Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,361,543 A→T 11.6% D131E (GAT→GAA)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,361,5430AT11.6% 229.6 / 3.5 86D131E (GAT→GAA) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (60/14);  new base T (2/9);  total (62/24)
Fisher's exact test for biased strand distribution p-value = 7.58e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGAT  >  NC_000913/4361309‑4361776
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          
gTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCtt                                                                                                                                                                                                                                        >  1:1100135/1‑238 (MQ=255)
   aCAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCCGGTGGCGTTGCGGGGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCtgtg                                                                                                                                                                                                                                      >  1:841727/1‑238 (MQ=255)
   aCAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGGGTTTTG‑AATTT‑TAA‑AACT‑GTGGGGTGGGGAATCTTTgg                                                                                                                                                                                                                                     >  2:972652/1‑239 (MQ=255)
     aaCTTTTTTTTACCATATTAATGTCAATATCGCGTGGATTGAAGAAAATACGCGATTTGCTCATAGGAAGGCGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCa                                                                                                                                                                                                                                   <  2:1100135/239‑1 (MQ=255)
           tatttaCCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGAGGGGTTTTG‑AATGT‑TAA‑GACT‑GGGGGAGGGGGATTTCGGGGCAGGAA‑cc                                                                                                                                                                                                                            >  1:508662/1‑239 (MQ=255)
           tatttaCCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGAAACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCGGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑cc                                                                                                                                                                                                                            >  1:853202/1‑239 (MQ=255)
                            tCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGGGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GGGGGAGGGAGAATCTGTGGAAGGAA‑ACGCCCCTGGTAAAggggg                                                                                                                                                                                                           >  2:782302/1‑239 (MQ=255)
                              aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAACACCATACCCAAAAGG‑TAATGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGTTGCCGTTGCGGAGTTTTG‑AATGT‑TAA‑GACT‑GTGGTAGGGAGAATTTGGGGCAGGAA‑AAGCCCCTGGGATAGGGGGaa                                                                                                                                                                                                         >  2:71712/1‑238 (MQ=255)
                              aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAACACACTACCCAATAGG‑TAGTGAATCGGTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGAGGATGCGGTGATTTG‑AATTC‑TAG‑GACT‑GTGGGAGGGAGATTCTGTGGTAGGCA‑ACGcccttgtgaagaggggag                                                                                                                                                                                                         >  2:170130/1‑223 (MQ=255)
                              aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACTCAAAAGG‑CAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAG‑GACT‑GTGGGAGGGGGAATCTGTGGCTGGGA‑CCGCCTCTGGCATAGGGGGAg                                                                                                                                                                                                         >  1:625738/1‑239 (MQ=255)
                              aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAg                                                                                                                                                                                                         >  1:628191/1‑239 (MQ=255)
                              aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCCCTGGTTTAGGGGGAg                                                                                                                                                                                                         >  1:1127635/1‑239 (MQ=255)
                              aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGGGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAg                                                                                                                                                                                                         >  1:1255119/1‑239 (MQ=255)
                                tatCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGc                                                                                                                                                                                                       >  1:666580/1‑239 (MQ=255)
                                     cgTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGa                                                                                                                                                                                                  >  1:175288/1‑239 (MQ=255)
                                     cgTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTAAAgg                                                                                                                                                                                                              >  1:781055/1‑227 (MQ=255)
                                     cgTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGACGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGACTCTGGTAATGGGGGAGGCGAAGa                                                                                                                                                                                                  >  1:1328817/1‑239 (MQ=255)
                                          aTTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑CAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGGGGTTTG‑AATGT‑TAA‑CACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CGCCCTCGGTGCAAGGGGGAgggggaaagagca                                                                                                                                                                                             >  2:1376848/1‑228 (MQ=255)
                                              aGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAgatgtctcttttacacatttccg                                                                                                                                                                                         >  1:264618/1‑217 (MQ=255)
                                                   aaTACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGGAAAGGGGGGGGGGAAAAAAGAATTATTTcc                                                                                                                                                                                     >  2:716612/1‑238 (MQ=255)
                                                   aaTACGCGATTTCCTAAAAGGAAGGCTTGTATAAAGACTTAAAAACGCAACCAGTGCTACCCAGATACCAAACGACAACAGGAAAAAAAACCATCCCAAAAGGG‑TAGTAATTCGTTTG‑CTTTTAAC‑TGGG‑GATTGTCAAGGGGGTGTTGGGGTGTTTTG‑AATGT‑AAA‑ACCT‑GGGGGAGGGAAAATCTGGGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCct                                                                                                                                                                                    <  1:782302/239‑1 (MQ=255)
                                                    aTACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGTCGTTGGGGTGTTTTG‑AATGT‑TAA‑GACT‑CGGGGAGGGAGAATCTGTGGCAGGAC‑ACGCCCCTGGGGAAGGGGGAGGGGGAGAGAGAATTAtttgcc                                                                                                                                                                                    >  2:938218/1‑235 (MQ=255)
                                                                cTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAgg                                                                                                                                                                                                        >  1:479160/1‑206 (MQ=255)
                                                                           aCGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGTTAGCATTATTTCCTCTCCCCCTTCTTCGTCGTACCAgc                                                                                                                                                            >  1:182247/1‑238 (MQ=255)
                                                                             gtgtATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATa                                                                                                                                                          >  1:974358/1‑239 (MQ=255)
                                                                             gtgtATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCCCTTCTTCGCTGtcacaaaaa                                                                                                                                                          >  2:960970/1‑231 (MQ=255)
                                                                                     cacctgaaaaaCCCCACCAGTCCAACACAGATCCCTAAAGACAACAGGAAAAAAACCCATCCCAAAAGGG‑TAGTGAATCGTTTG‑TTTTGAGC‑TGGG‑GATTGTTCGGGTGGGTTTGCGGTGTTTGA‑AATTT‑AAA‑GGCT‑GTGGGAGGAAGATTCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCAc                                                                                                                                                  <  2:797372/232‑1 (MQ=255)
                                                                                       aCTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCa                                                                                                                                                >  1:1179033/1‑239 (MQ=255)
                                                                                        ctaaaaacCGCCACCAGTGCTAACAAGATACATAACGACAACAAGAAAAAAAACCATCCCAAAAAGG‑TAGTGAATCTTTTG‑CTTTTAAC‑GGGG‑TATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GGGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCAt                                                                                                                                               <  1:960970/235‑1 (MQ=255)
                                                                                           gAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGAACCAGATAACCCGCACCCTTaa                                                                                                                                            >  1:137906/1‑239 (MQ=255)
                                                                                                   tACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGGTAACCGGCACCATTAATTtatttg                                                                                                                                    >  1:797372/1‑236 (MQ=255)
                                                                                                     cctgttcTACAAAGATCCATAAGAAAAAAAGAAAAAAAACCCATCCCCAAAGGG‑TAGTAATTCTTTTG‑TTTTAAAC‑GGGG‑GTTTGTTGGGGGGGCGTGGGGGTGTTTTA‑AATGT‑TAA‑GACT‑GGGGGAGGGAAAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCcg                                                                                                                                  <  1:524719/233‑1 (MQ=255)
                                                                                                     caagggcacacaaaatcccaaaCGAAAACAGAAAAAAAACCCATCCCAAAAGGG‑TAGGTATTCGTTTG‑TTTTTACC‑GGGG‑GATTGTCCGGGTGGCGTTGGGTTGTTTTG‑AATGT‑TAA‑GACT‑GGGGGAGGGAAATTCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCcg                                                                                                                                  <  1:716612/219‑1 (MQ=255)
                                                                                                             acacAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGGGGGGAAGATAGCATTATTTCCCCTCCCCCTTTTTCCCTGTTACAAAAAAACGGCACCATTAATTTATAACCCCCCTTTgg                                                                                                                          >  2:45896/1‑239 (MQ=255)
                                                                                                             acacAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTTTTCGCTGTTCCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTgg                                                                                                                          >  1:689205/1‑239 (MQ=255)
                                                                                                             acacAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCCAATAACCGGCACCATTAATTTATAGCCGCGCttttg                                                                                                                          >  1:124773/1‑237 (MQ=255)
                                                                                                               tggggtgatattaagagagagaaaaaaaaaCCCTAACAAAAAGT‑GTGGGAAATGTTTG‑GTTTTTAC‑GGGGTGATGGCTAGGTTGGCGTGGGGGTGTTTGC‑AAGTT‑GAA‑GAGG‑GTGGGAGGGAGATTCTGTGGCAGAAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTgg                                                                                                                          <  2:917612/216‑1 (MQ=255)
                                                                                                               acaGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGGATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTAACAGATAACCGGCACCATTAATTTATAGCAGCGCTTTggca                                                                                                                        >  1:578414/1‑237 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGGA‑CCGCCTCTGGGATAGGGGGAGGGGCAGATAGCCTTATTTTCTCTCCCTCTTCTTCCCTGTACCAGGTAACCGGCACCCTTCATTTATAACCCCCCTTTgggtccgt                                                                                                                    >  2:941470/1‑233 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAgg                                                                                                                                                                                                              >  1:376276/1‑149 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGTTAGAATTATTTCCTCTCCCTCTTCTTCGCTGGACCAGATAACCGGCCCCCTTAATTTAAAGCCGTGCTTTGGTACAGt                                                                                                                    >  1:1235343/1‑239 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCCTTAAt                                                                                                                                           >  1:1202353/1‑216 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTTTAACCCCGCTTTGGTACAGt                                                                                                                    >  1:413195/1‑239 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTCCAGt                                                                                                                    >  1:795106/1‑239 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGt                                                                                                                    >  1:727155/1‑239 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGt                                                                                                                    >  1:975707/1‑239 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCCCTTCTTCGCTGTACCa                                                                                                                                                              >  1:365255/1‑197 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGGATAGGGGGAGGCGAAGGAAGCATTATTTCCTCTCCCCCTTCTTCGCTTTACCAGATAACCGGCCCCATTAATTTTTAACCCCGCTTTGGTAAAGt                                                                                                                    >  2:965934/1‑239 (MQ=255)
                                                                                                                   aTACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTATTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCCCTCCCTCTTCTTCGCTGTACCAGAAAAACGGCACACTTAATTTATACCCGCCCTTTGGTAAAga                                                                                                                    >  2:521331/1‑238 (MQ=255)
                                                                                                                    tcctaaacgaaaaaaaaaaaaaaacCCAAACCAAAAAGG‑TTG‑GTATGTGTTG‑CTTTTAACGGGGG‑GTTTGTTCGGGGGGGGTTGGGGTTTTATG‑AATTT‑TAA‑GGCG‑GGGGGAGGGAAAATCTGTGGCAGAAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTa                                                                                                                   <  2:974358/222‑1 (MQ=255)
                                                                                                                     acccacaccgaaaaaaaaaaaaaaCCCAAACCAAAAGGTTTGTG‑ATGGTTTGTCTTTTAAG‑GGGG‑GTTTGTTGAGGTGGGGTGGGGTTGTTTTG‑AATGT‑GAA‑GGGT‑GGGGGGGGGAAATTCGGGGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTa                                                                                                                   <  2:1179033/223‑1 (MQ=255)
                                                                                                                     aCCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGGAGATAGAATTATTTCCTCTCCCCCTTCTTCGCTCTACCCGACAACCGGACCCCTTAAATTAACACCCCCCTTTGGTaaaaaac                                                                                                                  >  2:631582/1‑233 (MQ=255)
                                                                                                                        tAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGCGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCCCCATTACTTTATAGCCCCGCTTTGGTAACGAAGCGa                                                                                                               >  2:262747/1‑239 (MQ=255)
                                                                                                                        tAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGAGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGCAATGGGGGAGGCGAAGATAGCATTATTTCCGCTCCCTCTTCTTCGCTGTACCAGCAAACCGGCAACATTAATTTTTACCCGCGCCTTGGTAAAGTAGCGa                                                                                                               >  2:603019/1‑239 (MQ=255)
                                                                                                                         aaCGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGGGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAGGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTTCCAGATAACCGGAACCAGTAATTTATAACCGCCCTTTGGTACAGTAGCggt                                                                                                              >  1:37483/1‑237 (MQ=255)
                                                                                                                           cGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCGGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCCTTCTTTCCTCTCCCTCTTCTTCGCTGTACAAGAAAACCGGCACCATTAATTTAAAGCCGCGCTTTGGTACAGGAGCGaaaa                                                                                                            >  2:675905/1‑236 (MQ=255)
                                                                                                                           cGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGGATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTTGCTGGACCAGATAAACGGCCCCCTTAATTTTTAGCCCCGCCTTTGGACAATAACGaaaa                                                                                                            >  2:98132/1‑236 (MQ=255)
                                                                                                                               aacaGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTTGCTGTTCCAGGTAACCGGCACCATTAATTTTTAg                                                                                                                                     >  1:151200/1‑210 (MQ=255)
                                                                                                                                    gAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGCAGATAGCCTTATTTCCTCTCCCTCTTCTTCGCCGTACCAGATAACCGGCACCCTTAATTTATAACCCCCCTTTGGTACCGTAACGACAAACACAGGAc                                                                                                   >  2:50969/1‑239 (MQ=255)
                                                                                                                                     aaaaaaaaCCAATCCAAAAGGG‑TTGGGAATGTTTTG‑TTTTTAAT‑GGGG‑GTTTGTTCGGGGGGGGTGGCGGTTTTTTG‑AGTGT‑TAG‑GCCG‑GGGGGAGGGAAAATTTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGa                                                                                                    <  1:521331/237‑1 (MQ=255)
                                                                                                                                      aaaaaaaCCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAAt                                                                                                                                                                                                                                           >  1:213806/1‑102 (MQ=255)
                                                                                                                                      aaaaaaaCCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAAt                                                                                                                                                                                                                                           <  2:213806/102‑1 (MQ=255)
                                                                                                                                              cATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTAGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCCGTTTGGTACAGTAGCg                                                                                                                >  1:917612/1‑216 (MQ=255)
                                                                                                                                                tACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGGAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCCACATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGTCAGGCCTATCttattcct                                                                                       >  1:300503/1‑233 (MQ=255)
                                                                                                                                                            taggaattcttttG‑CTTTAAAC‑GGGG‑GTTTGTGCAGGGGGCGTGGCGTTTTTTTA‑AATGT‑AAA‑GGTG‑GGGGGAGGGAAAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTa                                                                              <  1:45896/227‑1 (MQ=255)
                                                                                                                                                             ggTGAAGGGTTGG‑TTTTAAAC‑TGGG‑GTTTTGTCGGGTGGCTGTGCGGTTTTTTG‑AATGT‑TAA‑GACT‑GGGGGAGGGAGAATTGGTGGCAGGAC‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAAt                                                                            <  1:50969/237‑1 (MQ=255)
                                                                                                                                                              gTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATg                                                                           >  1:846471/1‑238 (MQ=255)
                                                                                                                                                              gTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGa                                                                          >  1:323536/1‑239 (MQ=255)
                                                                                                                                                              gTGAATCGCTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGAc                                                                                                        >  1:1193119/1‑209 (MQ=255)
                                                                                                                                                                    ggTTGG‑CTTTAAAG‑GGGG‑GTTTGTTCAGGTGGCGTGGGGGT‑TTTTG‑AATGT‑GTA‑GGCTGGGGGGAGGGAAATTCGGGGGAGGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACg                                                                    <  1:288805/238‑1 (MQ=255)
                                                                                                                                                                      tttG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGACCCATTAATTTATAACCGCGCTTTGGTACAGTAGCGAAATAAACCGGACATTCTTTATCATTATCTTTTAATGAGTTatcta                                                                  >  2:256796/1‑235 (MQ=255)
                                                                                                                                                                       ttG‑CTTTTAAC‑TGGG‑TATTGTGCAGGGGGCTTGGCTGTTTTTGA‑AATTT‑TAA‑GACT‑GGGGGAGGGAGAATTGGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAg                                                                 <  1:941470/239‑1 (MQ=255)
                                                                                                                                                                                      gggatttGTGCGGGTGGTGGTGTGGTTTTTTG‑AATGT‑AAA‑AGGT‑GGGGGAGGGAAATTGGGGGGCGGAAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATa                                                       <  1:539789/232‑1 (MQ=255)
                                                                                                                                                                                      gg‑gATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATAc                                                      >  1:570403/1‑237 (MQ=255)
                                                                                                                                                                                               tggggggtggttgggtttttttG‑TATTT‑TAG‑GGTT‑GGGGGGGGGAGTATTTGTGGCAGGAC‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTcac                                            <  2:655455/221‑1 (MQ=255)
                                                                                                                                                                                                 gggggggtgtggggTTTTGTG‑AATTG‑TAA‑GGGT‑GGGGGGGGGATATGCTGGGGGAGGAC‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACaa                                          <  2:795106/227‑1 (MQ=255)
                                                                                                                                                                                                        tgtggggTTTTTTG‑AATTTGTAA‑GGGT‑GGGGGGGGGAGTCTCGTTGGGAGAAA‑CCGGCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGa                                     <  2:975707/233‑1 (MQ=255)
                                                                                                                                                                                                        gTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAAACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGAAACTCTGCGTCACAACGTGAtt                                   >  1:655455/1‑239 (MQ=255)
                                                                                                                                                                                                        gTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATAAAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTtagaaaactctgttccaaacgtgatt                                   >  2:848878/1‑217 (MQ=255)
                                                                                                                                                                                                          ttggtgtttttGAAATGT‑TAA‑GTGG‑GGGGGGGGGAAAATTTGGGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGAtt                                   <  1:256796/231‑1 (MQ=255)
                                                                                                                                                                                                          tgtgggtttttG‑AAAGT‑TAAGGTTT‑GGGGGAGGGATAAGTTGGGGAGGGAC‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGAtt                                   <  1:972652/231‑1 (MQ=255)
                                                                                                                                                                                                              gtttttgG‑AATGT‑TAA‑GGCG‑GGGGGAGGGAAATTCTGGGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGa                              <  1:965934/235‑1 (MQ=255)
                                                                                                                                                                                                                                 tggt‑gtgGGGGGGGGAATTTGGGGGAAGAACCCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCa              <  2:1304074/236‑1 (MQ=255)
                                                                                                                                                                                                                                       tgGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGa                                                                                                         >  1:226343/1‑142 (MQ=255)
                                                                                                                                                                                                                                       tgGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACAtt        >  1:1304074/1‑239 (MQ=255)
                                                                                                                                                                                                                                             ggagATTCTGGGGAATGCC‑CCTCCTTTGTTATAGGGTGGGACGAAGATCTCATTATTTCCTCTCACTATTCTTCGTTTTACCAGATAACCTGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTTTCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGAt  <  2:1089126/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          
GTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGG‑TAGTGAATCGTTTG‑CTTTTAAC‑TGGG‑GATTGTTCAGGTGGCGTTGCGGTGTTTTG‑AATGT‑TAA‑GACT‑GTGGGAGGGAGAATCTGTGGCAGGAA‑CCGCCTCTGGTATAGGGGGAGGCGAAGATAGCATTATTTCCTCTCCCTCTTCTTCGCTGTACCAGATAACCGGCACCATTAATTTATAGCCGCGCTTTGGTACAGTAGCGATATAGACAGGACTATCTTCATCATTATCTTTTAATGACTTACGTAGTTCTGAGATACTCTGCGTCACAACGTGATTGGTGACAATACTTCTCTTCCAGACATTATCGAT  >  NC_000913/4361309‑4361776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑