Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 706,050 Δ1 bp 5.7% intergenic (+160/‑43) nagE → / → glnS N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913706,0500A. 5.7% 224.9 / 4.8 70intergenic (+160/‑43)nagE/glnSN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (31/35);  new base . (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGCCAGTGCAACC  >  NC_000913/705819‑706282
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        
ggTGGCTTGAGCATTAAAGCTCAGGGCCATTTGTGGGCGGGCCAAACACCGTTGGATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                                                                                                                                                                                                                   <  1:574691/238‑1 (MQ=255)
   ggttttttctttAAAGCTCAGGGCCATTTTTGGGCGGGTCAAACCCCGCTTTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                                                                                                                                                                                                                   <  2:70023/226‑1 (MQ=255)
          attttAAAGCCCAGGGGCATTTTGGTGGGGGTCAAACACCTCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCGGATGCGACGCTTGGGCTTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACg                                                                                                                                                                                                                         <  2:314004/236‑1 (MQ=255)
                aagtccAGGGCCATTTTGGTGCGGGTCAAACCCCGGTTTATGAAATCAAAAAGGAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGttt                                                                                                                                                                                                                   <  2:1304804/234‑1 (MQ=255)
                aaGCTCAGGGCCATATTGTGGCGGGTCAAACTCCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGttt                                                                                                                                                                                                                   <  1:45979/239‑1 (MQ=255)
                           cATTTTGTGGGGGGTCAAACACCGGTGTATGAAAACAAAAAGTAATCTGTTTTATGCCTGATGCGACGCTTTGGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAg                                                                                                                                                                                                         <  1:600154/238‑1 (MQ=255)
                             tatTGGGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACg                                                                                                                                                                                                                         <  1:818855/220‑1 (MQ=255)
                                           cAAACACCGGTGTATGAAATCAAAAAGTAATCGGGTTTAAGCCTGATGCGACGCTTGAGCGTCGCATCCAACAAGGACAAGCGGTTGAGATTTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagt                                                                                                                                                                                          <  1:981531/238‑1 (MQ=255)
                                                    cTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTTAGGAATCCACGATGAGGAAGgaagaa                                                                                                                                                                                 >  1:676579/1‑238 (MQ=255)
                                                    cTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAt                                                                                                                                                                                              <  1:1157695/225‑1 (MQ=255)
                                                              tCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTTTAAGATCTTACGCCGTTATACGTTGTTTACCCTTTGAGGAATCCACAAAGAAGGAGGCaacgttctcttataa                                                                                                                                                                      >  2:1157695/1‑225 (MQ=255)
                                                              tCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCTTACGCCGTTATACGTTGTTTACGCTTTGGGGAATCCACGATGAATGAGGCAGAAGCCCGCCCGAc                                                                                                                                                                      >  1:1377873/1‑239 (MQ=255)
                                                                 aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGTCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAgcccg                                                                                                                                                                            <  2:914188/229‑1 (MQ=255)
                                                                 aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGTCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAgcccg                                                                                                                                                                            >  1:914188/1‑229 (MQ=255)
                                                                 aaaaGTAATCTACTTTATGCCTGATGCCACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGTTCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTTTCAGCCTGTCCCGCTTTTAGGATCATACGCCGTTATAGGTAGTTTACGCTTTGAGGAATCCACGTTAAGTCAGGACGAAGCCCGCCCGAcaa                                                                                                                                                                    >  1:610874/1‑236 (MQ=255)
                                                                                   gCCTGAGGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGatcat                                                                                                                                                 <  1:986550/239‑1 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGCTTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATCCGTTGTTTACCCTTTGAGGAATCCACGATGCGTGGGGCAGAAGCCCGCCCGGCTACCTTTATCCTTCAAatcatcaag                                                                                                                                             >  2:1286607/1‑236 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                                                                                                                                                                                                                                     >  2:1282594/1‑151 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                                                                                                                                                                                                                                     <  1:1282594/151‑1 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACg                                                                                                                                                                                                                         <  1:543058/163‑1 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGAt                                                                                                                                             >  1:281611/1‑239 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCCTCCGATCATCGAt                                                                                                                                             >  1:862414/1‑239 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCAGCCCGACTAACTTTATCCGTCAGATCATCGAt                                                                                                                                             >  1:814684/1‑239 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTAAGGAATCCACGATGGGTGAGGCAGAAGCCCGCCCGCCTACCTGTATCCGTTGGATCATGGAt                                                                                                                                             >  1:77149/1‑239 (MQ=255)
                                                                                       gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCACCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACg                                                                                                                                                                                                                         >  2:543058/1‑163 (MQ=255)
                                                                                       gATGCCACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGCTGTCAGCCTGTCCCGCTTATAAGTCATACCCCGTTTTACATTGTTTACGCTTTGAGGAATCCACGGTGAGTGAGGCAAAAGCCCGCCCGACTAACTTTATCCTTCCGaacatcagta                                                                                                                                            >  2:998417/1‑235 (MQ=255)
                                                                                        gtggggtgCTTGTGCGTGGCATCCACAAATTGCATGCGGTTGAGATCTTCCCGGCCGCTTTTTTTTTCATCAATCATCCCCAGAATCCTTGTTAGTTTATCAATTTTAAAAAACTAACAGTTGGCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATg                                                                                                                                            <  2:77149/232‑1 (MQ=255)
                                                                                             aCGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGa                                                                                                                                        >  1:297941/1‑238 (MQ=255)
                                                                                                   acaggTCGCTTCCAACAATGACAAGCGTTGGAGTTTTTCTCTGCCCCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGc                                                                                                                                  <  2:682764/234‑1 (MQ=255)
                                                                                                     gCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCACCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCa                                                                                                                                <  1:1034046/238‑1 (MQ=255)
                                                                                                        tCGCATTCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTg                                                                                                                             <  1:978892/238‑1 (MQ=255)
                                                                                                        tCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGCCCGATCATCGTTGAAGATCTGGcccgagg                                                                                                                            >  1:733388/1‑234 (MQ=255)
                                                                                                              ccAAAAATGACCAGGGGTGGAGATCTTTTCTGCCGCTTTTTTTTTCATCAAACCACCCCAAAATCCTTTTTAGATTATAAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGAACATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGca                                                                                                                      <  2:733388/239‑1 (MQ=255)
                                                                                                                aagaaTGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGcaca                                                                                                                    <  1:464113/236‑1 (MQ=255)
                                                                                                                aaaaaTGACAAGCGGGTGAGTTTTTGTTGGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGcaca                                                                                                                    <  2:505527/236‑1 (MQ=255)
                                                                                                                  caaTGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGCCTAACTTTATCCGTCAGATCATCGTTGAAGATCTGGCCGGTGGTAAGcctgtt                                                                                                                  >  1:682764/1‑234 (MQ=255)
                                                                                                                   aaTGACAAGCGGTGGATATCTTCTCTGCCGTTTTTTTTTTCAGCAATCATCCCCATAATCTTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCAcacca                                                                                                                 <  1:1286607/239‑1 (MQ=255)
                                                                                                                          aGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGAAATCCACGATTAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCTTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGccaacaccagacc                                                                                                           >  1:1377389/1‑226 (MQ=255)
                                                                                                                                       ttctCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTTTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTTAGGAATCCCCGATGAGTGAGGCAGAAGCACGCCCGACCAACTTTATCCGTCAGATCATCGATGAAAATCTGCCCAGGGGTAACAAAACCACAGCAAAAACCCCTTTccc                                                                                              >  2:608747/1‑238 (MQ=255)
                                                                                                                                          ttggCCGCTTTTTTTTTCATCAATCCTCCCCAAAATCCTTGTTAGATTACCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGATTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCccgccg                                                                                          <  2:297941/236‑1 (MQ=255)
                                                                                                                                                gttttttttttCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGTCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCTAGTGGTAAGCACGCCCCAGTACACACCCGTTTCCCGCCGGAGCCGa                                                                                   <  1:998417/237‑1 (MQ=255)
                                                                                                                                                         ttCATCAATCATCCCCATAATCCTTGTTATATTAACAATGTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTAGACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTAt                                                                           <  1:608747/239‑1 (MQ=255)
                                                                                                                                                          taaaagagacagcccATAATCCTTGTTAGATTAGCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTAt                                                                           <  1:51579/226‑1 (MQ=255)
                                                                                                                                                                atcatcCCCATAATCCTTGTTAGATTACCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATCCGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCata                                                                    <  1:116295/239‑1 (MQ=255)
                                                                                                                                                                         aTAACCCTTTTTTGTTTACCAATTTTAAAAAACTAAAGGTTTTCAGCCTGTCCCGCTTAAAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATg                                                           <  2:281611/239‑1 (MQ=255)
                                                                                                                                                                                ttGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGt                                                                                                                              <  1:57668/165‑1 (MQ=255)
                                                                                                                                                                                ttGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGt                                                                                                                              >  2:57668/1‑165 (MQ=255)
                                                                                                                                                                                 tGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGCCTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGGGGTAAGCACACCACAGTCCACAACCGTTTCCCGCCGGAGCCGAAAGGCTACCTCCATATTg                                                                 >  2:51579/1‑225 (MQ=255)
                                                                                                                                                                                 tGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCCGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTACCCCCCGAACCCGAACGGCAATCTGCAAATTGGCCCTGCGaaaacc                                                   >  2:1252136/1‑236 (MQ=255)
                                                                                                                                                                                 tGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTCCATATTGGCCATGCGAAatct                                                   >  1:638306/1‑239 (MQ=255)
                                                                                                                                                                                  gTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCACATTGGCCATGCGAAatcta                                                  >  1:1055886/1‑239 (MQ=255)
                                                                                                                                                                                  gTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGAGGCCGAATGGCTATCTGCATATTGGCCATGCGACatcta                                                  >  1:839763/1‑239 (MQ=255)
                                                                                                                                                                                       aTTATCAATTTTAAAAAACTAACAGTTTTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTa                                                                                                                                                              <  2:200942/126‑1 (MQ=255)
                                                                                                                                                                                       aTTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTa                                                                                                                                                              >  1:200942/1‑126 (MQ=255)
                                                                                                                                                                                       aTTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAAGGGCTATCTGAATATTGGCCATGCGTAATCTATCTg                                              >  1:1268273/1‑238 (MQ=255)
                                                                                                                                                                                           tCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGa                                         >  2:362633/1‑239 (MQ=255)
                                                                                                                                                                                                              tgTTTTCAGCCGGTCCGGTTTATAAGATCACACGCCGTTATACGTTGTTTAGGTTTTGAGAAACCCAAGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCAGCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGa                      <  2:862414/238‑1 (MQ=255)
                                                                                                                                                                                                              ctttGTCAGCCTGTCCCGTTTATAAGATCATGCTCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGa                      <  2:441960/237‑1 (MQ=255)
                                                                                                                                                                                                              cgTTGTCAGCCTTCCCCGTTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAACCCACGATGAGGGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGa                      <  2:676579/238‑1 (MQ=255)
                                                                                                                                                                                                              aGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGa                      <  1:449588/239‑1 (MQ=255)
                                                                                                                                                                                                                 tGTCAGGCTTTCCCGCTTATAAGATCATACGCCGTTATCCGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGAATAACTTTATCCGTCAGATCATAGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGa                      <  1:867407/236‑1 (MQ=255)
                                                                                                                                                                                                                 tGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGa                      <  1:586318/236‑1 (MQ=255)
                                                                                                                                                                                                                  gTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACtat                  >  1:918111/1‑239 (MQ=255)
                                                                                                                                                                                                                  gTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATAATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGAAGACACCCGTTTCCCGCCGGAGCCGAAAGGCCATCTGCATATTGGCCATCAGAAATCTATCTGCCTGAACTTCGGGTTCGTCCAGGAcaca                  >  1:474699/1‑236 (MQ=255)
                                                                                                                                                                                                                    cAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGCCTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGATTGGCTATCTGCATATTGGCCATGCGACACCTATCTGCCTGAACTTCGGGATCGCCCAGGACtatca                >  1:195706/1‑237 (MQ=255)
                                                                                                                                                                                                                       ccTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAgg             <  1:102201/239‑1 (MQ=255)
                                                                                                                                                                                                                          gTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACAACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATTCGAAATCTATCTGCCTGAACTTCGGGATCCCCCAGGACTATAAAGGACa          >  2:1039542/1‑239 (MQ=255)
                                                                                                                                                                                                                           tCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGccct         >  1:441960/1‑237 (MQ=255)
                                                                                                                                                                                                                                 ttATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCCCGATGAGTGAGGCAGAAGCCCGCCCGAATAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGCCAGTGCAAc   <  1:35080/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tataAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGCAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGCCAGTGCAAcc  >  1:411837/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        
AGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGAGGCAGAAGCCCGCCCGACTAACTTTATCCGTCAGATCATCGATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGCCAGTGCAACC  >  NC_000913/705819‑706282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: