BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,463,875	T→G	11.4%	H279P (CAC→CCC)	treC ←	trehalose‑6‑P hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,463,875	0	T	G	11.4%	427.9 / 2.8	156	H279P (CAC→CCC)	treC	trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand): ref base T (48/82); new base G (22/3); total (71/85)
Fisher's exact test for biased strand distribution p-value = 3.27e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

TTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACC  >  NC_000913/4463642‑4464108

ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGACCAATGTTTTCAAAGCCGCAAAGCCAGGTTTAGCCCACGTCCCTTTTTAACCA‑CCGGGCGAATCGACCTTCAGGtgagtg                                                                                                                                                                                                                                        >  1:198454/1‑236 (MQ=255)

   ccTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAAAACCAGGTTTAGCCAGCGCCCATTTTTTACCA‑CCGGGGTAATCGACCTTCAGGGGATGaaaa                                                                                                                                                                                                                                     >  1:527887/1‑239 (MQ=255)

       ccttcccagccaccccATCCGCAGCATTTTTGCCGGAGGCACGGGTTATTCCCCTTCATCCCCAAAGCGGAAAACAATGCGCGGCTGATTGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCTACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTaa                                                                                                                                                                                                                                 <  2:527887/225‑1 (MQ=255)

                 ccgacgaccGCCAGCAGTTTTGCCGACGGCCCGAGGTATTCACCTTCATCACCAAAGGGAGAAACAATGCGCGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGa                                                                                                                                                                                                                      <  1:1080181/231‑1 (MQ=255)

                 agcctcATCGGCAGCATTTTTGGCGCAAGGACACAGTATTCGCCTACATCACCAAAACGAGAAACAATGCGCTGCTGATCGATGTTACACCAGTACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGACAGAGGCGGAACAATGTTTTCAACGCCACAAAGGCAGGTTTAGCCTGCGGCCATTTTTCACCA‑CCGGGATAATCGTCCTTCAGGTGATGAAAATTAAAG‑GTCATCg                                                                                                                                                                                                                       <  1:56815/233‑1 (MQ=255)

                   caCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑ACGGCATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACa                                                                                                                                                                                                                    >  1:266678/1‑239 (MQ=255)

                   caCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTAGCGGAACAATGTTTTTAACCCCCCAAAGTCAGGTTTAGCCCGCGTACAATTTTCACCA‑CCAGGATAAAAGACCCTCCAAGGTTGAAAATTaaaagaaatcagca                                                                                                                                                                                                                     >  2:153654/1‑228 (MQ=255)

                    cccATCGGCAGCATTTTTGCCGCAGGCACGGGGTATTCCCCTTCATCACCAAAGCGAGAAACAATTCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGATACGTTGTGAATTCCTTGATGCCAGTGGCGGAACAATGTTTACAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGa                                                                                                                                                                                                                      <  2:337296/235‑1 (MQ=255)

                    cccATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGa                                                                                                                                                                                                                      <  1:735683/235‑1 (MQ=255)

                      cATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCAAGCGTCCCTTTTTCACCA‑CCGGGATAATCGACCTTCAGGGGATGAAAATTAAAG‑GTCCTCGCCAAt                                                                                                                                                                                                                  >  1:838922/1‑238 (MQ=255)

                       aTCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCA‑CCGGGGTAATCGACCTTCAGGGGGTGAAAATTAAAG‑GCAATCGGCAAttt                                                                                                                                                                                                                >  1:72207/1‑238 (MQ=255)

                        tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAAAAAAGTTTTCAACGCCCAAAAGTCACGTTTAGACAGCGTCCCATTTTTACCA‑CCCGGCTAACAGACCCTCAGTTGGGAAAAATTAAAG‑Gttcacgcaaattc                                                                                                                                                                                                                >  2:1057736/1‑226 (MQ=255)

                           ccAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTTAACACCACACAGCCCGGTTTTGCCATCGTCCCTTTTTTCCCA‑CCCGGATAAACTCCCTTCCGCGGATGAAAAATAAAgacaacagccaaaaaacac                                                                                                                                                                                                             >  2:24721/1‑221 (MQ=255)

                            cAGCATTTTGGCCGCAGGCACGCGGTATTCCCCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTg                                                                                                                                                                                                            <  1:887745/238‑1 (MQ=255)

                             aGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAAAAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCCGCGTCCATTTTTCACCA‑CCGGGGTAATCGACCTTCAGGTGATGAAAATTAAAA‑GTCATCGACAATTCACTGc                                                                                                                                                                                                           >  1:1147871/1‑238 (MQ=255)

                             aGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGAGGCGGGACAATGTTTTCTACGCCACCAAGCCAGGTTTATCCATCATCCCTTTTTCACCA‑CCGCGTTAAACGACCTCCCGGGGATGAAAATTaaaaggaatcccaaatcaaccccc                                                                                                                                                                                                           >  2:370646/1‑218 (MQ=255)

                                            ggCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCAGGATAATAGACCTTCAGGTGATGAAAATTAAAT‑GACATCCACAATTCACTGCCTGTCAGAGCCCCGTa                                                                                                                                                                                           >  1:335966/1‑239 (MQ=255)

                                                     tATTCACCTTCATAAACAAAGCGGGAAACAATGGGCGGCTGATCGGGGTTACCACAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTaa                                                                                                                                                                                                                                 <  1:1307518/193‑1 (MQ=255)

                                                      ttttcccttcctacaccaaGCGAGAAACAAGGCGCGGCTGATTGGGGTTACACCGTAACAAGGCATTCCATGCCACGTTGTGCATTCCTTGTTGGCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                  <  2:66061/221‑1 (MQ=255)

                                                      aTTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                  <  1:370646/238‑1 (MQ=255)

                                                              tcatcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                  <  1:700429/230‑1 (MQ=255)

                                                               catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAAAGTCCGGGTTTGCCCCCGTCCCTTTTTTACCC‑CCCGGGTAAACGACCTTCCGGTGATGAAAATTAAAA‑GGACTACCCAATTCAATGCCCTTCACAGCcgcggaaacggaggaaatccacaag                                                                                                                                                                        >  2:76787/1‑218 (MQ=255)

                                                               catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCCGGTTTAGCCAGGGTCCATTTTTCACCA‑CCGGGGTAATAGACCTTCAGGTGATGAAAATTAAAG‑GTCCACGACAATTCACTGCCCACCAAGGCCCCGTAACGCTGGCaaatcgcaaa                                                                                                                                                                         >  2:228554/1‑230 (MQ=255)

                                                               catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGGGTCCATTTTTTACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAT‑GTCATCGACAATTCAATGCCTGTCAGAGCCCCGTATCGCTGGCAATGCTaaaa                                                                                                                                                                         >  1:1163929/1‑237 (MQ=255)

                                                               catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGCCAATTCACTTCCTTTCAGAGCCGCGCTTTGCTGGGCATGCTCAAgg                                                                                                                                                                        >  1:564172/1‑239 (MQ=255)

                                                               catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAAAGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCC‑CCGGGATAATCGACCTTCAGGTGATGAACATTAAAG‑GTCATCGACAATTCACTTCCTGTCAGAACCCCGGTTTGCTGGCCATtctccaag                                                                                                                                                                        >  1:245814/1‑235 (MQ=255)

                                                                 tcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAAAGTTTTTAACGCCACAAAGTCAGGTTTAGCCACCGTCCATTTTTCACCA‑CCGGGGTAATCGACCTTTAGGGGATGACAATTAAAA‑GGGATCGCCAATTTATGCCCTGGCAGAGCCGGGTagtccatgcaacgcccaag                                                                                                                                                                         >  1:809001/1‑218 (MQ=255)

                                                                     caaagcagaaaaCAAGGCCCGGGTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGGCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCtggtg                                                                                                                                                                  <  2:1147871/230‑1 (MQ=255)

                                                                     cAAAGCGAGAAACAATGCGCGGCTGATCGGTGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                  <  1:468272/223‑1 (MQ=255)

                                                                        aGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAAAGTCAGGTTTAGCCAGCGGCCCTTTTTCACCA‑CCGGGATAAACGACCTTCCGGTGATGAAAATTAAAG‑GTAATCGACAATTCCCTCCCTGTCAGACCCGCGTATCCCTCGCaaagtccaaagcccttacct                                                                                                                                                               >  2:700429/1‑221 (MQ=255)

                                                                        aGCGAAAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTggag                                                                                                                                                               <  1:979254/239‑1 (MQ=255)

                                                                          cGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAAAGTTTTCAACGCCACAAAGGCCGGTTTTACCCGCGGCCCTTTTTTACCC‑CCGGGGAAATAGACCTTCAGGGGCTGGAAATTAAAG‑GGAATCGACAATTCCCTCCCTCTCAGAGCCCCTGAACCCTGGCAAACCTCAAgagtggggggggg                                                                                                                                                             >  2:56646/1‑227 (MQ=255)

                                                                           gagaAACAATGCGCGGCTTATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTggagga                                                                                                                                                             <  1:768229/238‑1 (MQ=255)

                                                                                     gggtggttgttggTGGTTCCCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTa                                                                                                                                                  <  2:838922/227‑1 (MQ=255)

                                                                                      gctggttgttCGTGTTTAACCCAAAAAAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTAc                                                                                                                                                 <  2:72207/230‑1 (MQ=255)

                                                                                       gcgGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTAc                                                                                                                                                 <  1:620667/238‑1 (MQ=255)

                                                                                          gCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCCAGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGGGCTGAAAATTAAAG‑GTCATTGACAATTCAAATCCTTTCATAGCCGCGAATTGCTGGCAATGCTTAAGGCTGGTGGAGGAGAATTCACCTACgg                                                                                                                                               >  1:1299519/1‑237 (MQ=255)

                                                                                           cTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACCCCCCCAAGTCAGGTTTAGCCCGCGTCCCTTTTTCACCA‑CCCGGATAATCGACCCTCACGGGGTGGAAATTAAAG‑GTCCTCCCCCATTTCAGCCCCGTCCAAGCCGCGCACCCCTGGCAACGCCCAACGCGGGGGGGGGACACTTCAccccagggca                                                                                                                                            >  2:203945/1‑231 (MQ=255)

                                                                                              aTCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGCCAATTCACTGCCTGCCAGAGCCCCGTATCCCTGGCAATGCTCAAGGCTGGTGGGGGACATTTCCCCTACGGGCC‑TTa                                                                                                                                        >  1:563533/1‑239 (MQ=255)

                                                                                                    gtgacacAATAACAAGGCATTCAATGCTACGTTGTGCATTCCTGGTTGACAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTGTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCAACGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCcgc                                                                                                                                  <  2:1193078/236‑1 (MQ=255)

                                                                                                    gTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGa                                                                                                                                                                                                                      <  1:231885/156‑1 (MQ=255)

                                                                                                    gTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTAc                                                                                                                                                 <  1:26626/225‑1 (MQ=255)

                                                                                                     ttACCCCAGAACAAGGCATTCCATGCTTCGTTGTGCATTCCTTGTTGCCAGTGGCCGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCcgc                                                                                                                                  <  1:1168478/238‑1 (MQ=255)

                                                                                                     ttACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGGCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCcgc                                                                                                                                  <  1:153654/238‑1 (MQ=255)

                                                                                                      ttccccAGAACAAGGCATTCCATGCTACGTTTTGCATTCCTTGTTGCCTGTGGCGGAACAATGTTTTCAACCGCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGt                                                                                                                                <  2:328951/235‑1 (MQ=255)

                                                                                                      tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGt                                                                                                                                <  1:230266/239‑1 (MQ=255)

                                                                                                      tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCCGGTGGAGGACATTTCACCTACGGTCA‑TTAACCcgcg                                                                                                                                 <  1:943699/238‑1 (MQ=255)

                                                                                                        cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GCCATCGACAATTCACTGCCTGTCAGAGCCCCGTATCGCTGGCAATGATCACGGCTGGTGGAGGGCATTTCACCTACGGTCA‑TTAACCCGCGTgc                                                                                                                              >  1:720992/1‑238 (MQ=255)

                                                                                                         acCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGa                                                                                                                                                                                                                      <  1:935271/151‑1 (MQ=255)

                                                                                                                   ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCACAAGTAAGGTTTAGCCAGCGGCCATTTTTCACCC‑ACGGGATAATCGACCTTCAGGTGGTGAAAATTAAAG‑GTAATCGACAATTCCCTg                                                                                                                                                                                                            >  2:935271/1‑151 (MQ=255)

                                                                                                                    ggaTTCCATTCTAGGGTGTGCATTCCTTGTTGCCAGGGGCGGAACAATGTTTTCAACGCCAGAAAGTGAGGTTTAGCCAGCCTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGAAAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATc                                                                                                                  <  2:1299519/237‑1 (MQ=255)

                                                                                                                     ccttCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGGCCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACAt                                                                                                                   <  1:421639/235‑1 (MQ=255)

                                                                                                                     cATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATc                                                                                                                  <  1:76787/238‑1 (MQ=255)

                                                                                                                       ttCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATcgc                                                                                                                <  1:532621/238‑1 (MQ=255)

                                                                                                                           aTGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGGGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAAAGCCGCGGTTCGCTGGCAATGCTCAAGGCTGGTGGCGGACATTTAACCTACGGTCA‑TTAACCCCCGTGGCGTAAACCCATTCCGGTTc                                                                                                           >  1:225015/1‑239 (MQ=255)

                                                                                                                            tGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATc                                                                                                                  <  1:1214355/231‑1 (MQ=255)

                                                                                                                                   ttGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCagag                                                                                                                                                                                                  <  1:930855/145‑1 (MQ=255)

                                                                                                                                      tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAAAGTCAGGGTTAGCCCGCGTCCATTTTTTACCA‑CCGGGATAATCGACCTTCAGGTGATGGAAATTAAAG‑GTCCTCGACAATTCACTGCCTGTCAGAGCCGCTGATCGCTGGGCAAGCTCACGGCTGGGGGGGGGACTTTCACCCACGGTCATTTAACCCCCG‑GGGGGAAAAACAACCCGGTTCATTTCCGGCaa                                                                                                >  2:899446/1‑239 (MQ=255)

                                                                                                                                      tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCCATGCTCCAGGCTGGTGGGGGACATTTCACCTACCGTCA‑TTAACCCCCGGGGCGGAAACAAACCGCGGTTCATCCCGTGCaa                                                                                                >  1:1359781/1‑239 (MQ=255)

                                                                                                                                      tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGGGGAGGACATTTCACCTACGGTCA‑TTAACCCCCGGGGCGTAAACACACCGCGGTTCATCTCGTGCaa                                                                                                >  1:367274/1‑239 (MQ=255)

                                                                                                                                      tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAAAGCCCCAAAGTCAGGTTTTGCCAGCGGCCATTTTTTACCA‑CCGGGATAATCGACCTTCAGGGGGTGAAAATTAAAG‑GTAATCGACAATTCACTGCCCTTCACGACCCCGTACTGCTGGCCATCCTCACGGCTGGAGGGGGACACTTCCCCCCCCGCAA‑TAAACCCCCCCGGCGAAAACACAtacagtgtactctacgcaca                                                                                                >  2:460075/1‑220 (MQ=255)

                                                                                                                                        atttCCTTGTTTCCTGTGGCGGAAAATGGGTTTCAACGGCACAAATGCAGGGTTAGCCAGCCACCATTTTTCACCG‑ACGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCaaaa                                                                                              <  2:55718/237‑1 (MQ=255)

                                                                                                                                           tCCTTGTTGCCAGTGGCGGAACAATGGTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCCTCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGGGGGGGACATTTTACCTACGGCCA‑TTAAACCGCCTGGCGTTAACACATCCCGGGTCATCTCTGGCCAAAAc                                                                                            >  1:237502/1‑238 (MQ=255)

                                                                                                                                               tggtgacaagggCGGAACCATGTTTCAAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCgtg                                                                                       <  1:1200307/229‑1 (MQ=255)

                                                                                                                                                  tgCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGGTCATCTCTGGCCAAAAATCgtggg                                                                                     >  1:105338/1‑236 (MQ=255)

                                                                                                                                                       gTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTTTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCCCCTACGGTCA‑TTAACCCGCTTGGCGTAAACACATCCCGGGTCATCTCGGGCAAAAACTCGCGTGCTCGtt                                                                               >  1:175215/1‑238 (MQ=255)

                                                                                                                                                       gTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCCCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGGCA‑TTAACCCGCGTGGCGTAAACACATCCCGGGTCATTCCGGGCAAAAAACCGTGTGGCCGTg                                                                               >  1:1074991/1‑239 (MQ=255)

                                                                                                                                                         ggCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGGCCATTTTTTACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCCGTCAGAGCCCCGTATCGCTTGCAATGCTCAAGGCTGGTGGGGGGCCTTTCACCCTCGGTCA‑GTAACCCCCGGGGCGGAAACACATCCCGGGTTCTCTCCTGGAAAAAACCCTGGGCTCGGGGc                                                                             >  2:389639/1‑239 (MQ=255)

                                                                                                                                                             gAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGC‑AAAACTCGTGTGCTCGTGGCCCGt                                                                         <  1:699783/238‑1 (MQ=255)

                                                                                                                                                              aaCAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  1:3076/238‑1 (MQ=255)

                                                                                                                                                               aCAATGTTTTAAACGCCACAAAGTCAGGTTTAGCCATCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTc                                                                        <  1:1068564/238‑1 (MQ=255)

                                                                                                                                                                  aTGTTTTCAACTCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAACCACATCGCGGTTCATCTCGTGCAAAAACTCGTGTACCCGTGGCCCGTCGgtg                                                                    >  1:133502/1‑239 (MQ=255)

                                                                                                                                                                        tCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTAc                                                                                                                                                 <  2:106634/157‑1 (MQ=255)

                                                                                                                                                                                     ggcAGGTTTAACCAGCGTCCATTTTGCACCC‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                  <  1:1275995/236‑1 (MQ=255)

                                                                                                                                                                                      tCAGGTTTAGCCCGGGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGGGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                  <  1:889586/237‑1 (MQ=255)

                                                                                                                                                                                           tttAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCAACGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTc                                             <  1:1075386/237‑1 (MQ=255)

                                                                                                                                                                                            ttacacAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAg                                          <  1:1069250/234‑1 (MQ=255)

                                                                                                                                                                                            ttAGCCTGGGTCCATTTTTCCCCC‑CCGGTATAAACGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAg                                          <  2:266678/239‑1 (MQ=255)

                                                                                                                                                                                            ttAGCCCGTGTCTTTTTTTCCCCA‑CCGGGATAATCGACCTTCGGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAg                                          <  2:225015/239‑1 (MQ=255)

                                                                                                                                                                                               gCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCCCGGTTCCTCTCGTGCAAAAACTCGGGTGCCCGGGGCCCGTCGGGGAAAAAACGCAGCCCGTGGCCGTCCCggc                                        >  2:1275995/1‑237 (MQ=255)

                                                                                                                                                                                                ccAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGGGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGCCGCCCGTCGCCTGCCAggac                                       >  1:914149/1‑236 (MQ=255)

                                                                                                                                                                                                    cGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCACAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCCCCGCCCAggcactcag                                  >  1:456935/1‑232 (MQ=255)

                                                                                                                                                                                                      tCCCTTTTTCCCCA‑CCTGGATAACCCACCGTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCCCGTCCTGTCAGAGCCGCGTATCGGTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAggg                                <  2:1152339/239‑1 (MQ=255)

                                                                                                                                                                                                            tttCCCCACCCGGGATAATCGACCTGCAGGTGATGAAAAGTAAAA‑GCACACGACAATTCACTGCCTGGCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAAcg                           <  1:646193/239‑1 (MQ=255)

                                                                                                                                                                                                              tcccca‑ccGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCAGCGACAATTCACTGCCTGGCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCgg                        <  2:564172/236‑1 (MQ=255)

                                                                                                                                                                                                                acca‑ccGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCAtc                                                                                                        <  2:869358/157‑1 (MQ=255)

                                                                                                                                                                                                                acca‑ccGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCAtc                                                                                                        >  1:869358/1‑157 (MQ=255)

                                                                                                                                                                                                                      cGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGCCTTCAGGGAAACACGGGGCTTTg                 >  1:1037248/1‑239 (MQ=255)

                                                                                                                                                                                                                      cGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGCCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAACCGCGGGCCTTTg                 >  1:510726/1‑239 (MQ=255)

                                                                                                                                                                                                                          aTAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag              <  1:389639/238‑1 (MQ=255)

                                                                                                                                                                                                                           tAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGt                      <  1:1091506/229‑1 (MQ=255)

                                                                                                                                                                                                                             aTCGACCTTCAGGTGGTGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTTTGCTCGTGGCCCGTCGGGGTAGAACCGACGCCCGCCGCCGCCCCGGTCTTCAGGGAAACGCGGGCCTTTGgaaacaa          >  2:78332/1‑234 (MQ=255)

                                                                                                                                                                                                                             aTCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGCGCGGGTAGAAGCGACGCCCGTCCCCGCCCCGGCCTTCAGGGAAACGCGGGGCTTTgggagacc          >  2:817592/1‑233 (MQ=255)

                                                                                                                                                                                                                               cgtccTTCAGTTATTGAAAATTAAAGTGTC‑TGCACAAGTCCCTGCCGGTCAGAGCCGCGTACCGCTGTCAATGCTCAAGGCTGGTGGAGGACATTTCCACTACGGTCA‑TTAACCCGCGTGGCGTGAACCCATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGa        <  2:1122/236‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGCCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTc     >  1:875134/1‑238 (MQ=255)

                                                                                                                                                                                                                                   cTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTAGGTGTAGAATCGACGCCCGTCGCCGCCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGCGCAGATTc     >  1:1347400/1‑238 (MQ=255)

                                                                                                                                                                                                                                   cTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGGCGGTGGAGAATCGACGCCCGTCGCCGTCCAGGTCTTTAGGGAAACACGGGTcctgtcctctcaacaaa     >  2:297798/1‑222 (MQ=255)

                                                                                                                                                                                                                                   cTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGAGTGCTCGTGGCCCGTCGGGGTAAAACCACCGCCCGTAGCCGTCCAGGCCTTCAGGGAAACACGGGGCTTTGGAATTCGGAtta     >  1:289417/1‑237 (MQ=255)

                                                                                                                                                                                                                                     tCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGGTCATCTCGTGCAAAAACTCGTGTTCTCGTGGCCCGCAGGTGTGGAAGCGACGCCAGTCGCCGTCCAGGGCTTCAGGGAACCGCGGGTCTTTGGAGATcctgtcccct  >  2:1091506/1‑230 (MQ=255)

                                                                                                                                                                                                                                     tCAGGTGAATAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGGGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTcacc  <  1:822528/239‑1 (MQ=255)

TTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCA‑CCGGGATAATCGACCTTCAGGTGATGAAAATTAAAG‑GTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCA‑TTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACC  >  NC_000913/4463642‑4464108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`