BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,481,717	T→G	16.3%	T708P (ACC→CCC)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,481,717	0	T	G	16.3%	239.5 / 6.6	101	T708P (ACC→CCC)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (38/42); new base G (14/6); total (53/48)
Fisher's exact test for biased strand distribution p-value = 8.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GATGCATCGTACTGCGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAG  >  NC_000913/4481479‑4481952

gaTGCATCGTACTGCGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGt                                                                                                                                                                                                                                                >  1:967324/1‑239 (MQ=255)

    cATCGTACTGCGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATCCCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGaa                                                                                                                                                                                                                                             <  1:1178665/238‑1 (MQ=255)

       cGTGCTGCGGGAACGGCTGCAGCAGGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTTCAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑a                                                                                                                                                                                                                                      <  1:944047/239‑1 (MQ=255)

       cGTACTGCGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑a                                                                                                                                                                                                                                      <  1:319597/239‑1 (MQ=255)

       cGTACTGCGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGATGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTAAAGTCACCAGCGAATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGGCAGCTCGAGCTACCAGTCACAGAACGGGTTTCCGGGGaagcct                                                                                                                                                                                                                                         >  1:112118/1‑235 (MQ=255)

         tACTGCGGGAACGGCTGCAGCTGTAGGGTGTCGGCGGGGATTCCGCAAAGTTCGTTCACCCGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATa                                                                                                                                                                                                                                    <  2:111461/239‑1 (MQ=255)

              cGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCCCGCGGAACCAGTCACAGAACTGGTTCCCGGGGGA‑CT‑C‑ATCCCGaa                                                                                                                                                                                                                               >  1:573483/1‑239 (MQ=255)

              cGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGAATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCCGCCCGAGATTCCCGTTCCGAAATCGGTTCCAGGGGAA‑CT‑ccacacgaa                                                                                                                                                                                                                                >  1:655082/1‑231 (MQ=255)

                             caTGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGAGCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATa                                                                                                                                                                                                                                    <  1:949232/219‑1 (MQ=255)

                              atgatgGTGTCGGCAGTGATCCCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATc                                                                                                                                                                                                               <  1:948786/239‑1 (MQ=255)

                               tgatgGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTTGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGGCAGCTCGAGATACCCGGCACAGAACTGGTTCCCGGTGAA‑CC‑C‑ATACCGAATCCCTCCGGCGAAATCg                                                                                                                                                                                                              >  1:268877/1‑239 (MQ=255)

                                     gggcGGCAGTGATACCGCAAAGTACTGTCCCAGGGTGCCAGATGGTTTCGGGGA‑TAAACGGAATGATCGGATGCGCGA‑GCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACATAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCgg                                                                                                                                                                                                        <  1:594820/235‑1 (MQ=255)

                                       tCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGGCCGCTCGAGGTACCAGGTCCAGAACTGGTTCCAGGGGAA‑CT‑C‑ATACAGAAGGCCTGCGGCGATATTGAAGCGGaa                                                                                                                                                                                                      >  1:1308403/1‑239 (MQ=255)

                                         ggCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAg                                                                                                                                                                                                     <  1:276038/238‑1 (MQ=255)

                                            aGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTTGGCAGCCCGAGGTACCCGTCACAGAACTGGTTCCCCGCGGA‑CT‑C‑ATACAGACAGCCTAGGGCGaataccaacgggaaaaag                                                                                                                                                                                                  >  2:1306612/1‑221 (MQ=255)

                                                aTACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑AT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCCTTCCa                                                                                                                                                                                              >  1:539034/1‑238 (MQ=255)

                                                         tgtctttgcacaCCTTGCCAGATGGTTTCGGGGA‑TGAACGGATTGAGCGGTTGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACGGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTcgcg                                                                                                                                                                                    <  2:117032/231‑1 (MQ=255)

                                                           tcctttcacccggcgctggtgtgTTTCGGGGA‑TGCACGGAATGATGTGATTGGCTTAGCGCAGATGCTCTTCCGGTACAGTCCGCGGCGTAGGGCGAGTTCCTCGCAGTTCTGCTTGGGTGCCACCGTTAATGAACGGCTTGGTCAGCACGGGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATTCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTcgcg                                                                                                                                                                                    <  2:87312/215‑1 (MQ=255)

                                                           tACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGatat                                                                                                                                                                                                                <  1:4922/209‑1 (MQ=255)

                                                            tctttccccccctcccggttGGTTTCGGTGA‑TGTACGGAATGCTTGGATGCGCGGGGCGCCGCAGGCCTTCCAGGACAGTCCCCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTcgcg                                                                                                                                                                                    <  2:682314/217‑1 (MQ=255)

                                                            aCTTTCGCCCGGTGCCCGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCgg                                                                                                                                                                                   <  1:501945/237‑1 (MQ=255)

                                                            aCTTTCCCACCCTGCCGGGTGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCGCCAGCGTATGGCGAGTTCCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCGCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTa                                                                                                                                                                                 <  2:1157964/239‑1 (MQ=255)

                                                              tttCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACg                                                                                                                                                                               <  2:73204/239‑1 (MQ=255)

                                                                   cgcgcgGCCAGGTGGTTGCGGTGA‑TGAACGGAATGATCGGATGGGCGGGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTg                                                                                                                                                                          <  2:1146899/237‑1 (MQ=255)

                                                                         gccggttGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGGGGGGCGCAGCAGTCCTTCCAGTACAGTCACCAGCGTAAGGCGAGTCCCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTc                                                                                                                                                                    <  2:673848/233‑1 (MQ=255)

                                                                         gCCGGATGTTTTCGGTGG‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATCCCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTc                                                                                                                                                                    <  2:382603/239‑1 (MQ=255)

                                                                         gCCAGATGGTTTCGGTGT‑GGAACGGAATGATCGGGTGCGCGAGGCGCCGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGGCCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTc                                                                                                                                                                    <  2:573483/239‑1 (MQ=255)

                                                                                 gTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTCCCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGaa                                                                                                                                                            <  2:967324/239‑1 (MQ=255)

                                                                                  tttCGGTGAGTGAGC‑GAATGGTGGGTTGGGCGAGGGCCGGCAGACCTGACAGGACAGTCACCAGCGTATGGCGAGTACCGCGCAGGTCTGCTGCGGTGCCACCGTTCATTACCGGCGTGGTCAGCGCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCTGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGCTTGaa                                                                                                                                                            <  2:542832/238‑1 (MQ=255)

                                                                                  tttCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGaa                                                                                                                                                            <  1:1028399/238‑1 (MQ=255)

                                                                                    tCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCCGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCCCGGGACGCTTTGATGGTCTGGTTGaaat                                                                                                                                                          >  2:226610/1‑236 (MQ=255)

                                                                                             gaACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAg                                                                                                                                                                                                     <  2:1113681/187‑1 (MQ=255)

                                                                                             gaACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAg                                                                                                                                                                                                     >  1:1113681/1‑187 (MQ=255)

                                                                                                             tGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCCCCGAACCTGTTCCAGGGGAA‑CT‑C‑ATACCGAATGCCTGCGGCGATATTCAAGGGGAAGCTGTCCAGCGCTTCGCGGTACCCTTTTGTGGTTTGGGTTAAACCCCCCCGAAACCACGGGgccgccgg                                                                                                                                 >  2:270473/1‑237 (MQ=255)

                                                                                                             tGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTCCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGGCTGGTTGAAATCCGCCCGAATCCAACGGTCCGCCa                                                                                                                                  >  1:735917/1‑238 (MQ=255)

                                                                                                                 cggggCGCCGGAGACCTTCCAGTACAGTCCCCGGCGTATGGGGAGTCCCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGTTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGa                                                                                                                              <  2:539034/235‑1 (MQ=255)

                                                                                                                 cgAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGcc                                                                                                                                                                                                                           >  1:1341741/1‑145 (MQ=255)

                                                                                                                 cgAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGcc                                                                                                                                                                                                                           <  2:1341741/145‑1 (MQ=255)

                                                                                                                         agcagACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAA‑AT‑C‑ATACAGGATGCCTGCGGCGATAACGAAGCGGAAGCGGTCCAGCGCTTCGCGGGACGCTTTGATGGTCCGGTTGAAACCCGCCAGAAACCAGCGGTCCGCCAGCGAAAGCGGCAt                                                                                                                     >  2:1162378/1‑239 (MQ=255)

                                                                                                                                          aGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCTCGGTACGCTTTTATTGGCTGGTTGAACTCCGCCAGAATCCATCGGTGCGCCATGGCCAGCGTCCTTTCGCCGCCGTTGAAg                                                                                                     >  1:1376921/1‑238 (MQ=255)

                                                                                                                                            tCACCAGCGTATGGCGAGTACCGCGTAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAAAATCCACCGGGCCGCCCGCGACAGCGCCCTTTTGCCCCCCCTGAAAcc                                                                                                   >  2:1247989/1‑238 (MQ=255)

                                                                                                                                            tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGCACTGGTTCCAGGGGAA‑CT‑C‑ATACAGAATGCCTGCGGCGCTATCGAAGCGGGAGCTGTCCAACGCTTCGCGGTACGCCTTGGTGGGCTGGTTGAACTCCGCCCGAATCCAGCGGTCCGCCAGCGGCAGCGGCAttttacccccctttaaacc                                                                                                   >  2:244589/1‑222 (MQ=255)

                                                                                                                                            tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGTAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGcc                                                                                                   >  1:710256/1‑238 (MQ=255)

                                                                                                                                            tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGGGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACACCGTCATTTCGCCGCCGTTGAAGcc                                                                                                   >  1:968188/1‑238 (MQ=255)

                                                                                                                                            tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGGTGAACTCCGCCCGAATCCAGCGGTCCGCCAGCGACAGCTTCATTTCGCCCCCGTTGAAGCCg                                                                                                  >  1:1192484/1‑239 (MQ=255)

                                                                                                                                            tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCCGCGATATCTAAGCGGAAGCTGTACCGCGCTTCGCGGTACGCTTTGATTGTCTGGTTGAACTCCGCCAGACTCCAGCGGTCCGCCCGCCACAACGTCATTTCCCCCCCCGTGAAGcc                                                                                                   >  1:570679/1‑238 (MQ=255)

                                                                                                                                               ccAGCGTATGGCGAGTACCGGCCAGTTCTGTTGCGGTGCCACCGTTCATTCCCGGCTTGGTCAGCTCGAGATCCCAGTCACAGAACGGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGGGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCaa                                                                                               <  1:244589/239‑1 (MQ=255)

                                                                                                                                                 aGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAAt                                                                                              >  1:989409/1‑238 (MQ=255)

                                                                                                                                                    gTATGGCGAGTACCGGGCATTTCTGCTTCGGTCCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATc                                                                                             <  1:503584/236‑1 (MQ=255)

                                                                                                                                                    gTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATCCCAGTCACAGAACTGGTCCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCt                                                                                           <  1:386558/238‑1 (MQ=255)

                                                                                                                                                     tATGGCGAGTACCGGGTAGTTCGGCTTCGGTCCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCAAAGAACTGGTTCCAGGTAAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTg                                                                                          <  1:1247989/238‑1 (MQ=255)

                                                                                                                                                     tATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGGACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCCGCGGTCCGCCAGCGACAGCGGCATTTCGCCGCCGTTGAAGCCCCAATCCTg                                                                                          >  1:995574/1‑238 (MQ=255)

                                                                                                                                                      aTGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGGTCCAGGGGAA‑CT‑C‑ATACAAAATGCCTGCGGCGATATCGAAGCGGAAGCTTTCCAGCGCTTCGCGGGACGCCTTGATGGGCTGGGTGAACTCCCCCAGAACCCAGCGGTCAGCCCGCGACGGCCGCATTTTGCCGCCCGTGAAACAGCaacaccga                                                                                         >  2:1263042/1‑232 (MQ=255)

                                                                                                                                                         gcgggtcccggccAGTTCCGCTTCGGGCCCACCGTTCATTCCCGGCTTGGTCAGCTCGAGATCCCAGTCAAAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCtt                                                                                     <  1:540228/227‑1 (MQ=255)

                                                                                                                                                          cggttccccggtttttttccTTGGGTGCCCCCTTTCTTTCCGCGCTGGGTCAGCGCGAAATCCCCGTCACAGAACGGGTTCCGGGTGAA‑CT‑C‑AAACAGAAGGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTCCGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCtt                                                                                     <  2:246831/219‑1 (MQ=255)

                                                                                                                                                            aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGGGATCCCAGTCCCAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTTCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGCCTGGTTGAACTCCGCCAAAATTCGACGGGCCGCCAGCGACAGCGTCATTTCCCCGCCGTTGAAGCCGCAATCCCAACCTTCtg                                                                                  >  1:558393/1‑239 (MQ=255)

                                                                                                                                                            aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCACCGGTCGGCCAGCGACAGCGTCATTTCGCCGCCGGTGAAGCCGCAACCCCTAcctcctc                                                                                  >  1:1356905/1‑238 (MQ=255)

                                                                                                                                                              ttccgccacgtcttcgttCGGTGCCCCTGTTCATTCCGGGGTGGGTCAGCTGGAGAAACCAGTCA‑AAAACCGGGTTCAGGGGAACCC‑C‑ATACAGAATGTCTGCGGTGATATCCAAGCGGAAGCGTTCAAGCGCTTCGGGGTACGCTTTGATGGCCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTCCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                <  2:1135277/223‑1 (MQ=255)

                                                                                                                                                              tACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCCGCGGCGATAACGAAGCGGACACTGTACAGCCCCTCCCGGGAGGCTTTGGTGGGCTGGTTGAAACCCCCCCGAAACCACCGGGCCGCCAGCGAACACGGCCTTTTGCCCCCGTTGAAGCCCCacacccgccctcctggg                                                                                >  2:386558/1‑223 (MQ=255)

                                                                                                                                                              ccccgcctgttttccgttgggTCCCACTGTCCTTTACCGGGTTGGTCAGCTCGAGATACCAGTCACAGAACTTTTCCAAGGTGAA‑CT‑C‑ATCCAGAAGGCCGGCGGCGATTTCGAAGCGGAAGCTGCCCAGCGCTTCGCGGTACGCTTTGATGGTTTGGTTGACCCCCGCCAGAACCCAGCGTTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                <  2:752807/220‑1 (MQ=255)

                                                                                                                                                               cccctgcgtttCTGCGTCGGTGCCACCCTTCCTTACCGGCGTGGTCCGCTCGGGATACCGGTCAGAGAACGGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCGGCGGCGATATCGAAGCGGAAGCTTTCCAGCGCTTCGCGGTACGCTTTGAGGGTCTGGTTGAACCCCGCAAGAATCCAGCGGTCCGCCAGGGAGAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                <  2:1198479/229‑1 (MQ=255)

                                                                                                                                                               cccccgcgtttcttttccGGTGCCCCCGTTCATTACCGGCTTGGTCAGCTCGAGATCCCAGTCACAGAATTGGTTCCAGGTGAA‑CT‑A‑AAACAGAATGCCTGCGGGGATAGCGAAGCGGAAGCTGTCCAGCGTTGCGCGGTCCGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                <  2:1299425/221‑1 (MQ=255)

                                                                                                                                                               cccGCGCATTTCTGCTTGGGTGCCCCCGTTCATTCCCGGCTTGGTCAGCGCGAGACCCCAGTCACAGAAGTGGTCCCAGGTGAA‑CC‑A‑ATGCAGAATGCCGGCGGCGATATCGAGGCGGAAGCTGGCCAGCGCTTCGCGGTCCGTTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                <  2:856015/237‑1 (MQ=255)

                                                                                                                                                                   cgcAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCCGGGGAA‑CT‑A‑ATAAAGAATTCCCGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCCTCGCGGTACGCTTTGGTGGTCTGGGTGAACTCCGCCAGAATCCAGCGGTCCGCCAGGCACCGCGGCAGTTGCCCCCCCGTGAAGGCGCaaaaccgaacgtctgtttgaag                                                                           >  2:860324/1‑219 (MQ=255)

                                                                                                                                                                   cgcAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtca                                                                            >  1:246831/1‑238 (MQ=255)

                                                                                                                                                                   cgcAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAA‑CT‑C‑ATACAGAATGCCTGCCGCGATATAGAAGCGGGAGCTGTCCCGCGCTTCCCGGTACGCTTTGGTGGGTCGGGTGAACTCCGCCGGAATCCAGCGGGACCCCAGGGCAACCGTCAATTACCCGCCGTTTAGACCGCaaaacggcccctcgtgggtctc                                                                           >  2:165217/1‑219 (MQ=255)

                                                                                                                                                                        ttCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTAAGCTCGAGAAACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAAATCCGCCAGAATCCAGGGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGGTGAAGCCGCAATCCTGACCTTCGTTGTTCATCAGcc                                                                      >  1:1323251/1‑238 (MQ=255)

                                                                                                                                                                             cTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAg                                                                 >  1:579472/1‑239 (MQ=255)

                                                                                                                                                                                  gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCCGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCCCCCCCGTTGAAGCCCCAATCCTTACCTTCTTGGTTCATCACCCCAAAACg                                                               >  1:288523/1‑236 (MQ=255)

                                                                                                                                                                                  gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCCGCACAAAggc                                                               >  1:1166866/1‑234 (MQ=255)

                                                                                                                                                                                  gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCCCAATCCTGACCTTTTGGGTTCACCACACCAAAGCg                                                               >  1:367618/1‑236 (MQ=255)

                                                                                                                                                                                  gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTTGCGGTACGCTTTGATGGTTTGGTTGAAATCCGCCAGAATCCACCGGGCCGCCAGCGGCAGCGTCATTTCGCCGCCGTTGAAGCCGAAATCCCGCCCTTCttgggtcacaagaccaaaaggggg                                                            >  2:688473/1‑216 (MQ=255)

                                                                                                                                                                                  gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAACGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGGGTTCATCAGCACAAAgag                                                               >  1:293628/1‑234 (MQ=255)

                                                                                                                                                                                       aCCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCCCAAAGCGGCTGGCGt                                                       >  1:360314/1‑239 (MQ=255)

                                                                                                                                                                                             cATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAACGGAAGCCGTCCAGCGCTTCGCCGTACGCTTTGATGGTCTGGTTGTACTCCGCCAGAATCCAACGGCCCGCCACCGAAGACGTCGTTTCGCCGCCGTTTAAGCCGCAATCCCGACCTGCTGCGGTCATCTGCACAAAACgccgggactccccaa                                                 >  2:1051036/1‑225 (MQ=255)

                                                                                                                                                                                                     gCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcat                                                                           >  1:1109258/1‑205 (MQ=255)

                                                                                                                                                                                                     gCTTGGGCAGCTCGAGATACCAGTCACAGAACTGGTTCCACGGGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTTAACTCCCCCAGAATTCAGCGGCCACCCAGAGACACCGTTATTTCTCCGCCTTTCAAGCCCCCATCCTGACCCTCCGTGTTCACCAACCCAAAGGGGCGGGCGGTCcaccaccttctc                                         >  2:987998/1‑230 (MQ=255)

                                                                                                                                                                                                           tctgccgcgatccccAGTCACGGAGCGGGTTCCGGGGAAC‑CT‑A‑AACCAGACGGCCTGCGGCATTATCAAGGCGAATGCTGGCCAGCGCTGCGCGGTACGCTTGGGTGGTCTGGTTGACCTCCGCCAGAACCCAGCGGTCCGCCAGCGACAGCTTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAg                                   <  2:710256/226‑1 (MQ=255)

                                                                                                                                                                                                               cTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCCGCCCAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTAc                               >  1:169/1‑239 (MQ=255)

                                                                                                                                                                                                               cTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGAGTTCCACAGCTTGTTACAGAAGTTAc                               >  1:840207/1‑239 (MQ=255)

                                                                                                                                                                                                                  gagttcccgtgccaaGAACTGGTTCCAGGGGAA‑CG‑T‑ATCCAGGATGCCGGCGGCGATCTCGAAGCGGAAGCTGCCCGGCGTTTCGCGGTACGCTTTGATGGTCTGGTTGACCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGt                            <  2:968188/225‑1 (MQ=255)

                                                                                                                                                                                                                     atcccAGTCCCAGAACTGGTGCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAAc                         <  1:1237005/236‑1 (MQ=255)

                                                                                                                                                                                                                         cAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcat                                                                           >  1:513357/1‑185 (MQ=255)

                                                                                                                                                                                                                         cAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCca                                                   >  1:1178578/1‑209 (MQ=255)

                                                                                                                                                                                                                         cAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTACAGCCGCAATCCTTACCTTCTGTGTTCATCAGCACAAAACGCCTGGGGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTc                     >  2:936761/1‑239 (MQ=255)

                                                                                                                                                                                                                             ccaaaaaCTGGTTCCAGGTTAA‑CT‑C‑ATACAGAATGCCTGCGGCGATTGCGAAGCGGAAGCTGTCCAGCGCTTCGCGTTACGTTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAg                  <  1:141720/233‑1 (MQ=255)

                                                                                                                                                                                                                             cacaGAACTGGTTCCAGGTGAC‑CT‑C‑ATACAGAATCCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                 <  1:165217/239‑1 (MQ=255)

                                                                                                                                                                                                                             cacaGAACGGGTCCCGGGTGAA‑CT‑C‑ATAAAGAAGGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTCCGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                 <  1:226610/239‑1 (MQ=255)

                                                                                                                                                                                                                              ctaagaCTGGGTCCAGGGGGA‑CC‑CAATACAGAATTCCGGGGGCGATTTGGAGGGAGAACCTGTCCAGCCGTTCGCGTTCCGCTTTGATGGTCGGGTTAACCTCCGCCAGAATCCAGGGATCCGCCGGGGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                 <  2:617085/234‑1 (MQ=255)

                                                                                                                                                                                                                              acaGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGCCTGGCGTTCCCCACCTTGTTACAAAAGTTACGGAAACACTCCAGAc                >  2:714525/1‑239 (MQ=255)

                                                                                                                                                                                                                              acaGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAACAGGCGGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                 >  1:665007/1‑238 (MQ=255)

                                                                                                                                                                                                                               caGAACTGGTTCCAGGTGAC‑CT‑C‑ATCCAGAATGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGTTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACg               <  1:1306612/239‑1 (MQ=255)

                                                                                                                                                                                                                               aagacgtGGTTCC‑GGGGAA‑CTCC‑ATACAGAATGCCTGGGGCGATAGCGAAGCGGACGCTGCCCAGCGCTTCGCGGTACGCTTTGAGGGTCTGGTTGAACCCCGCCAGAAGCCAGCGGTCCGCCAGCGACAGCTTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACg               <  2:665007/233‑1 (MQ=255)

                                                                                                                                                                                                                                       gTTCCAGGTGTA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGCATCCAGCGTTCCGCCCGCGACAGCTGCATTTCGCCGCCGTTGAAGCCGCCATCCTTACCATCTGTGTCCATCAGCACAAAGCGGCGGCCGTCCCACAGCTTGTTACGGAAATTACGGTAACCTTCCCACCGCTTCata        >  2:1111546/1‑238 (MQ=255)

                                                                                                                                                                                                                                       gTTCCAGGTGAC‑CT‑C‑ATACAGAATGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCa          <  1:1029990/236‑1 (MQ=255)

                                                                                                                                                                                                                                       gTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCCCAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGCCGCTTCcta        >  1:617085/1‑238 (MQ=255)

                                                                                                                                                                                                                                        ttCCAGGTGAA‑CC‑A‑AAACAGAATGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCatat       <  1:213869/238‑1 (MQ=255)

                                                                                                                                                                                                                                            aGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCGGAATCCACCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAACCCGCAATCCTCGCCTTCTGTGTCCATCAGCACAACGCGGCTGCCGTTCCCCACATTTTTCTAAAAGTTAACGCAACCTTCCaaaacccccataacacag  >  2:662140/1‑222 (MQ=255)

                                                                                                                                                                                                                                            aGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGCCCTTCTGGGTTCATTAGCACAAAACGGCGGGCGGTCCACACCTTATTCCAGAAGTTAGGGGAACCTTCCAAACGCTTCAAATCCCAg  >  2:235825/1‑239 (MQ=255)

                                                                                                                                                                                                                                            aGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCCCACAGCGGCTGGCGTTCCACAGCTTGTTCCAGAAGTTACGGTAACCTTCCAGCCGCTTCAATTCCCAg  >  1:612314/1‑239 (MQ=255)

GATGCATCGTACTGCGGGAACGGCTGCAGCATGATGGTGTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGA‑TGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAA‑CT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAG  >  NC_000913/4481479‑4481952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`