Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,436 G→A 23.0% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4360GA23.0% 112.3 / 10.4 71intergenic (‑182/‑25)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (5/44);  new base A (2/13);  total (12/59)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTC  >  NC_000913/3206206‑3206665
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
tttCTCCCAGTTCCTTCAATTTTTTTGCTAAAGGCCAGCTATTCCGCTTCGTTAATTTTCCGCCGTTGTGATAAACTCAATGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                                                                                                                            <  2:289336/239‑1 (MQ=255)
tttCTACCAGTTCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                                                                                                                            <  2:333443/239‑1 (MQ=255)
 tttctcctgttccTTCAAGTTTTTGGCTAAAGGCCATGTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                                                                                                                           <  2:611757/228‑1 (MQ=255)
 ttCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCCTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTACGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                                                                                                                           <  1:787489/239‑1 (MQ=255)
  ttctcccgttccTGCAAGTTTTTGGCTAAAGGCCAGCTTTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaa                                                                                                                                                                                                                                          <  2:564283/229‑1 (MQ=255)
         gTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCCAGcaaaaacaaaaaactg                                                                                                                                                                                                                                  >  1:74446/1‑235 (MQ=255)
          tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAGAATTTTTTAAAAAAAAACaaaaaaaaa                                                                                                                                                                                                                                  >  2:125472/1‑235 (MQ=255)
           aCCTGGAAGTCGTTGGCTAAAGGCCAGCTATTAAGCATCGTTAATTAACCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                                                                                                                <  1:759792/237‑1 (MQ=255)
             cTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAACTTTTTAGTGGTAGTTTGTGGGGGGGGAATTTTTTCCACAaaaaaaaaaaaaagaggt                                                                                                                                                                                                                                >  2:1225495/1‑233 (MQ=255)
                 aaGTTTTTGGGTAAAGGCCAGCTATTCAGTATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGcc                                                                                                                                                                                                                       <  1:596525/238‑1 (MQ=255)
                           cttaaGGCCAGCTATTCAGAATCGTTAATTATCCGCAGTTGTGATGAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATGCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                           <  2:394347/236‑1 (MQ=255)
                            tAAAGGCCAGCTTTTCAGCATCGTTAATTTTCCGCCGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                           <  2:74446/238‑1 (MQ=255)
                                   caagttatCAGCATCGTTAATTAACCGCAGTTGTGATAAGCGCAGGGTATTTCGCAAATCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                  <  1:908290/231‑1 (MQ=255)
                                   cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                  <  1:1068602/238‑1 (MQ=255)
                                    aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTCGATTTTTTTCAAAAAATAAAAAAAAAAGAGTTgtcctgcagaggggataaacaag                                                                                                                                                                                                    >  1:962543/1‑217 (MQ=255)
                                    aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACTCAAAAACAAAAAATAGAGTTTGCATGATCAGCGGAAGaaaaaa                                                                                                                                                                                                >  1:990749/1‑239 (MQ=255)
                                       ttttCAGCATTGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                                                                                                                                <  2:1060269/234‑1 (MQ=255)
                                         ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGAGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                          <  1:1324971/239‑1 (MQ=255)
                                               agcGTTAATTAGCCGTAGTTGTGATAAGCGCAGTGTAATTCACAAAACATTGCCTTTCCCTGCTCTGGCCCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGTTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                      <  1:194731/234‑1 (MQ=255)
                                               aacGTTAATTATCCGCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                                                                                                                    <  1:226037/236‑1 (MQ=255)
                                               aTCTTTAATTTTCCGCAGTTGTGATAAGCGCAGTGTATTTCGAAAAACCTTGCCATTCCCTGCTTTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                      <  2:1094915/236‑1 (MQ=255)
                                                tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTGATTTTTTTAACCAAAAAAAAAAAAAGAAGTTTTCCTGATGAGGGAAAGaaaaaaaaaaaaagcaat                                                                                                                                                                                    >  1:377478/1‑227 (MQ=255)
                                                 taaaagacagccGAAGTTGGTATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                                                                                                                 <  1:573161/229‑1 (MQ=255)
                                                   ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCAACAAAAACAAAAAATAGAGTTGCCAAGATTAGTGAAAGtaaataagaaaaaagagagga                                                                                                                                                                                 >  1:796039/1‑221 (MQ=255)
                                                                 ttGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACt                                                                                                                                                                                                                                   <  1:1075336/181‑1 (MQ=255)
                                                                  tgttATAAGCGCAATTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                <  1:403780/238‑1 (MQ=255)
                                                                  tggtaTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                <  1:125472/234‑1 (MQ=255)
                                                                        aagcgccttttttttCGCAAAACATTGCCATTCCCTGCTCTGGCCCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGc                                                                                                                                                         <  2:708906/227‑1 (MQ=255)
                                                                                     taaaagggaccgtcccATTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGGGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAATTTTTCCAGACAAATACAAAACCTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGcc                                                                                                                                          <  1:963453/225‑1 (MQ=255)
                                                                                       caaaaacctttgctttccccTTCTGTGGCACAATTTTCTTTTTTGTTTCCGCGTTTATTACCGATGTTTTAATAACATATCCCGGGGGAAAAAAAATAAAAAACAACATTTTTGGGGGTAGTGTGTGGGGGTAATTTTTTCAAAAAAAAAACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAACAAACAGGCAGTAAAAAAGTTGACAGAGTTTGTCGCAATCTTTACCGCCAt                                                                                                                                        <  2:984749/222‑1 (MQ=255)
                                                                                                         ccgttttgggccaatttttatttcTTATTTCCCTGTTGATGCAAGAACTTTTAATAACATATTCAGGGGGACAAAAAATAAAAACCAATATTTGTAGTGGTAGTGTGGGCCGGTAATTTTTACCAGAAAAATACAAAAACTGGATTGGCCTGTATGAGCAAAAGTAATAAACAACGGGCAGTAAATAGGTGAACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                                      <  2:657469/218‑1 (MQ=255)
                                                                                                             tgttgtgatgttagtttttttttttttCGTGTTGATTCACGAACTTTTAATATCATTTTCACGGGTAAAAAAAATACAAAACCCAATTTTTAGGGGAAGTGGGGGGCGTTGATTTTTTCAAGAAAAATAAAAAAACTGGGGTTCCGATGATGAGCAAAAGAATAAAGCAACAGGCATTGAATAATTTGACATGTATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                                                                                                                  <  2:897400/216‑1 (MQ=255)
                                                                                                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAACAAAAAAAAGAGTTGTCAATGTGAGCGGAAAAAATAAGAAAAACGCAGAGAATAAAATTACAAAGAATTTCGCCAACATTATCCCCCAGATTTTTTCCCCAATAccctagtacgg                                                                                                             >  1:279734/1‑230 (MQ=255)
                                                                                                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGCCAAAAACAAAAAATTGAGGTGCCATGATGAGGGGAAGGAATAAAGAACAGGGCGCGAATAAAATTACAGAGAATTTTGCTACACTTTCCGCCCAGCTTTTTTCCCCCAAGCCtgatggagg                                                                                                             >  1:234470/1‑235 (MQ=255)
                                                                                                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGATTAGCGGAAGTAATAAAGAAAAAGCAGTGAAAAAATTTAAAGAGATTGTTGCTAAATTTACCGCCCTTATTTTTTTCCCAATGCCCGATGACgt                                                                                                             >  1:926801/1‑239 (MQ=255)
                                                                                                                    caaaTTTTCATTATTTTTTTGGAGTTTATCCAAGAATTGTTAATTAAATAGTCGGGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGGTGTTTGTGGTGGTTAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                            <  1:636971/236‑1 (MQ=255)
                                                                                                                     gaaatttttttttttttttCGGGTTTGATTCACGAACTGTTATTTACATATTCACGGTGGAAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                            <  1:866141/223‑1 (MQ=255)
                                                                                                                        ttttcatttTTATTTTCGGGTTTATTCACGAACTTTTAATTAAATATTCACGGTAACAAAAAATATAAACCCACATTTTTAGTGTTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATACAAAAACTGGAGTTACCATGATGAGCAAAAGTAATAAGCAACAGGCTGTGAATAATTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                       <  1:94493/237‑1 (MQ=255)
                                                                                                                        tatTCTTTCTTATTTCCGCGTTGATTCACGAATTTTTATTTACATATTCACGGTGGAAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTGGTGGGGGTAAATTTTTCCAGACAAATAAAAAAATGGGAGTTGCAATGATGAGAGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATGGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                       <  1:633427/239‑1 (MQ=255)
                                                                                                                        tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                     <  1:251550/164‑1 (MQ=255)
                                                                                                                                 ttATTTTCGGTTTGATTCACGAACTTTTATTTAAATATTCCGGGGGGAAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTCCAAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGAAAAAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                              <  1:1325455/239‑1 (MQ=255)
                                                                                                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCATAAAAAAAAAAAAAACAGGGTTGCCATGATTAGGGGAAGGAAAAAACAAACAGGAGGGGAAAAATTTGCCAGAGATTTTGGCTAATTTACAGCCATGATTTTTAACCCGATGCCtggttggagggggggtaaaacaaaa                                                                                             >  1:230555/1‑216 (MQ=255)
                                                                                                                                  tATTGTCGCTTTGATTCACAAACTTTAAATTACATATTCAGGGTGGAAAAAAATAAAAAACCACATTTTAAGTGTTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                             <  1:185922/239‑1 (MQ=255)
                                                                                                                                             tgaatgccggAACTGTTAATTACTGATCCGTGGGTGAAAAAATATAAAACTCACATTTTTAGTGGGTGTTTCGGGGCGTAAATTTTTCAAGACAAAGACAAAAGCTGGGGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGGTGTGAATAAGTTGACGGAGGTTTTCGCTAATTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAgg                                                                                   <  1:866476/230‑1 (MQ=255)
                                                                                                                                                        atttttAATTTCATTTGCGCGGGGTAAAAAAAAAAAAAACCACAATTTTGGTGGTGGGTTGGGGGCGGTAAATTTTCAGAGAAAAAAAAAAAAACGGGGTTTGCAATGATGGACAAAATTAAAAAGCAACAGGCAGTAAATAAGTGAACAGAGTTTGGCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAc                                                                         <  2:125298/235‑1 (MQ=255)
                                                                                                                                                            ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                           <  1:735271/111‑1 (MQ=255)
                                                                                                                                                                   aatatttctggggggaaaaaaTAAAAACCCATATTGTGAGGGGGATTTGGGGGGGGGAATTTTTTCCAAAAAAAAACAAAAAGTGGAGTGGCCATGATGAGCAAAAATAATACGCAACAGGCAGTAAAAAAGTTGACAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                             <  2:261179/224‑1 (MQ=255)
                                                                                                                                                                     ttttccggggtgaaaaaaaTAAAAAACCATATTTTAAGTGGGAGTTTGTGGCGGTAAATTTTGCCAGAAAAACACAAAAACTGGAGTTGCTAGTATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAGTTTGACAGATATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                           <  1:687311/226‑1 (MQ=255)
                                                                                                                                                                     ttttccgggTGGAAAAAAAAAAAAAACCAATTTTTAAGGGGTATTTTGGGGCGGTAAATTTTTCCAGAAAAATACAAAAAGTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                           <  1:1162756/232‑1 (MQ=255)
                                                                                                                                                                     tattacgggTGGAAAAAAAAAAAAAACCAATTTTTGAGGTGTATTTTGTGGCGTGTAATTTTTCCAAAAAAATAAAAAAACGGGGGTTCCCATGAGGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                           <  1:865615/232‑1 (MQ=255)
                                                                                                                                                                      tttttggggcggaaaaaaTTAAAAACCAAAATTTTTGGGGTGTGTTGGGGGGGGTAATTGTTCAAAGAAAAATACAAAAGTGGGGGTGGCCTGGGTGACGAAAGTTAATAAGCAACAGGATGGAAATAAGTTGAAAGAGTTGTTCGCTATTTTGACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                           <  2:114802/227‑1 (MQ=255)
                                                                                                                                                                      tttccggggtgCAAAAAAAAAAAAACCAATATTTGTAGGGGGATGTGGGGGGGGGTAATTTTGCAAAAAAAATACAAAAATGGGAGTTGCCATGATGAGCAAAAGTAAAAAGAAAGAGGTAGTAAAAAATTTGACAGAGATTGGCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                           <  2:918099/229‑1 (MQ=255)
                                                                                                                                                                      tttccaggggtaaaaaaaaaaaaaaaCCTATTTTTGGTGGGTGTTTTGGGGGGGAAAATTTTCCAAAAAAAATACAAAAACGGGATGTGCCATGATGGGCAAAAGAAATAAGAAACAGGCAGTAAATAAGTTGACAGAGATGTCCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                           <  2:926801/227‑1 (MQ=255)
                                                                                                                                                                      atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCAGACAAAACAACAAATAGGCGTACCCATTTTAGGCAAAGTAATAAAAAACAAGGACATAAAAAGTTTGCACAAATTTTCCCCAACCTTAACCCCCAGATTTATACCCCACAACCTGCTACCCGGGTGGGTAAACTTAAACAAATAAAGAATGCCGAAAccaactgctaagtgga                                                          >  1:153408/1‑224 (MQ=255)
                                                                                                                                                                            ggttgaaaaaaaTTAAAAACCTCTTTTTTGGTGGGAGTTTTGGCGGGGGATTTTTCCCAGAAAAAAAAAAAACTGGGGTTGGCAATGAGGGGCGAAAGTAAAAACAACAGGGCATGGAAAAGGTTAAAAAGGATGGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAGTAt                                                    <  2:1049790/233‑1 (MQ=255)
                                                                                                                                                                            gggggaaaaaaaTTTAAAACACATTTTTTGTGTGGGTGTTGGGGGGGGGAAATTTTCCAAAACAAATAAAAAACTGGGGGTGACAAGGAGGGGCAAAAGTAAAAACAAAGAGGCAGTAAATAGGTTGACGGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTa                                                     <  2:377478/233‑1 (MQ=255)
                                                                                                                                                                            ggggaaaaaaaaTAAAAACCCAAATTTTTAGTGTTATTTTGGGGCGGAGAATTTTACAAGACAAATACAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                    <  1:1366435/233‑1 (MQ=255)
                                                                                                                                                                             ggggacaaaaaTATAAAACCCCATTTTTTGTGGGTAGTTGTGGGGGGTAATTTTTTCAAAAAAAAAACAAAAACTGGAGTTGCCATGATGAGCAAAAGAAATAAGAACAAGGCGTTCAAAAGTTTGACAGAGTTTTCCTCTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                    <  2:990749/233‑1 (MQ=255)
                                                                                                                                                                             gggaaaaaaaaTAAAAAACCCCATTTTTGGTGGTAGTTGGTGGGGGTGAATTTTCCCAGAAAAATAAAAAAATTGGAGTTCCCATGATGAGCGAAAATAATAAGCAAGGGGCAGTAAATAGGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                    <  1:72896/233‑1 (MQ=255)
                                                                                                                                                                             gTGGAAAAAAATATAAAACCACATTTGTAGGGGTAGTTTGGGGGGGTAAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                    <  1:906440/238‑1 (MQ=255)
                                                                                                                                                                                    aaatTATAAAACACAATTTTTGTTGTAATTTTGGTGGGTTAAATTTTCCAAACAAAGAAAAAAACCTGGTGTTCCCATAGGGGGCAAAGGTAAAAACCAACGGGAGGTGAATAGTGGAAGAGAGATGTTGGCTAATTTTACCGACATGATTTCTACCCGAATGCCTGAGGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                            <  2:553847/239‑1 (MQ=255)
                                                                                                                                                                                    aaaaTAAAAAACCAAATTTTTGGGGGTAGTTGGTGGCGGTAAATTTTTCAAAAAAAATAAAAAAACGGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                            <  1:1238938/239‑1 (MQ=255)
                                                                                                                                                                                    aaaaTAAAAAACCAAATTTTAGGTGTGAGTTGGGGGCGGTAAATTTTACAAGAAAAATAAAAAAACTGGATTCGCCATGGTGAGCGAAAGTAATAAGCAACAGGCAGTAAAAAAGTTGACAGAGATGTCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                            <  1:970808/239‑1 (MQ=255)
                                                                                                                                                                                               ccACATTTTGAGGTGTAGTTTGGGGGGGTAAATTTTTCCAGAAAAAAAAAAAAACTGGATTTGCAATGATGAGAGAAAGTAAAAAGCAAAAGGAAGTGAATAAGTTGAAAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                 <  1:768164/239‑1 (MQ=255)
                                                                                                                                                                                                   tattttttAGGGGTATTTTGGGGGGGTGAATTTTCCCAAACAAACACAAAACGTGGATTCCCAATGATGAGCAAAAGTAATAAGCAACAGGAAGTAAATAAGTTGACAGAGATTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                               <  1:1212762/236‑1 (MQ=255)
                                                                                                                                                                                                            gTGGTTGTTTGGGGGGGTGAATTTTTCCAAAAAAAAAAAAAAACGGGAGTTCCCAGGAGGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGAAAGGGTTTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                      <  2:750034/239‑1 (MQ=255)
                                                                                                                                                                                                                  gtggatgttgggggggggaaaTTTTTCCAAGAAAATAAAAAACCGGGAGTTGCCATGATGAGCAAAAGAAAAAAGCAAGAGGGAGTGAATAAGTGGACAGAGATTCCCGTTAGTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                      <  1:320340/220‑1 (MQ=255)
                                                                                                                                                                                                                      gttgggggCGGTGAATTTTCCAAGACAAAAAAAAAAGCTGGAGTTGCCAGGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTGGAAAGAGTTTGACGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                 <  1:108547/232‑1 (MQ=255)
                                                                                                                                                                                                                          gggggggTGAATTTTCAAATAAAAATACAAAAACTGGAGTTGCCAGGAGGAGAGAAAGAAATAAGCAACAGGAAGGAAATAAGTTGACAGAGATGGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc            <  1:474483/234‑1 (MQ=255)
                                                                                                                                                                                                                                    gtaattttttCCAAAAAAAAAAAAAAATGGGGATGCCATAAGAGACGAAAATAAAAAAGAAACGGGAAGTAAAAAAGTCAAGAGATATTGTCCTAACTTTTACCGGCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGaa       <  2:234470/233‑1 (MQ=255)
                                                                                                                                                                                                                                         attttCCAAACAAAAACAAAAACTGGGATTTGCCTTGAGGAGCAAAAGTAAAAACCAAAGGGCAGGGAATAGGTGGCAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc  <  1:758491/238‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
TTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTC  >  NC_000913/3206206‑3206665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: