Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,549,431 G→T 11.4% C208F (TGT→TTT)  fimH → minor component of type 1 fimbriae

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,4310GT11.4% 428.6 / 3.3 144C208F (TGT→TTT) fimHminor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base G (61/63);  new base T (18/2);  total (79/65)
Fisher's exact test for biased strand distribution p-value = 5.26e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

ACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTATGCACGTACCGGAGGGCAGGTGACTGC  >  NC_000913/4549199‑4549665
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            
aCGCCTTTGGGCTGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAAGTGCCGTGCTTATTTTGCGACCGACCAACAACTATAACAGCGATGATTTCCAGTGTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTGCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGaa                                                                                                                                                                                                                                        <  1:819370/239‑1 (MQ=255)
  gccgggtggCAGTGGGGGGGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGaaa                                                                                                                                                                                                                                       <  2:1049577/230‑1 (MQ=255)
    cTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTTTTCCCGGGATGTCACCGTTACTCTTCCGGGCTACCCCGGTTCAGTGCCCATTCCTCTTTCCGTTTATTGTGGGAAAAg                                                                                                                                                                                                                                     >  1:522265/1‑238 (MQ=255)
     tggtaGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAGGcc                                                                                                                                                                                                                                   <  1:861199/235‑1 (MQ=255)
      gtgAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTCCTCTGCCCGACTAACCCGGTTCAGTGCCAATTCCCCTTACCGTTTATTTTTCGGAAACCCa                                                                                                                                                                                                                                  >  1:670733/1‑239 (MQ=255)
      gtgAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGGTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCa                                                                                                                                                                                                                                  >  1:492806/1‑239 (MQ=255)
       ttggcggTGGGGGGGGGGGGGCGTTTAAAGTTGGCTAATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCaa                                                                                                                                                                                                                                 <  2:702592/233‑1 (MQ=255)
       tgAGCAGTGCGGGCGGGGTTGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACCCTGCCGGACTACCCTGGTTTATTGCCCATTCCTCTTTCCGTTTATTTTGCGAAAAGccca                                                                                                                                                                                                                                 >  1:1147045/1‑237 (MQ=255)
            aGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCTAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAAcc                                                                                                                                                                                                                             <  1:745812/238‑1 (MQ=255)
              tGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTATGCTCGTGATTTCACCGTTACTCTGCCGGCATACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTTTGCGAAAAGCCaaaaaccgg                                                                                                                                                                                                                          >  1:737976/1‑234 (MQ=255)
              tGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCGTCGCGTTTTTCGCCCGTTGTGTCTCCCCTACTCTCCCGGACAACCCCGCGTCAGCGCCAATTCCTCTTACCGCTTTTTTTGCGAAAAAACAAAAcccag                                                                                                                                                                                                                          >  2:878211/1‑236 (MQ=255)
               gcggggggTGTTGCGATTAAAGCTGGCTCATTAATTGCCGTTGTTATTTTGCGACAGACCAAAAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTg                                                                                                                                                                                                                           <  2:1147045/237‑1 (MQ=255)
                        gTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCCTTTTTTTGTCGAAAAGGCCAAACCCGGGGGATTAcc                                                                                                                                                                                                                 >  1:849502/1‑238 (MQ=255)
                        gTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGGTACTCTGCCGGACTACCCCGGTTCAATGCCCATTCCCCTTACCGTTTATTGTGCGAAAAGCCAAAAACTGGGGGATTaac                                                                                                                                                                                                                 >  1:230802/1‑236 (MQ=255)
                        gTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGGCTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTTTGCGAAAAGCCAAAACCTGGGGGATTACct                                                                                                                                                                                                                >  1:614781/1‑238 (MQ=255)
                                 aaaGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGCTGTTTTTGCTCTGGATTTCACCGTTCATCTTCCGGGCTCCCCCGGTTTAGTACCAATTTCTCTTACCGTTTTTTTGGGGGAAAACCAAAAACCGGGGGATTAACTCTCCCGccc                                                                                                                                                                                                      >  2:367769/1‑237 (MQ=255)
                                        gCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGTCGGCTGCGATGTTTCTGCTCGGGATGTCACCCTTAATCTGCCCGCCTACCCTGGTTCATGGCCAATTCCTCTTACCGTTTATTGTGCGTAAATCCAAAAACTGCGGGATTATCTATCCGGCACAACCCCa                                                                                                                                                                                               >  1:1024072/1‑239 (MQ=255)
                                             ttAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTTCTCGTGATGTCACCGTTACTCTTCCGGACTACCCTGGGTCAGGGCCCATTCCTCTTACCCTTTTTTTTGCGAAAAGCCCAAAACTCGGGGATTACCCCTCCGCGCAAAAcccagaaga                                                                                                                                                                                           >  2:369010/1‑232 (MQ=255)
                                              tAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCt                                                                                                                                                                                                                            <  1:356770/205‑1 (MQ=255)
                                              tAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTAt                                                                                                                                                                                                                     <  1:1260723/212‑1 (MQ=255)
                                                      gTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTTTGCTCTTGATGTCACCGTTACTCTGCCGGGCTACCCTGGTTCAATGCCAAATTCTCTTAACCCTTATTTTGCGGAAAGCCAAAAACTGGGGGATTAACTCTCCGGCACaaacaccaaagagggcaaaac                                                                                                                                                                                 >  2:77526/1‑220 (MQ=255)
                                                        gCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGCGGTGCCTACTGGCGGCTCCCGTGTTTTTGTTCGTTCTTTCTCCGCTCCCTTCCCGGACTACCCCGGTTCAGTGCCACTTTCACCTACCCTGTTTTGTTCGAAGAACCCAAACACGGGGGATTTCACCTCCCGGCCACCCCCAAATGGGGGCacaacca                                                                                                                                                                               >  2:154882/1‑233 (MQ=255)
                                                          tttttttGCGACAGCCCACAAACTATAACAGCGATGATTTCCAGTTTGTGTGGGATATTTACGCAAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  2:903062/236‑1 (MQ=255)
                                                          tttttttGCGACAGCCCAACAATATTAAAGGGGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGGGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  2:1162124/236‑1 (MQ=255)
                                                          tttttttGCGACAGAGCAAGAACTAGAGCAGGGATGATTTCAATTTTGTGTGGAAGATTTACGCCAGTAATGATGTGGTGGTGCCTGTGGGGGGCTGTGGTGTTTCTGTTCGTGATGTCACCGTTACTCTGCCGGACTACCCGTGTTCAGGGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTCCCTCTCCGGCACATCCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  2:202196/236‑1 (MQ=255)
                                                          tttttttGCCACAGCCCAACAAATATTACAGCGATTGTTTCCCGTTTTGGTGGAAGATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGGCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  2:665218/236‑1 (MQ=255)
                                                          ttATTTTGCGACAGACCAACAACTATACCAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  1:1044016/239‑1 (MQ=255)
                                                          ttATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  1:383968/239‑1 (MQ=255)
                                                          ttATTTTGCGACAACCCAACAACTATAATCGCTATGGTTTCGAGTTTGTGTGGAATATTTACGCCAAGAATGATGGGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  2:849502/239‑1 (MQ=255)
                                                           tATTTTGCGACAGACCAACAACTATAACGGCGATGATTTCCAGTTTGTGTGGAATATTGACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  1:516030/238‑1 (MQ=255)
                                                                            acaacTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCCGCGGTGTTTCTGCTCGTGATGTCCCCGTTACTCTTCCGGGCTACCCCGGTTCTGTTGCAATTCCTCTTACCGCTTTTTTTGCGGAAAACCCAAAACTGGGGGATTACCTCTCCCGCCCAACCACCGATGCGGGGAACACGCGTTTccacaaaaacccccag                                                                                                                                                           >  2:502383/1‑224 (MQ=255)
                                                                                ttataACAGCGTTGGTTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGGTCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGtttt                                                                                                                                                      <  2:793053/238‑1 (MQ=255)
                                                                                   taACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGGATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTTACCAATACCCCGTCGTTTTCa                                                                                                                                                    >  1:1150666/1‑238 (MQ=255)
                                                                                    aaCAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCCGGGGGATTACCCCTCCGGCCCAACCCCAGATGGGGGCAACTCGATTTTCACCAAAACCCCGCCGTTTTCAc                                                                                                                                                   >  2:809810/1‑238 (MQ=255)
                                                                                        gCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCGCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGGATTACCTCTACGGCACAACCGCAGATGCGGGCAACCCGATTTTCACCAAAACCGCGTCGTTTTTACCTgac                                                                                                                                              >  1:209801/1‑237 (MQ=255)
                                                                                         cGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGGCTACCCTGGTTCAATGGCCATTCCTCTTACCGGTTATTTGGCGGAAAGCCAAAACCTGGGGGATTACCTCTCCGGCACAACCGCCAAAGTGGGGAAATCGATTTTTAACCAAACCACGCGCTTTTCAccctcca                                                                                                                                             >  2:633714/1‑234 (MQ=255)
                                                                                           atgatTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTTTGCGAAAAGCCAAAAACTGGGGTATTACCTCTCCCGCACAACCGCAGATGCGGGCCACTCGATTTTCACCCATACCGCGTCGGTTTAACCTCCACAg                                                                                                                                           >  1:400093/1‑239 (MQ=255)
                                                                                           atgatTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTTGGGGATTACCTCTCCGGCCCAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGGTTTCACCTGcacct                                                                                                                                           >  1:171215/1‑237 (MQ=255)
                                                                                           atgatTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGGGATGTCACCGTTACTCTGCCGGACTACCCCGGTTCATTGCCCATTTCTCTTACCGTTTATTTTTCGAAAAGCCCAAACCTGGGGGATTACCCCTCCGGCCCAACCCCCGATGCGGGCAACCCGATTTTTACCAAAAcccccccggttttccctgccaag                                                                                                                                           >  2:908236/1‑218 (MQ=255)
                                                                                              atTTCCAGTTGGTGTTGAATGTTTCCGCCAATAATGATGTGGTGGTGCTTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCCCCGTTACTCTGCCGGACTACCCGGGTTCAGTGCCAATTCCTCTTCCCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtttt                                                                                                                                                                           <  2:1012302/205‑1 (MQ=255)
                                                                                                      tttGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGGATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTTACCACTACCGCGCCGTTTTTCCCTTCCCAGGGGGTCgggg                                                                                                                                 >  1:1250679/1‑236 (MQ=255)
                                                                                                          tgtgGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACcgcg                                                                                                                                                             <  2:172186/206‑1 (MQ=255)
                                                                                                          tgtgGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACcgcg                                                                                                                                                             >  1:172186/1‑206 (MQ=255)
                                                                                                           ggggATTATTTGCTCCATTAATGGTGTGGTGGTGCGTACTGGGCGCTGGCATGTTTCTGCTCGTTATGGCACCGTTACTCGGCCGAACTGCCATGGTTCAGGGACAATTCCTCTTGCCGGTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGTCGGCGGCGTACAGt                                                                                                                           <  2:233543/237‑1 (MQ=255)
                                                                                                                atatTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCCATACCGCGTCGTTTTCACCCGCCCAGGGCGTCgggtgaccgtttgcg                                                                                                                      >  1:815956/1‑226 (MQ=255)
                                                                                                                   ttttccccAAAAAGGGTGGGGTGGGGCCTACGGGGGGCTGCGATGTTTCTGCTCGTGATGTCCCCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                                                                                                                   <  2:1250679/233‑1 (MQ=255)
                                                                                                                     tttaagggacgggatgTGGGGGGGCCTACTGGGGGGTGGTATTTTTTTGCTCGTGATGGCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCaa                                                                                                                 <  2:840444/227‑1 (MQ=255)
                                                                                                                     ttccccAATAATGGTTTGGTGGGGCCTTCTTGGGGTTGCGATGTTTCTGCCCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCaa                                                                                                                 <  2:220448/235‑1 (MQ=255)
                                                                                                                     ttcGCCAAAAATGATGTGGGGGTTCCTACTGGGGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCaa                                                                                                                 <  2:1150666/237‑1 (MQ=255)
                                                                                                                                  atgTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGAcgcg                                                                                                                    <  1:898958/223‑1 (MQ=255)
                                                                                                                                      ggtggtgCCTACTGGCGGCTGCGATGTTTCTTCCCGTGATGTCACCGTTAATTCGCCCGACTTACCTGGTTCCTTGCCAATTCCTCTTACCGGTTTTTGTGCGAAAAGCCAAAACCTGGGGGTTTACCTCTCCCGCACAACCGCAAATGCGGGAAACCCGATTTTTACCAATACCCCCACCGTTTTCCCCGCCCAAGGGGACGGCGGACCGGTTACGCCCAACgggcacgattttccaa                                                                                                >  2:1193742/1‑225 (MQ=255)
                                                                                                                                      ggtggtgCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCCCAGGGCGTCGGCGTCCAGTTGACGCGCACCGGGACGATTATTCCac                                                                                                >  1:1204919/1‑238 (MQ=255)
                                                                                                                                       gtggtgCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtttt                                                                                                                                                                           <  1:340718/164‑1 (MQ=255)
                                                                                                                                           tgCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGaa                                                                                            <  1:369010/238‑1 (MQ=255)
                                                                                                                                               tACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAaca                                                                                       <  1:950775/239‑1 (MQ=255)
                                                                                                                                                 cTGGCGGGTGCGATGTTTCTGCTCTGGATGTCCCCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTTCCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACg                                                                                     <  1:1332068/239‑1 (MQ=255)
                                                                                                                                                 cTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCCATTCCTCTTACCGGTTATTTTGCGAAAAGCCAAAACCTGGGGGATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTTACCAATACCGCCTCGTTTTCACCCTCACAGCGCGCGCGCGTACAGTTGGCGCCCAAACGTACGAATATTCCCGAGAAAAAAACg                                                                                     >  2:179589/1‑239 (MQ=255)
                                                                                                                                                   ggcgggttgtgttttttttgtCGTTATTTCCCCGTTTATTTTCCGGGCTACCCCGGTTCAGTGCCAATTCCCCTTACCGTTTATTGGGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGt                                                                                   <  2:614781/219‑1 (MQ=255)
                                                                                                                                                     cggcTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTACACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTa                                                                                  <  1:1117550/238‑1 (MQ=255)
                                                                                                                                                         tGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGcac                                                                                                                                                                                                      >  1:1284960/1‑119 (MQ=255)
                                                                                                                                                         tGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGcac                                                                                                                                                                                                      <  2:1284960/119‑1 (MQ=255)
                                                                                                                                                          gCGATGTTTCTGCCCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCCCAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGtt                                                                             <  1:974555/238‑1 (MQ=255)
                                                                                                                                                                        tgTGATGTACCCGTTACTCGGCCGGACTACCCTGGTTCAGTGCCAATTCCCCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGa                                                               <  1:878211/237‑1 (MQ=255)
                                                                                                                                                                          tGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCCACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCTGCCTACAGTTGACCCGCAACGGGACGATTATTCAAGCGAATAAACAGGAATCGTTAgagacaggggggaatt                                                            >  2:1324200/1‑224 (MQ=255)
                                                                                                                                                                              gTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTcggc                                                        >  2:224852/1‑239 (MQ=255)
                                                                                                                                                                                cACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTcggcgg                                                      <  1:1314252/239‑1 (MQ=255)
                                                                                                                                                                                 cccctttccctGCCCGACTACCCTTGTTCAGTGCCAATTCCTCTTACCGTTTATTGTTCGAAAAGCCAAAACCTGGTGTATGACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTGTCACCAATTCCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGt                                                     <  2:209801/230‑1 (MQ=255)
                                                                                                                                                                                 cccGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCCCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGt                                                     <  1:633714/238‑1 (MQ=255)
                                                                                                                                                                                 aCCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCCCAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGCCGGCGTACCGTTGACGCCCAACGGGACGCTTATTCCAGCGAAAAACCCGGGATCGTTAGGCGCCGGAAGGGCTTcgggggg                                                     >  2:1076468/1‑238 (MQ=255)
                                                                                                                                                                                 aCCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGt                                                     <  1:1089826/239‑1 (MQ=255)
                                                                                                                                                                                 aCCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCACCGACTAACCCGGAATCGTTAGGAGCAGTAGGGACTTCGACGGt                                                     >  1:576837/1‑239 (MQ=255)
                                                                                                                                                                                     ttACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAaca                                                                                       <  1:1130966/201‑1 (MQ=255)
                                                                                                                                                                                       aCTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAACACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCCCAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAAAACGGTATCGTTCGAAGCAGTCGGGCATTcggcggcaaggcg                                               >  1:580087/1‑232 (MQ=255)
                                                                                                                                                                                       aCTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTCCGATTATTCCAGCGAATAACACGGTATCGTTAGGAGAAGTAGGGAATTCGGCGGGGGGTcg                                               >  2:427958/1‑238 (MQ=255)
                                                                                                                                                                                       aCTCTGCCGCACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGCATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGCCGTTTTCACCTGCACAGTGCGTCGGCGTACAGTTGACGCGCAACGGGACGCTTTTTCCAGCGACTACCACGGGACCGTTTGGACCAGAAGGGAcctggggggggggtcg                                               >  2:1331836/1‑224 (MQ=255)
                                                                                                                                                                                           tGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTCAGGTTGGCGGGCACCGGTACGGTTATTCACGCGAATAACCCGGATTGGTTAGGGCCAGAATGGACTTCGCGGGAGAGGCTgggg                                           >  2:403975/1‑238 (MQ=255)
                                                                                                                                                                                             ccGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAACGCCAAAACCTGGGGCATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGGCGTTTTCACCTGCACAGGGCGTCGGCGTCCCGTTTACGCGCAACGGTACGATTATTCCACCCAATACAACGGTATTCTTTGGAGCAGAAGGCAATTCGGCGGAGGGGCTggggta                                         >  2:235501/1‑236 (MQ=255)
                                                                                                                                                                                             ccGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGAACGATTATTCCATCGAATAACACGGTATCGTTAGGAGCAGATGGGGATTCGCCAGGGAGTATgggggt                                         >  2:777858/1‑236 (MQ=255)
                                                                                                                                                                                                gACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGGGGGTCTGGGATTAAc                                      >  1:10751/1‑239 (MQ=255)
                                                                                                                                                                                                           ttCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTAt                            <  1:78938/238‑1 (MQ=255)
                                                                                                                                                                                                               gTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCCACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGGGAGTGTGGGCGTAACGGCAAAATATGCaag                       >  2:514284/1‑237 (MQ=255)
                                                                                                                                                                                                               gTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGGGCAGTAGGGATATCGGCGGGGAGGCTGGGagttggtctttataccaactg                       >  2:727076/1‑219 (MQ=255)
                                                                                                                                                                                                               gTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCATTAGGGACTTCGGCGGGGAGTCTGGGATTAACGGCAACTTCTGCCCg                       >  1:1150139/1‑239 (MQ=255)
                                                                                                                                                                                                               gTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTATGCACg                       >  1:874541/1‑239 (MQ=255)
                                                                                                                                                                                                                tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGCTTATTCCAGCGAAAACCCCGGAATCGTTTGGGGCAGTAGGGACTTCGGGGGGGGGGTTGGGTTTAACGGGaaaatttgacgg                       >  2:1192220/1‑229 (MQ=255)
                                                                                                                                                                                                                tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGGGAATAACACCGTATCGTTAGGAGCAGTAGGGCCTTCGGCGGTGGGGTGGGGGTTAACGGCaaaaaagtgacgt                      >  2:629059/1‑229 (MQ=255)
                                                                                                                                                                                                                tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGa                                                                         <  1:570959/188‑1 (MQ=255)
                                                                                                                                                                                                                tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGt                                                     >  1:18031/1‑208 (MQ=255)
                                                                                                                                                                                                                tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCCAATTATGCACGt                      >  1:924643/1‑239 (MQ=255)
                                                                                                                                                                                                                tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGGGAGTCTGGGATTAAAGGCAAATAATGGACga                      >  2:657068/1‑238 (MQ=255)
                                                                                                                                                                                                                    aaTTCCTCTTACCGTTTTTTTGGGCAAAAGCCAAAACCTGGGGTTTTACCCTTCCGGCCAAACCGCAGGTGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTATGCACGTACCg                  <  2:874541/239‑1 (MQ=255)
                                                                                                                                                                                                                    aaTTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                                                                                                             <  1:213205/85‑1 (MQ=255)
                                                                                                                                                                                                                     aTTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGGGAGTCTGGGCTTAACGGCAAATTATGCACGTACCgg                 >  1:883907/1‑239 (MQ=255)
                                                                                                                                                                                                                            ttACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTAACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGGGCAGACGGGCCTTAGGGGTGGAGTCTGGGGTTAACGGCAAATTATTAACGAACCGGGGGGCAg          >  2:1105994/1‑239 (MQ=255)
                                                                                                                                                                                                                              aCCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGGTTAACGGCAAATTATGCACGTACCGGAGGGCCGGt        >  1:69719/1‑239 (MQ=255)
                                                                                                                                                                                                                               ccGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTATGCACGTACCGGAGGGCAGGTg       <  1:224852/239‑1 (MQ=255)
                                                                                                                                                                                                                               ccGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTATGCACGTACCGGAGGGCAGGTg       <  1:511919/239‑1 (MQ=255)
                                                                                                                                                                                                                                    tATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACGGTTGACGCGCAACGGTACGCTTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGCCTTCGGCGGTGAGTCTGGGATTAACGGCCAATTATGACCCAACCGGAGGGCAGGCGATTg   >  1:1066729/1‑238 (MQ=255)
                                                                                                                                                                                                                                    tATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGTGGAGTCTGGGGTTAACGGCAAATTATGCACGTACCGGAGGGCAGGGGAcctc  >  2:368660/1‑236 (MQ=255)
                                                                                                                                                                                                                                    tATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGGGGGTCTGGGATTACAGGCAAATAAAGCAGGGACCGGGGGGCAAGGGACGGc  >  2:80508/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            
ACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACACGGTATCGTTAGGAGCAGTAGGGACTTCGGCGGTGAGTCTGGGATTAACGGCAAATTATGCACGTACCGGAGGGCAGGTGACTGC  >  NC_000913/4549199‑4549665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: