Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,435,706	T→A	17.5%	intergenic (‑87/+54)	uspF ← / ← ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,435,706	0	T	A	17.5%	174.9 / 7.8	74	intergenic (‑87/+54)	uspF/ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base T (55/4);  new base A (2/11);  total (58/16)
Fisher's exact test for biased strand distribution p-value = 3.11e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACA  >  NC_000913/1435485‑1435940                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             gggCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATTACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTAAAT‑TTAGATAAAA‑G‑GGGGAAGAaaaa                                                                                                                                                                                                                           >  2:399098/1‑239 (MQ=255)   gCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCACA‑G‑GAGGAAGAATAAGc                                                                                                                                                                                                                         >  1:1024639/1‑239 (MQ=255)    cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAAAGAGGCTAATCACGCGTTGAGTTAATTCTGAATCGGCAATAT‑CGAAAGGGACAAGAAACGGTCTGTT‑CATAAAA‑ACTCCTGTATTAGTATCCGCATAAAGTGAA‑ACGCCAGAGGAAACTTTTT‑TGGA‑‑AAAGAC‑GGGGTAAAAATGTTTAAT‑TTAAAAAAAA‑G‑GAGGAAGAAAAAACa                                                                                                                                                                                                                        >  2:604244/1‑239 (MQ=255)      gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGTAAATAT‑CGATAGGGACAAGAACCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGAATCCGCATAAAGTTAA‑ACGCCCGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑AAGGAAAAATAAGCaaa                                                                                                                                                                                                                      >  2:532745/1‑237 (MQ=255)       tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CCTAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑CAGCCCGATTACACTTTTT‑TTGT‑‑AA‑GACGGGAGTTCACATTTTTAAT‑TTAAGACAAA‑G‑GAGGAAAAATAAGcgaaa                                                                                                                                                                                                                     >  2:979297/1‑235 (MQ=255)       tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAgaagaa                                                                                                                                                                                                                     >  1:1093464/1‑239 (MQ=255)       tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GGGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGTAAGAATAAgacgaa                                                                                                                                                                                                                     >  1:555980/1‑234 (MQ=255)         aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGGGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGaaaa                                                                                                                                                                                                                   >  1:104927/1‑239 (MQ=255)            gTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑TTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAg                                                                                                                                                                                                                 >  1:471070/1‑238 (MQ=255)                tACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGc                                                                                                                                                                                                            >  1:219085/1‑239 (MQ=255)                     tCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGCAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGTTCAAT‑G‑GAGGAAGAATAAGCAGAAAAACCCCCCCATa                                                                                                                                                                                                        >  2:623170/1‑238 (MQ=255)                       aGGAAATGCACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGCTGAGTTAATTCTGAATCGGAAATAT‑CGACAGGTACACGCATCGCTCTGTT‑CATAAAA‑CCTCCGGTTTTAGAATCCCCAAAAAGTCTA‑ACGCCAGCTGGCACTTTTT‑GTGT‑‑AATAAC‑GGAGTTCACATTTTTAAT‑TTAAACCCAA‑G‑GCGGCAACATAAACAACAAAACCCCCCAATaaaa                                                                                                                                                                                                     >  2:789356/1‑237 (MQ=255)                       aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAATTAT‑CGATAGGGACAAGAGTCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑TTGT‑‑AATGAC‑GGCGTTCCCATTTTTAAT‑TTATATCAAA‑G‑GAGGAAGAATAACAAAAAAAAGCCCCCCAAAACa                                                                                                                                                                                                     >  2:412392/1‑239 (MQ=255)                       aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GCGGAAGAATAAGCAGAAAAAGCCCCCCATaaa                                                                                                                                                                                                      >  1:625926/1‑237 (MQ=255)                       aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACa                                                                                                                                                                                                     >  1:552642/1‑239 (MQ=255)                                       catcatCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTAACATTTTTAAT‑TTAGATCAAT‑G‑AAGGAAGAATAAGCAGAAAAAGACACCCAAAAAACCGGGCagagagattt                                                                                                                                                                                     >  2:97201/1‑231 (MQ=255)                                            tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGAGAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGCTGACACTTTTT‑GTGT‑‑ACTGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGAAGGAGGAGTTAGAAc                                                                                                                                                                                >  1:1037971/1‑239 (MQ=255)                                            tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAACAGAAAAAGCCCGCCCTAACACCGGGCAGGAGGATTTAGAAc                                                                                                                                                                                >  2:1033537/1‑239 (MQ=255)                                                    cgCCTCTCCCTCAACGGGGAAAATCACGGGTTGTATTAATTCTGAA‑CCGAAATGTGCGAAAGGAACAAGAATCGTTCTGTT‑AAAAAAC‑CCCCCTGTTTGAGTACCCGATAAAAGTGAA‑CCGCAAGAGGACCTTTTTT‑GTTA‑‑AATGAG‑GGAGTTCACATTTTAAAT‑TAAGATCAAG‑G‑GAGGAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                                                                                                                                        <  1:623170/238‑1 (MQ=255)                                                     gCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGCGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATATGCAGAAAAAACCCGCCATAACAGCGGGCAGGAGGGTTTAAAAACGAAAAAca                                                                                                                                                                       >  2:478216/1‑238 (MQ=255)                                                      ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAAAAGCGGGCAGGAGGATTTAGAAATGAAAAAcaa                                                                                                                                                                      >  2:180703/1‑237 (MQ=255)                                                          ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCCTAACAGCGGGCAGGAGGATTTAGAACTGATaaaccaaacc                                                                                                                                                                  >  1:432727/1‑239 (MQ=255)                                                                aaCGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCCGAAAAAGCCCGCCATAAAAGTGGGCAGGGGGGTTTAGAACTGAAAAACCAgcaccaaaac                                                                                                                                                            >  2:710805/1‑230 (MQ=255)                                                                    tGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGCGTA‑ACGCCAGATGACACTTTTT‑TTGT‑‑AAGGAC‑GGAGTTAACATTTTTAAT‑TTAGATCAAa‑g                                                                                                                                                                                                                                        >  2:319405/1‑160 (MQ=255)                                                                      gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      >  1:253979/1‑239 (MQ=255)                                                                       ttaatcctggtttGATTTATTTCTCAACGAGAATTAG‑CGATAGGGAAAAAAATCGTT‑TCTT‑CAAAAAACCCTCTTTTTTTATTTACCGAAAAAAGTGAA‑ACGCCAGATGAC‑TTTTTT‑GTTTA‑AAGGAC‑GGATTTATCATTTTTAAT‑TAAGAAAAAG‑G‑GAGAAAAAATAAGCAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  2:859144/227‑1 (MQ=255)                                                                       taaagcacgatgtGATTTAATTGTGAAGCGAAAATAC‑GGATAGGGA‑AAAAAAGGTTTGGTTACATAAAA‑CCCCCTTTTTAATTATCCGCATAAAGTTAA‑ACGCGAGATG‑GACTTTTTGGGGA‑‑AATGAC‑GGAGTCAACATTTTAAAT‑TAAGACCAAG‑G‑GAGAAAGAAGAAGAAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTGAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  1:789356/226‑1 (MQ=255)                                                                        taaaactcgtttGTGTTTATTTTTAAGCGGAAATTT‑CGAAAGGGAAAAGAGTTTTTTTGTT‑AAAAAAC‑CCTCTTTTTTTTGTCTACCCA‑AAAATGTATAAGCCA‑AAGAGACCTTTT‑GGGTAAAAGGGG‑GGAGTCCACTTTTTAAAT‑TTAGAAAAAG‑G‑GGAAAAGAAAAAGAAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAat                                                                                                                                                      <  2:601421/228‑1 (MQ=255)                                                                                cgTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACATCGGCAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                                                                                                                            >  1:606007/1‑239 (MQ=255)                                                                                cgTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                                                                                                                            >  1:882729/1‑239 (MQ=255)                                                                                   tGATTTATTTCTAAAGCGAAAATAT‑GAATAGGCAAAAAAATCTTTCTGTT‑AATAAAC‑CCCCCTTTTTAAGTATCCAAAAAAAGTGAA‑CCGCAAGATGACTCTTTTT‑GTGT‑‑AATGAC‑GGAGTTCATATTTTTAAT‑TTAAAAAAAA‑G‑GAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTagag                                                                                                                                         <  1:180703/239‑1 (MQ=255)                                                                                               gAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCgg                                                                                                                                              >  1:338971/1‑222 (MQ=255)                                                                                               gAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCa                                                                                                                             >  1:714493/1‑239 (MQ=255)                                                                                               gAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAACTAAAGCGAAAATAACACAGGTCGAGATGACAATggaa                                                                                                                             >  2:238290/1‑237 (MQ=255)                                                                                                aaTCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                                                                                                                            >  1:256476/1‑239 (MQ=255)                                                                                                      aaaTAT‑GGAGGGGAAAAAAAATTGTTCTTTA‑AAAAAAC‑CCTCTTTTTTTAGTTCCCAAAAAAAGTTAA‑CCGCAAGATGA‑ATTTTTT‑GGGTA‑AAGGAG‑GGAGTTCACATTTTAAAT‑TAAGAAAAAA‑G‑GAGGAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg                                                                                                                       <  1:604244/238‑1 (MQ=255)                                                                                                        atgtgtaaaagagACAGGATTTGTTGTTTT‑CATAAAC‑CCTCCTGTTTTAGTACCCGAATAAAGTGAA‑ACGCCAGATGACTTTTTTT‑GTAT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TAAAATAAAG‑G‑GAGAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                                                                                      <  1:215378/226‑1 (MQ=255)                                                                                                             cGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAAATGCCATTGGCAGCGTAGAAGcagc                                                                                                                >  1:463793/1‑236 (MQ=255)                                                                                                                         gAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCAACAATACCAACGGGAAAGAAGCCATTGGCAGCGAAAAAACGGTCAACttacacca                                                                                                    >  2:885005/1‑233 (MQ=255)                                                                                                                             cGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAACGAAAAAACTGTAAAATTCATCCAAAAg                                                                                                >  2:374573/1‑239 (MQ=255)                                                                                                                               ttCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGc                                                                                                                                                                                               >  1:930785/1‑142 (MQ=255)                                                                                                                               ttCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt                                                                                              >  1:539514/1‑239 (MQ=255)                                                                                                                               ttCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGGGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGCAGCATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTATAAGCTGTCATCTTCATCCAACAGGt                                                                                              >  1:711003/1‑239 (MQ=255)                                                                                                                                 cTGTT‑CATAAAA‑CCTTCTGTTTGAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGCGTTCACATTTTTAAT‑TTATATCAAA‑G‑GAGGAAGAATAAGCAGACAAAGCCCGCAATAACAGCGGGAAGGAGGATTTCGACCTGATAAACAAGAACTAAAGCGAAACTATCATCGGTAGAGATGCCATTGGCAACTAAAAAGGAGTCATCTTCAACCAAAAGGTTg                                                                                            >  2:794111/1‑239 (MQ=255)                                                                                                                                    tt‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAACGGCAATATCATCGGTAGAGATGCCATTGGCAGCGAAAAAGCTGTCacccttgtcccttaaaaaaaac                                                                                         >  2:215378/1‑218 (MQ=255)                                                                                                                                             a‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc                                                                                 >  1:678399/1‑239 (MQ=255)                                                                                                                                                   ctGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCAGTCATCTCAATCCAACAGGTGGATTTTATAGTCAACga                                                                            >  1:232079/1‑238 (MQ=255)                                                                                                                                                          attccccaaaaatagtaga‑aCGCCAGATGCCTTTTTGT‑GGTT‑‑AATCGC‑GGGGTTCAAATTTTTAAT‑TAAAATCAAG‑G‑GAGAAATAAAAAGAAGAAAATGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGa                                                                     <  1:363697/221‑1 (MQ=255)                                                                                                                                                            tATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAATCTGTCATCTTCATCCAACAGGTTGATTTTATATCAAAAGAAGGTGGaaa                                                                   >  2:518043/1‑237 (MQ=255)                                                                                                                                                             aTCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGAAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGTTTTTATAGTCAACGTAGGTGGACAt                                                                  >  1:986202/1‑239 (MQ=255)                                                                                                                                                                cGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa                                                                                                                                                                                 >  1:726683/1‑125 (MQ=255)                                                                                                                                                                cGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGCCATGtt                                                               >  2:869/1‑239 (MQ=255)                                                                                                                                                                cGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                               >  1:760898/1‑239 (MQ=255)                                                                                                                                                                cGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAGGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGACAACGAAGggggcaaagtt                                                               >  2:742410/1‑232 (MQ=255)                                                                                                                                                                          gtA‑ACGCCAGCTGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GTGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACCGATAAACCAGACCTAAAGCGACAATATCATCGGTATGGATGCCATTGGCACGATAGAAACTGTCATCTTCCTCCAAAAGGCTGATTTTATAGTCAACGAAGGGGGACATGTTTTTTATGaaa                                                     >  2:989249/1‑239 (MQ=255)                                                                                                                                                                            gtaacccaaaatgcacTTTTTGGTGA‑‑AATGAC‑GGATTTCACTTTTTTAAT‑TTAGAAAAAG‑G‑GAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGaa                                                      <  1:885005/225‑1 (MQ=255)                                                                                                                                                                            a‑aCGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATa                                                   >  2:332021/1‑239 (MQ=255)                                                                                                                                                                              aCGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                  >  1:412979/1‑239 (MQ=255)                                                                                                                                                                              aCGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAg                                                  >  1:980520/1‑239 (MQ=255)                                                                                                                                                                                    gATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa                                                                                                                     >  1:428107/1‑166 (MQ=255)                                                                                                                                                                                         ttgttttt‑GTGA‑‑AATGAC‑GTAGTTCAATTTTTTAAT‑TAAAAACAAA‑G‑GAGAAAAAATAAGCAAAAAAACTCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCgcgc                                        <  1:264654/235‑1 (MQ=255)                                                                                                                                                                                          acTTTTT‑GTGT‑‑AATTAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGAAGGAGTTAGAAGTGATAAACCAAGCCGAAAGCGACAATATCATCGGTAGAGATGCCATTGGGACCGTAGAAGCTGTCATCTTGATCCAACAGTTTAATTTTATCGTCAACGTAGGAGGCCATGTTTTTATTTAAAAAAAAAACCGCGCCa                                      >  2:648930/1‑239 (MQ=255)                                                                                                                                                                                                  ttttttgtatggacggtttatattttttAAA‑TTAAAAAAAAGG‑GAGAAAAAAAAAACAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAata                                   <  2:760898/216‑1 (MQ=255)                                                                                                                                                                                                     t‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGt                            >  1:893449/1‑239 (MQ=255)                                                                                                                                                                                                     gtgaagtgtagggtttCATATTTTATAT‑TTAGAAAAAG‑A‑GAAGAAAAAAAAAGAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCgg                               <  1:979297/225‑1 (MQ=255)                                                                                                                                                                                                      gtgtacagatggttttaattttttAAA‑TTAAAATAAA‑GAGAGAAAAAATAAGAAAAAAAAGCCCGTCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCTACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCgg                               <  1:97201/220‑1 (MQ=255)                                                                                                                                                                                                         aTGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa                                                                                                                                                              >  1:99190/1‑107 (MQ=255)                                                                                                                                                                                                           agagggaaattacaTTTTTAATATAAAAAAAAA‑G‑GAGAAAAAAAAAAAAAAAACACGCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAt                          <  2:256476/228‑1 (MQ=255)                                                                                                                                                                                                               agatacattttttttAAT‑TGAGAAAAAG‑G‑GAGAAAAAAAACAAAAAAAACCCCCGCCATAACAGCGGGAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc                     <  2:859023/229‑1 (MQ=255)                                                                                                                                                                                                                gAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCACAAGGTTGATTTTATCGTCAACGTAGTTGGCCATGTTTTTATTGAAATAGAAAGTTCCGCCAATATCGGGGGATTTaaac                    >  2:904960/1‑237 (MQ=255)                                                                                                                                                                                                                     cacaTTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCAACAGGGTGATTTTATAGTCAACGTAGGTGGACAGTTTTTTATTGAAAAAGAAAGTGGCGCCAAAATAGGCGAATTTAACCaaa                 >  2:1092095/1‑235 (MQ=255)                                                                                                                                                                                                                           tttAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACAt                                                                  >  1:213943/1‑182 (MQ=255)                                                                                                                                                                                                                            taaaT‑TGAGATAAGA‑G‑GAAGAAGAAAAAACAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatca        <  1:412392/237‑1 (MQ=255)                                                                                                                                                                                                                                   tAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTatctgtttct  >  1:859023/1‑232 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAT‑CGATAGGGACAAGAATCGTTCTGTT‑CATAAAA‑CCTCCTGTTTTAGTATCCGCATAAAGTGTA‑ACGCCAGATGACACTTTTT‑GTGT‑‑AATGAC‑GGAGTTCACATTTTTAAT‑TTAGATCAAA‑G‑GAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACA  >  NC_000913/1435485‑1435940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑