| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,551,915 | A→C | 15.7% | V186G (GTG→GGG) | malP ← | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,551,915 | 0 | A | C | 15.7% | 136.1 / 2.9 | 73 | V186G (GTG→GGG) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (39/19); new base C (4/9); total (43/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCAGTATGGTTGTCGTTTGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGG > NC_000913/3551681‑3552148 | ggttttttttttttgTTTGGATAGA‑AACTTTGGCGCAGTTTTCCCCATTTGACCCCCTCTTGTCCGGCCCGCAAAAAATCCCCGTCGTTAAATTTAGTCAGATCAACGGGCGGCGCGTGGGG‑CGCCTG‑CCACAGCCCCGGCGCCTGCC‑CCCGCCCATTACGCTAGCCGCCAACGGGGAGAC‑CCCCCGTTTGACCGGTAATGGTAACCTCCGGCTCCCAGCGTCCGTCTTTCGTCACttt < 2:35991/225‑1 (MQ=255) gTCGTTTGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGCCAACGGGGGGAT‑CCCACGCTTGACCGGGAATGGTAAACTCCGGCTCCCAGGGTCCGCCTTTCGTACCTTTACCGCCAATc > 1:382985/1‑238 (MQ=255) gTCGTTTGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGCCAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCCCCCGGACTCCGTCTTTCTTCACTTTACCCCCAATc > 2:774321/1‑238 (MQ=255) gTCGTTTGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACAGTAGCCGACAACGGGGGGAT‑ACCACGCTTGGCCGGTAATGGTAAACTCCGCTTCCCAACGGCCGCCTTTTGCACCTTTACCGCCatac > 1:1250998/1‑235 (MQ=255) tCGTTTGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGCCCGTCTTTCGTCACTTTACCGCCAATc > 1:842872/1‑237 (MQ=255) tGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCACCGTCCGTCTTTCGTCACTTTACCGCCATTCCCTAcc > 1:1190659/1‑238 (MQ=255) gATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCAAAGACGCAGCGGCTGCG‑CAACGCCATTACGATAGCCGACAACGGGTAGAT‑CCCACGCTTGACCGGCAATGGTAAACTCCGGCTCCCAGCGTCCGCCTTTCGTCACTTTACTCCCTATCCCTACCGGc > 1:1141934/1‑239 (MQ=255) agaACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTCCCGCCAATCCCTACCTGCACAt > 1:316620/1‑238 (MQ=255) agaACTTTGG‑TCAGATTATCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGAAAGCCGACAACGGGCAACT‑CCCACGCTTGACCGCTAATGGGAAACTCCGGCCCCCAGCGACCGGCTTTCGACACTTTCCACACAACCCCTACCCGcccacc > 2:1303238/1‑237 (MQ=255) tttGG‑TGAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCCCGCCATTACAATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTATTGGAGAACGCCGGCTCCCAGCGGCCGTCTTTCGGCACTTTACCGCCAACCCCTACCTGcacacacacgg > 2:420057/1‑232 (MQ=255) tttGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTCCCGCCAATCCCTACCTGCACCTCCAgcg > 1:1031491/1‑237 (MQ=255) tttGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTCCACCTCCAGt > 1:243781/1‑238 (MQ=255) tttGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAAACCCGCCTCCCAGCGCCCGTCTTTCGGCCCTTTACCCCCAAAacctacctcccaaccaaag > 2:132968/1‑227 (MQ=255) tttGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGGAATGGGAAACTCCGGCTCCCACCGTCCGCCTTTCTCCCCTTTACCCCCAAACCCTACCTGCACACCCAgg > 2:914374/1‑237 (MQ=255) ttttCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTtgtg > 1:504380/1‑239 (MQ=255) tgtGCCGTGTTGTCCGGCACGAAAAAACCCCCCGTGTTAAAATTGGGTCATACCAAGCGGAGGCGGGTGC‑T‑CCCCGGCCCACACCCGGAGGGGCTGCG‑CCACGCCATTACGATAGCCGACAGCGGGAAGTT‑CCCACGTTTGACCGTTAATGGTAAACCCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACg < 2:946063/237‑1 (MQ=255) ttgttgtccggaccccAAAAAATCCCCGTCTTTAAATTTGTCCAGAGAAAACGGATG‑GCGGGGGTGCGCTGG‑CCCAAGGCGCAGCGGCGCCG‑CCACGCCATTACGAACGCCGACAACGGGGAGAC‑CCCACGCTTGACCGGTAATGGTAACCTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGt < 2:1080478/224‑1 (MQ=255) ctgTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCT‑‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGCCACTTTACCGCCAATCCCTACCTGCCCATCCAGGGCTTCGTTGTGGCGGAAACACGGGGAGtt > 1:254809/1‑236 (MQ=255) ttCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACCACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTCCACATCCAGGGCTTCGTTGTGTCGGAACCACgggagcgtaacg > 2:84545/1‑229 (MQ=255) cACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCCCATCCAGTGCTTCGTTTTGGCGGAACCACCGGTAGTTACTGCGATGc > 1:87422/1‑239 (MQ=255) cgtcgtTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCgcgcct > 1:339001/1‑234 (MQ=255) cgtTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTa > 1:878642/1‑212 (MQ=255) gtTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGGGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCa > 1:366131/1‑238 (MQ=255) cGGATGCGCGTGCGT‑CGCCCG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACaaaaa > 1:599125/1‑238 (MQ=255) gccccTG‑CCACAGACGAAGCGGGTGCG‑CATCCCCATTACGAAAGCCGACAAGGGGAAACT‑CCCACGCTTGCCCGTTAATGGTAACCTCCGGCTCCCGGCTTCCGTCTTTCGTCGCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACaa < 1:84545/235‑1 (MQ=255) tccccgcccccacccggtgCGGCTCCG‑CCACCCGATTCCGAACGCGCACAAGGGGAAGTC‑CCCCAGCTGACCCGGTATGGGAAAACCCCGTCTCCCAGCGTCCTTCTTTCGTCCCTTTCCCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACaa < 2:964904/220‑1 (MQ=255) cggcacaagacccgcgggcggcC‑CCCCGCCATTCCGATACCCAAAAAGGGGGAGAT‑CCACGGTTTGACCGGTAAGGGTAAACTCCGGCTCCCAGCGTCCGTCTTGCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCat < 1:901669/223‑1 (MQ=255) ccACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCCATGCCAGTCATCCGCCGCTTCCACCCGTTTCCCCTCACCaaaaaaacgcgggaaaaaaaccaatt > 2:733942/1‑217 (MQ=255) ccaaagcgccagggcgtcgcccacgcaaTTACGATAGCCGAAAAGGGGGAAAT‑CCCACGCTTGACCGGTAATGGAAAACCCCGGCCCCCAGCGTCCGTTTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCat < 1:1046998/216‑1 (MQ=255) accgccgcgggggCTGGC‑CCACGCCATTCCGATAGCCGACAACGGGGAGAC‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGa < 1:914374/228‑1 (MQ=255) aaccgcgaGCGGCCGCGCCCCCGCCATTACAATAGCCGAAACGGGGGAGAC‑CCCCCTGTTGACCGGGAAGGGTAAACCCCGGCTCACAGCTCCCGTTTTTCGTCCCTTCACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGa < 2:68258/232‑1 (MQ=255) gcgccgcgcgcg‑cCCCGCCTTTCCGATACCCGACACGGGGGATCT‑CCCCCGCTTTACCGGTAATGGTAAACCCCGCCCCCCAGCGTCTGTCTTCCGTCATTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTc < 2:339001/230‑1 (MQ=255) gaGCGGCTGCC‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTGCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCa < 1:804773/238‑1 (MQ=255) cacggggtccccccccccatcgcGAGAGCCGA‑AACGGGGGGGTCCCCACGTTTTACCGGTATGGGTAAACCCCGCCTCCCAGCGTCCTTTTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCa < 2:1190659/217‑1 (MQ=255) aGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAg > 1:68258/1‑239 (MQ=255) aGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCACGCGCTTCAACCCGTTTGCACTACAAAAAAAACCGGGGGAACAAAACATATTGaaaatt > 2:1046998/1‑231 (MQ=255) cg‑ccacgccaTTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGACCCCCGGGTAATTACTGCGATGCCAGTCATCCGGGGCTTCAACCTTTTTGCCATCGACAAAAGACTCGCGGAACAAACCCTAATTAAAGTTCAAAcccgaa > 2:993866/1‑234 (MQ=255) ggcccccgtggtgcGAGAGCCGACAAGGGGGAGCA‑CCCCCTCGTCGCCGTGAAGGACAATCCCCGGCTCCCCGCGTCCGTCTTTCGTCACGTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAAc < 2:101801/226‑1 (MQ=255) ccccccccttttccGATAGCCCACAGCGGGGAGCC‑CCCCCGCTTGCCCGGTAATGGTAAACCCCGGCCCCCAGCGTCCGTCTTCCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAAc < 2:599125/226‑1 (MQ=255) ccccccccaTTCCGAGAGCCGAAAACGGGGACTT‑CCCCGGCTTGCCCGTTAATGGAAAACTCCGGCCCCCGGGGCCCTTCTTCCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTGCTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAAc < 1:1106233/232‑1 (MQ=255) gccaTTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCAt < 1:1057032/185‑1 (MQ=255) gccaTTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCAt > 2:1057032/1‑185 (MQ=255) gtACGATAGCCGACACGGGGGAAAC‑CCCCCGCTTGCCCGGTAATGGTAAACTCCGCCTCCCAGCCTCCTTTTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGa < 1:774321/238‑1 (MQ=255) cagttgCCAACACCGGGGGGTC‑CCCACGGTTGTCCAGTAATGGTAACCCCCGTCCCCCGGCGTCCGTCTTCCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTTATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGATTg < 2:87422/234‑1 (MQ=255) tAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATc > 2:181825/1‑101 (MQ=255) tAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATc < 1:181825/101‑1 (MQ=255) tacccggacggggggaaccccccCGGTTGACCGTTAGCTGTAACCCCCGGCCCCCTGCCTCCGTTTTCGGTCACTTTCCCGCCAATCCCTACCTGCACAGCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACGGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGAc < 2:254809/219‑1 (MQ=255) gCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCCCAGACTGACCGa > 1:537482/1‑239 (MQ=255) gCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCAAATTGTTAATTCAAACCGTAACCCGTCGCAGaccgaccg > 1:841655/1‑238 (MQ=255) gaagcGGGGAATT‑CCCTCCCTTGAGCGGTAATGGTAACCCCCGGCCCCCAGCGTCCGTTTTTCGTCACTTTACCGCCAATCCCTACCTGCTCATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGt < 1:532278/235‑1 (MQ=255) ggAGAT‑CCCCCGTTTCCCCGGTAAGGTTAAACCCCGCCCCCCCGTCTCCGTTTTCCGTCCTTTCACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCAt < 2:949179/238‑1 (MQ=255) ggAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTTATAGTTCAGACCGTAACCCCTCGCAAACTGACCGCCAGTTTCCAtt > 1:911916/1‑239 (MQ=255) aaggtgaaaaagagacgggTAAACCCCGGCCCCCGAGGCCCGTTTTCCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGa < 1:271828/221‑1 (MQ=255) aCGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCAAATTGATAGTTCAGACCGTAACCCGCCGCAGACGACCCGCCAATTGCCCtttggacgcgg > 1:361331/1‑230 (MQ=255) cGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCAAATTGATAGTTCAGAACGGAACCCGTCGCAGACTGAACGACAGGTGCCATTGAGTCGAGGa > 1:554220/1‑239 (MQ=255) cTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGTCGGAACAAACCATATTGATAGTTCATACCGTCACCCGCCGCCAACTGACCGCCAATTGCCCTTTGGTCCAGGAAg > 1:488807/1‑239 (MQ=255) gACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCCCTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGa < 1:1280008/113‑1 (MQ=255) gACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGa > 2:1280008/1‑113 (MQ=255) gACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCCTTGAGTCGAGGAAGCa > 1:66393/1‑238 (MQ=255) aCCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACCAAAGACTGGCGGAACAAACCCTATTGATAGTTCATACCGTAACCCGTCTCACGCTGACCGCCCGTTTCCAtttgattcgtggagcca > 1:591752/1‑223 (MQ=255) gTAATGGAAAACTCCGGCTCCCAGCGGCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCCCGGGTAGTTACTGCGATTCCAGTCATCCGGCGCTTCAACCTGTTTGCCCACGTCAAAAGCATGGCGGAACAAACCCAATTAGTAATTACGACCGTAACCCGTGCCAGACTGAACGACAGTTGACATTTAGTCggaggagaacggccc > 2:905260/1‑225 (MQ=255) tGGTAAACCCCGGCTCCCGGCTCACGTTTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGa < 1:757526/239‑1 (MQ=255) gtaaccccgggCTCCCGGTGTCCGTCTTACGTCCCTGTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACg < 1:1303238/230‑1 (MQ=255) cgccTCCCTGCTCCCGTTTTTCGTGTCTTTCCCCCCAACCCCTACCCGCTCATCCAGGGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGac < 2:504380/236‑1 (MQ=255) cGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTAcc > 1:249857/1‑46 (MQ=255) cGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAAACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACGGACCGACCAATGCCATTTAGTCCAGGAAGCACGGCGCCAGAACACCCAGac > 1:339051/1‑239 (MQ=255) cGGCCCCCAGGCCCCGTCTTTCCTCCCTTGTCCGCCAATCCCTTCCCGCCCATCCCGTGCTTCGTTGTTGCCGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGac < 2:366131/239‑1 (MQ=255) cGGATCCCAGGGTCCGTCTTCCGCCCTTTTACCGCCAATCCCTGCCTGCACAGCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGac < 1:508475/239‑1 (MQ=255) ggCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTTACCGACAGTTGCCATTGAGTCACGGAAGCAAGCCGCCAGACGTCCCAGac > 1:1078440/1‑238 (MQ=255) gCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACCAAAGACTGGCGGAACAAAACATATTGATAGTTTCGACCGTAACCCGTCGCAGCGTGACCCCAAGTTGCCCTTTGGTCGAGGAAGCACCCCGCCAGagcacccccacca > 2:793399/1‑227 (MQ=255) tcTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCCTATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCCAACGTCCCAAACCACCGTTACCCAgcg > 1:637231/1‑238 (MQ=255) tttccGTCCCTTTACCGCCAATCCCTGCCGGTACAACCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAgcgc < 1:1275855/234‑1 (MQ=255) tCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGCCCGACAGTTGCCATTTAGTCGAGGAAGAACGCCGCCAGACGTCCCACACCACCGTTACCCCGCGCCggg > 1:1360183/1‑239 (MQ=255) | GCAGTATGGTTGTCGTTTGGATAGAGAACTTTGG‑TCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGT‑CGCCTG‑CCACAGACGCAGCGGCTGCG‑CCACGCCATTACGATAGCCGACAACGGGGAGAT‑CCCACGCTTGACCGGTAATGGTAAACTCCGGCTCCCAGCGTCCGTCTTTCGTCACTTTACCGCCAATCCCTACCTGCACATCCAGTGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGG > NC_000913/3551681‑3552148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |