BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,027,729	A→G	13.1%	E204G (GAG→GGG)	ghxQ →	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,027,729	0	A	G	13.1%	295.7 / 4.5	123	E204G (GAG→GGG)	ghxQ	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand): ref base A (31/71); new base G (15/4); total (48/75)
Fisher's exact test for biased strand distribution p-value = 1.42e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

ATCTTCACCGCCATTTCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTGG  >  NC_000913/3027494‑3027957

ttCTTCCCCTCCTTTTCCGTAACGGGTGGTCGTCCCGGGGTCTTACTAAATTTGCCGATGGGTATCGGTCACGGTACGGGTATCGGTATCGGGCTGTTTCTGG‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑GGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGaa                                                                                                                                                                                                                                        <  2:792151/238‑1 (MQ=255)

     cACCGCCATTTCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGCGATGATGAGCTTGCTGGGGCTGGC‑GGCCATCTTCGGCCTTGGGAA‑gggt                                                                                                                                                                                                                                   >  1:477398/1‑238 (MQ=255)

           atagggacaggcctgggtgCTTACCTGGGTTTTACTTAATTTGGCGAGTGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTAc                                                                                                                                                                                                                           <  2:243212/223‑1 (MQ=255)

               ttcggtcccGGTGTGCGTTCCTGGGTTTTTCGGATGTTGCCGGTGGGTATCGCCCACGGTAGAGGGATTGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCcgg                                                                                                                                                                                                                       <  2:684990/232‑1 (MQ=255)

               tCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCcgg                                                                                                                                                                                                                       <  1:651891/239‑1 (MQ=255)

                tgtgtattagacgtcGTTCCTGGTTCTTAGTTAATTTGCCGAGGGGTATCTGTCCCGGTACAGGTATCGGTATCGTGCTGTTTCTGC‑TGCGGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCcgg                                                                                                                                                                                                                       <  2:1026448/224‑1 (MQ=255)

                         gtgtGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGGCTGGGGAA‑GGGTC‑GGGTGACCgggggggggttg                                                                                                                                                                                                             >  2:145846/1‑233 (MQ=255)

                          tgtgCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GGGTC‑GCGGACCCGGCGGG‑ACCtttt                                                                                                                                                                                                           >  1:521312/1‑237 (MQ=255)

                          tgtgCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGACTCTTCGGGCTGGAGAA‑GTGTG‑CCGTACCCGGCGGG‑CTCttgt                                                                                                                                                                                                           >  1:489275/1‑239 (MQ=255)

                            tgCGTACCTGGATCTTACGTAAGTTGCCGGTGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCttgttg                                                                                                                                                                                                         <  1:426865/239‑1 (MQ=255)

                               gTACCTGGTTCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGt                                                                                                                                                                                                       <  1:79497/238‑1 (MQ=255)

                                tACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGGC‑CCGTAACCGGCGGG‑agcctgcccctat                                                                                                                                                                                                     >  1:243212/1‑227 (MQ=255)

                                 aCCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGCGCTTGCTGGGGCTGGC‑GGTCATCCTCGGCCCTGGGAA‑AGGTC‑GCGGACCCCGCGGG‑GTCttttttggggt                                                                                                                                                                                                    >  1:183617/1‑233 (MQ=255)

                                      gATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GGGTC‑GCGTACCCGGCGGG‑AGCTTGTTGGGGgttatt                                                                                                                                                                                                >  1:873422/1‑238 (MQ=255)

                                           tACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGCATATTCCGCCCGGGGAA‑‑TGTCGGCTTACCCGGCGGG‑ATGTTTTTGGGGATGATTGaaaaa                                                                                                                                                                                          >  2:805148/1‑237 (MQ=255)

                                           tACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGCACCTTCGGCCTGCGGAA‑GGGTC‑CCGTACCCGGCGGG‑ACCTTGTTGGGTGTTATTTCAAtt                                                                                                                                                                                          >  1:109108/1‑239 (MQ=255)

                                           tACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGGGATGATGAGCTTGCTGGGGCTGGC‑GGGCATCTTCGGTCTGGGGAA‑GGGAC‑GCGTAACCGGCGGG‑ATCttgtctgtctcttttaccaca                                                                                                                                                                                          >  1:1026448/1‑222 (MQ=255)

                                                  tttGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑TTGTC‑GCGTACCCGACTGG‑AGCTTTTTGGGGATTATTTCAATTTT‑Gatca                                                                                                                                                                                  >  1:955434/1‑239 (MQ=255)

                                                  tttGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GGGTC‑GCGGACCCGGCGGG‑GTCTTGTTGGGGGTTATTGCAATTTC‑Gatca                                                                                                                                                                                  >  1:997212/1‑239 (MQ=255)

                                                        tgttgttttttctccgtgttccggtttcggtttcGGGGTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCt                                                                                                                                                                            <  2:326150/217‑1 (MQ=255)

                                                        gttGGGTTTTGCTCACGGTACGGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCt                                                                                                                                                                            <  2:835332/237‑1 (MQ=255)

                                                          ggtttgtccctgtcGGTACAGTGATGGGTTGCGGGGTGTTTCTGA‑TGCGAAGTGTTGGTAAG‑GGTGGGTGTA‑TGGTTATCAGAAACCCGATTGAACGATGGCCCGTGGT‑GCTCG‑GTGCGTTTAACTCCTTCCCGGTTAGGATTAGCTTGATGGGGCTGGT‑GGGCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTCCCCGGCGCG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCtgc                                                                                                                                                                          <  2:911766/226‑3 (MQ=255)

                                                               aTCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCtt                                                                                                                                                                     <  1:125406/239‑1 (MQ=255)

                                                                   cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCCTCTTCGGCCTGGAGAA‑GTGTC‑GCGGACCCGGCGGG‑ATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGa                                                                                                                                                                  >  1:528558/1‑238 (MQ=255)

                                                                    ctacgggtcAGGTTGCGGTATTGGGGTTGTTCTGCGTGTTG‑TTGCTGCGAAC‑GGTGTGGGTA‑GGGTTAGCAAAAACCCGGTTGGAGGCTTGGCAGTGGG‑GCTCG‑GTGCGGTTACCTCCTTCCCGGTGATGAGGAGCTTGCTGGGGGTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGAcc                                                                                                                                                                <  2:1067640/231‑1 (MQ=255)

                                                                        ggTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGCGAA‑GTTGC‑GCGTCCCCGGCGGG‑GTCTTGTTGGTGGTTTTTGCAATTTA‑GCTCATTGGCTTAAACTTTGACCCA‑Gc                                                                                                                                                           >  2:273836/1‑239 (MQ=255)

                                                                        ggTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCACCCTCGGCCTGGCGAA‑GGGTC‑GCGGACCCGGCGGG‑GGCTTGTTGGGGTTTTTTGCAATTTC‑CTTCATCGGGTTAATCCTTGAaccccc                                                                                                                                                            >  2:1377/1‑236 (MQ=255)

                                                                         ggcagggtatcggtaacGGGCTGTTTCGGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAGCCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑g                                                                                                                                                            <  1:313442/232‑1 (MQ=255)

                                                                          gaCAGGGATCGGTATCGGGCTGTTTCTGC‑TGCTTATGTCTGCTAAC‑GGTGTGGGTA‑TGGTTAACAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑g                                                                                                                                                            <  1:755550/235‑1 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGTCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑CCGGGGAAATa                                                                                                                                                  >  1:102399/1‑238 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAAGGTGT‑‑GCGGACCCGGCGGG‑GTCTTGTTGGTGATTATTGCAAATTC‑GGTCATCGGCGTAATCTTTGACCCA‑GCGGGGaaaaa                                                                                                                                                  >  2:387102/1‑236 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATAc                                                                                                                                                 >  1:497798/1‑239 (MQ=255)

                                                                                  tcggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGCCTTAATCTTTGACCCC‑GCGGTGAAATa                                                                                                                                                  >  1:227631/1‑238 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAG‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTggg                                                                                                                                                                                                       >  1:834241/1‑184 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATAcc                                                                                                                                                >  1:318946/1‑238 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCa                                                                                                                                               <  1:41765/239‑1 (MQ=255)

                                                                                    ggtatcggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGGGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTTCCCCA‑GCGGTGAAATAcc                                                                                                                                                >  1:639590/1‑238 (MQ=255)

                                                                                         cggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATTAGCTTGCTTGGGCTGGC‑GGTTACCTTCCGCCTGGGGAC‑GGGTC‑GCGTACCCCGCGGG‑CTCTTGTTGGTGATTATTGGAAGTTCGGATC‑TCGGTCTTATCTTTTACCCA‑AGGGGGAAATaaaccggtc                                                                                                                                          >  2:784210/1‑231 (MQ=255)

                                                                                         cggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GTGTC‑GCGTACCCGGCGGG‑GTGTTTTTGGTGGTTTTTGCAATTTG‑CATCATCGGCTTAATCTTTTACCCA‑GCGGGGaaaaaacatccat                                                                                                                                          >  2:313442/1‑229 (MQ=255)

                                                                                         cggGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCAACTTCGGCCTGGGGAA‑GTGTC‑GCGTAACCGGGGGG‑ATCCTGTTGGGGGTTATTGGAATTTC‑GATACTGGGGTTAATTTTTGACCCA‑CCGGGGGAAAACCCGGGTc                                                                                                                                          >  2:983608/1‑239 (MQ=255)

                                                                                            gCTGTTTCTGG‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCtg                                                                                                                                        <  1:638224/238‑1 (MQ=255)

                                                                                            gCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑Gatca                                                                                                                                                                                  <  1:501756/197‑1 (MQ=255)

                                                                                               gTTTCTGG‑TGGGGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCtggtgg                                                                                                                                    <  1:334674/239‑1 (MQ=255)

                                                                                                ttttTGT‑TGGTGTTTGTTGCTAAG‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGGAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGc                                                                                                                                   <  2:80064/239‑1 (MQ=255)

                                                                                                tttCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑GTCTTGTTGGTGATTATTGCAATTTC‑GGTCAACGGCTTTAACTTTGTCCCC‑CCGGGTGAATACCCCGGTCcgggggg                                                                                                                                   >  2:871030/1‑238 (MQ=255)

                                                                                                               tttctttatc‑GGTGGGGTTG‑TGGTTATTAAAAACCCGGTGTAGGGCTGGGCAGTGGG‑GGTCT‑GGGCGTTTTCCTCCTTCCCGGGGATGCTGAGCTTGCTGGGGCTGGC‑GGTCAGCGTGGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGa                                                                                                                     <  2:334164/230‑1 (MQ=255)

                                                                                                               tggttttAAC‑GGT‑TGGGTGTTGGTTATCAAAAACCCGATTGAAGGCTTGGCAGTGGG‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGa                                                                                                                     <  2:277118/233‑1 (MQ=255)

                                                                                                                 gctctAACGGGTGT‑GGTT‑TGGTTTTCAAAAACCCGATTGGAGGGTTGGCAGTTGG‑GCTCG‑GGGCGGTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACt                                                                                                                   <  2:1046303/236‑1 (MQ=255)

                                                                                                                               gggTT‑TGGTTATCAAAACCCCGATTGAAGGCTTGCCAGTTGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTa                                                                                                      <  1:848347/239‑1 (MQ=255)

                                                                                                                               gggTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGGTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGt                                                                                                       <  1:273836/238‑1 (MQ=255)

                                                                                                                               gggTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGGGCTTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑TCGGTGACATACCACGACCTGGTGGCGATGCCAAGCCCGACTGGCGAAAATGGtc                                                                                                      >  1:720208/1‑238 (MQ=255)

                                                                                                                               gggTA‑TGGTTAACAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCGTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGt                                                                                                       <  1:1377/238‑1 (MQ=255)

                                                                                                                                     tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGCAATACCACGGTCTGGTGGCGATGGCAAGCCTGACTGGCGAAGATGGGAAggc                                                                                                  >  1:1037963/1‑236 (MQ=255)

                                                                                                                                        ttAGCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGt                                                                                                       <  1:869609/230‑1 (MQ=255)

                                                                                                                                           tCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTTGGCCCGGAGAG‑GTGTC‑GCGTACCCGGCGGGCACCTTTTTGG‑GGTTTTTGCAATTTC‑GACCCTTGGCTTAATTTTTGACCCA‑GCGGTGGAATACCAACGTCTGGGTGCGATGCCAAGCCTGACCGGCGaaaaaggaaaacctcttctt                                                                                           >  2:245076/1‑221 (MQ=255)

                                                                                                                                           tCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑GTCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGGAAGCCTCTGAtt                                                                                           >  1:203556/1‑239 (MQ=255)

                                                                                                                                           tCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGGCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑AGCTTGTTGGTGGTTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCAGGCGGTGAAA‑ACCACGGGTCGGGAGCGGTGGCAAGGCCGACTGGCGGAGAAGGTaaaagcctggtt                                                                                           >  2:842421/1‑229 (MQ=255)

                                                                                                                                            cAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGCCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAttt                                                                                          >  1:130444/1‑239 (MQ=255)

                                                                                                                                                   ccGTTTTAAGGCTTTCCTGTGGC‑TCTCG‑GTTCGTTTACCTTCTTCCCGGTTATGATGAGGTTGCTGGGGCTGGC‑GGTCATCTGCGGCCTGGAGAA‑GTTTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCt                                                                                   <  2:541141/239‑1 (MQ=255)

                                                                                                                                                   ccGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTTCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑TTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCt                                                                                   <  1:765128/239‑1 (MQ=255)

                                                                                                                                                   ccGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCt                                                                                   <  2:497798/239‑1 (MQ=255)

                                                                                                                                                    cgttttaaGGCGTGCCAGTGGC‑GGCCG‑GGGCGTTTACCTCCTTCCAGGTGATGATGAGCTTGCTGGGGCTGGC‑GTTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:639590/233‑1 (MQ=255)

                                                                                                                                                    cggttGGAGTCTTGCCGGGTGC‑GTTCG‑GGGCGTTTCCCCCCGTCCCGGTGATGAGTAGCTTGCTGGGGCTGGG‑GGTTAATTTCGGCCGTGAGAA‑GTGTC‑GCGTTCCCGGCGGG‑ATCTTGGTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:477398/236‑1 (MQ=255)

                                                                                                                                                    cggttGAGGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTTCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  1:777803/236‑1 (MQ=255)

                                                                                                                                                    cggttGAAGGCTTGCCGGTGGC‑GGTGG‑GTGCGTTTACCTCCTCCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:444284/236‑1 (MQ=255)

                                                                                                                                                    cGATTGAATGTGTGCCAGGGGG‑GGTCT‑TGGCGTTTTCCTCTTTCCCGGTGATTATGGTGTTGCGGGGGCTGGC‑GGTCATCTTCGGCGTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTAATGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  2:183617/239‑1 (MQ=255)

                                                                                                                                                    cGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  1:983608/239‑1 (MQ=255)

                                                                                                                                                    cGATTGAAGCCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                                                  <  1:884721/239‑1 (MQ=255)

                                                                                                                                                      aTTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTTGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGCAGATGGGAAGTCTCTGATTTTCACACTCGa                                                                                >  1:223353/1‑239 (MQ=255)

                                                                                                                                                       ttGAAGGTTTGGCCGTTGC‑GCTCG‑GTGGGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGat                                                                               <  2:796695/239‑1 (MQ=255)

                                                                                                                                                           aGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtatta                                                                           <  1:598270/239‑1 (MQ=255)

                                                                                                                                                             gCTTGCCAGGGGC‑G‑TCGTGTGCGTTTTCCTCCTTCCCGGTGATTATGAGCTTGCGGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtattat                                                                          <  2:318946/238‑1 (MQ=255)

                                                                                                                                                              cTTGCCAGTGGCGGCTGG‑GT‑CGTTTCCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtattat                                                                          <  1:239771/237‑1 (MQ=255)

                                                                                                                                                                 gCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGGTTATTGCAATTTC‑GATCATCGGCTTAATCTTTTACCCA‑GCGGGGAAATACCACGGGCTGGGGGCGATTCCCACGCTGACTGGCGCAGAGGGGAAGGTCCTGATTTTTACCCCCGATATTAAggggg                                                                     >  2:295415/1‑237 (MQ=255)

                                                                                                                                                                 gCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCCCGGTCTGGTGGCGATGCCAAGCCTGACTGGCg                                                                                                               >  1:935334/1‑197 (MQ=255)

                                                                                                                                                                 gCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCg                                                                                                               <  2:935334/197‑1 (MQ=255)

                                                                                                                                                                      gtgcggcTCG‑GTGCGTTTACCTCCTTCCCGGTGATGGGGAGGTTGGTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACt                                                                 <  2:873422/234‑1 (MQ=255)

                                                                                                                                                                          cgttCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCa                                                              <  1:387102/235‑1 (MQ=255)

                                                                                                                                                                            ttCG‑GTGGTTTTTCTTCTTTCCCGGTGATGTTGGGGTTGCTGGGGGTGGC‑GGTCATCTTCGGCCTGGAGAA‑TTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGcc                                                           <  2:130444/238‑1 (MQ=255)

                                                                                                                                                                            cTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑GTCTTGTTGGTGATTATTGCAATTTC‑GGTCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAATTTCAGCCTCGATATTATGGGGGCAACCCAGcc                                                           >  1:42403/1‑239 (MQ=255)

                                                                                                                                                                            cTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGc                                                                                                                >  1:288514/1‑186 (MQ=255)

                                                                                                                                                                            cTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGc                                                                                                                <  2:288514/186‑1 (MQ=255)

                                                                                                                                                                             tCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGcc                                                           <  1:672521/238‑1 (MQ=255)

                                                                                                                                                                               tggggcttttACCTCCTTCCCGGTGATTGTGGGTTTGCTGGGGCTGGCGGGTCA‑CTTGGGCCTGGAGCA‑GTGGC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCAGCGTCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCaa                                                         <  2:109108/232‑1 (MQ=255)

                                                                                                                                                                                   gcctttCCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTTATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTg                                                      <  1:23464/235‑1 (MQ=255)

                                                                                                                                                                                         tccccctCCCCGGTGATGTGGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCGTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTcc                                               <  2:1054970/235‑1 (MQ=255)

                                                                                                                                                                                           cttcgttcgggtggtgttggcgttGCTGGGGCTGTG‑TGTTTTCTTCGGCCTGGAGAA‑GTGTC‑CGGTCCCCTGCGGG‑ATCTTGTTGGTGATTATTTCAATTTC‑GATCGTCGGGTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                                             <  2:42403/217‑1 (MQ=255)

                                                                                                                                                                                           ctcctTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                                             <  1:442061/239‑1 (MQ=255)

                                                                                                                                                                                               ttCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAgtgt                                         <  1:1038120/239‑1 (MQ=255)

                                                                                                                                                                                                tCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTTTGGGTGCACTCCAGCCAACTGTACTTCCCGgtgt                                         >  1:2453/1‑238 (MQ=255)

                                                                                                                                                                                                  ccGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTCCACTCCAGCCAACTGTACTTCCGAGTGTACt                                      <  1:145846/239‑1 (MQ=255)

                                                                                                                                                                                                       tgttgtgggtgttggggggCGGGC‑GGGCTTCTTCGGCCGGGGGAA‑GGGTG‑GCGTACCCGGCGGG‑ATCTTGTGGGTGATTAGTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCa                                  <  2:227631/223‑1 (MQ=255)

                                                                                                                                                                                                       tatggtggGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGGGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCa                                  <  1:919090/237‑1 (MQ=255)

                                                                                                                                                                                                       ataagggactgtGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAt                                 <  1:570777/228‑1 (MQ=255)

                                                                                                                                                                                                           gttggtttgggggggGTGGC‑GGTTATTGTCGGCCTGGAGAA‑GGTTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATGCCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGt                             <  2:2453/228‑1 (MQ=255)

                                                                                                                                                                                                           gtgaGCTTGTCGGTGCTGGC‑TGTCATCTTCGGCCTGGGCAA‑GTGTC‑GCGTACCGGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GGTCATCGGCTTAATCTTTGCCCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGt                             <  2:919201/238‑1 (MQ=255)

                                                                                                                                                                                                                cTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAATCCGGCCCGGCGCAGGTGGGAAGTCTCTGCTTTTTACCCTCGATATTATAGGTGCACCCCCGCCCACTGTACTTCCGCGGGTACAgggcatgggggta                         >  2:156127/1‑227 (MQ=255)

                                                                                                                                                                                                                cTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATAACACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTGTCTGATTTTTATCCCCGATATTTTGGGGGCACTCCAGCCAAATGTACTTCCCAGGGTACGGGCAATggggggga                        >  2:603670/1‑233 (MQ=255)

                                                                                                                                                                                                                                ggTCATCGTGGGCATGGAGAA‑GTGTC‑GCGTACCCGGTGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGAc          <  1:805148/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cttttttggCCTGGGGAG‑GTGTG‑GCTTTCCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTa      <  2:1020548/232‑1 (MQ=255)

ATCTTCACCGCCATTTCCGTAACCGGTGTGCGTACCTGGATCTTACGTAATTTGCCGATGGGTATCGCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGC‑TGCTGATTGCTGCTAAC‑GGTGTGGGTA‑TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC‑GCTCG‑GTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC‑GGTCATCTTCGGCCTGGAGAA‑GTGTC‑GCGTACCCGGCGGG‑ATCTTGTTGGTGATTATTGCAATTTC‑GATCATCGGCTTAATCTTTGACCCA‑GCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGGTGATGACCGCAGTGTTCGACGCTACTGG  >  NC_000913/3027494‑3027957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`