Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 5,439 A→C 25.7% H69P (CAC→CCC)  yaaX → DUF2502 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,4390AC25.7% 117.5 / 4.1 71H69P (CAC→CCC) yaaXDUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (29/20);  new base C (7/15);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 4.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGT  >  NC_000913/5201‑5653
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                         
aCCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCa                                                                                                                                                                                                                           <  2:298635/239‑1 (MQ=255)
     tAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTac                                                                                                                                                                                                                      >  1:899400/1‑239 (MQ=255)
      aaCGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTac                                                                                                                                                                                                                      >  1:768666/1‑238 (MQ=255)
           gCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCCCTGGCACCTACACGGa                                                                                                                                                                                                                >  1:218043/1‑238 (MQ=255)
            cAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAAATACATTATGAATGGGCAGGCAATCGGTGGACACTACACGGAc                                                                                                                                                                                                               >  1:42268/1‑238 (MQ=255)
                         aGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGACTGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCAccgcc                                                                                                                                                                                                 >  1:912166/1‑238 (MQ=255)
                           tAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACGGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCGCGGCTGGTGGAACCAAAATTATGAATGGCGGGGCAATGCCTGGCCCCACAACGGAACGCCGCCCCcgccgc                                                                                                                                                                                               >  2:554169/1‑238 (MQ=255)
                           tAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGCATGGCGAGGCAAGCGCTGGCCCCTCCACGGACCCCCCCCCCccccgc                                                                                                                                                                                               >  2:459826/1‑238 (MQ=255)
                           tAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCGGGCCCCTACACGGACCGCCGCCAccgcccc                                                                                                                                                                                               >  1:680497/1‑236 (MQ=255)
                           tAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGGGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACcgccgc                                                                                                                                                                                               >  1:1316165/1‑238 (MQ=255)
                           tAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATAGTGGCTATTAAGTGGATGGAGGTCACTGACCGGACACCGCCTGGGGGGAAAAAAATTATGATTGGCCGGGCAAACGGCGGCACCTACACGGACCGCCGCCACgcccgc                                                                                                                                                                                               >  2:983682/1‑238 (MQ=255)
                             accctggAAAAAGATTGCAATTTATCGGCCCCCCATTTCCCCTGGTTCGGGGCGCCCCCAGGGCAGCCAGGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATGGGGGATCGTGATAATCGTGGCTATTACGGGGATGGAGGTCACGGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCgcgc                                                                                                                                                                                             <  2:899400/233‑1 (MQ=255)
                              cggagatgtggaaaagagacagaTCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCgcgc                                                                                                                                                                                             <  1:539110/216‑1 (MQ=255)
                              cctttaaaaaaGATGAAATCTTCCGTCCCTCCCTTTCCCTGGGTCGTGGCGCCTCCCATGGCAGACCAGGCTGGGGAAATTACGTTAGCCCAGTCAATAAAATTACAAATAGGCGTGCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGAAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGcca                                                                                                                                                                                           <  2:1209427/233‑1 (MQ=255)
                              ccTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGAAAGGCGATCGTGGTAATCGTGGCTATTACTGGGATGGAGGTCACTGGCCCGACCACGGCTGGCGGAACAAAACTATTGATGGTGAGGGGAAAACCTGGCACCCACACGAACccccccccccccgccggc                                                                                                                                                                                            >  2:956760/1‑230 (MQ=255)
                                 ttaaaaaaGATGCATTCTGTGCTGCCCCCACTTTCCCTGTGTCTGGCCCCTCCCAGGGCAGCAAAGGCTGCGGAAATTACGTTAGTCCCGGCAGGAAAATTACAGATAGGCGGTCGTGATAATCGTTGCTATTACTGGGATGGAGGTCACGGGGGCGACCACGGCGGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGccacca                                                                                                                                                                                        <  2:695410/236‑1 (MQ=255)
                                  tgAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACACAATTATGAATGGCGAGGCAATCACTGGCACCTCCACGGACCCCCCCCACCCCCCCCcccccaa                                                                                                                                                                                       >  2:1000318/1‑238 (MQ=255)
                                         aaTGCAATTCTTCGTCCCCGCACTTCCCCTGGTTTGGGCCGCCCCCATGGCAGCCCGGGCTGGGAAAATTACGTTAGTCCCGTCAGAAAAATTACAGAGAGGCGATCGTGAAAACCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACACCATTATGAATGGCGAGGCAATCGCTGGCCCCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAg                                                                                                                                                                                <  2:768666/238‑1 (MQ=255)
                                            tGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGc                                                                                                                                                                               >  1:271285/1‑238 (MQ=255)
                                             gCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATACGAAAGct                                                                                                                                                                              >  1:941051/1‑238 (MQ=255)
                                               aagcTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGctcc                                                                                                                                                                            <  1:806100/235‑1 (MQ=255)
                                                ctttttCGTTCTCCCTCTTTCCTTGGTTTTGGTCGCCCCAAGGGCACACCAGGCTGGGGAAATTCCGTTAGTCCGGTCAGTAAAATTACAGAAGGGCGATCGTGATAATCGTGGCTATTACTGGGAGGGAGGTCACTGGCGCGCCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCCCCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGctcctc                                                                                                                                                                          <  2:1316165/234‑1 (MQ=255)
                                                         aCTCGCCTTTTCCCTGGTTCGGGGGCCCCCAATGGCAGCAAAGGCGGCGGAAATTCGGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTAGGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGatcat                                                                                                                                                                  <  1:1190131/238‑1 (MQ=255)
                                                          cTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCCAGGCAATCGCTGGACCCAACACGGACCCCCGCACACGACCGCCCCCCCaaaaaaaacaccctcaatacaaaa                                                                                                                                                                 >  2:73833/1‑223 (MQ=255)
                                                          cTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGGGGAAACAACATTATGAAAGGCGTGGCAATCACGGGCCACTACACGGACCCCCGCCACCACCGGACCCCCATAAGAAAgaacacgataataatca                                                                                                                                                                >  2:95736/1‑223 (MQ=255)
                                                           tCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAACTTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGCCAATCGCCTGCCCCTACACGGACCGCCCCCACCCCCCCCGCCCCATAAAACAACCCCTCATCaacatacc                                                                                                                                                               >  2:698294/1‑236 (MQ=255)
                                                             gCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCAGAATCATCAcg                                                                                                                                                              >  1:754940/1‑238 (MQ=255)
                                                               aCTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCCCCGCCGCGCCCCCATAAGAAAGCTCCTCATGATCATCAcggcg                                                                                                                                                           >  1:341469/1‑239 (MQ=255)
                                                               aCTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGAGGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCTAAGGAATCGCTGGCACCTACACGGCCCACCGCCCCCCCCCGGCCCCCCTAAAAAAACACCCCATCACCAACAcggcg                                                                                                                                                           >  2:1344023/1‑239 (MQ=255)
                                                                         gTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTAGTAATGGCGAGGCACACGCTGGCACCCACACGGACCGCCCCCACCCCACCCCCACCCAAAAAAAGCCCCCCAtctccacaaccgccggcccgagc                                                                                                                                                  >  2:195104/1‑216 (MQ=255)
                                                                                gTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTCAACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCATGGTCCCGGcca                                                                                                                                           >  1:568921/1‑237 (MQ=255)
                                                                                  cGCCCCCATGGCAGACCAGGCTGCGAAAATTACGTTAGCCCCGGCAGTAAAATTACAGAAAGGCAATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGCCCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCaaa                                                                                                                                          <  1:643941/238‑1 (MQ=255)
                                                                                    cTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCCAGGGCCCAGCAAACa                                                                                                                                        >  1:1149783/1‑238 (MQ=255)
                                                                                    cTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCAACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCCGGCaaaaa                                                                                                                                        >  1:930589/1‑236 (MQ=255)
                                                                                             cagcaCAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTCACCGGACCGCCGCCACCCCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCACGGTCCAGGAAAACAACAcccccca                                                                                                                              >  2:1046722/1‑234 (MQ=255)
                                                                                               gcaCAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAAGCAATCGCTGGCACCTACACGGACCGCCGCCACCCCCGCGCCACCATAAGAAAGCCCCTCATGATCATCACGGCGGGCACGGTCCCGGCcaaaagacacactcaaa                                                                                                                            >  1:451967/1‑225 (MQ=255)
                                                                                                 acaGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCCCCATAAGAAAGCTCCTCATGATCATCACGGCGGGCACTGTCCACGCAAACATCACCGCCAAAAGa                                                                                                                          >  1:324268/1‑239 (MQ=255)
                                                                                                     gCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCAGGGGTCAGGCCAACACCACCCCCAAATCACaaa                                                                                                                      >  1:1106669/1‑239 (MQ=255)
                                                                                                                             tCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCCTTGTCCCGGCAAAAATCACCCCTAAATGACAAAATCCGGGGTACAATCCGGCATTc                                                                                               >  1:372738/1‑238 (MQ=255)
                                                                                                                                 tAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTGCCGGCAAACATCACCGCTAAATGACCAAAGCCGGGGAACAAACCCGCCTTCAggc                                                                                           >  1:1123087/1‑236 (MQ=255)
                                                                                                                                       tacggtagggCGATCGTTATAATCGTGTCTTTTACGGGGGTGGGGGCCACTGGCGGGACCACGGTGGGGGGAACAAACATTATGAATGGCGGGGCAATCGCTGGCCCCTACACGGTCCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATg                                                                                   <  2:271285/231‑1 (MQ=255)
                                                                                                                                                          tAATCGGGGCTTTTCCGGGGATGGAGGTCCCTGGCGCGACCCCGGCTGGTGGAAACAACCTTATGAAGTGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                <  1:1248190/239‑1 (MQ=255)
                                                                                                                                                          atagcggtgtattgacggggATGGAGGTCCGGCGCGCCGCCAGGGGTGGCAGAAACAACATTAGGAAGGGCGAGGCAATCGCTGGCCCCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                <  2:912166/222‑1 (MQ=255)
                                                                                                                                                          aaagcGTGGCTTTTCCGGGGATGGAGGTCACGGGCGCGACCGCGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCCCCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt                                                                <  1:554169/235‑1 (MQ=255)
                                                                                                                                                                ttgataaaaagagagggggggccACGGGCCCGCCCCCGGCTGGTGGAAAAAACATTAAGAATGGCGAGGCCATCGGTGGCACCTACACGGACCGCCCCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                          <  2:452316/217‑1 (MQ=255)
                                                                                                                                                                 ggCTTTTCCTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                          <  1:459826/238‑1 (MQ=255)
                                                                                                                                                                 ggCTTTTCCGGGGATGGAGGTCCTGGGCCGCACCACGGCTGGTGGAAACAACATTATGAAGGGCGAGGCAATCGCTGGCCCCCACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                          <  1:1231706/238‑1 (MQ=255)
                                                                                                                                                                 ggCTTTTACTGGGATGGAGGTCACTGGCGCCGCCCCGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                          <  1:73833/238‑1 (MQ=255)
                                                                                                                                                                    tttccggggggggggtgcccggggCGCGCCACGGGGGGGGAAAACAAGTTTATGAATGGCGAGGCAATCGCTGGCCCCTACGCGGCCCGCCGCCCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGt                                                     <  2:1149783/217‑1 (MQ=255)
                                                                                                                                                                        acggggATGGGGTGCACTGGCGCGACCACGGCTGGGGGAAACAACATTAGGAAGGGCAGGGAAATCGCTGGCACCTACACGGCCCGCCGCCACCGCCGGGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa                                                  <  1:793615/236‑1 (MQ=255)
                                                                                                                                                                        aCTGGGTGGGAGGTCAGTGGCCAGACCACGGCTGGGGAAAACAACATTATGAAGGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa                                                  <  1:1000318/239‑1 (MQ=255)
                                                                                                                                                                          tggggtggggGTCCCGGGCGCGCCCGCGGCGGGGGGAACAAACATTTTGGATGGCAGGGCAATCGCTGGCCCCTCACGGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt                                                <  2:1236930/239‑1 (MQ=255)
                                                                                                                                                                          tGGGATGGAGGCTACTGGCGCCACCAGGGCTGGTGGAAACAACATTATGAAGGGCGAGGAAAGCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt                                                <  1:716152/239‑1 (MQ=255)
                                                                                                                                                                          ggggttggggtgcccTGGGGCGACCCCGGCTGGGGGAACCCACATTTAGAAGGGCGGGGCAATCGCTGGCCCCTACACGGCCCGCCGCCCCCGCCGGGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt                                                <  2:320473/225‑1 (MQ=255)
                                                                                                                                                                              aTGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGCAAg                                                                                                                                                                                >  2:1170346/1‑108 (MQ=255)
                                                                                                                                                                              aTGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAg                                                                                                                                                                                <  1:1170346/108‑1 (MQ=255)
                                                                                                                                                                                ggAGGTCCCTGGGGCGCCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCa                                           <  1:1046722/238‑1 (MQ=255)
                                                                                                                                                                                 gAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGGAAACATCACCGCTAAATGACAAATGCCGGGGAACAAACCGGCATTCAGCGCCTGATTCGACGCCGGGGCGGCTTTTCAGGCCCAAGGTAATTTTGCaaa                                         >  1:1164901/1‑238 (MQ=255)
                                                                                                                                                                                        gtGGGGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGGGGCAATCGCTGGCCCCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGa                                  <  1:378576/238‑1 (MQ=255)
                                                                                                                                                                                         ctggcacacccgcggtgggggaaaaCAACATTATGAAGGGCGAGGCAATCGCTGGCCACTCCACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGa                                  <  1:195104/217‑1 (MQ=255)
                                                                                                                                                                                           gcgcGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCCAGCAAACATCCCCGCTAAATTAAAAAAGCCCGGGAACAAACCGGCATTCAACGCCTGATGCGACCCTGGCCGGCCTTATCAGGCCTACGCTAATTCTGcccggctcttttac                               >  1:533756/1‑226 (MQ=255)
                                                                                                                                                                                           gcgcGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTCAATCACAAATGCCGGGGAACAATCCGGCATTCAGCGCCTGATGCGGCGCTGGCGCGGCTTATCAGGCCTACGGTAAtt                                                >  1:452316/1‑222 (MQ=255)
                                                                                                                                                                                                ggccACGGTTTGGGGAAACAACATTAGGAATGGCAAGGCAATCGCTGGCCCCTACCCGGCCCGCCGCCACCGCCGGGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa                           <  1:954073/237‑1 (MQ=255)
                                                                                                                                                                                                gccctgggtgggtggAAAAAACATTAGAAATGGGGGGGCAATCGCGGGCCCCTCACCGGCCCGCCCCCCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa                           <  2:372738/229‑1 (MQ=255)
                                                                                                                                                                                                 ccgccGGTTGGTCGAAACAAAATGATGAAGGGCGAGGCAATCGCTGGCACCTCCACGGACCGCCGACACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa                           <  1:1188737/234‑1 (MQ=255)
                                                                                                                                                                                                  cccgggCCGGTGGAAACAAAATTAGGAATGGCGAGGCAATCGGTGGCCCCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATg                         <  1:8789/235‑1 (MQ=255)
                                                                                                                                                                                                            tggAAACACCATTAAGAATGGCGAGGCAATCGCTGGCCCCTACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCCCGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAg                <  1:476628/238‑1 (MQ=255)
                                                                                                                                                                                                             ggAAACAACATTAAGAATGGCTATGCAATCGCTGGCACCCACCCGGACCCCCGCCACCGCCGCGACACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg               <  1:962449/238‑1 (MQ=255)
                                                                                                                                                                                                                     caTTATGAATGGCGAGGCAACCGCTGGCACCTACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGcc                                                                                         <  1:97259/157‑1 (MQ=255)
                                                                                                                                                                                                                     caTTAAGAATGGCGAGGCAATCGCTGGCACCTCCACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCCCCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAAtt                                                <  1:190891/197‑1 (MQ=255)
                                                                                                                                                                                                                         aTGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGTATGGGCCAGGGCAAAATCACCCCTCAAAAACAAAACCCGGGGAACAAACAAGCATTCAGCCACCCATACGACACCGGCGCGAGCTTTCAGGCCTCCTCGAATTCTGCACTATATTTAATACGCATAACTTTGTTGGccagaaaaaaaga  >  2:252355/1‑227 (MQ=255)
                                                                                                                                                                                                                          tGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCAGGCAAACATCCCCGCTCAAAAACAAATGACGGGGAACAATCCCGCATTCAGACCCGCACTGCGCGCTGGGGCGCCTTTTCAGGACCCCGTTGATTTTGCAATAAATTTAATCTGCATGCTTTTTTTGGCAGGAGAAggggg  >  2:925635/1‑235 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                         
ACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGT  >  NC_000913/5201‑5653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: