BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,415,383	T→G	16.2%	A668A (GCA→GCC)	recE ←	Rac prophage, exonuclease VIII, 5' to 3' specific dsDNA exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,415,383	0	T	G	16.2%	225.3 / 3.7	106	A668A (GCA→GCC)	recE	Rac prophage, exonuclease VIII, 5' to 3' specific dsDNA exonuclease
Reads supporting (aligned to +/- strand): ref base T (19/64); new base G (15/6); total (36/70)
Fisher's exact test for biased strand distribution p-value = 5.33e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGGACGGCACCGACACAAAATTCCTGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTGGCTCTATGTCTTCTACGATTACG  >  NC_000913/1415146‑1415620

cgggggggaCCGACAAAAAATTCCTTT‑TTCAGGAACTGCCCAGTAAATTGGAGATTCAGCGGGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTg                                                                                                                                                                                                                                                             <  2:427799/231‑1 (MQ=255)

cggacggaACCGACACAAAATTCCTGT‑TTCAGGTTCTTCCCAGTAAATTGATGATTCCGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGTCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTg                                                                                                                                                                                                                                                             <  1:23512/239‑1 (MQ=255)

cgcccgcccccgtcccaAATTTCCTGT‑TTCAGTATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTg                                                                                                                                                                                                                                                             <  2:295006/224‑1 (MQ=255)

cGGACGGCACCGACACAAAATTCCTGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGGGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTg                                                                                                                                                                                                                                                             <  1:77386/239‑1 (MQ=255)

 cgACGTCACCGCCACAAAGTTCCTGT‑TTCAGGATCTACCCAGTAAATTGATGATCCAGCGTGGCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTGCCGGACTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGc                                                                                                                                                                                                                                                            <  1:753105/238‑1 (MQ=255)

       cACCGACACAAAATTCCTGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TGTA‑TTTCAAGTGGTT‑CGAt                                                                                                                                                                                                                                                     >  1:858328/1‑239 (MQ=255)

        cccGGCACCAAATTCTCTT‑TTACGGGTCTACCCTGTAAATTGATGATCAAGGGTGTCCGGCGCTTTCAAAAAGCCATTGCCCAAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTG‑CGAt                                                                                                                                                                                                                                                     <  2:1020533/237‑1 (MQ=255)

            acacAAAATTCCTGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTTTACGGCGG‑TTAA‑AATTAGTGGCTT‑CGCTaaaac                                                                                                                                                                                                                                                >  2:323600/1‑237 (MQ=255)

               caAAATTCCTGT‑TTCATGAACGTCCCAGTTAATTGATGAGTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGt                                                                                                                                                                                                                                             <  1:730721/239‑1 (MQ=255)

                  aaatCCTGTGTTCATGCTCTTCCTAGAAAATGGAGTATACAGCGTGTCCGGGGCTTGCAAAAAGCCATTGCCCCAGCGGCAAAGCCAGAACTCTTGGATCAATGAGTTAAATTTCCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑Ta                                                                                                                                                                                                                                          <  2:854492/236‑1 (MQ=255)

                      ccTGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGATTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGaa                                                                                                                                                                                                                                     <  1:629952/239‑1 (MQ=255)

                        tGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCACTTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑GTAA‑ATTCCAGAGCTC‑CGGCAAAGCCGG‑TATTGAA‑t‑t                                                                                                                                                                                                                                 >  1:5485/1‑239 (MQ=255)

                        tGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TGTA‑ATTCTTGTGGTC‑CGCTAAAACgggttacggatt                                                                                                                                                                                                                                    >  2:509333/1‑229 (MQ=255)

                                   tCTTCCCAGTAAATTGTTTGTTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATTCATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTCTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTc                                                                                                                                                                                                                       <  2:210075/239‑1 (MQ=255)

                                   tCTTCCCAGTAAATTGATGATGCAGCGTGTCCGGCCCTTTAAACAAGCCATTGCCCCAGCCGCAAAGGCATAACGCTTTGATTCATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTc                                                                                                                                                                                                                       <  1:312253/239‑1 (MQ=255)

                                    cTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTc                                                                                                                                                                                                                       <  1:928415/238‑1 (MQ=255)

                                     ttCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑AATAAGGGGTTC‑CGCTAACCGGGT‑TACTGAA‑T‑TTTTTCGGGTC‑aa                                                                                                                                                                                                                    >  1:574615/1‑239 (MQ=255)

                                      tCCCAGTAAATTGTTGATTCAGCGGGTCCGGCGCTTTCAACAAGCCAATGCCCCGGCAGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTGCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTGCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAg                                                                                                                                                                                                                   <  1:592488/239‑1 (MQ=255)

                                       cccAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CTAGAAAACCGT‑TTCTGCA‑T‑TCTTTCCGTTT‑aaaa                                                                                                                                                                                                                  >  1:1060497/1‑237 (MQ=255)

                                          aGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTTAGGTGCTT‑CCATAAAACGGG‑TATTTAA‑A‑TCTTCCCGTTC‑acatat                                                                                                                                                                                                                >  1:400697/1‑233 (MQ=255)

                                            tAAATTGGTGATTCAGGCTTGCCGGCGCTTTCAAAAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTTATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑Ta                                                                                                                                                                                                                                          <  2:762779/213‑1 (MQ=255)

                                            tAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCG‑TTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTTTGGGGCTT‑CTATAAAACGGT‑GGTTGAA‑T‑TTTTCCgtgtcagttacaccc                                                                                                                                                                                                             >  2:322884/1‑225 (MQ=255)

                                             aaaTTGAAGATTCCGGTTGTCCGGGCTTTTCAACAAGGCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATCCATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTTATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCgg                                                                                                                                                                                                            <  2:606674/239‑1 (MQ=255)

                                              aatttgtgtttcaggtggtcgcgcgtTTACAACAAGACATTGCCCCAGCGGCAAAGCCATACCGCTTTGATACATGAGTTCAATTTTCCGGTCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATGCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCgg                                                                                                                                                                                                            <  2:841258/216‑1 (MQ=255)

                                              aaTTGATGATTCAGGGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCgg                                                                                                                                                                                                            <  1:784758/238‑1 (MQ=255)

                                               atttgtgttgccgCGTGTCCGGCGTTTTCAACAAGCCCTTGCCCCAGAGGCCACGCTATACCGCTTTGATACATGAGTTCCATTTTCCGGCCTTCTCCCGCAGTGATAACCGTGTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGT‑TTAA‑ATTCAGGGGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTCCCCGGCa                                                                                                                                                                                                          <  2:1079327/227‑1 (MQ=255)

                                                     gatTCAGCGTTTCCGGCGTTTTCAACCAGCCATTTCCCCAGTGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGaa                                                                                                                                                                                                   <  2:498546/239‑1 (MQ=255)

                                                      atTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCACGTGGTA‑CCGTAAATCGGT‑TACTGAA‑T‑TCTTCCTGttttctctacacatctcccag                                                                                                                                                                                                     >  1:762779/1‑219 (MQ=255)

                                                        tcggCGTGTCCGGCGTTTTCAAACAGCCCTTGCCCCAGCGGCAAAGCCATACCGCTTTGATACATGAGTTCAATTTGCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGc                                                                                                                                                                                                <  2:595953/236‑1 (MQ=255)

                                                        tcggCGGGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGc                                                                                                                                                                                                <  1:272991/236‑1 (MQ=255)

                                                        tcaggctgtCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGc                                                                                                                                                                                                <  1:249604/233‑1 (MQ=255)

                                                        tCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTTCGGGGGTA‑ACACAAAACGGG‑TACTGGA‑TGTTTCCCGGTTC‑ATAAACCCGGcggggaaaa                                                                                                                                                                                                   >  1:944454/1‑230 (MQ=255)

                                                         acggctgtCCGGTGTTTTAAAAAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCGTCCGCAGTTATAACCGTTTTTCCTGTGCTTGCGCAGTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCa                                                                                                                                                                                               <  2:1060497/234‑1 (MQ=255)

                                                          aGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTTAGGGGc                                                                                                                                                                                                                                                            >  1:168940/1‑183 (MQ=255)

                                                          aGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGc                                                                                                                                                                                                                                                            <  2:168940/183‑1 (MQ=255)

                                                          aGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTACGGGGGTA‑CGGTAAAACGGT‑TTATGAA‑T‑TCTTCCCGGTC‑TATTAACCCGCA‑GTGGgaaaaag                                                                                                                                                                                              >  1:672647/1‑235 (MQ=255)

                                                            cGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTTAGGGGCTA‑CGCTAAAACGGT‑TTCTGAA‑T‑TTTTTCGGTTT‑CAATACCCGCCC‑GTGGGAAACaat                                                                                                                                                                                             >  1:242383/1‑236 (MQ=255)

                                                                    gcgcTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑c                                                                                                                                                                                  <  1:323600/239‑1 (MQ=255)

                                                                    gcgcTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑AATTAAGTGGTT‑CGATAAAACGGG‑TACTGAA‑T‑TTTTCCGGTTC‑AAGTAACCCGCC‑GTTGAAAGACGTT‑TCTTAAt                                                                                                                                                                                    >  1:667145/1‑238 (MQ=255)

                                                                            aacaaGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑g                                                                                                                                                                             <  1:968631/234‑1 (MQ=255)

                                                                             gacaGCCAGTGCCCCTGGCGCAAAGTCATAGCGCTTTGATAGATGGGTTAATTTTTCCGGCCTTCTACCGAGGTTATAACCGTTTTTGCTGTGCTTGCGCATGCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTGCCGGTTC‑AAGTATCCGGCA‑GTGGAAATCAGTT‑CCTAAAT‑C‑GA‑GCGtttt                                                                                                                                                                       <  2:616/236‑1 (MQ=255)

                                                                             accaGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTTATAACCGGTTTTCCGTGGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGtttt                                                                                                                                                                       <  2:558004/236‑1 (MQ=255)

                                                                             acaaGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGtttt                                                                                                                                                                       <  1:1081127/239‑1 (MQ=255)

                                                                             aaaaGCCATTGCCCCAGGGGAAAAGCCATAACCGTTTGATACATGAGTTACATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGtttt                                                                                                                                                                       <  2:694808/237‑1 (MQ=255)

                                                                                    aTTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGATTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑AATCCAGTGCTT‑CCATAAAACCGT‑TATTGGA‑T‑ACTTCGGGTTT‑AATAACCACGCG‑TGGGAAAGCGGTT‑CCTAAATGA‑GA‑CCGtttttttttt                                                                                                                                                                 >  2:1006515/1‑234 (MQ=255)

                                                                                     ttGCCCCGGAGGCAAAGCCACAACGTTTTGATTCTTTTGTGTAATTTCTCGGCCTTTTTCCCCAGTGATCACCGTTTTACCTGTGCTTTCGCATGCGCTCAGAAACGCTTTCTCTTCTTCATTTCCGGCGTTTGTACGGCGG‑TTAA‑AGTCAGGTGCTA‑CGATAAAACGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑AA‑GCGTTTTTGTCTTtg                                                                                                                                                               <  2:72876/239‑1 (MQ=255)

                                                                                      tGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGGTTTAA‑ATTCTGGT‑CTA‑CGTAAAAACGGG‑TAATGAA‑A‑TCTTTCGGGTC‑CCGAACCCCGCC‑GTGGGAAAAAGTT‑TCCAAAA‑C‑AA‑GCGGTTTTTTTTTtgt                                                                                                                                                              >  2:968631/1‑239 (MQ=255)

                                                                                      tGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTTCGGGGCTC‑CGATAAAACGGT‑TTTTGAATT‑TTTTCGGGTTC‑ATATACCGGCGA‑GGGGAAA‑CGGAT‑CCTAAAA‑T‑GC‑GCGGTTTTTTCttttt                                                                                                                                                              >  1:806352/1‑237 (MQ=255)

                                                                                      tGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGGGCTA‑CGATAAAACGGG‑TACTTCA‑T‑TCTTCCGGGTC‑AAATACCCGGCC‑GTGGGAAGCAGTT‑CTCAAAT‑A‑GA‑GCGTTTTTGTCTTtgt                                                                                                                                                              >  1:46006/1‑239 (MQ=255)

                                                                                            aGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTCAGCTGCTG‑AGGTAAAACGGG‑TTCTGTA‑T‑TTTTCCGGTTT‑AATTAACCCGCC‑GTGGAAAACCATT‑CCCTAAT‑C‑CA‑GGGTttttttttttttttgtt                                                                                                                                                         >  1:1094210/1‑231 (MQ=255)

                                                                                            aGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAAGGGCTA‑CGATAAAACGGT‑TTCTGAA‑T‑TCTTCCGGTTC‑ACGTACCCGGCA‑GGGGGAAGAAGGT‑CCCAAAT‑A‑GAGGGGTTTTTTTCttttggggt                                                                                                                                                          >  1:720830/1‑232 (MQ=255)

                                                                                             gCGGCAAAGCCATAACGCTTTGATACATGAGTTCAA‑TTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGGGCTT‑CCATAAAACGGG‑TAATCAA‑T‑TCTTTCTGGTC‑CAGAACCCCGCA‑GCGGACAGAAGGT‑CCTTAAT‑C‑TC‑AGGTTTTTTTCTTTGTTG‑T‑GT‑c‑c                                                                                                                                                   >  1:599733/1‑238 (MQ=255)

                                                                                                  aaaGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACACCTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTCA‑AATCAACGGCTA‑CCTCAAAGCCGTGTTCTG‑A‑T‑TCTTTCGGTTG‑CAGAAACCCGCACGGGGAAGACAGCT‑‑CTAAAT‑A‑AG‑GCGGTTTTGTTTTTTTGG‑T‑GT‑G‑Cagcgg                                                                                                                                              >  2:855200/1‑235 (MQ=255)

                                                                                                        aTAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTTAAGTGGTACCTTTAAAGGGGT‑TATTGAA‑T‑TTTTCCGTTTC‑AAGTACCCGCCC‑GGGCAAAGAATTC‑CCTAAAT‑C‑GA‑CGGTTTTTGT‑TTTTTGG‑G‑GT‑C‑CCCCGGGGGAtt                                                                                                                                        >  2:649498/1‑239 (MQ=255)

                                                                                                             gttttGAATCATGTGTTCAATTTTCCGACCTTTTTCCGCCGTGTTAACCGTTTTTCCTGTGCGTGCTCATTCCAACAGAAACGCTTTCTCTTCGTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑AGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTAc                                                                                                                                   <  2:986288/237‑1 (MQ=255)

                                                                                                             gcttttgttcATGATTTCAATTTTCCTGCCTTCTTCCGCCGTGTTAACCGTTTTTCCGGTTCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTTAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTAc                                                                                                                                   <  2:667145/230‑1 (MQ=255)

                                                                                                             gcttttatcatttgtttCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGGGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTAc                                                                                                                                   <  2:963285/224‑1 (MQ=255)

                                                                                                             gcttgtgagactGAATTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTAc                                                                                                                                   <  1:855200/228‑1 (MQ=255)

                                                                                                             gCTTTGATGCATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCGTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTAc                                                                                                                                   <  1:700196/239‑1 (MQ=255)

                                                                                                               ttttttACTTGATTTAAATTTTCCGGCCTTCGTCCGCGTTGATAACCGTTTTTCCGGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGc                                                                                                                                 <  2:247980/234‑1 (MQ=255)

                                                                                                               ttttctacttggtttCATTTTTCCGGACTTTTTCCGCAGTGATAACCGTTTGTCCTGGGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTGCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGc                                                                                                                                 <  2:493919/226‑1 (MQ=255)

                                                                                                               tttgtttcttgtttttatttttCCGGCCTTCTTCCCTTGTGATTACCGTTTTTCCTGTCCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGc                                                                                                                                 <  2:495682/221‑1 (MQ=255)

                                                                                                               tttGTTACATTATTTTAATTTTCCGGCACTCTTCCGCAGTTATACCCGTTTTTCCTGTGCTTGCTCATTCCAGCAGAAACGCTTTCTCTTCTTCATTTCCGGCGTTTGTACGGCGG‑ATAA‑ATTCAGGTGCTA‑CGATAAAGGGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCACTTTTACGc                                                                                                                                 <  2:885838/239‑1 (MQ=255)

                                                                                                               tttGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCTTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGc                                                                                                                                 <  1:573459/239‑1 (MQ=255)

                                                                                                               tttGAGACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGc                                                                                                                                 <  1:144212/239‑1 (MQ=255)

                                                                                                                ttGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTTAAGGGCTA‑CGATAAAACGGG‑TCTTGAC‑T‑TCTTTCGGGTC‑CAGTACCCCGCC‑GTGGAAAACAGTT‑TCTAAAT‑C‑TG‑GAGTTTTTTGCTTTTTGG‑G‑GT‑T‑CCCCGGGGGATTTTTTCGc                                                                                                                                 >  1:790904/1‑238 (MQ=255)

                                                                                                                ttGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCTGGTGCTA‑CGATAAAGAGGG‑TTCTGTA‑A‑TCTTCCCGTTT‑ATATACCCCCCC‑CGGGAAAACAGTG‑CCTCAAT‑A‑GA‑GCGGGTTTTTCTTTTTTG‑TGGC‑C‑CACGGGGGAGTTTTAACg                                                                                                                                  >  2:516436/1‑238 (MQ=255)

                                                                                                                ttGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑CTAATATTCCGCTGC‑A‑CGGTAAAACAGG‑TATTGAA‑G‑TCTTCCGGTTC‑ACGTATCCCGCC‑GCGGGAAACCGTT‑TCCCAAT‑A‑CC‑GAGTTTTTTTCTTTTTGG‑G‑GG‑C‑CACgggggggtgtttttacc                                                                                                                                >  2:824686/1‑227 (MQ=255)

                                                                                                                ttGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑CTAA‑ATTCAGATGCTT‑CGATAAAACGGG‑TACTGAA‑T‑TCTTCTGGGTCAAAGAACCCGCCA‑‑CGGAAAGCAATT‑TCTAAAA‑C‑GA‑GCGGTTTTGTCTTTGTGG‑G‑GTGC‑AACGGGGGGAttttaagcc                                                                                                                                 >  2:452717/1‑234 (MQ=255)

                                                                                                                 tGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTCTGGTGCTA‑CGATAAAACGGT‑TTCTGAA‑T‑TTTTCCGCTTC‑ACTAACCCCGCC‑GTGGAAAGCAGTT‑CCCAAAT‑C‑CT‑GCGGTTTTTTTTTTTTGG‑T‑GT‑G‑CACGGGGGGCATTTTAAGCca                                                                                                                               >  2:573459/1‑239 (MQ=255)

                                                                                                                    tACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑Ca                                                                                                                                                  <  1:619322/217‑1 (MQ=255)

                                                                                                                       aTGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTTCAGTGCTA‑CGATAAAACGGG‑TACTGAA‑T‑TCTTTCGGTTC‑AAGGACCCGGCA‑GTGGTAAGCAGTT‑CCTAAAT‑C‑GG‑GCGGTTTTTTCTTTGTCG‑T‑GT‑C‑CACGGGGGCACTTTTTAGCCACAAata                                                                                                                         >  1:892764/1‑239 (MQ=255)

                                                                                                                       aTGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCCGGGGCTT‑CGGTAAAACGGG‑TTATGGA‑T‑TCTTCCGGTTT‑ACATAACCGGCC‑AGGGAAAACAGGT‑CCTTAAT‑CGGA‑GGGTTTTT‑TCTTTTTGG‑G‑GT‑CTCACGGGGCAAttttttgcccccacaa                                                                                                                          >  1:693138/1‑228 (MQ=255)

                                                                                                                       aTGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCCGGGGCTA‑CGATAAAACGGG‑TACTGAA‑T‑TTTTCCGGTTC‑AAGTACCCGGCA‑GTGGGAAACAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTTTTTTTTTGG‑T‑GT‑C‑CCCGGGGGCCttttttaccccccaaaa                                                                                                                         >  1:963285/1‑227 (MQ=255)

                                                                                                                              tcaTTCTCCGGCCTTCTTCCCGAGTGATAACCTTTTTTCCTGTGCTTGCGCATTCCAACAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCTGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGACAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGcc                                                                                                                  <  2:695400/237‑1 (MQ=255)

                                                                                                                              cAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTTCGGTGCTA‑CGGTGAATTGGG‑TATTTGA‑T‑TCTTTCGGTTC‑CAATAACCGGGG‑AGGGCAAGAAGTTATCTAAA‑‑C‑AA‑CGGTTTTTGTCTTTTTGGTT‑TT‑C‑GACGGGGGGATTTTTTACCCACaaaaaaatgtc                                                                                                                   >  2:652530/1‑231 (MQ=255)

                                                                                                                               aaTTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTTCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCg                                                                                                                 <  1:287942/239‑1 (MQ=255)

                                                                                                                                   ttCCGGCTTTTTCTCGCAGTGATACCCTTTTTTCCTGCGCTTTCGCATTCCATCAGAATCGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCTG‑TTAA‑CTTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGt                                                                                                             <  2:300460/239‑1 (MQ=255)

                                                                                                                                      cGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTTA‑ATTCTAGTGCTA‑CGATAAAACGGG‑TATTTAA‑T‑TCTTCCGGGTC‑AAAGACCCCGCA‑GTGGGAAACAGTT‑TCTAAAA‑C‑GA‑GCGGTTTTGTGTTTTTGG‑T‑GT‑C‑CCCGGGGGCCTTTTTTCGCCACACATATAGTGCCGGAGTATc                                                                                                          >  1:490713/1‑239 (MQ=255)

                                                                                                                                                 cctggTGATAACCTTTTTTCTTGTGCTTGCGCATTCCCACATAAACGCTTTCTCTTCTTCTTTTCCAGTGTTTGTACGGCGG‑TTAA‑ATTCCGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCAt                                                                                               <  2:1094210/235‑1 (MQ=255)

                                                                                                                                                 cGCTGTGTTAACCGTTTTTCCTGTGCTTGCGCATTCCCTCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCAt                                                                                               <  2:672647/239‑1 (MQ=255)

                                                                                                                                                        aTAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGGTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCt                                                                                         <  1:81867/238‑1 (MQ=255)

                                                                                                                                                         tAACCGTTTTTCCTGTGGTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTga                                                                                       <  1:14320/239‑1 (MQ=255)

                                                                                                                                                                  tttctttgtttGCTCATTCCATTAAAAAGCCTTTCTCTTTTTCTTTTCCGGCGTTTGTAGGGCGG‑TTAA‑ATTCAGGTGCTC‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCGGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑Tg                                                                             <  2:599733/230‑1 (MQ=255)

                                                                                                                                                                  ttCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑Tg                                                                             <  1:167504/239‑1 (MQ=255)

                                                                                                                                                                            ttccttttccctccaaaaccgttttTCTTCTTTTTTTCCGGCGTTTGTTCGGCGG‑TTAA‑ATTCAGGGGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGAc                                                                   <  2:648023/218‑1 (MQ=255)

                                                                                                                                                                                                cTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTTAGGTGCTA‑CGATAAAAAGGT‑TACTTAA‑T‑TTTTCCGGTTC‑AAGTACCCCGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGGTTTTGTCTTTGTGG‑T‑GT‑C‑CCCGGGGGCATTTTTACGCCCCAAAAATAAGGCCGGAGGATTAGCAATGTCATCGGGCTGTGAGTTACTTGAAACCGGGGCCC‑CGGGGGAATTCTCATTc                                               >  1:43318/1‑239 (MQ=255)

                                                                                                                                                                                                    cgcttcttcttTTCCGGGGTTTGTACGGCGT‑GTAA‑ATTCAGGTGCGA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGaaa                                           <  1:1061082/237‑1 (MQ=255)

                                                                                                                                                                                                    cccttcttcttTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGaaa                                           <  1:1043486/237‑1 (MQ=255)

                                                                                                                                                                                                         ctgcgttgccGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACGGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCg                                      <  1:756011/231‑1 (MQ=255)

                                                                                                                                                                                                               ttCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGGGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGtaataa                                <  2:806352/239‑1 (MQ=255)

                                                                                                                                                                                                                   ggCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACGGAA‑T‑TCTTCCGGTTC‑AAGTTCCGGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATAc                            <  2:791616/239‑1 (MQ=255)

                                                                                                                                                                                                                    gCGTTTGTACGTCTG‑TTAA‑ATTCAGGTTCTA‑CGGTAAAGCCGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAG‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATAcc                           <  2:957135/239‑1 (MQ=255)

                                                                                                                                                                                                                     gtttttgtgggcgg‑TTAA‑AATCAGGGGACA‑CGGTAAAGCGTT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGGCGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGGCTTTGTGG‑T‑GT‑C‑CACGGGAGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCGCATTCGAAATTCCGTAATAAATAcc                           <  2:892764/229‑1 (MQ=255)

                                                                                                                                                                                                                        ttGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTgg                        <  1:558241/238‑1 (MQ=255)

                                                                                                                                                                                                                           tgcggcgg‑TTAA‑ATTCAGGGGCGA‑CGGTAAACGGGT‑TTCTGAA‑T‑TCTTCCGGTTC‑AAGGACCCGGCA‑GGGGAAAGCAGTT‑CCTAAAG‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTGGctct                    <  2:1007068/237‑1 (MQ=255)

                                                                                                                                                                                                                                      aa‑aTTCAGGTTCTA‑CGATAAAGCGGT‑TCCTGAA‑T‑TCTTCCGGTTC‑AAGTTCCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTGGCTCTATGTCTTCTa          <  2:319703/239‑1 (MQ=255)

                                                                                                                                                                                                                                      aa‑aTTCAGGGGTTT‑CGTTAAAGCGGT‑TTCTGAA‑T‑TGTTCCTGTTC‑AAGTGCCCGCCA‑GTGGAAAGCAGTT‑CCTAAAG‑C‑GA‑TCTTTTTTGTCTTTGTGG‑G‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTGGCTCTATGTCTTCTa          <  2:709259/239‑1 (MQ=255)

                                                                                                                                                                                                                                               gTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTACAT‑C‑GA‑GCGTTTTTGTCTTCGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCCCAAATATAGTTCCGGTGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCCTGGTAATTTTCCTTCTAAATTCCGTAATAAATAccggcgccattagtttctaacatt     >  2:40774/1‑214 (MQ=255)

                                                                                                                                                                                                                                                tGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCCTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGGCTGTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTGGCTCTATGTCTTCTACGATTACg  <  1:595936/238‑1 (MQ=255)

CGGACGGCACCGACACAAAATTCCTGT‑TTCAGGATCTTCCCAGTAAATTGATGATTCAGCGTGTCCGGCGCTTTCAACAAGCCATTGCCCCAGCGGCAAAGCCATAACGCTTTGATACATGAGTTCAATTTTCCGGCCTTCTTCCGCAGTGATAACCGTTTTTCCTGTGCTTGCGCATTCCATCAGAAACGCTTTCTCTTCTTCTTTTCCGGCGTTTGTACGGCGG‑TTAA‑ATTCAGGTGCTA‑CGATAAAGCGGT‑TACTGAA‑T‑TCTTCCGGTTC‑AAGTACCCGGCA‑GTGGAAAGCAGTT‑CCTAAAT‑C‑GA‑GCGTTTTTGTCTTTGTGG‑T‑GT‑C‑CACGGGGGCATTTTTACGCCACAAATATAGTGCCGGAGTATCAGCAATGTCATCGAGCTGAGACTTAC‑TGATACCGGGACCCGCGTGGTAATTCTCATTCGAAATTCCGTAATAAATACCTGGCTCTATGTCTTCTACGATTACG  >  NC_000913/1415146‑1415620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`