New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3972664 =128 (1.120)3 (0.030) 3/410 NT 2.4% coding (144/1068 nt) rffG dTDP‑glucose 4,6‑dehydratase
?NC_000913 3972682 = 120 (1.090)coding (162/1068 nt) rffG dTDP‑glucose 4,6‑dehydratase
Rejected: Frequency below/above cutoff threshold.

AACGGAACGCTGATTTTTTATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATATCAACTTTCTCAAAGGCAAAGCGCTCGCTTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3972905‑3972664
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgctttgcCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTT  >  NC_000913/3972682‑3972905
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
AACGGAACGCTGATTTTTTATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATATCACCTTTCTCAAAGGCAAAGCCCTCGCTT                                                                                                                                                                                                                                     >  1:146846/1‑239
                                                                                                                                                                  GCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATATCAACTTTCTCAAAGGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGGGTTTATTGAAACCAACATTGTCGGGACTTATAC                                                                   >  1:132063/1‑239
                                                                                                                                                                    TGATGCTCAGTTAAACCGCGTTCCATTTTGTCCCTATCGCATATATCAACTTTCTCAAGGGCAAAGCGAGCGCTTTGCCTTTGAGAAATTTGATATCTGGGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACA                                                                  <  2:132063/238‑1
                                                                                                                                                                       TGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATATCAACTTTCTCAAAGGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGGGTCATGCATCTGGCAGCCGAAAGCCAGGTTGACCGTTTTATTGACGGCCCGGAAGCGTTTATTGAAACCAAAATTGCTGGGCCTTttcgagcgg                                                              >  1:213660/1‑230
                                                                                                                                                                                                                             tccgccaggcgatgtgTTTCCCTTTGAAAAAGGTGATATCTGCGATCGGGACGAACTGGAACGGGTATTCACTGGGCATCAGCCAGACTTGGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCCATTGACGGCCCGGCAGCGTTTATTGAAACCAACATGGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCG         <  2:1152098/222‑1
                                                                                                                                                                                                                                   aagggtgcgtTTTGCCTTGGAGAAAGTTGATATCTGCGATCGGGCAGACCTGGCCCGGGTATTCACTGAGCATCAGCCAGGCTGTGTCATGCAGCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTT  <  2:247215/229‑1
                                                                                                                                                                                                                                          CGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTC                                                                              >  1:1151200/1‑156
                                                                                                                                                                                                                                          CGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGGATTCACTGAGCATCAGCCAGACTGGGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTC                                                                              <  2:1151200/156‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
AACGGAACGCTGATTTTTTATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATATCAACTTTCTCAAAGGCAAAGCGCTCGCTTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3972905‑3972664
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgctttgcCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACCAACATTGTCGGGACTTATACATTGCTTGAAGCGGCGCGGGCTTACTGGAATGCGCTGACGGAAGATAAAAAATCAGCGTTCCGTT  >  NC_000913/3972682‑3972905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.