Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,499,607 A→C 27.5% A54A (GCA→GCC yhfL → small lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,6070AC27.5% 104.3 / 9.8 71A54A (GCA→GCCyhfLsmall lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (14/36);  new base C (16/5);  total (30/41)
Fisher's exact test for biased strand distribution p-value = 4.33e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCA  >  NC_000913/3499391‑3499843
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                              
ttATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAt                                                                                                                                                                                                                                        <  1:142789/238‑1 (MQ=255)
                           attaatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAt                                                                                                                                                                                                                                        <  1:621338/211‑1 (MQ=255)
                                     aataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCCACTTCACAGCAAGTCTCTCACAGAAAACTGGTAAACCCCGGTTTGTTTGCCCACCCCCCCCTggt                                                                                                                                                                                        >  1:748088/1‑238 (MQ=255)
                                      ataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTCTCGGTCCTACTGTACAGTAAGACTCTGTCAGAAATCGGCTACAAACCGGttcatttcccgcgccaccctggcct                                                                                                                                                                                            >  1:1038920/1‑216 (MQ=255)
                                       taCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCCACTGCCCACGAAATCTCTGATAGAAAACGCCATAACACGGCTTATATTCCCCACCGcccctggcatt                                                                                                                                                                                        >  1:276888/1‑231 (MQ=255)
                                            aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCTCTCCCCACACAAGCTCTTACAGAAAACGGCTAAAAACCGGTTAATTTCCCCACCCCCCCTTGGTTTTctcc                                                                                                                                                                               >  1:263292/1‑236 (MQ=255)
                                            aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGa                                                                                                                                                                               <  1:598280/239‑1 (MQ=255)
                                            aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGAGGTCCTACTGCACAGTAAGTCTCTCTCAGAAAACGGCTCAAACCGCGTTAATTTCCCCCCCCCCCCTGGGTTTTctca                                                                                                                                                                               >  1:292916/1‑236 (MQ=255)
                                             gggTATTTAAATAAAAAAATTTAATAAAATTGCAATAGTAGGTCCAGTAATGGCTTATTTAACTCGACTGAATGGTCCTTGTGTAATCCGGTATCAGAGCTGGACTTACGACTGCCTGCTTCTCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCATCTGGATTTTCCGAg                                                                                                                                                                              <  2:1129087/239‑1 (MQ=255)
                                              ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGTCCTACTGCCCAAGAATATTTCTCACAAAAATAGGTGAAAACGGGTTAATTTCCCGGCGCCCCCTGGGATTTTCCggg                                                                                                                                                                              >  2:620975/1‑237 (MQ=255)
                                              ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCCACTGACCAACAAGTTCCTGACAGAAAACCGGTAACACCCGGTTCTTTGCCCCACGCCCCCTGGACTTTTCCggg                                                                                                                                                                              >  1:790905/1‑237 (MQ=255)
                                                  tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTGCCACTTCCCCGCAAGTCTCTATCCACAATCACCTCAAACACGTTTAATTGTCCGCAGCCCCACTGGATTTTCCCAggcgg                                                                                                                                                                          >  1:661939/1‑236 (MQ=255)
                                                  tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCTGCCCAGTAAGGCTCTGTCAGAAAACGGCTAACACCCGGTTTATTTTCCCACGCCCCCCTGGTTTTTcccgggggg                                                                                                                                                                          >  1:128606/1‑232 (MQ=255)
                                                  tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTACACAGCAAGTGTCTCACCACAAACGGCTAAAACCCCGGTAATTTTCCCACGCCCTCTGGGtttttccaaggggg                                                                                                                                                                         >  1:181996/1‑229 (MQ=255)
                                                            cAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGCGGCCCACTTACCAGTAAGTCTCTTACAGAAAAACGGCAACAACCGGTTTTATTCCCCCCGCCCCCTGGGTTTTTCCGAGGGGGTATttttttt                                                                                                                                                              >  1:1264361/1‑238 (MQ=255)
                                                               aTTTTTTTAATTTTCACTGGTAGGTGCAGTACTGGCTTCGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGATAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATg                                                                                                                                                          <  2:530601/239‑1 (MQ=255)
                                                                    ttaAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGATGCGGTCCTACTCCCCAGTAAATCTTTGACCGGAAAAGGCTAACACCCGTTTAATTTCCCCACCGCCCCTGGGTTTTTCGCGGGCGGAGTTTTTTCTATTTTctt                                                                                                                                                     >  1:322958/1‑239 (MQ=255)
                                                                                            tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCCCCGATAATTTCTGACAGAAATCGGCTAACACCGGGTTAATTTCCCGCGGCCCCCTGGGTTTTTCCCGGGGGGGTTTTTTTTTTTGTCCTTCTTTCCCCCCCCCCCAAAAAAAtt                                                                                                                            >  1:106786/1‑239 (MQ=255)
                                                                                                 gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACCTCACACTAAATCTCTTACCAGAATCAGCCAACACCCGCTTAATTTTCCGGCCCCCCCCTGATTTTTCCACGGCGTCATTTTTTTTTTTTTCTTCTTTCCCCCCCCCCTcaaacaaatttt                                                                                                                         >  2:144019/1‑229 (MQ=255)
                                                                                                 gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACCGTAAGTCTCTGTCAGAAAACGGCCTACACCCGTTTAATTGCCCCGCGCCCCCTGGCTTTTTCCAGGGCGGATTTGTTTTTTTGTCATTCTTTTCCCCCCCCCTCAAAAAAttttt                                                                                                                         >  1:414282/1‑236 (MQ=255)
                                                                                                 gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGCGCTCCTGCCCAGCAAAACTCTGTGCGAAAAACGCTAACCCCGGCTTTAATTGCCGACGCCCCCTGGAGTTTTCGAGGTGGGATTTTGTTTTTTTGTCTTCTTGTccccccccccacaaaaaattttat                                                                                                                       >  2:923716/1‑223 (MQ=255)
                                                                                                      gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGTGTCCTTCTCCCCAATAAGTCTCTGCCCGAAAATGGGTAACACCGGGGTAATTGCCCGCACGCCTCTGGAGTTTCCCGAGGGGGCTTTTGTTTTTTTGCCTTCTTTCCCCCCCCCCCGAAAAATTTTTTCCTTTTa                                                                                                                   >  1:117935/1‑239 (MQ=255)
                                                                                                      gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAATCTCTTACAAAAAATGGCCAAACCCCGTTTTATTTCCCCGCGCCCCCCTGGTTTTTCCAGGGGGGATTTTTTTTTATTTCCTTCTTTGCCCCCCCCCTCAAAAAATTTTTCCTTTaa                                                                                                                  >  1:982356/1‑239 (MQ=255)
                                                                                                       ttAACGTCATGTTCTGGTCATATTGAAAACCGGTATAAGAACTGCTCTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                                                                 <  2:790905/239‑1 (MQ=255)
                                                                                                        tAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                                                                 <  1:923716/238‑1 (MQ=255)
                                                                                                                tgcaCTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCGCACAGCAAATCTTTTCCAGAAAACAGCTTACACCCACTTGATTGCCCGCCCCCCTCTCGGTTTTCCGAGGGGGCGGTTTTTTTTTTTTTCTTCTTTCCCCCCCCCCGCAAAAAAATTTTCTTTTTAATTTAAAttt                                                                                                         >  2:266657/4‑239 (MQ=255)
                                                                                                                 tttcTGGTCATATTGAAAACCGTTATAAGAACTTCTCTTACGGCTTCCTGCTTCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                       <  2:414282/236‑1 (MQ=255)
                                                                                                                  tACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGa                                                                                                                                                                               <  1:213764/169‑1 (MQ=255)
                                                                                                                  tACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                      <  1:266657/239‑1 (MQ=255)
                                                                                                                   aCTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                      <  1:54940/238‑1 (MQ=255)
                                                                                                                      ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTTCACCATAAATCTCTGTCAGAAAACAGCCATACCCGCGTTTATTGTCCGACGCCCCCTTGAGTTTTCCCGGGGGTGTTTGTTTTTTTTTCTCTCCTTCCCCCCCCCCTCCAAAAATTTTTACTTTtaaataaaatatttaaaa                                                                                                  >  2:685308/1‑224 (MQ=255)
                                                                                                                         cATTTTGAAAACCGTGATAAGAACTGCTCTTACGGCTACCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                <  2:263292/238‑1 (MQ=255)
                                                                                                                          atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTCCCACTGCCCCTGAAGTTACTGACAGAAAACGGCTCACACCCGTTTTATTTCTCTCCGCCCTCCTGGTTTTTCCAAGGGGGATTTGTTTCTTTGAGCTTTTTTGCCCCGCCCGCGCAAAAATATTTACTTTTAATTCAATTTTTTAACTACa                                                                                               >  1:251274/1‑238 (MQ=255)
                                                                                                                            atTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTAATGCACAGTAAGTCTCTGACAGAAATAGGCTAACACCCGTTTGATTGCCCGACGCCCTCTGGGTTTTCCGATGGGGGATTTGTTTTTCTGTCCTTCTTGTCCCCGCCCGTACAAATATTTTTACTTTAAATTTCATTTGTTAACAACATc                                                                                             >  1:1138901/1‑238 (MQ=255)
                                                                                                                                  aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                                      <  1:1225070/239‑1 (MQ=255)
                                                                                                                                  aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCCCTGCACAGTAAGACTCTGACAGAAAAAGGCTAACACCGGTGTATTTTCCCCGCCCCCCCTTGGTTTTTCCCAGGCGGGTTTTTTTTTTTTTTCTTCTTTCCCCCCCCCCCCAAAAATATTTTTCTTTTaatttaattttttaataaaaaccctcaa                                                                                       >  2:1225070/1‑220 (MQ=255)
                                                                                                                                    ccGTTATAAGAACTGCTCTTACGACTACCTGCTGCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                    <  1:144019/239‑1 (MQ=255)
                                                                                                                                       tGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCAATGCACAGTAAATCTCTGACAGAAAACGGCCAACACCGGGTTTATTGCCCGACCCCCCCTTGGTTTTTCGCGGGCGGATTTGTTTTTATTTTCCTCTTTCCCCCCCCCCTCAAAAAATTTTTTCTTTTAATTTAATTTTATaaataaattcctttaccccc                                                                                  >  1:1323562/1‑226 (MQ=255)
                                                                                                                                         aTAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGAc                                                                                                                                                                                                     <  1:313549/128‑1 (MQ=255)
                                                                                                                                         aTAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                      <  1:1051739/216‑1 (MQ=255)
                                                                                                                                         aTAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                <  1:244579/222‑1 (MQ=255)
                                                                                                                                         aTAAGAACTGCGCTTACGACTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                    <  1:879183/234‑1 (MQ=255)
                                                                                                                                               aCTGCTCCTGCTACTACCTGCTGCACCCGGCAAGTTCTATTTCTAAAATCATTGGCGGTTGCCGTCCTACTGCACAGTAAGTCTCTGACAGAAACCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                                    <  1:99542/212‑1 (MQ=255)
                                                                                                                                                   ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTCCCCCGGAAGTATTTTATCAAAATCGGCCAAACCCCGCTTTATTTCCCGCCGCCCCCTCGGCTTTTTCAGGGCGTGTTTGGTTTTATGTCCTTTTTTTACCCCCCCTCCATAATATTTTTACTTTTAATTCTATTTTTTTACTACATCCCTTCACcacacacaccgccccc                                                                      >  2:879183/1‑226 (MQ=255)
                                                                                                                                                   ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGGGGGCCCCCTCCCCCCTAAATTTCTTTCAAAAATATGCTAACACCCGGTTTATTTCCCCCCCCCCCCCGGTCTTTTCGAGGGCGGGTTTTTTTTTTTTTTCTTTTTTTCCCCCCCCCCCAAAAAAATTTTTATTTTTATTTAATTTTTTAACTACAccccccttctcttttataaaactta                                                                      >  2:1051739/1‑216 (MQ=255)
                                                                                                                                                   ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGCTTCGCTCCCCCTCGCCATTAAGTTTCTTACAGAAAACGACTCACACCGCGTTAATTGTCCCCCACCCCCCCGAGTTTTCGAGGGCGTCGTTTTTTCTTTTTCCTTCTTCTCCCCCCCCCCCCAAAAATTTTTCCTTTTAATTTAATTTTTTAACTACCTccccttatcctctctcttataaaca                                                                     >  2:244579/1‑216 (MQ=255)
                                                                                                                                                    tctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCcccgc                                                                                                                                         <  1:328563/170‑1 (MQ=255)
                                                                                                                                                        aCGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACCCCCCAGGAAGATTCTGTAAAAAAACGGGTAAAACCGGGTTTATTTCCCCGCCCCCCCTCGGTTTTTCCGGGGCGGATTTTTTTTTTTTTCCTTTTTGCCCCCCCCCCCCAAAAAATTTTTCCTTTTAATTTCATTTTATAAATAAACCTCTTAACCACACcgcgccactctgcaaaa                                                                >  2:753481/1‑226 (MQ=255)
                                                                                                                                                              gccTGCCGCCACCGGCAATTTCTATGTCAAAAATCATTGGCGGTTGCGGGTCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt                                                          <  1:675723/238‑1 (MQ=255)
                                                                                                                                                                     gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGTCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                   <  1:2026/239‑1 (MQ=255)
                                                                                                                                                                      cACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACAGTAAGTCTCTTACAGAAAACGGCTAACACCCGGTTTATTTCCCCACCCCCCCTGGAGTTTTCCAGGGGGGATTTGTTTTTTTTTCCCTCTTTTCCCCCCCCCCAAAAAAAATTTTTCTTTTAATTTAAATTTATAACTACATTCATTTTCCCCACCGCCATCCCTTTCGGAAATTTGTGATCTtc                                                   >  1:264811/1‑238 (MQ=255)
                                                                                                                                                                       ccccGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                 <  1:620975/238‑1 (MQ=255)
                                                                                                                                                                       aCCCGGCAATTTCTATTTCCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                 <  1:790250/239‑1 (MQ=255)
                                                                                                                                                                          cGGCAATTTCTATTTTTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                              <  1:573017/239‑1 (MQ=255)
                                                                                                                                                                          cGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                              <  1:234096/239‑1 (MQ=255)
                                                                                                                                                                            gctatttttttttctAAAATCATTGGCGGTTTCGGTCCTACTGCACAGTAAGTCTTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                            <  2:982356/236‑1 (MQ=255)
                                                                                                                                                                            gCAATTTCTTTTTCTAAAATGATTGGCGGTTGCGGGCCTACTGCACATTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCTTATTTGTTTATATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                            <  2:276888/239‑1 (MQ=255)
                                                                                                                                                                            gCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGAc                                                                                                                                                                                                     <  1:309406/93‑1 (MQ=255)
                                                                                                                                                                            gCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                            <  1:753481/239‑1 (MQ=255)
                                                                                                                                                                            gCAATTGCTAGTTCTAAAATCATGGGCGGTTGCGGGCCTACCGCACAGTAAGTCTCGGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAGTTGATAACTACATCCATTAGCCACATCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                            <  1:584920/239‑1 (MQ=255)
                                                                                                                                                                             cAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGGCTCTGACAGAAATCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                            <  1:170024/238‑1 (MQ=255)
                                                                                                                                                                                 ttctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACa                                       <  1:638748/239‑1 (MQ=255)
                                                                                                                                                                                  tttttttctaAAATCTTTGGCGTTTGCGGTCCTACTGCCCAGTAAGTTTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                      <  2:322958/235‑1 (MQ=255)
                                                                                                                                                                                       ttcttAAATTCTTGGCGGTTTGCGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGTTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcat                                 <  2:661939/239‑1 (MQ=255)
                                                                                                                                                                                        tctaAAATCCTGTGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatc                                <  2:292916/239‑1 (MQ=255)
                                                                                                                                                                                           aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                             <  1:593032/239‑1 (MQ=255)
                                                                                                                                                                                            aaaatttttgtggttttctgttcTACTGACCAGTAAGTTTCTCAAAGAAATCGGCTAACACCGGTTTAATTTCCCGACGCCCTCGGGATTGTCCGAGGGCGTATTTGTTTAGATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                             <  2:181996/217‑1 (MQ=255)
                                                                                                                                                                                             aaTCATTGGCGGTTTGGGTCCTTCTGCCCAGTAAGTCTTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                           <  2:117935/239‑1 (MQ=255)
                                                                                                                                                                                                    ggCGGTTGCGGTCCTACTCCCCAGTAAGTCTCGGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtat                    <  2:1323562/239‑1 (MQ=255)
                                                                                                                                                                                                                tCCTACTGGCCAGTAAGTCCTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATg          <  2:106786/238‑1 (MQ=255)
                                                                                                                                                                                                                       gCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTTTATTTATCCTGCAtttgca  >  1:659157/1‑235 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                              
TTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCA  >  NC_000913/3499391‑3499843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: