Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,555,917 T→C 9.3% T311A (ACC→GCC)  rtcA ← RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,555,9170TC 9.3% 224.8 / 3.2 79T311A (ACC→GCC) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (39/31);  new base C (2/6);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCG  >  NC_000913/3555684‑3556153
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             
aaacacaatatcgcaaaTCGGTGTCAGAAAACCGGGGTGGGCCATTGCAAGGATGGGGTAAAACTCGCCCAGGAATTGGAGAAGATGGTGGGGTACGGGGTGTAAGTTTAGCCGGATAACGGGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTGACCCGCGGTACGGCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTcagca                                                                                                                                                                                                                                          <  2:1049299/224‑1 (MQ=255)
aaaaCGCATTACCGCAATCTTTATCAGAAACTCGGGGTGGCCCATCGCCAGGATGCGGGACAACACGGCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTcagca                                                                                                                                                                                                                                          <  2:610547/239‑1 (MQ=255)
  aaCGAATATCCGCAATCGGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGa                                                                                                                                                                                                                                        <  1:910213/239‑1 (MQ=255)
   aCGCATATCCGCAATCTGGATCAGAAACCCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGAt                                                                                                                                                                                                                                       <  1:612183/239‑1 (MQ=255)
      cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTAAATCTCTGCATCATTCAATGCTTACCCGCGTTACGCCATCTGTTTCTATCAAACTAAAACGCACCGGCAAGAAACGCCCCCACACCCCGGAATTGGTCAGCCAATgg                                                                                                                                                                                                                                     >  1:90761/1‑238 (MQ=255)
        tagccGCAATCTGTATCAGAAACTCGGCGGGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCAt                                                                                                                                                                                                                                  <  1:311915/236‑1 (MQ=255)
         taccGCAACTTGTTACAGAAACTCGGGGGGGCCCATCGCCAGGATGGGGGACAACACGCCCAGCAATTGCTGAAGAGGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATg                                                                                                                                                                                                                                 <  2:1100047/237‑1 (MQ=255)
         atCCGCAATCTGTATCAGAAACTCGGGGTGGCCCAGCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAGCGCGCCAGATCCGGCTTACATCTCGGCATCATTCAATGCTCGCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATg                                                                                                                                                                                                                                 <  1:90356/239‑1 (MQ=255)
                  ttttttCAAAAACTCGGCGTGGCCCATTGCCGGGTAGGGGTACAACACGCCCAGCAAGTGCTGAAGATGATGGGGTACGGCGTGTAAGGTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATggg                                                                                                                                                                                                                        <  2:644035/234‑1 (MQ=255)
                  cTGTATCAGAAACTCGGCGTGGCCCATCGGCAGGATGCGGTACAACACGCCCAGCAATTGCTGGAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGTCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATggg                                                                                                                                                                                                                        <  1:1220199/239‑1 (MQ=255)
                    gTATCAGAAACTCGGCGTGGCCCCTCGCCAGGATGGGGTACAACACGACCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCg                                                                                                                                                                                                                     <  2:761172/239‑1 (MQ=255)
                    gTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTATCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCCCACCCGCGTTACGCCATCTGGTTCTATCAAACTAAACCGCACCGCCAAGAAACGCCCCCACACCGCGAAATTGGTCAGCAGATGGCATGAGGGAgggggg                                                                                                                                                                                                                      >  1:644035/1‑237 (MQ=255)
                       acAGAACCGCGGCGTGGCCCATCGCCAGGATGCGGTACACCACGCCCTGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGAcc                                                                                                                                                                                                                  <  1:300489/238‑1 (MQ=255)
                               tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCCGGAAACGCGCCAGATCCGGCTTTCATCTCTGGACCATTCAATGCTCAACCGCGTTACGCCCACTGTTTCTATCAAACTAAACCCCACCCGCCAGAAACACCCCCCCCCCCGGCCATTGGCCACAGGAAGGGATGAGGGAGGGGCGAcacaaaaat                                                                                                                                                                                                           >  2:460337/1‑230 (MQ=255)
                               tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGGTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCCCGATATTGGTCAGCAGATGGCATGAGGGGTGGGCGACCGTAAAtt                                                                                                                                                                                                           >  1:775727/1‑238 (MQ=255)
                                 ggggTGGCCCGTCGCCAGGATGGGGTACAACGCGCCCAGCAATTGCTGAAGATGTTGGGTTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTccc                                                                                                                                                                                                        <  2:90761/236‑1 (MQ=255)
                                                  ggATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCAACCCGATATTGGTCAGCAGATGGCATGAGGGATTGGCGACCGTAAATTCCCCCGcgcccccccccgc                                                                                                                                                                                        >  1:1048052/1‑233 (MQ=255)
                                                               cacGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTTTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGGTATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGGAAATTCCCCCCCCCCCGCCACCGCCACCGGAAggcccc                                                                                                                                                                          >  1:1205198/1‑234 (MQ=255)
                                                                    ccAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTgg                                                                                                                                                                     >  1:217310/1‑239 (MQ=255)
                                                                     cagccttttgttgagattgtgGGGTTCGGGGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCCCCCGCGTTACGCCATCTGTTTCTATCAAACTAACCCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                                                                                                                                                    <  2:775727/220‑1 (MQ=255)
                                                                     cAGCAATTGGTGAAGGTGGTGGGGAACGGCGTGAAAGTTTAGCCGGATAACGGCCCAGATCCGGCTTAACTCTCTGCATCATTCAATGCTCCCCCGCGTTACGCCATCTTTTTCTATCAAACTAACCCGAACCGGCAAGAAACGCTCCACCCCCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                                                                                                                                                    <  2:1049442/239‑1 (MQ=255)
                                                                     cAGCAATTGCTGAAGGTGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCCCTGCATCATTCAATGCTCCCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                                                                                                                                                    <  1:348997/239‑1 (MQ=255)
                                                                     cAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                                                                                                                                                    <  1:584611/239‑1 (MQ=255)
                                                                         aaTTGCGGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGc                                                                                                                                                                <  1:1066208/239‑1 (MQ=255)
                                                                                  aggtgatgGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATAtt                                                                                                                                                        <  1:113846/235‑1 (MQ=255)
                                                                                   gatgatGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGc                                                                                                                                                                              <  1:1148984/215‑1 (MQ=255)
                                                                                             tACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCCGTTGGTCAGCAAGATATTCCCCCAccgcc                                                                                                                                             >  1:1030998/1‑238 (MQ=255)
                                                                                                ggCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCCCCACCACCCCGATATTGGTCAGCCGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCCTCCGTAGAACCAGTTGGGCAGCAAAATATCcccccccccccccc                                                                                                                                          >  2:1164752/1‑238 (MQ=255)
                                                                                                  cGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGGTACGCCATCTGTTTCTATCCAACTAAACCGCACCGGCAAAAAACGCTCCACCACCCCGGTATTGGTCAACCGATGGCCTAAGGGGTGGGGGGCCCGAAATTCCCCCCCGCCCCCCCACCCCCCCGGGAGCACCAGTTGGTCACCCAAATATTCCCCCCcccccgcccg                                                                                                                                        >  2:86912/1‑236 (MQ=255)
                                                                                                    tgtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACCTCTCTGCATCATTCAATGCTCACCCGCGTTACCCCATCTGTTTCTATCAAACTAAACCCCACCGGCCAGAAACGCCCCCCCCCCCCGGTATTGGTCAACAGATGGCATGAGGGATGGGCGGCCGTACATTCCCCCGCCCCCGCCAGCGCCCACGGGAGCACCCATTGGTCAGCAAGATATTCCCCCCCCGCCCCCGTCCt                                                                                                                                     >  2:991981/1‑239 (MQ=255)
                                                                                                    tgtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGAAGGGCGACCGTAAATTCCCCCGCGCCCCCCAGCGCCATCGGTAGCACCAGTTGGGCAGCAAGAAATTCCCCCCccccccccgg                                                                                                                                        >  2:858128/1‑235 (MQ=255)
                                                                                                    tgtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCCCCCCCACCCCGAAATTGGTCAGCCGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCCCCCGCCAGCCCCCCCCCAAGCCCCCGTTGGGCAGCAAAAAATTCCCCCAcccccccccg                                                                                                                                        >  2:331625/1‑234 (MQ=255)
                                                                                                            tAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGCGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCCCCGTGCTTGCCAggg                                                                                                                             >  1:90142/1‑238 (MQ=255)
                                                                                                              gCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCAt                                                                                                                                                                                                                                  >  2:1202136/1‑137 (MQ=255)
                                                                                                              gCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCAt                                                                                                                                                                                                                                  <  1:1202136/137‑1 (MQ=255)
                                                                                                              gCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGGACTTGCCAggaa                                                                                                                            >  1:483589/1‑236 (MQ=255)
                                                                                                                      cccccccagtcccGGTTTACATCTCGACATCATTCAAGCCTCCCCCGGTTTACCCAATCTGTTTCTATCAAACTAACCCGCACCGGAAAGAAACGCCCCCCCACCGCGATATTGGCCAGCAGAGGGCAGGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCAc                                                                                                                   <  2:1100400/228‑1 (MQ=255)
                                                                                                                       cgcgCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCa                                                                                                                    >  1:1100400/1‑237 (MQ=255)
                                                                                                                          ccagtgtCCGGTTTTCATTTCTCTCTCATTCAATGCTACCCCGGTTTCCGCCATCTGTTTCTAACAAACTAAACCGCACCGGAAAGAAACGCTCCCCCACCGCGATATTGGCCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACctct                                                                                                               <  2:590952/233‑1 (MQ=255)
                                                                                                                                    gCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCTCCGTGCTTGCCAGGCAGCGTTTCACCTCTTTCACCa                                                                                                        >  1:1134942/1‑236 (MQ=255)
                                                                                                                                      ttACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGAAGCGTTTCACCTCTTTCACCa                                                                                                        >  1:1306080/1‑234 (MQ=255)
                                                                                                                                        aCATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCACCAAGATATTCCCCCACCGCCCCCGGGCTTGCCCGGAAGCGTTTCACCGCTTTCACAAACtggg                                                                                                  >  2:386847/1‑236 (MQ=255)
                                                                                                                                         cATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGCCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGGGCTTGCCAGGCAGCGTTTCACCTCTTTCACCAAcc                                                                                                     >  1:590952/1‑233 (MQ=255)
                                                                                                                                              ctGCATCATTCAATGCCCCCCCGCGTTACGCCATCTGTTTCTATCAAACTAACCCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                                                                                           <  1:331625/239‑1 (MQ=255)
                                                                                                                                              acgcctttttcattccccccccGCGTTCCCCATCTTTTTTCTATCAAACCAACCCGCACCGGAAAGAACGGCCCCACCCCCGGAGTTTGGGCCAGCAGATGGCAGGGGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                                                                                           <  2:33497/220‑1 (MQ=255)
                                                                                                                                               tGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                                                                                           >  1:1014819/1‑238 (MQ=255)
                                                                                                                                                      ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                                                                                                                                                       <  1:71732/139‑1 (MQ=255)
                                                                                                                                                      ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                                                                                                                                                       >  2:71732/1‑139 (MQ=255)
                                                                                                                                                      ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTcc                                                                                                                                                      <  1:1281219/172‑1 (MQ=255)
                                                                                                                                                      ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTcc                                                                                                                                                      >  2:1281219/1‑172 (MQ=255)
                                                                                                                                                      ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTTCCAGGTAGCGTTTCACCTCTTTCCCCAACTGGGCCGCAACCCCCTCg                                                                                    >  1:1347462/1‑238 (MQ=255)
                                                                                                                                                           tGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCCCGGCAcc                                                                               >  1:33497/1‑237 (MQ=255)
                                                                                                                                                           tGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGCAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTg                                                                              >  1:667968/1‑239 (MQ=255)
                                                                                                                                                           tGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGAAGCGTTTCACCCCTTTCACCAACTGTGCCGCAACCCCCTCGGCCCt                                                                               >  1:1079745/1‑238 (MQ=255)
                                                                                                                                                             ccccccccctttcccccctCTTTTTCTAACAAACAAACCCGCACCGGAAAGAAACGTTCCCCCACCCGGATTTTTGTCAGCAAATGGCTTGAGGGATGGGCGACCGTAAATCCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc                                                                            <  2:1014819/221‑1 (MQ=255)
                                                                                                                                                                aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCa                                                                                                                                                                             >  1:1334195/1‑139 (MQ=255)
                                                                                                                                                                aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCAGCAACCACCTCGGCACTGCcgcg                                                                         >  1:948114/1‑239 (MQ=255)
                                                                                                                                                                       ttACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGCCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCACCCACCTCGGCACTGACGCGCcttcc                                                                   >  1:158711/1‑236 (MQ=255)
                                                                                                                                                                          cGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCTCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc                                                                >  1:4128/1‑239 (MQ=255)
                                                                                                                                                                            cccctttttttCTATCAAACCAAACCCACCCGGCAAAAAACGCCCCCCCCCCGCGTTTGTGGTCGACAGATGGCATGAGGGGTGGGGGCCCGAAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcga                                                              <  2:1205198/231‑1 (MQ=255)
                                                                                                                                                                                         ataaaaCTAAACCGCACCGGCAAGAAACGCTCCACCGCCGGGATATTGGTCAGGAGATGGCATGAGGGATGGGCGTCCGTAAATTCCCCCGCGCCCGCCAGCGCCTTCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCg                                                 <  1:414669/236‑1 (MQ=255)
                                                                                                                                                                                          tCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCg                                                 >  1:702668/1‑238 (MQ=255)
                                                                                                                                                                                           aaacctaccccccccccgCAAGAACCGCCCCCCCCCCTGGATATGGGCCAGCAGATGGCATGGGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGtt                                               <  2:1030998/222‑1 (MQ=255)
                                                                                                                                                                                              aCTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAACCGTTCgg                                            >  1:1262867/1‑239 (MQ=255)
                                                                                                                                                                                               cTAACCCGCCCCGGAAAAAAACGCCACCCCCCCGCGAGAGTGGTCGCCGGAGGGCATGGGGGTGGGGCGACCGGAAATGCCCCCCCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGt                                           <  2:483589/239‑1 (MQ=255)
                                                                                                                                                                                               cTAAACCGCACCGGAAAGAAACGCTCCACCCCCGGGATATTGGCCAGCAGATGGCAGGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGt                                           <  1:628929/239‑1 (MQ=255)
                                                                                                                                                                                                  aaCCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGCTGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCAGCCGTGCTTGCCAGGTAGCGTTTCACCCCTTCACCAACTGTACCGCAACCACCCCGGCACTTACGCGCTTTTCCCCCGCGCAAACAAAGCGTTCGGTGaaa                                       >  1:609728/1‑237 (MQ=255)
                                                                                                                                                                                                   aCCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCCCGGCCCTGACGCGCTTTTAACCGACGACAAAAAACCGTTCGGGGata                                       >  1:695169/1‑239 (MQ=255)
                                                                                                                                                                                                          cGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCCCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCACCTTCGCCCCAACCCCCTCGGCACTAACCCGCTTTTCACCCACCGCCACAAAGCATCCGTTGAACTTTTCaa                                >  2:295844/1‑238 (MQ=255)
                                                                                                                                                                                                                      cTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGCCGACAAAAAAGCGTTCGGGGACATTTTCACTTTCGCCTTCaa                    >  1:1349849/1‑239 (MQ=255)
                                                                                                                                                                                                                       cccccccccGCGATATCGGGCAGCAGATGGCATGAGGGATGGTCAACCGCAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAg                   <  1:452917/232‑1 (MQ=255)
                                                                                                                                                                                                                           cccccGCGATATTGGTCAGCAGATGGCATGAGGGATGGCCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgag               <  1:86912/236‑1 (MQ=255)
                                                                                                                                                                                                                            ccgcgcgATAGTGGTCAGCAGATGGCAGGGGGGGTGGGCGGCCGTAAATCCCCCCCCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgaga              <  1:991981/236‑1 (MQ=255)
                                                                                                                                                                                                                            cccgccattatTGGTCATGAGTTGGCATGAGGGATGGCCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgaga              <  1:460337/231‑1 (MQ=255)
                                                                                                                                                                                                                                 cgATTTTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTa         <  1:858128/239‑1 (MQ=255)
                                                                                                                                                                                                                                 cgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGGGACGGTa         >  1:573854/1‑239 (MQ=255)
                                                                                                                                                                                                                                      ttGGCCAGAGGATGGCATGGGGGAGGGGCACCCGTAAATTCCCCCCGGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc    <  1:766871/239‑1 (MQ=255)
                                                                                                                                                                                                                                         gTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGCCGACAAAAAAGCGTTCGGGGATATTTTCACTTTCGACTTCAACCGGACCGGAATAACCCg  >  2:98192/1‑238 (MQ=255)
                                                                                                                                                                                                                                         gTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGATACGGTATTACCCg  >  1:33400/1‑238 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             
AAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCG  >  NC_000913/3555684‑3556153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: