Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,490,509 T→C 17.7% G480G (GGA→GGG oxc ← oxalyl CoA decarboxylase, ThDP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,490,5090TC17.7% 121.9 / 2.2 62G480G (GGA→GGGoxcoxalyl CoA decarboxylase, ThDP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base C (3/9);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCA  >  NC_000913/2490287‑2490746
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              
ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCACCGCCCCTGAGATCAAAACCGTCTCCTCTCTAGATgcagc                                                                                                                                                                                                                                >  1:397656/1‑239 (MQ=255)
       tGGCCAGTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGGCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCAACTGTCGTGGTGACGTTATGGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCattatt                                                                                                                                                                                                                         <  1:233526/239‑1 (MQ=255)
       tGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCCCGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGCTGGTGATGGTGCACCAGCGCCACTGAGATCAACAACACCCCCTCTGAAGATGCCGCCaataat                                                                                                                                                                                                                         >  2:337087/1‑237 (MQ=255)
       tGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTTTCATACCTTGCATGGTGCAACAAATCCGTTGGTGCTGGTGCACCAGCGCCACGGAGATAAAACACTGtctcttataaaaaatggaacccgcc                                                                                                                                                                                                                          >  2:371459/1‑218 (MQ=255)
              tttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGATGGTGATGGGGCACAAGCGCCACTGAGATCAACACCGTCTCATCTGTAGATGCCGCCATTATTAAAAATa                                                                                                                                                                                                                  >  1:469605/1‑239 (MQ=255)
                                            caTTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCCACaa                                                                                                                                                                                    >  1:677493/1‑239 (MQ=255)
                                            caTTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGAACCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCCCCTCTGTAGATGCCCCCATTATTAAAAATAAAGAACGTCACCGGCAGGTTTAATCGCCaa                                                                                                                                                                                    >  2:1016701/1‑239 (MQ=255)
                                                    atGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCTTACCTTGCATGGTGCACCAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGGAGATTCCCACATTATTACAAATAAAGCTCCTCACCGGCAGGTTAATTCGCAAAAACGttta                                                                                                                                                                            >  2:883263/1‑238 (MQ=255)
                                                     tGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGGGATGGTGCACCATCGCCACTGAGATCAAAACCGTCCCCCCTGTAGATGCCGCCATTATTAAAAATAACGAACGCCACCGGCAGGTTAAATCGACAAAACGtttaa                                                                                                                                                                           >  2:216745/1‑237 (MQ=255)
                                                       gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGa                                                                                                                                                                                                              <  1:248979/202‑1 (MQ=255)
                                                       gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGa                                                                                                                                                                                                              >  2:248979/1‑202 (MQ=255)
                                                       gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCAGGGTGCAACAGATCCGTTGGTGATGGTTCACCAGCGCAACTGAGATCCAAACCGTCTCCTCTGGAAGTGCCGCCATTATTAAAAATAACCATCGTCACCGCCAGGTTATTTCCACaaaaccgtttaat                                                                                                                                                                         >  2:969754/1‑229 (MQ=255)
                                                           gTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCACCGCCAATGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGACCCTCCCCGGCAGGGTATCTCGCAAAAACGTTTCAAtttac                                                                                                                                                                     >  2:850698/1‑237 (MQ=255)
                                                                     cTGAATCCCGGGGGTTAAAGCATGACGAAGCTCATCTGTCGTGGGTACGGTAAGGCCAACGCCACGAAACGCATCCATTAAGTTGTCATACCTTGCATGGTGCAACAGATCCTGTGGTGATGGTTCACCAGCGCCACTGAGATCAACACCTGCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg                                                                                                                                                            <  1:127530/238‑1 (MQ=255)
                                                                               gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCACCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAAAAACAACCGTCACCGACAGTTTATATCCACAAACCGTTTCAATTTCCATCCCACGCAACCCaaaac                                                                                                                                                  >  1:205351/1‑237 (MQ=255)
                                                                                  gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGCCACCGCCAGGTTTTATCGCCAAATCATTTCAATTTACATCCCACTAAAACCAAAAGAAca                                                                                                                                              >  2:434415/1‑238 (MQ=255)
                                                                                     aaaGCCAGACGGAGTTCATCTGTCGTAGTGACGTTATAGCCAACGCCACGAAACGCATCCATGAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTTACCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATc                                                                                                                                           <  1:375268/239‑1 (MQ=255)
                                                                                            gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGAGGCCGCCATTATTAAAAATAACGAACGTCACCGGCCGGTTTAATCGCCAAACACTTTCAATTTCCATCCCCATAAAACAaaaaaaacaaaaacctaaa                                                                                                                                    >  2:883035/1‑224 (MQ=255)
                                                                                                           gcggtgttaCGTTATAGCCCACCCCCCGAAACGGATCCATTAATTTGTCAATCCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGga                                                                                                                     <  1:334055/231‑1 (MQ=255)
                                                                                                                       tataGCAAACTCCACGAAACGCCTCCATTAATTTGTCATTCCTTGCATGGTGCAACAGATCCGTTTGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGt                                                                                                          <  1:337087/238‑1 (MQ=255)
                                                                                                                               aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAGCAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAACCGTTTCAATTTCCATCCCACTAAAACACAAAACCCCATCACCCTCACTGGCGACAAcccggaaaacaaaaaaacccaccaa                                                                                                 >  2:1061037/1‑216 (MQ=255)
                                                                                                                               aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGga                                                                                                                     >  1:617303/1‑219 (MQ=255)
                                                                                                                                cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGCCAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTAAATGGCGACAACCGGCGACACAGAAGTCCCGccaaa                                                                                                 >  1:829607/1‑234 (MQ=255)
                                                                                                                                   cACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCg                                                                                             <  1:216745/239‑1 (MQ=255)
                                                                                                                                    aCGAACCGCGTGCATTAATTTTGCATCCCTGCCAGGGTGCAACGAGTCCGTTGGGGCAGCGCCCCCACCGCCACCGAGGTCAACCCCGTCCCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGa                                                                                            <  2:829607/239‑1 (MQ=255)
                                                                                                                                         aCGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                       <  1:1016701/239‑1 (MQ=255)
                                                                                                                                          cGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGCCGACAACCGGAAAACCAGAAGTCACGCCAGCACCGATGGCac                                                                                      >  1:937465/1‑238 (MQ=255)
                                                                                                                                                            aTACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGCAACCAAAATCACTATCACCTTCAATGGCGACAACCGGGGAACAAGAAGTCAAGCATGAACCGATGGCATACCCAAAACCGCTGccaa                                                                    >  1:847731/1‑237 (MQ=255)
                                                                                                                                                                  tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGCGAACCACAAGGCAACCCAACCCCCAGGCCAAAACCCAAACCGACGCCCaaaaaa                                                               >  2:572658/1‑233 (MQ=255)
                                                                                                                                                                  tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCAACATCACCTTCAATGGCGACAACCGGTGAACCAAAAACCCCGCCAACCCCGATGGCAAAACCCaacaccgcacccaagaac                                                               >  2:1031380/1‑221 (MQ=255)
                                                                                                                                                                  tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTAAATTTCCGTCCAACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCCAAGCAAAAGCCCATACCGAAGCCCacaaca                                                               >  2:122604/1‑233 (MQ=255)
                                                                                                                                                                   gCATGGTTCAACAGTTCCGTTGGTGATGGTGCCCCAGCGCCACTGAGATCAACCCCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCGTCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            <  1:122604/239‑1 (MQ=255)
                                                                                                                                                                   gCATGGTGCAAAAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            <  1:572658/239‑1 (MQ=255)
                                                                                                                                                                   gCAGGGTGCAACGGTTCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACCCCGTCTCCTCTGTAGATGCCGCCATTAGTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            <  1:969754/239‑1 (MQ=255)
                                                                                                                                                                   aaagggaacgaatattCCGTTTGTGAGGGGTCCCCAGGCCCACTGAAAACAAACCCGTCCCCTTTGTAGATGCCGCCATTATTAAAAATAACGATCGTCAACGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTACAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            <  2:71768/224‑1 (MQ=255)
                                                                                                                                                                    cATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCg                                                                                             <  1:1010401/206‑1 (MQ=255)
                                                                                                                                                                    cATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCg                                                                                             >  2:1010401/1‑206 (MQ=255)
                                                                                                                                                                            aaCAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACCCCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTAc                                                    <  1:617455/238‑1 (MQ=255)
                                                                                                                                                                             aCAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCCCCCTAGCACCGATGACATAGCCCCAACCGATGCCCAAAAAAACCCAGGAAcac                                                  >  2:249658/1‑237 (MQ=255)
                                                                                                                                                                              cAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGtctc                                                                                                                                                                                                                                               >  1:154392/1‑50 (MQ=255)
                                                                                                                                                                              cAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGtctc                                                                                                                                                                                                                                               <  2:154392/50‑1 (MQ=255)
                                                                                                                                                                                   ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGCCAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCACACCCATGCCCCTTACACCCCCGGGACCCCAAAcc                                            >  2:982953/1‑239 (MQ=255)
                                                                                                                                                                                   ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCCCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGACCCACAAATCCCGCAACACCCCAAGGCATAGCCCATACCAAACCCCATGAAAACCCCGGTAcaaaaaaca                                            >  2:969195/1‑231 (MQ=255)
                                                                                                                                                                                   ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCCTGACACCCCCGGAACCACAATcc                                            >  1:246454/1‑239 (MQ=255)
                                                                                                                                                                                   ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGACGCCAACCGGAGAACCAAAAGACACGCTAGCACCAATGGCCAAACCCAAACCGACGCCCAAGACACCCCCGGGACCCCaaaac                                            >  2:135376/1‑236 (MQ=255)
                                                                                                                                                                                   ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATAACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCCATGCCCAGACCACCCCAGGGACCACAATcc                                            >  1:103436/1‑239 (MQ=255)
                                                                                                                                                                                   ccGTTGGTGATGATGCACCAGCGCCACTCAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCAATATCACCTTCAATAGCGACAACCGGAGAACCAGAAGACACCCTAGCACCGATGGCATAGCCCAAACCGAACACCATGAAACCCCAGGAACCaaaacac                                            >  2:208977/1‑236 (MQ=255)
                                                                                                                                                                                        ggTGCTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATTACCTTCAATGGCGTCAACCGGGGAACCAGAAGTACCGCTATCCCCGATGGCATAGCCATAACCGATGCCCATGACACCCCAGGTCCcacctgcctctta                                       >  1:71768/1‑229 (MQ=255)
                                                                                                                                                                                             tGGTGCACCAGCGCCCCTGAGATCAACACCGTCTCCTCTGGAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:982953/239‑1 (MQ=255)
                                                                                                                                                                                              ggggCACCAGCGCCCATGAGATCAACACCGGTCCCTCTGTAGATGCCGCCATTAATAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:883263/235‑1 (MQ=255)
                                                                                                                                                                                              ggTTCACCATCGCCACTGAGATCAACTCCGTCTCCTCTGTAACTGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:434415/238‑1 (MQ=255)
                                                                                                                                                                                              ggTGCCCCAGCGCCACTGAGCTCAACACCGTCCCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:883035/238‑1 (MQ=255)
                                                                                                                                                                                              ggTGCACCAGCGGCACTGAGGACAACACCGGCCCCTCTGTAGATTCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:459506/238‑1 (MQ=255)
                                                                                                                                                                                                   aCCAGGGCCCCTGAGACCAACACCGTCTCCTCTGTAGATGCCGACATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGt                             <  1:208977/238‑1 (MQ=255)
                                                                                                                                                                                                       ccgcCACTAAAATCCACCCCCCCTCCCTTGTAGATGCCGCCATTAATAAAAATACCGATCGTAACCGGCAGGTTATATCGACAAAACGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTtata                        <  2:937465/238‑1 (MQ=255)
                                                                                                                                                                                                           ccacataagacAACCCCGTCCCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATAACGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTGCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACat                     <  1:850698/229‑1 (MQ=255)
                                                                                                                                                                                                            aCTGAGATCAACTCCGTCCCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACatat                   <  1:969195/239‑1 (MQ=255)
                                                                                                                                                                                                            aCTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGGCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACatat                   <  1:249658/239‑1 (MQ=255)
                                                                                                                                                                                                             ttaaaagagtcacccGTCTCCTTTTTTGATCCCGCCATTTTTAAAAATACCGACCGTCACCGGCAGGTTATAACGACAAATCGGTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACatat                   <  2:88613/226‑1 (MQ=255)
                                                                                                                                                                                                             tgtataagaggcagcGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACatat                   <  1:812238/225‑1 (MQ=255)
                                                                                                                                                                                                                  aTCAACCCCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:537718/218‑1 (MQ=255)
                                                                                                                                                                                                                  aTCAACCCCGTCTCCGCTGGAGATGCCGCCATTAATAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:790422/218‑1 (MQ=255)
                                                                                                                                                                                                                  aTCAACACCGTCCCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGGACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                           <  1:31642/178‑1 (MQ=255)
                                                                                                                                                                                                                      acccccGTTTCCTCTGAAGATGCCGCCATTATTAAAAATAACGGTCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaataat            <  1:986939/233‑1 (MQ=255)
                                                                                                                                                                                                                         cccGTCCCTTCTTTATATGCCGCCATTTTTAAAAATAACCATCGTCCCCGGCAGGTTATAACGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTc       <  2:469605/238‑1 (MQ=255)
                                                                                                                                                                                                                             tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACCACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACAACCACGGGGGTTATACATAACAAAAAAATTTCGGGc   >  2:871795/1‑239 (MQ=255)
                                                                                                                                                                                                                             tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACCCTAGCACCGATGGCATAGCCCATACCGATGCCCATAGACCCCCAGGCACCACAATCCAAACCACGACCAGGTTTATAccagcccctaaaacaaaacac   >  2:537718/1‑219 (MQ=255)
                                                                                                                                                                                                                             tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTACCACCTTCAATGGCGACAACCCGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGCCACCCCAGGAACCAAAATCCAGACAACACCGCGGGTTATAAAAATCAATAAAATATCGGGc   >  2:1084756/1‑239 (MQ=255)
                                                                                                                                                                                                                              ctcctcTGTAGATGCCGCCATTAATAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCa  <  1:1031380/239‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              
GGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCA  >  NC_000913/2490287‑2490746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: