Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	249,538	T→G	26.4%	pseudogene (533/1713 nt)	lfhA ←	pseudogene, flagellar system protein, promoterless fragment, flagellar biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	249,538	0	T	G	26.4%	74.8 / 3.8	77	pseudogene (533/1713 nt)	lfhA	pseudogene, flagellar system protein, promoterless fragment, flagellar biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base G (19/7);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 2.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGTT  >  NC_000913/249308‑249773                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 gCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGTTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCAt                                                                                                                                                                                                                                         >  1:435091/1‑238 (MQ=255)  gctgtttcccttgtcTTTGCCCCCCAGCGCCACCTTTTTGTAACCGGGGCTTAAACTGTTGGGCGCGATCAGGGGAATGGTTTCCCAACTGCCCTGCTGCTCGCTGGTTT‑G‑GGTGGTGGTGCGGGTCAGCGTTTCGGGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCACCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCAtt                                                                                                                                                                                                                                        <  2:629212/224‑1 (MQ=255)      ttACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGG‑G‑GCATTCCC‑gg                                                                                                                                                                                                                                  >  1:474013/1‑239 (MQ=255)              tgCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCCCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTGAAACCAAG‑CAGGGCGC‑TGAGCAGA‑AAAAA‑CGGCC‑GGGGG‑G‑GGACTCCC‑gcggcaacc                                                                                                                                                                                                                           >  2:107618/1‑230 (MQ=255)               gCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑CAAAC‑AGGCA‑GGGGG‑G‑GCATTCCC‑GGT‑acccccc                                                                                                                                                                                                                         >  1:1190345/1‑237 (MQ=255)               gCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGG‑accaccc                                                                                                                                                                                                                         >  1:755586/1‑237 (MQ=255)               gCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCCGTCCC‑GGT‑CACACCg                                                                                                                                                                                                                         >  1:152174/1‑238 (MQ=255)               gCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCACTGGTTT‑C‑GGTGATGGTGCGGGTCAGGGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGG‑AAAAA‑CAGCA‑GGCGC‑G‑GCATTCCC‑GGG‑ACCCCCg                                                                                                                                                                                                                         >  1:1228219/1‑238 (MQ=255)                   tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTTCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAAAAGC‑AAAAA‑CGGCA‑AGGGA‑G‑GCGTTTCC‑CGT‑ACCACCGCCAg                                                                                                                                                                                                                     >  1:74636/1‑238 (MQ=255)                   tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGGCC‑TGACCAGC‑AAAAA‑CGGCAGGGTGG‑G‑GCGTTCCC‑ggaccccccccccc                                                                                                                                                                                                                      >  2:1150788/1‑227 (MQ=255)                   tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AGAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCCCCGcccg                                                                                                                                                                                                                     >  1:1011975/1‑236 (MQ=255)                   tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGCCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGc                                                                                                                                                                                                                    >  1:234142/1‑239 (MQ=255)                   tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑GTTGCTCATCCGCCAGCCGGTAAAACCCAG‑CAGGGCGC‑TGAACCGC‑AAAAA‑AGGAC‑GGGGC‑G‑GCATTTCC‑GTG‑ACCACCCCCac                                                                                                                                                                                                                     >  1:193031/1‑237 (MQ=255)                     tCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCCGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑CAAAA‑CGGCA‑GGGGG‑G‑GCCTTTCC‑GGG‑ACCCCCGCCaccc                                                                                                                                                                                                                   >  1:614381/1‑235 (MQ=255)                        accaGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGT‑ACCACCCCCCGCACGa                                                                                                                                                                                                                >  1:1373424/1‑238 (MQ=255)                         ccccccccccctgTTTGTAACCGAGGGTTTAAACGATTGGCTTCGTCGGGGGGATTGTTTCCCCGATGGCCCGGTGCTCGGTTGTTT‑CGGGGGATGGGGCGGTTCGGGGTTCGGGGGTTTTTCTCTTCCCCCCCGGCGCCCAGCGGCTG‑TTTGCCCACCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCG‑GGTGC‑G‑GCATTCCC‑GGT‑CCCACCGCCAGCACGaa                                                                                                                                                                                                               <  2:74874/227‑1 (MQ=255)                             cgcCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCC‑GGCGC‑G‑GCATTCCC‑GGG‑ACCACCGCCAGCACCAACATa                                                                                                                                                                                                           >  1:394942/1‑238 (MQ=255)                               ccaccaGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCTC‑CGG‑ACACCCGCCAGACCAAACACAAt                                                                                                                                                                                                         >  1:1314589/1‑238 (MQ=255)                               ccaccaGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGG‑ACCACACCCAGCACGAACATaaa                                                                                                                                                                                                         >  1:853514/1‑237 (MQ=255)                               ccaccaGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCCGTAAAACCAAG‑CCGGGCCC‑TGAACAGC‑AAAAA‑CGGCC‑GGTGC‑G‑GCAATCCC‑GGT‑ACCACCACCACCACCAACCTAAt                                                                                                                                                                                                         >  1:227050/1‑238 (MQ=255)                                      tttGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGTGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCCTCCGCCAGCCGGTACAACCCAG‑CACGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GG‑GC‑GCGGAATCCC‑CGT‑ACCACcccccgacccaaccaaaaccccgagg                                                                                                                                                                                                 >  2:215854/1‑217 (MQ=255)                                      tttGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTgg                                                                                                                                                                                                 >  1:281267/1‑239 (MQ=255)                                                aGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACTGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGGCCGGACATAATCCCGGGCGCGgtgtgagg                                                                                                                                                                                       >  1:598766/1‑235 (MQ=255)                                                aGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGA‑G‑GCCTTTCC‑CGT‑ACCAACGCCAGACAGAACCTAATACCGGTGgggggggtag                                                                                                                                                                                        >  1:91351/1‑232 (MQ=255)                                                aGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑AAGGGCGC‑TGAAAAGC‑AAAAA‑CGGCC‑GGGGC‑G‑GGATTTCC‑CGG‑ACCCCACCCCGCACCAACACAAGACCgcgggcgggggaaag                                                                                                                                                                                       >  2:824480/1‑232 (MQ=255)                                                      aaatggaggggctgaatcgggggAATGGTTTCCCAACTGACCTGCGTCTCG‑TGGTTTCC‑GGTGGTGGGGCGGGTCAGCGTTCCGAGGCTTTTCTCTTCCGCCGCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGa                                                                                                                                                                                 <  1:788703/218‑1 (MQ=255)                                                            aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCCGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACAACCGCCAGGACGCACATAATCCCGGTGGCGGGTGAGAGCACCCACGGGCt                                                                                                                                                                           >  1:1060534/1‑239 (MQ=255)                                                            aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TTAACAGC‑AAAAA‑CGGCA‑AGGGCGG‑GCATTCCC‑GG‑‑ACCACCCACCACACGAACATAAACCCGGGTGCgggggtgaagacccacggggg                                                                                                                                                                           >  2:1345501/1‑220 (MQ=255)                                                            aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑AAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GGATTTCC‑GGT‑ACGCACGCCAGCACGGAATAAAACACCGTGGCGGGTGAGAGCAccacaggg                                                                                                                                                                             >  1:200541/1‑231 (MQ=255)                                                            aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑CTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑CGT‑ACCACCGCCACCCCGAACATAATCCCCGTGGCGGTGTAGAGCACCCACGGGCt                                                                                                                                                                           >  1:950329/1‑239 (MQ=255)                                                                 cTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CCGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCGTTCCC‑GGT‑CCCACCGCCAGCAACAAAAAAATCCCgggggggggggacggccaccacgggccggcc                                                                                                                                                                       >  2:730804/1‑215 (MQ=255)                                                                    gATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCACG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTGCC‑GGT‑ACCCCCGCCACCACGAACACAATCCACGTGGCGGTGTAGAGCACCGACGGGCTTGCcaaca                                                                                                                                                                   >  1:39885/1‑239 (MQ=255)                                                                    gATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑AACACCGCCAGCACGAACATAATCCCGGGGGCGGGGTAGAGCACCGACGGGCTTGCcagc                                                                                                                                                                    >  1:1131021/1‑238 (MQ=255)                                                                     aTCAGGGGAATGGTTGCCCACCTGCCCGGCTGCTCGCTGGTTC‑G‑GGTGAGGGGGCGGTTCAGCTTTTCGGGGCTTTTCTCTCCCGCCCCCGCCGCAGGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCcagca                                                                                                                                                                   <  1:911307/238‑1 (MQ=255)                                                                      tCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGGGGCGGTGTaga                                                                                                                                                                                        >  1:714209/1‑216 (MQ=255)                                                                              agggtcaccaatcTGCCCGGCTGCTCGTTGGTGT‑C‑GGTGTTGGTGGGGGTCAGCTTTCCGAGTCTTTTCTCTCCCCCCTCCCCCCCCAGCGGCTT‑TTTGCTCACCCGCCAGCCGGTAAACCCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACg                                                                                                                                                         <  1:730804/227‑1 (MQ=255)                                                                                    tCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTTCC‑GGT‑ACCCCCGCCCGCACGAACATAATCCCGGTGGCGGGGTAAGGCACCGACGGGCTTGCCAGCAGCTGGGTACGCACGt                                                                                                                                                    >  1:1032700/1‑238 (MQ=255)                                                                                    tCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGGGGCGGGGTAGAGCACCGACCGGCTTGCCAGCAGCCGGTTACCCCCGt                                                                                                                                                    >  1:1021769/1‑238 (MQ=255)                                                                                    ccccACCTGCCCTGCTGCTCGCTGTTTG‑C‑GGTGAGGGTCGGGGTCAGCGTTTCGAGGTTTTTCTCTTCCGCCTCCGCCGCCGGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTc                                                                                                                                                   <  1:579205/238‑1 (MQ=255)                                                                                          cTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGG‑ACCACCGCCAGCCCGAAAATAAAACCCGGGGCGGGGGAGAGGACCGCCGGGGTTGCCCACAGGCGGGTAAGCACGGCaaaggg                                                                                                                                             >  2:1363074/1‑234 (MQ=255)                                                                                           tGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCCCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCCTTCCC‑GCT‑CACACCGCCCGCACGGACACAAACCCCGGGGCGGGGGAAAGACCCCACCGGCCTGCCCGCAACCGGTGTCGCAcccccaggggg                                                                                                                                            >  2:1358756/1‑229 (MQ=255)                                                                                                        gCTGGTAT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGGTC‑G‑GCCTTCCC‑GGT‑ACCCCCGCCAACCCGAACACAAACCCGGTGGGGGTGTGGAGCACCCACCGGCTTTCCAGCAGCCGGGTACGCACGCCC‑TGGGCGGTCACGCCCTTGt                                                                                                                              >  2:1318950/1‑239 (MQ=255)                                                                                                                        gggggggggTCCGGGTTTCGAGGTTTTTCTCTCCCCCCTCCGCCGCCAGGGGCTT‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGt                                                                                                                 <  1:1075235/232‑1 (MQ=255)                                                                                                                             cGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCCTTCCC‑GGT‑ACCCCCGCCACCCCGAACATAATCCCGGGGGCGGGGTAGGGGCCCCACCGGCTTGCCCCCACCTGGGTACGGCCGCCAGTGGGGGATTTCCCCG‑TGTCGCCGAccgggggggggga                                                                                                           >  2:1124541/1‑227 (MQ=255)                                                                                                                               ggTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGGGTAGGGGACCGACGGGCTTGCCAGCAGCTGGTTACCCACGTCA‑TGGGCGGAATCCCCGTTGTGGCTGACGCGGGGGACGGTaa                                                                                                         >  1:1266286/1‑239 (MQ=255)                                                                                                                                      gTTGCGGGGCTTTTCTCCGCCGCCTCCGCCGCCAGCGGCTT‑TTGCCCCATCCGCCACGCGGTAAACCCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑CCCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATcgc                                                                                                     <  1:107618/236‑1 (MQ=255)                                                                                                                                               gttttCTCCTCCGCCCTCTCCGGCACGGGCTGTTTTTGTCTTCCCCCCGGCTGGAAAATCACGCCAGGGCGT‑GGAACAGC‑AAACA‑GGCCG‑GGTG‑‑G‑GCCTTCCCGGGT‑ACCACCGCCAGCACGAACATAAGCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTg                                                                                           <  2:323308/238‑1 (MQ=255)                                                                                                                                                ttttCTCTTCCCTCTCCGCCGCCAGCGGATG‑TGTGCGCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑GGAACAGC‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGga                                                                                         <  1:1044417/238‑1 (MQ=255)                                                                                                                                                ttttCCCTCCCGCCCCCGGCGCCCGGGGTTG‑TTGACATACCCCCCAGCCGGAAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAC‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑CCCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGgag                                                                                        <  1:1174470/239‑1 (MQ=255)                                                                                                                                                  tcctctCCCCCCTCCGCCCCAGGCGGTTG‑TTTCCACCACCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGagag                                                                                      <  1:824480/237‑1 (MQ=255)                                                                                                                                                     tccccccccccccccgccTGCGGTTT‑TTTCCCCCCCCCCCCGCCGGTAAAACCAGG‑CGGGGCGC‑TAAACGGA‑AAAAC‑GGGGA‑GGTGG‑G‑GCATCCCC‑GGT‑CCCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGagcag                                                                                   <  2:474013/226‑1 (MQ=255)                                                                                                                                                         ccgcctccgccGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTa                                                                                                                                                                                          <  1:1299122/133‑1 (MQ=255)                                                                                                                                                         ccgcctccgccGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTa                                                                                                                                                                                          >  2:1299122/1‑133 (MQ=255)                                                                                                                                                                             ttgttgcatcacccccaaccgggTAAACCAAAG‑CGGGGCGC‑TAAACAGA‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGccacc                                                             <  1:1193238/217‑1 (MQ=255)                                                                                                                                                                               tttcgcccATCCGCCCGGCGGTAAAACCAGG‑CAGGGCGT‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑CCCCCCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAgg                                                          <  1:791305/232‑1 (MQ=255)                                                                                                                                                                                 tggCTCACGCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAAAGGA‑AAAAA‑CGGCA‑GGTGG‑C‑GCATTCCC‑GGT‑GCCCGCGCCAGCACGAACATAATCCCGGGGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGcc                                                        <  1:15525/237‑1 (MQ=255)                                                                                                                                                                                   tccccccgcccgggccgTAAAACC‑AG‑GGGGGCGC‑GACACAGCAAAAAA‑CGGCG‑GGGGC‑G‑GGTTTCCC‑GTA‑CCCACCGCCAGCACGAAAATAAGCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGt                                                      <  2:950329/223‑1 (MQ=255)                                                                                                                                                                                   gCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCCGCGCCACCAGGccc                                                       >  1:330389/1‑237 (MQ=255)                                                                                                                                                                                        cccGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑AGAAAAGA‑AAAAA‑CGGAG‑GGTGC‑G‑GCTTTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCg                                                 <  1:1150788/238‑1 (MQ=255)                                                                                                                                                                                          cgcgcaccggggaaaCCCAG‑GCGGGCGCTTGAACGAC‑AAAAG‑CCGCG‑GGTGC‑G‑GCTTCCCC‑GGC‑CCCCCCGCCGGCACGAACATAATCCCGGTGGGGGTGTAGGGCACGGACGGGCTTGCCAGCAGCGGGTTTCGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGa                                                <  2:545634/227‑1 (MQ=255)                                                                                                                                                                                           gaccgccgtaaaaaCCAAT‑GGGGTCGG‑TGAACAGA‑AACAG‑CGGCA‑GGTGC‑G‑GCCTTCCC‑GGC‑ACCACCGCCAGCACCAACATAATCCCGGTGGCGGGGTAGAGCACCGACGGGGTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATg                                              <  2:91351/229‑1 (MQ=255)                                                                                                                                                                                             ccgCCGGTAACCCCCAG‑CGGGGCCA‑TGAAACAC‑AAAAC‑CGGGG‑GGGGG‑G‑GCTTTCCG‑GGC‑CCCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGt                                            <  2:1266286/237‑1 (MQ=255)                                                                                                                                                                                               gccgtaaaaaCCAGC‑CGGGGCCC‑TG‑ACAGA‑AAAAACCGGCA‑GGGGC‑G‑GCACTCCG‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGGCGGGCTTGCCAGCAGGTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtca                                          <  2:1032700/233‑1 (MQ=255)                                                                                                                                                                                                cccggtctaccctggcggggCCC‑TG‑ACAGC‑AAAAATGGGCC‑GGTGC‑G‑G‑ATGACC‑GGTGCCCCCCGCCAGCGCCAACATAACTCCGGTGGCGGTGTAGAACTCCGGCGGGCTTGCCAGCAGCTTGTTACGCACGTTG‑TGGGCGATTTCGCGGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtca                                          <  2:634469/222‑1 (MQ=255)                                                                                                                                                                                                ccGGTAAATCCGAG‑CGGGGCGC‑TGAAACGA‑AAACG‑CGGCG‑GGTGC‑G‑GCTGTCCC‑GGT‑ACCACCGCCCGCCCGAACATAAGCCCGGGGGGCGTGGAGAGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtcat                                         <  2:302256/239‑1 (MQ=255)                                                                                                                                                                                                 cGGTAAACCCAAG‑CAGGGCGC‑TAACAAGA‑AAAAC‑CGGGA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCCCCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtcat                                         <  1:1085502/238‑1 (MQ=255)                                                                                                                                                                                                 cGGTAAAACCAGA‑GAGGCCGC‑GAAACAGA‑AAAAA‑CGGCA‑GGGGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtcat                                         <  1:1318950/238‑1 (MQ=255)                                                                                                                                                                                                 cGGAAAACCCAAG‑CGGGGGGA‑GGAACAAA‑AAAAC‑GGGCG‑GGGGC‑G‑GCTTTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtcat                                         <  2:10916/238‑1 (MQ=255)                                                                                                                                                                                                   gTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCCCGGTGGAGAGCAGCAGGGAAGGGGTCTGCGCCCCCAGGCCGTCGCCGCTGGgcatca                                       >  1:10916/1‑238 (MQ=255)                                                                                                                                                                                                       acccAAG‑CGGGGCGC‑TAAACAGC‑AAAAG‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCTCCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGcaca                                  <  1:182047/237‑1 (MQ=255)                                                                                                                                                                                                          cagg‑CAGGGCGC‑GGAACAGC‑AAAAC‑GGGCG‑GGGGG‑G‑GCTTCCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATAc                               <  1:665595/236‑1 (MQ=255)                                                                                                                                                                                                                    cgc‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATcgc                                                                                                     <  1:233452/160‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaa‑CGGAG‑GGTG‑‑G‑GCGTTTCC‑GGTCACCCCCGCCAGCACGAACAGAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCgg            <  2:1079991/238‑1 (MQ=255)                                                                                                                                                                                                                                     a‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGa                                                                                                            >  1:1103258/1‑138 (MQ=255)                                                                                                                                                                                                                                     a‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTc       >  1:260491/1‑239 (MQ=255)                                                                                                                                                                                                                                           a‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCCCCTACTGCTGGGAGGCAGCAACGGCGCTCAgggt  >  1:1079991/1‑237 (MQ=255)                                                                                                                                                                                                                                             ggTGC‑C‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGGTGCGGGGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGtt  <  1:413812/238‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 GCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTT‑C‑GGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTG‑TTTGCTCATCCGCCAGCCGGTAAAACCAAG‑CAGGGCGC‑TGAACAGC‑AAAAA‑CGGCA‑GGTGC‑G‑GCATTCCC‑GGT‑ACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCA‑TGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGTT  >  NC_000913/249308‑249773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑