Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,579	T→A	18.6%	T63S (ACC→TCC)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,579	0	T	A	18.6%	189.6 / 7.5	95	T63S (ACC→TCC)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (60/10);  new base A (3/16);  total (68/27)
Fisher's exact test for biased strand distribution p-value = 1.92e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGT  >  NC_000913/4065349‑4065816                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCCATAT‑TTT‑TC‑CGCGAGA‑CGAGTAAAAATCC‑G‑G‑‑GAGACAAACCGGTAAA‑CCCGGAAAAAAACt                                                                                                                                                                                                                                           >  2:563721/1‑239 (MQ=255)        tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCCAACAAAA‑AGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑ACTTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CCCGAGT‑CGAGAAAAAATCC‑G‑GT‑CAG‑CATATCGTTAAA‑CCCGGAAAAACACT‑TCGcct                                                                                                                                                                                                                                    >  1:1240382/1‑237 (MQ=255)        tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CAAGTAAAAATCC‑G‑GT‑GAG‑CAAACCGGAAAA‑ACCGGTaaaaaaatcacacac                                                                                                                                                                                                                                    >  2:626355/1‑230 (MQ=255)                gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGC‑CGAGTAAAAATCC‑G‑GG‑GAG‑CATATCGGTAAA‑CCCGGTAAAAAACT‑TCGCCCC‑CC‑GAAAGa                                                                                                                                                                                                                         >  1:895088/1‑239 (MQ=255)                  tcatcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGACGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGGAAAAAACT‑TCGCCAC‑CA‑GAAAGATa                                                                                                                                                                                                                       >  1:448027/1‑239 (MQ=255)                   catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CCCGAGT‑CGGGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATa                                                                                                                                                                                                                       >  1:98586/1‑238 (MQ=255)                           tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGGGTAAAAATCC‑G‑GT‑GAG‑AATATCGGTAAA‑CCCGGGAAAAAACT‑TCCCCAC‑CA‑CAAAGAAAAACCCGCCa                                                                                                                                                                                                              >  2:169769/1‑239 (MQ=255)                           tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GG‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAGGATAAACCCGCCa                                                                                                                                                                                                              >  1:1268867/1‑239 (MQ=255)                             tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATa                                                                                                                                                                                                            >  1:39562/1‑239 (MQ=255)                             tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATa                                                                                                                                                                                                            >  1:517875/1‑239 (MQ=255)                             tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCAATa                                                                                                                                                                                                            >  1:240497/1‑239 (MQ=255)                             tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCAAC‑CA‑GAAAGATAATCCCGCCATa                                                                                                                                                                                                            >  1:597088/1‑239 (MQ=255)                             tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑AAAATCGGTAAA‑CACGGTAAAAAACT‑TCGCCCC‑CA‑GAAAGATAATCCCGCCAtc                                                                                                                                                                                                            >  1:639459/1‑238 (MQ=255)                             tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAAT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CACATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GACAGATAATCCCGCCAt                                                                                                                                                                                                             >  1:1248427/1‑238 (MQ=255)                              aTGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCACTAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGGAAA‑CACGGAAAAAAACA‑TCGcaac‑caagaaaaaaataccaccaaag                                                                                                                                                                                                            >  2:34682/1‑219 (MQ=255)                              aTGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCCTAg                                                                                                                                                                                                           >  1:50636/1‑239 (MQ=255)                                   aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCCC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGc                                                                                                                                                                                                     >  1:771904/1‑239 (MQ=255)                                       cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCCTAGT‑AAGCAGGc                                                                                                                                                                                                 >  1:1006218/1‑239 (MQ=255)                                       cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAgga                                                                                                                                                                                                 >  1:464937/1‑238 (MQ=255)                                            atcatAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGCGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAATT‑TCGCCCC‑AC‑GACAGATAATCCCGACATAGC‑AAGCAGGGATccc                                                                                                                                                                                            >  1:604483/1‑239 (MQ=255)                                               atAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGA‑AAGCAGGCATCCCTaa                                                                                                                                                                                         >  1:619560/1‑239 (MQ=255)                                                      gcgcGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCACTAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCGGCCATAGT‑AAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                  >  1:412407/1‑239 (MQ=255)                                                      gcgcGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                  >  1:1023835/1‑239 (MQ=255)                                                      gcgcGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGAGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGAACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGTGT‑CGGGTAAAAATCC‑G‑GT‑GAC‑CATACCGGTAAA‑AGCGGGAAAAAAAT‑TCG‑CAC‑CACGAAACACAAACCCACCCAAGA‑AAGCAGGAACCCCCACTTCAACg                                                                                                                                                                                  >  2:1186529/1‑239 (MQ=255)                                                       cgcgGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGGAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                                 >  1:733055/1‑239 (MQ=255)                                                       cgcgGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGAAAA‑CGCGGTAAAAAAAT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAAtt                                                                                                                                                                                       >  1:739512/1‑233 (MQ=255)                                                       cgcgGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATAGGAAAA‑CGCGGGAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AACCAGACATCCCCAATTCacc                                                                                                                                                                                   >  1:837584/1‑235 (MQ=255)                                                        gcgGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCCTAGT‑AACAAGGCATCCCTAATTCACCgga                                                                                                                                                                                >  1:186497/1‑238 (MQ=255)                                                        gcgGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GC‑GAG‑CATATCGGTAAA‑CCCGGTAAAAAACT‑TCGCCAC‑CA‑CAAAGAAAATCCCGCC‑CAGTAAAGCCGGCATCCCAAATTCACCgga                                                                                                                                                                                >  2:162749/1‑238 (MQ=255)                                                            gtgacatggtgtttttTA‑CCCGCAAAAAAAAGGGTGGGGGAAAAAAAC‑‑GGGGTG‑TTTCAAATTAACGCGCCCGAAACC‑GC‑TGCATAAAAAAAAAAGGGACAAAATTGGCTTTTGGGCCAAATTT‑TTT‑CC‑CTCGAGT‑CGAGAAAAAATCC‑G‑GT‑GAG‑TATCTCTGAAAA‑CGCGGAAAAAATCT‑TCGCCAC‑CA‑GAAAAATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGtat                                                                                                                                                                              <  2:733055/226‑1 (MQ=255)                                                                   tgtgtttttAACCCGGAAAAAAAAG‑GGGGGTGGCAAAAAAC‑TGGAGGG‑TTGC‑AATTAACGCCGCGGGAACC‑GC‑TGAATAAAAAATAAAGGGACGAAATTTGCCTTTAGGCCAAATTT‑TTT‑TC‑CGGGAGT‑CGAGAAAAAACCG‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                      <  1:169769/234‑1 (MQ=255)                                                                    gtcttTTTAC‑CCGGAAAACAAAG‑GGGGGTGGC‑AAAAAA‑GGGGGCG‑TTTCAAATTAACGCCGCCGGAACG‑CC‑TGAATAAAAAAAAAAGGGAAGAAATTTCCCTTAAGGCCAAATAT‑TTT‑CC‑CGCGAGT‑CGAGTAAAAACCC‑G‑GT‑GAG‑CATATCGGTAAC‑CGCGATAAAAAACT‑TCGCCAC‑CA‑AAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                    <  1:19982/239‑1 (MQ=255)                                                                     ttttttaAA‑CCGGAAAAAAAAA‑GGGGGGGGCGAAAAGG‑TGGAGAG‑TGGC‑AATAAACCCCGCCGAACCC‑AT‑GGCAAAAAAAAT‑AAGAGAAAAAATTTTGCTTTAAGGCAAATATATTT‑GC‑CGGGAAT‑CGAGAAAAAACCG‑G‑GG‑GAT‑CATACCGGTAAC‑CGCGGAAAAAAACT‑TCGCCAC‑AA‑GAAAAAAAAACCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                    <  2:50636/235‑1 (MQ=255)                                                                      ttttttTA‑CCCGCAAAAAAAA‑GGGGGGGGATGAAAAC‑TTGGGGG‑TTTA‑AATTACACCCGCCGGCACCAGA‑TGAAAAAAAAAAAAGGGGACAAATTTTGCTTTTGGCCCAAATTT‑TTT‑CC‑CGCGAG‑‑CCAAAAAAAACCC‑GTGT‑GAG‑AATATCGAAAAA‑GCAGGTAAAAACCC‑CCGCCAC‑AA‑GAAAGAAAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg                                                                                                                                                                   <  2:133193/237‑1 (MQ=255)                                                                       tttttAC‑CCGACAAAAAAGG‑GGTGGGGGAGAAAAAC‑GGGGGTG‑TTAA‑AAATACCGCCGCCGGACCC‑GT‑TAAATAAAAAAAAAAGGGACAAAATTTGCTTTTAGGCCAAATTT‑TTT‑CC‑CGAGAGG‑CGATAAAAAACCG‑G‑GT‑GAG‑CATAGCGTAAAC‑CGCGGAAAAAAACT‑TCCCCCC‑CA‑GAAAAAAAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggag                                                                                                                                                                 <  2:1273168/238‑1 (MQ=255)                                                                       tttttAA‑CCGCCAAAAAAGG‑GGTGGTTGGTAAAAAG‑GGGGGGT‑TTAA‑AATTACCCCCGCCGG‑ACC‑GCGTGAATAAAAAAAAAGGGGAAAAATTTTGCCTTTGGTCCAATTAT‑TTT‑CC‑CGCGAGT‑CGAGTAAAAATCG‑G‑GT‑GAG‑AATATCGGTAAC‑CGCGGTAAAAACCT‑CCGCCAC‑CA‑GAAAAATACTCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggag                                                                                                                                                                 <  2:30464/238‑1 (MQ=255)                                                                           tAA‑CCCGAAAAAAAAA‑GGGGGGGGGAAAAAACGGGGAGTG‑TTAA‑AATCAGCCCCGCCGGACCC‑TA‑TAAATAAAAAAAAAGGGGACAAAATTTGCCTTTAGCCCAATTAT‑TTT‑CC‑CGCGAGT‑CGAAAAAAACTCC‑G‑GG‑GAG‑CATAGCGGAAAA‑CGCGAAAAAAACCT‑CCGCCAC‑CA‑GAAAAAAAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa                                                                                                                                                              <  2:771904/239‑1 (MQ=255)                                                                            taacccgaaaaaaaaggggtgggGGAAATAAAG‑TGGGGCG‑TTGA‑AATTACCCCCGCCGAAACC‑GT‑TGCAAAAAAAAAAAAGGGACAAAATTCGCCTTTGGGCCAAATAT‑TTT‑CC‑CGCGGGT‑CGAGAAAAAACCG‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGATAAAAAACT‑TCGCCAC‑AA‑AAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa                                                                                                                                                              <  1:975823/222‑1 (MQ=255)                                                                            aa‑CCGGAAAAC‑AAA‑GGGGGGGGGGAAAAAA‑GGGGGCGTTTGC‑AATTACCCCCGACGGACCC‑GT‑TTCAAAAAAAAAAAAGGGACAAAATTCGCCTTTAGGCCAAATTT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAACCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGAAAAAAACT‑TCGCCAC‑CA‑AAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACa                                                                                                                                                            <  1:1221013/239‑1 (MQ=255)                                                                                                 gtggCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAt                                                                                                                                         >  1:1290755/1‑239 (MQ=255)                                                                                                 gtggCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCAATGCagag                                                                                                                                            >  1:7537/1‑236 (MQ=255)                                                                                                 gtggCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATAAAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGCGT‑CGAGTAAAAATCG‑G‑GT‑GAC‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGAAGACATCCCTAATTCATGGGTTTAAAATTTGAGGGACTACATAGTACCGATGCAAAGAAt                                                                                                                                         >  2:704061/1‑239 (MQ=255)                                                                                                  tggCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAtt                                                                                                                                        >  1:470503/1‑239 (MQ=255)                                                                                                   ggCGATAACC‑TGGAGAG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAACAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCCAATTCTTCGGTATAAAATTTGAGGAGATACAGAGAACCGATgaaagcactta                                                                                                                                       >  2:954305/1‑229 (MQ=255)                                                                                                       aTAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAAt                                                                                                                                    >  1:1165800/1‑238 (MQ=255)                                                                                                              attggggggttcaaatcaccgccgcCAGAACC‑GA‑GTAAAAAAAAAGAAAGGGAAAAAATTTGCACTCAGCCCAATTAT‑TTT‑TC‑CGC‑AGTGCGAGAAAAACGCC‑G‑GT‑GGT‑AATTCTGGAAAC‑CGGGGAAAAAACCT‑GCCCCAC‑CA‑AAAAAAAAAACCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGaa                                                                                                                               <  2:1023835/220‑1 (MQ=255)                                                                                                                 aGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGAAGATACAGAGTACCGATGCAGAGAATTAAATTCGAACCGAc                                                                                                                          >  1:524515/1‑239 (MQ=255)                                                                                                                   cG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCAAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGAAACAGAGAACCGATGCAAAACATTAAAttgaaaacaaggg                                                                                                                        >  2:1364726/1‑228 (MQ=255)                                                                                                                       tGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACa                                                                                                                                                            >  1:1030883/1‑200 (MQ=255)                                                                                                                       tGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTAATCGGTATAAAATTTGGGGAGATACAGAGAACCAAGGCAAAGAATTAAATTCGAAcgaaggggc                                                                                                                      >  2:723118/1‑230 (MQ=255)                                                                                                                             ttAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGGGCATTAAATTCGAACCGACGTCGCccat                                                                                                                 >  1:980085/1‑238 (MQ=255)                                                                                                                                  gccgccGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAAGCATTAAATTGGAAcccaaggcccccaaaaccaa                                                                                                           >  2:826693/1‑221 (MQ=255)                                                                                                                                                c‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTc                                                                                               >  1:925453/1‑238 (MQ=255)                                                                                                                                                      tataAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAg                                                                                         >  1:1151985/1‑239 (MQ=255)                                                                                                                                                              aaaGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGGGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtt                                                                                 >  1:1268877/1‑239 (MQ=255)                                                                                                                                                              aaaGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCTTAGGCGATTTTTTCCCGCAGCTTCAGtt                                                                                 >  1:986035/1‑239 (MQ=255)                                                                                                                                                                aGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGcc                                                                                                                                                                                                                                     >  1:856462/1‑92 (MQ=255)                                                                                                                                                                aGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGcc                                                                                                                                                                                                                                     <  2:856462/92‑1 (MQ=255)                                                                                                                                                                aGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGATTACCGATGCAGAGCATTAAATTCGacc                                                                                                                              >  1:1338950/1‑190 (MQ=255)                                                                                                                                                                aGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGCCGTCGCCCATACCATAGGCGGTTTTTTCCCGCAGATTCAGtttt                                                                               >  1:619133/1‑239 (MQ=255)                                                                                                                                                                aGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                               >  1:397345/1‑239 (MQ=255)                                                                                                                                                                aGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCCCCCAAACCAAAGAGAATTTTTTCCCCCAAATTAAGttta                                                                               >  2:676978/1‑238 (MQ=255)                                                                                                                                                                     cGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAGGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATg                                                                           >  1:317240/1‑238 (MQ=255)                                                                                                                                                                       aattttgtttttAGCCCAAATTT‑TTT‑CC‑CGCGGAT‑CAAGAAAAAACCG‑G‑GT‑GAA‑GATAGCGGTAAG‑GGAGGAAAAAACCC‑CCGCCCA‑CA‑GAAAAAAAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                        <  2:470503/230‑1 (MQ=255)                                                                                                                                                                        aaTTTGGTTTTAGTCACAAATT‑TTT‑TCGCGCGAGA‑GAAGAAAAAACCG‑G‑GG‑AAA‑CATGTGGGAAAA‑GGCGGTAAAAAACC‑TCCCCCC‑AA‑AAAAAAAAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                        <  2:1006218/239‑1 (MQ=255)                                                                                                                                                                             gCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAACGATGCAAAGAATTAAATTCGAACCGACGTGTCCCATCCCAAAGGCGATTTTTTCCCGCAGATTAAGTTTTAATTACAGGGGAt                                                                  >  2:1204868/1‑239 (MQ=255)                                                                                                                                                                                 ttgggCCCAATAT‑TTC‑CC‑CGCGATT‑CGAGAAAAAACCC‑G‑GT‑GAG‑CATATGGGTAAA‑CGCGAAAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATg                                                              <  1:723118/236‑1 (MQ=255)                                                                                                                                                                                  tAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑ACGCAGGCATCCCTAAtt                                                                                                                                                                                       >  1:447234/1‑117 (MQ=255)                                                                                                                                                                                  tAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAAt                                                                                                                                    >  1:978430/1‑168 (MQ=255)                                                                                                                                                                                  tAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGACCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAAATTCAGTTTTAATGACAAGGGAtaaag                                                              >  2:625689/1‑234 (MQ=255)                                                                                                                                                                                   aagCCCAATTT‑TTTATC‑AGAAAGT‑GGAGAAAAAATCG‑G‑GT‑GGG‑CATATGGTAAAA‑CGGGTAAAAAAACT‑CCTCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATgg                                                             <  1:1186529/237‑1 (MQ=255)                                                                                                                                                                                         aaTAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CG‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGTCCCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGGTTCAGTTTTAATGTCAATGACTAATGGTCaaaaa                                                      >  2:511990/1‑235 (MQ=255)                                                                                                                                                                                             tttttttttccccggagtagaaaaaaaaCC‑G‑GTGGAG‑AATAGGGGTAAC‑CG‑GGAAAAAAACTCCCCCCAA‑CA‑AAAAGATACCCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGAc                                                       <  2:1268877/217‑1 (MQ=255)                                                                                                                                                                                                  ttccctagagt‑cgaaaAAAAATCG‑G‑GG‑GAA‑CAGTGCGGAAAA‑CGGGAAAAAAAACT‑TCCCCAC‑AA‑AAAAAAAAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGGCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                <  2:53810/232‑1 (MQ=255)                                                                                                                                                                                                   tttcgcgcaggagaatAAAAACGC‑G‑GT‑GAG‑ATTATGGGTAAA‑C‑CGGGAAAAAACC‑CCCCCCCACA‑AAAGAATATCCCCGCCATAGG‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                <  2:1165800/224‑1 (MQ=255)                                                                                                                                                                                                   ttcccggagt‑tgaatAAAAAACT‑G‑GTGGAG‑CTTATGGTAAAA‑CGCGGAAAAAAACT‑CCACCAA‑AA‑GAAAAAAAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                <  2:597088/233‑1 (MQ=255)                                                                                                                                                                                                    tgtgcgtgagggtttAAAAACCCGG‑GT‑GAC‑CATACCGATAAA‑CGCGTAAAAAAACA‑CCGCCCC‑TA‑AAAAAAAAATGCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                <  2:1243793/225‑1 (MQ=255)                                                                                                                                                                                                     gccgggagcgaaaaaaaaaCCC‑G‑GT‑GAG‑CATA‑CGGAAAACCGGGGTAAAAACCT‑CCACCAC‑CA‑AAAAGATACTCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                              <  1:826693/225‑1 (MQ=255)                                                                                                                                                                                                        cgagt‑cgagtAAAAATCC‑G‑GT‑GGG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAGGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTc                                             >  1:211813/1‑236 (MQ=255)                                                                                                                                                                                                        cgagt‑cgagtAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAG‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCACTAATTCATCGGTATAAAATTTGAGGAGCTACAGAGTACCGATGCAGACGATTAAATTCGAACCGACGCCTCCCATACCCAAGGGGATTTTTTCCCCCACATTCAGTTTTAATGCCAGAGGATGTGGGTCAGACaaaacaaacccta                                          >  2:1162272/1‑230 (MQ=255)                                                                                                                                                                                                        cgagt‑cgagtAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCCGATTCAGTTTTAAGGTCAGTGGATTATGGTCAGACATAATCACTCCtt                                          >  1:1243793/1‑239 (MQ=255)                                                                                                                                                                                                        cgagt‑cgagtAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGATTCAGTTTTAATTTCAGTGGATTATGGTCAGACATAAACAACCCtt                                          >  2:704542/1‑239 (MQ=255)                                                                                                                                                                                                                    aaaaCCG‑G‑GG‑GAG‑CATACGGGAAAA‑CGGGGAAAAAAACT‑GCGCACC‑CA‑AAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCg                               <  1:625689/239‑1 (MQ=255)                                                                                                                                                                                                                          ataaacaggaacattccgatAAA‑CGCGGAAAAAAACT‑CCCACAC‑AA‑GAAAAAAAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCa                             <  2:1372020/220‑1 (MQ=255)                                                                                                                                                                                                                            g‑gT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                        >  1:593557/1‑239 (MQ=255)                                                                                                                                                                                                                              gggaaG‑CATATGGTTAAA‑CGCGTAAAAAATCT‑CCGCACC‑AA‑AAAAGATAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                        <  1:511990/235‑1 (MQ=255)                                                                                                                                                                                                                                  aG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCAAACCATAGGCGATTTTTTCCCGCAAATTCAGTTTTAAAGTCAGTGGTTTATGTTCAGACATAATCACTCCTTATAAAGCCCCCCAgggggcgtt                    >  2:919500/1‑233 (MQ=255)                                                                                                                                                                                                                                   agacattgcggaaacccgggTAAAAAAC‑‑TCCCCACAAA‑GAAATAAAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcgtc                      <  1:954305/220‑1 (MQ=255)                                                                                                                                                                                                                                       aacgggTAAA‑CGGGATAAAACACC‑TCGCCAA‑GA‑AAAATATAACCCCGTCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                <  1:34682/235‑1 (MQ=255)                                                                                                                                                                                                                                        ttgcgaaaaaccgaatAAAAATCT‑TCCCCCC‑AA‑TAAAGATAACCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATCCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                <  2:517875/224‑1 (MQ=255)                                                                                                                                                                                                                                             tAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAAAGCCCCGCAGGGCGTCGtt                   >  1:1372020/1‑230 (MQ=255)                                                                                                                                                                                                                                             tAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTCAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGCCGTCGCCCATACCATAGGGGATTTTTTACAGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCAATCCTTATAACCCCCCGCAGGGCGGGGTttataattat          >  2:987466/1‑239 (MQ=255)                                                                                                                                                                                                                                               aa‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATACCTCCTTATAACGCCCCCCAGGGCGTCGTttatatttata         >  2:570590/1‑237 (MQ=255)                                                                                                                                                                                                                                                     ggTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCg   >  2:428882/1‑239 (MQ=255)                                                                                                                                                                                                                                                      gTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGCTTATGGTCAGACTTAATCACTCCTTATAACGCCCACCAGGGCGTCGTTTATTATTATTACGCCGt  >  1:214409/1‑239 (MQ=255)                                                                                                                                                                                                                                                      gTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCATATAACGCCCCGCAGGGGGTCGTTTATTATTATTACTCCGt  >  2:836255/1‑239 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAA‑CCGGCAAACAAAA‑GGTGGTGGCGATAAAC‑TGGAGCG‑TTGC‑AATTAACGCCGCCGGAACC‑GC‑TGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAT‑TTT‑TC‑CGCGAGT‑CGAGTAAAAATCC‑G‑GT‑GAG‑CATATCGGTAAA‑CGCGGTAAAAAACT‑TCGCCAC‑CA‑GAAAGATAATCCCGCCATAGT‑AAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGT  >  NC_000913/4065349‑4065816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑