Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,631,506 | A→G | 12.2% | E258G (GAG→GGG) | slt → | lytic murein transglycosylase, soluble |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,631,506 | 0 | A | G | 12.2% | 206.3 / 3.2 | 84 | E258G (GAG→GGG) | slt | lytic murein transglycosylase, soluble |
Reads supporting (aligned to +/- strand): ref base A (23/49); new base G (9/2); total (33/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.43e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATA > NC_000913/4631273‑4631730 | tCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATg < 1:917683/239‑1 (MQ=255) aacgcgGGCGTTTTGGGAGCGTATCCGGTTGGGGATGAAAGGGGGTAACGCGGGCCGGGTAACAGTGGTTGCAGGGCAGATGCCTGCCGATTACCAGACTAGCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATg < 2:1108609/237‑1 (MQ=255) cGCTGGCGTTTTTAGAGGGGTTCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCa < 2:437721/239‑1 (MQ=255) ggttgCGTATTTAGAGCCTTTCCCGTGCGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATGCGGTCCTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCAc < 1:402946/235‑1 (MQ=255) ggCGTATTTAGGGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACgg < 1:675990/238‑1 (MQ=255) tttAGAGCGTATCCGTATGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCtgatg < 1:908692/238‑1 (MQ=255) agCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCCGTGTGGCGCGGCCGGAGGCTTAGaaatgaaggcgtatgtatcca > 1:817621/1‑220 (MQ=255) tATCCTTTTGGCGATGAAAGCGGGTAACCACGGCCTGGTAACGGTGCTGGGAGGGCAGATGCCTGCCGATTTCCAGACTTCCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGc < 2:817621/239‑1 (MQ=255) aTCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGACCAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTTCCAGTGTGGCGCGGCCGGCTGCTGTGAATGCACggtttctagtcccctccc > 1:1048051/1‑222 (MQ=255) aTCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGGTTGCCAGTGGGGACCGGCCGGATTCTTGGGATTCACGGCTAGAGATCCCCTcagt > 1:505416/1‑236 (MQ=255) cctCTGGCGGTGAAAGGGGGTAACCCAGGCCTGGTAACAGTGGTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCATCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGc < 2:1067672/237‑1 (MQ=255) cGTCGGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGc < 1:26638/239‑1 (MQ=255) tGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCCATTTTACCCGTCAAATGGCGCGGGGGGGCGTTT‑CCCGTGGGGGGCGGGCGGGGGCTGGGAAAGGACGGCCGGTGAGCCCCCCGCGTTCCCac > 2:26638/1‑238 (MQ=255) gcaaGCGGGTAACCCAGGCCTGGTAACAGGGCTGGCAGGGCAGATGCCTTCCGATTACCCGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATGCGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag < 2:99104/236‑1 (MQ=255) gAAATCGGGTAACACAGGCCTGGTAACAGGGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag < 1:185544/238‑1 (MQ=255) gAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag < 2:970097/238‑1 (MQ=255) aaGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcagca < 1:66517/238‑1 (MQ=255) aaccccggCCTGGGAACAGTGCTGGCAGGGGAGATGCCTGCCGATTACCAGGCTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATg < 2:1115309/232‑1 (MQ=255) ggCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCa < 1:395002/239‑1 (MQ=255) gCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGGGAATGCACGGCTGAGGATCCCATCGCTTTCCCAGGGGCGGACGCTTAAAGAAGATCAg > 1:381460/1‑239 (MQ=255) gTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGAGCGGCAGGATGCTGGGAAGGCACGGCTGCTGATCCCATCGCTTGCCCAGGCGGCGAAGCTTAATGAAGATCAGGTTCa > 1:296236/1‑239 (MQ=255) gTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGGGGCGTTTGCCAGCGTGGGGCGGCCGGGTGCTGAGAATGGACGGGGGGAGTGCCCCTCGCGTGCCCGGGCGCAGCAGCGTAAATAAGATAAAAttta > 2:594458/1‑237 (MQ=255) aaCAGGGCGTGCAGGGCAGATGCCCGCCGTTTACCAGACTATCGCCACGGCAATCATTTCACGGGCGAACAACTCTAATACGGTACTGGCCTGCGCGCGTACAACGGGCGGGACCGATTTTACCCGTCAAATGGCG‑GCGGGGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAgg < 2:812497/239‑1 (MQ=255) aaGTGCTGGCAGGGCAGATGCCTGCCGATTGCCAGACTTACGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAg < 1:1222710/238‑1 (MQ=255) gTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCa < 2:1046331/177‑1 (MQ=255) gTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCa > 1:1046331/1‑177 (MQ=255) tGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGGGGCGCGGCAGGAGGCTGAGAATGCCCGGCGGGTGCTCCCCTCTCCTGCCCAGGCCCAGCCGCTTaaagtaaacaaaatttaagggccg > 2:1147304/1‑217 (MQ=255) ggtGGCAGGGCCGGTGCCGGCCGATTACCAGGCTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGGTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAg < 2:858656/233‑1 (MQ=255) gCTGGCAGGGCAGATGCCTGCCGATTCCCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAg < 1:594458/235‑1 (MQ=255) tGGCAGGGCAGATGCCTGCCGATTACCAGACTACCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTgcg < 1:980455/238‑1 (MQ=255) tgccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCCCCTGGc > 1:853615/1‑239 (MQ=255) tgccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGAGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCCGTTGCCCGTGTGGAGCGGCCGGAGTCTGGGAAGGCAGGGGCGATGGTCCCCCCGCTAGCCCAGGCGGCGACGCGTAATAAAGGACAGGACGAGGAGATGCGCGATAACGTCGccgcgc > 2:175761/1‑235 (MQ=255) cTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCaa < 1:1304646/238‑1 (MQ=255) cGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGGGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCCGGGGTTTGATTGGCAACGagg > 1:137057/1‑237 (MQ=255) cGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGTTCCCATCGCTTTCCCAGGCGCAGCAGCTTAATGAAGATCAAATTCAGGGGCTGCGCGGTTTCGCGGCCTGGGGTTTGTTGGGCAACGTTGtaccc > 1:252959/1‑235 (MQ=255) ggCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAAAGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGGAACcg > 1:35858/1‑239 (MQ=255) aacCCTAATGCGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGa < 1:923975/239‑1 (MQ=255) ccttagtcgggtgtggccTTCGCGGGTAGAACTGGCGCGACCGATTTTTCCCGTCAAATGGCG‑GCGGGGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAAGGCACGGGTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCGGCGCGAGATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACTAGCAGGTGAAATGGCGCGATGa < 2:1289631/223‑1 (MQ=255) ccTAATACGGTACTGACCTTCGGGCGTACAACTGGCGCGACCGATTTTACCCGGCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATg < 1:1202201/238‑1 (MQ=255) cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGGGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATTGGCAACGATGTCACCGCCGAGCAGGCGAAATGGCGCGATGa > 1:1320624/1‑238 (MQ=255) cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGGGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCGGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGGTATCCTCGCCCGGCGTTTGTAGGGTAACCATGTCACCGACGGCCAGGCGAAACGGGGCGGTggc > 2:1091570/1‑237 (MQ=255) tttttgggTTCTGTCCTTTGCGCGTACCACTGGGGCGAGCGATTTTACCCGGCAAATGGCG‑GCGGGGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCAGCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACg < 2:381460/233‑1 (MQ=255) aTACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCCGGGGGGCGCGGCCGGCTGCTGGGGATGCAAGGCTGGTGATCCCATCCCTTGCCCACGCGCAGCAGCTTAATGAAAATCAGATTACGGAGCTTGGCGATATGCGAGCCTTGCGTTCGATTGGGAACGATGTCACCGACACGCAGGCGAAATgggcgggggagacc > 2:1248783/1‑227 (MQ=255) tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGGTGCTGGGAATGCCCGGCTGATTATTCCCTCGCTTTCCCAGGCGCAGCAGCTTAATGAAGATCCGATTCAGGGACGGCGCGATTTGGTCCCCTGGCGGTTGAGGGGGAACGAGGTCACCCCCGAGCAGGCGCAAAgggggggggaagcccaaaag > 2:773115/1‑221 (MQ=255) tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATTCACGGCTGATGATCCCATCGCTTGCCCCGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAAGTGCGCGAAATCGTCCCCCTGCGGTTGATGGGCAACGATGTCACCGGCGGGACGGCGAAAAGGCGCGaaaaacgcactaag > 2:1202201/1‑226 (MQ=255) tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATAtc < 1:897361/170‑1 (MQ=255) tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGGGCACGCGAAATGGCGCGATGACGCCCTTATg > 1:259305/1‑239 (MQ=255) tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGGGGGATGACGCCATTTTg > 1:439804/1‑239 (MQ=255) tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTAt > 1:107429/1‑238 (MQ=255) tgcccttcggctgTACAACGGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGACCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcgc < 2:249655/227‑1 (MQ=255) tGACCTTCGCGGGTACAACGGGCGCTGCCGATTTTCCCCGTCAAATGGCG‑GCGGTGGCGTTCGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcgc < 1:453791/239‑1 (MQ=255) tGACCTTCGCGCGTACAACTGGCGCGACCGATTTTGCCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcgc < 1:228421/239‑1 (MQ=255) gtatcttggcgggttcacttGGCGGTACCGATTCTGACCGTCAAAGGGCG‑GCGGTGGCGTTTGCCAGGTTGGCGCGGCAGGATGCGTAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcga < 2:1261/220‑2 (MQ=255) aCCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGGGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCCGCAGCTTAATGAAGATCAGATTCAGGAGCTGCCCGATATCGTCGCCTTGCGTTTGATGGGAAACACAGTCACCGACGGGACGGCGAAATGGCGCGGTTACGCCATTTTgcacc > 1:658785/1‑235 (MQ=255) cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGGGTGGCGCGGCAGGATGCTGAGAATGCACGGCCGATGATCCCATCGCTTTCCCAGGCGCAGCAGCTTAATGAAGATCCGATTCAGGAGGTGCCCGATAACGTCGCCTGGCGTTTGATGGGGAACGATTTCACCCACGGGCAGGCGCAAAGGGGGGATGagcccccaatgggcccgcaac > 2:1372261/1‑219 (MQ=255) cgTACACGTGGCGCGCACGATTTTCCCCGTCAAAGGGCG‑GCGGTGGCGTTTGCCAGTGGGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAAGGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTAc < 2:252959/239‑1 (MQ=255) gtgcAACTGGCGGGACCGATTTTCCCCGTCAAATGGCG‑GCGGTGGCGTTTGCCCGGGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTAc < 1:405815/235‑1 (MQ=255) gTACAACGGGGGGGGACGATTTTACCCGTCAAAGGGGG‑GCGGGGGGGTTTGGCAGGGGGGCGGGGCAGGAGGCTGAAAATGCACGGGTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGAGATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTAc < 2:1048051/238‑1 (MQ=255) tACCACTGGCGCGACCGATTTTACCCGTCAAAGGGCG‑GCGGTGGGGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACt < 2:658785/238‑1 (MQ=255) cAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTc < 1:1132345/238‑1 (MQ=255) gACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGGGGGGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGaa < 1:551362/238‑1 (MQ=255) gccGGTTTTCCCCGGTAAATGGGG‑GGGGTGGCTGTTGCCAGGGGGGCGCGGCAGGATGCTGAGAAGGCCCGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACg < 2:107429/238‑1 (MQ=255) cgcGATTTTCCCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACg < 2:35858/237‑1 (MQ=255) ccccgttttACCCGTCAAATGGCG‑GCGGTTGCGTTTGCCAGGGTGGCGCGGCAGGATGCTGAGAATGCCCGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACg < 2:296236/234‑1 (MQ=255) ccGATTTTCCCCGTCAAATGGCG‑GCGGGGGCGTTTGCCAGGGGGGCGCGGCAGGATGCTGAGAATGCCCGGCTGATGAATCCATCGCGTGCCCAGGCGCCGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATAGCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACg < 2:259305/238‑1 (MQ=255) ccGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGGGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGaa < 1:1147304/239‑1 (MQ=255) ccGTAAAATGGCG‑GCGGTTGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGGTGATGGTCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGAATCAGGAGCTGCGCGATATCGTCGCCTGGCGTGTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGaa < 1:1377523/239‑1 (MQ=255) gttaaaTGGGG‑GGGGTTGGGTTTGCCAAGGGGGCGGGGCAGGATGCTGAGAATGCACGGCTGGTGGTCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGAACAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATg < 2:137057/236‑1 (MQ=255) tCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTTAACGCCGGTTACGAATg > 1:798352/1‑238 (MQ=255) ggg‑gcggTGGCGTTTGCCAGTGGGGCGGGGCAGGATGGTGGGAAGGCACGGTTGATGAGCCCATCGCTTGCCCAGGCGCAGCATCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTgg < 1:634240/237‑1 (MQ=255) gcggTGGCGTTTGCCAGTGTGGCGCGGCAGGGTGCTGAGAATGCACGGCTGATGATCCCATCGCTTTCCCAGGCGCCGCAGCTTAATGAAGATCAGATTCCGGAGCTTCGCGCTATCGTCGCCTGGCGTTTTGTGGGCAAAGAGTTAACCCACGGGCAAGCGAAAAGGGGCGATGACGCCCTGATGGGCTCGCAATCTTCTTCGCTTTTTGAACGcccggtgagaaagggggtttgcc > 2:828901/1‑217 (MQ=255) gcggTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATccc > 1:744972/1‑59 (MQ=255) gcggTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAAAGCCGTGTACGAATGGGGCTTGGCa > 1:1361862/1‑238 (MQ=255) ggTTGCGTTTGCCAGTGGGGCGAGGCAGGATTCTGAGAATGCACGGCTGATGGTCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCAcc < 1:204090/238‑1 (MQ=255) ccAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTcgc < 1:107864/239‑1 (MQ=255) gtgtGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATg < 1:805002/129‑1 (MQ=255) gtgtGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATg > 2:805002/1‑129 (MQ=255) tgtgGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCCCGGc > 1:231683/1‑238 (MQ=255) tgggCGCGGCAGGATTCTGGAAATGCCCGGCTGATGTTCCCCTCGCTTTCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCt < 2:439804/237‑1 (MQ=255) gtgGCGGGGCAGGATGCTGAGAAGTCCCGGTTGGTGATCCCATCGCTTGCCCAGGCGCCGCAGCTTAATGAAGATCAGATTCATGAGCTGCGCGATATCGGCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCgg < 1:453088/236‑1 (MQ=255) tgGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCCATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCCCCGCCGACTGTCGCGGCCTg > 1:719196/1‑239 (MQ=255) cgGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCCCGATAACGCCATTATGCCCTCGCCACCTCCTTCGCTTATTGAAGCCCGGGTACGAATGGGGCTTGGCCACGGCGCTCGCCCCGGCCCGaaaa > 2:1045495/1‑236 (MQ=255) cAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGa > 2:355210/1‑146 (MQ=255) cAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGa < 1:355210/146‑1 (MQ=255) | TCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCG‑GCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATA > NC_000913/4631273‑4631730 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |