BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,047,043	T→A	11.0%	Y702* (TAT→TAA)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,047,043	0	T	A	11.0%	290.6 / 2.8	109	Y702* (TAT→TAA)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (64/28); new base A (5/9); total (70/39)
Fisher's exact test for biased strand distribution p-value = 1.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTAT  >  NC_000913/2046806‑2047281

cggggtgAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCtata                                                                                                                                                                                                                                               <  1:1264912/236‑1 (MQ=255)

  tggtgAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATAc                                                                                                                                                                                                                                              >  1:8430/1‑238 (MQ=255)

     taaaTATCATTTCTGTTTCGTCATCCCGACCTCACTCGACAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGATAAAACGGAGAGATGAAAATGACAAA‑CCTGTTAAGGAAAAAAAACAGAAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGc                                                                                                                                                                                                                                           <  1:347366/236‑1 (MQ=255)

      aaaTTTCATTTCTTTTTCGCCAACCCGAACTCACTCGTAAATTAAGATTGATAAGGACCGTTATCGCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGAAACTAAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGACTCACTACAGACTGAAAAGAAACCGCAGA‑GGGCGTCTATA‑AGGCGCCCTATACCGCCt                                                                                                                                                                                                                                         <  1:1232770/238‑1 (MQ=255)

      aaaTATCTTTTTTTTTTCGTCACCCCAAACTCACTGGTCAATTAAGATTGATAAGGACGTTTATCCCCCCGGCATTCCTATCGGGGTGACGGTAAAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAACCCGCAGA‑TGGCGTCTATA‑AGGCGCCCTATACCGCCt                                                                                                                                                                                                                                         <  1:78612/238‑1 (MQ=255)

        atatCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGGCTATA‑AGGCGACCAAAAACGccaaa                                                                                                                                                                                                                                       >  2:1308310/1‑236 (MQ=255)

             tttttttttccgccaccccGACCTCACTCATAAATAAAAATTGAAAAGAACCTTTATCTCCCCGGAAATCTAAGCGAGGTGACGGAAGAATGAAGAGAAAAAAATGACAAA‑CCTGTAAAGGAAAAAAAACAGCAACTGAAAACAGCAGAAAGCATCGAA‑AACGTGAAACCAGGAGTCCCTACAGACTGAAAAGAAACCGCAGA‑GGGCGTCAATA‑AGGCGACCTATACCGCCTATACCaa                                                                                                                                                                                                                                  <  2:1072919/223‑1 (MQ=255)

              tttCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCaaa                                                                                                                                                                                                                                 >  1:482911/1‑239 (MQ=255)

              tttCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTAAGCATCGCC‑AACGTGAAACCAGGAGACACTACAAACTGGAAAGAAACCCCCGA‑TGGCGTCATAA‑AGGCGCCCCAAACCGctgtcccttta                                                                                                                                                                                                                                 >  2:836051/1‑229 (MQ=255)

                 cTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGCGGACCTATACCGCCTATACCAAAGGc                                                                                                                                                                                                                              >  1:1359790/1‑239 (MQ=255)

                   gTTTCGTCATCCCGACCTCACGCGTCAATTAAGATTGATAAGGCCCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGAAGAACTGAGAGATGAAAAGGAAAAA‑CCTGTTAGGGAACAAAAACAGCAACTGAATAACGCAGACAGCATCGAC‑AACGGAAACCCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTAAA‑AGGCGACCTATACCGCCTATACCAAAGGCAg                                                                                                                                                                                                                            <  1:843348/239‑1 (MQ=255)

                    tttCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGt                                                                                                                                                                                                                           >  1:1229299/1‑239 (MQ=255)

                      tCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACAAGGGTGCACTACAGACTGGAAAGAAACAGCACA‑TGGCGTCTATA‑AAGCGCCCTACACCCCCTATAACAAAGGCAGTga                                                                                                                                                                                                                         >  2:720395/1‑238 (MQ=255)

                       cGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTggc                                                                                                                                                                                                                        >  1:663220/1‑238 (MQ=255)

                         tCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCCCCTATACCAAAAGCAGGGGACt                                                                                                                                                                                                                      >  2:326157/1‑239 (MQ=255)

                         ccaccccGACCTCATCTGTAAATTAAGTTTGAAAAGGACCGTTTTCTCGCCGGCAATCCTAGCGAGGGGACGGAAAAACGGAGAATAGAAAATGCAAAA‑CCTGTTAAGGAAAAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACGGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACt                                                                                                                                                                                                                      <  2:336053/235‑1 (MQ=255)

                                      actcGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCCCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTa                                                                                                                                                                                                         >  1:181875/1‑239 (MQ=255)

                                      actcGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGACACTACAGACTGGAAAAAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACGGCCTATACCAACGGCAGTGGACTTACTGCGCAGCTa                                                                                                                                                                                                         >  1:649997/1‑239 (MQ=255)

                                       tccGTAAATAAAGATTGATAAGGACCGTTATCTCCCGGGCAATCCTACCGAGGTGACGGTAAAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACGGAATAACGCAGTCAGCATCGAA‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAt                                                                                                                                                                                                        <  1:326157/237‑1 (MQ=255)

                                       atcttaaaTTAAAATTGAAAAGTACCGTTATCTTCCGGGCAATCCTAACGAGGTGACGGTAGAATTGAGAGATGAAAATGACAAC‑CGTGTTAAGTAACAAAAACAGCAACTGAATAACGCCGTCAGCATCTAA‑AACGTGAAACCAGGAGTCACTACAGCCTGAAAAGAAACCGCAGT‑TGGCGTCTATA‑AGGCAACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTAt                                                                                                                                                                                                        <  1:1057956/233‑1 (MQ=255)

                                        tcGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGGCTATA‑AGGCGACCTATACCGCCTAAACCAAAGGCAGGGGACTTACTCCAAAGcctta                                                                                                                                                                                                       >  2:1261030/1‑235 (MQ=255)

                                            cAATTAATATGTAAAAGGACCTTTACCCCTCCGGAAACCCTATCGAGGTGAGGGTAGAACTGAGAGAAGAAAAGGACAAA‑CCTTTAAAGGAAAAAAAACAGCAACTGAATACCGCAGACAGCATCGAC‑AAGGTAAAACCAGGAGTCACTACAGACTGAAAAGAACCCGAAGA‑TGGCGTCTATA‑AGGCGACCAATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATg                                                                                                                                                                                                   <  1:696069/239‑1 (MQ=255)

                                                 aaGATTGAAAAGGCCCGTTATCCCTCCGGAAATCCTACAGAGGTGAGGGTAGAACTGAGAGAGGAAAATGACAAA‑CCTGTTAAGGAACAAAACCAGCAACTGAATAACGCAGTCAGCACCGAC‑AGCGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCaaaa                                                                                                                                                                                              <  1:1047964/239‑1 (MQ=255)

                                                    aTTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAGA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGAAAAGAAACCGCAGA‑TGGCGACTATA‑AGGCGACCTATACCGCCTATACAAAAAGCAAGGGACATACtcgaaaaatattaaatcaaaaatg                                                                                                                                                                                           >  2:534102/1‑216 (MQ=255)

                                                    aTTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCCACTGATTACCGCAGTCAGCATCGCC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGCAACCGCAGA‑TGGCGTCTCTA‑AGGCGACCTATACCGCCTATACCACAGGCAGTGGACTTACTGCGAAGCAATTAATGCAAAACTg                                                                                                                                                                                           >  1:793301/1‑239 (MQ=255)

                                                      tGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGAAGTCAGCATCGAC‑ACCGTGAAACCAGGAGTCACTACAGACTGGAAACAAACCGAAGG‑TGGCGGCCATA‑AGACGACCTAAACCGCCTATACCAAAGGCAGTGGACTTTCTGGGAAGCTATTAATGCAAAACTTGa                                                                                                                                                                                         >  2:1085756/1‑239 (MQ=255)

                                                        aTAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAACCCGCAGA‑TGGCGCCCATA‑AGGCGACCTATACCGCCCATACCAAAGGCAGGGGACTTACTGCGGAGCTCTTAATGAAAAAATGGAAt                                                                                                                                                                                       >  2:929817/1‑239 (MQ=255)

                                                        aTAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGC‑TGGCGCCTATA‑AGGCGCCCTAAACCGCCTATACCAAAAGAAGAGGACTTAATGTGCACCCATTAAGGCAAAAATGGaaa                                                                                                                                                                                       >  2:606167/1‑238 (MQ=255)

                                                         tAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCATA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCACAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATg                                                                                                                                                                                      >  1:871647/1‑239 (MQ=255)

                                                         aagggccctttATCTCTCGGGCAATCCTTCAGGGGTGACGGTAAAACTGAGAGATGAAAAGGACAAC‑CCTGTTAAGGAACAAAACAGGCAACTGAAAAACGCAGTCAGCATCGCC‑AACTGGAACCCGGGAGTCACTACAGCCTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATg                                                                                                                                                                                      <  1:628139/230‑1 (MQ=255)

                                                           aGGACCGTTACCCCTCCGGCAACCCTACGGAGGGGAGGGTAGAACTGAGAGATGAAAATGAAAAA‑CCTGTTAAGGAAAAAAAACAGCAACGGAATAACGCAGTCAGCATCGAC‑AACGTAAAACCAGGAGTCACTAAAGACTGGAAAGAACCCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGaa                                                                                                                                                                                    <  1:1261030/239‑1 (MQ=255)

                                                              aCCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTCCGAAGCTATTAATGCAAAACTGGAATGAAGAt                                                                                                                                                                                 >  1:373058/1‑239 (MQ=255)

                                                               ccGTTATCTCCCCGGAAACCCGAGCGAGGGGAAGGTAAAATGGAGAAAGGAAAATGAAAAC‑CCTGTTAAAGAACAAAAACAGAAACTGAAAAACGCAGTCAGCATCGAC‑AACGTAAACCCAGGATTCACTAAAGCCGGGAAAAAAACCGCAGA‑TGGGGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGAt                                                                                                                                                                                 <  1:117387/238‑1 (MQ=255)

                                                                  ttATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGCCTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGCCAGTGGACTTACTGCGAAGCAATTAATCTAAAACTGGAACTAACATTTGc                                                                                                                                                                             >  1:225620/1‑239 (MQ=255)

                                                                  ttATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGc                                                                                                                                                                             >  1:1100695/1‑239 (MQ=255)

                                                                  ttATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGGGGACTTACTGCGAAGCCATTAATGCAAAACTGGAATGAAGATTTGc                                                                                                                                                                             >  1:812070/1‑239 (MQ=255)

                                                                  ttATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACAAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGCCTTACTGCGAAGCTATTAATg                                                                                                                                                                                                   >  1:257798/1‑217 (MQ=255)

                                                                   tttctcccCGGCACCCCTATCGA‑GAGAAGGGAAAAACGGGAGAATAAAAAGACAAAGCCGGTAAAGAAAAAAAAACACCACCAAAAAAACTAAATCAGCACCTAC‑AATGTAAAACAGGGAGCCCTCACCAACTGAAAAAAAACCGAAAT‑GGGCGTCAATA‑AGGCGCCCTAAACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCa                                                                                                                                                                            <  2:649997/237‑1 (MQ=255)

                                                                   ttaccctcCGGAAATCCTAACGGGGTAAAGGTAGAAATGAGAGATAAAAATGACAAA‑CCTGTAAAGAAACAAAAACAGCAACTAAAAAACGAAGTAAGCATCGAC‑AACTTGAAACCAGGATTCACTAAAGACTGGAAAAAAACCGCAGA‑GGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCa                                                                                                                                                                            <  1:606167/234‑1 (MQ=255)

                                                                       tctcCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATAcc                                                                                                                                                                        >  1:1310892/1‑239 (MQ=255)

                                                                        ctcCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTAACCCGCCTATACCAAAGGCAGTGGACTTACTACGAAGCTATTAATGCAAAACAGGAATAAAAATTTgacaacc                                                                                                                                                                        >  2:631608/1‑232 (MQ=255)

                                                                        cccggacaatcttgcgggggggACGGTAAAACTGAAGAAAAAAAAGGACAAA‑CCTGTTAAGAAAAAAAAACAGAAACGAAATAACACATTCAGCATCGAC‑AAGGGGAAACCAGGAGTACCTACAGACGGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCg                                                                                                                                                                       <  2:657419/218‑1 (MQ=255)

                                                                                 tCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTa                                                                                                                                                              >  1:193657/1‑239 (MQ=255)

                                                                                 tCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTa                                                                                                                                                              >  1:351536/1‑239 (MQ=255)

                                                                                      tCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAACCTGGAATGAAGATTTGCATACCGCTGGATTTatca                                                                                                                                                           >  1:1346102/1‑237 (MQ=255)

                                                                                           gTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCAACg                                                                                                                                                     >  1:819981/1‑238 (MQ=255)

                                                                                              aCGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCAATTAATGCAAAACTGGAAGAAAAATTTGCATACCGCTGGAttttacaacgaaccaa                                                                                                                                                  >  2:67600/1‑225 (MQ=255)

                                                                                                            agaTGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATGAACGCCAACACCCAGtaaagga                                                                                                                                   >  2:575973/1‑233 (MQ=255)

                                                                                                            agaTGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGAATGGAAAGAAACGGCAGA‑TGGCGTCTATA‑AGGCGCCCAATACCGCCTATACCAAAGGAAGTGGACTTACTGCGAGGCTATTAATGCAAAACTGGATAGAAGATTTGCATCACGCAGGCTTTTACAACAACCCAAACCCGCAgaaacgga                                                                                                                                   >  2:506968/1‑232 (MQ=255)

                                                                                                                        aaaaatcggttagagAAAAAAAACAGAAACTGAAAAACGAAGTCAGCACCGAC‑AAGGTGAAACCAGGAGTCACTACAGCCGGAAAAGAAACCGTAGA‑TGGCGTCTATA‑GGGCACCCTAAACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGa                                                                                                                          <  1:1179149/223‑1 (MQ=255)

                                                                                                                           a‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATAACGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAAATGCAACaaaat                                                                                                                    >  2:221848/1‑235 (MQ=255)

                                                                                                                           a‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGACAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTAACCGAAAATTGCGACATAAt                                                                                                                    >  1:677568/1‑239 (MQ=255)

                                                                                                                                 ttAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCCACGAAAATTGCGACCTTATCTgac                                                                                                               >  1:939558/1‑237 (MQ=255)

                                                                                                                                 taaaGAAAAAAAAACACCACCGAAATAACCAAGCCAGCATCAAA‑AACGTGAAACCAGGACTCACGACAGCCGGAAAAAAACCCGAAGA‑TGTGTTGTATA‑AGGCAACCTAATCCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGcc                                                                                                               <  1:1083712/237‑1 (MQ=255)

                                                                                                                                  aaaGGAAAAAAAGCAGCAACCAAATACGGCATGCAGCACGGAC‑ACCGTGAACCCAGGAATCCTGACGAATCGGAAAGAACCCGCAGA‑TGGTCTCTATA‑AGGCGACCTATGCCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGcc                                                                                                               <  1:720395/237‑1 (MQ=255)

                                                                                                                                        aCAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATACACGCCAACCCGCAGCCAGCGAAAATTGCGACCTTATCTCCCAGCaata                                                                                                        >  1:1348460/1‑239 (MQ=255)

                                                                                                                                         cAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGCAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTTCCAGCaataa                                                                                                       >  1:112097/1‑239 (MQ=255)

                                                                                                                                             aaccgaaaCTAAATACCGCAGTCAG‑AACGACAAATGTCAACCGCGGAGTCCCAACAGAGTGGAAAAAACCCGCATG‑GGGCGTATAAA‑AGGCGACTTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGgt                                                                                                   <  2:1359790/233‑1 (MQ=255)

                                                                                                                                                aGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTAAGCGAAAATTGCGAAATTATCTGCCAGCAATAATGgtgt                                                                                                 >  1:5011/1‑238 (MQ=255)

                                                                                                                                                 gccaCTGTATAACGCAGTCAGCATCGAC‑AACGTAAAACCAGGAGTCACTCCAGACTGGAACGCAACCGCAGA‑TGGCGTCAATA‑AGGCGACCTATACCGCCTATACCAACGGCAGTGGACTTACTGTGAAGCTCTTAATGCAAACCTGGAGTAAGGATTTTCATACCGCCGGCGTTGTCATCGATGCCAAACACCAGTCAGCAAAAAATGCGACAATATACGCCAGccaaaaaagggtg                                                                                                >  2:864514/4‑226 (MQ=255)

                                                                                                                                                  cAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGCCATTATCTGCCAGCAATAATGGTGTGc                                                                                               >  1:39197/1‑238 (MQ=255)

                                                                                                                                                         aaaacccagcaaGCCTCGAA‑AACGTAAAACCAGGGGTCACTACAGCCTGGAAAGAACCCGCAGA‑TGGCATCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAAt                                                                                       <  1:575973/228‑1 (MQ=255)

                                                                                                                                                           cacccAGTCAACATCGAC‑AACGTAAAACCAGGAGTCACAACAGACTGGAAAGAAACCGCAGA‑TGGCTTTTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATga                                                                                     <  2:229247/235‑1 (MQ=255)

                                                                                                                                                           acctcaatcaacttcaaC‑AACGTGAAACCAGGAGTCACTACAGACTGAAAAAAACCCGAAGA‑TGGCGTTTATA‑GGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATga                                                                                     <  2:1310892/223‑1 (MQ=255)

                                                                                                                                                             cGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTCCGACATTATCTGCCAGCAATAATGGGGTGCTCGCCAATgaga                                                                                   >  1:1086809/1‑239 (MQ=255)

                                                                                                                                                                  tCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTTTGCTCGCCAATGGGAATgca                                                                              >  1:922651/1‑239 (MQ=255)

                                                                                                                                                                  tCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATgac                                                                              >  1:806005/1‑237 (MQ=255)

                                                                                                                                                                  tCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAAAAAAGGGATGCACGCCAAtaaaaaagaa                                                                              >  2:130786/1‑230 (MQ=255)

                                                                                                                                                                  tCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCCACGCCAACCCGCAGTAAGCGAAAATTGCTCAATTTTCTGCCAGCAATAAAGGTGGGCCCGCCAATGAGAATgcc                                                                              >  2:320657/1‑238 (MQ=255)

                                                                                                                                                                  tCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATAATCGATGCCAACCCGCAGTCAGCGTAAATTGCGAAATTATCTGCCGGCCAAAATGGTGTGCTCGCCAATGAGAATgc                                                                               >  1:726569/1‑238 (MQ=255)

                                                                                                                                                                  tCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCC‑‑‑ACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATCAGAATgcagca                                                                           >  1:366261/1‑239 (MQ=255)

                                                                                                                                                                  ccaaatgagacaaagttaaaaCCAGGAACCCCCAAAGACGGAAAAAAACCCGCGGA‑‑GGCGTTTATAGAGGGCCCCTAAACCGCCTAAACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCACCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATgc                                                                               <  2:1100695/221‑1 (MQ=255)

                                                                                                                                                                          gggaaaagggAAACCAGGAGCCACTACAGCTTGAAAAAAACCCGCAGG‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAaca                                                                       <  1:1263618/230‑1 (MQ=255)

                                                                                                                                                                               acggaaacccgggggTCACAAAAGACTGGAAAGAAACCGAAGA‑TGGCGTCAATA‑AGGCGCCCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGtc                                                                  <  1:130786/225‑1 (MQ=255)

                                                                                                                                                                                 gTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGCAATTATTTGCCAGCAATAAAGGTGGGTCCGCCAATGGGAAAGAAGAAAACACCgcacc                                                                >  2:1263618/1‑235 (MQ=255)

                                                                                                                                                                                          ggAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCGGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAGATTGCGACATTATCTGCCAGCAATAAAGGGGTGCTCGCCAAAGAGAATTCAGCAAACACCGGCTCGGGAAATgc                                                       >  2:485059/1‑238 (MQ=255)

                                                                                                                                                                                           gAGTCATTACAGCATGGAAAAAACCCGAAGA‑TGGCCTCTAAA‑AGGCAACCTAAACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGt                                                       <  1:8046/238‑1 (MQ=255)

                                                                                                                                                                                           gAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGGGACATTATCTGCCAGCAATAATGGTGTGCTAGCCAATGCGAATCCAGCAAACACCGCACCGGTCAATGTc                                                      >  1:197319/1‑239 (MQ=255)

                                                                                                                                                                                             tactcTAAAGCCTGAAAAAAAACCG‑AGAGTGGCTTATAAA‑AGGCACCTTATCCCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCg                                                     <  2:124013/234‑1 (MQ=255)

                                                                                                                                                                                                       aCTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGGGAAAATTGCGACATTATCTGCCAGCAATAATGGAGTGCTCGCCAATGAGAAGTCAGCAAACCCCCGCTCGGTCACTGTccccggtaaggga                                          >  2:42678/1‑227 (MQ=255)

                                                                                                                                                                                                       aCTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATgaagga                                          >  1:455619/1‑239 (MQ=255)

                                                                                                                                                                                                       aCTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGGGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTctgcttcttata                                           >  1:1084810/1‑227 (MQ=255)

                                                                                                                                                                                                            aaagaaaCCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCa                                      >  1:792339/1‑238 (MQ=255)

                                                                                                                                                                                                             aagaagCCGCAGT‑GGGCTTCTATA‑AGGCAACCTAGCCCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAAc                                    <  1:51939/239‑1 (MQ=255)

                                                                                                                                                                                                             aaaaaaCCGCAGG‑AGGCGTCAAAA‑AGGCGACAAAAACCGACTATACCAAAGGCAGGGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAAc                                    <  1:556464/236‑1 (MQ=255)

                                                                                                                                                                                                              aaaaaacctgagtgggcgtaaaaa‑aGGGGACCTAAGCCGCCTAAACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAAc                                    <  1:354940/216‑1 (MQ=255)

                                                                                                                                                                                                                aaaCCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCca                                 >  1:831558/1‑239 (MQ=255)

                                                                                                                                                                                                                           tGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGGCTCGGTCAATTTCGCTGATGAGGGAAGCAACCCAATCAATAATc                       >  2:869585/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATAcc                  >  1:372467/1‑238 (MQ=255)

                                                                                                                                                                                                                                    ta‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGtc                                                                  >  2:588431/1‑187 (MQ=255)

                                                                                                                                                                                                                                           gACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGAGTGCTAGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGCCGCTGATGAAGGCAACAACCCAACAATTGATCATACCGTCACGTTTg        >  1:1136901/1‑239 (MQ=255)

                                                                                                                                                                                                                                            gctcataCCGCCTAGACGAAAGGCAGTGGACTTAGTGCGAATGTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTg        <  1:863097/234‑1 (MQ=255)

                                                                                                                                                                                                                                            aCCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAAt                                                         >  1:583429/1‑189 (MQ=255)

                                                                                                                                                                                                                                            aCCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGCTGAAGGAAGCAACCCAACCAATAATAAAACCGCCCCGTTTGc       >  2:384344/1‑239 (MQ=255)

                                                                                                                                                                                                                                            aCCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCCATGATCATACCTCCAAGTTTGc       >  1:189539/1‑239 (MQ=255)

                                                                                                                                                                                                                                            aCCTATACCGACTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAAt                                                         <  2:583429/189‑1 (MQ=255)

                                                                                                                                                                                                                                             ccTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGCAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAACCACCGTCTCGGTCCATGTCCCTGATTAAGGAAGCAACCCAAACAATGAACAACCCTTCACGTTTGCg      >  2:608946/1‑239 (MQ=255)

                                                                                                                                                                                                                                             ccTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCCCGTTTGCg      >  1:1037164/1‑239 (MQ=255)

                                                                                                                                                                                                                                             ccTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGAAACCCAAACAATAATCAAACCGCCACGtttcgg      >  2:561961/1‑236 (MQ=255)

                                                                                                                                                                                                                                             ccTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGCCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCg      >  1:803747/1‑239 (MQ=255)

CGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAATGACAAA‑CCTGTTAAGGAACAAAAACAGCAACTGAATAACGCAGTCAGCATCGAC‑AACGTGAAACCAGGAGTCACTACAGACTGGAAAGAAACCGCAGA‑TGGCGTCTATA‑AGGCGACCTATACCGCCTATACCAAAGGCAGTGGACTTACTGCGAAGCTATTAATGCAAAACTGGAATGAAGATTTGCATACCGCTGGATTTATCATCGACGCCAACCCGCAGTCAGCGAAAATTGCGACATTATCTGCCAGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTAT  >  NC_000913/2046806‑2047281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`