| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,126,971 | T→G | 14.1% | T74P (ACC→CCC) | wcaG ← | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,126,971 | 0 | T | G | 14.1% | 186.8 / 4.1 | 77 | T74P (ACC→CCC) | wcaG | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Reads supporting (aligned to +/- strand): ref base T (28/33); new base G (12/3); total (41/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCGTTGTTTACTCATG > NC_000913/2126749‑2127191 | cGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCACCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTTCCCGTTTCCGGTAGATGCAGGACGATCCGAGAAACC‑GCCGTTTGTTCACGTCGGTCTGATGCCCCGCGTGAATGATGTTGCTCCCAATCATCATTTTTCTGTAAGA‑‑GAAATCCCCCGGATA‑GGGGGTGTTTGcaacaaa > 2:251350/1‑237 (MQ=255) aagaggTTTGATCCCGGCTATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCTTGCAACAACTCGCGTTCTGCCATCGGCTGGTTTGCCAGTTGCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGGGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATg < 1:680051/233‑1 (MQ=255) cGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCTGCGTGAATGATGTTGCTCTCAATCCTCC‑TGTTCTGGTATA‑TGAAATACGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgc > 1:381220/1‑238 (MQ=255) gCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCCGTTTCCGGTAGATGCAGGGCGATCCGCGCAACA‑GCAGTTTGTTTACCTCGTTCTGATGCGCGCGGGGAATGAAGTTACCCTCAATC‑TCACTGTTTTGGTGGG‑TGAAATGTCCCGGGTA‑GGGGTTGTTGCAAACAAACCACCCCACTTTtgcccc > 2:1032691/1‑237 (MQ=255) gCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTTATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGG‑TGAAATCCGCCGGATA‑GGGGTTGTTGGCAACAATGCCGCCCACTTTcgccg > 1:453224/1‑238 (MQ=255) gCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTTTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGTTA‑GGGGTTGTTGGCAACAATGCGGCCCCCTTTcgccg > 1:827323/1‑238 (MQ=255) ttataagagccagggCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCCGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgccgcc < 1:1021371/225‑1 (MQ=255) cggttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgccgc < 1:923265/235‑1 (MQ=255) ttttttGGCAATAGCATTAGGTTCGTTAGTGGGTTCCAGCGTGCCCTGCAACAACTCTCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgccgccg < 2:960844/237‑1 (MQ=255) atgttgataagagacagaGGCTCGGTAGTCGGCTTCAGCGGGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgccgccg < 1:698803/221‑1 (MQ=255) ttgtaaTGGCATTAGGCTGGTTTGTCGGGGCCAGCGTGCCCTGCAACAACTCGGTTTCTGCCATCGGCTGTTTTGCCCGTTGCGGGGAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGa < 2:827323/235‑1 (MQ=255) tGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGa < 1:764216/239‑1 (MQ=255) tagtctAATGCTCGTTTGGCGGCCCCCGCGGTCCCTGCAACAACCCGTTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGAcc < 2:796881/234‑1 (MQ=255) aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGGTTCGGGTAGATGCAGGCCGATCCCAGAAACA‑GCGCTTTGTTCACGCCGTTCTTAGGAGCGGGGTGAATGTAGTTGCTCTCCATCATTA‑TGGTCTGGCAGAGTGAAA‑CCGCCGGCTG‑AGGGTCGATGTCAACAAAAACGCCCACtttaccccccccccgacaaaccc > 2:626804/1‑219 (MQ=255) aGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCCCCGGATA‑GGTGTTGTTGGCAACAATGCCCCCCCCTTTCGCCCCCCCCCGATAGACCTGGTCaa > 1:238390/1‑239 (MQ=255) cGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGGAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTTACGTCGTTCTTGTGCGGGGCGTGAATGAGGTTTCTCTTGATCCTCA‑TGTTCTGGTAGA‑TGAAATACCCCCGGTA‑GGGGTTGTTGGCAACAAATGCGCCCACTTTCCGCGCCCCCCGCAAGACCGGGtacaaagat > 2:661578/1‑231 (MQ=255) gTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGTCAGTTTCGGGTAGATGAAGAACGAACCGTGAAAAA‑GCAGGTTGTTCACGTCGTTCTGATGGGCGGCGCGAATGATGTTGCTCTCAATCATAA‑TGTTATTGAAGA‑TGAGATCCGCCGGAAA‑GACATTGTTGGCAACAAAGCCGCCCACTATCGCCGCCGCCAGAGAGACCTGGTcctggctct > 1:110171/1‑231 (MQ=255) gTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATTCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTTGTAGA‑TGAAATCCGCCGGAAA‑GGTGTTGTTGGCAACAATGCCGCCCCCTTTCGCCGCCGCCAGATAGACCTGGGCAATACGt > 1:559179/1‑238 (MQ=255) tAGTCGGCTCCAGCGGGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCg < 1:879759/239‑1 (MQ=255) tCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGGTTTTCCAGTTTCGGGTAGAAGCAGGACGATCCGAGAAACA‑GCCGTTTGTTTCCCTCGGTCTGATGGGCGGCGGGAAGGAAGTTTCCTCCAATCATCA‑TTTTCTGGTAGA‑TGGAATACCCCGGAAAGGGTGTTGTTGCAAAAAAAGCC‑CCCCCTCTTCCCCCCGCCCAATAGAcccccgttcttatacacat > 2:698803/1‑221 (MQ=255) tCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCCGATAGACCTGGTCAATACGTTCGCTGGCaaa > 1:1021856/1‑239 (MQ=255) ccATCGGGCCCTGCAACAACTCGCTTTCGGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATTCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAg < 1:591249/239‑1 (MQ=255) ccAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAg < 2:1021856/239‑1 (MQ=255) cccTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGGGTTGTTGGCAACAAAGCCGCCCACTTTCCCCGCCGCCAGATAGACCTGGGCACTACGTTCGCCGGCAAAGAAATCAt > 1:441820/1‑238 (MQ=255) cccTGCAACAACTCGCTTTCTGCCATCGGCTGCTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCCCGTCGTTCTGATGCGCGGCGGGAATGATCTTGGTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGGGTTGTTGGCAACAATGCCGCCAACTTTCGCCGCCCCCAGTTAGACCTGGTTCATCCGCTCGCTGGCAAAGAAATCAt > 1:740223/1‑238 (MQ=255) caacaaCTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACgg < 1:463826/239‑1 (MQ=255) acTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCCGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACCGcccg > 1:966828/1‑238 (MQ=255) acTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGGGTTGTTGGAAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCAGTACCGGCGCgg > 1:110300/1‑239 (MQ=255) cTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGGAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGGGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGt > 1:984485/1‑200 (MQ=255) cTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgc < 2:543886/180‑1 (MQ=255) cTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgc > 1:543886/1‑180 (MQ=255) cGCTTTCTGCCATCGGCTGTTTTGCCAGGTTTGGGAAGATGTAGCAGGATCCCTGAAACA‑GCCGCTTTTTCACGGCGGTCTGAAGCGCCGCGGGAATGATGTTGCTCTCACCACTCA‑TGATCTTGGGGA‑TGAAAACCGCCGGGTA‑GGGGTTGTTCGCAACCAAGCCCCCCCCTTTCCCCCCCCCCCGATAGGCATGGTCAATACGTACCCCGCCCAAAAACACATGCACgggcggggtg > 2:581863/1‑231 (MQ=255) gttttCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAg < 1:818082/215‑1 (MQ=255) cTGCCATCGGCTTTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCcagc < 1:661578/239‑1 (MQ=255) cTGCCATCGGCTTGTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCcagc < 1:956519/239‑1 (MQ=255) aTCGGCTGTTTTGCCAGTTTCGGGTAGAAGCAGGGAGAACCGGGAAACA‑GCAGTTTGTTCACGTCGGTCTGATGCGCGGCGGGAATGATGTTGCTCTCAATCATCA‑TGTTTTGGGAGA‑TGAAATCTGCCGGATA‑GGGGTTGTTGGCCACAATGGCGCCCACTTTTCCCGGCGCCACATCGAACAGGTCAATAAGTTCGCTGGCACAAAAAACATGCAccggtctcttttacaaaactga > 2:818082/1‑218 (MQ=255) ccAGTTTTGGGTTGATGGGGGACGGTCCGAGAAACT‑GGAGTTTGTTCACGTCGTTCTGATGCGGGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCgg < 2:1078271/239‑1 (MQ=255) ccAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGGCAATACGTTCGCTGGCAAAAAAATCATGGACGGCCGGGCTGTCCAGCAGGTTCAGCtcgcgccgg > 1:692194/1‑233 (MQ=255) tCGGGTGGATGCAGGCCGTTCCGGGAAACG‑GCAGTTTGTTCACGTGGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGt < 2:453224/239‑1 (MQ=255) aggtGCAGGGCGATCCGAGAAACC‑GCAGTTTGGTCACGTCGTTCTGATGCGCGGCGGTAATGATGTTGCTCCCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATAc < 1:950202/235‑1 (MQ=255) aTGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTTGCAACAATGCCGCCCACTTTctc > 2:625284/1‑138 (MQ=255) aTGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTcgc < 1:625284/140‑1 (MQ=255) aTGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGCTAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAg > 1:1024258/1‑239 (MQ=255) gACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGGGCGTAATACCAGTTCcac > 1:926057/1‑239 (MQ=255) gACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGCCAATACGTTCGCTGGCAAAACAATCATGCACGCCCCGGCTGTCCACCCGGTTCAGCTCCTCCCGGGTGCGAAAACCCAGTTCcac > 2:842418/1‑239 (MQ=255) gagaAACA‑GCAGTTTGGTCACGTCGTTCGGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACcgc < 1:570521/239‑1 (MQ=255) gaAACAGGCGGTTTG‑TCACGGCCTTCTGATGCGCGGCGTGAATGATTGTGCTCTCAATCATCA‑TGTTCTGGTTGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACcgcg < 1:1027418/238‑1 (MQ=255) gaAACA‑GCAGTTTGTTCCCGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACcgcg < 1:251350/238‑1 (MQ=255) tttGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGa > 1:633342/1‑236 (MQ=255) ttGTTCACGGCGTTCTGATGCGCGGCGTGAAGGATGTTGCTCTGAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAg < 1:789163/236‑1 (MQ=255) tCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGGTTTGTTGGCAACAATGCCGCCCACTTTCGCCCCCGCCAGATAGACCTGGTCAAGACGTTCGCTGGCAAAGAAATCATGCACGGGACGGCGGTCCAGCAGTTTCAGCTCTTCGCGGGTGCGCAAAACCAGTTCGACACCCCCGGGCTGTTccgacggccc > 2:810104/1‑229 (MQ=255) gtcgtTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACCTCACCGCCCTGTTCGAGCTGCCGCCTGa > 1:51330/1‑239 (MQ=255) ctcggcgTGAATGTTTTTTCCCTCAATCACCT‑TGTTCTGGTTGA‑TGAAATCCGCCGGATA‑GGTGTTGTCGGCAACAATGCCGCCCCCTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGa < 2:968809/237‑1 (MQ=255) gcggcgTGAATGATGTTGCCCGCAATCCACA‑TGTTCCGGTAGA‑GGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGa < 1:581863/238‑1 (MQ=255) cggcgTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGAcc > 1:968809/1‑239 (MQ=255) gcggtaaTGATGGTGTTTTTAATCATCA‑TGTTCTGGTAGA‑GGAAAGCCGCCCGATG‑GGGTTTGTTGGCAACAATGCCGCCCCCTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGGCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCAt < 2:238390/234‑1 (MQ=255) gttaagtgtgTTGCTGTTAATCAACA‑TGGTTCGGTAGA‑TGAAAACCGGCGGATT‑GGGGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGACAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATc < 2:51330/230‑1 (MQ=255) gTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCTTCGCGGGGGCGTAATACCAGTCCCACATCCCCCCGCCGTTCGGCCCGCCGCCCGATGGCGGACCCGccccccccccacgacc > 2:830869/1‑226 (MQ=255) ggtatcaattcaca‑tGTTCTGGTATA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAAGGCCGCCCGCTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAg < 1:428844/227‑1 (MQ=255) gccccaaaTCGTCA‑TGTTCTGGTAGA‑TGGAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCTGATAGACCTTGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGGTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAg < 1:626804/233‑1 (MQ=255) agtctcAATCATCA‑TGGTCGGGTAGA‑TGAAATCCGGCGGAAG‑GGTTGTGGTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGGTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAg < 2:990262/237‑1 (MQ=255) ctctCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGCCCCACCCCCGATGACCAGc > 1:840526/1‑239 (MQ=255) ctctCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCCCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGACCTGCCGCCTGATGGCGGAACCGACCATCCCCCGATGCCCAGc > 1:6722/1‑239 (MQ=255) ctctCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCGGTCCAGCAGGTTCAGCCCGTGCCGGGTGCGTAATACACGGTCCACATCACCGCGCCGTCCAGGCGGCCGCCTGCTGGGGGACCCgccacaccccccctgcaccgc > 2:1102067/1‑219 (MQ=255) aaTCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGCCCTGGTCAATACGTTCGCTGGCAAAGAAATCATCCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGGGCGCAATACCAGTTCCCCCTCCCCGCGCTGTTCGAGCTGAGTCCTCATGGCGGAACCCACCCTCCCCCGATgccacaccaaaa > 2:904046/1‑228 (MQ=255) aaTCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCggg < 2:1063116/155‑1 (MQ=255) aaTCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCggg > 1:1063116/1‑155 (MQ=255) a‑tGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCCCGATGACCAGCAATAAAAAc > 1:990262/1‑236 (MQ=255) ttCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCgttg > 1:956819/1‑239 (MQ=255) ttCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCCCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCACCCCCCGACAACCAGCAAAAAAAAACCgttg > 2:1013724/1‑239 (MQ=255) gTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGGGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCGGATGGCGGCACCGACCATCCCCCGAGGACCACGAATAAAAACCCGTTGtttc > 2:689108/1‑237 (MQ=255) gTAGA‑TGAAATCCGCCGGATA‑GGGGTTTGTGGCACCAATGCCGCCCCCTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCGTTGTTTAc < 2:110346/239‑1 (MQ=255) tAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCgg < 1:184964/138‑1 (MQ=255) tAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCgg > 2:184964/1‑138 (MQ=255) aaa‑TGAAATCCGCCGTGTG‑GGTGTTGGTGGCAACAATGCCCCCCACTTTCGCCGCCGCCAGATAGACCTGGGCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCGTTGTTTACt < 2:956819/236‑1 (MQ=255) gA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCGTTGTTTACTc < 1:689108/238‑1 (MQ=255) tGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCGTTGTTTACTCATg > 1:110346/1‑239 (MQ=255) tgttggTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATaaa < 1:908533/202‑1 (MQ=255) | CGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCTGTTTTGCCAGTTTCGGGTAGATGCAGGACGATCCGAGAAACA‑GCAGTTTGTTCACGTCGTTCTGATGCGCGGCGTGAATGATGTTGCTCTCAATCATCA‑TGTTCTGGTAGA‑TGAAATCCGCCGGATA‑GGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCATGCACGGCGCGGCTGTCCAGCAGGTTCAGCTCGTCGCGGGTGCGTAATACCAGTTCCACATCACCGCGCTGTTCGAGCTGCCGCCTGATGGCGGAACCGACCATCCCGCGATGACCAGCAATAAAAACTCGTTGTTTACTCATG > NC_000913/2126749‑2127191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |