BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,481,800	T→C	14.5%	E680G (GAG→GGG)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,481,800	0	T	C	14.5%	321.5 / 7.6	133	E680G (GAG→GGG)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (79/29); new base C (3/21); total (82/51)
Fisher's exact test for biased strand distribution p-value = 4.61e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAA  >  NC_000913/4481587‑4482035

tGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTCCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGGCTGGTTGAAATCCGCCCGAATCCAACGGTCCGCCa                                                                                                                                                                                                                     >  1:735917/1‑238 (MQ=255)

    cggggCGCCGGAGACCTTCCAGTACAGTCCCCGGCGTATGGGGAGTCCCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGTTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGa                                                                                                                                                                                                                 <  2:539034/235‑1 (MQ=255)

                             aGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCTCGGTACGCTTT‑TATTGGCTGGTTGAACTCCGCCAGAATCCATCGGTGCGCCATGGCCAGCGTCCTTTCGCCGCCGTTGAAg                                                                                                                                                                                        >  1:1376921/1‑238 (MQ=255)

                               tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGCACTGGTTCCAGGGGAACT‑C‑ATACAGAATGCCTGCGGCGCTATCGAAGCGGGAGCTGTCCAACGCTTCGCGGTACGCCTT‑GGTGGGCTGGTTGAACTCCGCCCGAATCCAGCGGTCCGCCAGCGGCAGCGGCAttttacccccctttaaacc                                                                                                                                                                                      >  2:244589/1‑222 (MQ=255)

                               tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGTAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGcc                                                                                                                                                                                      >  1:710256/1‑238 (MQ=255)

                               tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGGTGAACTCCGCCCGAATCCAGCGGTCCGCCAGCGACAGCTTCATTTCGCCCCCGTTGAAGCCg                                                                                                                                                                                     >  1:1192484/1‑239 (MQ=255)

                               tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCCGCGATATCTAAGCGGAAGCTGTACCGCGCTTCGCGGTACGCTTT‑GATTGTCTGGTTGAACTCCGCCAGACTCCAGCGGTCCGCCCGCCACAACGTCATTTCCCCCCCCGTGAAGcc                                                                                                                                                                                      >  1:570679/1‑238 (MQ=255)

                                  ccAGCGTATGGCGAGTACCGGCCAGTTCTGTTGCGGTGCCACCGTTCATTCCCGGCTTGGTCAGCTCGAGATCCCAGTCACAGAACGGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGGGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCaa                                                                                                                                                                                  <  1:244589/239‑1 (MQ=255)

                                    aGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAAt                                                                                                                                                                                 >  1:989409/1‑238 (MQ=255)

                                       gTATGGCGAGTACCGGGCATTTCTGCTTCGGTCCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATc                                                                                                                                                                                <  1:503584/236‑1 (MQ=255)

                                       gTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATCCCAGTCACAGAACTGGTCCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCt                                                                                                                                                                              <  1:386558/238‑1 (MQ=255)

                                        tATGGCGAGTACCGGGTAGTTCGGCTTCGGTCCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCAAAGAACTGGTTCCAGGTAAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTg                                                                                                                                                                             <  1:1247989/238‑1 (MQ=255)

                                        tATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGGACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCCGCGGTCCGCCAGCGACAGCGGCATTTCGCCGCCGTTGAAGCCCCAATCCTg                                                                                                                                                                             >  1:995574/1‑238 (MQ=255)

                                         aTGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGGTCCAGGGGAACT‑C‑ATACAAAATGCCTGCGGCGATATCGAAGCGGAAGCTTTCCAGCGCTTCGCGGGACGCCTT‑GATGGGCTGGGTGAACTCCCCCAGAACCCAGCGGTCAGCCCGCGACGGCCGCATTTTGCCGCCCGTGAAACAGCaacaccga                                                                                                                                                                            >  2:1263042/1‑232 (MQ=255)

                                            gcgggtcccggccAGTTCCGCTTCGGGCCCACCGTTCATTCCCGGCTTGGTCAGCTCGAGATCCCAGTCAAAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCtt                                                                                                                                                                        <  1:540228/227‑1 (MQ=255)

                                             cggttccccggtttttttccTTGGGTGCCCCCTTTCTTTCCGCGCTGGGTCAGCGCGAAATCCCCGTCACAGAACGGGTTCCGGGTGAACT‑C‑AAACAGAAGGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTCCGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCtt                                                                                                                                                                        <  2:246831/219‑1 (MQ=255)

                                               aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGGGATCCCAGTCCCAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTTCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGCCTGGTTGAACTCCGCCAAAATTCGACGGGCCGCCAGCGACAGCGTCATTTCCCCGCCGTTGAAGCCGCAATCCCAACCTTCtg                                                                                                                                                                     >  1:558393/1‑239 (MQ=255)

                                               aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCACCGGTCGGCCAGCGACAGCGTCATTTCGCCGCCGGTGAAGCCGCAACCCCTAcctcctc                                                                                                                                                                     >  1:1356905/1‑238 (MQ=255)

                                                 tACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCCGCGGCGATAACGAAGCGGACACTGTACAGCCCCTCCCGGGAGGCTTT‑GGTGGGCTGGTTGAAACCCCCCCGAAACCACCGGGCCGCCAGCGAACACGGCCTTTTGCCCCCGTTGAAGCCCCacacccgccctcctggg                                                                                                                                                                   >  2:386558/1‑223 (MQ=255)

                                                 ccccgcctgttttccgttgggTCCCACTGTCCTTTACCGGGTTGGTCAGCTCGAGATACCAGTCACAGAACTTTTCCAAGGTGAACT‑C‑ATCCAGAAGGCCGGCGGCGATTTCGAAGCGGAAGCTGCCCAGCGCTTCGCGGTACGCTTT‑GATGGTTTGGTTGACCCCCGCCAGAACCCAGCGTTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                                                                                                   <  2:752807/220‑1 (MQ=255)

                                                  cccctgcgtttCTGCGTCGGTGCCACCCTTCCTTACCGGCGTGGTCCGCTCGGGATACCGGTCAGAGAACGGGTTCCAGGTGAACT‑C‑ATACAGAATGCCGGCGGCGATATCGAAGCGGAAGCTTTCCAGCGCTTCGCGGTACGCTTT‑GAGGGTCTGGTTGAACCCCGCAAGAATCCAGCGGTCCGCCAGGGAGAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                                                                                                   <  2:1198479/229‑1 (MQ=255)

                                                  cccGCGCATTTCTGCTTGGGTGCCCCCGTTCATTCCCGGCTTGGTCAGCGCGAGACCCCAGTCACAGAAGTGGTCCCAGGTGAACC‑A‑ATGCAGAATGCCGGCGGCGATATCGAGGCGGAAGCTGGCCAGCGCTTCGCGGTCCGTTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCtgtg                                                                                                                                                                   <  2:856015/237‑1 (MQ=255)

                                                      cgcAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCCGGGGAACT‑A‑ATAAAGAATTCCCGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCCTCGCGGTACGCTTT‑GGTGGTCTGGGTGAACTCCGCCAGAATCCAGCGGTCCGCCAGGCACCGCGGCAGTTGCCCCCCCGTGAAGGCGCaaaaccgaacgtctgtttgaag                                                                                                                                                              >  2:860324/1‑219 (MQ=255)

                                                      cgcAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtca                                                                                                                                                               >  1:246831/1‑238 (MQ=255)

                                                      cgcAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAACT‑C‑ATACAGAATGCCTGCCGCGATATAGAAGCGGGAGCTGTCCCGCGCTTCCCGGTACGCTTT‑GGTGGGTCGGGTGAACTCCGCCGGAATCCAGCGGGACCCCAGGGCAACCGTCAATTACCCGCCGTTTAGACCGCaaaacggcccctcgtgggtctc                                                                                                                                                              >  2:165217/1‑219 (MQ=255)

                                                           ttCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTAAGCTCGAGAAACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAAATCCGCCAGAATCCAGGGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGGTGAAGCCGCAATCCTGACCTTCGTTGTTCATCAGcc                                                                                                                                                         >  1:1323251/1‑238 (MQ=255)

                                                                     gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCCGCACAAAggc                                                                                                                                                  >  1:1166866/1‑234 (MQ=255)

                                                                     gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCCCAATCCTGACCTTTTGGGTTCACCACACCAAAGCg                                                                                                                                                  >  1:367618/1‑236 (MQ=255)

                                                                     gTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGGGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAACGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGGGTTCATCAGCACAAAgag                                                                                                                                                  >  1:293628/1‑234 (MQ=255)

                                                                          aCCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCCCAAAGCGGCTGGCGt                                                                                                                                          >  1:360314/1‑239 (MQ=255)

                                                                                cATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAACGGAAGCCGTCCAGCGCTTCGCCGTACGCTTT‑GATGGTCTGGTTGTACTCCGCCAGAATCCAACGGCCCGCCACCGAAGACGTCGTTTCGCCGCCGTTTAAGCCGCAATCCCGACCTGCTGCGGTCATCTGCACAAAACgccgggactccccaa                                                                                                                                    >  2:1051036/1‑225 (MQ=255)

                                                                                        gCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTtcat                                                                                                                                                              >  1:1109258/1‑205 (MQ=255)

                                                                                        gCTTGGGCAGCTCGAGATACCAGTCACAGAACTGGTTCCACGGGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTTAACTCCCCCAGAATTCAGCGGCCACCCAGAGACACCGTTATTTCTCCGCCTTTCAAGCCCCCATCCTGACCCTCCGTGTTCACCAACCCAAAGGGGCGGGCGGTCcaccaccttctc                                                                                                                            >  2:987998/1‑230 (MQ=255)

                                                                                              tctgccgcgatccccAGTCACGGAGCGGGTTCCGGGGAACCT‑A‑AACCAGACGGCCTGCGGCATTATCAAGGCGAATGCTGGCCAGCGCTGCGCGGTACGCTTG‑GGTGGTCTGGTTGACCTCCGCCAGAACCCAGCGGTCCGCCAGCGACAGCTTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAg                                                                                                                      <  2:710256/226‑1 (MQ=255)

                                                                                                  cTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCCGCCCAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTAc                                                                                                                  >  1:169/1‑239 (MQ=255)

                                                                                                  cTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGAGTTCCACAGCTTGTTACAGAAGTTAc                                                                                                                  >  1:840207/1‑239 (MQ=255)

                                                                                                     gagttcccgtgccaaGAACTGGTTCCAGGGGAACG‑T‑ATCCAGGATGCCGGCGGCGATCTCGAAGCGGAAGCTGCCCGGCGTTTCGCGGTACGCTTT‑GATGGTCTGGTTGACCTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGt                                                                                                               <  2:968188/225‑1 (MQ=255)

                                                                                                        atcccAGTCCCAGAACTGGTGCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAAc                                                                                                            <  1:1237005/236‑1 (MQ=255)

                                                                                                            cAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCca                                                                                                                                      >  1:1178578/1‑209 (MQ=255)

                                                                                                            cAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTACAGCCGCAATCCTTACCTTCTGTGTTCATCAGCACAAAACGCCTGGGGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTc                                                                                                        >  2:936761/1‑239 (MQ=255)

                                                                                                                cacaGAACTGGTTCCAGGTGACCT‑C‑ATACAGAATCCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                                                                                                    <  1:165217/239‑1 (MQ=255)

                                                                                                                 ctaagaCTGGGTCCAGGGGGACC‑CAATACAGAATTCCGGGGGCGATTTGGAGGGAGAACCTGTCCAGCCGTTCGCGTTCCGCTTT‑GATGGTCGGGTTAACCTCCGCCAGAATCCAGGGATCCGCCGGGGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                                                                                                    <  2:617085/234‑1 (MQ=255)

                                                                                                                 acaGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGCCTGGCGTTCCCCACCTTGTTACAAAAGTTACGGAAACACTCCAGAc                                                                                                   >  2:714525/1‑239 (MQ=255)

                                                                                                                  caGAACTGGTTCCAGGTGACCT‑C‑ATCCAGAATGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGTTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACg                                                                                                  <  1:1306612/239‑1 (MQ=255)

                                                                                                                  aagacgtGGTTCC‑GGGGAACTCC‑ATACAGAATGCCTGGGGCGATAGCGAAGCGGACGCTGCCCAGCGCTTCGCGGTACGCTTT‑GAGGGTCTGGTTGAACCCCGCCAGAAGCCAGCGGTCCGCCAGCGACAGCTTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACg                                                                                                  <  2:665007/233‑1 (MQ=255)

                                                                                                                          gTTCCAGGTGTACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGCATCCAGCGTTCCGCCCGCGACAGCTGCATTTCGCCGCCGTTGAAGCCGCCATCCTTACCATCTGTGTCCATCAGCACAAAGCGGCGGCCGTCCCACAGCTTGTTACGGAAATTACGGTAACCTTCCCACCGCTTCata                                                                                           >  2:1111546/1‑238 (MQ=255)

                                                                                                                          gTTCCAGGTGACCT‑C‑ATACAGAATGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCa                                                                                             <  1:1029990/236‑1 (MQ=255)

                                                                                                                           ttCCAGGTGAACC‑A‑AAACAGAATGCCGGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCatat                                                                                          <  1:213869/238‑1 (MQ=255)

                                                                                                                               aGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCGGAATCCACCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAACCCGCAATCCTCGCCTTCTGTGTCCATCAGCACAACGCGGCTGCCGTTCCCCACATTTTTCTAAAAGTTAACGCAACCTTCCaaaacccccataacacag                                                                                     >  2:662140/1‑222 (MQ=255)

                                                                                                                               aGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCCCACAGCGGCTGGCGTTCCACAGCTTGTTCCAGAAGTTACGGTAACCTTCCAGCCGCTTCAATTCCCAg                                                                                     >  1:612314/1‑239 (MQ=255)

                                                                                                                                    aaCT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGAt                                                                                >  1:1018912/1‑239 (MQ=255)

                                                                                                                                    aaCT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCGGACCTTCTGTGTTCATCCGCACAAAGCGGCTGGCAGTCCACCGATTGTTACCGAACTTACGGGAACCTTCCAACCGCCTCCTAATCCAGtgtta                                                                                >  2:1291558/1‑235 (MQ=255)

                                                                                                                                    aaCT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCCCACTCCTGACCTTCTGTGTTCCTCAGACCAAAGCGGCGGGCGTTCCACAGCTTGTTACAGAATTTCCTGTAACCTTCCCGACGCTACATAGCCCAGtt                                                                                   >  2:1029990/1‑236 (MQ=255)

                                                                                                                                                  aTGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAACCGGCTGGCGTTCCACCGCTGGTTCCCAAAGTTACGGTAACCTTCCAGACGCTTCCAATCCCCGTTGATGACACCACCgga                                                                    >  2:519384/1‑238 (MQ=255)

                                                                                                                                                   tGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCtt                                                                                                                                                                        >  2:688347/1‑138 (MQ=255)

                                                                                                                                                   tGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCtt                                                                                                                                                                        <  1:688347/138‑1 (MQ=255)

                                                                                                                                                   tGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGATTCATATCCCCGTTGATGTCTCGACCGGTa                                                                   >  1:105530/1‑239 (MQ=255)

                                                                                                                                                   tGCCTGCGGCGATATCGAACCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCCGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCCCAAAGCGGCTGGCGTTCCAACACTTGTTACAGAAGTTACGGTAACCTTCCCGACGCTTCCTATACCAGtttctgacccgcccggaa                                                                   >  2:1142163/1‑223 (MQ=255)

                                                                                                                                                      cTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGCCCGGTaca                                                                 >  1:1225873/1‑236 (MQ=255)

                                                                                                                                                       tGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCCGTTGATGTCACAACCGGTAAAc                                                                >  2:1147481/1‑238 (MQ=255)

                                                                                                                                                           gcgATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTCCAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTAATTTACCGACCGGTAGAccgccg                                                           >  2:1222066/1‑234 (MQ=255)

                                                                                                                                                            gtttttcaggggggAAGCTGTCCA‑CGGTTTGGGGTTCCCTTTGGGGGGTCGGGTGAACCCCCCCCACAACCCAGGCGTCCGCAAGCGACAGGGTCATTCCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAgc                                                          <  2:1323251/227‑1 (MQ=255)

                                                                                                                                                            gaaaatCGAACGGGAAGCTGTCCAGCCCTTCGCGGTTCGCTTG‑GAGGGTCTGGTTGAACCCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAg                                                           <  1:270473/234‑1 (MQ=255)

                                                                                                                                                                atCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GAGGGTCTGGTTGACCCCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAgccgct                                                      <  1:502749/239‑5 (MQ=255)

                                                                                                                                                                   gAAGCGGAAGCTGACCGGCGCTTCGGGGTACGCTTT‑GATGTGCTGGTTGAACGCCGCCATAATCCAGCGGTCCGCCGGCGACAGTGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCa                                                   <  1:688473/239‑1 (MQ=255)

                                                                                                                                                                         gAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGt                                                                                                               <  1:336224/173‑1 (MQ=255)

                                                                                                                                                                         gAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGGGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGt                                                                                                               >  2:336224/1‑173 (MQ=255)

                                                                                                                                                                               gTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTCCAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACCCCAGCGCCGCCAGGGTGAagcgc                                        >  1:938437/1‑238 (MQ=255)

                                                                                                                                                                                tcccggtcTTCGCGGTGCGTTTT‑GTTGGTCTGTGTGAACTCCGCCAGAACCCAGCGGTCCGGCGGCGACGGCGTCATTTCGGCGCCGTTGAAGCCGGAATTCTGACCTTCTGTGTTCATCAGCACAAAGCGGCGGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAagcgcag                                      <  2:268877/232‑1 (MQ=255)

                                                                                                                                                                                gccgccgcTCCGGGGTCCGGTTT‑GTTGTTCTGTTTGACCCCCGCCGAAAGCCAGTGTTCCGCACCAGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGGCCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAagcgcag                                      <  2:1018912/234‑1 (MQ=255)

                                                                                                                                                                                cccggcgtTTCGGGGTACGTTTT‑GATGGTCGTGTTGACCCCCGCCAGAATCCCGCGGTCCGCCAGCGACGGGGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAagcgcag                                      <  2:288523/235‑1 (MQ=255)

                                                                                                                                                                                cccagcctttgcgggttcctttt‑GGTGGTTTGGTTGACCCCCCCAAAAATCCGGGGTTCCGCCAGGGCACGCGTCATTCCCCCGCCCTTGAAGCCGCAAACCTGACCTTCTGTTTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAagcgcag                                      <  2:655082/219‑1 (MQ=255)

                                                                                                                                                                                 ccacggcTTCGGGGTACGGTGT‑GATGGTGTGGTTGAACTCCGACAGGATCCAGCGGTCCGCAAGCGCCAGCGTCATTTCGCCGGCGTTGAAGCCGCAATCCTGACCTTCTGTGGTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAagcgcag                                      <  1:1111546/233‑1 (MQ=255)

                                                                                                                                                                                 ccAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCCACGCCGCCAGGGGGAagggcaag                                     >  2:168571/1‑237 (MQ=255)

                                                                                                                                                                                 ccAGCGCTGCGCGGTACGTTTT‑GATGGTCTGGTTGACCCCCGCCAGAAGCCAGCGGTCCGCCAGCGACAGCGTCATTCCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAagcgcag                                      <  1:657672/238‑1 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GTTGGTCTGGTTGAACTCCGCCAGACTCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTCGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATAGCCCCGTTGATGTCACGACCGGTAGACGCCCGCgccccccaggggcagacaatcgcgcgaga                             >  2:921862/1‑215 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCca                                                                                                                                      <  1:81777/133‑1 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCCCAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCCCGACCCGTAGACGCCAGCCCCGCCCGGGGGAaggcccgcgccccacg                             >  2:115372/1‑224 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                                                                                                    >  2:1096711/1‑167 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGa                                                                                                    <  1:1096711/167‑1 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGt                             >  1:395009/1‑238 (MQ=255)

                                                                                                                                                                                          cgcgGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCCCCAGGGGGAagcgcagccgggcagg                             >  2:1061056/1‑230 (MQ=255)

                                                                                                                                                                                           gggttttctttttgagtgttgtgTTGACCCCCGCCAAACCCCAGCGGTCCGCCAGCGAACGCTTCATTCCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGtc                            <  2:367618/217‑2 (MQ=255)

                                                                                                                                                                                                gcgtttt‑GGTGGGTTGTTTGACCCCCGCAAGAACCCCGCGGCCCCCAAGCGACAGGGCTATTCCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTg                       <  2:612314/234‑1 (MQ=255)

                                                                                                                                                                                                aCGTTTT‑GAGGGTTTTGTGGAACCCCGCCAGAACCCCGCGGTCCGCCAGCGACAGCTTAATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTg                       <  1:1051036/238‑1 (MQ=255)

                                                                                                                                                                                                aCGCTTT‑GAGGGTCTGGTTGAACCCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTg                       <  1:476275/238‑1 (MQ=255)

                                                                                                                                                                                                 ccccttgggtggtttggttGAACCCCGCCAGAATCCAGGGGTCCGCCGGCGACCGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTg                       <  1:1358999/229‑1 (MQ=255)

                                                                                                                                                                                                   gtttgggtGGTCTGGTTGACCTCCGCAAAAACCCGGCGGTCCGCCAGCGACAGCGTAATTTCCCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCgg                    <  1:971619/232‑1 (MQ=255)

                                                                                                                                                                                                      t‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCCGGGGGAAGCGCAGCCCGTCAGTACCGTGCGGcccc                >  1:608580/1‑236 (MQ=255)

                                                                                                                                                                                                          tGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAAt              >  1:634574/1‑238 (MQ=255)

                                                                                                                                                                                                            gTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGCCCGGTAGACACCAGCGCCGCCAGGGTGAAGCGCAGGGCCTCAGTACCGTGCGGCCCAAGAcc           >  1:1300122/1‑239 (MQ=255)

                                                                                                                                                                                                            gTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGGTCCCCAGCTTGTTACAGAAGTTACGGTAACGCTCCAGACGATTGATCTCCCCGTTGATGTCGCGCCCGGCATACGCCAAGGCCGCCAGGGCGCAGCGCCGCGCGTCAGGACCGTTGGGCCCaaccac           >  2:1182588/1‑235 (MQ=255)

                                                                                                                                                                                                              ttgtttGAACTCCGCAAGACTCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCg          <  1:860324/234‑1 (MQ=255)

                                                                                                                                                                                                              cTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAATTACGGTAACCTTCCAGACGCTTCATATCCCAGTTTATTTCCCGACCGGAAGCGGCACGCCCCGCCAGGGTGAAGCGACTGGGGTCAGAACCGTGCgcccaaaacaagt         >  2:1297166/1‑227 (MQ=255)

                                                                                                                                                                                                               tGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAACGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTCGACTCCAGCGCCGCCAGGGGGAAACCCGCCGCGGCAGGACCGGGGGGCCCAAAACCGt         >  2:277987/1‑238 (MQ=255)

                                                                                                                                                                                                               tGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGCCGCTTCCTATCCCAGTTGATGTCCCGACCGGTAGACCCCCCCGCCCCCCGGGTGAACCGCACCCCGTCactccccgtcggcccaaccccct         >  2:573840/1‑216 (MQ=255)

                                                                                                                                                                                                               tGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGt         >  1:616137/1‑238 (MQ=255)

                                                                                                                                                                                                               tGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGGAGACCCCAGCGCCCCCAGGGGGAAGCGCAGGGCGTCAGAACCGGGCGGCCCacaacgttt        >  2:448823/1‑231 (MQ=255)

                                                                                                                                                                                                                 gTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCACCGCGTCAGTCCCGAGCGGCTCAATACCGTTCg      >  1:656931/1‑239 (MQ=255)

                                                                                                                                                                                                                   tGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATa             >  1:923288/1‑230 (MQ=255)

TGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACT‑C‑ATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTT‑GATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAA  >  NC_000913/4481587‑4482035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`