BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,784,899	A→C	15.9%	V39G (GTG→GGG)	yibN ←	putative rhodanese‑related sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,784,899	0	A	C	15.9%	284.4 / 7.5	117	V39G (GTG→GGG)	yibN	putative rhodanese‑related sulfurtransferase
Reads supporting (aligned to +/- strand): ref base A (58/36); new base C (2/20); total (60/57)
Fisher's exact test for biased strand distribution p-value = 6.97e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAA  >  NC_000913/3784667‑3785131

tttCGCGAGTGCGTTTGCGGGCTTCTGGCACTGACTGCAAGAACCGTTTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑GGGCTTTGATTTCGGTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCa                                                                                                                                                                                                                                       <  1:395733/239‑1 (MQ=255)

      tgttGCGTTTGCAGGCTCCTGGCACTTCATGCCAGAACCTTCTACCCATATAACCGGTTTTGC‑GTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCg                                                                                                                                                                                                                                 <  2:65011/236‑1 (MQ=255)

      cAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCg                                                                                                                                                                                                                                 <  1:664352/239‑1 (MQ=255)

           gttttGGAGGCTCCTGGCTCCTCACGCCAAAACCGTTTGCCATGATAACCGGTTTGTC‑CTTGGGGTTCTTAAGTCAAACAACATTG‑T‑TGGCTGTGATTTCGGTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTc                                                                                                                                                                                                                           <  2:654577/237‑1 (MQ=255)

            gTTTGCGGCCTCCTGGCCCTGCCTGCACAAACCGTTTACCCGGATAACCGTTTTGGC‑TTTGTGCTGCTCAAGCCCACCAACATTG‑T‑TGGCTTTAATTTCGCTCGGCAACAGAGTAATAGAA‑CCGGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCa                                                                                                                                                                                                                          <  2:1333444/239‑1 (MQ=255)

             tttgcctgttCCTGGCACTGAATGCCAGAACCGTTTCCCACGATAACCGGTGTGTT‑TTTGTGCTTCTCAAGCTCACAAACATTG‑T‑GTGCTTTGATTGCGCTCGGCAACAGATTAATAGAA‑CCTGCGAGATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAg                                                                                                                                                                                                                         <  2:447062/230‑1 (MQ=255)

             tttGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCa                                                                                                                                                                                                                          <  1:193867/238‑1 (MQ=255)

              ttGCAGGCTTCTGGCACTGCATGCCAGAACCGTCTACCCCGTTAACCGGTTTGTC‑TTTGTTCTTCTCAAGCTCCCCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAgg                                                                                                                                                                                                                        <  2:165113/239‑1 (MQ=255)

              ttGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAg                                                                                                                                                                                                                         >  1:986295/1‑238 (MQ=255)

              ttGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAg                                                                                                                                                                                                                         <  1:549769/238‑1 (MQ=255)

               tGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAgg                                                                                                                                                                                                                        <  1:994643/238‑1 (MQ=255)

                 gcggCTGCTGGCACTGCATGCCAGAACCGGTTACCACGATAACCGGTTTGTCGTTAGTG‑TTCTCAACGTCACCAACATTG‑T‑TTGGTTGTATTTCCGCCGGGAACCGATTAATTAAA‑CCTGCTATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAAGCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCTCCTTCACTTTCGA‑GGTCAGGctc                                                                                                                                                                                                                     <  2:897897/237‑1 (MQ=255)

                   ggCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCTA‑GGTCAGGCTCtt                                                                                                                                                                                                                   >  1:330770/1‑239 (MQ=255)

                   gcttCCTGTCATTGCCTTCCAAAACCGTTTACCACCATAACCGGTTTTTC‑TTTGTGCTTCTCAAGCTCACCAACCTTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCtt                                                                                                                                                                                                                   <  2:649762/236‑1 (MQ=255)

                      tCCTGGCACTGCATGACAGAACCTTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTaa                                                                                                                                                                                                                 <  1:559361/238‑1 (MQ=255)

                        cTGGCACTGCATGCCCAAACCCTTTTCCCCGTTAACCGTTTTGTC‑TTTGTGCTTTGTAAGCCCACCAACATTG‑T‑TGGCTTTGATTTCGCCGGGCAAAAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACg                                                                                                                                                                                                              <  2:425427/239‑1 (MQ=255)

                        cTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACg                                                                                                                                                                                                              <  1:900081/239‑1 (MQ=255)

                        cTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACg                                                                                                                                                                                                              >  1:1202029/1‑239 (MQ=255)

                          ggCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACgaa                                                                                                                                                                                                            >  1:1029435/1‑237 (MQ=255)

                                gCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCAA‑GGTCAGGCTCTTAAACTAAGTcaaa                                                                                                                                                                                                      >  2:118351/1‑237 (MQ=255)

                                 cATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCCCTTTCGG‑GGTCAGGCTCTTAAACGTAGACaaaa                                                                                                                                                                                                     >  2:1181622/1‑239 (MQ=255)

                                 cATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCCCTTAAACGGAGcaacaa                                                                                                                                                                                                     >  2:951892/1‑239 (MQ=255)

                                  aTGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCCCaaa                                                                                                                                                                                                    >  1:870772/1‑238 (MQ=255)

                                    gCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCCCCCCAAG‑T‑AAACACCTTCACTTTCGA‑GGTCAAGCCCTTAAActaatcaaaaaaa                                                                                                                                                                                                   >  2:107521/1‑226 (MQ=255)

                                      cAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTCGCTTCACCACCAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAACCGTCGTCACAAGAAc                                                                                                                                                                                                 >  1:1300904/1‑238 (MQ=255)

                                      cAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCCCTTAAACGAATTCCCAAAAAc                                                                                                                                                                                                 >  2:339383/1‑238 (MQ=255)

                                      cAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑CGTCAGGCCCTTAAACGTAGTCAAAAAAAc                                                                                                                                                                                                 >  2:1175981/1‑238 (MQ=255)

                                         acccGTCTACCACGATAACCGGTTTGTC‑TTTGGCCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGcc                                                                                                                                                                                             <  1:865379/237‑1 (MQ=255)

                                              tCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGC‑GGTCAGGCTCTTAAACATAGTCACAAAAACCGcccccac                                                                                                                                                                                        >  2:938295/1‑238 (MQ=255)

                                                   cACGATAACCGTTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTT‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCCCCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAAcg                                                                                                                                                                                   <  1:1181622/239‑1 (MQ=255)

                                                      ttgtcccctttttttttttGTGCTTCTCAAGCTCACCAA‑ATTGTT‑TGGCTTTGATTTCCCTCGGCAACAGATTAATAGAA‑CCTGCGATTGGGCCTTTACGGAA‑GTCAACACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGAACAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCCCTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAAcgcg                                                                                                                                                                                 <  2:768074/223‑1 (MQ=255)

                                                        tAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCCA‑GGTCAGGCTCTTAAACGTAGTCCCAAAAACCGCCACCAGTAACGCGacc                                                                                                                                                                              >  2:287389/1‑237 (MQ=255)

                                                            cGGTTTGTT‑TTTGGGCTTCCCAAGCCCCACAACCTTT‑G‑TGGATTTTATTTCGCTCGGCAAAAGATTAAAAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCCCTTTCGA‑GGTCAGGCTCTTAAACGTAGCCACAAGAACCGCCACCAGTAACGCGATCCAgg                                                                                                                                                                          <  1:446955/239‑1 (MQ=255)

                                                            cGGTTTGTC‑TTTGTGCTTCCCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCCCGGCAACAGATTAATAGAC‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAACCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAgg                                                                                                                                                                          <  1:1175981/239‑1 (MQ=255)

                                                               tttttttttttttctcttaaaGTTCCCCAATTTTG‑T‑GGGTTTTTGTTTCGCTCGACAACAGATTAATAGAA‑CCTGCTATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCTTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCg                                                                                                                                                                        <  2:1202029/220‑1 (MQ=255)

                                                               ttttttttttttgttttacAAACTCACCCAAATTT‑T‑GGTCTTTGATTTCCCACGGCAAAAGATTAAAAAAA‑CCGGCAGTATGGGCCTTACGGAA‑GGCATCACGGTGACTGAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCCCCACGAG‑T‑ACACCCTGTCCCTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCg                                                                                                                                                                        <  2:739483/221‑1 (MQ=255)

                                                                   tC‑TTTGTGCTTCTAAATCTCACCAACATTG‑T‑TGGCTTTGATTTCGCCCGGCAACAGATTAATAGAA‑CCTGCGATAGGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  1:951892/239‑1 (MQ=255)

                                                                   tC‑TTTGTGCTTCTAAAGCTCACAAAAATTG‑T‑GGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑TTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCCCCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  1:709038/239‑1 (MQ=255)

                                                                   tC‑TTTGTCCTCCTCAAGCCTACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GCCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGGGTGGCTTCACCACGAG‑T‑AATCACCTTCGCTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  1:361001/239‑1 (MQ=255)

                                                                   gtttttGTGTTTCCCAAGCCCCCCAACATTG‑T‑GGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCCGCAATAGGGCCTTTAGGGAA‑GTCATCACGCTTACGTAAATCCACAACCAC‑AGCGTCTTTTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATAc                                                                                                                                                                    <  1:664509/236‑1 (MQ=255)

                                                                    tttttggtcttccaaaGCTCCCCAACATGG‑T‑TGGCTTTGATTTCGCCCGGAAAAAGTTTAAAGGAC‑CCTGGGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  1:781763/225‑1 (MQ=255)

                                                                    tttttGTTCTTCTTCAGCTCCACACAATGG‑G‑TGGTTTTTATTTCCGCGGGCAAAAGATTAA‑AGAACCCGGCAAAATGGCCTTTAAGGAA‑TTACTCCCGGGAGCGTAAAT‑CACAACCCCAAGCGTCTTTTTTGTTGATCAGACGCGTAGCTTCCCCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCAAAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  2:1337014/237‑1 (MQ=255)

                                                                    tttttGTGCTTCCCAAGCCACCCACCTTTG‑T‑TGGTTTTTATTTCGCCCGGAAAAAAATTAAGAGAA‑CCCGCAATATGGCCTTTACGGAA‑GTCATCCCGCTGACGTAAATCCCCAACCAC‑AGCGTCTTCTTTGTGGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCCTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  2:527815/237‑1 (MQ=255)

                                                                       ttGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCCCAAGAACCGCCACCACTAACGCGATCCAGGCGATACTCAg                                                                                                                                                                >  1:558382/1‑239 (MQ=255)

                                                                       ttGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACAACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGAGGATatccag                                                                                                                                                                >  1:527138/1‑234 (MQ=255)

                                                                                         ccAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGCATGGGATGCCGGCcaac                                                                                                                                              >  1:386573/1‑239 (MQ=255)

                                                                                         ccAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGATTGGGATGACGGCcaac                                                                                                                                              >  1:1356851/1‑239 (MQ=255)

                                                                                             cATTG‑TGTGGCTTTTATTCCGCTCGGCAACATTTTAAAACAA‑CCTCAGATTGTGCCTTTGCGGAA‑GTAATCCCGGTGACTTAAATCAACACCCAC‑AGCTTCTTCTTTTGTGATCAGACGGGTAGTTTCACCACGAT‑T‑AATCCCCTTCCCTTTCGA‑GGTCAGGCTCTAAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACaaa                                                                                                                                           <  1:336532/239‑1 (MQ=255)

                                                                                                tg‑t‑tgGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGC‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCCCCAGTAAAGCGACCCAGGCGGAACACAGAATGGGGTAACGCCCaaaaaaaaga                                                                                                                                       >  2:1156331/1‑235 (MQ=255)

                                                                                                tg‑t‑tgGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCCGAACCGCGAGCCAGGCGTAACCCAGCATGGGATGACGACCAAAAAATTGc                                                                                                                                       >  2:480114/1‑239 (MQ=255)

                                                                                                   t‑tgGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGAAGGCCAAAAAAATgaaa                                                                                                                                     >  2:1058705/1‑236 (MQ=255)

                                                                                                         tttGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGAt                                                                                                                                                        >  1:173427/1‑215 (MQ=255)

                                                                                                               tttgcccGGCAACTGTTTTATTGAC‑CCGGCGTTGTGGCTTTTACGGAT‑GGTATCCCGGTGGCGTAAATCCACACCCAC‑AGCGTCTTCTTGGTTGAGCAGACGCGTAGCTCCACCACGAT‑T‑AATACCCTTCACTTTCGG‑GGCCAGGCTCTTAAACGTAGTCAAAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCa                                                                                                                          <  2:673872/233‑1 (MQ=255)

                                                                                                               ttcccccGGCAAAAGTTTAAAAAAA‑CCCGCAATTTGGCTTTTGCGGAA‑TCCACCCCGCTAGCTAAAATCCCCAACCAC‑GGCGTCTTCTTTGTTGATCAGACCGGTAGCTTCCCCACGAG‑T‑AACCACCTTCACTTTCGA‑GGCCAGGCTCTTAAACGTAGGCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCa                                                                                                                          <  2:1356851/233‑1 (MQ=255)

                                                                                                               ttccatggccaacaaATTAAAAGAC‑CCTGCAAGAGGGGCTTTCCGGAA‑GCCACCCCGTGGACATAAAACCCCAACCAC‑AGCGTTTTCTTTGTTGATCAGACGCGTAGCTTCACCAGGAG‑T‑AATCACCTTCCCTTTGGA‑GGTCAGGCTGTTAAACGTAGACAAAAGACCCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCa                                                                                                                          <  2:1167385/230‑1 (MQ=255)

                                                                                                               tccctccGGCAACCGATTAATGAAA‑CGTCCGATAGGGCCTTTACGGAA‑GTTAGCACGATGGATTAAATCCAAAACCCA‑CGCCTCTTTTTTGGTGATCAGACGCGTAGCTTCACCACGAA‑T‑AATCCCCTCCCCTTGCGA‑GGTCAGGCTTTTAAACGTGGTAAAAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCa                                                                                                                          <  2:1029435/233‑1 (MQ=255)

                                                                                                                 cGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCCAACCCGGCGATAATCAATATGGGAATCCGGCCAACAAATTGCAAAATTTCtagaagg                                                                                                                        >  2:721963/1‑233 (MQ=255)

                                                                                                                   cTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACt                                                                                                                                                                   <  1:967354/194‑1 (MQ=255)

                                                                                                                           acgtttAAAAGAA‑CCTGCGATTTGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTTTTCTTTGTTGATCAGACGCTTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTaaca                                                                                                             <  1:250561/235‑1 (MQ=255)

                                                                                                                                       a‑CCTGCGATTGGGCCTTT‑CGGAAGGTCACCACGCTGAAGAAAACCCCCAACCCG‑AGCGTCTCCTGTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGGCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTa                                                                                                 <  1:100786/239‑1 (MQ=255)

                                                                                                                                         ccTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGAAACTCAGTATGGGAGGACGGCCAACAAATTGCATAATTCCTTGCA‑TGGGGGGAAACAACTCCCAACGAAg                                                                                                >  1:1115441/1‑239 (MQ=255)

                                                                                                                                         ccTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCACCCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGCCCCACACATTGCATAATTTCTTGCC‑TGGGGGGTAACAACCCCCGACGCAg                                                                                                >  2:1287157/1‑239 (MQ=255)

                                                                                                                                            gCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGCATGGGa                                                                                                                                                         >  1:1125470/1‑180 (MQ=255)

                                                                                                                                                       tttACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGCCGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGAACTCACAAGAACCGCCACCAGTAACGCGATCCAGGCCATACTCAGCATCGCACGCCGCCCAACACA‑TGCCTAAATTCTTGCACTGGGGGGAAACAACACCCGAcagaatgacacaaaaaaa                                                                                   >  2:580926/1‑221 (MQ=255)

                                                                                                                                                       tttACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAAcc                                                                                 >  1:1136142/1‑239 (MQ=255)

                                                                                                                                                       tttACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGGCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGACCCAGGCGCAACCCCGTATGGGATGAAGGCCAACAACTTGGAGAATTTCTTGAA‑AGGGGGGACAAAATCCGCGACGTAAAGT‑Taaaaaaaaac                                                                                 >  2:50690/1‑237 (MQ=255)

                                                                                                                                                         tACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGAATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTGACAACTCCAGAGGAAGTGA‑TTAAAAAAAcctg                                                                               >  1:771145/1‑237 (MQ=255)

                                                                                                                                                           cGGAA‑GTCATCACGCTGACGTAAACCCACAACCAC‑AGCGTCTGCTTGGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTGCACTCTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGa                                                                             <  1:287389/239‑1 (MQ=255)

                                                                                                                                                             gaaagccccccggctacctaaaaTCCACACCCAC‑GGCTTCTTCTTTGTTGGTCAGACGCTTAGCTTCACCCCGAG‑A‑ACTCCCCTTCACTTTCGA‑GGTCAGGCTCTTAACCGGAGTCAAAAGACCCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGaa                                                                            <  2:1329145/219‑1 (MQ=255)

                                                                                                                                                             gAA‑GTCATCCGGCGGACGTAACCCCCAAACCAC‑AGCGTCTTCTGTGTTGAGCAGACGCGTAGCTTCACCACGAT‑T‑AATCACCTTCACTTGCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGaa                                                                            <  1:1248001/238‑1 (MQ=255)

                                                                                                                                                              gaatctacccccgagccaaaaaCCCACAACCCA‑GGCCTTTTATTTGTTGAACAAACGGTTGTCTTCCCCA‑GAGAT‑AACCCCCTTCAC‑TTCGAGGGTCAGGCTCTTAAACGTAGTCAAAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGaa                                                                            <  2:870772/219‑1 (MQ=255)

                                                                                                                                                               aagccaacccggggACGTAAAGCCAAAACCCA‑AGGGTTCTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AACCACCTTCACTTGGGA‑GGTCAGGCTCTTAAACGTAGTCAAAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGaa                                                                            <  1:1116435/224‑1 (MQ=255)

                                                                                                                                                               a‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCACCAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAAATCCCCACGAAGTGA‑TTAAAAAAACAAGGAAAgg                                                                         >  2:1175395/1‑239 (MQ=255)

                                                                                                                                                                  tcaccccgttGACGTAAATCCCAAACCAC‑AGCGTCTTCTTGGTTGATCAGACGCGTAGCTCACCCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAg                                                                       <  1:118351/230‑1 (MQ=255)

                                                                                                                                                                  tcaccccgcgtaCGTAAACCCACAACCCC‑AGCGTCTTCTTTGTTGATCAGGCGGTTAGCTTCCCCAGGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAg                                                                       <  1:1049293/228‑1 (MQ=255)

                                                                                                                                                                    atcaCGGTGAAGTAAACCCAAAACCAC‑AGCTTCTTCTTTGTTGATCAGGCGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGta                                                                     <  1:1058705/239‑1 (MQ=255)

                                                                                                                                                                    atcaCGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCCCCAGTAACGCGATCCAGGCGAAACTCAGTATGGGCTGACGGCCAACACATTGCTTAATTTCTTGAC‑TGGGGGGAACCAACTCCGGCCCGA‑TGATTTAAAAAAACCAGGAAAgggtaa                                                                     >  2:918449/1‑235 (MQ=255)

                                                                                                                                                                    atcaCGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGTTACTCAGGATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGAAGTGA‑TTAAAAAAACAAGGAAAGGAgaa                                                                     >  1:686190/1‑237 (MQ=255)

                                                                                                                                                                    atcaCGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGta                                                                     >  1:29095/1‑239 (MQ=255)

                                                                                                                                                                            gACGTAAACCCACAACCAC‑AGGTTCTTCTTGGTTGATCAGACGCGTAGCTTCACCACGAT‑T‑AATCACCTTCACTTTCGA‑GGTCAGCCTCTTAACCGTAGTCAAAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTgcg                                                             <  1:1364779/239‑1 (MQ=255)

                                                                                                                                                                                aaaaCCCCCAACCAA‑AGC‑TTTTTTTTTTTGGACAGACGGGTTACCTCACCCAGAG‑GAAATCCCCTTCACGTGGGA‑GGCCGGGCTCTAAAAAGTGTTCAAAAAAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGt                                                         <  2:827913/238‑1 (MQ=255)

                                                                                                                                                                                 taaacccccacccccgctgTCTTTGTTGTTGATCAGACGCGTAGCTTCCCCACGAG‑T‑AACCACCTTCATTTTCGA‑GGTCAGCTTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGt                                                         <  1:738473/221‑1 (MQ=255)

                                                                                                                                                                                 aactCCCCAACCAC‑AGCTTCTTTTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCCCCTTCACTTTCAA‑GGTCAGGCTCTTAAACGTGGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGt                                                         <  1:1156331/235‑1 (MQ=255)

                                                                                                                                                                                       acaACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGCG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTgcgagtgtcggcat                                                  >  1:1344971/1‑228 (MQ=255)

                                                                                                                                                                                       acaACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCaa                                                  >  1:168257/1‑239 (MQ=255)

                                                                                                                                                                                       acaACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACAAGGAAAGGAGAATACCTGCGCGGGGCGGCaa                                                  >  1:1038090/1‑239 (MQ=255)

                                                                                                                                                                                       acaACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGAAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCCGTGCGGCaa                                                  >  1:676585/1‑239 (MQ=255)

                                                                                                                                                                                         aaCCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGG‑TTAAACAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCaatt                                                >  1:56789/1‑237 (MQ=255)

                                                                                                                                                                                              aaaaagggactgtttgtgATCACACGCGTGACTTCACCCAGAG‑T‑AATCACCTTCCCTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATAcag                                            <  1:946808/223‑1 (MQ=255)

                                                                                                                                                                                                  cGTCTTCTTTGTTGATCAGACGCGTCGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCACGACGAAGTGA‑TTAAAAAAAACAGGAAAGGAGAATACCTGCGGGGGGCGGCAAAAAcaaccaa                                        >  2:1097908/1‑238 (MQ=255)

                                                                                                                                                                                                      ttcttTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGcgcg                                    >  1:1061501/1‑239 (MQ=255)

                                                                                                                                                                                                      ttcttTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATAAAGCAAGcgcg                                    >  1:1126279/1‑239 (MQ=255)

                                                                                                                                                                                                      ttcttTGTTGACCAGCAGCGTAGCTCCCCCACGAG‑A‑AACCCCCGTCACTTCCGG‑GGTCAGGCTCTTAAACGGAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGcgcg                                    <  1:402226/239‑1 (MQ=255)

                                                                                                                                                                                                       tcttTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATAcagc                                           >  1:291513/1‑231 (MQ=255)

                                                                                                                                                                                                                aTCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATa                                               >  1:401406/1‑218 (MQ=255)

                                                                                                                                                                                                                     aCGCGTAGCTTCACCACGCG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGCCGTAATGA‑TTAAAAAAACCAGGAAAGGAGGATACCTGCGCGGTCCGGCAAATCCAGCCAGAGCG‑TAACTgtcccttaaa                    >  2:946808/1‑230 (MQ=255)

                                                                                                                                                                                                                          tAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTg                >  1:88544/1‑239 (MQ=255)

                                                                                                                                                                                                                                caccacGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAAGGCAATTTTGCGGGgc          >  1:851588/1‑239 (MQ=255)

                                                                                                                                                                                                                                     cGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAAc                               >  1:364767/1‑213 (MQ=255)

                                                                                                                                                                                                                                       aG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGC‑TTAAAAAAACCAGGAAAGGAGAATACCGGCGCGGCGCGGCAAATAAATCCCGGGCGCTACCTGGAAACCAATTTT‑CGGGgggccgcgga  >  2:869981/1‑233 (MQ=255)

TTTCGTCAGTGCGTTTGCAGGCTCCTGGCACTGCATGCCAGAACCGTCTACCACGATAACCGGTTTGTC‑TTTGTGCTTCTCAAGCTCACCAACATTG‑T‑TGGCTTTGATTTCGCTCGGCAACAGATTAATAGAA‑CCTGCGATATGGCCTTTACGGAA‑GTCATCACGCTGACGTAAATCCACAACCAC‑AGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAG‑T‑AATCACCTTCACTTTCGA‑GGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCA‑TGGGGGGTAACAACTCCCGACGTAGTGA‑TTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAA  >  NC_000913/3784667‑3785131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`