Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,007,105	T→G	23.2%	V317G (GTG→GGG)	amyA →	cytoplasmic alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,007,105	0	T	G	23.2%	121.1 / 2.7	68	V317G (GTG→GGG)	amyA	cytoplasmic alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base T (30/18);  new base G (5/14);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTATAAAGAGTGGATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGA  >  NC_000913/2006885‑2007333                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       ttttATAAAGAGTGGATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTGATAAGCTGCAAAAGGATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCCA‑GAAGCATCGCGAATGGGGCGC‑GATTCAGCAATGACGCAG‑CGTTTTA‑C‑GGGGAACTTAGGAGAAGCCggacattttc                                                                                                                                                                                                                       >  1:426608/1‑229 (MQ=255)               gATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCCG‑ATTTTCC‑C‑GGGGACATTAGTGGAAGCCG‑ATCCTTTCCACCCCGGGAcccccg                                                                                                                                                                                                       >  1:64565/1‑234 (MQ=255)               gATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTAATAAGCCGCCAACGTATATTGGTCAGGTGGAAGGCAAAACCAAGCTGTTTGATGCACCGCTGAAGCTGAAATTTCAT‑TACACATCGCGCATGGGGCGC‑GGCTACGACAGTACCCAG‑ACTTTCA‑C‑GGGGACATTAGGGGAAGACG‑AACCCTTCCCCCCCGCGAccccgg                                                                                                                                                                                                       >  2:398875/1‑234 (MQ=255)                     acacGTTAAGGAATTTGCGCCAAAGCCGCTTGTTATTGTGGCGGGTGTCTGGGCGGAAGAAGTTGATAAGCTGCAACCGGTTTTTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCa                                                                                                                                                                                                 <  2:60160/239‑1 (MQ=255)                         tttcAGGAATTTGCGCCAAATCCGTTTTTTATTGTGGCGGTGTTCGGGTCGCATGAATTTGATAAGCTGCAAACGTTTATTGAGTAGGTGGAAGGGAAAACCATGCTGTTTGATGCGCCGCTGGAGATGAAATCCCAT‑GAAGCGTCGCGCATGGGGCGC‑GTCTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGGGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCa                                                                                                                                                                                             <  2:452363/236‑1 (MQ=255)                            cgggagatggtgataagggacaggTTTATTGTGGCGGAGTACTGGTCGAATGAAGTTGATAAGCTGAAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACg                                                                                                                                                                                           <  1:379710/215‑1 (MQ=255)                                   ttGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTGATCAGCTGCAAACGTATATTGATCAGGAGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATTAAATTCCAT‑GAAACATCGCGCATTGGGAGC‑GACTACGACATGACGAAGCATTTACA‑‑‑GGGGACATTAGGTGAAGCCG‑AACATTTCCACCCCGTGACGCCCGTTGCAAATAACAACACCCa                                                                                                                                                                                    >  2:548954/1‑238 (MQ=255)                                   ttGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCCG‑ATTTTAA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCCCGTTGCCAATCCCGACACCCa                                                                                                                                                                                    >  1:790683/1‑238 (MQ=255)                                                         tgggggggAGTAATGGTCGGATGAAGTTGAGAAGCTGCAAACGTATATTGACCAGGTGGAAGGCAAAACCATGCTGTTGGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAgcgc                                                                                                                                                             <  1:548954/233‑1 (MQ=255)                                                            ggCGGAGTACTGGTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGGAACTTAGTGGAAGCCG‑GTCCTTTCCACGCCGTGGCGCTCGTTGCCAATCACGACAACCAACCGGTGGAAGACCCCGAAGAGCCgg                                                                                                                                                          >  2:545167/1‑239 (MQ=255)                                                                gAGTACTGGTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GCCTACGACATGACGCAA‑ATTTTCA‑C‑GGGGACATTAAGGGAAGCGG‑ACCCTTTCCCGGCCAGAACGCCCGGTGCAAATCACGACACCCAACCGTTCCAAGCCCTCGAGACGCCGGTcaa                                                                                                                                                      >  2:171807/1‑237 (MQ=255)                                                                       ggTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCgggg                                                                                                                                               >  1:396050/1‑239 (MQ=255)                                                                        gTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCCCGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGt                                                                                                                                              >  1:174380/1‑239 (MQ=255)                                                                        gTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAAcctcggt                                                                                                                                              >  1:373224/1‑234 (MQ=255)                                                                           gcttGAATTTAATAAGGGTCAAACGTATATTGATCAGGTGGAAGGCAAACCCAGGCTGTTTGGTGGCCCGCTGAAGTGGAAGTTCCAT‑AAAGCTCGGCGGAGGGGGGGC‑GCTTTGGCCATGACGGAG‑‑TTTTCACC‑GGGTACATAAGGGGAACCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCCCGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTa                                                                                                                                           <  2:804598/236‑1 (MQ=255)                                                                            caggAAGTTTAATAGTTGAAAACGTTGATTGATGAGGTTGAAGGCAAAACCGTGCGGTTTGTGGCGCCGTGGCAGATGAAATTCCAT‑GAAGGCTCGCGAGTGGGGCGT‑GACTTCGACATGACGCAG‑ATTTTCA‑C‑GGGTCCATTAGTGGAAGCCG‑ATCCTTTCCACGCTGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTa                                                                                                                                           <  2:395534/235‑1 (MQ=255)                                                                                       tAAGCTCCAAACGTATTTTGAACAGGTGGAAGGCAAAACCATGCTTTTTGAGGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGt                                                                                                                              <  1:545167/239‑1 (MQ=255)                                                                                           cTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCACCCCTTGCAAGCCCTCGAAGGCCCGGTCGACCCGTGGTTTAAACCCCAG‑GCGTATg                                                                                                                           >  1:226558/1‑238 (MQ=255)                                                                                             ggaacggtgttgtggtgaGGGGGAAGGAAAACCCAGGGTGGTTGGCGCGCCGCTGCAGGTGAAGTTCAGT‑GAAGCATCGCGGAGGGGGCGC‑GACTGCGACAGGCAGAAG‑GTGTTCA‑C‑GGGTACATTAGGGGAAGCCG‑ATCCTTCCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGcc                                                                                                                         <  2:655541/221‑1 (MQ=255)                                                                                             gCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGCCATGACGCAG‑ATTTTCA‑C‑GGGTCCATTAGGGGAGACCG‑ATCCTTTCAAGCCCGTGACGCCCGTTGCCAATCACCACACCCAACCGTACAAAGCCCTCCAAGCGCCGGTGGCAACGTGTGTTAAACCGCGG‑GCGAAAGcca                                                                                                                        >  2:246139/1‑238 (MQ=255)                                                                                              cAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GGAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCa                                                                                                                                                                           >  2:422786/1‑188 (MQ=255)                                                                                              cAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCa                                                                                                                                                                           <  1:422786/188‑1 (MQ=255)                                                                                                      tatTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGATACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCCG‑GCGTATGCCTTAATTttgt                                                                                                               >  1:251727/1‑239 (MQ=255)                                                                                                         tGACCAGGTGGAAGGCAAAACCTTCCTGTTGGAGGCGCCCGTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACg                                                                                                                                                                                           <  2:507183/161‑1 (MQ=255)                                                                                                             cAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGTAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAACGGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGa                                                                                                        >  1:902714/1‑239 (MQ=255)                                                                                                             cAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGa                                                                                                        >  1:10989/1‑239 (MQ=255)                                                                                                             cAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGGGGTTTAAACCGCTG‑GCGTATCCCTTAATTTTTTTGCGGGa                                                                                                        >  2:831211/1‑239 (MQ=255)                                                                                                             cAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGAGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACCCCGTGACGCTCGTTGCCAATCACGACACCCACCCGTTGCAAGCCCCCCAAACGCCGGTCCAACCCGGGTTTACACCGCCG‑GCGGATGCCTTAATTTTGTTGGGGGa                                                                                                        >  2:429743/1‑239 (MQ=255)                                                                                                              aGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGCACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGaa                                                                                                       >  1:810937/1‑239 (MQ=255)                                                                                                                gTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGACCCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGaaaa                                                                                                     >  1:342281/1‑239 (MQ=255)                                                                                                                gTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGCATGCCTTAATTTTGTTGCGGGaaaa                                                                                                     >  1:820377/1‑239 (MQ=255)                                                                                                                gTGGAAGGCAAAACCATGCTGTTTGATGCCCCGCTGCAGATGAAATTCCAT‑GAACCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTATAGGAAGCCG‑ATCCTTTCAACGCCGTGACGCTCGTTGCCAATCACGAAAACCCACCGCTGCAAGCCCTCAAAGCCCAGGTCGAACCGTAGTTTAAACCGCTC‑GCCTATTCCCTCATTTTTTTTCTGAaaaa                                                                                                     >  2:783318/1‑239 (MQ=255)                                                                                                                   gAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAAcc                                                                                                                                                                                 >  1:507183/1‑161 (MQ=255)                                                                                                                     aGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACCCCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGACCCGTGGTTTAAACCCCTGCGCG‑ATGCCTTAATTTTGTTGGGGGAAAAAGGCg                                                                                                >  2:201425/1‑239 (MQ=255)                                                                                                                         aaacCCAGGCGGTTTGATGCGCCCGTGCAGATGAAATTCCAT‑GAAGTATCGCGCATGGGGGGC‑GACTACGACAGGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTc                                                                                             <  1:429743/238‑1 (MQ=255)                                                                                                                         aaaaCCTTGCTTGTTGGTGGCCCGCGGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGA‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTc                                                                                             <  1:758216/238‑1 (MQ=255)                                                                                                                          aaaCCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGGGTTCCt                                                                                           >  1:72853/1‑239 (MQ=255)                                                                                                                             ccATGCGTTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCAGGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCg                                                                                        <  1:398875/239‑1 (MQ=255)                                                                                                                                tGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGGAAATGGCGTTCCTTggggt                                                                                     >  2:1063562/1‑237 (MQ=255)                                                                                                                                 gCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGGGCCGGTCGAACCGTGGGTTAAACCGCGG‑GCGTATGCCTTAATTTTTTTGCGGGAAAATGGCGATACCTCGGttt                                                                                    >  2:968877/1‑237 (MQ=255)                                                                                                                                       ttgcgccccccttcaggggAAATTCCAT‑TAAGCCTCCGCCTTGGGGCGC‑GCCGCCGCAATGTCCGAT‑ATTTTCA‑C‑GGGTACATTGGGGGAACCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCCCGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATc                                                                              <  2:342281/221‑1 (MQ=255)                                                                                                                                       tgtggctccgtggcaagtGAAATTCCAG‑GAAGCATGGCGCATGGGGGCC‑GACTACGACATGACGCAG‑ATTTCCG‑C‑GGGTACATTAGTGGAAGCCG‑TTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATc                                                                              <  2:72853/222‑1 (MQ=255)                                                                                                                                         gtgcccccctcgaaaTGAAATCCCTG‑GAAGCACCGCCCATGGGGCGC‑CTATCCGCCATGCCGCAG‑ATTTTCA‑C‑GGGTACATTAGGGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCg                                                                            <  2:820377/225‑1 (MQ=255)                                                                                                                                         gggccccccgggaagtgtaaTTCCAT‑AAGGCACGGCGC‑TGGGGGGCAGACTACGCCATGACGCAG‑ATTTTCA‑G‑GGTTCAATTAGGGGAGGCCG‑ATCCTGTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCg                                                                            <  2:823726/221‑1 (MQ=255)                                                                                                                                         aTGCGCCGCTGGAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGTTACATTAGGGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCg                                                                            <  1:691312/239‑1 (MQ=255)                                                                                                                                          ggccgctcgccagtggAAATTCCTT‑CGAGGACCGCGCGGGGGGCGC‑ACCGACGACAAGCCTCAG‑ATTTGCA‑G‑GGGTACATTGTTGGACGCCC‑ACCCGTCCCACGCCGGGACGCTCGTTGCCAGTCCCGACACCAAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGGTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCg                                                                            <  2:704128/223‑1 (MQ=255)                                                                                                                                               cgcTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGGATTCTATCCGGAcc                                                                        >  1:699906/1‑237 (MQ=255)                                                                                                                                                 aaaaggaacagatcccAT‑GAAGCCGCGCGGTTGGGGGGC‑GACTACGACATGACGCAG‑ATTTCCA‑C‑GGGTACATTAGGGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTa                                                                    <  2:916836/225‑1 (MQ=255)                                                                                                                                                   gCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCCC‑GACTACGACATGACGCAG‑ATTTGCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTCCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACg                                                                  <  1:1063562/239‑1 (MQ=255)                                                                                                                                                        tGAAATTCCATGGAAGC‑TCGCGCGTGGGGCGC‑GACTCCGACATGGCGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTgcg                                                             <  1:797585/239‑1 (MQ=255)                                                                                                                                                           aaTTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGCGGTTTAAACCGCTG‑GGGTTTGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGAATTCTATCCGGCCCTCTCCGGGGCCCAt                                                          >  2:285921/1‑239 (MQ=255)                                                                                                                                                                 aT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGag                                                    >  1:27534/1‑238 (MQ=255)                                                                                                                                                                   gaaacccgcggcgaTGGGGCGC‑GACTACGAATGGACCCAG‑ATTTTCC‑C‑GGGTACATTAGGGGAAGCCC‑ATCCTTGCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAg                                                   <  2:1079523/226‑1 (MQ=255)                                                                                                                                                                        cATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCAt                                                          >  1:916836/1‑227 (MQ=255)                                                                                                                                                                         aTCGCCGATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGGGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCg                                             <  1:201425/239‑1 (MQ=255)                                                                                                                                                                           cgcgCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGGATTCTATCCGGGCCTCTACGGTGCCAATTACAAAAGTGCCggt                                           >  1:1049807/1‑239 (MQ=255)                                                                                                                                                                             cgcATGGGGCGC‑TACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAgcgc                                                                                                                                                             <  1:777980/124‑1 (MQ=255)                                                                                                                                                                             cgcATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAgcgc                                                                                                                                                             >  2:777980/1‑124 (MQ=255)                                                                                                                                                                                aTGGGGCCG‑GACTACGACATGACGAAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑TTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGgtggtg                                       <  1:731389/238‑1 (MQ=255)                                                                                                                                                                                       ggccgactagctcattgccgaagttttCC‑G‑GGGTAAATATGGGGAACCGG‑CTCCTTCCCAACCCGTGACTCTAGTTGCCAATCACGACCCCCAACCGTTGCAAGCCCTGGAAGCGCCGGTCGAACCGTAGTTTAACCCGCGG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACggg                                  <  2:810937/215‑1 (MQ=255)                                                                                                                                                                                        gctactcgcccATGCCGCAT‑CTTTTCA‑‑‑GGGTTCAATTGTGGGAACCGCATCCTTCCCCCGCCGTGCAGCTCGTGGCCAAGCCAGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGTTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCaa                               <  2:226558/229‑1 (MQ=255)                                                                                                                                                                                          gactcggACATGAGGGAG‑‑TTTTCA‑CGGGGTCCATTAGGGGAAGCCG‑CTCCTTCCCACGCCGTGACGCTCGTTGCCAATCCCGACCCCCAACCGTGGCAAGCCCTGGAAGTGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAc                             <  2:174380/233‑1 (MQ=255)                                                                                                                                                                                          gACTACGACATGACGCAT‑ATTTTCA‑C‑GGGTACATTAGGGGAAGCCG‑ATCCTTGCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAc                             <  1:18066/239‑1 (MQ=255)                                                                                                                                                                                          gACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGt                                                                                                                                                                                                                                        <  2:396340/38‑1 (MQ=255)                                                                                                                                                                                               cGACTTGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGAATTCTATCCGGACCCCTAAGGTGCGCATTACGAAAAGGTCGGTGGGGACGGGCAAACCTAt                         >  1:818997/1‑238 (MQ=255)                                                                                                                                                                                                 ataTGGCGGAG‑GTTTGCG‑C‑GGGGACAGTAGGTGGAGCCG‑ATCCTGTCCACCCCGTGACGCTCGTTGCAAATCACGACACCCAACCGGTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTAGCCGGACCGCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCg                      <  2:909595/237‑1 (MQ=255)                                                                                                                                                                                                    tGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑At                                                                                                                                                                                                                             >  1:396340/1‑38 (MQ=255)                                                                                                                                                                                                             aTTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACctctc                                                                    >  1:987051/1‑181 (MQ=255)                                                                                                                                                                                                                         gTACATTAGTGGAAGCCG‑TTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGa  <  1:831211/238‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       TTTTATAAAGAGTGGATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTGGTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGATGAAATTCCAT‑GAAGCATCGCGCATGGGGCGC‑GACTACGACATGACGCAG‑ATTTTCA‑C‑GGGTACATTAGTGGAAGCCG‑ATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTG‑GCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGA  >  NC_000913/2006885‑2007333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑