Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,718,257 A→C 14.6% S90R (AGT→AGG yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,718,2570AC14.6% 153.4 / 4.0 65S90R (AGT→AGGyfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (30/23);  new base C (5/7);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGTGCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTG  >  NC_000913/2718020‑2718491
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
ttCCTTGCCTTGCGCTTTTTTCAATTCCGCTTCATCCCCCACATGGTACGCTTTGCTGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCAc                                                                                                                                                                                                                                              <  2:933443/239‑1 (MQ=255)
     tGCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCCGGTTTCTTCCGCACGCTGACGACAGCAAACCTCCCGCTCACAAAAAACTTTACCAccact                                                                                                                                                                                                                                          >  2:967747/1‑234 (MQ=255)
      gCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATg                                                                                                                                                                                                                                         <  1:678619/238‑1 (MQ=255)
          gACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACCCTGACGCCCCCACACTTCCGGTCCAATAAAACATTTACCACGGAAGcgcac                                                                                                                                                                                                                                    >  2:684615/1‑235 (MQ=255)
               ttttGTCCATTCCCCTTCATCCACCACATGGTACGGTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACCCTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGa                                                                                                                                                                                                                                <  1:751242/238‑1 (MQ=255)
               ttttGTCAATTCCGCTTCATCCACCACATGGTACGGTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAACCGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGAt                                                                                                                                                                                                                               <  1:926463/239‑1 (MQ=255)
                       aTTCCGCTTCATCCACCACATGGTACGCTTTGCGGTCTGCTCCCATCCAGCGCCACGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCATGCACGGACAATCTCTTCCGGACGGCTCTGGCACAGTGCCTGACACGCCTATTCGCCGCAGACGAGGGTTTCTTCTGCACGCTGACGAAGCAACACTTCCGGGTCAATATAACTTTTTCACCCGAAGCCACCggttatggacttt                                                                                                                                                                                                                       >  2:1070462/1‑227 (MQ=255)
                                            ggTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACCCAACCCTTCCGGATCAATAAAACTTTTACCCCTGATGCCACCCGGGTCGGCCTTTTCCGGCgcaccactcaccgg                                                                                                                                                                                                   >  2:129133/1‑225 (MQ=255)
                                              tACGCTTTGCGGTTTGCTGCCATCCAGCGAAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGgcg                                                                                                                                                                                                <  1:927699/238‑1 (MQ=255)
                                                 gCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCCCGCTGACGCCGCAACACTTCCGGATCAATAAAACTTTTACAACTCATGCCACCGTGAACGGCCTTTTCCGGCGTCCCATCACCCGCcgcgcg                                                                                                                                                                                            >  1:1065765/1‑239 (MQ=255)
                                                 gCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGCCCTTTTCCGGCGTCTCATCACCCGGcgcgcg                                                                                                                                                                                            >  1:49771/1‑239 (MQ=255)
                                                    ttGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGCCATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGAACCCTGACGCCCAAACACTTCGGTATCAATAAAACTTTTACCCCCGTAGCCACCGTGACAGCCCTTTTCCGGCTCGTCACAACCCGACGCTCgggc                                                                                                                                                                                         >  2:648912/1‑238 (MQ=255)
                                                    ttGCGGTTTGCGGCCATCCAGCGCAAAGCTACTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGCCATGCATTTTAGCCGTAGACGCGGGGTTCTTCCGCACGCTGACGCCGCAACACGTCAGGGTCAATAAAACGTTTACCCCTGATGCCACCGTTACCCGCCTTTTGCGGCTACTCCTTACCCCGGGCACGGGa                                                                                                                                                                                        >  2:1066815/1‑239 (MQ=255)
                                                     tGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCgggga                                                                                                                                                                                        >  1:880769/1‑237 (MQ=255)
                                                             gctgcCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCCCGCTGACGCCGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCCCCTCACCCGGCCCGCGGGAAACCTTgg                                                                                                                                                                                >  2:760000/1‑237 (MQ=255)
                                                              ctgcCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTACGCCCGCTGACGCCGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGCTCCGCCTTTTCCGGCGCCCCATCACACCCCCCCCGGGAAACCGGgcg                                                                                                                                                                               >  2:702948/1‑238 (MQ=255)
                                                               tgcCATCCAGCCGAAAGGTTATTTAAAGCGCGGCGTTACACCCTGGATAAACCAGGCGCGAACAATCGCGTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATGTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTgcgc                                                                                                                                                                              <  1:120991/238‑1 (MQ=255)
                                                                                 ttcttTAAAACGCGGGGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTTCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACt                                                                                                                                                           <  1:702948/239‑1 (MQ=255)
                                                                                     ttAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                                                                                                                                       <  1:681705/239‑1 (MQ=255)
                                                                                         aaCGCGGCTTTACCCCTGGGATAACCCAGGCCGGAAACATCGCTTCCGGACGGCTTTGGAAAAGTCCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCCCGCTGACGACGCAACACTGCCGGATCAATAAACCTTTTACCACTGATGCCGCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCa                                                                                                                                                   <  2:880769/239‑1 (MQ=255)
                                                                                         aaCGCGGCGTTACCCTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCa                                                                                                                                                   <  1:614710/239‑1 (MQ=255)
                                                                                          aCGGGGCGTTCCCCTTTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCAc                                                                                                                                                  <  1:252680/239‑1 (MQ=255)
                                                                                          aCGCGGCGTTACCCTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCAc                                                                                                                                                  <  1:967747/239‑1 (MQ=255)
                                                                                              ggcgTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCCCGCTGACGACGCACCACTTCCGGATCAATAAAATTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGCCCCATTACCCGGCGCGCGGGAAACCGTCCCCCCCTGCGAACCCTCCCCCTTACggcccggggg                                                                                                                                              >  2:614710/1‑231 (MQ=255)
                                                                                                    acacTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                                                        <  1:133632/239‑1 (MQ=255)
                                                                                                        tctGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCCCTTGCGGTCACGGGGCTGACTCTGt                                                                                                                                    >  1:278677/1‑239 (MQ=255)
                                                                                                          tGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGCTGCCACCGGGATCGGCCTTTTCCGGCGCCTATTTACCCGTGCGGCGGGAAAACCGGGCCCACGGCGAACCTTCCCCCTTTCCGTCACcgggtgaacacctatg                                                                                                                                  >  2:435902/1‑227 (MQ=255)
                                                                                                          tGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                                                                                                                                  >  1:170619/1‑239 (MQ=255)
                                                                                                            gATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGGGATCGGCCTTTTCCGGCGGCCCATTAACCAGCGCGCGGGAAACCGTGCGCCCCGGCCAACTCTCCCACTTGCGGTCACGGGGcggcccccgcttc                                                                                                                                 >  2:150308/1‑226 (MQ=255)
                                                                                                                  ccAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCCCTTCCGGTCACGGGGCGGACTCTGTTTTTCATccc                                                                                                                          >  1:681834/1‑237 (MQ=255)
                                                                                                                      gcgcGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGCCCCTGTTTGTCATCccgggc                                                                                                                      >  1:241147/1‑239 (MQ=255)
                                                                                                                      gcgcGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCccggcc                                                                                                                      >  1:855577/1‑237 (MQ=255)
                                                                                                                      gcgcGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCCCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACGCTGTTTGTCATAccgggc                                                                                                                      >  1:246921/1‑239 (MQ=255)
                                                                                                                      gcgcGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGGCTCATCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCCCTTGCGGTCACGGGCGCGCCTCTGTTTgcactaccgggg                                                                                                                      >  2:720771/1‑228 (MQ=255)
                                                                                                                             ccttCGCTTCCGGACGGCTAGGGAAAATTGCCTTGCATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGGTGGCGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCTCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCGTCCCGCTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGa                                                                                                               <  2:1065765/236‑1 (MQ=255)
                                                                                                                                                        gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTAATCGCGGGCGGGGCGCCGGCCACCGCCCGCACgagggttt                                                                                       >  1:305856/1‑231 (MQ=255)
                                                                                                                                                        gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCCGCTTGTCATCGCGGGCCGGGCGACGGACACCGCAAGACCGACAtttt                                                                                       >  1:985224/1‑236 (MQ=255)
                                                                                                                                                        gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTCCGCCACGGCGGATCTTCCCCCTTGCGGTCACGGGGCTGACTCTGTTTTGCATCGCGGGGGGGGCGCGGGCCCACCCCAGACCGAGATTTTccg                                                                                    >  2:651423/1‑238 (MQ=255)
                                                                                                                                                        gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGCCATCGCGGGCGGGGTGACGGCCCCCGCCACGACGGAAtttt                                                                                       >  1:820040/1‑236 (MQ=255)
                                                                                                                                                           ccTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTTCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGCCAGCACGACATTTTCCTgg                                                                                 >  1:206028/1‑238 (MQ=255)
                                                                                                                                                                cATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGGCGCAACACTTCCGGATCAATAAAACTTTTACCACGGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                                                           <  1:435902/239‑1 (MQ=255)
                                                                                                                                                                           gCCGTAGCCGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTcc                                                                <  1:720771/239‑1 (MQ=255)
                                                                                                                                                                              gTAGACGCGGGTTTCTTCCGCACGGCGACGACGCAACCCTTCCGGATCAATAAAACTTTTACCCCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCgg                                                             <  1:108813/239‑1 (MQ=255)
                                                                                                                                                                               tAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGGCTCTGTTTGTCATCCGGGGCGGGGGGACGGCCACCGCCACCCAGGACTTTTCCTGGGAGGCCAGCCCCCTTCCCGGt                                                            >  2:392546/1‑239 (MQ=255)
                                                                                                                                                                                            cttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                               <  1:651423/239‑1 (MQ=255)
                                                                                                                                                                                                cGCACGCTGCCGACGCAACACTTCCGGATCAATAAAACTTTTACCCCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCCTCGCGGGCGTGGCGACGGCCACCGTCCGGACGAGATTTTCCTGGGCGCCCCCCCCCTTTCCCCGGCCCCGGGTCGTggttga                                           >  2:1062516/1‑235 (MQ=255)
                                                                                                                                                                                                 gcccGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCCCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACg                                          <  1:129133/236‑1 (MQ=255)
                                                                                                                                                                                                        ggcgccgCAACCCCTCCGGATCAAAAAAACTTTTCCCCCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTTCGGCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                                   <  2:855577/234‑1 (MQ=255)
                                                                                                                                                                                                           cgccccaccccTTCCGGATCAATAAACCTTTTACCCCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                 <  1:684615/229‑1 (MQ=255)
                                                                                                                                                                                                           cgacccaccctttCCGGTTCAATAAAACTTTTCCCCCTGATGCCACCGTTATCGGCCTTTGCCGGCGTCTCATCCCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAACCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                 <  2:241147/228‑1 (MQ=255)
                                                                                                                                                                                                           ccacagacatcctCCGGAATAATAAACCTTTTCCCCCGGATGCCCGCCTGTTCGGCCTTTTCCGGGGGTCCCTCACCCGGCGCGCGGGAAACCGTGCGCCACGGCCAAACTGCCCTCTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTTGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                 <  2:753763/227‑1 (MQ=255)
                                                                                                                                                                                                                            ttCAATAAAACTTTTCCCCCTGATGCCCCCGTGATCGGCCTTTCCCGGCGGCTCATCCCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                <  2:883390/238‑1 (MQ=255)
                                                                                                                                                                                                                            aTCAATAAAACGTTTCCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCGCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                 <  1:904902/238‑1 (MQ=255)
                                                                                                                                                                                                                            aTCAAATAAACTTTTACCACGGATGCCCCCGTGAGCGGCCTGTTCCGGCGTCTCATCCCCCGGCGCGGGGGAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGGTTGGCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                 <  1:1062516/238‑1 (MQ=255)
                                                                                                                                                                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg                                                                          <  1:880940/177‑1 (MQ=255)
                                                                                                                                                                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg                                                                          >  2:880940/1‑177 (MQ=255)
                                                                                                                                                                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacata            >  1:883390/1‑239 (MQ=255)
                                                                                                                                                                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGCTGTGGGTACGTTTATCAGAATCATCATCCCTGCGGacaa             >  2:598726/1‑237 (MQ=255)
                                                                                                                                                                                                                                 tAAAACTTTCACCCCTGGTGCCACCGTGTTCGGCCTTGTCCGGCGTCCCATCGCCCGGCGCGCGGGAAACCGTTGGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTTACTCTGTTTGCCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacatac           <  2:246921/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaaCTTTTCCCAACGACGCCCCCTTGTCTGGCCTTTTCCGGCGTCCCCCCCCCCGGCACGCGGGAATCCGTCCCCCACGGCGAATCTTCCCACTTGCGGCCACGGGGCTGAATCTGTTTTGCAGCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacataca          <  2:985224/239‑1 (MQ=255)
                                                                                                                                                                                                                                       ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                               <  1:563067/197‑1 (MQ=255)
                                                                                                                                                                                                                                         ttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGATTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGTTTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGGTTATCATAATCATCATCACTCCGGACATACATCACttt   >  1:722382/1‑239 (MQ=255)
                                                                                                                                                                                                                                         ttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACCCGGGTTGTGGGTACGTTTATCCGAATCATCATCCCTCCGGACATACATCCCtt    >  1:176661/1‑238 (MQ=255)
                                                                                                                                                                                                                                           aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCCCTTGCGGTCCCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGCCGGCCCCCGTCAGCACGCGATTTTCTTGTCCGCCCCCCCCCTTTCCCGGTACCCGGGTTTTGGTTCCGTTTTTCAGAACCCTCATCCCAGCGGCCCACCATCCCTTTg  >  2:623193/1‑238 (MQ=255)
                                                                                                                                                                                                                                           aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGCTCGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGGTGTGGGGACTTTTTTCAGAATCACCATCCCTGCGGCCAAAAATCACTTTg  >  2:618114/1‑238 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
TTCCGTGCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTG  >  NC_000913/2718020‑2718491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: