BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,800	C→A	11.8%	A152S (GCG→TCG)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,800	0	C	A	11.8%	407.7 / 3.4	141	A152S (GCG→TCG)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base C (73/48); new base A (2/18); total (75/66)
Fisher's exact test for biased strand distribution p-value = 4.16e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGC  >  NC_000913/4241562‑4242037

ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑AAA‑TGAGCTGGAGCCAGTTCCGc                                                                                                                                                                                                                                                >  2:68328/1‑239 (MQ=255)

ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGCATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCATCAGTATTCAG‑CAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGAGGCCAGTTTCGCa                                                                                                                                                                                                                                               >  1:935550/1‑239 (MQ=255)

   cgcgACACCAGCCCGCGAGGACTTTCTGGCACGGTAGAAAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAat                                                                                                                                                                                                                                             <  2:351056/239‑1 (MQ=255)

        cATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACa                                                                                                                                                                                                                                        <  2:1128478/239‑1 (MQ=255)

          tCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACAtt                                                                                                                                                                                                                                      <  1:1028252/239‑1 (MQ=255)

               cgacGAAGACTTTCTGGCACGGTATCCAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACAC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGc                                                                                                                                                                                                                                   <  1:889025/234‑1 (MQ=255)

               cagcGGGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCga                                                                                                                                                                                                                                 <  2:864324/239‑1 (MQ=255)

                  cGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCgaggg                                                                                                                                                                                                                              >  1:825347/1‑237 (MQ=255)

                  cGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGagag                                                                                                                                                                                                                              >  1:1095816/1‑239 (MQ=255)

                       gttttATGGCGCGGTAAAAAGGAGAATTAAGAACAGCAGGGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTAAGTATTCAGCCAGCTGGAATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                                                                                                                                                                                          <  2:941667/236‑1 (MQ=255)

                       ccTTTTTGGACCGGTATTCAGCAGCATTAAGAACAGCAGGGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                                                                                                                                                                                          <  2:862156/237‑1 (MQ=255)

                       atttttTGGAACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                                                                                                                                                                                          <  2:1278778/236‑1 (MQ=255)

                       aCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                                                                                                                                                                                          <  2:1289757/238‑1 (MQ=255)

                               gCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTaa                                                                                                                                                                                                                  >  1:1309926/1‑238 (MQ=255)

                                cACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGGGAGCATTGAGGCTAAAc                                                                                                                                                                                                                >  1:956141/1‑239 (MQ=255)

                                 aCGGTATACAGAAGCATTAAGAACAGCAGTGCAGGGATGCATTCCGAGGCAAACATATAACGCCAGCCGGCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAAcc                                                                                                                                                                                                               <  1:374116/239‑1 (MQ=255)

                                  cGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAAcc                                                                                                                                                                                                               <  1:1275422/238‑1 (MQ=255)

                                     tataCAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGACATATACATTGGCGAGAGCATTTAGGCCAAACCaaa                                                                                                                                                                                                            >  2:1034161/1‑237 (MQ=255)

                                             agcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAACTAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGCGGCTAAACCAACGCCCAtcc                                                                                                                                                                                                    >  1:349861/1‑236 (MQ=255)

                                              gcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTTAGGCTAAACCAACGCCAATAcc                                                                                                                                                                                                   >  1:521570/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGTAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAAACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGGGCATTTAGGCTAAAACAACCCCAAAAcccc                                                                                                                                                                                                 >  2:282411/1‑237 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACa                                                                                                                                                                                                                                        >  1:845154/1‑200 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACa                                                                                                                                                                                                                                        <  2:845154/200‑1 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                                                                                                                                                                                          <  2:1244524/214‑1 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                                                                                                                                                                                          >  1:1244524/1‑214 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAAc                                                                                                                                                                                                                >  1:853619/1‑224 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                  >  1:247034/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGc                                                                                                                                                                                                 >  1:66360/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGAATTGAGGCAAAAACAAACCCAAAACCga                                                                                                                                                                                                 >  2:590167/1‑238 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATAAACATTGGCGAGAGAATTAAGGCTAAACCAACGCCAAAACCGc                                                                                                                                                                                                 >  2:628645/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCAAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCACCGCCAATACCGc                                                                                                                                                                                                 >  1:605023/1‑239 (MQ=255)

                                               caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTACCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATAAATGGGCGAGAGCATTGAGGCTAAAAAAACGcacataaac                                                                                                                                                                                                  >  2:605583/1‑230 (MQ=255)

                                                      aaCAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAat                                                                                                                                                                                                                                             >  1:346753/1‑188 (MQ=255)

                                                      aaCAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGCGCATTGAGGCTAAACCAACGCCAATACCGCCaataat                                                                                                                                                                                          >  1:929200/1‑239 (MQ=255)

                                                         agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGCGAGCATTAAGGCTAAACCAACGCCAATACCGCCAATAATGCg                                                                                                                                                                                       >  1:704770/1‑239 (MQ=255)

                                                           cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGCAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCTCGA‑ATA‑TGAGCTGAAGCCAGTTCCGCAATATACATTGGCGAGAGCCTTGAGGCTCAACCAAACCCAAAACCCCCAAAAATggcaa                                                                                                                                                                                     >  2:239683/1‑235 (MQ=255)

                                                              tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa                                                                                                                                                                                  >  1:141698/1‑239 (MQ=255)

                                                              tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTAAGGCTAAACCAACGCCAAAACCCCCAAAAAAACGataaa                                                                                                                                                                                  >  2:663251/1‑239 (MQ=255)

                                                                    gatctatcccggagCAAAAATATACCGCCAGCCGTCAGTATTCACCCAGCTGACATCACCGGACCGGGAAAAAAAATAG‑TTTACGC‑AGTAAACAAAAAGTTCCCGAAAAATAGGCGAAAAC‑TGTT‑T‑AAAAGAGACCA‑GTTCCCCGCGA‑ATA‑TGAGTGGGAGCAAGTTCCGCAATATACATGGGCGAGAGCATAGAGGCTAAACCACCGCCAATACCGCCAATAATGCGATAAATAACa                                                                                                                                                                             <  2:1358955/225‑1 (MQ=255)

                                                                     aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACGCCCCATAATGCAAAAAATAACaaa                                                                                                                                                                           >  1:584727/1‑239 (MQ=255)

                                                                        cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAAAAAAtt                                                                                                                                                                         >  1:1358955/1‑238 (MQ=255)

                                                                          ttCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGCGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACaactgtct                                                                                                                                                                      >  1:1316403/1‑233 (MQ=255)

                                                                             cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGGGAGCATTGAGGCTAAACCAAACCCACAACCGCCAAAAATGCGATAAAAAAAAAATTCCGGGa                                                                                                                                                                   >  2:207808/1‑239 (MQ=255)

                                                                             cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGATGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAACAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAAAAAAGCGATAAATAAAAAAATcccgac                                                                                                                                                                   >  2:311672/1‑235 (MQ=255)

                                                                             cGAGACAAAAATAGAACGCCAGCCGTAATTATCCACCCAGCTGGCAGCCCCGGAACGGGAAATAAAAAAG‑TTTACGA‑AGAAAACAAAAATTGGCCAAAAAATAACCGAAAAC‑TGGT‑T‑AAAAGCACCCA‑GTTCCCCGGGA‑AGA‑TGAGCTGGAGCCAGTCCCGCAATATACATTGGCGAGAGCATGGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGa                                                                                                                                                                   <  2:1309926/239‑1 (MQ=255)

                                                                                     aCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACCAAACCt                                                                                                                                                           >  1:1169291/1‑239 (MQ=255)

                                                                                       atatAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGAAATAAAATAT‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAACC‑TGTT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGACCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                       <  1:889481/225‑1 (MQ=255)

                                                                                           aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaataa                                                                                                                                                                               >  1:616844/1‑213 (MQ=255)

                                                                                                 aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTGAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAACTGCCAGATAAACa                                                                                                                                               >  1:1133447/1‑239 (MQ=255)

                                                                                                     tgtttttatCAGCCAGGGAGCAACGCCGAAACGGAAAATAAAAATG‑TTTAGCC‑AAAAAACAAAAAGTTG‑CCAAAAAAAATTGCAAAC‑GGGTGT‑AAAGGAAACAA‑GTTTCCCGGGA‑ATA‑AGAACGGGGACCAGTCACGAAAATTACATGGGCGAAAAAATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGca                                                                                                                                          <  2:1176355/231‑1 (MQ=255)

                                                                                                     gtcatgttccagccaggTGGCATCACCGGAACGGGCAAAAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCAAAAAATAATCGAAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCAA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGca                                                                                                                                          <  1:282411/230‑1 (MQ=255)

                                                                                                     gTCAGTATTCACCCAGCGGGCATCACGGGAACGGGCAAAAAAATAG‑TTTACGA‑AGTAAACTAAAAGTTGCCCAAAAATAATCGCAAAC‑TGGT‑A‑AAAAGAGCCCA‑TTTCCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGca                                                                                                                                          <  1:239683/239‑1 (MQ=255)

                                                                                                        attttccagcaagcggaaaTCCCCGGAACGGGAAAAAAAATAG‑TTGACGA‑AGAAAAATAAAAGGTGCCCAAAAAGAATCGAAAAC‑TGGT‑A‑AAAAGAGACCA‑TTTCCCCGCGA‑AGA‑GGAGCGGGAGCCAGTCCCGAAATATAAATGGGCGAAAGAATGGAGGCTAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAg                                                                                                                                       <  2:751370/221‑1 (MQ=255)

                                                                                                        aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAACGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGACCCAGTTCCGCAATATACATTGGCGAGAGAATTGAGGCTAAACCAACGCCCATACCGCCAATAATCCGATAAATAACAAATACCGGGACATAACCTACCAGATAAACA‑GGcccct                                                                                                                                       >  1:742661/1‑235 (MQ=255)

                                                                                                               gacacaggtgcacaaCCGGAACGGGAAATAAATTAT‑TTGACGC‑AGAAAACTAAAAGTAGCCAGAAAATACTCGAAAAC‑TGGT‑T‑AAAAGAGGCCA‑GTTCCCCGAGA‑ATT‑TGAGCTGGAGCAAGTCCCGCAATATACATTGGCGAGAGCATTGAGGCTAACCCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                <  1:1346150/225‑1 (MQ=255)

                                                                                                               caccatcTGGCACCCCCAGAAGGGACAAAAAAATAA‑TCTACGA‑AGAAAAATAAAAGTAGCTCAAAATAAATCGAAAAA‑TGTT‑A‑AAAAGGGACCA‑TTTTCCCACAG‑ATA‑TGAGCGGGACCGAGTTCCGCAATATATATTGGCGAGATTATATGGGTTAAACAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                <  2:1086463/237‑1 (MQ=255)

                                                                                                               agccagcGGGAATCCCGGGAACGGACAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTGGCCCGAAAATAATCGAAAAC‑TGGT‑T‑AAAAGGGACCA‑GTTCCCCGCGA‑ATA‑TGAGGTGGAGCCATTTCCGAAATATACATTGGCGAGAGCATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                <  1:605583/239‑1 (MQ=255)

                                                                                                               acccagcgagaaTCACCGGAACGGGCAATAAAATAG‑TTTACGA‑AGAAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑A‑AAAAGAGCCCA‑GTTCCCCGCGA‑ATA‑GGAGCGGGAGCCAGTCCCGCAATATACATTGGCGAGAGCATTGAGGCTAACCCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                <  1:987730/228‑1 (MQ=255)

                                                                                                                ccccgggtggaatctcgggaaggggAATAAAAATG‑TTTTAGCAAGAAAACAAAAAGTGGCCGAAAAATAATAGCAAGC‑TGTT‑T‑AAAAGAGACCA‑TTGTCCGGAGA‑TTA‑GGGGCTGGAGCCAGTCCCGAAATACACATGGGCGAGAGCATAGAGGATAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                <  2:66360/218‑1 (MQ=255)

                                                                                                                acccggtgggaacccccgAAAGGGGAAAAAAAAAT‑TTTTCGCAAGAAAATAAAAATTTGCCAGAAAATAACCGCAACG‑TGGT‑A‑AAAAGAGCCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATAAATGGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                <  2:521570/222‑1 (MQ=255)

                                                                                                                 ccagcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAACTAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGCCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCCATAAATAACAAATTCCGGGCCATAACCTGCCAGCACAACC‑GGCACAACGTTTTCCg                                                                                                                              >  1:1141565/1‑239 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCATTATCCCCAATATTGCGATAAATAACAAATTCCGGCCCATAACCTTCCAGAAAAAAA‑GGAACAGTGTTGCCCGGGt                                                                                                                           >  2:968645/1‑239 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAgtg                                                                                                                                     >  1:728733/1‑229 (MQ=255)

                                                                                                                    gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTCCCAGATAAACA‑GGCACAAGGTTGTCCGGGt                                                                                                                           >  2:981172/1‑239 (MQ=255)

                                                                                                                         aagtcccGGAACGGGGAAAAAAAAAGTTTTACGA‑GGAAAGAAAAAAGTTGCACGAAAATAAGCG‑AAAC‑CGGT‑TAAAAAGAGCCCG‑TTTCCCCGAGA‑ATA‑GGAGCTGGAGCAAGTCCCGAAATATACATTGGCGAGAGCATTGAGGCTAACCAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTtat                                                                                                                       <  2:206934/234‑1 (MQ=255)

                                                                                                                          aTCCCCGGAACGGGCAA‑AAAATAGTTTTCCGA‑ATAAAATTAAAAGTTCCCGAAAAAAAATCG‑AAAC‑CGGT‑TAAAAAGAGACAG‑GTTCCCCGGGA‑ATT‑GGAGCTGGGGCCAGTTCCGAAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTtata                                                                                                                      <  1:628645/238‑1 (MQ=255)

                                                                                                                              ccGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAACTAACAAATTCCGGGACATAACCCGCCCGCTAAACA‑AGCAAAGTGTTGTCCGGGTTTTTAAAgga                                                                                                                 >  2:1287603/1‑238 (MQ=255)

                                                                                                                                      ggCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTaaaa                                                                                                             >  1:206934/1‑235 (MQ=255)

                                                                                                                                      gcaaaaaaaaTTG‑TTTACGA‑ATAAAACTAAAATTTCCCAAAAAATAAGTGCAACC‑TGGT‑T‑AAAAGAAACAG‑GTCTCCCGCGA‑ATT‑GGGGCTGGCGCCACTTCCGCAATAAACATTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAAcc                                                                                                           <  2:1236911/231‑1 (MQ=255)

                                                                                                                                                    tttACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCAAGGc                                                                                            >  2:852848/1‑239 (MQ=255)

                                                                                                                                                     ttcccc‑AGTAAAATATTAGTCGCCCGAAAAAACTCGGAAAG‑GGGA‑T‑AAAATGGCCCA‑G‑TTCCCGCGAGATC‑AGAGTGGTGGCCAGTGCCGCAATCACCATTGGGAACAGCATTGAGGATAACACAACCAGAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGGCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                           <  2:584727/234‑1 (MQ=255)

                                                                                                                                                     ttACGC‑AGAAAAATAAAAGTTCCCAAAAAAAAAGCGAAAAC‑TGGT‑A‑AAAAGGGACCA‑TTTTCCCGCGA‑AAA‑GGGGCGAGGGCCAGTTCCGAAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATGCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                           <  2:1095816/239‑1 (MQ=255)

                                                                                                                                                      ttccgacgaaaaCTAAAAGTTCCCAAAAAATTAGCG‑AAAA‑TGGT‑A‑GAAAAGGACCC‑GTTGCCCAGGA‑AAATTGAGTGGGGGCACGTCCCGAAATATACTTGGGCAAAAGAAGGAAAGCTAAACCAACCTCAATACGGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                           <  2:343395/230‑1 (MQ=255)

                                                                                                                                                      tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATaa                                                                                                                                                              >  1:399578/1‑172 (MQ=255)

                                                                                                                                                      tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGGGTTTTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCaa                                                                                          >  2:583842/1‑238 (MQ=255)

                                                                                                                                                              tAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTa                                                                                    >  1:824892/1‑238 (MQ=255)

                                                                                                                                                              tAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGAAAAACA‑GGCACAGTGTTGTCCGGGTTTAAAGAGGAAAAACAAAGATCTGGCCAGGCAGAACCTaa                                                                                   >  2:682756/1‑238 (MQ=255)

                                                                                                                                                              tAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCCGGACACAACCTGCCAGATACACATGGCA‑AATGTTATACGGGTTTATAGAGGAAAAACCAAATTTATGCCAAGAAGAACCTaa                                                                                   >  2:812197/1‑238 (MQ=255)

                                                                                                                                                                   tAAAAGTTG‑CCAAAAAAAAACTCAAACTTGGT‑A‑AAAAGAGCCTA‑GTTCCCCGAGA‑ATA‑TGGCTTGGGCCCTGTCCCCAAATATACATGGGGGAGAGCATGGAGGTAAAACAACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                              <  2:824892/239‑1 (MQ=255)

                                                                                                                                                                   aaaagGTTGCCAAAAAAAAATCGCAAAG‑TGTT‑T‑AAAAGAGACCA‑GTTCCCCGCAA‑ATA‑TGAGCGGGACCCATTTCCGCAATATACATTGGGGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                              <  1:682756/238‑1 (MQ=255)

                                                                                                                                                                           gCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCT‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAATTAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGCGTTGTCCGGGTTTATCGAGGTAAAACCAAGTTCCGGCCAGGCCGAAACTACACTAGAAATaaaa                                                                      >  1:986106/1‑239 (MQ=255)

                                                                                                                                                                            cccGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAGTAACAAATTc                                                                                                                                                                        >  2:55092/1‑141 (MQ=255)

                                                                                                                                                                            cccGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAGTAACAAATTc                                                                                                                                                                        <  1:55092/141‑1 (MQ=255)

                                                                                                                                                                            cccGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGGTTATCGAGGGAAAACCAAGTTATGCCCAGGCAAAAACAAAACaaaaaaaaaaaa                                                                     >  2:1166159/1‑239 (MQ=255)

                                                                                                                                                                             ccaaaaaTAATCGAAACC‑GGGT‑T‑AAAAGGGACCA‑GTTTCCGGGGA‑ATA‑GGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                                                    <  1:1034161/236‑1 (MQ=255)

                                                                                                                                                                                  aataccctcAAAC‑T‑GT‑T‑AAAAGAAAACAGGTGCCCCGGAA‑ATA‑GGAGCTGGAGCCGGTCCCGAAATATCCATTGGGGAGAAAATTGGGGATAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                               <  1:1186289/231‑1 (MQ=255)

                                                                                                                                                                                  aataatCGCAAAC‑GGGT‑T‑AAAAGAAACCC‑TTTTCCCGAGA‑ATA‑TGAGCTGGACCCAGTTCCGCAATAAACATTGGGGAGAGCATTGAGGCTAAACCAACGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                               <  1:583842/239‑1 (MQ=255)

                                                                                                                                                                                      atCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCAGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACAAAACCTGCCAGCTAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTCATGGCCAGGCAGAACCTAACCCAGAAATAAAAAACaagcaaac                                                           >  2:920094/1‑232 (MQ=255)

                                                                                                                                                                                          cccaaatagggttaaagataaCCA‑GTTCCCCGGAA‑ATT‑TGGGCTGGAGCCAGTGCCGAAATAGACATTGGGGAAGCCATTGAGGCAAACCCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagca                                                          <  1:1202582/219‑1 (MQ=255)

                                                                                                                                                                                                  gT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                >  1:1240322/1‑159 (MQ=255)

                                                                                                                                                                                                  gT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGATCTGGCCAGGCAGAACCTACACCAGAAAAAAAAAAAAGAACAAAAGCAAACTTaa                                                >  2:34034/1‑239 (MQ=255)

                                                                                                                                                                                                     taaaaaGAGACCG‑TTTCCCCGGAA‑ATG‑TGAGCTGGCGCCAGTTCCGAAATATAAGTTGGCGAGAGCATTGAGGAAAACCCAACTCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                <  1:1287603/236‑1 (MQ=255)

                                                                                                                                                                                                              aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGTCCAGGCAGAACCTACCCCAGCAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCacc                                      >  2:1325881/1‑238 (MQ=255)

                                                                                                                                                                                                              aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaa                                                                      >  1:1210079/1‑207 (MQ=255)

                                                                                                                                                                                                              aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGAAGCAACCTTAAGGGAATCacg                                      >  1:526394/1‑239 (MQ=255)

                                                                                                                                                                                                                          ccggaaattggggggggACCCAGTCCCGAAATATACAGTGGCGGAAGCATGGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAACCTTAAGTGAATCACGACGACCGaa                             <  1:34034/224‑1 (MQ=255)

                                                                                                                                                                                                                               ata‑gGAGGTGGAGCCAGTTCCGCAATTACCATTGGCGAGAGCATTGAGGCTAAACCAACCACAACACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                        <  1:125352/238‑1 (MQ=255)

                                                                                                                                                                                                                               aattggggctggacaacgtgccacAATATACATTGGCGAAAGAATGGAGGACAAACCACCCCAAACGCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                        <  2:349861/216‑1 (MQ=255)

                                                                                                                                                                                                                                          gAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAAACTTAAGAGAATCACGACCACCAAAACGGTAAcagaaaaaa             >  2:125352/1‑231 (MQ=255)

                                                                                                                                                                                                                                                      cAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGAAATCTTAAGTGAATAACGACGACCGAACCGGTTACTGCAATAACAACCCAGGGc  >  1:236233/1‑238 (MQ=255)

CCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGC  >  NC_000913/4241562‑4242037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`