BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,685,584	C→A	10.7%	intergenic (‑79/‑249)	glyA ← / → hmp	serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,584	0	C	A	10.7%	286.7 / 2.4	109	intergenic (‑79/‑249)	glyA/hmp	serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand): ref base C (35/60); new base A (12/2); total (47/62)
Fisher's exact test for biased strand distribution p-value = 8.54e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 110 total aligned reads displayed.

GGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAA  >  NC_000913/2685350‑2685822

ggTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTTGGCA‑ACGGCGAACAGTATAACCGaa                                                                                                                                                                                                                                                        >  1:206537/1‑239 (MQ=255)

    aGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑t                                                                                                                                                                                                                                                  <  1:547388/239‑1 (MQ=255)

      cTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTCTTGGACA‑ACACCGAACAGTATAACCGAA‑ACA‑Ttg                                                                                                                                                                                                                                                >  1:106337/1‑239 (MQ=255)

          gagcccTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTCCGATTTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAAGCGGCAATGTTCATTTCACGCTTTAACATCCGTATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGc                                                                                                                                                                                                                                             <  1:355351/235‑1 (MQ=255)

              cctggtccTGCATTTCGCGCGGGTTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATaa                                                                                                                                                                                                                                        <  1:450085/233‑1 (MQ=255)

              ccTGCCCCTGCATTTCCGGGGGGGTGGTGTAGTTTTCGGGGGCGATCAGTTCGATGTGCTCTTCCTGACTTTCCTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATaa                                                                                                                                                                                                                                        <  2:881687/239‑1 (MQ=255)

              aagggcccggccttccgcgcgGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACCTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATaa                                                                                                                                                                                                                                        <  1:161149/224‑1 (MQ=255)

                tGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACATTAAAATTTTTGCCCTTTAATGGCG‑GT‑CCCGGTGGAGA‑ACGGCGCCACGTAAAACCCAA‑CAA‑TTGTGCGGTAACa                                                                                                                                                                                                                                      >  1:233108/1‑239 (MQ=255)

                tGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGTCG‑GT‑CCTGTTTGGCA‑ACGGCGAAAAGATTAAACGAT‑ACA‑TTGTGCGCGAACa                                                                                                                                                                                                                                      >  1:511993/1‑239 (MQ=255)

                tGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTTGACA‑ACGGCGAAACGTATAAACGAA‑TCA‑TTGTGCGCTcaca                                                                                                                                                                                                                                      >  1:357046/1‑239 (MQ=255)

                tGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCCTTTGGGCA‑ACGGGGAACAATATAAACGAA‑TCA‑ATGTGCGGTaaac                                                                                                                                                                                                                                      >  1:208882/1‑237 (MQ=255)

                    ccTGCATTTCGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGt                                                                                                                                                                                                                                   <  1:1010394/238‑1 (MQ=255)

                            acgggggggCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACaaa                                                                                                                                                                                                                         <  1:944421/233‑1 (MQ=255)

                             cgcgcgGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGTCCTTTATAGGCG‑GT‑CCTGTTGGGCA‑ACGGCGAACCATATAACCGGA‑TCA‑TTGTGCTGTAACAGGG‑CTTGACAAA‑g                                                                                                                                                                                                                       >  1:949298/1‑239 (MQ=255)

                             cgcgcgGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGTCA‑ACGGCGCAACGGATCACCCAA‑TCC‑TTTTGGGATAACAGGG‑CTTGACCAA‑g                                                                                                                                                                                                                       >  1:1029864/1‑239 (MQ=255)

                                  gggCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAACTTTTTGACCTTTATAGGCG‑GT‑CCTGTTGGACA‑CCAGCGAACAGTATAACCCAA‑TCA‑TTGTTGGCTAAAAGGA‑CTTGa                                                                                                                                                                                                                             >  2:161149/1‑229 (MQ=255)

                                  gggCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCCGTTTGACC‑ACGGCGAAAAGGATAACCCGA‑TCA‑TTGTGGCGTTACAGGG‑CTTGGCAAA‑AGaaat                                                                                                                                                                                                                  >  1:990182/1‑237 (MQ=255)

                                   ggCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCCGTTGGGCA‑ACGGCGACAACAAATACCCAA‑TCA‑ATTTGCGATAAAAAGTGCTGGAAAAA‑GAAAtt                                                                                                                                                                                                                  >  2:214136/1‑239 (MQ=255)

                                          ttttttttCGGGGGCTATCGTTTCGATGGGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCaa‑a                                                                                                                                                                                                        <  2:357046/235‑1 (MQ=255)

                                              ttttCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTTGGCGCTAACAGGT‑CTTTGCAAA‑GGGATTTA‑CCCAA‑ACG‑At                                                                                                                                                                                                   >  1:198985/1‑239 (MQ=255)

                                              ttttCGGAGGCCATCAGTTCGATGTGCTTTTCCTGACGTTCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑At                                                                                                                                                                                                   <  1:987300/239‑1 (MQ=255)

                                               tttCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑At                                                                                                                                                                                                   <  1:465727/238‑1 (MQ=255)

                                                ttCGGAGGCGATCAGTTCTATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCCTCTGTACA‑ACCCCGAACAGCATAACCCAC‑TCA‑TATTGCGCTAACATGG‑CTGGCACAA‑GGAAATTC‑CCCAA‑ACG‑aatg                                                                                                                                                                                                 >  2:268638/1‑236 (MQ=255)

                                                  cGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑ACTGTTGGACA‑ACAGCGAACAGTATAACCGAA‑TCA‑ATTTGCGATAACAGGG‑CTTGACAAA‑GGAATTTA‑AGCAA‑ACG‑ATTa                                                                                                                                                                                                 >  1:213330/1‑237 (MQ=255)

                                                   ggAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTTGACA‑AACGCGAAAAGTATAACCCAA‑TCA‑ATGTGGGATAAAAAGT‑CTTGACAAA‑AGAAATTA‑AGCAA‑AAA‑ATTAACt                                                                                                                                                                                              >  2:678279/1‑239 (MQ=255)

                                                         gttCAGTTTGTTGTGCTCTTCTGGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAgg                                                                                                                                                                                         <  2:818998/236‑1 (MQ=255)

                                                         gctcctttctttttgCTCTTCCTCAAGTCCTTTTCCCTTCTCCATGGCCTGCCACAGTTCGGCATCATAATCGGCAATTTTCATTTCACTCTTTAACATCCGCACCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTCTAACCGAA‑TCA‑TTGTGCAATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTGCCTTCAgg                                                                                                                                                                                         <  2:939938/225‑1 (MQ=255)

                                                          aTCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GTCCCTTTGGGCCA‑ACGG‑GCACAATATAAACGCA‑TCA‑TTTTGGGGTAACAGGG‑CCTGAAAAA‑AGAAATTA‑ccaaaaacattaacctacagct                                                                                                                                                                                         >  2:180434/1‑218 (MQ=255)

                                                               tttgtttttgtctgtcTGACCTTCTTTTTCCTGCGCCATAGCCTTCCATCTTTCCGGATCATAATCGGCAATGTTTATTCCACGCTTTAACATCCGCAGCACCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑a                                                                                                                                                                                <  2:213330/224‑1 (MQ=255)

                                                                tcgtttgttctttccttccGTTCTTTTCCCTGCTCCAAACCCTGCCCCAGGTCGACATCATAATCGGCAATGTTCCTTTCCCGGTTTAACATCCGCATCTCCTGCCTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAGA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑a                                                                                                                                                                                <  2:303824/227‑1 (MQ=255)

                                                                tCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑a                                                                                                                                                                                <  1:1067920/238‑1 (MQ=255)

                                                                  gATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAAACGAA‑TCA‑TTTTGCGATAACAGGC‑CTTTGCAAA‑GGAAGTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACC‑C‑AAg                                                                                                                                                                              >  1:103588/1‑238 (MQ=255)

                                                                    tgtgCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCt                                                                                                                                                                           <  1:4756/239‑1 (MQ=255)

                                                                    tgtgCTCTTCCTGAAGTTTTTTTTCCTGCCCCATAGCCGGCCCCAGGTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCt                                                                                                                                                                           <  2:106337/239‑1 (MQ=255)

                                                                    tgtgCTCCTCCTTACGTACCTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAAGCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAAGTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTACAGGCTACG‑C‑AAGGCt                                                                                                                                                                           <  1:787469/239‑1 (MQ=255)

                                                                        gctttCCTGACTTTCTTTTTCTTGCCCCAGAGCCTGCCCAAGTTCCGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCCCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTgg                                                                                                                                                                      <  2:71249/236‑1 (MQ=255)

                                                                        ctctTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTgg                                                                                                                                                                      <  1:288719/239‑1 (MQ=255)

                                                                                gACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGTCG‑GT‑CCCGTTTGTCT‑ACGGCTGACAAGTTAAACGAA‑ACATTTGTCCTATAACAGGA‑CTTCAGAAC‑GCAATTAC‑CTCAA‑ACA‑CTAACTTTCA‑GCAACGAC‑AGGGCT‑TTGGAaaaa‑aa                                                                                                                                                              >  2:852714/1‑239 (MQ=255)

                                                                                     aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑Cg                                                                                                                                                                                                             <  2:441401/192‑1 (MQ=255)

                                                                                     aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑Cg                                                                                                                                                                                                             >  1:441401/1‑192 (MQ=255)

                                                                                     aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑At                                                                                                                                                                                                   <  1:1033294/200‑1 (MQ=255)

                                                                                     aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CACAA‑ACG‑At                                                                                                                                                                                                   >  2:1033294/1‑200 (MQ=255)

                                                                                     aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACAGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGG‑CTTGACAAA‑AGAATTTAGCGCAACACT‑A‑TA‑CTTCAGGGTAAC‑C‑AAGGGT‑TTTGAGAAA‑AAAAAGCt                                                                                                                                                        >  1:409066/1‑239 (MQ=255)

                                                                                     aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGCCG‑GT‑CCTGTTGGGCC‑ACGGCGAACAGTATAACCCAA‑TCA‑TTGTGCGATAACAGAG‑CTTGACCAA‑GGAATTTA‑AGCAA‑ACA‑ATTACCATCAAGGTACG‑CAAAGCCT‑TTGgaaagaaaaaaaa                                                                                                                                                          >  2:390779/1‑228 (MQ=255)

                                                                                                cTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACCGCGCACAGTTTAAACCAA‑TCA‑TTGTGCGGTAACAGGT‑CTTGGCAAA‑AGAATTTA‑CCCAA‑ACC‑CTTACCTTTCGGCCAAC‑C‑AAGGCT‑TTGGAGaaa‑aaaaaagctggaacagaaa                                                                                                                                             >  2:890322/1‑223 (MQ=255)

                                                                                                 tCCATACCCTGCCAAAGTTCCGCATCATAATCGGCAATTGTCAATTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGaaa                                                                                                                                           <  2:333067/239‑1 (MQ=255)

                                                                                                     tAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGGCA‑ACGGCGAAAAATATAACCGAA‑TCA‑TTTTGCGATAACAGGC‑CTTGGCAAA‑GGAATTTA‑CCCAA‑AAG‑ATTACCTTATGGCTACC‑A‑CAGCCT‑TTGGGGaaa‑aaaaaaactctaacccgaaaagaa                                                                                                                                        >  2:875028/1‑218 (MQ=255)

                                                                                                     tAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACg                                                                                                                                                                                    <  2:178339/199‑1 (MQ=255)

                                                                                                      gaccgtccACATGTCGGCATCATAATCGGCAATTTTCAGTGCACGCTTTACCTTCCGCATCGCCTGACTCAGCTAACAATAAATTTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGGGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGAt                                                                                                                                      <  1:359906/233‑1 (MQ=255)

                                                                                                         cTGCCAAAGGTCGGCATCATAACCGGCAAGGTTCATTTCCCGGTTTAACATCCGCATCTCCTGGCCCAGCTAACAATAAAA‑TTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCt                                                                                                                                  <  1:486488/239‑1 (MQ=255)

                                                                                                          tGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAACTTTTTGGCCTTTATAGGCG‑GT‑CCCGTTTGAGA‑ACTGCGCAAAAATTAACCGAATTCT‑TTGTGTGTTAA‑AGGG‑CTTGCCAAAGGGATTTTG‑CGC‑A‑ACG‑AAAACCATCATGGCACG‑C‑CAGGCT‑TTGGGGAATAAAAGAGATACC‑AACCGGaaaggagttta                                                                                                                                   >  2:982457/1‑231 (MQ=255)

                                                                                                          tGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAAACCAA‑TCA‑TTTTGCGATAACAGGG‑CTTGCGAAA‑GGAAATTA‑AGCAA‑ACG‑ATTACCTTCCGGCTACG‑C‑CAGGCC‑TTGGAGAAA‑AAAAAACGTGC‑AAACGGaaaacgagtttt                                                                                                                                  >  2:148729/1‑230 (MQ=255)

                                                                                                          tGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAAAAGAATAAACGAA‑TCA‑TTTGGCGGTAACCAGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATATCCTTCAAGCTAAG‑CAAAGGCT‑TT‑GAGAAT‑AAAAAGCTTGC‑CACCGGAAACGGAttttt                                                                                                                                  >  1:402865/1‑237 (MQ=255)

                                                                                                             cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCtttt                                                                                                                               <  1:180434/239‑1 (MQ=255)

                                                                                                                                    aaTGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTTGCCTTTATAGGCG‑GT‑CCTGTTTGACACACGG‑GAAAAGAATTAACCAA‑TCA‑ATTTGGACTAAAAGGG‑TTTGACAAA‑GGAATTTA‑AGCAA‑AAG‑ATTACCTTCAGGATACG‑C‑AAGGGT‑TTGGGGAAT‑AAAAACACTGCAAACGGGAAACGGTttttttttagggatgggaggaaaaatttt                                                                                                          >  2:36001/1‑217 (MQ=255)

                                                                                                                                    aaTGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTTGACA‑ACCGCGAACAGAATAACCGAA‑TCA‑TTGTGCGAATACAGGG‑CCTGACAAA‑GAGAATTA‑CGCCA‑ACA‑ATTACCTTCAGGCTCAG‑C‑AAAGCC‑TTTGGGAAT‑AAAAAACTTGC‑CACCCCAAAAGGGTTTTTTTTTTAGTTTGTTGTGAAAAatttta                                                                                                        >  2:729221/1‑238 (MQ=255)

                                                                                                                                         tcctttCACGCTTTAACATCCGCATCTCCTTACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACt                                                                                                     <  1:36486/234‑1 (MQ=255)

                                                                                                                                                  gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TAA‑TTGTGCGATAAAAGGT‑CTTGACAAA‑GGAAATTA‑CGCAA‑ACG‑ATTACCTTCAGGCCACG‑C‑AAGGAC‑TTTGAGAAT‑AAAGAGCTTGC‑AACCGGGAACGGATTTCTTTTCAGGTTTGTGAAGCAAAATTTTTACTtcatcaact                                                                                            >  1:394102/1‑234 (MQ=255)

                                                                                                                                                      tAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTTGACA‑ACGGCGAACAATATAACCCAA‑TAC‑TTTTGTCATAAACGGTACTTG‑CAAA‑AGAATTTA‑AGCAA‑ACG‑ATTAACTTTAGGCTAAG‑C‑CAGGCT‑TTTGGAAAT‑AAAAAAGTTTC‑AACCTGAAACAGCGTTCTTTTTAGGTTTTTGATGTAAATTTTTTACTTTATCCCAttctttt                                                                                      >  2:943553/1‑238 (MQ=255)

                                                                                                                                                       aaCATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TAA‑TTGTGCGATAACAGGG‑CTTTACAAA‑GGAATATA‑CGCAA‑ACG‑ATTACCTTCAGGGTACG‑C‑AAGGCT‑TTTGGGAAT‑AAAAAGCTTGC‑AACCGGAAAAGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTTCCTTCATACAAttctttct                                                                                     >  1:232764/1‑239 (MQ=255)

                                                                                                                                                           tccggagtctctagcTCAGCGAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑TCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGaaa                                                                                 <  1:280702/225‑1 (MQ=255)

                                                                                                                                                           tCCGGATTTCCTTACTAATCTAACAATAAAATTTTTGGCCTTGATAGGGG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGaaa                                                                                 <  2:103588/239‑1 (MQ=255)

                                                                                                                                                           tCCGCATCGCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGaaa                                                                                 <  1:583745/239‑1 (MQ=255)

                                                                                                                                                             cggaTCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGaaaaa                                                                               <  1:943553/236‑1 (MQ=255)

                                                                                                                                                             cGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGGCTTTATTGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGaaaaa                                                                               <  1:1025772/239‑1 (MQ=255)

                                                                                                                                                             cGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGaaaaa                                                                               <  1:890322/239‑1 (MQ=255)

                                                                                                                                                                  cccctttatCAGGTAACAATAAAATTTTTGGCCTTTATGGGCG‑GG‑TCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCa                                                                          <  2:198985/231‑1 (MQ=255)

                                                                                                                                                                    ccttgcTCAGTCAACAATAAAATTTTTGGCCTTTATAGGCG‑GG‑TCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATATA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCaaa                                                                        <  2:232764/234‑1 (MQ=255)

                                                                                                                                                                    ccTTACTCAGCTAACAATAAAATTTTTGGCCTTTAAAGGGG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCaaa                                                                        <  2:1050306/239‑1 (MQ=255)

                                                                                                                                                                    ccTGACTCAGCTAACAATAAAATTTTTGGCCTTTAGAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAAACGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCaaa                                                                        <  1:1068095/239‑1 (MQ=255)

                                                                                                                                                                    ccTGACCAAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCaaa                                                                        <  1:63605/239‑1 (MQ=255)

                                                                                                                                                                    acttgttagagacaaaaataaaTTTTTGGGCTTTAGAGGTG‑GT‑TCTAGTGGAAA‑AAGGGGTACAGTATAACCGGA‑TTA‑TTAGGAGAAAACAGGT‑CATGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGTTTGC‑AAACTGAAACGGAGTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCaaa                                                                        <  2:272485/220‑1 (MQ=255)

                                                                                                                                                                     cTGACTCAGCTAACAATAAAAGTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAg                                                                       <  1:268638/239‑1 (MQ=255)

                                                                                                                                                                      tgtcgcaagtAACAATAAAATTTTTGGCCTTTTAAGGGG‑GG‑CCTGGTGGACA‑ACGGCGAAAAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGG‑CTTGACAAA‑GGAATTTA‑CGGAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCCCTTAATCACATGCTTTCTGAAAAACACCAAAGa                                                                      <  2:268178/230‑1 (MQ=255)

                                                                                                                                                                          ctaaccaaCAATAAAATTTTGTGCCTTTTATGGCG‑GT‑TCTTTTGGACA‑ACGGACAACAATATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGAAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAAcc                                                                   <  2:511993/232‑1 (MQ=255)

                                                                                                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACCGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCAATTCATCACATTCTTTCTGAAAAACACCAAAGAACCAtt                                                                >  1:788503/1‑239 (MQ=255)

                                                                                                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTTACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGGGAAT‑AAAGAGCTTGC‑AACCGGGAACGGGTTTCTTTTCAGGTTTTTGATGCCAATTTTTTACTTTATCACATTCTTTTTGTAAACAAACAAAGAACCCtt                                                                >  1:716722/1‑239 (MQ=255)

                                                                                                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑a                                                                                                                                                                                <  2:718566/130‑1 (MQ=255)

                                                                                                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑a                                                                                                                                                                                >  1:718566/1‑130 (MQ=255)

                                                                                                                                                                            aGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACCGCGAAAATTAAAACCCAA‑TCA‑CTGGGCTATTACTGGT‑CTTGGCTAA‑AGAATTTA‑ACCAC‑ACGAATCTCCTCCAGCCTAC‑‑C‑ACGGCTGTTGGAGATA‑ATTCCAATTTC‑AACCGGAACCGGTTTCCTTTTTCGTTTTG‑GATGTACATTTTTCCCTTCCTCCCCTTTTTTCTTTAAAACACCCAACAACCact                                                                >  2:167477/1‑237 (MQ=255)

                                                                                                                                                                             gCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAAACGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑AGAATTTT‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGGGAAT‑AAAGAACTTGG‑AAACGGGAACGGATTTCTTTTCAGGTTTGTGTTCGAAAATTTTCACTTAATCACATTCTTTTTTAAAAAAACAAAAAAACAAttt                                                               >  2:324645/1‑239 (MQ=255)

                                                                                                                                                                                   aaaaaaaTTTTTGGCCTTTATAGGCG‑GT‑ACTGGTGGAAA‑AGGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATGTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCaaa                                                                        <  2:587068/221‑1 (MQ=255)

                                                                                                                                                                                   aaTAAAAGTTTTGGCCTTTTTTGGCG‑GT‑CCTTTTGGACA‑ACGGCGAACAGTAAAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CGTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCGGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTg                                                         <  2:208882/239‑1 (MQ=255)

                                                                                                                                                                                     aaaaaATTTTGGCCTTTTTAGGCGTGT‑CAT‑TTGGACA‑ACGGGGAACAGTTATACGCAA‑TCA‑GTGTGCAATAACAGGT‑CTTGGCAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCa                                                       <  2:688342/238‑1 (MQ=255)

                                                                                                                                                                                             ttGGCCTTTATAGGCG‑GT‑TCTGGTGGACA‑ACGGCGAACAGTATAACCCAA‑TCA‑TTTTGCGGTAACAGGT‑CTTGACCAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCCTCAGGCTACG‑C‑AAAGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTTAGGTTTCTGATGCACATTTTTCACTTCATCACATTCTTTTTGATAAAAACCACAGTAACATTTaacattttattataa                                                >  1:587068/1‑224 (MQ=255)

                                                                                                                                                                                             ttGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGG‑CTTGACAAA‑GGGATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACC‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCCGGTTTGTGATGCAAATTTTTTACTTCATCACCTTCTTTCTGAAAAACACCAAAGAACCATTTACATTTCAGGGCCAtt                                               >  1:45598/1‑239 (MQ=255)

                                                                                                                                                                                             ttGGCCTTTATAGGCG‑GT‑CCTGGTGGACA‑ACGGGGAACAGTATAAACGAA‑TAC‑TTGTGCGGTAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑AAG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGATTGC‑AACCGGCAACGGAATTCTTTTCCGGGTTTTGATGCCAATTTTTCACTTCATCACATTCTTTCTGAAAAAAACCAAAGAAACATTTTCATTTGAGGGCTAtt                                               >  1:167149/1‑239 (MQ=255)

                                                                                                                                                                                                   tttATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTta                                          <  1:390779/238‑1 (MQ=255)

                                                                                                                                                                                                      ataGGCG‑GT‑CCTGTTGGACA‑AAGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAg                                      <  1:683013/239‑1 (MQ=255)

                                                                                                                                                                                                        tgggg‑gT‑CCTGTTGGACA‑ACGGGGAACAGTAAAACCGAA‑TCA‑TTGGGGCATAACAGGT‑CTTTACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGAt                                    <  2:990182/235‑1 (MQ=255)

                                                                                                                                                                                                               t‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCAtt                               <  1:324645/238‑1 (MQ=255)

                                                                                                                                                                                                               gttcTGTTGGAAC‑ACGGCGAACATTATAAACGAA‑TCC‑TTGGGCGATAACAGGG‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCAtt                               <  2:324607/236‑1 (MQ=255)

                                                                                                                                                                                                                    tttGGAAA‑ACGGAGAACAGTATAACCGAA‑ACA‑TTGGGCGAAAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTgaga                          <  2:1029864/238‑1 (MQ=255)

                                                                                                                                                                                                                     ttGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGAt                         <  1:841265/239‑1 (MQ=255)

                                                                                                                                                                                                                             aCGGCGAACAGAATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTTTGAAAAAAACCAAAGAACCATTTACCTTGCAGGGCCATTTTTTATAAGATGCAtttagaaaacaccaa                  >  2:685165/1‑227 (MQ=255)

                                                                                                                                                                                                                              cGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGA‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAACGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAAt                 >  1:804053/1‑239 (MQ=255)

                                                                                                                                                                                                                                   aaCAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGa            >  1:876627/1‑239 (MQ=255)

                                                                                                                                                                                                                                      aGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTgaga                          <  1:823406/222‑1 (MQ=255)

                                                                                                                                                                                                                                         ataACCGAA‑TCT‑TTGTGCGATAACAGGT‑CTTGAAAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCaaa      <  2:716722/239‑1 (MQ=255)

                                                                                                                                                                                                                                             ccGAA‑TCA‑TTGTGCGATAACAGGA‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTACAGGGCTATTTTTTAAAAAATGCAATTGAGATACATAAATTAAAAAGCaaaaaaa  >  2:55416/1‑239 (MQ=255)

GGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCG‑GT‑CCTGTTGGACA‑ACGGCGAACAGTATAACCGAA‑TCA‑TTGTGCGATAACAGGT‑CTTGACAAA‑GGAATTTA‑CGCAA‑ACG‑ATTACCTTCAGGCTACG‑C‑AAGGCT‑TTGGAGAAT‑AAAGAGCTTGC‑AACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAA  >  NC_000913/2685350‑2685822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`