BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,156,549	T→C	13.6%	S180P (TCC→CCC)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,549	0	T	C	13.6%	282.8 / 3.1	107	S180P (TCC→CCC)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base T (64/25); new base C (2/16); total (66/41)
Fisher's exact test for biased strand distribution p-value = 1.99e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGC  >  NC_000913/4156317‑4156784

gtttCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑CGGGCGC‑GGCTCTGATTTTGCTCTCCGAc                                                                                                                                                                                                                                           >  1:385455/1‑238 (MQ=255)

                ggtggCGTCAAAGGGCCCCAGGTTGGTGAAGAGTTGGGTCATTTTGGCCTGGGGCCGCCAGGTTCCGCTAAGCTTATCCACTCCTTGCTGGACAACGGGTATCT‑GCCGGTT‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCg                                                                                                                                                                                                                           <  2:207659/234‑1 (MQ=255)

                 acacCGTCAAAGTGCCCCAGCTTGATGAAGAGTTAGTGCATGTTGGGCTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGCCCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGa                                                                                                                                                                                                                          <  2:757215/238‑1 (MQ=255)

                 aCAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGa                                                                                                                                                                                                                          <  1:115198/238‑1 (MQ=255)

                               aCCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGGCAAAGGGCAAc                                                                                                                                                                                                            >  1:1164630/1‑238 (MQ=255)

                                 ccAGCTTGATGAAGAGTTAGGTTATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCa                                                                                                                                                                                                         <  1:840918/239‑1 (MQ=255)

                                 ccAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCa                                                                                                                                                                                                         <  1:173439/239‑1 (MQ=255)

                                    gCTTGATGAAGAGTTTGGTCATGTTGGGCTGGCGCAGCCAGGTTCGCCTAAGCTTAGCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTg                                                                                                                                                                                                      <  1:1368251/239‑1 (MQ=255)

                                    gCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCGGATTTTGCGCTCCGCCGTCAGCGGCATTCTGGCCGGCAAAGGGGACCGGAtt                                                                                                                                                                                                       >  1:1078902/1‑238 (MQ=255)

                                         atgaAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCCGGTTTTGCTTCCCCGCTGCAGGGGCATTTCCCCCGGCAAAGGGCAACGCATGGCGGaa                                                                                                                                                                                                 >  2:626330/1‑239 (MQ=255)

                                         atgaAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACCC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGCCAACGGGCAACGCATTGCCGaa                                                                                                                                                                                                 >  1:414761/1‑239 (MQ=255)

                                               agTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGGCAAAGGGCAACGCATTGCCGAAATGAc                                                                                                                                                                                            >  1:1356701/1‑238 (MQ=255)

                                               agTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAAGGCATTGCCGAAATGAc                                                                                                                                                                                            >  1:528313/1‑238 (MQ=255)

                                                   aGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAccg‑cc                                                                                                                                                                                       >  1:1145575/1‑238 (MQ=255)

                                                         tGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCACCGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGTCGGCAAAGGGCACGGCATTGCCGCAATGACCG‑CCGCGAAAg                                                                                                                                                                                >  1:36927/1‑239 (MQ=255)

                                                         tGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGaaa                                                                                                                                                                                 >  1:479652/1‑238 (MQ=255)

                                                         tGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGaaa                                                                                                                                                                                 >  1:1278698/1‑238 (MQ=255)

                                                          gTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCGGATTTTGCTCTCCGACGTCACCGGCATTCCCGACGGCAAAGGGCCACCCATTGCCGAAATGACCG‑CCCCGAAAga                                                                                                                                                                               >  2:1164755/1‑238 (MQ=255)

                                                           ttGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCCGCGGCATTCCTCACGGCAAAGGGCAACGCATTGCCCAAAAGAccc‑ccggcaaaaca                                                                                                                                                                              >  2:27570/1‑231 (MQ=255)

                                                             tgcctgggccccccAGGTCCGCCTAGGCTTTTCACCTCCTTCCTGGAGACGGGTTAAC‑‑GCCGGGGTGGTGGCCCCATGGGCGTAACAGACGAA‑GGGAACCTGAGGACCGTCAATGCCGCCCGGGCGGCACCGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCCCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGa                                                                                                                                                                            <  2:1137975/227‑1 (MQ=255)

                                                                cTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGACCTGATTTTGCTCTCCGCCGCAAGCGGCATTCTCGACGGGCACGGGCACCCCATTTCCGAAATGACCG‑CCCCGAAAGCAGaaaa                                                                                                                                                                         >  2:1056896/1‑237 (MQ=255)

                                                                cTGGCGCAGCCAGGTTCGCCTAAGCTTATAAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGCCGCAACAGACGAA‑GGGCAACTGATGAACGTCATGGCCGACCAGGCGGCAACGGCGCT‑AGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTTCCTGAGGACACAGGGATTCTAGACGGCTACGGGCAAGACATTTCTGAACTAGCCG‑CCGCGAACGGAGaaca                                                                                                                                                                         >  2:91557/1‑239 (MQ=255)

                                                                   gcgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCTTTGCCGAAATGACGG‑CCGCGAAGACAGacca                                                                                                                                                                         >  1:148852/1‑236 (MQ=255)

                                                                   gcgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGCAAGCAGaaca                                                                                                                                                                         >  1:710343/1‑236 (MQ=255)

                                                                   gcgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCCGaaca                                                                                                                                                                         >  1:989580/1‑236 (MQ=255)

                                                                   gcgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCCCGAAAGCGGaaca                                                                                                                                                                         >  1:526579/1‑236 (MQ=255)

                                                                    cgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGGCAAAGGGCAACGCCTTGCCGAAATGACCG‑CCGCGCAAGCAGAACAACTg                                                                                                                                                                     >  1:134464/1‑239 (MQ=255)

                                                                     ccccccAGTTTCCTCTATGCTTTACACCCCCTTGGTGGGAAACGGTTATCT‑GCCGGTG‑GCCGGCCCCATTGGCGAAACAGACGAA‑GGGCAACTGATGAACGCCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCCCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGa                                                                                                                                                                    <  2:710343/235‑1 (MQ=255)

                                                                      cgggcgggtccgctgaaGGTTAACAACTCCTTGCTGGAG‑ACGGTTTACTGGCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAG‑GGGCAAGTGATGAACGTCATGGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGA‑GGATCTGATTTTGCTCCCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATGGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGa                                                                                                                                                                    <  2:958981/223‑1 (MQ=255)

                                                                        gCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACCGATTg                                                                                                                                                                 >  1:243778/1‑239 (MQ=255)

                                                                         ccAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGa                                                                                                                                                                >  1:1303091/1‑239 (MQ=255)

                                                                             gttgctcTAAGCTTCACAACCCCTTGTGGGAAAACGGTTTTGG‑GCCGGGG‑GGCAGGCACATTGGGGAAAAAGACGAA‑GGGCACATGATGAACGTAAAGGCCGACCAGGGGGCAACGGCGCT‑GGCGGAAACGC‑GGGGCGG‑GGATCTGATTTTGCTCCCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCAAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCa                                                                                                                                                             <  2:479652/232‑1 (MQ=255)

                                                                             gTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCCAAGGGCAACGCATTGCCGAAATGACCG‑CCCCGAAACGAGAAAAACTAATGGAGAA‑g                                                                                                                                                           >  2:576281/1‑239 (MQ=255)

                                                                                  ccTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTCCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCat                                                                                                                                                      >  1:1365465/1‑239 (MQ=255)

                                                                                   cTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCACCGCATTGCCGAACTGACCGACCGC‑AAAGCAGAACAACCGGTTAAGGA‑gggactt                                                                                                                                                     >  2:75179/1‑235 (MQ=255)

                                                                                         ttATCAACCCCGTGCTGGAAAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACt                                                                                                                                               <  1:27570/239‑1 (MQ=255)

                                                                                         ttAACAACTCCTTGCGGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCTTTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCGGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACt                                                                                                                                               <  1:726942/239‑1 (MQ=255)

                                                                                           ttccactcttttggggAGAACGTTTATCT‑GCGGGTG‑TCCAGCCCCATGGGGTTAACGAACCAG‑GGGCAACTGATTAACGTCACTGCCGCCCGGGCGGCAACGGCGCT‑GGCGGCAACGC‑GGGGCGC‑GGATCGGATTTTGCTCTCCGACGTCAGCGGCATTCGCGACGGCAAAGGGAAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGa                                                                                                                                             <  2:371514/225‑1 (MQ=255)

                                                                                           tcccccTCCTTGGTGGAGAACGTTTATCT‑GCCGGTG‑GTCAGCCCCATTGGGGTAAAAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCG‑GGCGGCAACGC‑GGGGCGG‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGa                                                                                                                                             <  2:1303091/234‑1 (MQ=255)

                                                                                           aTCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCCTTCTCGACGGCAAAGGGCAACGCATTGCCGAACTGACCG‑CCGCGAAAGCGGAACACCTGGTTGAGCA‑GGGCATTATTACtt                                                                                                                                              >  1:17451/1‑237 (MQ=255)

                                                                                           aTCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACAAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCCC‑GGATCTGATTTTGCTCTCCCGCGTTAGCGGCATTCTCGACGGGCAAGGGCCACGCATTGCCGGAATACCCC‑CCGGGGAAACCAAACAACCTATTAGGCA‑gggacaaaataactg                                                                                                                                             >  2:187995/1‑227 (MQ=255)

                                                                                                tcctttgtggaaaccgggTTCTCT‑CCGGGT‑‑GTCAGCGCCAGTGGCTTAACAGACGAA‑GGGCAACTGATGAACGTAAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATTTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCAt                                                                                                                                       <  1:72079/223‑1 (MQ=255)

                                                                                                  ccTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCCGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGAGTTTGCTCTCCGACGTCAGCGGCATGCTCGACGGCAAAGGGCAACGACTTACCGCACTTACCG‑CACGGAAAGCCGAACAACTGATTGACCA‑GGGCAATATACATGACGGCaga                                                                                                                                      >  2:1139588/1‑237 (MQ=255)

                                                                                                  ccTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCCGAACACCTGATTGAGCA‑GGGCATTATTACt                                                                                                                                               >  1:774437/1‑230 (MQ=255)

                                                                                                  ccTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGGGCA‑GGGCATTATTACTGACGGCATg                                                                                                                                      >  1:738146/1‑239 (MQ=255)

                                                                                                   cTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGGCAAAGGGCAACGCATTGCCGCAATGACCG‑CCGCGAAAGCAGAACAACTGATTGGGCA‑GGGCATTATTACTGCCGGCATGa                                                                                                                                     >  1:7476/1‑239 (MQ=255)

                                                                                                   cTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGa                                                                                                                                     >  1:1108730/1‑239 (MQ=255)

                                                                                                     tGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGACAGCAGAACAACTGCTTGAGCA‑GGGCATTATTACTGACGGCATGATa                                                                                                                                   >  1:662081/1‑239 (MQ=255)

                                                                                                     tGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATa                                                                                                                                   >  1:1126991/1‑239 (MQ=255)

                                                                                                      gCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCCTTGCCGAAATGACCC‑CCGCGAAAGCCGAACAAAAG‑TTGTGGATGGGCATTATTACTGAggggaggaaag                                                                                                                                  >  2:1349550/1‑231 (MQ=255)

                                                                                                             aaCGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGCAAGCAGAACAACTGATTTGGCA‑GGGCATTATTACTGACGGCCTGATagggaaagg                                                                                                                           >  1:990516/1‑238 (MQ=255)

                                                                                                                  tttttt‑GCCGGGG‑GTCAGCT‑CAATGGGGCGACAGGGCAAGGGGCAATTAAAGAACTGCAATGCCGCCAAGGGGGCCACGGCGGT‑GGGGGCAACGC‑GGGGCGC‑GGATCTGATTTTGCCCCCCGAAGTCGCCGGCATTCGCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAAcg                                                                                                                      <  2:1365465/234‑1 (MQ=255)

                                                                                                                             gtg‑gtCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGa                                                                                                            >  1:482544/1‑239 (MQ=255)

                                                                                                                                 gtCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGc                                                                                                         >  1:656965/1‑239 (MQ=255)

                                                                                                                                      cctctgtggggtaaaagcgtga‑GGGCAACTGATGAACGTCAAGGCCCGCAGGGCGGCACCGGCGCT‑GGCGGCACCGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGAGGGCAAAGGGAAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGccc                                                                                                    <  2:134464/218‑1 (MQ=255)

                                                                                                                                                gTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGaa                                                                                                                                                                                  >  2:609540/1‑152 (MQ=255)

                                                                                                                                                gTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGTCGGCATGATCGTGAAAGTGAACGCGGCCCTGGATGCGGCCCG‑CACGCTgg                                                                                          >  1:626801/1‑238 (MQ=255)

                                                                                                                                                gTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGa                                                                                                                                     >  1:1095117/1‑196 (MQ=255)

                                                                                                                                                gTAACAGACGAA‑GGGCAACGGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGaa                                                                                                                                                                                  <  1:609540/152‑1 (MQ=255)

                                                                                                                                                   aCAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGGCTGATTACTGACGGCAGGATAGGGAAAATGAACGCGGCGCTGGATGCGGCCAG‑CACGCTGGGcc                                                                                       >  1:20390/1‑238 (MQ=255)

                                                                                                                                                   aCAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGGGACAGCGGCGCTGGATGCGGCCCG‑CACGCTGGGcc                                                                                       >  1:777849/1‑238 (MQ=255)

                                                                                                                                                        cGAA‑GGGCAACGGAGGAACGTAAATGCCGACCAGGCGGCAACGGCGCG‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGt                                                                                     <  1:890109/235‑1 (MQ=255)

                                                                                                                                                         gAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACcg‑ccgc                                                                                                                                                                                     >  2:922546/1‑140 (MQ=255)

                                                                                                                                                         gAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACcg‑ccgc                                                                                                                                                                                     <  1:922546/140‑1 (MQ=255)

                                                                                                                                                         gAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGgcgc                                                                                                                >  1:629857/1‑208 (MQ=255)

                                                                                                                                                         gAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGgcgc                                                                                                                <  2:629857/208‑1 (MQ=255)

                                                                                                                                                             gggCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGCCGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCCTGATAGGGAAAGGGAAAGCGGCGCTGGGCGGGGCCGGACACGCGGGGCCgccgggaag                                                                              >  2:1233797/1‑231 (MQ=255)

                                                                                                                                                             gggCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑GACGCTGGGCCGTCCGGgaga                                                                             >  1:540189/1‑239 (MQ=255)

                                                                                                                                                                  aCTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATc                                                                         >  1:981665/1‑238 (MQ=255)

                                                                                                                                                                    tgaagaccgtaattccccaCCGGGCGGCAGGGGGGGG‑GGCGGCCAGGC‑GGGGCGG‑GGATCTGATTTTCCTCTCCGAGGTCAGCGGCATGCGGGCCGGAAAGGGGCAACGGATTGCCAAAGTGACCG‑CCGCGAAAGCAGAGCAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGcc                                                                      <  2:719341/221‑1 (MQ=255)

                                                                                                                                                                        gaACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTTACGGCATGATAGTGAAAGTTAACGCGGCGCGGGATGTGGCCCC‑CCCGCTGGGCCGCCCGGGAGAGATCGcccccg                                                                  >  2:1129590/1‑238 (MQ=255)

                                                                                                                                                                             tCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCg                                                                                                                                                                                                                           <  1:100831/84‑1 (MQ=255)

                                                                                                                                                                             tCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCg                                                                                                                                                                                                                           >  2:100831/1‑84 (MQ=255)

                                                                                                                                                                                      aCCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTGGATATGCCCTCCTGGCGTCATTCggg                                                     >  1:920797/1‑237 (MQ=255)

                                                                                                                                                                                      aCCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCCTGCggg                                                     >  1:348300/1‑237 (MQ=255)

                                                                                                                                                                                       ccccggcggccccgcgcTGGGCGGCACGGC‑TGGGGGG‑GGTTTTTATTTTGACCCCCGAGCGCAGCGGCATTCCCGCGGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGGGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGag                                                    <  2:540189/226‑1 (MQ=255)

                                                                                                                                                                                        cgggtgcgacggggggt‑GGCGGC‑ACGCGTGGGGGC‑GGATTTGTTTTGCCTCCCGGAGCTCGGCGGCAGTCGGGCCAGAAAAGGACAACGTTTTGTCGAACTGCCCG‑CCGCGAAAGCAGAACGACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTTGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGagca                                                  <  2:243778/223‑1 (MQ=255)

                                                                                                                                                                                            cggcAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCt                                               >  1:1152164/1‑238 (MQ=255)

                                                                                                                                                                                                  ccgcgcC‑GGGGGCAACG‑‑TGGGGGCGGGTTCTGTTTTGCCCCCCCGACGCAAGCGGCATTCTCGACGGCAAGGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  2:1108730/237‑1 (MQ=255)

                                                                                                                                                                                                  cGGCGCT‑GGCGGCAAGGC‑GGGGCGC‑GGATCTGGTTTTGCTCTCCGACGTCAGCGGCATTCCCGACGGCAAAGGGCACCGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  1:1233797/239‑1 (MQ=255)

                                                                                                                                                                                                    gggggcggggggcacgggtGGGGGC‑GGATTTGATTTTGCCCCCCGAGGTCAGGGGCATTCTCGGGGGCAAAGGGCAACGCATTGCATAAATGCCCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCCGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  2:526579/221‑1 (MQ=255)

                                                                                                                                                                                                    ggctt‑GGGGGCAACGC‑GGGGGGC‑GGATCGGATTTTGCCCCCCGCCGTCAGCGGAATTCCCGACGGAAAAGGGCAACGATTGGCCAAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  1:60199/233‑1 (MQ=255)

                                                                                                                                                                                                    gcggT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  1:1062304/237‑1 (MQ=255)

                                                                                                                                                                                                    cggcggtggggtcacgggggggCGG‑GGATGTTATTTTGCTCCGCGAGTGCGGGGGCATTCGCGACTGCAAAGGGCAACGCATTGCAGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  2:1187394/220‑1 (MQ=255)

                                                                                                                                                                                                     gacggggcggcCACGC‑TGGGCGG‑GGATCTGATTTTGCTCCCCGACGGCAGGGGCATTCTCGACGGCAAAGGGCAACGCATGGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                        <  1:554562/232‑1 (MQ=255)

                                                                                                                                                                                                     cgcT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGACCAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGATGGGCCGGCCGGAGGATAAGGCCTCCTGGCGGCATGGGGGGCAGCTTCCggaccgg                                     >  2:96868/1‑234 (MQ=255)

                                                                                                                                                                                                        ctggggcaaaCGG‑TGGGCGG‑GGATCTGATTTTGCTCCCCGACTGCAGGGGCATTCCCGACGGAAAAGGGCAACGCATTGCCGAAAGGGCCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGtt                                   <  1:626330/231‑1 (MQ=255)

                                                                                                                                                                                                            cggccccGC‑TGGGGGT‑GGTTTTGTTTTTGCTCCCCGACGTCAGCGGCATTCTCGCCGGAAAAGGGAAACGCATTGCCAAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                <  2:1152164/232‑1 (MQ=255)

                                                                                                                                                                                                                aaCGG‑TGGGCGG‑GGATTTGTTTTTGCCCCCCGACGTCGGCGGCATTCCAGACGGAAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                            <  1:576281/238‑1 (MQ=255)

                                                                                                                                                                                                                      tGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAGATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGGCCGGTAGATATTGCCTCCTGGGGTCATGCGGGGCCGCTTCCGGCACCGTTTAACggccggc                        >  2:554562/1‑232 (MQ=255)

                                                                                                                                                                                                                      tGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCGG‑CCCGCTTGGCCGGCCGGTAGATATAGCCTCCTGGCGTCATGCGGGGCAGCTTCGGGCACTGTTTAACGGTATGCCg                      >  1:646673/1‑239 (MQ=255)

                                                                                                                                                                                                                      tGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCg                      >  2:209020/1‑239 (MQ=255)

                                                                                                                                                                                                                          gccgggATTTTATTTTGCTCCCCGACGTCAGCGGAATTCTCGGCGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGAt                    <  1:1349550/234‑1 (MQ=255)

                                                                                                                                                                                                                              ggATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCG‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATCCGGCCCG‑CACGCTGGGCCGTCCGGTACAAATCGCCTCCTGGGGTCATTGGGGGCGGCTTCCGGCACTGTTTAAGAGAATCCCGAGGGGTa               >  2:282709/1‑239 (MQ=255)

                                                                                                                                                                                                                               gATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCa                                                                                                                                                             >  2:726240/1‑98 (MQ=255)

                                                                                                                                                                                                                               gATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCa                                                                                                                                                             <  1:726240/98‑1 (MQ=255)

                                                                                                                                                                                                                               gATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGGAGATATCGCCTCCTGGCGTCATGCGGGGCAGCTTCCGCCCCGGTTTacgggaaggccgtgggggag              >  2:1048434/1‑220 (MQ=255)

                                                                                                                                                                                                                                          gCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CCCGCTGGGCCGTCCGGGAGATATCGCCTCCGGGGGTCATGCGGCGCAGCTTCCGGCCCTGTTTTACGGTAAGCCCATGGGTCCGCGGGttttc    >  2:1008440/1‑237 (MQ=255)

                                                                                                                                                                                                                                          gCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATAGCGCCTCCTGGCGCCATGCGGGGCAGCTTCCGGCACGGTTTAAGGGTATGGCGATGGGTCCGCGGGttttga   >  2:51862/1‑237 (MQ=255)

                                                                                                                                                                                                                                           ctccCCGACGGCAGGGGCATTCTCGACGGAAAAGGGCAACGCATTGCCAAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGc  <  1:1026612/239‑1 (MQ=255)

GTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAACGGTTATCT‑GCCGGTG‑GTCAGCTCCATTGGCGTAACAGACGAA‑GGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCT‑GGCGGCAACGC‑TGGGCGC‑GGATCTGATTTTGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCG‑CCGCGAAAGCAGAACAACTGATTGAGCA‑GGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCG‑CACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGC  >  NC_000913/4156317‑4156784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`