Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,016,069 A→G 13.7% S34P (TCT→CCT)  ysgA ← putative carboxymethylenebutenolidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,016,0690AG13.7% 251.4 / 2.5 104S34P (TCT→CCT) ysgAputative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base A (72/13);  new base G (3/15);  total (76/28)
Fisher's exact test for biased strand distribution p-value = 4.76e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATC  >  NC_000913/4015833‑4016301
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          
tACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGCCCACTGGCCGTGGG‑CCAT‑CGCTTTGCTTTGGCCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAgggg                                                                                                                                                                                                                                          >  1:834619/1‑239 (MQ=255)
    aGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGCCCACTGGCAGTGGG‑CCAT‑CGCTTTTCTTTGGTCTGGCATGGTAACAAGGCATTTTATTCCCTTGTGAGGGGTAAAAGGT‑Gaa                                                                                                                                                                                                                                     >  2:45899/1‑239 (MQ=255)
    aGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCCGTGGG‑CCAT‑CGCTTTGCTTTGTGCTGGCATGGTAAGCAGGCATGTTATCCCCTGTTGAAGGGTAAGAGGG‑Gaa                                                                                                                                                                                                                                     >  2:927221/1‑239 (MQ=255)
      aCCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAACCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAg                                                                                                                                                                                                                                    >  2:449709/1‑238 (MQ=255)
      aCCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGCCTGGCATGGTAAGCAGGCATGTTATCCCCTTTGGAAGGGTTAAAGGG‑Gaaa                                                                                                                                                                                                                                    >  2:279138/1‑237 (MQ=255)
         gCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGccc                                                                                                                                                                                                                                 >  1:1097651/1‑238 (MQ=255)
                       ggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGAAAGGGGG‑AAAGCCCGCCCCAAAcgcgcc                                                                                                                                                                                                                   >  2:1058654/1‑236 (MQ=255)
                          tatCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTcc                                                                                                                                                                                                               >  1:1043045/1‑239 (MQ=255)
                            tCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGAAAGCAGGCCTGTTATCCCCTTGTGCAGGGGTCGGGGT‑GAAGCCCGCCACAATCGCGTCGTTCgg                                                                                                                                                                                                             >  1:1278142/1‑239 (MQ=255)
                             cGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCCCAATCGCGTCGTCCggg                                                                                                                                                                                                            >  1:214934/1‑239 (MQ=255)
                             cGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAG‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATGAAGGG‑GAAGCCCGCCACAATCGGGTCGTCCggg                                                                                                                                                                                                            >  2:401833/1‑239 (MQ=255)
                                        ttCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCCGGCATGTTATCCCCTTGTGACGGGATAGAGGT‑GAAGCCCGCCCAAATCACGTCGTCCGGGGCCTaaaagtg                                                                                                                                                                                                 >  2:1137793/1‑235 (MQ=255)
                                         tCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATACCCTTGTGAAGGGGTAAGGGT‑GAAGCCCGCCCCAACGCCGGCGTCCGGGGCCTgcagggtg                                                                                                                                                                                                >  2:1190525/1‑232 (MQ=255)
                                                           agtaaattcctggtgacgtggCCAGATCCCCCTCAAGCCTGAGAGGGCGACAAAGGACGCGTATATATGCATGCAACACAAAATTT‑C‑TCTG‑ACAGTAAAGACCCCGTGTGGGGGG‑CCCTGCGTTTTGGTGGGGTCGGGCAGGTTGAGCAGCCATGTTTCCTCCTGGGGAGGGGATGGGGGT‑GAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGAt                                                                                                                                                                             <  2:204315/219‑1 (MQ=255)
                                                                    cAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCGAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCCCAATCCCGTCGTCCGGGGGCGGAAAGATGGACGAAGAGAGA‑GGGGAGGCGGCAggg                                                                                                                                                                    >  2:536686/1‑238 (MQ=255)
                                                                    cAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGt                                                                                                                                                                    >  1:1199005/1‑239 (MQ=255)
                                                                       gTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGCCCGGGGTCTGAACGATGGTCGAACAGAGG‑GGGGCTGCCGCAggggga                                                                                                                                                                 >  2:949025/1‑235 (MQ=255)
                                                                            aTAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGACGGGCTAGAGGT‑GAAGCCCGCCACAATCGGGTCGTCCGGGGTCTGAACGATGGGCAGAACGAGA‑GGCGCTGACACGGGGGCAAATca                                                                                                                                                            >  2:932531/1‑238 (MQ=255)
                                                                            aTAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGAAGCAGGGGCAAATca                                                                                                                                                            >  1:264795/1‑238 (MQ=255)
                                                                            aTAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGG‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATcc                                                                                                                                                            >  1:231674/1‑239 (MQ=255)
                                                                                 cagaccccccccAAGCCCAAGACGGCAAAAAAGGGCGGGTATTTGTTCATGCAC‑CAAAAAATT‑TCCCGGCCCTAAAATGACCCCGGGGAGTGGG‑CCAT‑CGTTTTTGTGTGGTCGGGCATGGTAAGCAGGCATGTTACCCCCTTGGGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGa                                                                                                                                                         <  1:1251845/231‑1 (MQ=255)
                                                                                    aTACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGAttgtt                                                                                                                                                    >  1:1294198/1‑239 (MQ=255)
                                                                                          cTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCCCAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATtgttgggttg                                                                                                                                              >  1:841908/1‑239 (MQ=255)
                                                                                          cTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGC‑AGAGGGCGATGCAGCAGGTGCAAATCCAAATtgttgggttg                                                                                                                                              >  2:900547/1‑239 (MQ=255)
                                                                                          cTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGGCTGAACGATGGGCGAAGCGGGA‑GGCGATGCAGCAGGGGGAAATCCGAATtgtttggttg                                                                                                                                              >  2:1045536/1‑239 (MQ=255)
                                                                                          cTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGCCTGAACGATGGTCGCAGCGCAA‑GGCGATGCAGCAGGTGCAAACCCAGATtgttgtgtgg                                                                                                                                              >  1:213818/1‑237 (MQ=255)
                                                                                           tCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGGGAGA‑GGCGATGCAGCAGGGGCAAAACCAGATTgttgggttgt                                                                                                                                             >  2:172512/1‑239 (MQ=255)
                                                                                             cagcgcaagcgggcacaaaaTGCCGCGGTTTTGTTACGGCAC‑CCAAAAATT‑TCCCTGCACTACAATGCCCACTGGCGGTGGC‑CCAT‑CGTTTTGCTTGGGTCTGGCATGGTAAGCAGGCATGTTACCCCCTTGTGAGGGAATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGttgttg                                                                                                                                           <  1:1045536/222‑1 (MQ=255)
                                                                                              aGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGCCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTGGc                                                                                                                                          >  1:840353/1‑239 (MQ=255)
                                                                                                  ccAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGCCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATg                                                                                                                                      >  1:230949/1‑239 (MQ=255)
                                                                                                     gACGGCGACAAATGCCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGG‑AAAGCCCGCCACAATCGCGTCGTCCTGGGTCTGAACGATGGCCTAAGCGACA‑GGCGATTCAGCAGGCGGCAATACAGATGGGTGCGGTGTTGCCATGGTa                                                                                                                                   >  2:1229151/1‑239 (MQ=255)
                                                                                                       gggccaaaaaTGCCGGGGATTTGTTAATGCCCGCAAAAATTT‑T‑CCGGCACTACAATGACCTGTGGCAGTGGC‑CCAT‑CGCTTTGCTTGGGCCGGGCAGGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                                                                                                                  <  1:45899/231‑1 (MQ=255)
                                                                                                       ccgccaaaaaTGTCTCGGTTTTGTTCCTCCCCCACAAAAATT‑T‑CCCGTCCTTAAACTGCCCCGGGCAGTGGGCCCAT‑CGTTTCGTTGGGGTGGGGCATGGAAGGCGGGTGTTTTACCCCTTTGGAAAGGGATGGGGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                                                                                                                  <  2:290595/232‑1 (MQ=255)
                                                                                                                aTGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGGGTTGTTGCCATGGTATTCTCCGTAc                                                                                                                         >  1:857491/1‑238 (MQ=255)
                                                                                                                  aaaagtcaatttttttcgtcccccAAAAATT‑TCCCCGCACTAAAAGGCCCCCGGGGAGGGGG‑CATG‑CGTTTTGTTTGGGGTGGGAAGGGAAAGCAGTCTGTTTACCCCGTTGTGAGGGGATAGGGGT‑GAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGGCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTAccc                                                                                                                       <  2:530011/217‑1 (MQ=255)
                                                                                                                             gTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGCGA‑GGCGATGCAGCAGGGGCAAATCCGGATTGTTGCGTTGTTGCCATGGGATTCTCCGTACCCTTATAAAAATGt                                                                                                           >  1:1057893/1‑239 (MQ=255)
                                                                                                                              ttCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCCGGGGCAAATCCAGATTGTTGTGTTGTTGCACTGGACTTCCCCTGACCCTTTAAAAAAtttt                                                                                                          >  2:56002/1‑239 (MQ=255)
                                                                                                                                   gCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTACCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTCCGTACCCTTATAAAAATGTTGCGCa                                                                                                     >  1:907090/1‑239 (MQ=255)
                                                                                                                                   ccacccaaaaatTT‑T‑CCTCCACTAAAATGCCCACTGGCGGGGGC‑CCAT‑CGTTTTGTTTTGGTCTGGCATGGTAAGCAGGCATGTTACCCCTTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCa                                                                                                     <  1:927221/232‑1 (MQ=255)
                                                                                                                                     aCGCCAAAATTT‑T‑CCGGCCATACAATGACCACGGGCAGGGGG‑CCAT‑CGCTTTGCTTTGGTCGGGCAGGGTAAGCAGGCATTTTACCCCTTTGTGAAGGGATAGAGGG‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAt                                                                                                   <  1:401833/239‑1 (MQ=255)
                                                                                                                                      cGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGCTgtctcttt                                                                                                  >  1:530011/1‑232 (MQ=255)
                                                                                                                                      acccAAAAATTGT‑CCTGCACAAAAATGCCCCTGGGCAGTGGG‑CCCT‑CGCTTTGCTTTGGTCGGGCATGGTAAGCAGGCATTTTCCCCCCTTGGGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAt                                                                                                   <  1:172512/237‑1 (MQ=255)
                                                                                                                                       gCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGGGTTGTTGCCATGGTATTTCCCGTACCCTTTTAAAAATGTTTCGCAATGt                                                                                                 >  1:184963/1‑239 (MQ=255)
                                                                                                                                        ccAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGGGTTGTTGCCATGGTATTCTCCGTCCCCTTATAAAAATGTTGCGCAATGtt                                                                                                >  1:184181/1‑239 (MQ=255)
                                                                                                                                         cAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTa                                                                                               >  1:43210/1‑239 (MQ=255)
                                                                                                                                                  t‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCCCAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGCTGCATCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTCTAAAAATTGTGCGAAATTTTAACTATAGt                                                                                       >  1:1196469/1‑239 (MQ=255)
                                                                                                                                                           tACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGCGGT‑GAAGCCCGCCACAATCGCGCCGTCCGGGGTCTGAACGATGGGCGAAGCGAGC‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGGCATGGGATTCTCCGTACCCTTATAAAAATGTTGCGCAATTTTAACCATAGTCACCAGGc                                                                               >  2:708794/1‑239 (MQ=255)
                                                                                                                                                                 gcccaggggggggggg‑CCAG‑CGTTTTGCTTGGGTCGGGCAGGGTAAGCAGGCATTTTCCCCCTGTGGGAAGGGAAAGGGGG‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                                                          <  1:89776/225‑1 (MQ=255)
                                                                                                                                                                    cccgtgggaggtggcccttccttttgctttgGTCTGGCATGGTAACCAGTATTTTTACCCCTTTGTGAAGGGATAGAGGT‑AAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                                                          <  1:900547/215‑1 (MQ=255)
                                                                                                                                                                     aCTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc                                                                                             >  1:230170/1‑215 (MQ=255)
                                                                                                                                                                      cgggCAGGGGG‑CCAC‑CGTTTTGTTGTGGTCGGGCATGATAAGCAGGATAGTTATCCCCGTGGGAAGGGATAGAGGG‑GAAGCCCGGCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                                                    <  1:1020100/237‑1 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGGGATGCAGCGGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCACCAAATCACAt                                                                   >  1:118959/1‑239 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCCCAt                                                                   >  1:73231/1‑239 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCCCAt                                                                   >  1:1087710/1‑239 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAt                                                                   >  1:813888/1‑239 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCACCATGCAACAAATcaact                                                                   >  1:324593/1‑236 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGGGTTGTTGCCATGGCATTCTCCGTACCCTTTTAAAAATGTTGCGCAAGGTTAACTAAAGTCACCATGCAACAAATCACAt                                                                   >  1:305893/1‑239 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGAATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAt                                                                   >  1:613780/1‑239 (MQ=255)
                                                                                                                                                                       tGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGAGGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGAATTCTCCGAACCCTCATAAAAATTTTGGGCAAGTTTAACTATAGTCACCAGGAaaaaaaaccaaa                                                                   >  2:1020100/1‑233 (MQ=255)
                                                                                                                                                                        ggggctgtgcgcccgttgtttgtttggGTGTGGCTGGGAAAGAGGGCATTTCATCCCTGGGGGAAGGGATGGGGGT‑GAATCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                                                    <  2:923899/221‑1 (MQ=255)
                                                                                                                                                                        ggCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                                                                                                              >  1:659623/1‑179 (MQ=255)
                                                                                                                                                                            gTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTTTCCGTACCCTTATCAAAATGTTTCGCACGTTTAACTTTAGTCAGCATGCAACAAAAAACATTGc                                                                >  2:89776/1‑237 (MQ=255)
                                                                                                                                                                                  ccAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGCGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATCGTCAACATGAAACAAATAAAATTGCCTAAAtg                                                         >  2:679621/1‑238 (MQ=255)
                                                                                                                                                                                    aT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCg                                                        >  1:1265042/1‑238 (MQ=255)
                                                                                                                                                                                     ttctttttgttttgGTCTGGTGTGGAAAGCTGGTTTTTTCTCCTTTGGGGAAGGGAGGGGGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCgg                                                       <  2:230949/234‑1 (MQ=255)
                                                                                                                                                                                        gttttgctttgGTCGGGCATGGAAGGCAGGTATTTTACCCCTTTGTGAAGGGATAGGGGG‑GAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTc                                                    <  1:536686/237‑1 (MQ=255)
                                                                                                                                                                                           ttgctttgGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCa                                                   >  1:1194326/1‑237 (MQ=255)
                                                                                                                                                                                           ttgctttgGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCa                                                   >  1:712436/1‑237 (MQ=255)
                                                                                                                                                                                                  ttgtgggtggggtaggcgtgCATGTTGTCCCGTGGAGGGGGTATGGGGGG‑GAAGCCCGCCACGATCCGGTCGTCCGGGGTCTGAACTATGGTAGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCGTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCATCAAATCACATTGCCTGAATCGGCTCATCTTTTATg                                          <  2:840353/220‑1 (MQ=255)
                                                                                                                                                                                                  ggTCTGGCGTGGAAAGGAGGCATTTTCCCCCCTTGGAAAGGGAAAGAGGG‑AAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg                                          <  1:1058654/239‑1 (MQ=255)
                                                                                                                                                                                                      gggaaTGGTAAGCGGGAATTTTACCCCTTTGGAAAGGGATAGGGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt                                      <  1:500933/235‑1 (MQ=255)
                                                                                                                                                                                                        gCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCATAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAAACCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt                                      >  1:442359/1‑237 (MQ=255)
                                                                                                                                                                                                        gCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCggg                                                                                                                                                                                                            >  1:22736/1‑72 (MQ=255)
                                                                                                                                                                                                        cgtgggaacacaggtatttatcccccTGGGGAAGGGTAAGGGGA‑TAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTc                                     <  1:56002/216‑1 (MQ=255)
                                                                                                                                                                                                             gTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTGGTTGCCATGGTATTCTCCGTCCCCTTATAAAAATTTTGGGCAATGTTAACTATAGTCAGCATGCAACAAAACAAATTGCCTGAATCGGCTCATCTTTTATTCAGACCTGCaa                               >  2:500933/1‑238 (MQ=255)
                                                                                                                                                                                                              tAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGt                                                                                       >  1:614302/1‑182 (MQ=255)
                                                                                                                                                                                                               agcaaGGTATGTTAGCCCGGGGAGAAGGTATAGGGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGa                              <  1:932531/234‑1 (MQ=255)
                                                                                                                                                                                                                  cAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTTTAAAAATGTTGCGCAATGTTAACTATAGTCCGCATGCACCAAATAACATTTCCTGAATCGGCCCCTCTTTTAttcgatccgcacaaaaaa                          >  2:138407/1‑222 (MQ=255)
                                                                                                                                                                                                                      ggttttttCCGCTGGTGGAGGGAGAGGGGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAgg                       <  2:813888/232‑1 (MQ=255)
                                                                                                                                                                                                                      cATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCACCAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAggg                      >  1:1085386/1‑239 (MQ=255)
                                                                                                                                                                                                                       ttGTTACCCTCTTGTAAAGGTATAGAGGT‑AACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGt                     <  1:1047331/238‑1 (MQ=255)
                                                                                                                                                                                                                       aTGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAgggg                     >  1:436781/1‑238 (MQ=255)
                                                                                                                                                                                                                         tttctCCCCTTGGAAGGGGATGGGGGT‑GAGCCCCGCCACAATCGGGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGa                   <  2:1177611/235‑1 (MQ=255)
                                                                                                                                                                                                                           tctCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGAtt                 <  1:958269/237‑1 (MQ=255)
                                                                                                                                                                                                                            aTCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGAAACAAATCACATTTCCTGAATGGGCTCATCTTTTATGCAGTCCTGAAAAATGAGGGGGGtttt                >  2:1313922/1‑239 (MQ=255)
                                                                                                                                                                                                                              ccccTTGGGAAGGGATAGGGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTAt              <  1:449709/239‑1 (MQ=255)
                                                                                                                                                                                                                               cccTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGt                                                                                                 >  2:1200327/1‑155 (MQ=255)
                                                                                                                                                                                                                               cccTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCACAAAATCACATTTCCTGAACGGGCCCTACTTTTATGCATTCCTGCAGAATTAAGGGGGGTTTAtg             >  2:779792/1‑239 (MQ=255)
                                                                                                                                                                                                                                   tgtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGC‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAGTGTTAACTATATTCCGCATGCCACAAATCACATTGCCTGAATCGGCTCATCTTTTATGAAATCATGCACAATGAAGGGTGATTTAtgtgtt         >  2:1056411/1‑237 (MQ=255)
                                                                                                                                                                                                                                   tgtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGt                                                                                                                                                                    >  1:210674/1‑84 (MQ=255)
                                                                                                                                                                                                                                   tgtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGGGATTTAttggat         >  1:1022165/1‑234 (MQ=255)
                                                                                                                                                                                                                                   tgtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAGCCAACAACTCACATTCCCTAAATCGGACCATCTTTTATGCAGTCCTCAAAAATAAAGGGGGATTTATGGGAt         >  2:347932/1‑239 (MQ=255)
                                                                                                                                                                                                                                   tgtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCCCATCTTTTATGCAGTCCTCCAGAATAAAGGGGGATTTATGTGAt         >  2:332052/1‑239 (MQ=255)
                                                                                                                                                                                                                                    gtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGTTTGTTGTGTTGTTGCCATGGAATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTAAGTGAtt        >  1:671097/1‑239 (MQ=255)
                                                                                                                                                                                                                                    gtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGCTTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCCTCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTAttgtatt        >  2:1328128/1‑233 (MQ=255)
                                                                                                                                                                                                                                    gtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGTTCATCTTTTATGCAGCCCTGCAGAATGAAGGGTGATTTATGGGAtt        >  2:56204/1‑239 (MQ=255)
                                                                                                                                                                                                                                    gtgAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCCCACCTTTTTTGAAGTCCTCCAAAATAAAGGAGGATTTATGTGAtt        >  2:624698/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aTAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCACCAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATc  >  1:281032/1‑237 (MQ=255)
                                                                                                                                                                                                                                            aTAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAt                                                          >  1:1147435/1‑181 (MQ=255)
                                                                                                                                                                                                                                               gagGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATtgttgtgt                                                                                                                                                >  2:606298/1‑92 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          
TACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATT‑T‑CCTGCACTACAATGACCACTGGCAGTGGG‑CCAT‑CGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGT‑GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGAAGGGTGATTTATGTGATTTGCATC  >  NC_000913/4015833‑4016301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑