCCAGCAGCATAGAAACCGTCTCGTTTTCCCGGCGGCGAAAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTg                                                                                                                      <  1:837735/233‑1 (MQ=255)                                                                                                                    gtttgtcccggtgcaTTGAGACCGCTTAGGTGTCCGGGGGGCGCTAGGCCGC‑GGTGATGGCGGCGAGGGAAACTGACTGGGGCAATTTTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGTTC‑TCATTGGCCTCGCCAGAAAGCAGCGCAGGTAGCTCCTGCAGAGTATCGCTGTTTTCCAGTTGTTCGGGATAAGAAAAAATCGCCtt                                                                                                                      <  2:354621/226‑2 (MQ=255)                                                                                                                    gttGGTCCAGCAGCATGGAAGCCGGGTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGGGGGGAACCGAGTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTg                                                                                                                      <  2:744671/237‑1 (MQ=255)                                                                                                                    cTTGTTCCAGCAGCATGGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTg                                                                                                                      <  1:210651/238‑1 (MQ=255)                                                                                                                     agggcccggccggaTAGAAACCGTGTCGGTTTCCGGGGGGCGATGGGCCGC‑GGTGATCGCGGCGCGGGAACCCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCt                                                                                                                    <  2:1078830/226‑1 (MQ=255)                                                                                                                      tGTTCCAGCAGCATAGAAACCGTTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCt                                                                                                                    <  1:929207/238‑1 (MQ=255)                                                                                                                        gtCCAGCCGCATGGAAACCGCTTCGGTTCCCGGGGGGCGATAGGCCGG‑GGTTATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCttaa                                                                                                                 <  2:812763/238‑1 (MQ=255)                                                                                                                          cccgcagcaTAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGAGGGAAACCGGCTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCttaatt                                                                                                               <  1:601389/236‑1 (MQ=255)                                                                                                                          ccAGGAGGAAAGAAACCGGTTTGTTTTCCGGGGGGCGATAGGCGGG‑GGTGAGCGCGGGGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCttaatt                                                                                                               <  2:528794/239‑1 (MQ=255)                                                                                                                          ccAGCAGCAAGGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCttaatt                                                                                                               <  1:747385/239‑1 (MQ=255)                                                                                                                             gcagcaTAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GAGCTTCATCGCTC‑TCATTGGCCGGGCCCGAAAGCAGGCCAGGTAGCTCCGGCAGAGTATCCGTGTTTTCCCGTTGTTCGAGGTAAGAAAAAATCGCCTGCttaaataaa                                                                                                            >  2:198832/1‑238 (MQ=255)                                                                                                                               agcaTAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAAc                                                                                                            <  1:963842/237‑1 (MQ=255)                                                                                                                                 caTAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAgg                                                                                                        >  1:812763/1‑238 (MQ=255)                                                                                                                                  agaGAAGCCGCTTCGGTTTGCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGTGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAAcc                                                                                                           <  1:154285/233‑1 (MQ=255)                                                                                                                                        aCCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGGAGCTCCGGCAGAGGATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCa                                                                                                          >  1:1078830/1‑230 (MQ=255)                                                                                                                                            cTTCGGTTTCCGGGGGGCGATTGGCCCG‑GGGGATCGGGGGGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGcga                                                                                             <  2:131826/239‑1 (MQ=255)                                                                                                                                             ttCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGCAAGCAGCGACGGGAGCTCCGGCCGAGTATCCCTGTTTTCCAGTTGTGCCGGATAAGAAAAAATCACCTGCTTAATTAACCACGGTGGTGTGcggt                                                                                            >  2:306646/1‑237 (MQ=255)                                                                                                                                                             gcttgggCCGC‑GGGGAGCGGGGGGCGGGAAGACGGGTGCGTCAAAGTGTGGGCGG‑TAAAGTGGAGGAATGGCTGGTGTGTGTC‑CGCTGCGCGAGGCG‑GTGCTGCAACGCCT‑GCAATGGCCTCGCCAGAAAGCAGCGCAGGTAGCGCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            <  2:272331/233‑1 (MQ=255)                                                                                                                                                             cgATAGGCTGC‑GGGTACCGCGGGGCGGGAAGCCGATTGGGGCAATATTTGCTCGG‑CAAAGCCGAGGTATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTTGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            <  2:472163/238‑1 (MQ=255)                                                                                                                                                             cgATAGGCCGG‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            <  1:345623/238‑1 (MQ=255)                                                                                                                                                             cgATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            <  1:1032184/238‑1 (MQ=255)                                                                                                                                                              ggtgtggcgggggtggtgggggcggggGAAGCCGACGGGGCCAATATTTGGTTGG‑GAAAGTCGAGGCAGGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCGCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            <  2:234859/218‑1 (MQ=255)                                                                                                                                                                     cgc‑gGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCAGCGGTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTa                                                                    <  1:155640/238‑1 (MQ=255)                                                                                                                                                                       c‑gGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTTGCGCCGGCCGAAAGCAGGTCCGGTAGATCCGCCGGCGTAATCCGGTTTTCCCGTTGTTTGGGGTAAGCAAAAATAGCCGTCTTAATTAACCAGATTGGTGTGGCACCGATACG‑ATC‑GCGGGAGAcccttaaa                                                                >  2:171917/1‑232 (MQ=255)                                                                                                                                                                       c‑gGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTACTCGCTC‑TCATTGGCCGCGCCCGAAAGCAGCGCAGGTAGCTCCGGCCGCGGATCGCTGTTTTCCAGTTGTTTGAGATAAGAAAAAATCGCCCGCTTAATTAACCACGGGGGGGTGCGATCGAAAAGTGTCGGCGGC‑GACTTaaaa                                                                 >  2:1040139/1‑237 (MQ=255)                                                                                                                                                                                     gcggGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            >  1:372032/1‑215 (MQ=255)                                                                                                                                                                                     gcggGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCgg                                                                            <  2:372032/215‑1 (MQ=255)                                                                                                                                                                                        ggAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCAcgcgtcgcg                                                  <  1:306646/238‑1 (MQ=255)                                                                                                                                                                                            aCCGACTGGGGCAATTTTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCgtcgtc                                             <  1:501974/239‑1 (MQ=255)                                                                                                                                                                                            aCCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCgtcgtc                                             <  1:677103/239‑1 (MQ=255)                                                                                                                                                                                                   ggggCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGGGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGGTACGGGTC‑GCGGCCGACTTAATAAGCCCACGCGTCGCGTCGTCCAGCtt                                       >  1:554730/1‑238 (MQ=255)                                                                                                                                                                                                      gCAATTTTTGCCCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAAc                                    <  1:1092076/238‑1 (MQ=255)                                                                                                                                                                                                      gCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAAc                                    <  1:845608/238‑1 (MQ=255)                                                                                                                                                                                                      gCAATATTTGCCCGG‑CAAAGGCGAGGAATGGCTGGTGGGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAAc                                    <  1:894550/238‑1 (MQ=255)                                                                                                                                                                                                         atatTTGGTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAAcccc                                 <  1:444170/238‑1 (MQ=255)                                                                                                                                                                                                         atatTTGCTCGG‑CAAAGTCGAGGAATGCCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAAcccc                                 <  1:613180/238‑1 (MQ=255)                                                                                                                                                                                                          tttgtcttttg‑CAAAGGTGAGTATTGGGTTGTGTTGGTC‑CTCTGCCGGGGGCC‑GTGGTCCATCGCTG‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGCTGATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATAAGTGTC‑GCGGCAGACTTAATCCGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCa                               <  2:143020/229‑1 (MQ=255)                                                                                                                                                                                                                  tgg‑CAAAGTCGGGTAATGGCTGGGTTGTGTT‑CTCTGCCGGAGGCG‑GGGCTTCCACGGTC‑TCATTGGCCGGGCCAGAAAGCAGGGCAGGTAGCTCCGGCGGAGTATCGCTGTTTTCCAGTTGTTCGGGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCAtggtggtg                       <  2:554730/238‑1 (MQ=255)                                                                                                                                                                                                                    g‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCCCACCCGTCGGTCCGTCCAGCTTAACCCCCAtggtggggg                      >  1:158301/1‑238 (MQ=255)                                                                                                                                                                                                                        aaGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTcc                  <  1:287396/239‑1 (MQ=255)                                                                                                                                                                                                                           gcGAGGAATGGCTGGGGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTCCCa                <  1:178378/237‑1 (MQ=255)                                                                                                                                                                                                                            tggggAATGGTTGGT‑TGGTTCTCTCGTCCGGGGTGG‑GGGCTGCATGGCTT‑GCATTGGGCGCGCCAGAAAGCAGCGGAGGTAGCTCCGGCAGAGTATCGGTGGTTTCCAGTTGTTCGAGATAAGAAAAAAGCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTCCCAt               <  2:642960/235‑1 (MQ=255)                                                                                                                                                                                                                                  aTGGCTGGTGGTGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGGTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTCCCATGCCAt          <  1:782872/237‑1 (MQ=255)                                                                                                                                                                                                                                     gCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑CCGGCAGACTTAATACGCTCACGCGTCGCGTCGGCCAGCTTAACCCCCCTGTGGGGGGTTCCCATGCCATTAc       >  1:927320/1‑237 (MQ=255)                                                                                                                                                                                                                                     gCTGGCGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGAAGCGCAGGTAGCTCCGGCAAAGTATCGCTGTTTTCCCGTTGTTCGAGATAAGAAAAAATCGCCGGCTTAATTATCAAGTGTGGGGGGCGGTAGACACATGTA‑GAGGCCGACTTAAAACGCTCACGCCTGACGACGGCCAGGTTAACCCCCCCGGGGGTGGTcccccagcacttcaa      >  2:782872/1‑227 (MQ=255)                                                                                                                                                                                                                                        ggTGGGGTTC‑CTCTGGCGGGGTCG‑GGGCTTTCTCGCTC‑TCATTGGCCCCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTTTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTCCCATGCCATTACTCCtg  <  2:410413/239‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 AATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGC‑GGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGG‑CAAAGTCGAGGAATGGCTGGTGTGGTTC‑CTCTGCCGGAGTCG‑GTGCTTCATCGCTC‑TCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTC‑GCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCATGGTGGTGGTTCCCATGCCATTACTCCTG  >  NC_000913/1078428‑1078895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑