Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,499,594 G→T 19.1% C50F (TGC→TTC)  yhfL → small lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,5940GT19.1% 115.2 / 2.2 70C50F (TGC→TTC) yhfLsmall lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (18/37);  new base T (12/3);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 2.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTAT  >  NC_000913/3499362‑3499825
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
tCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCgg                                                                                                                                                                                                                                                        <  1:1026718/236‑1 (MQ=255)
          ttCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCACTTTCTATTTCTAAAATCATTGGCGGGTGTGGTcctcctccccc                                                                                                                                                                                                                                            >  2:1026718/1‑233 (MQ=255)
                             ttATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAt                                                                                                                                                                                                                      <  1:142789/238‑1 (MQ=255)
                                                        attaatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAt                                                                                                                                                                                                                      <  1:621338/211‑1 (MQ=255)
                                                                  aataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCCACTTCACAGCAAGTCTCTCACAGAAAACTGGTAAACCCCGGTTTGTTTGCCCACCCCCCCCTggt                                                                                                                                                                      >  1:748088/1‑238 (MQ=255)
                                                                   ataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTCTCGGTCCTACTGTACAGTAAGACTCTGTCAGAAATCGGCTACAAACCGGttcatttcccgcgccaccctggcct                                                                                                                                                                          >  1:1038920/1‑216 (MQ=255)
                                                                    taCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCCACTGCCCACGAAATCTCTGATAGAAAACGCCATAACACGGCTTATATTCCCCACCGcccctggcatt                                                                                                                                                                      >  1:276888/1‑231 (MQ=255)
                                                                         aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCTCTCCCCACACAAGCTCTTACAGAAAACGGCTAAAAACCGGTTAATTTCCCCACCCCCCCTTGGTTTTctcc                                                                                                                                                             >  1:263292/1‑236 (MQ=255)
                                                                         aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGa                                                                                                                                                             <  1:598280/239‑1 (MQ=255)
                                                                         aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGAGGTCCTACTGCACAGTAAGTCTCTCTCAGAAAACGGCTCAAACCGCGTTAATTTCCCCCCCCCCCCTGGGTTTTctca                                                                                                                                                             >  1:292916/1‑236 (MQ=255)
                                                                          gggTATTTAAATAAAAAAATTTAATAAAATTGCAATAGTAGGTCCAGTAATGGCTTATTTAACTCGACTGAATGGTCCTTGTGTAATCCGGTATCAGAGCTGGACTTACGACTGCCTGCTTCTCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCATCTGGATTTTCCGAg                                                                                                                                                            <  2:1129087/239‑1 (MQ=255)
                                                                           ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGTCCTACTGCCCAAGAATATTTCTCACAAAAATAGGTGAAAACGGGTTAATTTCCCGGCGCCCCCTGGGATTTTCCggg                                                                                                                                                            >  2:620975/1‑237 (MQ=255)
                                                                           ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCCACTGACCAACAAGTTCCTGACAGAAAACCGGTAACACCCGGTTCTTTGCCCCACGCCCCCTGGACTTTTCCggg                                                                                                                                                            >  1:790905/1‑237 (MQ=255)
                                                                               tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTg                                                                                                                                                                                                                                                           <  2:1131975/154‑1 (MQ=255)
                                                                               tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTg                                                                                                                                                                                                                                                           >  1:1131975/1‑154 (MQ=255)
                                                                               tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTGCCACTTCCCCGCAAGTCTCTATCCACAATCACCTCAAACACGTTTAATTGTCCGCAGCCCCACTGGATTTTCCCAggcgg                                                                                                                                                        >  1:661939/1‑236 (MQ=255)
                                                                               tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCTGCCCAGTAAGGCTCTGTCAGAAAACGGCTAACACCCGGTTTATTTTCCCACGCCCCCCTGGTTTTTcccgggggg                                                                                                                                                        >  1:128606/1‑232 (MQ=255)
                                                                               tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTACACAGCAAGTGTCTCACCACAAACGGCTAAAACCCCGGTAATTTTCCCACGCCCTCTGGGtttttccaaggggg                                                                                                                                                       >  1:181996/1‑229 (MQ=255)
                                                                                         cAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGCGGCCCACTTACCAGTAAGTCTCTTACAGAAAAACGGCAACAACCGGTTTTATTCCCCCCGCCCCCTGGGTTTTTCCGAGGGGGTATttttttt                                                                                                                                            >  1:1264361/1‑238 (MQ=255)
                                                                                            aTTTTTTTAATTTTCACTGGTAGGTGCAGTACTGGCTTCGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGATAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATg                                                                                                                                        <  2:530601/239‑1 (MQ=255)
                                                                                                 ttaAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGATGCGGTCCTACTCCCCAGTAAATCTTTGACCGGAAAAGGCTAACACCCGTTTAATTTCCCCACCGCCCCTGGGTTTTTCGCGGGCGGAGTTTTTTCTATTTTctt                                                                                                                                   >  1:322958/1‑239 (MQ=255)
                                                                                                                         tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCCCCGATAATTTCTGACAGAAATCGGCTAACACCGGGTTAATTTCCCGCGGCCCCCTGGGTTTTTCCCGGGGGGGTTTTTTTTTTTGTCCTTCTTTCCCCCCCCCCCAAAAAAAtt                                                                                                          >  1:106786/1‑239 (MQ=255)
                                                                                                                              gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACCTCACACTAAATCTCTTACCAGAATCAGCCAACACCCGCTTAATTTTCCGGCCCCCCCCTGATTTTTCCACGGCGTCATTTTTTTTTTTTTCTTCTTTCCCCCCCCCCTcaaacaaatttt                                                                                                       >  2:144019/1‑229 (MQ=255)
                                                                                                                              gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACCGTAAGTCTCTGTCAGAAAACGGCCTACACCCGTTTAATTGCCCCGCGCCCCCTGGCTTTTTCCAGGGCGGATTTGTTTTTTTGTCATTCTTTTCCCCCCCCCTCAAAAAAttttt                                                                                                       >  1:414282/1‑236 (MQ=255)
                                                                                                                              gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGCGCTCCTGCCCAGCAAAACTCTGTGCGAAAAACGCTAACCCCGGCTTTAATTGCCGACGCCCCCTGGAGTTTTCGAGGTGGGATTTTGTTTTTTTGTCTTCTTGTccccccccccacaaaaaattttat                                                                                                     >  2:923716/1‑223 (MQ=255)
                                                                                                                                   gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGTGTCCTTCTCCCCAATAAGTCTCTGCCCGAAAATGGGTAACACCGGGGTAATTGCCCGCACGCCTCTGGAGTTTCCCGAGGGGGCTTTTGTTTTTTTGCCTTCTTTCCCCCCCCCCCGAAAAATTTTTTCCTTTTa                                                                                                 >  1:117935/1‑239 (MQ=255)
                                                                                                                                   gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAATCTCTTACAAAAAATGGCCAAACCCCGTTTTATTTCCCCGCGCCCCCCTGGTTTTTCCAGGGGGGATTTTTTTTTATTTCCTTCTTTGCCCCCCCCCTCAAAAAATTTTTCCTTTaa                                                                                                >  1:982356/1‑239 (MQ=255)
                                                                                                                                    ttAACGTCATGTTCTGGTCATATTGAAAACCGGTATAAGAACTGCTCTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                                               <  2:790905/239‑1 (MQ=255)
                                                                                                                                     tAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                                               <  1:923716/238‑1 (MQ=255)
                                                                                                                                             tgcaCTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCGCACAGCAAATCTTTTCCAGAAAACAGCTTACACCCACTTGATTGCCCGCCCCCCTCTCGGTTTTCCGAGGGGGCGGTTTTTTTTTTTTTCTTCTTTCCCCCCCCCCGCAAAAAAATTTTCTTTTTAATTTAAAttt                                                                                       >  2:266657/4‑239 (MQ=255)
                                                                                                                                              tttcTGGTCATATTGAAAACCGTTATAAGAACTTCTCTTACGGCTTCCTGCTTCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                     <  2:414282/236‑1 (MQ=255)
                                                                                                                                               tACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGa                                                                                                                                                             <  1:213764/169‑1 (MQ=255)
                                                                                                                                               tACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                    <  1:266657/239‑1 (MQ=255)
                                                                                                                                                aCTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                    <  1:54940/238‑1 (MQ=255)
                                                                                                                                                   ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTTCACCATAAATCTCTGTCAGAAAACAGCCATACCCGCGTTTATTGTCCGACGCCCCCTTGAGTTTTCCCGGGGGTGTTTGTTTTTTTTTCTCTCCTTCCCCCCCCCCTCCAAAAATTTTTACTTTtaaataaaatatttaaaa                                                                                >  2:685308/1‑224 (MQ=255)
                                                                                                                                                      cATTTTGAAAACCGTGATAAGAACTGCTCTTACGGCTACCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                              <  2:263292/238‑1 (MQ=255)
                                                                                                                                                       atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTCCCACTGCCCCTGAAGTTACTGACAGAAAACGGCTCACACCCGTTTTATTTCTCTCCGCCCTCCTGGTTTTTCCAAGGGGGATTTGTTTCTTTGAGCTTTTTTGCCCCGCCCGCGCAAAAATATTTACTTTTAATTCAATTTTTTAACTACa                                                                             >  1:251274/1‑238 (MQ=255)
                                                                                                                                                         atTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTAATGCACAGTAAGTCTCTGACAGAAATAGGCTAACACCCGTTTGATTGCCCGACGCCCTCTGGGTTTTCCGATGGGGGATTTGTTTTTCTGTCCTTCTTGTCCCCGCCCGTACAAATATTTTTACTTTAAATTTCATTTGTTAACAACATc                                                                           >  1:1138901/1‑238 (MQ=255)
                                                                                                                                                               aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                    <  1:1225070/239‑1 (MQ=255)
                                                                                                                                                               aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCCCTGCACAGTAAGACTCTGACAGAAAAAGGCTAACACCGGTGTATTTTCCCCGCCCCCCCTTGGTTTTTCCCAGGCGGGTTTTTTTTTTTTTTCTTCTTTCCCCCCCCCCCCAAAAATATTTTTCTTTTaatttaattttttaataaaaaccctcaa                                                                     >  2:1225070/1‑220 (MQ=255)
                                                                                                                                                                 ccGTTATAAGAACTGCTCTTACGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                  <  1:144019/239‑1 (MQ=255)
                                                                                                                                                                    tGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCAATGCACAGTAAATCTCTGACAGAAAACGGCCAACACCGGGTTTATTGCCCGACCCCCCCTTGGTTTTTCGCGGGCGGATTTGTTTTTATTTTCCTCTTTCCCCCCCCCCTCAAAAAATTTTTTCTTTTAATTTAATTTTATaaataaattcctttaccccc                                                                >  1:1323562/1‑226 (MQ=255)
                                                                                                                                                                      aTAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGAc                                                                                                                                                                                   <  1:313549/128‑1 (MQ=255)
                                                                                                                                                                      aTAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                    <  1:1051739/216‑1 (MQ=255)
                                                                                                                                                                      aTAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                              <  1:244579/222‑1 (MQ=255)
                                                                                                                                                                      aTAAGAACTGCGCTTACGACTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                  <  1:879183/234‑1 (MQ=255)
                                                                                                                                                                            aCTGCTCCTGCTACTACCTGCTGCACCCGGCAAGTTCTATTTCTAAAATCATTGGCGGTTGCCGTCCTACTGCACAGTAAGTCTCTGACAGAAACCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                  <  1:99542/212‑1 (MQ=255)
                                                                                                                                                                                ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTCCCCCGGAAGTATTTTATCAAAATCGGCCAAACCCCGCTTTATTTCCCGCCGCCCCCTCGGCTTTTTCAGGGCGTGTTTGGTTTTATGTCCTTTTTTTACCCCCCCTCCATAATATTTTTACTTTTAATTCTATTTTTTTACTACATCCCTTCACcacacacaccgccccc                                                    >  2:879183/1‑226 (MQ=255)
                                                                                                                                                                                ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGGGGGCCCCCTCCCCCCTAAATTTCTTTCAAAAATATGCTAACACCCGGTTTATTTCCCCCCCCCCCCCGGTCTTTTCGAGGGCGGGTTTTTTTTTTTTTTCTTTTTTTCCCCCCCCCCCAAAAAAATTTTTATTTTTATTTAATTTTTTAACTACAccccccttctcttttataaaactta                                                    >  2:1051739/1‑216 (MQ=255)
                                                                                                                                                                                ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGCTTCGCTCCCCCTCGCCATTAAGTTTCTTACAGAAAACGACTCACACCGCGTTAATTGTCCCCCACCCCCCCGAGTTTTCGAGGGCGTCGTTTTTTCTTTTTCCTTCTTCTCCCCCCCCCCCCAAAAATTTTTCCTTTTAATTTAATTTTTTAACTACCTccccttatcctctctcttataaaca                                                   >  2:244579/1‑216 (MQ=255)
                                                                                                                                                                                 tctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCcccgc                                                                                                                       <  1:328563/170‑1 (MQ=255)
                                                                                                                                                                                     aCGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACCCCCCAGGAAGATTCTGTAAAAAAACGGGTAAAACCGGGTTTATTTCCCCGCCCCCCCTCGGTTTTTCCGGGGCGGATTTTTTTTTTTTTCCTTTTTGCCCCCCCCCCCCAAAAAATTTTTCCTTTTAATTTCATTTTATAAATAAACCTCTTAACCACACcgcgccactctgcaaaa                                              >  2:753481/1‑226 (MQ=255)
                                                                                                                                                                                           gccTGCCGCCACCGGCAATTTCTATGTCAAAAATCATTGGCGGTTGCGGGTCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt                                        <  1:675723/238‑1 (MQ=255)
                                                                                                                                                                                                  gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGTCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                 <  1:2026/239‑1 (MQ=255)
                                                                                                                                                                                                   cACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACAGTAAGTCTCTTACAGAAAACGGCTAACACCCGGTTTATTTCCCCACCCCCCCTGGAGTTTTCCAGGGGGGATTTGTTTTTTTTTCCCTCTTTTCCCCCCCCCCAAAAAAAATTTTTCTTTTAATTTAAATTTATAACTACATTCATTTTCCCCACCGCCATCCCTTTCGGAAATTTGTGATCTtc                                 >  1:264811/1‑238 (MQ=255)
                                                                                                                                                                                                    ccccGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                               <  1:620975/238‑1 (MQ=255)
                                                                                                                                                                                                    aCCCGGCAATTTCTATTTCCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                               <  1:790250/239‑1 (MQ=255)
                                                                                                                                                                                                       cGGCAATTTCTATTTTTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                            <  1:573017/239‑1 (MQ=255)
                                                                                                                                                                                                       cGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                            <  1:234096/239‑1 (MQ=255)
                                                                                                                                                                                                         gctatttttttttctAAAATCATTGGCGGTTTCGGTCCTACTGCACAGTAAGTCTTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                          <  2:982356/236‑1 (MQ=255)
                                                                                                                                                                                                         gCAATTTCTTTTTCTAAAATGATTGGCGGTTGCGGGCCTACTGCACATTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCTTATTTGTTTATATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                          <  2:276888/239‑1 (MQ=255)
                                                                                                                                                                                                         gCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGAc                                                                                                                                                                                   <  1:309406/93‑1 (MQ=255)
                                                                                                                                                                                                         gCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                          <  1:753481/239‑1 (MQ=255)
                                                                                                                                                                                                         gCAATTGCTAGTTCTAAAATCATGGGCGGTTGCGGGCCTACCGCACAGTAAGTCTCGGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAGTTGATAACTACATCCATTAGCCACATCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                          <  1:584920/239‑1 (MQ=255)
                                                                                                                                                                                                          cAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGGCTCTGACAGAAATCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                          <  1:170024/238‑1 (MQ=255)
                                                                                                                                                                                                              ttctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACa                     <  1:638748/239‑1 (MQ=255)
                                                                                                                                                                                                               tttttttctaAAATCTTTGGCGTTTGCGGTCCTACTGCCCAGTAAGTTTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                    <  2:322958/235‑1 (MQ=255)
                                                                                                                                                                                                                    ttcttAAATTCTTGGCGGTTTGCGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGTTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcat               <  2:661939/239‑1 (MQ=255)
                                                                                                                                                                                                                     tctaAAATCCTGTGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatc              <  2:292916/239‑1 (MQ=255)
                                                                                                                                                                                                                        aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata           <  1:593032/239‑1 (MQ=255)
                                                                                                                                                                                                                          aaTCATTGGCGGTTTGGGTCCTTCTGCCCAGTAAGTCTTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg         <  2:117935/239‑1 (MQ=255)
                                                                                                                                                                                                                                 ggCGGTTGCGGTCCTACTCCCCAGTAAGTCTCGGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtat  <  2:1323562/239‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
TCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTAT  >  NC_000913/3499362‑3499825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: