BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,107,661	T→G	16.2%	G37G (GGT→GGG)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,107,661	0	T	G	16.2%	320.0 / 6.8	135	G37G (GGT→GGG)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base T (26/77); new base G (27/2); total (56/79)
Fisher's exact test for biased strand distribution p-value = 2.40e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGA  >  NC_000913/4107428‑4107890

gacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGCAGTAATGGGGAtttt                                                                                                                                                                                                                                          >  1:356939/1‑238 (MQ=255)

 cccctgttCAATTCCGCAGGATGTGATTGTTTTTATTTTTGCACTTTCGTTGGATCACTTCGATGTTCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTAt                                                                                                                                                                                                                                         <  2:571423/232‑1 (MQ=255)

 accttaATCAATTCAGCAGGAAGTTATTGTTATTCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTAt                                                                                                                                                                                                                                         <  2:454291/239‑1 (MQ=255)

   ctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATg                                                                                                                                                                                                                                        <  1:1057787/238‑1 (MQ=255)

    tgaATCAATTCAGCAGGAATTGATTGTTATACTATTTGCAAATTCGTTGGATCACTTCGATGTGAAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAc                                                                                                                                                                                                                                      <  2:963202/239‑1 (MQ=255)

           aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCAATTCGCGGGGTTGTTCG‑GTTTGGGCTGACAGAAGGTCTGGAAGTAATGGGAATTTATGAC‑GGCTa                                                                                                                                                                                                                                >  1:1293139/1‑237 (MQ=255)

           aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGGGATGCGCCAGGCATGAACGCCGGAATTCGCGGGGGTGTTCG‑TTCTTCGCTGGACGAAAGCCTGGAAGTAATGGGGATTTATGAC‑GGCTa                                                                                                                                                                                                                                >  1:933082/1‑237 (MQ=255)

               agcagGAATGTATTGTTCTACTATTTGCACATTCGTTGGATCACTTCGCTGGGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGTTTATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑Tggg                                                                                                                                                                                                                         <  1:1180379/239‑1 (MQ=255)

                 cagGAAGTTTTTGTTTTTCTTTTTGCCAATTCTGTGTATCCCTTCGATGTTCAAGAAGACTTCCGGCAACAGATTTCATTTGGCATTCCAAAGTTCAGAGGTAGTCATTATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTc                                                                                                                                                                                                                       <  2:402938/239‑1 (MQ=255)

                 cagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGGGCCAGGCATGAAGGCCCCAATTTCCGGGGGTTTTCG‑GTTTTCGCTGGCAGAAGGGCTGGAAGGAATGGGGATTTTTGACGGGGTATC‑Tgggg                                                                                                                                                                                                                        >  2:1309729/1‑238 (MQ=255)

                  agGAAGTTTTTTTTTTTCTTTTTGCAAATTCGTTGGATCACTTCGATGTGCAAGAAGCCTTCCGGAAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTc                                                                                                                                                                                                                       <  2:1274537/238‑1 (MQ=255)

                  agGAAGTGATTTTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTc                                                                                                                                                                                                                       <  1:211859/238‑1 (MQ=255)

                  agGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTc                                                                                                                                                                                                                       <  1:811693/238‑1 (MQ=255)

                  agGAAGGGATTGGTATACTATTTGCCAATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTc                                                                                                                                                                                                                       <  1:790061/238‑1 (MQ=255)

                     gaGTTATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTc                                                                                                                                                                                                                       <  1:938817/234‑1 (MQ=255)

                      aGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACCGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGCCAAGCGGCGGTGTTTCCCCCGGCCGTAACGCCGCAATTTCAGGGGTTGTTCG‑TTCTGCGCTGCCAGAAGGCTGGGGAGAAATGGGGATTTATTGC‑GGCTATCTTGGGCCTGtg                                                                                                                                                                                                                   >  1:1371034/1‑238 (MQ=255)

                         ttttGTTATACTTTTTCCAAATGTGTTTGATTATTTCGATGTGCAAGAAGGCTGCCGGAAACAGATTTCATTTGGCAATCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCTAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGaa                                                                                                                                                                                                               <  2:283076/237‑1 (MQ=255)

                         gATTGTTTTATTATTTGCACATTCGTTGGATCCCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGaa                                                                                                                                                                                                               <  2:500276/239‑1 (MQ=255)

                         gATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCAATTCGCGGGGTTGGTCG‑GTCTGCGCTGACAGAAGGGCTGGAAGGAATGGGGATTTATGAC‑GGCCATC‑TGGGGTGGTATGaa                                                                                                                                                                                                               >  1:1322760/1‑239 (MQ=255)

                          aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGCGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTTTTCGCTGACAGAAGGTCTGGAAGTAATGGGGATTTTTGAC‑GGCTATC‑TGGGTCTGGATGaaa                                                                                                                                                                                                              >  1:364625/1‑238 (MQ=255)

                            tGTTATTCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             <  1:76739/238‑1 (MQ=255)

                               ttttctctttGCCCTTTCTTTGGATTCCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATGCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑Gt                                                                                                                                                                                                        <  2:1116252/232‑1 (MQ=255)

                               tattCTAATTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑Gt                                                                                                                                                                                                        <  1:283565/239‑1 (MQ=255)

                               tataCTATTTGCACATTCGTTGGATCACGTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTGCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑Gt                                                                                                                                                                                                        <  1:701638/239‑1 (MQ=255)

                                tttcTATTTGCACATTCGTTGGTTCACTTCGATGGTGAAGAAGACTTCCGGCAACAGATTGCATTTTGCATTCCAAAGGTCAGAGGTAGTCATGATTAAGAAAATCGGTGGGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTa                                                                                                                                                                                                       <  2:356939/236‑1 (MQ=255)

                                ataCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTa                                                                                                                                                                                                       <  1:724117/239‑1 (MQ=255)

                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGCCAAGCGGCGGTGATGCGCCAGGCCTGAAGGCCGCCATT‑TCGGGGGTATTCGCGTCTGCCTCGCCAGCAGGTCTGGGCAGAATGGGGGTTTTTAAC‑GGCTATC‑Cggggcgttatggaaaaggtgaggaga                                                                                                                                                                                                  >  2:283565/1‑216 (MQ=255)

                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCTCGGGGTTGTTCG‑GTTTGCGCTGCAAGAAGGGCTGGAAGTAATGGGGATTTATGAC‑GGCTATC‑TGGGTCTGTTTGAAGACC‑GTATGGtcc                                                                                                                                                                                                 >  1:826644/1‑237 (MQ=255)

                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGGATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             >  1:427313/1‑228 (MQ=255)

                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑GTTTGCGCTGCCCGAAGGGCTGGTAGTAATGGGGATTTTTGAC‑GGCAATT‑TGGGGCTGGAAGAAAACC‑CTAttgga                                                                                                                                                                                                  >  2:76739/1‑234 (MQ=255)

                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCC‑GTCTGCGCTGGCCAAAAGGCTGGGAGAAATGGGCGTTTATTAC‑GGCTCAC‑TGGGtgcggttggaacgcgtaaggta                                                                                                                                                                                                   >  1:1222603/1‑217 (MQ=255)

                                      tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGATAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGAGATGCGCCAGGCATGAAAGCCCCAATTCGCGGGGTTGTTCG‑TTTTGCGCTGACAGAAAGGCTTGAAGAAAATGGAATTTTTGAC‑GGCTATA‑CGGGGCTGTTTGACAACC‑GCATGGTAc                                                                                                                                                                                                 >  2:431437/1‑239 (MQ=255)

                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGTTGAGCCAGGCATGAACGCCGCAATTTCCGGGGTTGTTCG‑TTCTTGGCCGACAGAAGGTCGGGAAGCATGTGGGATTTTTGAT‑GGCTTTC‑TGGGTTGGTAGGGAAACC‑GTATgggaaga                                                                                                                                                                                               >  2:754214/1‑234 (MQ=255)

                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACCGAAGGGCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGGCTGTATGAAGACC‑GTATGGTACa                                                                                                                                                                                                >  1:877287/1‑238 (MQ=255)

                                        tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCACTGACAGAAGGTCTGGAAGTAATGGGGCTTTATGAC‑GGCTATC‑TGGGTCTGAATGAAGACC‑GTATGGTaaa                                                                                                                                                                                                >  1:568732/1‑236 (MQ=255)

                                         gCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGc                                                                                                                                                                                              <  1:1144416/239‑1 (MQ=255)

                                         gCACATTCGTTGGATCAATTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGc                                                                                                                                                                                              <  1:477416/239‑1 (MQ=255)

                                           acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTa                                                                                                                                                                                            <  1:222125/239‑1 (MQ=255)

                                            caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTa                                                                                                                                                                                            <  1:61579/238‑1 (MQ=255)

                                             cttCGTTGGTTCCCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGa                                                                                                                                                                                          <  2:630924/238‑1 (MQ=255)

                                             aTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAATGTTCAGAGGGAGTCATGATTAAGAAAATCGGTGTGTTGACAACCGGCGGCGGTGGGCCATGCAAGAACTCCGCAATTCACCGGGTGGTTCG‑ATCTGGGCGGGCGGCAGGCCTGGAAGTAATGGGTATTTATGTC‑GGGGTTC‑TGGGGCCGTTTGAAGGCC‑GAATGGTataaataga                                                                                                                                                                                          >  2:414640/1‑231 (MQ=255)

                                             aTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             >  1:998537/1‑221 (MQ=255)

                                             aTTCGTTGGATCAATTCGATGGGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGa                                                                                                                                                                                          <  1:902271/239‑1 (MQ=255)

                                              ttCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGGATTTTTGAC‑GGCCATC‑TGGGTCTGTATGAAGACC‑GTTTGGGACAGCTaaa                                                                                                                                                                                          >  1:572361/1‑236 (MQ=255)

                                              ttCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCCATTCGCGGGGTTGTTCG‑TTTTGCGCTGCCGGAAGGGCTGGGGGTAATGGGGATTTATGAC‑GGCGATC‑TGGGGCTGATTGAGGACC‑GTATggacaagccaaa                                                                                                                                                                                          >  2:759368/1‑228 (MQ=255)

                                                 ggtGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGt                                                                                                                                                                                      <  1:321622/237‑1 (MQ=255)

                                                         aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACCCCGCAATTCGCGGGGTTGTTTG‑TTCTGCGCGGGCCGGAGGGCCGGGAGGAATGGGGGTTTTTTAC‑GGCGTAC‑TGGGTCTGTATTAAGAAC‑GGATGGTGCAGCTAGACCGTTACAGCgg                                                                                                                                                                              >  2:321622/1‑238 (MQ=255)

                                                             cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACCGAAGGGCTGGAAGTAATGGGGATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCggg                                                                                                                                                                             >  1:1249024/1‑234 (MQ=255)

                                                                    cAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGaa                                                                                                                                                                                                               <  1:1022007/196‑1 (MQ=255)

                                                                           tcTTCCGTCATCAGTTTTCATTTTGGTTTCCAAAGTGCAGAGGTAGTCATGAATAAGAAAATCGGTTGGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCGGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCg                                                                                                                                                           <  2:754987/238‑1 (MQ=255)

                                                                              cccGGCAACTGTTTTTATTTTGCTTTCCAAAGTGAAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGTTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑GTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTgg                                                                                                                                                        <  2:1369090/238‑1 (MQ=255)

                                                                               ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             >  1:631106/1‑187 (MQ=255)

                                                                               ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGGTGTTCG‑TTTTGGGCTGTCGGAAGGTCTGGAAGGAATGGGGGTTTTTGAG‑GGCTGTC‑TGGGTCTGTTTGAGTGCC‑GTATGGGGCGTGCAGGCCGTTACAGAGTGTCtcagagatgaaaacgcggg                                                                                                                                                        >  2:1375693/1‑221 (MQ=255)

                                                                               ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGGCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             <  2:631106/187‑1 (MQ=255)

                                                                                   caaaggATTTTCTTTTGGATTCCAAAGTTAAGGGGTGGTCAGGATTAAGAAAAGCGGTGTTGTGACAAGCGGCGGTGATGCGCCAGGCATGAACGGCGCAATGCGCGGTGTTGTTCG‑GTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑Gt                                                                                                                                                   <  2:744708/233‑1 (MQ=255)

                                                                                                 ttGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCg                                                                                                                                                      >  1:1231174/1‑222 (MQ=255)

                                                                                                 ttGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCg                                                                                                                                                      <  2:1231174/222‑1 (MQ=255)

                                                                                                                        gTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGGCAGAAGGTCTGGAAGGAATGGGGATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTAAAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GGCAGTTACCCGGGTCTACGC‑GTGTTCCCGGattctcggc                                                                                                            >  2:851070/1‑231 (MQ=255)

                                                                                                                        gTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATT‑CGGGTCTGTATGAAGACC‑GTATGGTAAAGCTAGACCGTTACAGCGTGTCTGACAGTATCCA‑CCGTTGCG‑GTCAGTTCCCCGGTTTTCGGC‑CG‑TTCCCCGA‑ATTCcgaca                                                                                                          >  1:840285/1‑236 (MQ=255)

                                                                                                                        gTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGGTTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCGGTATGAAGACC‑GGATGGGACAGCTAGACCGTTACAGCGTGTGTGACAAGATCAA‑CCGTGGGG‑GGACGGTCCCCGGTTTTGGGC‑GT‑GTCCCGGACATTCcgcg                                                                                                           >  2:586672/1‑239 (MQ=255)

                                                                                                                        gTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGGCTGGGAGTAATGGGTATTTTTGAC‑GGCTATC‑TTGGGCCTTATTAAGACCGGTATGGAAAAGTAAGACCGTAACCGGGGGCTTGAC‑GGATTAA‑ACCGGGCG‑GGACGTTCCCCGGGTCTTGGC‑GT‑TTCCCGGA‑AATCcccg                                                                                                           >  2:951640/1‑238 (MQ=255)

                                                                                                                        gTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGGCTGGAAGTAATGGGGATTTTTGTC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGGACCGCTATACCGTTACAGCGTGTCTGACATGATCAA‑CCGGGGCG‑GGACGTTCCTCGGTCTTGCGG‑GT‑TTCCCGGA‑AATCCCcga                                                                                                          >  2:335209/1‑239 (MQ=255)

                                                                                                                             gATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGGATTTATGAC‑GGCTCTC‑TGGGGCTGTATGAAGACCGGTATGG‑ACAGCAAGACCGTTTCAACGGGTTTGGCCTGTTCAC‑CCGTGGCGCGCATGTTCC‑CGGTTTCTCCC‑GT‑TTCCCGGA‑ATTcccgccacgaa                                                                                                     >  2:615139/1‑230 (MQ=255)

                                                                                                                             gATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAAGCCGCAATTTGCGGGGTTGGTCG‑TTCTTGGCTGCCAGAAGGGCTGGAAGTAATGGGTATTTATGTC‑GGCTACC‑TGGGGCGGGAGGAAGACC‑GTATGGGACAGCTAGACCGTTCAAGAGGGTCTGACAAGATACA‑CCCGGGGG‑GGACGTGCCCCCGTTCGGCGC‑GT‑TGCCCCGA‑AATCCGCGGCggga                                                                                                     >  2:788096/1‑239 (MQ=255)

                                                                                                                                tAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCCCGGGGGTGTTCG‑TTTTGCGCTGACAGAAGGGCTGGAAGGAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATTAGGACC‑GTATGGTACAACTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GGAGGTTCCTCCGGTCCGCGC‑GG‑TTCCCCGG‑ATACCCCGGAGAGaaa                                                                                                   >  2:1358550/1‑237 (MQ=255)

                                                                                                                                        tCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGGTCTGCGC‑GT‑TTCCCGGA‑ATTTCGCGGCGAGAACATcccgccc                                                                                          >  1:701384/1‑234 (MQ=255)

                                                                                                                                         cGGTGTGTTGACAAGCGGCGGTGATGGGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCg                                                                                         <  1:1309729/239‑1 (MQ=255)

                                                                                                                                         cGGTGTGTTGACAAGCGGCGGGGATGCTCCAGGCATGACCGCCGCAATTCGCGGGGTTGTTCG‑TTCGGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑GGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCg                                                                                         <  1:557426/239‑1 (MQ=255)

                                                                                                                                            tgtgtTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGAc                                                                                                                                                                       <  2:453553/163‑1 (MQ=255)

                                                                                                                                            tgtgtTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGGGTCTGAc                                                                                                                                                                       >  1:453553/1‑163 (MQ=255)

                                                                                                                                              tgtTGACAAGCGGCGGTGATGCGCCAGGGCTGAACGGCGCAATTCGCGGGGTTGTTCG‑TTCTGGGCCGGCAGAAGGGCTGGAAGAAATGGGGATTTATGGC‑GGGTATC‑TGGGGCTTGATGAAGACC‑GGATGGTACAGGCTGACCGGTGCAGCGGGTCTGACATGGTTAACCCGTGCGG‑GGACGTTCC‑CGGGGCTGTGCGGT‑TTCCCGGA‑ATCCCGCGCCGaaaaaacgcaccccggggc                                                                                     >  2:168075/1‑224 (MQ=255)

                                                                                                                                                    cAATCGGCGGTGATGCGCCAGTCATGAACGCCGCAATTCGCGGTGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGaa                                                                              <  1:936851/239‑1 (MQ=255)

                                                                                                                                                    cAAGGGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑GTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGaa                                                                              <  1:759368/239‑1 (MQ=255)

                                                                                                                                                               gagggcgcgggCATGAACGGCGTAATTGGGGGGGTTTTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGCC‑GGCTATC‑TGGGTTTTTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGaaaa                                                                    <  2:364625/229‑1 (MQ=255)

                                                                                                                                                                 tGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGaaaaaa                                                                  <  1:1236807/238‑1 (MQ=255)

                                                                                                                                                                  gcgcCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACg                                                                <  1:615139/239‑1 (MQ=255)

                                                                                                                                                                       gggCATGAACGCCGCAATTCGGGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTAGTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTa                                                           <  1:1375693/238‑1 (MQ=255)

                                                                                                                                                                        ggCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GG‑TTCCCGGA‑ATTCCGCGACGAGAACACCCCCGACTGGGCCATCGAAAACCTGAAAAAAAGTgtgat                                                          >  1:357675/1‑235 (MQ=255)

                                                                                                                                                                        ggCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCCCGGTTCTGCGC‑CT‑TTCCCGGA‑ATTCCGCCACGAGAACAGCCCCGCCCGGGCTATCGAAAACCTGAAAAAAccggggaa                                                          >  2:1093973/1‑232 (MQ=255)

                                                                                                                                                                              aaggccgcAATGCGCGGGGTTGTTCG‑TTCTGCGCTGAAAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGAcg                                                     <  1:1289402/235‑1 (MQ=255)

                                                                                                                                                                               ccgccgcAATTCTGGGGGTTTTTCT‑TTCGGCGCTGACAGAAGGTTTGGAAGTAATGGTTATTTATGGC‑GGCTATC‑TGGGTCTGTATGAAGGCC‑GTATGGTACAGCTAGACCGTTGCAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGAcgcg                                                   <  2:438903/238‑1 (MQ=255)

                                                                                                                                                                                   cgcAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGCAGGTCTGGAAGTAATGGGTATTTATGCC‑GGCTATC‑TGGGGCTGAATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTAGGTTCCTCGGGTCTGCTC‑GT‑TTCACGGA‑GTTTCGGTACGAGAACGTCCGCGCCCTGGCAGTCGAAAACCTGAAAAAACggcggatcacagacccgg                                               >  2:1101794/1‑222 (MQ=255)

                                                                                                                                                                                   cgcAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCCCGCCGGGGCGATCGAAAACCTGAAAAAACGGGGGAACGACCGGCtgg                                               >  2:1256597/1‑239 (MQ=255)

                                                                                                                                                                                   cgcAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GGACGTTCCCCGGATCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGCCGGCAACAACCGCGCCGTGGCTGTCGAAAACCGCAAAAAACgggagatggacggcgggg                                               >  2:1023899/1‑222 (MQ=255)

                                                                                                                                                                                                   gttcg‑ttcTGCGCTGACAGAAGGTTTGGAAGTAATGGGTATTTATGCC‑GGCTATC‑TGGGTCTGTATGAAGCCC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGa                               <  1:136640/239‑1 (MQ=255)

                                                                                                                                                                                                    ggtaaaagagacaggACAGAAGGTCTGGAAGTAAGGGGGTTTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGGGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGa                               <  2:807173/225‑1 (MQ=255)

                                                                                                                                                                                                     tttgtttgtgctttaaaGATGGTCTGGAAGTAAGGGGTTTTTATGAC‑GGCTATC‑TGGGTCTTTATGAAGACC‑GTATGGTACAGATAGACCGTTACAGCGTGTCTGAAATGATCAA‑CCGTGGCG‑GTACGTTCCGCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGAc                              <  2:12922/223‑1 (MQ=255)

                                                                                                                                                                                                      tctttcTGGGCTTACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTAGC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGGTAGGCCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACg                             <  2:1061277/236‑1 (MQ=255)

                                                                                                                                                                                                        tttcTGCCCTGACAGAAGTTCTGGAATTTATGGGTTTTTATGAC‑GGCTATC‑GGGGTTTGTAAGAAGACC‑GTATTGTACAGCTAGACCGTTACAGCTTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGt                           <  2:568732/238‑1 (MQ=255)

                                                                                                                                                                                                           cTGCGCGTACAGAAGGTCTGGAATGAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTcc                        <  1:851070/239‑1 (MQ=255)

                                                                                                                                                                                                            tGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGGGAACATCCGCGCCGTGGCTATCGAAAGCCTGAACAAACGGGGTATCGACGCGCTGTGGGTTATcggcggggtggggttg                        >  1:1179175/1‑228 (MQ=255)

                                                                                                                                                                                                             tggcTGGCAGAAGGTCTGGAAGTAATGGGTATTTATGCC‑GGCTATG‑TGGGTCTGTATGAAGCCC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGACCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTa                      <  2:871585/237‑1 (MQ=255)

                                                                                                                                                                                                               gcTGACAGAAGTTCTGGAAGTAATGGGTATTTAGGGC‑GTCTATC‑TGGGTTTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGGCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACa                    <  2:18451/239‑1 (MQ=255)

                                                                                                                                                                                                               gcTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             <  1:253092/60‑1 (MQ=255)

                                                                                                                                                                                                               gcTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGa                                                                                                                                                                                                             >  2:253092/1‑60 (MQ=255)

                                                                                                                                                                                                                cTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑TTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACa                    <  1:128025/238‑1 (MQ=255)

                                                                                                                                                                                                                        aGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGCGGGATCGACGCCCTGGGGGTTATcggggggggcgagtccgtcaacggggggc           >  1:805124/1‑216 (MQ=255)

                                                                                                                                                                                                                                 aGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTg   <  1:1040588/238‑1 (MQ=255)

                                                                                                                                                                                                                                 aGTAATGGGTATTTAGTAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGa  <  1:806169/239‑1 (MQ=255)

                                                                                                                                                                                                                                  atAAGGGGTATTTATGAC‑GGCTATC‑TGGGGTTGTAAGAAGACC‑GTATGGTAAAGCTAGACCGTTACAGCGTGTCTGACAGGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGa  <  2:1249024/237‑1 (MQ=255)

GACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCG‑TTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGAC‑GGCTATC‑TGGGTCTGTATGAAGACC‑GTATGGTACAGCTAGACCGTTACAGCGTGTCTGACATGATCAA‑CCGTGGCG‑GTACGTTCCTCGGTTCTGCGC‑GT‑TTCCCGGA‑ATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGA  >  NC_000913/4107428‑4107890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`