Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,458,425	A→C	20.9%	T156P (ACC→CCC)	pmbA →	putative antibiotic peptide MccB17 maturation peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,458,425	0	A	C	20.9%	166.5 / 8.8	91	T156P (ACC→CCC)	pmbA	putative antibiotic peptide MccB17 maturation peptidase
Reads supporting (aligned to +/- strand):  ref base A (45/24);  new base C (3/19);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 2.27e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

TAGCGCATCATCCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGT  >  NC_000913/4458190‑4458658                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gggcctagcctcCCCCGATTTTAGCCCGCAGTCATTTGCCCGGACTGTACAGGCGGCGCTGGATATTGCCGTGTAGTCCCCCCCAGATCCCTGTGCCGGGGTGG‑CAGACAAAGAGCTGCTGGCCTTGGACGCACCAGATCT‑‑CGACTTGTTCCCCCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑c                                                                                                                                                                                                                                               <  2:374567/227‑1 (MQ=255)          atcCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAAATCCCTGGGCCGGCGGGG‑CAGAACCAAAGCTGCTGGGCCTTGAAGCGCCCGACTC‑‑CGACTTGTTCCACCCCCCGCCAAGTTCCCC‑GGG‑TGAAGCCCTTGAACCGGCGGCCCGGCCAGAAAA‑GGCGGCATGT‑CAGGGCGGA‑AAAAACA‑CCA‑CC‑CAAA‑CCGAAg                                                                                                                                                                                                                                  >  2:1305969/1‑239 (MQ=255)          atcCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCCCCAGATCCCTGTGCCGGCGTGG‑CCGACAAAGAGCTGCTGGCCTTTGCCGCACCAGCTCT‑‑CGGCTTGTTCCACCCTGCGGAAGTTTCCCC‑GGG‑TGAAGCCCTTTAACAGGCGGCCCGCGCAGAAAA‑GGCGGCACTG‑CG‑GGGGGCGCAACACA‑ACA‑CC‑AAAA‑CCAAAg                                                                                                                                                                                                                                  >  2:100042/1‑239 (MQ=255)             cACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTAAAGGCGGGGCTGGATATTGCCCGTTAGTCCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGGAAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑g                                                                                                                                                                                                                              <  1:382425/239‑1 (MQ=255)                           cGCAGGCCATGGCCCGTACTGTACGGGCGGCGCTGGATATTGCCCGTTATCCCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCC‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGc                                                                                                                                                                                                                <  1:968650/239‑1 (MQ=255)                                 ccATTGCCCGGACTGTACAGGCGGCGCTGGATATTGCCCGTTATCCCTCGCCAGATCCCTGGGCCGGCGTGG‑CAGACAAAGAGCTGCGGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑a                                                                                                                                                                                                          <  1:100042/238‑1 (MQ=255)                                  cATTGCCCGTACTGTACAGGCGGCGCGGGATATTGCCCGTTATCCCTCGCCAGATCCCTGGGCCGGCGTGG‑CAGACAAAGGGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCCCCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACg                                                                                                                                                                                                        <  1:808761/239‑1 (MQ=255)                                  agaagcgggtccTGTACGGGCGGCGCTGGATATTGCCCGTTATCCCTCGCCAGACCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACg                                                                                                                                                                                                        <  1:1065427/228‑1 (MQ=255)                                            aCTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGGCAAACGCA‑TTA‑CC‑AAAA‑CCGGAGGG‑GGAAGCTTTAACAGCCACC‑ACCGTGCCAAAgg                                                                                                                                                                                              >  1:1183209/1‑238 (MQ=255)                                                tACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCCTTT‑CA‑GGCGGACAAAACCA‑TCC‑CC‑AAAA‑CCCAAAGA‑GGGAGCTTTAAAAACCACA‑ACgcggtgaaaagtttt                                                                                                                                                                                          >  2:947897/1‑225 (MQ=255)                                                     gcggcgCTGGATATTGCCCGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCCCGCCGAACA‑AGCGGCATTT‑CA‑GGCGGACAAAAGCC‑ACA‑CC‑AATA‑CCGAAAGA‑GGGAACCTTAAAACCCACC‑ACAGCGGCaaaaa                                                                                                                                                                                              >  2:1065427/1‑228 (MQ=255)                                                      ggcgttgggtttgtGCCTGTTATCCCCCCCCAGGCCCCTGGGCCGGGGGGG‑CAAACAAAGGGCTGTGGGCCTTGGCCCCCCCAGTACG‑‑CGACTTTCCCCCCCCTGGGGAGTTTTCCCC‑GGA‑TGAGGCCATTGAACGGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAACCGCA‑TCA‑CC‑AATC‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAAc                                                                                                                                                                                    <  2:219678/226‑1 (MQ=255)                                                          gcTGGATATTGCCCGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGGTTCCCC‑GGA‑TGAAGCCATTTAACTGGCGGCCCGCGCCGAACA‑CGCGGCATTG‑CA‑GGCGGGCAAACCCC‑TCC‑CC‑AACACCCGAAGTG‑GGCGCTTTTAAAACCCCCA‑AGGGTGCAAAATTTTTTGCCAACaca                                                                                                                                                                                 >  2:467301/1‑237 (MQ=255)                                                             ggtgtttGCCCTTTATCCCCCACCA‑ACCCCCGTGGCGGGGGGGCCAGAAAAAGCGTTGCGGGCTTTGGACGCACGAGACCC‑‑CGACTGTTGCCCCCCGGCGGACGTTTCCCC‑GGA‑TGAAGCCATTGACCGGGCGGCCCGCGCAGAACG‑GGCGGCTTTG‑CC‑GGCGGAAAACCGAA‑TCC‑CC‑AATA‑CCGAAGGT‑GGCGGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACg                                                                                                                                                                             <  2:1338362/234‑1 (MQ=255)                                                                tttttccctttttcccTCCCCAGCTCCCTGGGCGGGGGTGG‑CAAACAAAGGGCGTCGGGCCTTTGACGCCCCAGATCC‑‑CGACTTGTCCCACCCGGCGGAAGTTTCCCG‑GGA‑GGAAGCCATGGACCGGGCGGCCCGCGCAGAACA‑GGCGGCTTTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGG‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGc                                                                                                                                                                           <  2:729744/224‑1 (MQ=255)                                                                     cccGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCAACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCCCT‑ACGGTGTCAAAGTTTTTGGCAACCGCCACGGCATGtt                                                                                                                                                                      >  1:862187/1‑238 (MQ=255)                                                                        gTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑CATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAg                                                                                                                                                                 >  1:1298183/1‑239 (MQ=255)                                                                         ttATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACCAACGCA‑TCA‑CC‑AATA‑CCGAAGGG‑GGCAGCTTTAAAAGCCACT‑ACGGTGTCAAAGATTTTGGCACAAGCCACGGCATGTT‑TCAgg                                                                                                                                                                >  1:23384/1‑239 (MQ=255)                                                                         ttATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAAACCC‑TCA‑CC‑AATA‑CCGAAGGG‑GGCAGCGTTAACAGCCACT‑ACGGTGGTAAAGATTTTGGCAACAGGCACGGCATggtcaaag                                                                                                                                                                 >  1:22509/1‑232 (MQ=255)                                                                          tataCCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTTGCCTTTGACGCACCAGATCT‑‑CGGCTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCCTTGAACTGCCGGCCCGCGCAGAACA‑AGCGGCATTG‑CA‑GGCGGACAAACGCC‑GCA‑CC‑AATA‑CCCAAAGT‑GGCGGGATTAACAGCCAATCAGGGGGTAAAAG‑TTTTGGCaaaaacaacgcacaggtagcagg                                                                                                                                                                >  2:450654/1‑218 (MQ=255)                                                                           atccccagccggcccccGGTGCGGGGGTGG‑AAGACAAGAGGCTGCGGGCTTTTGACGACCCAGACCC‑‑GGATGTCTCCCCCCGGGAGGAAGTTCCCCG‑GAA‑GGAAGCCATTGAACGGGCGGCCCGCGCAAAACG‑GGCGTCATTG‑CA‑GGCGAACAAACGCA‑TCC‑CC‑AATA‑TCGAAGGG‑GGCAGCTTAAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGt                                                                                                                                                              <  2:554399/225‑1 (MQ=255)                                                                                  gCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGCAAAACCCC‑CCA‑CC‑AATA‑CCGAAGGA‑GGCAGCTTTAAAAACCACC‑ACGGTAGCAAAGTTTTTGGCAAAAGCCCCGGCATGTTCGCA‑GGTTACttc                                                                                                                                                        >  2:27960/1‑236 (MQ=255)                                                                                  gCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGG‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACACCCACGGCAGGTT‑TCACGGTTACTGcc                                                                                                                                                       >  1:1277690/1‑238 (MQ=255)                                                                                   ccagtccccTGTCCCGGCTTGC‑CAGAAAAAGGGCTGCGGCTCTTGGACGCACCAGATCC‑‑CGCTTTGTCCCCCCCGGCGGAAGTTCCCCG‑GGA‑GAAACCCATTGAACTGGCGGCCCGCGCAAAACG‑GGCGGATTTC‑CA‑GGGGGACAACCGCA‑TCC‑CA‑AATA‑CCGAAGTG‑GGCAGTTTAAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTc                                                                                                                                                      <  2:621164/233‑1 (MQ=255)                                                                                      gATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACAGCCACGGCCAGTT‑GCAGGGGTACGGCCCAAc                                                                                                                                                   >  1:387858/1‑239 (MQ=255)                                                                                              tgCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCCCT‑ACGGGGTCAAAGTTTTTGGCCACAGCCACGGCATCTT‑GCAGGGTAACTCCt                                                                                                                                                       >  1:1225021/1‑227 (MQ=255)                                                                                              tgCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCCAAGTTTTTGGCAACAGCCCCGGCATGTT‑GCAGGGGTACTGCTCAACCCGtatt                                                                                                                                            >  1:78163/1‑235 (MQ=255)                                                                                              ggCCGGCGGGG‑CAGACAAAGAGCTGCTGGCCTTGGACCCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCAtt                                                                                                                                           <  1:366156/238‑1 (MQ=255)                                                                                              gcccggtctGG‑CAAAAAGAGGGCTGCT‑GCCTTTG‑CGCGCCCGATCTTCCGACTTGTCCCACCCGGGGGAATTTTCCCG‑GGA‑TAAAGCCATTAACCGGGCGGCCCGCGAAAAACG‑GGCGCCTTGG‑CA‑GGCGAAAAAACGCA‑CCC‑CC‑AATA‑CGGAAGGT‑GCAAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCAtt                                                                                                                                           <  2:599486/231‑1 (MQ=255)                                                                                               ggccggctttcaggcaaaaggtctgctgCCTTTTGCCGCATCAGGTCT‑‑GGATTTGTCCCCCCTGGGGGAATTTTCCCC‑GGA‑TGAGGCAGTTAACCGGGGGGCCCGGGAAGAACA‑GGCGGACTTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAGGGG‑GGACGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCAtt                                                                                                                                           <  2:72385/217‑1 (MQ=255)                                                                                               gCCGGCGTGG‑CAGACAAAGAGCTGCTGCCCTTTGACGCCCCAGATCC‑‑CGACTTGTCCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCAtt                                                                                                                                           <  1:947897/238‑1 (MQ=255)                                                                                                   gCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCCTTG‑CA‑GGCGGACAAAAGCA‑TCA‑AC‑AAAA‑CCGAAGGG‑GGCCGCTTTGAAACCCACT‑ACGGGGTGAAAATTTTTGGGAACACGCAAGGGCTGTT‑GCAAGGTTACTGCCCAACGGGCCATTCGcc                                                                                                                                       >  2:1071287/1‑237 (MQ=255)                                                                                                   gCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGct                                                                                                                                       >  1:326979/1‑238 (MQ=255)                                                                                                   gCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑CCA‑CC‑AATA‑CCGAAAGG‑GGCAGCTTTAAAAGCCACC‑AAGGGGTCCAAGTTTTTGGCAACAACCCAGGCCAGGT‑GCCGGGGTTCAGCCCAAAGCGGCATTcccc                                                                                                                                       >  2:768470/1‑235 (MQ=255)                                                                                                    cGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGGGTCATTCGccc                                                                                                                                      >  1:864944/1‑236 (MQ=255)                                                                                                    cGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACCCC‑TCC‑CC‑AATA‑CCCAAGGT‑GGCAGCTTTAACAGCCCCT‑ACGGTGTCAAAGTTTTTTGCAACAACCACGGCCAGTT‑GCAGGGGTACTGCTCAACACGTCAATcctcc                                                                                                                                      >  2:628481/1‑234 (MQ=255)                                                                                                                       gctgGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCCGCTGTGTAATTTCCg                                                                                                                   >  1:870985/1‑239 (MQ=255)                                                                                                                        ctgGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGCCCAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCCAT‑CCGGGGTCAAAGTTTTTGGCAACAGCCACGGCCTGTT‑GCCGGGTTACTGCTCAACGCGTCATTTGCTCTCCCGCTGTGTAATTGccca                                                                                                                  >  2:789421/1‑237 (MQ=255)                                                                                                                         tgGCTTTTGCCCCACTAGATCT‑‑CGCCTTGCTCC‑CCCCGGGGGAGTTTCCCCGGGA‑GGAAGCCGTTGAGCGGGCGGCCCGCGAAGAACG‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑CCA‑CA‑AATC‑CCGAAGGT‑GGCAGTTTAAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCgaa                                                                                                                 <  2:387858/239‑1 (MQ=255)                                                                                                                              tttGACGCACCAGATCC‑‑CGACTTG‑TCCCCCCCGGGGGAGTTTTCCC‑GGAGTGAAGCCATTGACCTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTAAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGaaaa                                                                                                            <  1:789421/239‑1 (MQ=255)                                                                                                                                tGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGG‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACAGCCCCCGCAGTTT‑GCAGGGGTACTGCCAAACGCGTCATTTCCCCTCCACCTGTGGAATTGCCGGAGaaaaag                                                                                                          >  2:841631/1‑237 (MQ=255)                                                                                                                                    gCACCAGATCT‑‑CGACTTGTTCCCCCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCg                                                                                                       <  1:1071287/238‑1 (MQ=255)                                                                                                                                      aCCAGCTCC‑‑CGACTTGTTCCCCCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATC‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGaaa                                                                                                    <  1:522466/239‑3 (MQ=255)                                                                                                                                      aCCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCACACCGTCATTCGCGCTCCAGCTGTGTAATTGCCGAGGAAAATGGCGaca                                                                                                    >  1:1285730/1‑237 (MQ=255)                                                                                                                                      aCCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGATTTTGGCAACAGCCAAGGCATGTT‑GCAGGGGTACCGCTCAACGCGTCATTTGCTCCCCAGCCGGGGAATTGCCGAAAAAAAAgggcaaa                                                                                                    >  2:645203/1‑234 (MQ=255)                                                                                                                                      aCCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAAAAGCCACT‑AAGGGGGCAAAGTTTTTGGCAACAGCCCCGGGTTGTT‑GCAGGGGTACTGCCCACCCCGTCATTCCATCACCCCCCGTGTAATTGACGAAGaaaaaaggggta                                                                                                    >  2:1121591/1‑231 (MQ=255)                                                                                                                                       ccAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTTCCGAAGAAAATGGCGatat                                                                                                   >  1:879439/1‑239 (MQ=255)                                                                                                                                       ccAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGatat                                                                                                   >  1:754288/1‑239 (MQ=255)                                                                                                                                       ccAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTTTAATTGCCGAAGAAAATGGCGatat                                                                                                   >  1:751043/1‑239 (MQ=255)                                                                                                                                              tcccgctttttcccACCCGGCGGAAGTTCCCCC‑GGA‑TGAACCCATTGAACTGGCGGCCCGGCGAGAACG‑GGCGGCATTG‑CA‑GGGGGACAAACGCA‑TCC‑CC‑AATC‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGc                                                                                              <  1:1019611/227‑1 (MQ=255)                                                                                                                                               tcccgcTTGTCCCCCCCCGGGGGAATTTCCCCGGGA‑GGCACCCATTAAAGCGGGCGCCCGCG‑AAAACAGGGGGGAATTG‑AA‑GGCGAACAACCGCA‑TAC‑CC‑AATA‑CGGAGGGG‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGc                                                                                              <  2:805964/234‑1 (MQ=255)                                                                                                                                               cgcctcTTGTTCCCCCTGGGGGAGGTTTCCCG‑GGG‑TGAGGCAATTGAACTGGCGCCCCGCGCAAAAGG‑GGCGG‑ATGGACA‑GCGGAAAAAACTCT‑ACC‑CC‑AATC‑CCGAAGGT‑GGCAGCTTAAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCAGTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCg                                                                                             <  2:19438/234‑1 (MQ=255)                                                                                                                                                   ccttgtccccccggggggAGGTTCCCCG‑GGA‑GGAACCCATGGAACGGCCGACCCGCGC‑GAACA‑GGGCGGAATG‑CC‑GGGCGAAAAAAGCATTCA‑CA‑AATC‑CGAAAGGT‑GGCAGCTTAACCAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAt                                                                                         <  2:751043/223‑1 (MQ=255)                                                                                                                                                   atttGTCCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAGGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGGGGCATTG‑CA‑GGAGGACAAACGCA‑TCA‑CC‑AATC‑CCGAAGGT‑GGCAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAt                                                                                         <  1:841631/237‑1 (MQ=255)                                                                                                                                                    cTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCCGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCACAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGATCaa                                                                                                                                                    >  1:1210829/1‑179 (MQ=255)                                                                                                                                                       gTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGGCAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTATTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGGTTCATTCGCTCTCCCGCGTGGTAATTGCCGAAAAAAAAGGCAAAATGGAGGGGGACTAc                                                                                      >  2:1186404/1‑238 (MQ=255)                                                                                                                                                       gTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGCCAAACGCA‑TCA‑CC‑AATA‑CCGAAGGG‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTACACGCGTCCTTCGCTCTCCAGCCGGGTACTTGCCCAACACAAAGGGGCTATGGAGCGTGATAAc                                                                                      >  2:101107/1‑238 (MQ=255)                                                                                                                                                           cccccgggggagttctCCCG‑GGG‑TGAACCATTTAAACGGGCGGCCCGGGCAAAACG‑GGCGCCATTG‑CA‑GGCGGAAAACCGCA‑CCC‑CA‑AATC‑CCGAAGGT‑GGAAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTa                                                                                 <  2:864944/224‑1 (MQ=255)                                                                                                                                                             cccTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCCCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTCCGCCTac                                                                                >  1:1279204/1‑238 (MQ=255)                                                                                                                                                                 gCGGAAGTTCCCCC‑GGA‑TGAAGCCATTGACCTGGCGGCCCGCGGAGAACA‑GGCGGAATTG‑CA‑GGCGGACAAACGCA‑TCC‑CA‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCAt                                                                           <  1:628481/239‑1 (MQ=255)                                                                                                                                                                  cGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTa                                                                                                                                                            >  1:387577/1‑157 (MQ=255)                                                                                                                                                                  cGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGCTATGGAGCGTGATTACGCCTAAACCAtt                                                                          >  1:863513/1‑239 (MQ=255)                                                                                                                                                                        ttccccc‑GGT‑TGAGCCCATTGAGGGGGCGGCCGCCAGCGAAGA‑GGGGGTATTG‑CA‑GGCGGACAAACGCA‑ACC‑CC‑AATG‑CCGAAGGT‑GGCAGTTTAACCAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCCAGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTTGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGt                                                                    <  2:544437/236‑1 (MQ=255)                                                                                                                                                                         tccccc‑GGA‑TGAAGCCATTGACCTGGCGCCCGGCGAAGAACA‑GGCGGCATTG‑CA‑GGCGGAAAACCGCA‑ACC‑CC‑AATA‑CCGAGGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGt                                                                    <  1:506005/236‑1 (MQ=255)                                                                                                                                                                          tCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCACCAGCCCCGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCACCTGTGTAATTGCCGAAGAAAAAGGGGATATGGAGCGGGATTTCGCCTACAACAtttggcctgc                                                                  >  2:1096891/1‑231 (MQ=255)                                                                                                                                                                          tCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTAATGCTCACAGCGTCATTCGCTCTCCAGCTTGGTAATTGCCGAAAAAAAAGGGGAAATGGGGGGGGATTAACCCCACACCATggggggggg                                                                  >  2:719325/1‑233 (MQ=255)                                                                                                                                                                            ccc‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACG‑GGCGGCATTG‑CA‑GGCGGACAACCGCA‑TCA‑CA‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGa                                                                <  1:645203/239‑1 (MQ=255)                                                                                                                                                                                 gggtGAAGCCATGAAACTGGCGGCCCGCGAAAAACA‑GGGGGCATTG‑CA‑GGGGGAAAAACGCA‑TCC‑AC‑AATT‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGa                                                             <  1:569664/236‑1 (MQ=255)                                                                                                                                                                                       gcccATTGAACTGGGGGCCCGCGCAGAACG‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGAt                                                        <  1:1032787/236‑1 (MQ=255)                                                                                                                                                                                        gCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAAGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCCGGGTAATTGCCGAAGAAAATGGCGATATGGGGCGTGATTACCCCTACACCATTGGTCGTGCCAtagggga                                                         >  2:341168/1‑230 (MQ=255)                                                                                                                                                                                           aTTGACCGGGCGGCCCCGGCAGACAG‑GGGGGCATTG‑CA‑GGCGGACAAACGCA‑GCA‑CC‑AATC‑CCGAAGGG‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCa                                                   <  1:768470/239‑1 (MQ=255)                                                                                                                                                                                           aTTGAACTGGCGGCCCGCGCAGAACG‑GGCGGCATGG‑CA‑GGCGGAAAAAC‑CT‑TCC‑CCAAATC‑CCGAAGGT‑GGCAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCa                                                   <  1:339155/239‑1 (MQ=255)                                                                                                                                                                                             tGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGGTACTGCCCAACGCGTCATTCCCTCTCCAGCCGGGTAATTGCCGAAGAAAAAGGGGATATGGAGCGGGGTTACGCCTCAACCCTTGGtggggtgggaacggatgggcaaa                                                 >  2:1164824/1‑217 (MQ=255)                                                                                                                                                                                             tGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGA‑GGCAGCTTTAACAGCCACT‑ACGGTGTCCAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACCCGTCATTCGCTCTCCCGCTCTGTAATTGCCGAAGAAAATGGCGTTATGGAGCGTGATTTCGCCTACACCATTGGTCGGGCAAGAAGGGAGCTGCaaa                                                 >  2:204818/1‑239 (MQ=255)                                                                                                                                                                                              gAACGGGGGCCCGGCGCAGAACA‑GGCGGCATTG‑CG‑GGCGGACAAACGCT‑TCA‑CC‑AATG‑CCGAAGGT‑GGCCGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAAc                                                <  1:412942/239‑1 (MQ=255)                                                                                                                                                                                                    gcggcCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGGT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCACCTGTGTAATTGCCGAAGAAAAGGGCGAGATGGAGCGTGATTACGCCTCACCCCTTGGGCGTGggaggacggagcgtccaacgccaaa                                          >  2:1193495/1‑220 (MQ=255)                                                                                                                                                                                                      ggcCGGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCC‑CC‑AATT‑CCGAAGGT‑GGCAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCagag                                         <  1:341168/238‑1 (MQ=255)                                                                                                                                                                                                         ccGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATAAGGGATCTGCAAACGCCagatgggg                                     >  1:1015538/1‑234 (MQ=255)                                                                                                                                                                                                         ccGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGGGTCAAAGTTTTTGGCAACAGCCACGGCCTGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAAAGGCGATATGGAGCGTGATTCAGCCTACACCATTGGCCGTGCGATGAGCGATCTCCAAACGCAAGAGgggg                                     >  2:33142/1‑239 (MQ=255)                                                                                                                                                                                                         ccGCGCAAAACG‑GGTCGCATTG‑AA‑GGGGGA‑AAACGCT‑ACACCC‑AATG‑CGGAGGGT‑GCCACTTTCAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGGT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTggg                                     <  2:326979/239‑1 (MQ=255)                                                                                                                                                                                                                   acgggcggcATTG‑CA‑GGCGGACAACCCAA‑TCA‑CC‑AATA‑CGGAGGGT‑GGCAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCg                            <  1:1209340/236‑1 (MQ=255)                                                                                                                                                                                                                         ggcATTG‑CG‑GCGGGAAAAACGCA‑TCA‑CA‑AATG‑CCGAGGGT‑GGCGCCTTTACCAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACtgt                       <  1:462736/238‑1 (MQ=255)                                                                                                                                                                                                                            aTTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCCTTGGTCGTGCGATGAGCGATCTCCAAACGCCCGAGTGGGTTGGGGGCGACTGTGGTc                   >  1:165320/1‑239 (MQ=255)                                                                                                                                                                                                                            aTTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGCCttggct                    >  1:411793/1‑233 (MQ=255)                                                                                                                                                                                                                            aTTG‑CA‑GGCGAACAAACGCA‑TCC‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTACCAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCt                    <  1:12459/238‑1 (MQ=255)                                                                                                                                                                                                                              tG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGGGTAATTGCCGAAGAAAATGGCGATATGGAGCGGGATTACGCCTACACCATTGGTCGTGGGATGAGCGATATCGAAACGCCAGAGTTGGTTGGGGCCGACTGTGCTccc                 >  2:516108/1‑237 (MQ=255)                                                                                                                                                                                                                                tggcgggcgcaaaaCGCA‑CCC‑CC‑AATT‑GCGGAGGTGGGCAGCTTTACCAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCg                  <  2:1015538/228‑1 (MQ=255)                                                                                                                                                                                                                                cggcgggcgcaaaacccaaccA‑CC‑AATC‑CGGAGGGG‑GGCATCTTAACCAGCCACT‑ACGGTTTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCg                  <  2:879439/219‑1 (MQ=255)                                                                                                                                                                                                                                       ggACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGACGTGCGATGGGCGATCTGCAAACGCCAGAGCGGGTGGGGGCCGATTGGTCTCGCCGGACtt          >  2:967520/1‑239 (MQ=255)                                                                                                                                                                                                                                           aaaCGCA‑TCC‑CA‑AAGC‑CCGAAGGT‑GGCAGTTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATc      <  1:1096891/239‑1 (MQ=255)                                                                                                                                                                                                                                               gta‑TCC‑CC‑AAGA‑CCGAAGGT‑GACAGCTTTAACAGCCCGT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGt  <  1:1104115/237‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                TAGCGCATCATCCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGATCCCTGTGCCGGCGTGG‑CAGACAAAGAGCTGCTGGCCTTTGACGCACCAGATCT‑‑CGACTTGTTCCACCCTGCGGAAGTTTCCCC‑GGA‑TGAAGCCATTGAACTGGCGGCCCGCGCAGAACA‑GGCGGCATTG‑CA‑GGCGGACAAACGCA‑TCA‑CC‑AATA‑CCGAAGGT‑GGCAGCTTTAACAGCCACT‑ACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTT‑GCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGT  >  NC_000913/4458190‑4458658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑