Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 5,439 | A→C | 25.7% | H69P (CAC→CCC) | yaaX → | DUF2502 family putative periplasmic protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 5,439 | 0 | A | C | 25.7% | 117.5 / 4.1 | 71 | H69P (CAC→CCC) | yaaX | DUF2502 family putative periplasmic protein |
Reads supporting (aligned to +/- strand): ref base A (29/20); new base C (7/15); total (36/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.20e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGT > NC_000913/5201‑5653 | aCCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCa < 2:298635/239‑1 (MQ=255) tAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTac > 1:899400/1‑239 (MQ=255) aaCGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTac > 1:768666/1‑238 (MQ=255) gCAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCCCTGGCACCTACAC‑GGa > 1:218043/1‑238 (MQ=255) cAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAAATACATTATGAATGGGCA‑GGCAATCGGTGGACACTACAC‑GGAc > 1:42268/1‑238 (MQ=255) aGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGACTGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CAccgcc > 1:912166/1‑238 (MQ=255) tAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACGGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCGCGGCTGGTGGAACCAAAATTATGAATGGCGG‑GGCAATGCCTGGCCCCACAAC‑GGAACGCCGC‑CCCcgccgc > 2:554169/1‑238 (MQ=255) tAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGCATGGCGA‑GGCAAGCGCTGGCCCCTCCAC‑GGACCCCCCC‑CCCccccgc > 2:459826/1‑238 (MQ=255) tAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCGGGCCCCTACAC‑GGACCGCCGC‑CAccgcccc > 1:680497/1‑236 (MQ=255) tAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGGGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACcgccgc > 1:1316165/1‑238 (MQ=255) tAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATAGTGGCTATTAAGTGGATGGAGGTCACTGACCGG‑ACACCGCCTGGGGGGAAAAAAATTATGATTGGCCG‑GGCAAACGGCGGCACCTACAC‑GGACCGCCGC‑CACgcccgc > 2:983682/1‑238 (MQ=255) accctggAAAAAGATTGCAATTTATCGGCCCCCCATTTCCCCTGGTTC‑GGGGCGCCCCCAGGGCAGCCAGGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATGGGGGATCGTGATAATCGTGGCTATTACGGGGATGGAGGTCACGGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCgcgc < 2:899400/233‑1 (MQ=255) cggagatgtggaaaagagacagaTCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCgcgc < 1:539110/216‑1 (MQ=255) cctttaaaaaaGA‑TGAAATCTTCCGT‑CCCTCCCTTTCCCTGGGTCGTGGCGCCTCCCATGGCAGACCAGGCTGGGGAAATTACGTTAGCCCAGTCAATAAAATTACAAATAGGCGTGCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGAAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGcca < 2:1209427/233‑1 (MQ=255) ccTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGAAAGGCGATCGTGGTAATCGTGGCTATTACTGGGATGGAGGTCACTGGCCCG‑ACCACGGCTGGCGG‑AACAAAACTATTGATGGTGAGGGGAAAACCTGGCACCCACAC‑GAACcccccc‑ccccccgccggc > 2:956760/1‑230 (MQ=255) ttaaaaaaGA‑TGCATTCTGTGCTGCCCCCACTTTCCCTGTGTC‑TGGCCCCTCCCAGGGCAGCAAAGGCTGCGGAAATTACGTTAGTCCCGGCAGGAAAATTACAGATAGGCGGTCGTGATAATCGTTGCTATTACTGGGATGGAGGTCACGGGGGCG‑ACCACGGCGGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGccacca < 2:695410/236‑1 (MQ=255) tgAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACACAATTATGAATGGCGA‑GGCAATCACTGGCACCTCCAC‑GGACCCCCCC‑CACCCCCCCCcccccaa > 2:1000318/1‑238 (MQ=255) aa‑TGCAATTCTTCGTCCCCGCACTTCCCCTGGTTT‑GGGCCGCCCCCATGGCAGCCCGGGCTGGGAAAATTACGTTAGTCCCGTCAGAAAAATTACAGAGAGGCGATCGTGAAAACCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACACCATTATGAATGGCGA‑GGCAATCGCTGGCCCCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAg < 2:768666/238‑1 (MQ=255) tGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGc > 1:271285/1‑238 (MQ=255) gCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATACGAAAGct > 1:941051/1‑238 (MQ=255) aagcTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGctcc < 1:806100/235‑1 (MQ=255) ctttttCGTTCTCCCTCTTTCCTTGGTTT‑TGGTCGCCCCAAGGGCACACCAGGCTGGGGAAATTCCGTTAGTCCGGTCAGTAAAATTACAGAAGGGCGATCGTGATAATCGTGGCTATTACTGGGAGGGAGGTCACTGGCGCG‑CCCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCCCCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGctcctc < 2:1316165/234‑1 (MQ=255) aCTCGCCTTTTCCCTGGTTC‑GGGGGCCCCCAATGGCAGCAAAGGCGGCGGAAATTCGGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTAGGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGatcat < 1:1190131/238‑1 (MQ=255) cTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCCA‑GGCAATCGCTGGACCCAACAC‑GGACCCCCGCACACGACCGCCCCCCCaaaaaaaacaccctcaatacaaaa > 2:73833/1‑223 (MQ=255) cTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGGGGAAACAACATTATGAAAGGCGT‑GGCAATCACGGGCCACTACAC‑GGACCCCCGC‑CACCACCGGACCCCCATAAGAAAgaacacgataataatca > 2:95736/1‑223 (MQ=255) tCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAACTTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GCCAATCGCCTGCCCCTACAC‑GGACCGCCCC‑CACCCCCCCCGCCCCATAAAACAACCCCTCATCaacatacc > 2:698294/1‑236 (MQ=255) gCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCAGAATCATCAcg > 1:754940/1‑238 (MQ=255) aCTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CCCCGCCGCGCCCCCATAAGAAAGCTCCTCATGATCATCAcggcg > 1:341469/1‑239 (MQ=255) aCTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGAGGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCTA‑AGGAATCGCTGGCACCTACAC‑GGCCCACCGC‑CCCCCCCCGGCCCCCCTAAAAAAACACCCCATCACCAACAcggcg > 2:1344023/1‑239 (MQ=255) gTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTAGTAATGGCGA‑GGCACACGCTGGCACCCACAC‑GGACCGCCCC‑CACCCCACCCCCACCCAAAAAAAGCCCCCCAtctccacaaccgccggcccgagc > 2:195104/1‑216 (MQ=255) gTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTCAAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCATGGTCCCGGcca > 1:568921/1‑237 (MQ=255) cGCCCCCATGGCAGACCAGGCTGCGAAAATTACGTTAGCCCCGGCAGTAAAATTACAGAAAGGCAATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGCCCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCaaa < 1:643941/238‑1 (MQ=255) cTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCCAGGGCCCAGCAAACa > 1:1149783/1‑238 (MQ=255) cTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑AACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCCGGCaaaaa > 1:930589/1‑236 (MQ=255) cagcaCAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTCACC‑GGACCGCCGC‑CACCCCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCACGGTCCAGGAAAACAACAcccccca > 2:1046722/1‑234 (MQ=255) gcaCAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑AGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCCCCGCGCCACCATAAGAAAGCCCCTCATGATCATCACGGCGGGCACGGTCCCGGCcaaaagacacactcaaa > 1:451967/1‑225 (MQ=255) acaGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCCCCATAAGAAAGCTCCTCATGATCATCACGGCGGGCACTGTCCACGCAAACATCACCGCCAAAAGa > 1:324268/1‑239 (MQ=255) gCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCAGGGGTCAGGCCAACACCACCCCCAAATCACaaa > 1:1106669/1‑239 (MQ=255) tCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCCTTGTCCCGGCAAAAATCACCCCTAAATGACAAAATCCGGGGTACAATCCGGCATTc > 1:372738/1‑238 (MQ=255) tAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTGCCGGCAAACATCACCGCTAAATGACCAAAGCCGGGGAACAAACCCGCCTTCAggc > 1:1123087/1‑236 (MQ=255) tacggtagggCGATCGTTATAATCGTGTCTTTTACGGGGGTGGGGGCCACTGGCGGG‑ACCACGGTGGGGGGAACAAACATTATGAATGGCGG‑GGCAATCGCTGGCCCCTACAC‑GGTCCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑Tg < 2:271285/231‑1 (MQ=255) tAATCGGGGCTTTTCCGGGGATGGAGGTCCCTGGCGCG‑ACCCCGGCTGGTGGAAACAACCTTATGAAGTGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTAt < 1:1248190/239‑1 (MQ=255) atagcggtgtattgacggggATGGAGGTCCGGCGCGCC‑GCCAGGGGTGGCAGAAACAACATTAGGAAGGGCGA‑GGCAATCGCTGGCCCCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTAt < 2:912166/222‑1 (MQ=255) aaagcGTGGCTTTTCCGGGGATGGAGGTCACGGGCGCG‑ACCGCGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCCCCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTAt < 1:554169/235‑1 (MQ=255) ttgataaaaagagagggggggccACGGGCCCG‑CCCCCGGCTGGTGGAAAAAACATTAAGAATGGCGA‑GGCCATCGGTGGCACCTACAC‑GGACCGCCCC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGcc < 2:452316/217‑1 (MQ=255) ggCTTTTCCTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGcc < 1:459826/238‑1 (MQ=255) ggCTTTTCCGGGGATGGAGGTC‑CTGGGCCGCACCACGGCTGGTGGAAACAACATTATGAAGGGCGA‑GGCAATCGCTGGCCCCCACCC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGcc < 1:1231706/238‑1 (MQ=255) ggCTTTTACTGGGATGGAGGTCACTGGCGCC‑GCCCCGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACCC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGcc < 1:73833/238‑1 (MQ=255) tttccggggggggggtgcccggggCGCG‑‑CCACGGGGGGGGAAAACAAGTTTATGAATGGCGA‑GGCAATCGCTGGCCCCTACGC‑GGCCCGCCGC‑CCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGt < 2:1149783/217‑1 (MQ=255) acggggATGGGGTGCACTGGCGCG‑ACCACGGCTGGGGGAAACAACATTAGGAAGGGCAG‑GGAAATCGCTGGCACCTACAC‑GGCCCGCCGC‑CACCGCCGGGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa < 1:793615/236‑1 (MQ=255) aCTGGGTGGGAGGTCAGTGGCCAG‑ACCACGGCTGGGGAAAACAACATTATGAAGGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa < 1:1000318/239‑1 (MQ=255) tggggtggggGTCCCGGGCGCG‑CCCGCGGCGGGGGGAACAAACATTTTGGATGGCAG‑GGCAATCGCTGGCCCCT‑CACGGGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt < 2:1236930/239‑1 (MQ=255) tGGGATGGAGGCTACTGGCGCC‑ACCAGGGCTGGTGGAAACAACATTATGAAGGGCGA‑GGAAAGCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt < 1:716152/239‑1 (MQ=255) ggggttggggtgcccTGGGGCG‑ACCCCGGCTGGGGGAACCCACATTTAGAAGGGCGG‑GGCAATCGCTGGCCCCTACAC‑GGCCCGCCGC‑CCCCGCCGGGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt < 2:320473/225‑1 (MQ=255) aTGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGCAAg > 2:1170346/1‑108 (MQ=255) aTGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAg < 1:1170346/108‑1 (MQ=255) ggAGGTCCCTGGGGCG‑CCCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa < 1:1046722/238‑1 (MQ=255) gAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGGAAACATCACCGCTAAATGACAAATGCCGGGGAACAAACCGGCATTCAGCGCCTGA‑TTCGACGCCGGGGCGGCTTTTCAGGCCCAAGGTAATTTTGCaaa > 1:1164901/1‑238 (MQ=255) gtGGGGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGG‑GGCAATCGCTGGCCCCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGa < 1:378576/238‑1 (MQ=255) ctggcacacccgcggtgggggaaaaCAACATTATGAAGGGCGA‑GGCAATCGCTGGCCACTCCAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGa < 1:195104/217‑1 (MQ=255) gcgcG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCCAGCAAACATCCCCGCTAAATTAAAAAAGCCCGGGAACAAACCGGCATTCAACGCCTGA‑TGCGACCCTGGCCGGCCTTATCAGGCCTACGCTAATTCTGcccggctcttttac > 1:533756/1‑226 (MQ=255) gcgcG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTCAATCACAAATGCCGGGGAACAATCCGGCATTCAGCGCCTGA‑TGCGGCGCTGGCGCGGCTTATCAGGCCTACGGTAAtt > 1:452316/1‑222 (MQ=255) ggccACGGTTTGGGGAAACAACATTAGGAATGGCAA‑GGCAATCGCTGGCCCCTACCC‑GGCCCGCCGC‑CACCGCCGGGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa < 1:954073/237‑1 (MQ=255) gccctgggtgggtggAAAAAACATTAGAAATGGGGG‑GGCAATCGCGGGCCCCTCACC‑GGCCCGCCCC‑CCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa < 2:372738/229‑1 (MQ=255) ccgccGGTTGGTCGAAACAAAATGATGAAGGGCGA‑GGCAATCGCTGGCACCTCCAC‑GGACCGCCGA‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa < 1:1188737/234‑1 (MQ=255) cccgggCCGGTGGAAACAAAATTAGGAATGGCGA‑GGCAATCGGTGGCCCCCACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATg < 1:8789/235‑1 (MQ=255) tggAAACACCATTAAGAATGGCGA‑GGCAATCGCTGGCCCCTACCC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCCCGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAg < 1:476628/238‑1 (MQ=255) ggAAACAACATTAAGAATGGCTA‑TGCAATCGCTGGCACCCACCC‑GGACCCCCGC‑CACCGCCGCGACACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg < 1:962449/238‑1 (MQ=255) caTTATGAATGGCGA‑GGCAACCGCTGGCACCTACCC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGcc < 1:97259/157‑1 (MQ=255) caTTAAGAATGGCGA‑GGCAATCGCTGGCACCTCCAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCCCCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt < 1:190891/197‑1 (MQ=255) aTGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGTATGGGCCAGGGCAAAATCACCCCTCAAAAACAAAACCCGGGGAACAAACAAGCATTCAGCCACCCA‑TACGACACCGGCGCGAGCTTTCAGGCCTCCTCGAATTCTGCACTATATTTAATACGCATAACTTTGTTGGccagaaaaaaaga > 2:252355/1‑227 (MQ=255) tGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCAGGCAAACATCCCCGCTCAAAAACAAATGACGGGGAACAATCCCGCATTCAGACCCGCACTGCG‑CGCTGGGGCGCCTTTTCAGGACCCCGTTGATTTTGCAATAAATTTAATCTGCATGCTTTTTTTGGCAGGAGAAggggg > 2:925635/1‑235 (MQ=255) | ACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA‑TGCAATCTATCGTACTCGCACTTTCCCTGGTTC‑TGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCG‑ACCACGGCTGGTGGAAACAACATTATGAATGGCGA‑GGCAATCGCTGGCACCTACAC‑GGACCGCCGC‑CACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGA‑TGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGT > NC_000913/5201‑5653 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |