Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,164 A→T 26.6% G154G (GGT→GGA spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,1640AT26.6% 128.3 / 11.9 77G154G (GGT→GGAspyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base A (33/21);  new base T (14/8);  total (48/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCA  >  NC_000913/1824933‑1825399
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTGTTCAGTTTTTTTGTCTAAAAAATATTCAACAGTTGCAGGACCTTTAACCttt                                                                                                                                                                                                                                             >  1:459776/1‑238 (MQ=255)
tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGTTTTTTGGCTGTAAAATTATTTAACAGTTTCCGGCATTTTTCCtttt                                                                                                                                                                                                                                            >  1:1073144/1‑238 (MQ=255)
         gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGGTTTTTGGCTGAAAAACTATTCTGCAAGTGCCGGCACGTTTCCTTTTTCAGCTGGaa                                                                                                                                                                                                                                  >  1:870877/1‑238 (MQ=255)
         gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGGTGTAAGATTATTTAGCAGTTGCAGGCATTTTTCCTTTTTCCGCTggcg                                                                                                                                                                                                                                  >  1:417443/1‑237 (MQ=255)
                gCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTTAATTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTTCGCTGGGCGt                                                                                                                                                                                                                                >  1:258688/1‑234 (MQ=255)
                     ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTCAGTTTTTTTGCTGAGAAAATATTCAACAGATGCGGGGACTTTCACTTTTGCAGCCGGGCGGTCtttgctac                                                                                                                                                                                                                      >  2:869339/1‑232 (MQ=255)
                                  tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGAACAAGACCGGCGGGCTTAAACTTTTTTGCTGAACAGTTTATTCAGAATTGCAGCGACTTTTCCTTTTGCCGCTGGAAGTTTTTTCAAACGCCTCTCAAAAtt                                                                                                                                                                                                       >  2:591102/1‑239 (MQ=255)
                                                      aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGGTTTTTTGCTTGAAGATTATTTACCCGGTGTAGGCATTTTTCCTTTTTACGCCGGTCGTGTTTTTAGACGCTTCTCAAAATATTCATTAAAATGAttttttt                                                                                                                                                                                   >  2:349983/1‑237 (MQ=255)
                                                             tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAGGACCGGCGGTCTTTAATTTTTTTGATTGAAAATTATTTAGCAGTTGCAGGCATTTTTCCCTTTTCCGCCGGACGTTTTTTCTGACGCGTTCCAAAAATAGAATTAAATTATTTTTTTTTTTTCgg                                                                                                                                                                            >  2:565345/1‑239 (MQ=255)
                                                                  aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAACAGTTGCCAGCCTTTTTCCTTTTGCCGCTGGACGTTTTGTCCAGAGCTTCTCAAAATTAACATTAAATTGCTTTTTTTTTTCCGGCGTCa                                                                                                                                                                      >  1:411123/1‑239 (MQ=255)
                                                                                      gtgtgtagggttttttttttACAATTTGTTTAAAAAAAAAAAAAAACCCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGtt                                                                                                                                                   <  2:17219/226‑1 (MQ=255)
                                                                                      gtggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCATAAGGTTTTTGGCTGGAAGATTATTTAGCACTTGCGGGCCACTTACCACTTGCCCCGGGACGATTCATGAGACCCGTCCCTAATTTTCCATTTAATTATCTTTTTTGTTCCCGCCGCCGGATGTTTTAAAAttttttt                                                                                                                                                  >  2:78995/1‑238 (MQ=255)
                                                                                      gtggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTTGAAGCCTTTTACCCTTTTCCGCTGGACGTTCTGTCAGACGCTTCCCCAAAATAGACTTTAAATGCTTTTTTTTTTTCGGCggccgggtttgggaaaaatttttt                                                                                                                                                   >  2:1090285/1‑216 (MQ=255)
                                                                                           gATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGGCGTTCTGTAAGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCCGCGTCAGGATGTTGGTAATTTTGTTCTTGGt                                                                                                                                             >  1:781501/1‑239 (MQ=255)
                                                                                            aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCTCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGCTGCAGGGCTTTTACCTTTTGCCGCTGGACGTTCTGGCCGACCCTTCCCAAAATTCACATCTAATTGACTTTTTTGTTCCGGCGGCCGGGTGTTGTAAATtttttttgtggtt                                                                                                                                            >  1:627361/1‑232 (MQ=255)
                                                                                              cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGTCCGGATGTTGTAAATTTTTTTTTGGGgtt                                                                                                                                           >  1:961401/1‑238 (MQ=255)
                                                                                              cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTGGAAGATTATTCAGAAGTTTCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTTAGACACTTCTCCAAATTATCATTATATTGCGTTTTTTGTTCCGGCGTCCAGGAGGTGTAAAATTTTTTCTTGGgtt                                                                                                                                           >  2:57033/1‑238 (MQ=255)
                                                                                                 gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACAGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTCAGTTTTTTTGCTTAAAGATTTCTTCGCAGTTGCAGGGCTTTTACCTTTTTCCCCTGGAGGGTCTTTCCTACGCTTCTCAAAATATCCTTTTAATATCCTTTTCTCTTCTCGCGTCAGGCTGTTGTAAATTTTTTTTTGGGtttgcct                                                                                                                                       >  2:5770/1‑235 (MQ=255)
                                                                                                   tttttttAGATTTGTTGAGAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGGCCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCAtgt                                                                                                                                    <  1:284529/239‑1 (MQ=255)
                                                                                                    ttttttACAACTGTTTGAGAAGAGAAGAGAAAACCGCCGCTCCTGTACACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTATGTGTTTTTGCTGGAGAATTTTTTCGCATGTTAGGGGCTTTTAACTTTTTCAGATGGAACTTCTCTCAGACGCCTCTCCAACTTACCGTTTAGTTACCTTTTTTCTTCGCGCGTCAGTGTAATTTCAATTTTGTTCTGGGTTTctagtt                                                                                                                                     >  2:67682/1‑233 (MQ=255)
                                                                                                    ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGGTTTTTTGCGGAAAAATTTTTTAGCAGTTTCAGGGATTTTTCCTTTTGTCACTGGACGTTCTTTCCGAAGGCTCTCAAAATTAGCACTTTATTTTTTTTTTTTTTTCGGTGCCAGGGTGTTTTAAAATTTTTTTTGGGGTTccctttt                                                                                                                                    >  2:265947/1‑234 (MQ=255)
                                                                                                      ttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGCCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGGAGGCCATTTACCTTTTGCCGCTGGACGTTCTGTCAGAGCCTTCTCAAAATTAGCAGTAAATTTCTTTTTCTGTTCCGGCGGCAGGATGTTGGAAATTTTTTTCTTGGGTTCCATTTgc                                                                                                                                  >  1:1039613/1‑238 (MQ=255)
                                                                                                       ttcaCAATTGTTGGAAAGAAAAAAAAAAACCCGCCGATCCTGTCCTCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTgcgc                                                                                                                                <  2:1073144/236‑1 (MQ=255)
                                                                                                                 gTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACATTTTGCCGCTGGACGTTTTGTCAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTGTTCCGGCGTCAGGGTGTTGTTAATTTTGTTTTTGGGTTCCATTTTCGCCAGctggtt                                                                                                                       >  1:1105200/1‑234 (MQ=255)
                                                                                                                  tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTTAGCCGTTGTAGGCCATTTACCTTTTTCCGCTGGGCGGTCTGTCAAAACGCTCTCCAAATTATCATTTAATTTCCTTTTTTTTTCCCGCGGCCAGTTCTTTACAATTTTTTTCTGGGTTTccctttgtcccacgcagtta                                                                                                                       >  2:597457/1‑221 (MQ=255)
                                                                                                                  tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTATCATTAAATTGCTTTTTCTGTTCCCGCGTCAGGATGTTGTAAATTTTGTTCTGGGGTTCCCTTTGCGCCAGCATTTTa                                                                                                                      >  1:686041/1‑239 (MQ=255)
                                                                                                                  tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGGTGAAAGATTATTCAGCAGTTTCAGGGATTTTTCCTTTTTCCGCTGGACGTTCTGTCAAACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTTTGGGTTTTCCTCTTCGCCCGCATGTTa                                                                                                                      >  1:115633/1‑239 (MQ=255)
                                                                                                                    tGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAATTTTTTTGCTGAAAGATTATTTAGCAGATTCAGGCATTTTTCCTTTTTTCCCTGGACGTTTTGTTCGACGCTTCTCAAAATTTACATTAAATTTCTTTTTTTTTTTCGGCGCCCGGGTGTTGTAAATTTTTTTTTTGGTTTctcttttgcccacactgtgtga                                                                                                                     >  2:345574/1‑218 (MQ=255)
                                                                                                                    tGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTTTGTCAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTTTGTTCCGGCGTCATGTTGTTGTAAATTTTTTTCTGGGGTTCCATGTGCGCCAGCATGTTa                                                                                                                      >  1:180373/1‑237 (MQ=255)
                                                                                                                             aaaaaaaaacCCGCCGATCCTGTCCACCGCATTACGGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgcg                                                                                                             <  1:591102/236‑1 (MQ=255)
                                                                                                                                              aTCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAAACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTTTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTTTCCttttttc                                                                                           >  1:550758/1‑237 (MQ=255)
                                                                                                                                               tCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGcc                                                                                                                               <  1:1022347/203‑1 (MQ=255)
                                                                                                                                                        ccggtttACTTAAAGGTGGGGCACAAGCCCGGCGGTTTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAATATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCt                                                                                 <  2:550758/234‑1 (MQ=255)
                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAAGTTTTTTGGTGAAAGAGTATTTAGCAATTTTCAGCAGTTTTCCTTTTTCCTCTGGGAGTTCTTTCAAACGCCTCTCAAAAATAACATTAAAATCATTTTTTTTGTCCGCGGTCAGGAATTTTGCAATTTTGTTTTGGGTTTCCACTTTCCGCCCGCTTTTAGATTTTCACTTTTTTTCCATTTTTTCGCTATgcgcc                                                                                 >  2:964428/1‑237 (MQ=255)
                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTTAAAGATTTTTCAGCAATTTCAGGCATTTTTCCTTTTTCCGTTGGACGTTCTTTCAGACGCTTCTCAAAATTAGCATTAAATTTCCTTTTCTTTTCCGGCGTCAGGTGGTTGTAAATTTTGTTCTGTTGTTCCATGTGCCCCGCCATGTTAACCTTATGCTGTTCTCCCTTTTTTGCgtacttgcct                                                                                 >  1:557899/1‑229 (MQ=255)
                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTAACTTTTTTCGCTGGACGTTTTGTCAGACGCTTCTTCACAATAGCATTAAATTTCTTTTTTTGTTCCGGCGTCAGGATGTTTTAAATTTTGTTCTGGGTTTCCCTGTTGGCCAGCCTGTTAGCTTTTTGGCTGTCTTCCCTTTTTGCGATCTGCCCt                                                                                 >  1:502680/1‑238 (MQ=255)
                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGCTTTTTTGCTGAAAGATTATTTAGCAGTTGCGGGCATTTTAACTTTTTCCCCTGGACGGTCTGTCAGACCCCTCTCCAAATTAGCCTTAAATTTCCTTTTCTTTTTCGGCGCCCGGGTGTTTTAAATTTTGTTTTTGGGTTCCATTGGCGCCAACATGTTTGCGTTTCGCTGTTCTTCCCttttttctgttttcgct                                                                                 >  2:587453/1‑226 (MQ=255)
                                                                                                                                                          gCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                                                               <  1:837034/239‑1 (MQ=255)
                                                                                                                                                                 tGCAAGGTAGTGGACAAGCCCGGCGGTCTTAAGTTGTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAg                                                                                                                             <  1:782513/187‑1 (MQ=255)
                                                                                                                                                                   cAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGcc                                                                                                                               <  1:5937/183‑1 (MQ=255)
                                                                                                                                                                    aaGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCGGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                     <  1:587453/239‑1 (MQ=255)
                                                                                                                                                                      ggTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTTCCGCTGGACGTTTTGTCAGACGCGTCTCAAAATTTGCATTAAATTGCTTTTTTTGTTTCGGCGTCAGGATGTTGTTAATATTTTTTTTGGTTTTCCTTTGCGGCAGCATGTTTGATTTGTGCTGTTTTTTCATTTTTTTGATTTGCGCTTTAGCTTTTACttt                                                                   >  2:445767/1‑239 (MQ=255)
                                                                                                                                                                            ggACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCg                                                               <  1:386437/237‑1 (MQ=255)
                                                                                                                                                                                aaGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTTACTTTTGCCGCTGGACGTTCTTTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTTCCGGCGTCCGGCTGTTGTAAATTTTGTTCTTGGTTTCCATTTGCGCCAGCATGTTAGCTTTTCGCTGTTTTTCCCTTTTTTGGTTCTGGGCTTCACCTTTTACTTTTTCGAAGGTa                                                         >  1:863742/1‑239 (MQ=255)
                                                                                                                                                                                aaGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCCGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAt                                                                                                                                                                                                        >  1:1059277/1‑99 (MQ=255)
                                                                                                                                                                                aaGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAt                                                                                                                                                                                                        <  2:1059277/99‑1 (MQ=255)
                                                                                                                                                                                  gACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCCGCGTCCGGAGGTTTTAAATTTTGTTTTGGGTTTTCCTGTTCGCCAGCATGTTTGCTTTTCGCTTTTTTTCCATTTTTTCGGTCTTCCCTTCAGCttttttttttttagagggaa                                                        >  2:1085902/1‑222 (MQ=255)
                                                                                                                                                                                  gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGGCCGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATTTTCGCTTCAGCTTTTACTTTTTCTAAGGTAt                                                        >  1:1099406/1‑238 (MQ=255)
                                                                                                                                                                                  gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAACATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTTTGGGTTTCCATTGGCGCCAGCATGTTAGCTTTTCGCTGTTCTTCCATTTTTGCGGTCTTCGCTTCAGCTTTTACTTTATCGAAGGTAt                                                        >  1:543887/1‑238 (MQ=255)
                                                                                                                                                                                  gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTTACTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGGCCGGTTGTTGGAAATTTTTGTTTTGGTTTCCCTGTGGGCCAGCCAGGTAGATTTTCGCTTTTTTTTCATTTTTTCGATCTGCGCTTTAGCTTTTATTTTATCGAAggggta                                                       >  2:370180/1‑235 (MQ=255)
                                                                                                                                                                                     cggcggTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                    <  1:565345/239‑1 (MQ=255)
                                                                                                                                                                                         gggtttttattttttttGGCTTAAAGATTATTCAACAGTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCCGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTgg                                                  <  2:615616/228‑1 (MQ=255)
                                                                                                                                                                                           tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                               <  1:345574/238‑1 (MQ=255)
                                                                                                                                                                                           tCTTAAGTTTTTTGGCTGAAAGATTAATCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                               <  1:850006/238‑1 (MQ=255)
                                                                                                                                                                                            cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  1:142646/239‑1 (MQ=255)
                                                                                                                                                                                            cTTAAGTTGTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  1:349983/239‑1 (MQ=255)
                                                                                                                                                                                             tttAAGTTTTTTTGGTTAAAGATTATTCAGCAGTTGTATGCATTTTTCCTTTTGCCGGTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:180373/238‑1 (MQ=255)
                                                                                                                                                                                             tttAAGTTTTTTGGCTGAAAGTTTATTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:543887/238‑1 (MQ=255)
                                                                                                                                                                                             tttAAGTTTTTTGGCTGAAAGATTTTTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:258688/238‑1 (MQ=255)
                                                                                                                                                                                              ttAAGTTTTTTGGCTGAAAGTTTATTCAGCAGTTGCAGGCATTTTACCTTTTGGCGCTGGTCGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  1:727850/238‑1 (MQ=255)
                                                                                                                                                                                              ctttatttttttGGGTTAAAGATTTTTCAGCCGTTGCAGGCATTTTTCCTTTTGCCGTTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:1099406/233‑1 (MQ=255)
                                                                                                                                                                                                       tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATTTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGGATCGCTGGCAATGATGTCTTGc                                    >  1:431016/1‑239 (MQ=255)
                                                                                                                                                                                                              gAAAGTTTTTTCAGTAGTTTGAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                             <  2:627361/239‑1 (MQ=255)
                                                                                                                                                                                                               aaaGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTATATTGCTTTTTCTGTTCCGGTGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCTCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGTCCTGCGCTTACGCTTTTCCTTTATCGAAGGTAACACTGCCatagttttttgcccttggcag                            >  2:1063730/1‑219 (MQ=255)
                                                                                                                                                                                                                taaatgttttttcgccgtggCAGGCTTTTTTCCTTTTGCCTCTGGACTTTCTGTCAGACGCTTCTCAAAATTAGCTTTAAATGGCTTTTTCTGTTCCGGCGTCAGGATGTTTTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                             <  2:577710/220‑1 (MQ=255)
                                                                                                                                                                                                                    attattCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGt                        <  1:1085902/239‑1 (MQ=255)
                                                                                                                                                                                                                           agcagTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCa                 >  1:710458/1‑239 (MQ=255)
                                                                                                                                                                                                                                 ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTTTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                <  2:271669/171‑1 (MQ=255)
                                                                                                                                                                                                                                 ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                >  1:271669/1‑171 (MQ=255)
                                                                                                                                                                                                                                 ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggc            >  1:734860/1‑239 (MQ=255)
                                                                                                                                                                                                                                 ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGAGATCTGAGCTTCAGCTTTTACTTTATCGAAGGCATCGCTGGCAATGATGTCATGCATTTCGCGGCGTTCTTCCatccgc            >  1:297651/1‑234 (MQ=255)
                                                                                                                                                                                                                                  tGCTGGCTTTTTTCCTTTTGCCGCTGGACTTTCTTTCAGACGCTTTTCAAAATTGACATTAAATTTCTTTTTCTGTTCCGGCGTCAGGATTTTTTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCTCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg           <  2:781501/239‑1 (MQ=255)
                                                                                                                                                                                                                                   gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgcg                                                                                                             <  2:814207/140‑1 (MQ=255)
                                                                                                                                                                                                                                   gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgcg                                                                                                             >  1:814207/1‑140 (MQ=255)
                                                                                                                                                                                                                                   gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGcagcgg          >  1:928058/1‑239 (MQ=255)
                                                                                                                                                                                                                                          aTTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCATCGCCGGACGttt    >  1:497480/1‑239 (MQ=255)
                                                                                                                                                                                                                                            tttACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCACTGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCa  >  1:1058297/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCA  >  NC_000913/1824933‑1825399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: