BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	352,846	T→G	7.1%	C47W (TGT→TGG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	352,846	0	T	G	7.1%	424.6 / 3.2	154	C47W (TGT→TGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base T (76/61); new base G (5/12); total (81/73)
Fisher's exact test for biased strand distribution p-value = 6.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AGCTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACT  >  NC_000913/352612‑353077

agCTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGGCGCCCTTTACGCAA‑ACGCCCGATCCCAGCAATCCGCCGTTTGCCCGTTGGTTTTGGGAAg                                                                                                                                                                                                                                      >  2:1314492/1‑239 (MQ=255)

agCTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGGATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAg                                                                                                                                                                                                                                      >  1:628007/1‑239 (MQ=255)

        tGGAACAGATGCCGGTCAATGAGGATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAAcc                                                                                                                                                                                                                              <  1:236915/239‑1 (MQ=255)

                     gggtaaTGAGTTTCTCCACCTTTACGTCATTTAAGTAAACGGGAGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTTTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcaca                                                                                                                                                                                                                 <  2:1314956/235‑1 (MQ=255)

                     ggTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCCTTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCCCGACCACCGCAATCCGCCGTTTGCCCGTTGGGTTTTGGAAGGCGGAACCaaaagtggccaca                                                                                                                                                                                                                 >  2:541853/1‑228 (MQ=255)

                     ggTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCCCCGTTTTCCCGTTGGTTTTGTGAAAGCCGAACCAACTTGTGCcaca                                                                                                                                                                                                                 >  2:1259053/1‑239 (MQ=255)

                                           tACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTTTGCCCCAACGCCATCGACCCcgggctggg                                                                                                                                                                                           >  1:1313068/1‑238 (MQ=255)

                                                cATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCCCGATCACAGCAAACCGCCGTTTGCCCGTTGGTTTTGTACAGGCCGAACCCACTTTTGCCCCAACCCCATCGACCCccgactgggggaaa                                                                                                                                                                                      >  2:1310587/1‑233 (MQ=255)

                                                     aaGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTTTGCCCCAAAGCCATCAACCCCGGCCTGGGGAAACCGccc                                                                                                                                                                                 >  1:1354614/1‑238 (MQ=255)

                                                     aaGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCCCAACGCCATCGACCGCTGGCTGGGGAAACAGCCa                                                                                                                                                                                 >  1:763795/1‑239 (MQ=255)

                                                     aaGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGGGAAACAGCCa                                                                                                                                                                                 >  1:1116104/1‑239 (MQ=255)

                                                          aaCGGCGGTAAGGCGTAAGTTCAACAGGCGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCCA‑ACGCTCGATCCCAGCAATCCGCCGTTTGCCCGTTGGGTTTGTGAAGGCCGAACCAACTTGTACCACAACGCCATCGCCCGCCTGCTGGGGAAACAGCAAGAgga                                                                                                                                                                            >  2:672886/1‑238 (MQ=255)

                                                          aaCGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGACCCAACTTGTGCCCCAACGCCCTCGCCCGCTGGCTGGAGAAACAGCCAGAggc                                                                                                                                                                            >  1:1306654/1‑239 (MQ=255)

                                                          aaCGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCCAAggc                                                                                                                                                                            >  1:159034/1‑239 (MQ=255)

                                                            cggcggTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCCCGGTCCCAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCACCCACCGCAGGGTGGGGaaaaaacacaagggcc                                                                                                                                                                          >  2:1052875/1‑226 (MQ=255)

                                                             ggcggTAAGGCTTAAGTTCAACAGGAGAGCATTAGGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGCggtcga                                                                                                                                                                         <  1:1165997/239‑5 (MQ=255)

                                                                 gTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGCggtcgatgg                                                                                                                                                                      <  1:961881/238‑8 (MQ=255)

                                                                  gaaGGCGGAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAggcgctggcg                                                                                                                                                                     <  1:1298587/237‑1 (MQ=255)

                                                                           aGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcaca                                                                                                                                                                                                                 >  2:842618/1‑185 (MQ=255)

                                                                           aGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCcaca                                                                                                                                                                                                                 <  1:842618/185‑1 (MQ=255)

                                                                            gTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTTCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCCCCACCCCCTCCACCCCTGGCTGGAGAAAAACCCACAgggccgggccccagttcccc                                                                                                                                                           >  2:467547/1‑220 (MQ=255)

                                                                                aaCAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCCCTGGCTGGAGCAACAGCCCACGGCGATGGCGCGGAT‑TGCCGActct                                                                                                                                                     >  1:1235915/1‑239 (MQ=255)

                                                                                aaCAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAAAGCCATCGACCGCTGGCTGGGGAAACAGCCAGAGGGCCTGGGGCGGAT‑GTCCGcctc                                                                                                                                                      >  2:1325893/1‑238 (MQ=255)

                                                                                      aaggCATTTTGTCTTTTAGCGAATTTTATCAGGGGTCGATTAACGAACCGGAGCAGGTC‑TGGGCCGAGCAGGCCCGGGGTATTGACGGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGa                                                                                                                                                <  2:1330038/235‑1 (MQ=255)

                                                                                       gagCTTTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGc                                                                                                                                                                                                                                    <  2:16086/154‑1 (MQ=255)

                                                                                       gagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCCCAACGCCATCGACCGCTGGCTGGGGAAACAGCCCAAGGGGCGGGCGCCGAT‑TGCCGCTCCTTCGGgaa                                                                                                                                              >  2:1361705/1‑239 (MQ=255)

                                                                                       gagCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGGAAAAGCCAGAGGCGCTGGCGCTGCT‑TTCCGGCCCTTCGGgaa                                                                                                                                              >  2:492422/1‑239 (MQ=255)

                                                                                        ggtcttttgtgtttgctcGAATTTTTGCAGCGTTGGTGTAACGACACGGAGCAGTTC‑TGGGCCGAGCAGGCCAGGCGTAATGACTGGGGGCGGCTCTTTGCGCAA‑ACGCTGGATCACAGCAATCCGCCGTTTGCCGGTTGGTGTTGTGAAGGGCGAACCAGCTTGAGCCCCAACGCCAGCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑GGCCGTCTCTTCGGaaa                                                                                                                                              <  2:235446/221‑1 (MQ=255)

                                                                                        ggctttttGTTTTTTAGCGAATTTTATCAGGGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGGGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGaaa                                                                                                                                              <  2:1354614/231‑1 (MQ=255)

                                                                                        ggctttATGTCTTTTAGAGAATTTTACCAGCGTTCGATTAACGAACCGGAGCAGTTC‑GGGGCCGAGCCGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGaaa                                                                                                                                              <  1:548873/234‑1 (MQ=255)

                                                                                        ggCATTTTGTTTTTTGGGGAATTTTATGAGGGTTCGATTAACGAACCGGATCAGTTC‑TGGGCCGGGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGaaa                                                                                                                                              <  2:159034/237‑1 (MQ=255)

                                                                                          cATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCACCTTTGTCCCCAACGCCATCGACCGCTGGCTGGGGAAACACCCAGAGGCCCTGGGGC‑GATGTCCCGTCCCTTCGGaaaaaa                                                                                                                                           >  2:634822/1‑236 (MQ=255)

                                                                                          cATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACCCCATCGACCCCTGGCTGGAGAAAAAGCCAAAGGCCCGGGCGCGGAT‑TGCCGGCCCTTCGGgaaaaa                                                                                                                                           >  2:1249742/1‑236 (MQ=255)

                                                                                                   ttttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAgc                                                                                                                                  <  1:1249742/239‑1 (MQ=255)

                                                                                                    tttAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAGGAaaagg                                                                                                                                  >  1:472790/1‑237 (MQ=255)

                                                                                                    tttAGCGAATTTTAGCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAgcg                                                                                                                                 <  1:467547/239‑1 (MQ=255)

                                                                                                    tttAACGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAgcg                                                                                                                                 <  1:447253/239‑1 (MQ=255)

                                                                                                    tgtAGCGAATTTTAGCAGCGTTCGATTAACGAACCGGAGCGGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACCCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTCTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTTGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAgcg                                                                                                                                 <  1:672886/237‑1 (MQ=255)

                                                                                                               ttaagagtgTTTGATTTACGTGCCGGGGCAGTTCGTGGCCGGAGC‑GGCCCGGGGTATTTGCTGGCAGCCGGCCTTTACGAAA‑ACGCTGCGTCACAGCATGCGGCAGTTTGCCCTGTGGTTTTGTGAAGGTCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCacctttacct                                                                                                                      <  2:379887/231‑1 (MQ=255)

                                                                                                                         cagattggtataagggaccggTTC‑TGGGCCGGGCCGGACCGGCGTGTTGACTGGCAGACCCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCt                                                                                                            <  2:710519/219‑1 (MQ=255)

                                                                                                                           taTTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGc                                                                                                          <  1:417324/238‑1 (MQ=255)

                                                                                                                                             cttttggggcccgggaGGCCCGGCGTTTTGACTGGGAGACGCCTTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAAcgcg                                                                                         <  2:1116104/224‑1 (MQ=255)

                                                                                                                                               tgttGGGCCGGGCGGGGCCGCGTTTTTGACTGGCAGACGCCCTTGCAGCAA‑ACGCTCGACACCAGGAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGt                                                                                       <  2:763795/236‑1 (MQ=255)

                                                                                                                                                 cggggccgggccGGCCCGGGGTTTTGACGGGCCGGCGCCCTTTACGCAA‑ACCGTCGATCACAGCAAGCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGGGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTgg                                                                                     <  2:55897/237‑1 (MQ=255)

                                                                                                                                                    ggCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAAGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAggcgct                                                                                                                                                                         >  2:359092/1‑153 (MQ=255)

                                                                                                                                                       cGAGCGGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCa                                                                                <  1:376900/238‑1 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCCCAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAAAAGAAGAAGAGCGCCCCTTTACCTTTCTTCACCGTCATGCCAAAGTGAACACGGGGGGCCCAATg                                                                             >  2:880772/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGGGAAACAGCCAGAGGCGCTGGCCCTGAT‑GGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGAGAAAGCGGGGGGCCCAATg                                                                             >  2:173981/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCTAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGCAGGCCTCAATg                                                                             >  1:798892/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TTCCGTCTCTTAGGAAACAGAAGAAAAGCCCACCTTTACCTTTCGCCAGCTGCCCGACCACATAAACCGGGTGGCCCCaagg                                                                             >  2:1013110/1‑237 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCaccttta                                                                                                                         >  1:89830/1‑195 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGaa                                                                                                  >  1:710519/1‑218 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAATTGAACGCGGTGGCCTCAATg                                                                             >  1:1217920/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATg                                                                             >  1:162343/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATg                                                                             >  1:898653/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAAAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATg                                                                             >  1:1062407/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAAAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGGGAACACGGTGGCCTCAATg                                                                             >  1:767522/1‑239 (MQ=255)

                                                                                                                                                         aGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACCCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCCCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGACGCATGACGAAGCGAACGCGGGGGCCTCaaat                                                                             >  1:1333267/1‑237 (MQ=255)

                                                                                                                                                            gggCCCGGGGTTTTGCCTGGCAGACGCCCTTTACCACA‑ACGCCCGATCACCGCAATCCCCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGTTGGCTGGGGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTg                                                                          <  2:987305/238‑1 (MQ=255)

                                                                                                                                                            gggCCCGGGGTATTGACTGGCAGACGCCCTTTACGAAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTg                                                                          <  1:584541/238‑1 (MQ=255)

                                                                                                                                                            cggCCCGGCGTTTTGGCTGGCCGGCCCCCTTTTGCCAACAGGCGCGCCCA‑AGCAATCCGGCTTTTGCCCGTTGGTTTGTTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTg                                                                          <  2:906139/238‑1 (MQ=255)

                                                                                                                                                             ggCCCGGGTGATTGCCTGGCAGTCGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGGGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTg                                                                          <  1:634822/238‑1 (MQ=255)

                                                                                                                                                                 cGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTc                                                                     <  1:1314492/239‑1 (MQ=255)

                                                                                                                                                                   gggtgttgccgggCGGACCCCCTTTACGCAA‑ACGCCTCAACACCGCAATCCGCCTGTTGCCCGGTGGTTTGGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCAt                                                                   <  2:612308/228‑1 (MQ=255)

                                                                                                                                                                   gCGTATTGACTGGCAGACGCCCTTTACGCCA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGACACAGCCAGAGGCGCTGGCGCTGCT‑TGCCGTCTCTTCGGAACCAGAAGAAGAGCGCACCTTTACCTTTTGTCAGCTGCCTGACGAAGGGAACGCGGGGGGCGCAAttgtccgtccat                                                                   >  2:1138170/1‑228 (MQ=255)

                                                                                                                                                                              ggCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGAACCTTTACATTTCGTCAGCTGCATGACGAAGTGAACGCGGGGGCCTAAATGTTGCGTTCCTTGGGGGTGCAg                                                        >  1:1324626/1‑239 (MQ=255)

                                                                                                                                                                                cAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCAGGACGAAGTGACACCGGTGGCCCACATGTTTCGTTCATTggggggggcgcg                                                      >  2:1281137/1‑230 (MQ=255)

                                                                                                                                                                                    gccctttttcccAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCgcgg                                                   <  2:401744/227‑1 (MQ=255)

                                                                                                                                                                                        cTTTTCGGAA‑ACGCGCGACCACCGCACTCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGGGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATc                                              <  2:927503/239‑1 (MQ=255)

                                                                                                                                                                                        cTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATc                                              <  1:805543/239‑1 (MQ=255)

                                                                                                                                                                                          tttcGCAA‑ACCCTCGATCCCAGCACTCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGACCCAACTTGTGCCACAACGCCCTCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCgg                                            <  2:767522/236‑1 (MQ=255)

                                                                                                                                                                                          ttACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGTCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGGGGCCTCAATGTGCCGTTCTTTGGGGGGGCAggggggggacggg                                            >  2:521424/1‑227 (MQ=255)

                                                                                                                                                                                          ttACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACCCGGGGGCCCCAATGTTGCGTTCATTGGGGGTGCAGCGCGGCGaaggg                                            >  2:1055386/1‑235 (MQ=255)

                                                                                                                                                                                           ttcGCAA‑ACGCCCGATCACAGGAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGGGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCggg                                           <  1:523531/237‑1 (MQ=255)

                                                                                                                                                                                               tgaacgcgtCGGTCCCAGCACTCCGCCGTTTGCCCGGTTGTTTTTGGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGc                                        <  2:472790/231‑1 (MQ=255)

                                                                                                                                                                                               cAA‑ACGCTGCATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCt                                       <  1:880772/239‑1 (MQ=255)

                                                                                                                                                                                                caaaggttctcccacaggatcccgcctTTTCCCCGGTGGTTTTGGGAAGGCCCAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCt                                       <  2:184452/218‑1 (MQ=255)

                                                                                                                                                                                                 a‑aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGCACCAACTTGTGCCACAACGCCATCGCCCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCCTTACCTTTCGTCAGCTGCATGACGAAGGGAACGCGGTGGCCTCCATGTTTCGGTCATTGGGGGTGCAGCGCGGCGacgggggggcgg                                     >  2:1306984/1‑228 (MQ=255)

                                                                                                                                                                                                 a‑aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCg                                                                                                                                                                                                    <  1:458161/81‑1 (MQ=255)

                                                                                                                                                                                                 a‑aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGcc                                                                                                                                                           >  2:52595/1‑121 (MQ=255)

                                                                                                                                                                                                 a‑aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGcc                                                                                                                                                           <  1:52595/121‑1 (MQ=255)

                                                                                                                                                                                                 a‑aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCTCCAGCTGCATGACGAAGTGAACCCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCCGGCGGGCGACCGGGGGGTgg                                     >  2:1234065/1‑239 (MQ=255)

                                                                                                                                                                                                   aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCCTGACGCAGTGAACGCGGTGGCCTCAATGTTGCGTTCATGGGGTGTGCAGCGGGGGGATCGGGGGGTggg                                    >  2:275888/1‑238 (MQ=255)

                                                                                                                                                                                                   aCGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAAAGCGGTGGCCTCAATGTTGCGTTCATTGGGGGGGCAGGGCGGCGATCggcgggtggg                                    >  2:668526/1‑231 (MQ=255)

                                                                                                                                                                                                          aTCTCAGCAATCCGCCGTTTGCCCGTTGGTGTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAgc                                                                                                                                  <  1:707632/138‑1 (MQ=255)

                                                                                                                                                                                                               aGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGGGTATATGCCgat                        >  1:582762/1‑239 (MQ=255)

                                                                                                                                                                                                                 cAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTTTGCAGCGCGGCGATCGGGGCCTGGGGTATATGCCgatgt                      >  1:545341/1‑238 (MQ=255)

                                                                                                                                                                                                                 cAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGAACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGGGTGCAGCGGGGCGATCGGGGGCggggtctgcctcttttaa                      >  1:269125/1‑224 (MQ=255)

                                                                                                                                                                                                                   aTCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAAGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGAACCCTTACCTTTCTTCCGCTGCAGGACGAAGAAAACCGGGTGGCCGCAATTTTGCGTTGATTGGTTGTGGAGCGGGGCGAGCGGGGGCGGGGGTTAAAGCCgaggatg                    >  2:1206326/1‑238 (MQ=255)

                                                                                                                                                                                                                    tCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGGGCTGGGGTATATGCCGCTGAtt                    >  1:157052/1‑238 (MQ=255)

                                                                                                                                                                                                                         tcttttCCCTTTGGTTTTTGGAAGGCCGAACCAACTTTGGCCACAACGCCTCCGCCCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTTCCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGACTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGaa              <  2:698648/237‑1 (MQ=255)

                                                                                                                                                                                                                            ttGCCCGTTGGTTTTGGGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAgc            <  1:173981/238‑1 (MQ=255)

                                                                                                                                                                                                                            ttGCCCGTTGGTTTGTTGAAGGCCGAACCAACTGGGGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGTCAGAGGCGCTGGCGCTGAT‑GGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGGGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAgc            <  1:99549/238‑1 (MQ=255)

                                                                                                                                                                                                                            ttGCCCGTTGGGTTTGGGAAGGCCGAACCAACTGTGGCCACAACGGCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTTGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAgc            <  1:506528/238‑1 (MQ=255)

                                                                                                                                                                                                                                   ttGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGCTCGGGTGCTGGGGGATATGCCGATGATTGCCGAAGCGCATAtt     >  1:514261/1‑238 (MQ=255)

                                                                                                                                                                                                                                    tGGTTTTGGGAAGGCCGAACCAACTTGGGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTa    <  1:374532/238‑1 (MQ=255)

                                                                                                                                                                                                                                    gggTGTTGTCAAAGCCTAACTCACTTGGGCCACAACGCCAGTGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGGTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCTGATGATTGCCGAAGCGCATATTa    <  2:1235915/237‑1 (MQ=255)

                                                                                                                                                                                                                                     ggTTTGGTGAAGGCACAACCAACTTGGGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTAct  <  1:1052875/239‑1 (MQ=255)

                                                                                                                                                                                                                                       ttttGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTg                                                                          <  2:1185053/165‑1 (MQ=255)

                                                                                                                                                                                                                                       ttttGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTg                                                                          >  1:1185053/1‑165 (MQ=255)

AGCTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGTAAGTTCAACAGGAGAGCATTATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTC‑TGGGCCGAGCAGGCCCGGCGTATTGACTGGCAGACGCCCTTTACGCAA‑ACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGAT‑TGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACT  >  NC_000913/352612‑353077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`