Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,978,164 | T→A | 23.4% | Y245F (TAT→TTT) | lysA ← | diaminopimelate decarboxylase, PLP‑binding |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,978,164 | 0 | T | A | 23.4% | 95.7 / 6.0 | 53 | Y245F (TAT→TTT) | lysA | diaminopimelate decarboxylase, PLP‑binding |
Reads supporting (aligned to +/- strand): ref base T (26/14); new base A (2/11); total (28/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.13e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGAT > NC_000913/2977935‑2978402 | ggCCCGGGCTCTGATAGGGTGGTAACCCACGTACCTTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCCCAAAGT‑GGCGGCCCCCCATTTGTTTGACGCTCCGCCCCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGtt < 2:930691/238‑1 (MQ=255) tgtgtataaaaggcagggtggtAACTTCCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTGGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGtt < 1:675618/222‑1 (MQ=255) ttgttttgggtttAACCACCCTACATTGCCGGGCCCATCGATTCGTTGACCCCGGCATCAACCCGCA‑AAAGTGGGCGGCCCCCCATTTGTTGGCCGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAAc < 2:1060647/227‑1 (MQ=255) attattttgttgtAACCACCGTATATTCGCGGGCCCTCCAGTTCGTTGAACCCGGCCACCACCAGCCAAAGGG‑GGCGGCCCCCCCTTTGTTTGACGCTCCGCCACGGAGT‑AATTAATACGCCAGACGGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACGGGGTGGCCAAAATGGCGGGCTATTTGCTCACCCGCGCCATCCCACAGAC‑CATAATAATGTTCGGTATCAAc < 2:472045/228‑1 (MQ=255) ttattgtggggtAACCACCGGTCATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTc < 2:1208501/220‑1 (MQ=255) gtggtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGGTTTGCTCCCGCGCGGCATTCCACAGAC‑CCTAATAAAGTTCGGTATCAACCGCccct > 1:463565/1‑238 (MQ=255) tgtaacacaCCGTACATTGCGGGGCCCAGCAGTTCTTTGACCCCGCCCACACCCAACAAAAGGG‑GGCGTCTCCCATTTTGTTTGACGCCCCGCCCCTGATT‑AATTAATACGCAAGACTGTGCTACCAGGAAGGGCCCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCAAAAGGGCGGGCTATTTGCTCCCGCGCGGCATTCCACAGAC‑CAAAAAAATGTTGGGTACCAACCGCCTCtt < 2:1141459/230‑1 (MQ=255) gtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGGGATTTGCTCACACCCGGCATTCCCCAGAC‑ACAAATAAAGTTCGGGTTCAACCGctcgtcctt > 1:1208501/1‑231 (MQ=255) gtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAAGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAAAGTTCGGTATCAACCGCCTCTTCa > 1:1060647/1‑238 (MQ=255) aCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTTACGCACCGCACCCGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑GTCAATTT‑TCAGTTTCCACGGGTGGCCCAAAAAGGCGGCACTTTGCTCACCCGCGGCCTTCCACAGCA‑CATAATAAAGGTCgggttacaccaccccttaccccc > 2:750115/1‑218 (MQ=255) aCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCCCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTTCCAGGGTGGCCCAAATGGCGGGCGATTTGCTCTCGCGCGGCATTCCCCAGAC‑CCTAATAATGTTCGGGATCAACCGCCCCCTCAc > 1:234078/1‑236 (MQ=255) ccGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCCTTTGTTTGACGCTCCCCCCCCGAGA‑AATTAATACGCCCGACTGCGCTACCCGGAAGCGGCCCCG‑GTCCATTT‑CCCATTTTACAGGGGGGCCCAAAAAGGGGGCGGCTTTCTCTCCCGGCGCCTTTCACAAAA‑CAAAATAAAATTCTGTGTCAAACCCCTCTTctttaaaac > 2:675618/1‑230 (MQ=255) tGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑GTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCCCAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCCGTTGATAAGGAACAGAAAGCCCAccgcccccc > 1:186774/1‑236 (MQ=255) ccGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCCCCCCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCCG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCCAAAGGCGGGCGATTTGCTCACGCGCGGCGTTTCCCAGAC‑CATAATAATGTTCGGTGTCCACCCCCCCTCCACCCTCTTGTTAAGGAAAAAAAAACACACCGCCCCCCGaaaa > 2:1219285/1‑238 (MQ=255) tgACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAACCCACCGCCCCCAGAAATAGCCTGTAAATCCTGACCGAAATCGATGACCTGACGAAcc > 2:1208694/1‑239 (MQ=255) gCTCCGACCCTGAGT‑ATTTAATACGCCAGACTGCGCTACCAGGAGGCGACCCGG‑TTCAATTT‑CCAGTTCCACAGGGTGGCCCAAAGGGCGGGCGTTTTGCTCACGCGCGGCATCCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAg < 1:1219285/239‑1 (MQ=255) ccTCCGCACC‑GAGTAAATTAATCCGCAAGACTGCGCTCCAAGGAGGCGCCCCGG‑TTCAATTC‑CCAGTTCCACGGGGTGGCCAAAAGGGCGGGCAATTTGCTCACGCGCGGCATTCCACAGCC‑AATAAAAATGTCGGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAg < 1:1208694/238‑1 (MQ=255) ttgtttaaagggaaagtaatCCGCCGGACGGCGCTCCCAGGAAGCGCCCGGG‑TTCAATTT‑CCATTTGCACAGGGTGGCCAAAATGGGGGGCGTTTTGCCCACGCGCGGCATCCCACAGAC‑AAAAATAATGTCCGGTACCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAg < 2:8517/221‑1 (MQ=255) gccttggcgttatttacaccccAGACTGCGCGACGAGGAGGCGACCCTT‑TTCAATTT‑CCGATGTCACAGGGTGCCCCAATGTGGGGGCGTTTTGCTCACTCGCGCTATTCCCAAGCC‑CATAATAGTTTGCGGTACCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGCCCTGACGCACCATAGcacca < 2:337628/219‑1 (MQ=255) aGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCt > 1:381135/1‑239 (MQ=255) aGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATCCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGACAGCCCACCGCCCGCAGAAATAGCATGTAAATCCTGACCGAATTCGAGGACCTGACGCACCATAGcacctgaccctt > 1:1336188/1‑231 (MQ=255) t‑aattaatACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGTTAAGGAACAGAAAGCCCCCCCCCCCCAGAAAAAGCCTGTAAACCCTGACCGAATTCGGTGACCtcaacacccaaaacccccaaca > 2:1120278/1‑213 (MQ=255) agccGCAAGGCTGCGCAACCAGGAAGCCACCCGG‑TTAAATTC‑CCAGTTTCCCGGGGGGGCCAAAATGGCGGGCAATTTGCTCACGCGCGGAATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGa < 1:1008958/236‑1 (MQ=255) aCGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CCTAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTAGATGAACTGACGCCCCCTACCACCACCCACCCGTTCCaacga > 2:1008958/1‑235 (MQ=255) caagctgccttACCAGGAAGGCACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCAAAAGGGCGGGCAATTTGCCCACGCGCGGCATTCCACAGAC‑AATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCa < 1:1179127/229‑1 (MQ=255) aCTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACAccctgtcccttttacacat > 1:8517/1‑221 (MQ=255) cgcTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCCGTTCCAGATGGGCATAATCa > 1:209642/1‑238 (MQ=255) cgcTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAAAAGCCTGTAAATCCTCACCGAATTCGATGACCCGACCCACCACAACCACACCACCCTGTTCCCGCTGGGCATAAtaa > 2:938049/1‑236 (MQ=255) gcTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACCCCTGTTCCAAATGGGCATAATCaa > 1:1270711/1‑238 (MQ=255) ccgggagggcccccGT‑TTCAATTT‑CGAGTTTCCAGGGGGGGCCCAAAGGGGGGGGTGTTTGCGCCCGGGCGGATTTCAAAAGAC‑AAAAATAATGTGCGGTACCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCa < 2:755384/228‑1 (MQ=255) ccAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACCCGCGGCATTCCACCGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAAAAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGCCCGAATTCGCTGACATGACCGCCCAAAGCACCACCCACCCGTTCCAAGTGGGCCAAAACAACCCCa > 2:1307120/1‑239 (MQ=255) aaGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTACATCCTGACCGAATTCGATGACCTTACGCACCATAGCACCACACCCCCGTCCCAACTGGGCTTAATCAACGCCAAAAcc > 1:128334/1‑239 (MQ=255) gcgcccGG‑TTTTATTTCCCATTTTCAGGGGGGGCCCAAAATGGCGGGGAATTTCCCCCCGCGCGGCATCCCACAGAC‑CAAAATAATGTCCGGTACCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa < 2:381135/235‑1 (MQ=255) gcccccGG‑TTAATTTT‑CCATTTCCCAAGGGGGCCCCAAAGGGGGGGGTTTTTGCCCACCCGCGGCATCCCACAACC‑AAAAAAAAGGTGCGGCATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa < 2:1270711/234‑1 (MQ=255) agACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTTCACAGAC‑CATAATAATGTTCGGTATCCACCGCCTCTTTACCCTGTTGATAGGGAACAGAAAGCCCCCCGCCCGCAGAAATAGCCAGTAAATCCTGACCGACTTCGCTGCCCTTACCCACCATTGCACCACCCACCTTTTCCAACTGGGGTTATAAAACGCCCGAACCacc > 2:1036991/2‑237 (MQ=255) gACCCGGTTTCAATTT‑CCAGTTTAACAGGGTGGCCAAAATGGCGGGCGATTTGCTCACGCGGGGCATTCC‑CAGGCACATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa < 1:938049/237‑1 (MQ=255) aCCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCCTAGCACCCCACACCTGTTCCAGATGGGCATAAt > 1:16505/1‑221 (MQ=255) cGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCCCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCCTAGCACCCCACACCGGTTCCAGCTGGGCATAATAAACGCCAGAACCAAAGTGCa > 1:552063/1‑239 (MQ=255) ttcccatgtccacaGGGTGGCCAAAAGTGCTGGCGATTTTCTCACGCCACGCATCCCACAGAC‑ACAAATGATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATg < 1:672938/229‑1 (MQ=255) gCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCacacac > 1:272719/1‑171 (MQ=255) cACGCGCGGCATGCCACAGCC‑CAAAATAATGTTCGGTACCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgcg < 1:305909/239‑1 (MQ=255) cACGCGCGGCATGCCAAAGAC‑CATAATAAGTTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgcg < 1:997651/239‑1 (MQ=255) ctaccACAGAC‑ATAAAGAATGTTCGGTACAAACCGCCTCTTCACCCTGTTGAAAAGGAACAGAAAGCCCCCCGCCCGAAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt < 2:683485/236‑1 (MQ=255) catCCACAGAC‑CATAAAAATGTCCGGTAACAACCGCCTCTTCACCCTGTTGAAAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt < 1:113186/237‑1 (MQ=255) agaaaagggccaataaaaATGTCCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt < 1:470486/227‑1 (MQ=255) cccaGAC‑AAAAATATTGTGCGGTATCACCCCCCTCTTCCCCCTTTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt < 2:16508/237‑1 (MQ=255) ccaaGAC‑AAAAATAATGTTCGTTAACAACCGCCTTTTCACCCTGGTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt < 1:1052968/236‑1 (MQ=255) agacagc‑cATAATAATGTTCGGTACCACCCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt < 1:912065/233‑1 (MQ=255) acaaGAC‑AAAAATAATTTTCGGTACCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt < 1:750115/236‑1 (MQ=255) aC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCACTCTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGGGCGGCGGGAACATCGGGGTAcc > 1:1354119/1‑239 (MQ=255) aC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGAACTAGTATCACGTCCAATGCGCCGGCCaaggccgcgtacc > 1:442342/1‑227 (MQ=255) aC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACCACAGAACCAATGTGCATGGGAATGCCGACCAGCTTCAGATGATGACGTTGTATCACGTCCAGAGCGGCgggacaaaagggggacc > 2:499725/1‑225 (MQ=255) aC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGGTCCAGATGGGCATAATCAACGCCAGAACCAATTGGCATGTGAATCCCGACCAGCTGCAGCGGATTACGTTGTATAACGCCCAGGGAGGGGGGCGGagagggggagc > 2:96286/1‑229 (MQ=255) cATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCAAACACCTGTTCCCATTGGGCATAATCAACGCACGAACCAATTTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCCGATCGGGGTACCaa > 2:1060460/1‑238 (MQ=255) taataaTGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCGGTGCGGCGGGAAGATCGGTGTACCaccg > 1:683485/1‑236 (MQ=255) | TGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGT‑GGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGT‑AATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGG‑TTCAATTT‑CCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAC‑CATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGAT > NC_000913/2977935‑2978402 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |