Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,098,166 T→A 11.9% I46F (ATT→TTT)  wzzB ← regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,098,1660TA11.9% 207.3 / 3.2 74I46F (ATT→TTT) wzzBregulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base T (42/21);  new base A (3/7);  total (45/29)
Fisher's exact test for biased strand distribution p-value = 3.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGTTCGATGGTAAGTTTTTCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATA  >  NC_000913/2097932‑2098385
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             
aaGGTTCGATTGTAAGTTTTTCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGc                                                                                                                                                                                                                           <  2:255150/238‑1 (MQ=255)
aaGGTTCGATGGTAAGTTTTTCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGc                                                                                                                                                                                                                           >  1:255150/1‑238 (MQ=255)
aaGGTTCGATGGTAAGTTTTTCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGGGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACGGCCAAAAAACAAAAAGcc                                                                                                                                                                                                                          >  2:457413/1‑239 (MQ=255)
  ggTTCGATGGTAAGTTTTTCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAg                                                                                                                                                                                                                        >  1:649695/1‑239 (MQ=255)
     tcaaTGGTAAGTCTTTCTCGTTCTTCTTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTAAAGCGACCAATTAACTTCTCCTGCAAATCCGAAACTTTCGGTGCAGGCTATCCTTGGATAACATTCATAGCATTGTTTTAGCCAGCCATTTGCCCCCCGGCGGCCTGCGTGAAAACTTCTAGTTGCGTACACTTTTCCTTCGCTACTGCCAAATCTCCAAAAGCCAcgga                                                                                                                                                                                                                     >  2:851078/4‑238 (MQ=255)
     tCGATGGTAAGTTTTTCTCGTTCTTCCTGATTATACAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTAACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAACCAGGGc                                                                                                                                                                                                                     >  1:393124/1‑239 (MQ=255)
             aaGTTTTTCTCGTTCTTCCTGATTCTCCAGCGCTTTTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGCCTGAGTGATAATTGCTTTTGACTTCATTTTTCCCTACGCTACTCGCAAAAATCAAAAAGc                                                                                                                                                                                                                           >  2:142571/1‑225 (MQ=255)
                   ttttcGTTCTTCCTTATTACCCAGCTTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGa                                                                                                                                                                                                      <  2:911404/236‑1 (MQ=255)
                   tttcggTTTTTCCTGGTTATCCCGGTTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCGGCAAAGCCGAAACTTTCGGTGCAGCCTGACCAGAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTGCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGa                                                                                                                                                                                                      <  2:807811/234‑1 (MQ=255)
                   ttCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATTCTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGa                                                                                                                                                                                                      <  2:191154/239‑1 (MQ=255)
                   ttCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTAATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCAAGGGCAATAGCCAAACTGa                                                                                                                                                                                                      >  1:247657/1‑239 (MQ=255)
                                    tATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTc                                                                                                                                                                                     >  1:676089/1‑239 (MQ=255)
                                            gTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTTTCATCTTgc                                                                                                                                                                              >  1:756683/1‑238 (MQ=255)
                                            gTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCATTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCAACTTgcc                                                                                                                                                                             >  1:472974/1‑239 (MQ=255)
                                                    gaaTGCAGAGAAGGCAGAACAGAAGGCACCAGTTAACGTCGCATGAAAGTCCAATGCTTGCGTGGAAGCCTAACCAAAGATAACATGCATGTCATTGTTAGACACAGAAATTGGCCCCACGGCGGGCTAAGTGAGATTTGCTGTGGACGTCCATTTCTCCTGCGTCACTACCAAATCACCAATAGCCAGGGCAATAGCCACAATGACGGAAATAATGATTGTCTTTTGcccacgccac                                                                                                                                                                      <  1:41433/237‑1 (MQ=255)
                                                     aatgcaagaaaGGCAAACTGAAAGCCAACAATAAACGTCCCCTGAAAATCGTATACTTTGGGTGCAGCCTGCCCATAGAAAACATTCATGGCATTGTTATGGCCAGCATTTTGCCCCACGTCGGGCTGAGTAATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCAAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACaa                                                                                                                                                                    <  1:471856/230‑1 (MQ=255)
                                                       tGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCcaca                                                                                                                                                                     >  1:1086989/1‑236 (MQ=255)
                                                       tGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACaa                                                                                                                                                                    >  1:959614/1‑237 (MQ=255)
                                                             gaAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATTACGGAAATGATAATTGTCATCTTGCCACCCCAAAAATGCAcaa                                                                                                                                                            >  2:8656/1‑237 (MQ=255)
                                                             gaAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTa                                                                                                                                                            >  1:1077938/1‑239 (MQ=255)
                                                             gaAGGCAGAACTGAAGCCACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGCGTGATAATTTCTGTTGACGTCCATTTCTCCTTCCCTACGGCCAAATATCCAATAGCCCGGTCAATAGCCACAATGACGGAAATGATGATTTCCATTTTccaacgccaaacccacccaa                                                                                                                                                            >  2:884425/1‑228 (MQ=255)
                                                                            atcGACAAATTAGCGTCTCCGGCAAATCCGATCCTTTCGGGTCAGCCTGACAATAGATAACATTCATGGATTGGTTAAACCCAGCATTTGCCCCCACTGCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCCCCTTCGCTACTGCCAAATATCCAAAAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaa                                                                                                                                              <  1:216140/237‑1 (MQ=255)
                                                                            aGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGAGGATTGCCTTCTTTCCCCGACCCAACACAACTAGtaaacaaacaaaa                                                                                                                                             >  2:747732/1‑238 (MQ=255)
                                                                                aCCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATACCATTCATGGCATTGTTCTAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGTGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACTCCACAACTGCACTAGTaaatcaatcaaatcaa                                                                                                                                          >  1:424351/1‑239 (MQ=255)
                                                                                aCCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGCCATCTTGCCACGCCCCAACTGCACTAGTaaatcaatcaaataaa                                                                                                                                          >  1:162296/1‑239 (MQ=255)
                                                                                 ccAATTAACGTTTCCTGAAAATCCGATTTTTGCGGTGCAGCCTACCCAAAGAAAACATTCATGGTTTTGTTAAAGCCAGAAATTTGCCCCAGGTCGGGCCGGGTTATAATTGGTGTGGACGTCCATTTCTCCTCCGCTACTGCAAAATATCCAATAGCAAGGGCAATAGCCACAATGACGGAAATTATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaatcaat                                                                                                                                         <  1:140815/239‑1 (MQ=255)
                                                                                                   aaaCCCGAGACTTGCGGGTCAGCCTGACCATAGAAAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCGGAGTTATAATGGCTGTTGACGTCCTTTTCTCCTTCGCTACTGCAAAATATCCAATACCCAGGGCAATAGCCAAAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGtt                                                                                                                       <  1:624360/239‑1 (MQ=255)
                                                                                                            ttttggggTGCGGCCTGACCATAGATAACTTTCTGTGCTTTGTTAGAGCCAGCAATGTGCCCCCTGGCGGGTGTAGGAATAATTGCTGTTGCCGCCCATTTCTCCTTCGCTTCTGCAAAATATCCAATAGCCGGGGAAATAGCCACAATGACGGAAATGATGATTTCCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGa                                                                                                              <  2:89309/233‑1 (MQ=255)
                                                                                                                  ggTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCa                                                                                                                                                               >  1:799976/1‑184 (MQ=255)
                                                                                                                      cacccggcccaaaaaTAACTTTCAGGGCATTGTTAAAGCCAGCAATTTCCCCCACGGTGGGTTGAGGAATAATCTCTGTTGACGCCAATTTCCCCTTCGCTTCGGCCAAATTCCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACattatt                                                                                                     <  1:187287/224‑1 (MQ=255)
                                                                                                                          tttataagacaccgcttttgtGGTATTTTTAGACCCAGCATTTTCCCCCGGTTGGGGCAGAGTAATAAGTGCTGTTGACGTCCATTCCCCCTCCGCAACGCCAAAATACCCAAAAGCCGGGCCAAAAGCCACAATGACTGAAATGATGATTGTCATCTGCCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTAtttt                                                                                                <  2:344013/219‑1 (MQ=255)
                                                                                                                          ttgacatcagacacgtttCAGGGCATTTTTACGCGCAGAAATTTGCCCCACGCCGGGCTGAGTAATAATTGGTGTCAGAGTCCATTTCGTCTGCGCTCCTCTCAAATATCCAATAGCCAGGACAATAGCCAAAGTGACGGAAATGATTATTTTCATCTGGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTAtttt                                                                                                <  1:1006833/223‑1 (MQ=255)
                                                                                                                               cATAGTTAACTTTCGTGGATTTGTTAAAGCCGACAATTGTCCCCACGTCGGACTGGTTGATATTTGCTGTTGACGCCCATTTCACCTTCGCTCCTGCCAAATATCCAATGGCCAGGCAAATAGCCAAAATGAAGAAAATGATGATTGTCATCTTGCCCCGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTAct                                                                                           <  1:949185/239‑1 (MQ=255)
                                                                                                                                 aaaaTAACATTCATGGCTTTTTAATAGCCAGAAATTCCCCCCGGGTCGGGCTGAGTGATAATTGTTGTTGACTTCCATTTCCCCGTGGCTATTGCCAAATATCCAATAGCCAGGGAAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActct                                                                                         <  1:8656/236‑1 (MQ=255)
                                                                                                                                       aCATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTcttcat                                                                                   >  1:623120/1‑239 (MQ=255)
                                                                                                                                             aTGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCt                                                                             >  1:539697/1‑239 (MQ=255)
                                                                                                                                               ggCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCAt                                                                                                                                                                                   >  2:334544/1‑135 (MQ=255)
                                                                                                                                               ggCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCAt                                                                                                                                                                                   <  1:334544/135‑1 (MQ=255)
                                                                                                                                                   ttGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcat                                                                                      >  1:344013/1‑224 (MQ=255)
                                                                                                                                                    tGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTc                                                                                                                                   >  1:434441/1‑178 (MQ=255)
                                                                                                                                                           gCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAACTCAATCTGTTCCGGGCCATGGTTTTGCCCAGACCAATTATTATTTTCACCTCTCTTCATTACCCTAAAGCACGGAAAtt                                                               >  2:591408/1‑239 (MQ=255)
                                                                                                                                                           gCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTAACGGAAAtt                                                               >  1:532795/1‑239 (MQ=255)
                                                                                                                                                           gCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTAACGGAAAtt                                                               >  2:712922/1‑239 (MQ=255)
                                                                                                                                                              aGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTcc                                                            >  1:978601/1‑239 (MQ=255)
                                                                                                                                                                cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCCCAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCCGGGTTTGTCCCAGAAACATTATAATTTTTTACTCTCATCATTACCCTAACTGCAGGAAATTTCaaa                                                          >  2:288008/1‑239 (MQ=255)
                                                                                                                                                                cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCaa                                                          >  1:555257/1‑239 (MQ=255)
                                                                                                                                                                    tggcccccgggcGGGCTGGGGTATAATTGCTTTTGACGTCCATTTCTCCTTCCTTACGGCCAAATTCCAAAAGCCCAGGGAAATAGCCAAAATGACGGAAATGATGATTTTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGt                                                        <  1:591408/226‑1 (MQ=255)
                                                                                                                                                                      gcccccggcggggctggtgtaTAATTGCTGTTGACGTCCATTTCTCCTGCGCTACGGCCAAATACCCAAAACCCAGGGAAATAGCCAAAAGGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCa                                                   <  1:716687/219‑1 (MQ=255)
                                                                                                                                                                       ccccccGTCGGGCTGATTTATATTTGCTGGTGACGTCCTTTCCTCCTTCGCTACGGCCAATTACCAAATAGCCAGGGAAATCGCCACAATGACGGAAATGAGTATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCa                                                   <  2:1077938/239‑1 (MQ=255)
                                                                                                                                                                           aCGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAAGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGCCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCCTTACCCTAACTGACGGAAATTTCCaaacccccaaagt                                               >  2:397838/1‑228 (MQ=255)
                                                                                                                                                                                 ggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTTACGGAAATTTCAAAGTCCCCAAAGTTTATCg                                         >  2:28330/1‑239 (MQ=255)
                                                                                                                                                                                   ctggtgaatatTTGTTGTGCACGTCCATTTCCCTTTCCTACTGGCAAAATTCCCAATAGCCAGGGCAATAGCCAAAAGGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGa                                        <  1:457413/231‑1 (MQ=255)
                                                                                                                                                                                            atttGCTGTTGACGCCAATTTCCCTTTCGCTACTGCAAAATTGCAAATAGCCAGGGCAATACCCACAATGACGGAAATGTTTATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGt                              <  1:462514/237‑1 (MQ=255)
                                                                                                                                                                                            aaTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGt                              >  1:318311/1‑239 (MQ=255)
                                                                                                                                                                                             aattcgttttGACTTCCATTCCCCCTTCGCGTCTGCAAAATACCCAATAGCCAGGGAAATAGCCAAAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGt                              <  1:57065/231‑1 (MQ=255)
                                                                                                                                                                                               tGCTGTTGACGTCCTTTTCCACTTCGCTACTGCAAATTTTCAAAAGGCCAGGCAAATGACCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa                           <  1:747732/239‑1 (MQ=255)
                                                                                                                                                                                                tttttttgcgcctctttttcccttccttACTGACAAATATCCAATAGCAGGGGAAATAACCAAAATGAAGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa                           <  2:1086989/219‑1 (MQ=255)
                                                                                                                                                                                                gCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaat                                                                                                                                                  >  1:959868/1‑119 (MQ=255)
                                                                                                                                                                                                gCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTCCTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTACCGAAAAACCTccggaaa                          >  2:547825/1‑233 (MQ=255)
                                                                                                                                                                                                gCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAAt                          >  1:72788/1‑239 (MQ=255)
                                                                                                                                                                                                      tgccGTCCTGTTCCCCTCCGCTACGCCCAATATCCAAATAGCCGGGCAAATGGCCAAATTGACGGAAATGATATTTGTCATCTGGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCta                      <  1:884425/235‑1 (MQ=255)
                                                                                                                                                                                                       gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACattatt                                                                                                     >  1:837479/1‑158 (MQ=255)
                                                                                                                                                                                                       gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGCCATgg                                                                                                                         >  1:38673/1‑138 (MQ=255)
                                                                                                                                                                                                          gTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACatta                                                                                                       >  2:827628/1‑153 (MQ=255)
                                                                                                                                                                                                          gTCCATTTCTCCTTCGCTACTGCCAAATATCCAAGAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACatta                                                                                                       <  1:827628/153‑1 (MQ=255)
                                                                                                                                                                                                             cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATaa              >  1:179898/1‑239 (MQ=255)
                                                                                                                                                                                                               aatttcccctgccttaaggcAAAAATCCAAAAAGCCGGGGAAAAAGCCACAATGAGGAAAATGATGATTGCCATTGTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTGCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATaa              <  1:989088/220‑1 (MQ=255)
                                                                                                                                                                                                                  ctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGAt         >  1:575898/1‑239 (MQ=255)
                                                                                                                                                                                                                        cGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGa                 >  1:963293/1‑225 (MQ=255)
                                                                                                                                                                                                                         gCTACTACAAAATTTCAAAAACCAGGGCAAATAGCCAAAATAAAGAAAATTGTGTTTGCCATCTGGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAaata  <  2:318311/239‑1 (MQ=255)
                                                                                                                                                                                                                                   aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTc                                                             >  2:596606/1‑171 (MQ=255)
                                                                                                                                                                                                                                   aaaTATCCAATAGCCAGGGAAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTc                                                             <  1:596606/171‑1 (MQ=255)
                                                                                                                                                                                                                                      tatCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGtt                                              >  1:412883/1‑183 (MQ=255)
                                                                                                                                                                                                                                            aaTAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAggc                 >  1:813272/1‑206 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             
AAGGTTCGATGGTAAGTTTTTCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAACGTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATA  >  NC_000913/2097932‑2098385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: