Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,435,704 G→A 12.1% intergenic (‑85/+56) uspF ← / ← ompN stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,435,7040GA12.1% 193.4 / 3.2 75intergenic (‑85/+56)uspF/ompNstress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (54/9);  new base A (3/7);  total (59/16)
Fisher's exact test for biased strand distribution p-value = 5.73e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACA  >  NC_000913/1435467‑1435940
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
aCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGa                                                                                                                                                                                                                                               >  1:473012/1‑239 (MQ=255)
                  gggCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATTACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTAAATTTAGATAAAAGGGGGAAGAaaaa                                                                                                                                                                                                                           >  2:399098/1‑239 (MQ=255)
                    gCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCACAGGAGGAAGAATAAGc                                                                                                                                                                                                                         >  1:1024639/1‑239 (MQ=255)
                     cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAAAGAGGCTAATCACGCGTTGAGTTAATTCTGAATCGGCAATATCGAAAGGGACAAGAAACGGTCTGTTCATAAAAACTCCTGTATTAGTATCCGCATAAAGTGAAACGCCAGAGGAAACTTTTTTGGA‑‑AAAGACGGGGTAAAAATGTTTAATTTAAAAAAAAGGAGGAAGAAAAAACa                                                                                                                                                                                                                        >  2:604244/1‑239 (MQ=255)
                       gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGTAAATATCGATAGGGACAAGAACCGTTCTGTTCATAAAACCTCCTGTTTTAGAATCCGCATAAAGTTAAACGCCCGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGAAGGAAAAATAAGCaaa                                                                                                                                                                                                                      >  2:532745/1‑237 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCCTAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTACAGCCCGATTACACTTTTTTTGT‑‑AAGACGGGAGTTCACATTTTTAATTTAAGACAAAGGAGGAAAAATAAGcgaaa                                                                                                                                                                                                                     >  2:979297/1‑235 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAgaagaa                                                                                                                                                                                                                     >  1:1093464/1‑239 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGGGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGTAAGAATAAgacgaa                                                                                                                                                                                                                     >  1:555980/1‑234 (MQ=255)
                          aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGGGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaaa                                                                                                                                                                                                                   >  1:104927/1‑239 (MQ=255)
                             gTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTTTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAg                                                                                                                                                                                                                 >  1:471070/1‑238 (MQ=255)
                                 tACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGc                                                                                                                                                                                                            >  1:219085/1‑239 (MQ=255)
                                      tCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGCAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGTTCAATGGAGGAAGAATAAGCAGAAAAACCCCCCCATa                                                                                                                                                                                                        >  2:623170/1‑238 (MQ=255)
                                        aGGAAATGCACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGCTGAGTTAATTCTGAATCGGAAATATCGACAGGTACACGCATCGCTCTGTTCATAAAACCTCCGGTTTTAGAATCCCCAAAAAGTCTAACGCCAGCTGGCACTTTTTGTGT‑‑AATAACGGAGTTCACATTTTTAATTTAAACCCAAGGCGGCAACATAAACAACAAAACCCCCCAATaaaa                                                                                                                                                                                                     >  2:789356/1‑237 (MQ=255)
                                        aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAATTATCGATAGGGACAAGAGTCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTTTGT‑‑AATGACGGCGTTCCCATTTTTAATTTATATCAAAGGAGGAAGAATAACAAAAAAAAGCCCCCCAAAACa                                                                                                                                                                                                     >  2:412392/1‑239 (MQ=255)
                                        aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGCGGAAGAATAAGCAGAAAAAGCCCCCCATaaa                                                                                                                                                                                                      >  1:625926/1‑237 (MQ=255)
                                        aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACa                                                                                                                                                                                                     >  1:552642/1‑239 (MQ=255)
                                                        catcatCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTAACATTTTTAATTTAGATCAATGAAGGAAGAATAAGCAGAAAAAGACACCCAAAAAACCGGGCagagagattt                                                                                                                                                                                     >  2:97201/1‑231 (MQ=255)
                                                             tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGAGAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGCTGACACTTTTTGTGT‑‑ACTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGAAGGAGGAGTTAGAAc                                                                                                                                                                                >  1:1037971/1‑239 (MQ=255)
                                                             tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAACAGAAAAAGCCCGCCCTAACACCGGGCAGGAGGATTTAGAAc                                                                                                                                                                                >  2:1033537/1‑239 (MQ=255)
                                                                     cgCCTCTCCCTCAACGGGGAAAATCACGGGTTGTATTAATTCTGAACCGAAATGTGCGAAAGGAACAAGAATCGTTCTGTTAAAAAACCCCCCTGTTTGAGTACCCGATAAAAGTGAACCGCAAGAGGACCTTTTTTGTTA‑‑AATGAGGGAGTTCACATTTTAAATTAAGATCAAGGGAGGAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                                                                                                                                        <  1:623170/238‑1 (MQ=255)
                                                                      gCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGCGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATATGCAGAAAAAACCCGCCATAACAGCGGGCAGGAGGGTTTAAAAACGAAAAAca                                                                                                                                                                       >  2:478216/1‑238 (MQ=255)
                                                                       ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAAAGCGGGCAGGAGGATTTAGAAATGAAAAAcaa                                                                                                                                                                      >  2:180703/1‑237 (MQ=255)
                                                                           ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCCTAACAGCGGGCAGGAGGATTTAGAACTGATaaaccaaacc                                                                                                                                                                  >  1:432727/1‑239 (MQ=255)
                                                                                 aaCGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCCGAAAAAGCCCGCCATAAAAGTGGGCAGGGGGGTTTAGAACTGAAAAACCAgcaccaaaac                                                                                                                                                            >  2:710805/1‑230 (MQ=255)
                                                                                     tGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGCGTAACGCCAGATGACACTTTTTTTGT‑‑AAGGACGGAGTTAACATTTTTAATTTAGATCAAag                                                                                                                                                                                                                                        >  2:319405/1‑160 (MQ=255)
                                                                                       gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      >  1:253979/1‑239 (MQ=255)
                                                                                        ttaatcctggtttGATTTATTTCTCAACGAGAATTAGCGATAGGGAAAAAAATCGTTTCTTCAAAAAACCCTCTTTTTTTATTTACCGAAAAAAGTGAAACGCCAGATGACTTTTTTGTTTAAAGGACGGATTTATCATTTTTAATTAAGAAAAAGGGAGAAAAAATAAGCAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  2:859144/227‑1 (MQ=255)
                                                                                        taaagcacgatgtGATTTAATTGTGAAGCGAAAATACGGATAGGGAAAAAAAGGTTTGGTTACATAAAACCCCCTTTTTAATTATCCGCATAAAGTTAAACGCGAGATGGACTTTTTGGGGA‑‑AATGACGGAGTCAACATTTTAAATTAAGACCAAGGGAGAAAGAAGAAGAAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTGAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  1:789356/226‑1 (MQ=255)
                                                                                         taaaactcgtttGTGTTTATTTTTAAGCGGAAATTTCGAAAGGGAAAAGAGTTTTTTTGTTAAAAAACCCTCTTTTTTTTGTCTACCCAAAAATGTATAAGCCAAAGAGACCTTTTGGGTAAAAGGGGGGAGTCCACTTTTTAAATTTAGAAAAAGGGGAAAAGAAAAAGAAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  2:601421/228‑1 (MQ=255)
                                                                                                 cgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACATCGGCAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                                                                                                                            >  1:606007/1‑239 (MQ=255)
                                                                                                 cgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                                                                                                                            >  1:882729/1‑239 (MQ=255)
                                                                                                    tGATTTATTTCTAAAGCGAAAATATGAATAGGCAAAAAAATCTTTCTGTTAATAAACCCCCCTTTTTAAGTATCCAAAAAAAGTGAACCGCAAGATGACTCTTTTTGTGT‑‑AATGACGGAGTTCATATTTTTAATTTAAAAAAAAGGAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTagag                                                                                                                                         <  1:180703/239‑1 (MQ=255)
                                                                                                                gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCgg                                                                                                                                              >  1:338971/1‑222 (MQ=255)
                                                                                                                gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCa                                                                                                                             >  1:714493/1‑239 (MQ=255)
                                                                                                                gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAACTAAAGCGAAAATAACACAGGTCGAGATGACAATggaa                                                                                                                             >  2:238290/1‑237 (MQ=255)
                                                                                                                 aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                                                                                                                            >  1:256476/1‑239 (MQ=255)
                                                                                                                       aaaTATGGAGGGGAAAAAAAATTGTTCTTTAAAAAAACCCTCTTTTTTTAGTTCCCAAAAAAAGTTAACCGCAAGATGAATTTTTTGGGTAAAGGAGGGAGTTCACATTTTAAATTAAGAAAAAAGGAGGAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg                                                                                                                       <  1:604244/238‑1 (MQ=255)
                                                                                                                         atgtgtaaaagagACAGGATTTGTTGTTTTCATAAACCCTCCTGTTTTAGTACCCGAATAAAGTGAAACGCCAGATGACTTTTTTTGTAT‑‑AATGACGGAGTTCACATTTTTAATTAAAATAAAGGGAGAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                                                                                      <  1:215378/226‑1 (MQ=255)
                                                                                                                              cGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAAATGCCATTGGCAGCGTAGAAGcagc                                                                                                                >  1:463793/1‑236 (MQ=255)
                                                                                                                                          gAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCAACAATACCAACGGGAAAGAAGCCATTGGCAGCGAAAAAACGGTCAACttacacca                                                                                                    >  2:885005/1‑233 (MQ=255)
                                                                                                                                              cGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAACGAAAAAACTGTAAAATTCATCCAAAAg                                                                                                >  2:374573/1‑239 (MQ=255)
                                                                                                                                                ttCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGc                                                                                                                                                                                               >  1:930785/1‑142 (MQ=255)
                                                                                                                                                ttCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt                                                                                              >  1:539514/1‑239 (MQ=255)
                                                                                                                                                ttCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGGGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGCAGCATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTATAAGCTGTCATCTTCATCCAACAGGt                                                                                              >  1:711003/1‑239 (MQ=255)
                                                                                                                                                  cTGTTCATAAAACCTTCTGTTTGAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGCGTTCACATTTTTAATTTATATCAAAGGAGGAAGAATAAGCAGACAAAGCCCGCAATAACAGCGGGAAGGAGGATTTCGACCTGATAAACAAGAACTAAAGCGAAACTATCATCGGTAGAGATGCCATTGGCAACTAAAAAGGAGTCATCTTCAACCAAAAGGTTg                                                                                            >  2:794111/1‑239 (MQ=255)
                                                                                                                                                     ttCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAACGGCAATATCATCGGTAGAGATGCCATTGGCAGCGAAAAAGCTGTCacccttgtcccttaaaaaaaac                                                                                         >  2:215378/1‑218 (MQ=255)
                                                                                                                                                              aCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc                                                                                 >  1:678399/1‑239 (MQ=255)
                                                                                                                                                                    ctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCAGTCATCTCAATCCAACAGGTGGATTTTATAGTCAACga                                                                            >  1:232079/1‑238 (MQ=255)
                                                                                                                                                                           attccccaaaaatagtagaaCGCCAGATGCCTTTTTGTGGTT‑‑AATCGCGGGGTTCAAATTTTTAATTAAAATCAAGGGAGAAATAAAAAGAAGAAAATGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGa                                                                     <  1:363697/221‑1 (MQ=255)
                                                                                                                                                                             tATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAATCTGTCATCTTCATCCAACAGGTTGATTTTATATCAAAAGAAGGTGGaaa                                                                   >  2:518043/1‑237 (MQ=255)
                                                                                                                                                                              aTCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGAAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGTTTTTATAGTCAACGTAGGTGGACAt                                                                  >  1:986202/1‑239 (MQ=255)
                                                                                                                                                                                 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa                                                                                                                                                                                 >  1:726683/1‑125 (MQ=255)
                                                                                                                                                                                 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGCCATGtt                                                               >  2:869/1‑239 (MQ=255)
                                                                                                                                                                                 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                               >  1:760898/1‑239 (MQ=255)
                                                                                                                                                                                 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAGGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGACAACGAAGggggcaaagtt                                                               >  2:742410/1‑232 (MQ=255)
                                                                                                                                                                                           gtAACGCCAGCTGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGTGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACCGATAAACCAGACCTAAAGCGACAATATCATCGGTATGGATGCCATTGGCACGATAGAAACTGTCATCTTCCTCCAAAAGGCTGATTTTATAGTCAACGAAGGGGGACATGTTTTTTATGaaa                                                     >  2:989249/1‑239 (MQ=255)
                                                                                                                                                                                             gtaacccaaaatgcacTTTTTGGTGA‑‑AATGACGGATTTCACTTTTTTAATTTAGAAAAAGGGAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGaa                                                      <  1:885005/225‑1 (MQ=255)
                                                                                                                                                                                             aaCGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATa                                                   >  2:332021/1‑239 (MQ=255)
                                                                                                                                                                                               aCGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                  >  1:980520/1‑239 (MQ=255)
                                                                                                                                                                                               aCGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                  >  1:412979/1‑239 (MQ=255)
                                                                                                                                                                                                     gATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa                                                                                                                     >  1:428107/1‑166 (MQ=255)
                                                                                                                                                                                                          ttgtttttGTGA‑‑AATGACGTAGTTCAATTTTTTAATTAAAAACAAAGGAGAAAAAATAAGCAAAAAAACTCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                        <  1:264654/235‑1 (MQ=255)
                                                                                                                                                                                                           acTTTTTGTGT‑‑AATTACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGAAGGAGTTAGAAGTGATAAACCAAGCCGAAAGCGACAATATCATCGGTAGAGATGCCATTGGGACCGTAGAAGCTGTCATCTTGATCCAACAGTTTAATTTTATCGTCAACGTAGGAGGCCATGTTTTTATTTAAAAAAAAAACCGCGCCa                                      >  2:648930/1‑239 (MQ=255)
                                                                                                                                                                                                                   ttttttgtatggacggtttatattttttAAATTAAAAAAAAGGGAGAAAAAAAAAACAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAata                                   <  2:760898/216‑1 (MQ=255)
                                                                                                                                                                                                                      t‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGt                            >  1:893449/1‑239 (MQ=255)
                                                                                                                                                                                                                      gtgaagtgtagggtttCATATTTTATATTTAGAAAAAGAGAAGAAAAAAAAAGAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCgg                               <  1:979297/225‑1 (MQ=255)
                                                                                                                                                                                                                       gtgtacagatggttttaattttttAAATTAAAATAAAGAGAGAAAAAATAAGAAAAAAAAGCCCGTCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCTACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCgg                               <  1:97201/220‑1 (MQ=255)
                                                                                                                                                                                                                          aTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa                                                                                                                                                              >  1:99190/1‑107 (MQ=255)
                                                                                                                                                                                                                            agagggaaattacaTTTTTAATATAAAAAAAAAGGAGAAAAAAAAAAAAAAAACACGCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAt                          <  2:256476/228‑1 (MQ=255)
                                                                                                                                                                                                                                agatacattttttttAATTGAGAAAAAGGGAGAAAAAAAACAAAAAAAACCCCCGCCATAACAGCGGGAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc                     <  2:859023/229‑1 (MQ=255)
                                                                                                                                                                                                                                 gAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCACAAGGTTGATTTTATCGTCAACGTAGTTGGCCATGTTTTTATTGAAATAGAAAGTTCCGCCAATATCGGGGGATTTaaac                    >  2:904960/1‑237 (MQ=255)
                                                                                                                                                                                                                                      cacaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCAACAGGGTGATTTTATAGTCAACGTAGGTGGACAGTTTTTTATTGAAAAAGAAAGTGGCGCCAAAATAGGCGAATTTAACCaaa                 >  2:1092095/1‑235 (MQ=255)
                                                                                                                                                                                                                                            tttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACAt                                                                  >  1:213943/1‑182 (MQ=255)
                                                                                                                                                                                                                                             taaaTTGAGATAAGAGGAAGAAGAAAAAACAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatca        <  1:412392/237‑1 (MQ=255)
                                                                                                                                                                                                                                                    tAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatctgtttct  >  1:859023/1‑232 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
ACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGT‑‑AATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACA  >  NC_000913/1435467‑1435940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: