Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,175,798	T→G	20.5%	S69S (TCA→TCC)	yqiA ←	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,798	0	T	G	20.5%	168.7 / 2.1	94	S69S (TCA→TCC)	yqiA	acyl CoA esterase
Reads supporting (aligned to +/- strand):  ref base T (42/25);  new base G (3/21);  total (46/48)
Fisher's exact test for biased strand distribution p-value = 2.82e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAA  >  NC_000913/3175567‑3176036                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   ctccGTCTGTTGCAGCAGCCAGATCAAATCCGGCGATTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AAc                                                                                                                                                                                                                                           <  1:690249/237‑1 (MQ=255)     gTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACCTATTGCTGCCCGGTATCAGGGTTCTCGTTCTGTCCTAGATAGCCCGTCAGCAGTTCTAACGGGCGCACCGCCGGGTTT‑TCCACCAC‑TGCGGGACGACAAAAAAATTCGG‑ACAACCCAGTGACGTATAAACCCCCC‑A‑GTGAC‑G‑AAcagca                                                                                                                                                                                                                                       >  2:294183/1‑235 (MQ=255)     gTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGGGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGACCCGCCGGGTTT‑ACCCCCAC‑TGCGGGCAGCCTAAAACCTTTTG‑ACAACCAGGTGCCGAAAATCCCCCCC‑A‑GTgcccaccccac                                                                                                                                                                                                                                         >  2:748610/1‑229 (MQ=255)                                ggCGTTTCCAGCGGGTCAATCTGCATGACTTAAAGATCGTAAATTGGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCGGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTc                                                                                                                                                                                                           <  1:773284/239‑1 (MQ=255)                                               tCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCCGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GGGAC‑G‑ACCCGAC‑AAAACCCCGCGAAACACCGCCACATTCCCGGCAAAAGGAtt                                                                                                                                                                                             >  2:265108/1‑238 (MQ=255)                                                            cTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGa                                                                                                                                                                                                      >  1:111364/1‑216 (MQ=255)                                                               tAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTAAGCAGTTCAAACGGGCGCACCGCCGGGGTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGCC‑AATACCCAGCGAATCACCGCCATGGTTCCGGACAATGGATTCCAGCAGCACTGCcgcg                                                                                                                                                                            >  1:1035995/1‑239 (MQ=255)                                                                      gTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCCGGCAGCATAAAACATTGTG‑ACAACCCGGGCGCCGAATATCCCCCC‑A‑GTGAC‑G‑AACCGGA‑AATACCCAGCCAACACCCGCCCTTTTCCAGGACAAAGGATTCcagacacccagccggggcgggag                                                                                                                                                                     >  2:661506/1‑219 (MQ=255)                                                                       tAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AAAACCCACCGAATACCCCCCATGTTCCCGGCCCATGGATTCCAGCAGCCCTGCCCCGTCGGAAgg                                                                                                                                                                    >  1:107076/1‑239 (MQ=255)                                                                       tAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GGGAC‑G‑AACCGAA‑AAAACCCACCGAAACACCGCCATGTTCCAGGACAATTGGTTCCAGCAGCACGGCCGCGCGGGaaag                                                                                                                                                                    >  2:602098/1‑237 (MQ=255)                                                                              ggCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGTTTCCAGCAGCCCCGCCCCGCCGCAACGCTAccg                                                                                                                                                              >  1:1091686/1‑236 (MQ=255)                                                                              ggCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GGGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCCTGTTCCAGGGCAATGGATTCCAGCAGCCCTGCCCCGCCGGAAGGAAAcggc                                                                                                                                                             >  1:598290/1‑239 (MQ=255)                                                                                cGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATTAACGCCATGTTCCAGGACAATGGATTCAAGCAGCTCTGCCGCGTCGGAAggtaacgccg                                                                                                                                                            >  1:227228/1‑230 (MQ=255)                                                                                cGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCcag                                                                                                                                                                                         >  1:494612/1‑209 (MQ=255)                                                                                cGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcggcg                                                                                                                                                            >  1:724712/1‑238 (MQ=255)                                                                                  ggtttgtcgcaatttttGCGGGCCGGTTGAGGGTTTCTCGTGGCGAGCGAAAGGGTCAGTCAGAAGTAAAACGGGGCGACACGGGGGGTTT‑CCCACCAG‑TGCGGGCGGCAAAAAAAATGGTG‑ACAACCAGTGGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACAGAA‑AATACCCAGCGAGTCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCa                                                                                                                                                         <  2:1091686/224‑1 (MQ=255)                                                                                      ctaaccacaTGTGGCTGCCCGGTGTGGGGGTTCCGGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGGGCACCGCGGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTg                                                                                                                                                     <  1:602098/234‑1 (MQ=255)                                                                                          gCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCACCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCCCTGCCGCGCCGGAAGGATAAGGCGGGAAACGCGGa                                                                                                                                                 >  2:640939/1‑239 (MQ=255)                                                                                           ggtttataagagaagggggggggggTTCTCGTTAGGCCCGGGATGGTCCGGCCGAAGTTAAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑GGCGGGCAGAATAAAACATTGGG‑ACAACCAGGGGGCGAAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGa                                                                                                                                                 <  2:757323/217‑1 (MQ=255)                                                                                                ttgtccgcCGGGTGTGGGGTTTCTCGTTGTGCCCGAGAGTGCCCGGGAGCAGTTAAAACGGGCCCCCCACCGGTTTT‑CCCCCCAC‑TGCGGGCAGCAAAAAACATTGAG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGatc                                                                                                                                           <  2:598290/233‑1 (MQ=255)                                                                                                atTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGCCAATGGATTCCAGCAGCACTGCCCCGCGGGAAGGATACGGCGGGAAACGCGGaaataca                                                                                                                                           >  2:1294808/1‑234 (MQ=255)                                                                                                  tgctgcCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGACACTGCGGaaagtac                                                                                                                                           >  1:791991/1‑232 (MQ=255)                                                                                                    ctgcCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCCCTGCCGCGCCGGAAGGATACGGCGGCAACCGTGGGATGatcattc                                                                                                                                       >  1:918222/1‑237 (MQ=255)                                                                                                        ccGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATGTCA‑Ac                                                                                                                                  >  1:683420/1‑239 (MQ=255)                                                                                                            tgtAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTTCGGCATTGTCATCTCA‑CCGTCa                                                                                                                              >  1:1207891/1‑239 (MQ=255)                                                                                                            tgtAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAACGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGCCGGAAGGATACGGCGGCAACCCCGGAATGacacactcaacgcca                                                                                                                               >  2:261904/1‑225 (MQ=255)                                                                                                            tgtAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AAAACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCTTCGGAAGGATACGGCGGCAACTgagagaggactcactaaagctca                                                                                                                               >  1:496596/1‑217 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGTTACGGCGGCAACTGCGGAAAGATCAtctcctggtccctatacccc                                                                                                                      >  1:757323/1‑222 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATa                                                                                                                                                                 >  1:415563/1‑195 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGGCACCTCGGCATGATCATCTCA‑AAGTCAGGGGGATGt                                                                                                                     >  1:144707/1‑238 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAgggggtggt                                                                                                                     >  1:708995/1‑232 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGCCCGGGGGATGt                                                                                                                     >  1:116429/1‑238 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTCCGGAATGCTCATCTCA‑ACGTCCggggctgcc                                                                                                                     >  1:545220/1‑232 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACAGCGGGATGATCATCTAC‑ACGTCCAGGGGATgg                                                                                                                     >  1:859675/1‑237 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCGCTGCCGCGTCGGAAGGATACGGCGGCAACACCGGAATATTCATCTCA‑ACGCCAGGGAGAGGt                                                                                                                     >  1:1261832/1‑238 (MQ=255)                                                                                                                      ctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGAGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTTGGGAAGGATATTTTCA‑ACGCCAGGGGGATgg                                                                                                                     >  1:460824/1‑237 (MQ=255)                                                                                                                             tGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAAGGATCAGCTCA‑ACGCCAGGGGGGTTTTCCgcca                                                                                                              >  1:422346/1‑238 (MQ=255)                                                                                                                             tGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCAATGTTCCAGGACAATGGATTCCAGCAGCTCTGACGCGTCGGAAGGATAGGGCGGCAACTGCGGACTGATCATCTCA‑ACGTCCGGCTGATGTTTCgccc                                                                                                              >  1:836956/1‑237 (MQ=255)                                                                                                                               gccggagttctCCGTCAGCATTTCAAACGGGCGCCCCGCCGGTTTT‑CCACCCCC‑GGCGGGAAGAAAAAAACATTGTG‑AACACCGGGTGGCGTAATAACCCCCC‑A‑GTGAA‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCgccagcc                                                                                                           <  2:900849/229‑1 (MQ=255)                                                                                                                               cccGAGATAGCCCTCCAGCAGTTCAAACGGGCCCCCCGCCGGGTTA‑CCCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑ACCCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCgccagcc                                                                                                           <  1:1193160/238‑1 (MQ=255)                                                                                                                               aCCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCCGGACAATGGATTCCAGCAGCTCTCCCGCGTCGGAAGGATACGGCGGCAACTCTGGAATGATCATCTCA‑ACGTCAGGGTGAGATTCcgcccgcc                                                                                                           >  1:1317521/1‑239 (MQ=255)                                                                                                                               aCCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCCGGGTGATGTTCCgccagc                                                                                                            >  1:1316802/1‑238 (MQ=255)                                                                                                                                           cGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGa                                                                                                                                                                                                      >  1:1237629/1‑137 (MQ=255)                                                                                                                                             ccaccgtgtCAACCGGGC‑CCCCGCCGGGTTT‑CCCCCCAC‑CTCGGGGCAAAAAAAAAAATGTGAACAACCGGGGGCCTAAATACCCCCCC‑A‑GGGAC‑A‑AACCGAC‑AAACCCCAGGGAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAg                                                                                             <  2:422346/231‑1 (MQ=255)                                                                                                                                               cgcagttcacgggtggccccgcgcgGTTTT‑ACCACCCA‑TGCGGCCAACATAAAAAATTGCA‑AAATCCAGGGGCCGAAATACCCCTCA‑A‑GTGAC‑G‑AACGGAC‑AACACACAGTGATGCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCCACTGCGGAATGATCATCTCA‑ACTTCAGGGGGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                                                                                           <  2:879286/216‑1 (MQ=255)                                                                                                                                                 cagTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑AATCCAGGGGGATGTTCCGCCAGCCAGGTTTTTAAAAAACTc                                                                                          >  1:737904/1‑238 (MQ=255)                                                                                                                                                   gTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGGGATGTTCCGCCAGCCAGGTTTTTAACAAGCTCGCt                                                                                       >  1:420140/1‑239 (MQ=255)                                                                                                                                                            ggccccccgcggGGTTT‑ACCACCAC‑GGCGGGCAACAAAAAACATGGTG‑ACACCGAGGGGGCGTAATACCCCCCA‑G‑GGGAC‑G‑AACCCAA‑AATACCCAGGGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCa                                                                                 <  2:708995/230‑1 (MQ=255)                                                                                                                                                              cgcACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATTCCA‑CCGTCCGGGGGATGTTCCGCCACCCCGTTTTTTAACAAATCCGCtttttcgaaccc                                                                            >  1:275492/1‑231 (MQ=255)                                                                                                                                                              cgcACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAAGGATCATCTCa‑a                                                                                                                                   >  1:860107/1‑184 (MQ=255)                                                                                                                                                              cgcACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GCGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCCCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGGTTTTTAACAAGCTCCCTTTTGCagacgc                                                                            >  1:384622/1‑236 (MQ=255)                                                                                                                                                                 cccccccggtgttccctccac‑GGGGGGCAGAAAAAAACATTGGA‑ACACCCAGGGGCGGCAATACCCCCCC‑A‑GTGCA‑G‑AACCGCA‑AATCCCACGGGAACCACCGCCCTGTTCCAGGACAATGGTTTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGcgcg                                                                          <  2:107076/222‑1 (MQ=255)                                                                                                                                                                    ggcgGTTTT‑ACCCCCAC‑GGCGGGCACCATAAAACATTGTG‑ACAACCGGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑ACCCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGa                                                                      <  1:1222033/237‑1 (MQ=255)                                                                                                                                                                    gccgttttt‑ACCCCCCC‑TGCGCGAAGCAAAAAACAGTGTG‑CACACGTGGTGGCAAAATTCCCCACC‑ATGTG‑C‑A‑CACCGAC‑ATTGCCCAGGCAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCCCTGCCGCGTCGGAAGGATACGGCGGCAACTGCTGAATGATCATTTCA‑ACGTCAGGGTGATGTGCCGCCAGCCATTTTTTTAACAAGCTCGCTCTTGCAGAGCTCGGCGa                                                                      <  2:986330/233‑1 (MQ=255)                                                                                                                                                                     gcgGGTTT‑TCCCCCCCGTGGGGGAATCAAAAAACCGTGTG‑ACAACCGGGTGGGTAAATATCCCCCC‑G‑GTGAC‑A‑AACCGAC‑AATCCCCAGCGACTCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGa                                                                      <  2:460824/238‑1 (MQ=255)                                                                                                                                                                      ggggTTTCACCACCCC‑GGAGGGCAACAAAAAACAATGGG‑ACAGCAGGGGGGCGAAAAATCCACAC‑A‑‑AGAG‑GAAACCGAC‑AATCCCACGCGAATCACCGCCATGTTCCAGGACAAGGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAg                                                                     <  2:360958/238‑1 (MQ=255)                                                                                                                                                                      cgGTTTT‑ACCACCCC‑GGCGGGCAGCATAAAACATTGGG‑ACACCCGGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑ACCCGAC‑AATCCCCAGCAAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAg                                                                     <  1:811469/238‑1 (MQ=255)                                                                                                                                                                      cgGGTTT‑CCCACCCA‑TGCGGCCAGCATAAAACTTGGTG‑ACACCCGGGTGGCGAAATTCCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGc                                                                    <  1:572640/239‑1 (MQ=255)                                                                                                                                                                      cgGGTTT‑ACCACCAT‑GGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATACCCCCCC‑A‑GGGAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGc                                                                    <  1:261904/239‑1 (MQ=255)                                                                                                                                                                                ccccat‑TGCGGGCACAATAAACCGTTTGG‑ACACCCAGGGGGCGTAACTCCCCCCC‑A‑GGGAC‑G‑AACCGAA‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAAc                                                           <  1:168032/237‑1 (MQ=255)                                                                                                                                                                                   cac‑TGCGGGCAGCAAAAAACATTGGG‑ACAACCAGGTGGCGTAAGTCCCCCCC‑A‑GGGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCgt                                                        <  1:265108/239‑1 (MQ=255)                                                                                                                                                                                    catggCGGGCAGAATAAAACTTTGTG‑ACAACGGGGTGGCGTAATATCCCCCG‑G‑TGGAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCgt                                                        <  1:970702/235‑1 (MQ=255)                                                                                                                                                                                     c‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACGGCGGGATGATCATCTCA‑ACGTCAGGGTGATGTTTCGCCCGCCAGGTTTTTAACAAGCCAGCTTTTGCAAAGGGCGGGGCGCTGTTGGAAcgcggt                                                      >  2:89522/1‑234 (MQ=255)                                                                                                                                                                                      ccccggccacaaaaaaaCATTGTG‑AACCCGTGGGGGGATAATACCCCCCC‑A‑GGGAC‑G‑ACCCGAC‑AATCCCCGGCGAACCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCgtgt                                                      <  2:1207891/226‑1 (MQ=255)                                                                                                                                                                                      cagggggCAGAATAAAACATTGCG‑ACCACCGGGGGGCGTAAAACCCCCCC‑A‑GTGAA‑G‑AACCGAA‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCgtgt                                                      <  1:794799/235‑1 (MQ=255)                                                                                                                                                                                          cggCAGCAAAAAACATTGAG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GGGAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTACCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGtaaa                                                   <  1:1093989/237‑1 (MQ=255)                                                                                                                                                                                           ggCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGGGATGTTCCGCCAGCCAGTTTTTTAACAAGATCGCTTTTGCAGGAGGCGGCGAGCTGTTGAAACCCGGtaaata                                                 >  1:1023950/1‑239 (MQ=255)                                                                                                                                                                                             gcagaaaaaacatttgggcaaaCCAGGGGGCGTAATCTCCCCCC‑A‑GGGAC‑G‑CACCAAC‑AATACCCAGGGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGtaaata                                                 <  1:582229/218‑1 (MQ=255)                                                                                                                                                                                              aaaaaaaacCATTGTA‑CCAACCGGGTGG‑GTAAAACGCCCCC‑A‑GTGAA‑G‑AAACGCCAAACACCCAGCGAACTACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCTCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAaag                                              <  2:859675/234‑1 (MQ=255)                                                                                                                                                                                                 aaaaaaCATTGTG‑ACAACCGGGTGGCTTAATCTCCCCCC‑A‑GTAAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAaagaa                                            <  1:294660/236‑1 (MQ=255)                                                                                                                                                                                                      catttGTG‑CAAACCAGGGGGCGAAATCCCCCCCC‑A‑GGGGC‑G‑AACCGAC‑AATGCCCACCGAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAg                                      <  2:1316802/236‑1 (MQ=255)                                                                                                                                                                                                          tgtg‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAAAGAACATCTCACACG‑CAGGGGGAAGTTCCGCCAGCCAGTTTTTTAACAAACCCGCTTTTGCAGAGCGCGGCGCGCTGTTGAAACCGTGTAAATAAAAAAGCGtgaaaaa                                  >  2:1029956/1‑233 (MQ=255)                                                                                                                                                                                                                tttgtttaaagcgaacacccccccc‑C‑ATGAA‑C‑AACCGAC‑AAACCCCCGCGAATCCCCGCCATGTTCCCGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTa                             <  2:1265190/220‑1 (MQ=255)                                                                                                                                                                                                                cacccggggggggTAATTCCCCCCG‑A‑GAGCA‑G‑AACCGGA‑AATACCCAGCAAAGCGCCGCCATGGTCCAGGAAAATGGATTCCAGCAGCTCGGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTa                             <  2:1317521/227‑1 (MQ=255)                                                                                                                                                                                                                cAACCAGGTGCCGAAACACCCCCCC‑A‑GGGAC‑G‑AACCGCC‑AATACCCAGCGAACCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTa                             <  1:294183/239‑1 (MQ=255)                                                                                                                                                                                                                acaccggggtgCGTATTCTCCCACC‑T‑GTGCC‑G‑AACCGAA‑AATCCCCGGCGAAGCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTa                             <  2:918222/229‑1 (MQ=255)                                                                                                                                                                                                                aaaCCGGGTGGCGTAA‑ATCCCCCCAA‑GGTAA‑G‑AACCGAA‑AATCCCCAGCGAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTa                             <  1:1029956/238‑1 (MQ=255)                                                                                                                                                                                                                aaaCCAGGGGGCGTAATACCCCCCC‑A‑GGGAC‑G‑AACCGAC‑AATACCCAGGGAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGGCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTa                             <  1:1353561/238‑1 (MQ=255)                                                                                                                                                                                                                           cttaattccccccc‑A‑GGGAC‑G‑AACCAAA‑AATCCCCAGCCAACCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGaa                   <  2:144707/230‑1 (MQ=255)                                                                                                                                                                                                                           cctattgccccccc‑G‑GTGAC‑G‑ACCCACA‑AACCCCCACGGAATCACCGCCATGTTCCAGGAAAAGGGTGTCCAGCAGCCCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGaa                   <  2:387224/230‑1 (MQ=255)                                                                                                                                                                                                                            tgaaTACCCCCCC‑A‑GGGAC‑G‑AACGGAA‑AATCCCCGGGAAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  1:1342011/236‑1 (MQ=255)                                                                                                                                                                                                                            gTAATACCCCCGC‑A‑GGGAC‑G‑ACCCAAC‑AATACCAAGCGAACCACGGCCATGTTCCAGGACAATGGAGTCCAGCAGCTCTGCCGCGGCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  1:294420/238‑1 (MQ=255)                                                                                                                                                                                                                            gTAATACCCCCCC‑G‑AGGAC‑G‑AACCGAC‑AATACCCGCGGAACCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  1:748610/238‑1 (MQ=255)                                                                                                                                                                                                                            gTAATACCCCCCC‑A‑TGGCC‑G‑AACCGAC‑AAGCCCAAGCGAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATCCGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  1:303562/238‑1 (MQ=255)                                                                                                                                                                                                                            gTAATACCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATCCCCAGCGAATCACCGCCATGTTCCAGGACAATGGTTTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  1:919996/238‑1 (MQ=255)                                                                                                                                                                                                                             tcatatCCCCCC‑C‑GTTACAG‑ACCCGAC‑AATCCCCCGGGAAACACCGCCAGGTTCCAGGACAAGGGATTCCAGCAGCTCGGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  2:724712/236‑1 (MQ=255)                                                                                                                                                                                                                             tAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGGAAGATCATCTCA‑ACGTCGGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAACGCGCTTTTGCAGAGCGCGGCGGGATGTTGAAACCGCGAAAAAAAAAAAACGgagaaaaaagaagactttgtacgcg                >  2:642014/1‑218 (MQ=255)                                                                                                                                                                                                                             gaaaatCCCCCC‑A‑GTGACAA‑AACCCAA‑AACCCCCAGGCACACCCCGCCAAGTTCCAGGAAAAGGGGTTCCATGAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCAGCTCA‑ACGTCAGGGTGATGGTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAg                  <  2:300581/234‑1 (MQ=255)                                                                                                                                                                                                                                 atCCCCCC‑G‑GTGCA‑C‑AACCGAC‑AATACCCACGGATACACCCGCATGGTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATGCGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTAt            <  1:169720/239‑1 (MQ=255)                                                                                                                                                                                                                                 acccccca‑A‑GGGAC‑G‑AACCGAC‑AATACCCAGGGAATCCCCGCCATGTTCCAGGCCAATGGATTCCAGCAGCCCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTAt            <  1:642014/237‑1 (MQ=255)                                                                                                                                                                                                                                  acccccc‑A‑GGGACAC‑ACCCAAC‑AATCCCCCGGGAACCCCCGCCATGTTCCAGGCCAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTAt            <  2:1013834/238‑1 (MQ=255)                                                                                                                                                                                                                                   cccccc‑A‑TGGAC‑G‑CACGGAC‑AATCCCCAGGGAATCCCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGAGCATCTCA‑ACGTCAGGGTGATGGTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCa          <  1:585571/239‑1 (MQ=255)                                                                                                                                                                                                                                     cccc‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCC‑ACGGCCGGGGGATGTTCCGCCAGCCAGGTTTTTAACAAGCCTCCTTTTGCCGAGGCGGGCGGGCTTTTGAAAACCGGTAAATAAAAAAACGAAGAAACAGGGAGCCTTTTGaaagggaaacaag        >  2:1183010/1‑228 (MQ=255)                                                                                                                                                                                                                                     cccc‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGGTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGGGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGGATCAgg        >  1:300581/1‑239 (MQ=255)                                                                                                                                                                                                                                             tGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCCGGGTGATGTTCCGCCAGCCCGGTTTTTAACAAGCCCGGCTTTGCCGAGCGCGGCGAGCTGTTGAAACCCGGTTAATAAAAAAAGGTAAGAAACCGTAGCCTTCTTAAAGGGGATAAGGTgggga  >  2:1044682/1‑237 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   TCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTT‑ACCACCAC‑TGCGGGCAGCATAAAACATTGTG‑ACAACCAGGTGGCGTAATATCCCCCC‑A‑GTGAC‑G‑AACCGAC‑AATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCA‑ACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAA  >  NC_000913/3175567‑3176036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑