BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,638,877	T→G	11.0%	G233G (GGT→GGG)	creD →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,877	0	T	G	11.0%	411.8 / 3.0	169	G233G (GGT→GGG)	creD	inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (64/76); new base G (26/2); total (91/78)
Fisher's exact test for biased strand distribution p-value = 1.86e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TGGTGAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTT  >  NC_000913/4638642‑4639111

tggtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGGTCTTTCTGTGGTGCCCGGCGGGCGGAATAGCGAAAAGACCTTAAACA‑ACAAAGGGCCCCATCCCAGGTTTTTTGGGGaa                                                                                                                                                                                                                                            >  2:221098/1‑238 (MQ=255)

  gtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCCGGTTTTTTGGGGAttt                                                                                                                                                                                                                                          >  1:421866/1‑239 (MQ=255)

  gtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAACTGAATATGGCCCTGAATTTAAGCGGTACCGGCGACTTTTCATGGGTGCCTTGCGGGCCGAAAAGCGAAAAGACCTTTACCC‑ACACATGGCCGCCTCCACGTGTTTTTGGGGAttt                                                                                                                                                                                                                                          >  2:827594/1‑239 (MQ=255)

       aGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGGAGCAGAACCTGAAGCTGAAAATGGCCCTGAATTTAAGCGGTAACGGCGAACCTTTTGTGGTGCCCGGCCGGCTGAAAAGGGAAAAGACCTTTACCA‑CCCACGGGCCGCCTGCCCGGTTTTTTGGGGGTTTTTTAc                                                                                                                                                                                                                                     >  2:716466/1‑239 (MQ=255)

       aGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTTTGGTGCCTGGCGGGCGGAATAAGGAAATGACCTTAACCA‑GCAACATGACGCATCCCGGGTTTTTAGGGGATTTTTTa                                                                                                                                                                                                                                      >  1:474937/1‑238 (MQ=255)

           ccTGAAGTTAACCGAAGCGCCGTTG‑CCATTGAACCCGGGCCCGGGTTAGGGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGc                                                                                                                                                                                                                                 <  2:421866/239‑1 (MQ=255)

            cTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATACCAGGTTTTTTGGTGATTTTCTACCAGcc                                                                                                                                                                                                                                >  1:1250137/1‑239 (MQ=255)

             tGAAGGTAACGTGA‑CGGGGCGGCCCATTGAACCCGGAACCGGGTTAGGGCAAGGCGGGCAGGGCGGTCATATCCCTTTTCCTGAAGGGGACGGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAGTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTGCTACCAGCCa                                                                                                                                                                                                                               <  2:1107543/239‑1 (MQ=255)

                     acggaa‑cggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGGACCGGGGATCTTTCTGTGGTGCCTCGCGGGCGTGATAACGGAATGGCCCTAACCA‑GAAACAGGCCGCATCCCACTTTTTTTGGGGAtttttattcacaccaaacggga                                                                                                                                                                                                                       >  2:1132607/1‑221 (MQ=255)

                                               tggCCCCGGGGTAGAGCTAGGGGGGCGGGGCGTGTATTTCCCTTTCACTGGAGGGGACTGGCGGAAGCATAACCTGAAGCCGAATATGGCCCGGGATTTAAGCGGGACCGGCGATCTTTCTGGGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGGTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            <  1:146441/238‑1 (MQ=255)

                                               cggacctgggTTAGAGGAGGGTGGGCAGGGTGTGCATATCCCGTTTCCTGAAAGGGACTGGGGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGGGTGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            <  2:339302/232‑1 (MQ=255)

                                                ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGGAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCCGTTTTTTAGGGGATTTTCTACCCGCCAAACGGG‑AA‑GGTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            >  1:924053/1‑238 (MQ=255)

                                                ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTAACGGCGATCTTTCTGTGGGGCCTGGCGGGCGTAATAACGAAATGACCTTAACCC‑GCAAACGGCCGCCTCCCAGTTTTTTTGGGGGTTTTTTACCAGCCCAACGGG‑AA‑GTTAGCGAGTCAGGGTTTTAGgcgg                                                                                                                                                                                            >  2:1296522/1‑237 (MQ=255)

                                                ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAAAATGGCCCTGAATTTAAACGGTACCGGCGATCTTTTTGTGGGGCCTGGCGGGGGGAATAACGCAACGACCTTAACCC‑GCACCTGGCCGCCTCCCCGCTTTTTTGGGGATTTTCTACCCGCCAAACAGG‑AA‑GGTAGCggggaggagtttccatgtc                                                                                                                                                                                            >  2:1102213/1‑220 (MQ=255)

                                                ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATACCTTTACCTGAAGGGGACTGGCGGGAGCCGAACCTGAAGCTGCATATGGCCCTGAATTTAAACGGTACCGGGGATCTTTCTTTGGTGCCCTGCGGGGCTAATAGCGAAATGGCCTTTACCAGGCAAC‑GGGCGCATCCCCGGTTTTTTGGTGAGTTTTTCCCCACCAAACGGGCAG‑GTTACGGAGGCAGGTTTTAGGgcg                                                                                                                                                                                             >  2:892659/1‑238 (MQ=255)

                                                      gggTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCa                                                                                                                                                                                     <  1:1277048/239‑1 (MQ=255)

                                                        gTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCa                                                                                                                                                                                                                                   <  1:578168/193‑1 (MQ=255)

                                                           agggCAAGGCGGGCAGGGCGGGAATATCCCTTTGCCGGAAGGGGACTGGCGGAAGCAGAAACTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAagc                                                                                                                                                                                 <  1:1289309/235‑1 (MQ=255)

                                                                 aGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAAAGGG‑AG‑AGTACCGAGGCCGGGTTTTCGGGGCCCCGGCAAAGCCGCCggg                                                                                                                                                                          >  2:26424/1‑238 (MQ=255)

                                                                  ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGGAGCCGAACCTGAAGCTGAATATGGCCCTTGATTTTAGCGGTACCGGCGATCTTTCTGTGGTGCCCGGCGGGAGTAATAGCGAAATGAACTTAACCA‑GCAACTGGCCGCCACCCCATTTTTTCTGTGATTTTCTCAACTCCAAACGGG‑AA‑GCAAAAGAGGCAAGTTTTTCGGCGCACCGGGAAaacaacagggg                                                                                                                                                                         >  2:621672/1‑232 (MQ=255)

                                                                  ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTTTGTGGTGCCTGGCGGGCGGAATAACGAAAGAGCCTTAACCA‑GCAACTTGCCCCCTCCCAGGTTTTTTGGTGATTTTTTACCAACCAAAAGGG‑GA‑GGTAGCGAGTCGGGGTTTTTTGGGCACTGGCAAAACAACTgggt                                                                                                                                                                         >  2:1094443/1‑237 (MQ=255)

                                                                  ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTTGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCCACTGGCCGCATCCCCGTTTTTTTGGCGGTTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGGTTTCCGGGGCACTGGCCAAACAGCGGGtt                                                                                                                                                                         >  1:638111/1‑239 (MQ=255)

                                                                   gggggCAGGGGGTGCATTTCCCTTTGCCTGAAGGGGACTGGGGGAAGCAGAACCGGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGt                                                                                                                                                                          <  2:658775/235‑1 (MQ=255)

                                                                    cGGGCAGGGCGTGCATAGTCCCTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGtt                                                                                                                                                                         <  1:8206/237‑1 (MQ=255)

                                                                       gCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAGCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCt                                                                                                                                                                     <  1:1039746/238‑1 (MQ=255)

                                                                       gCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGGAAACGCGAAATGACCTTAAACA‑GCAACCTGCCCCATGCCAGTTTTTTAGGTGATTTTCTTCCCCCCACAAAGG‑TA‑GGTACCCAGTACGGTTTTCCGGCGCACCGGCAAaccaaacggttttgtt                                                                                                                                                                    >  2:91741/1‑227 (MQ=255)

                                                                         cgggCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCtaa                                                                                                                                                                   <  2:1199941/237‑1 (MQ=255)

                                                                         aGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCtaa                                                                                                                                                                   <  1:207921/238‑1 (MQ=255)

                                                                          gggCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑CCAACTGGCCGCATCCCCGTTTTTTTGGGGATTTTCTACCCGCCAAACGGG‑AAGGTTAGCG‑GGCAGGTTTTTAGGGGCACCGGCAAAACAGCAGGTTTgtgcaaa                                                                                                                                                                 >  2:203813/1‑233 (MQ=255)

                                                                                   tatCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTg                                                                                                                                                        <  1:300029/239‑1 (MQ=255)

                                                                                     tCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGGCTCCCAGTTTTTTAGGGGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTACGGCGCCCTGGCAAAGCCGCCGGTTTGCGAATAATCTCGGTGAg                                                                                                                                                      >  1:155269/1‑239 (MQ=255)

                                                                                          ttACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑GA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAACAATCTCGGTGAGCGttt                                                                                                                                                 >  1:640434/1‑239 (MQ=255)

                                                                                          ttACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGGGATTTTCTACCAGCCAAACGGG‑AA‑GTTAACGAGTCCGGTTTTCAGGGGCCCTGGCAAACAAGCTGGTTTGTTAATAATCCCGGGTAGGGttt                                                                                                                                                 >  2:153449/1‑239 (MQ=255)

                                                                                          ttACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑ACAACTGGGCGCCTCCCCGGTTTTTAGGGGATTTTTTACCAGCCAAACGGG‑GA‑GTTAGCGAGTCCGGTTTTCCGGGGCACTGGCCAAGCAGCTGGGTTGCTAATAAACTCGGTCAGCGttt                                                                                                                                                 >  2:300029/1‑239 (MQ=255)

                                                                                           tACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGtttt                                                                                                                                                >  1:1009372/1‑239 (MQ=255)

                                                                                               ggAAGGGGAGGGGCGGAAAGAGAACTGGAAGCTGAAATGGGGCCGTAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGGGGGCGTATGAGCGAAATGACCTTAACCC‑GCAAGTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCTCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCtt                                                                                                                                            <  2:783337/238‑1 (MQ=255)

                                                                                               ggAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCAGTGAGCGTTTTGCtt                                                                                                                                            <  1:376461/238‑1 (MQ=255)

                                                                                                gAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTAGCCGCATCCCCGTTTTTTTGGTGATTTTCTACCAGCCAAAAGGG‑CA‑GTTACCGAGTCAGGGTTTCAGGCGCCCGGGCCAAACAGCTGGTTTGCTTATAATATCGGTGACGGTTTTGTTTc                                                                                                                                           >  2:1056974/1‑239 (MQ=255)

                                                                                                 aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATTGCCCTGAATTTAAGCGGTACCGGAGATCTTTCTGTGGTGCCTGGCGGGGGTAATAGCGAAATGACCTTAAACA‑GCAACCGGCCGCATCCCCATTTCTTAGGGGATTTTCTACCCGCCCAACGGG‑CA‑GTTATCGGGTCAGGGTTTCCGGCGCACCGGCAAAACACCCGGGTTGCCACAAATTCCGGGGGGGGTTTttcttcc                                                                                                                                          >  2:994559/1‑238 (MQ=255)

                                                                                                 aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCCACTGGCCGCCTCCCCGTTTTTTTGGGGGTTTTTTTACACCCAAACGGG‑AA‑GTTAGCAGGCCAGGGTTTTAGGGGCCCTGGCAAAACAGCCGGGTTGCTAATAACCCCGGGGGGCGGTTTTTTTCa                                                                                                                                          >  2:399170/1‑239 (MQ=255)

                                                                                                 aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCa                                                                                                                                          >  1:770596/1‑239 (MQ=255)

                                                                                                 aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGGGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGAAAAAAAAGCGGGTTGTTAAAAATCTCGGGGAGCGGTTTGCTTCa                                                                                                                                          >  2:291706/1‑239 (MQ=255)

                                                                                                 aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGGAATAGCGAAATGACCTTAACCA‑GCAAATGGCCGCATCCCAGTTTTTTAGGTGATTTTTTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCCGGTTTTCAGGGCCACCGGCAAAGGAGCCGGTTTTCTAAAAATTCCGGTGGGGGgtttgtttaa                                                                                                                                          >  2:1031020/1‑237 (MQ=255)

                                                                                                  aGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCa                                                                                                                                          <  1:221098/238‑1 (MQ=255)

                                                                                                       aCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGtt                                                                                                                                                                         <  2:1015860/202‑1 (MQ=255)

                                                                                                       aCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGtt                                                                                                                                                                         >  1:1015860/1‑202 (MQ=255)

                                                                                                           gCGGAAGCAGCACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGCTCTTTCTGTGGTGCCTCCCGGGCGTAATAGCGCAATGACCTTAACCA‑GCAACCGGCGGCATCCCAGTTTTTTTGGTGTTTTTCTCCCAGCCAAACGGG‑CA‑GTTAGCGAGTCAGGTTTTCACGCGCACCGGCAAAGCAGCTGGCTTGCTTATAATCTCGTCGACCGCTTCGCTTCatgacatcgt                                                                                                                                 >  2:307516/1‑229 (MQ=255)

                                                                                                                aGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGGGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCCCTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGGGCGTTTTGCTTCAGGCAATGATACCCGc                                                                                                                           >  1:588655/1‑239 (MQ=255)

                                                                                                                     aaCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTAGCTAATAATCTCGGTGAGCGCTTTGCTTCAGGCAATGAGAACGGCTGGGa                                                                                                                      >  1:149573/1‑239 (MQ=255)

                                                                                                                               tGAATATGGCCCTGAATTTAAGCGGTACCGGGGATCTTTCTGTGGTTCCTGGCGGGCGTAATCGCGAAATGACCTTAACCA‑GCAACTGGCCGCAACCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCg                                                                                                            <  1:1366572/239‑1 (MQ=255)

                                                                                                                               tGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTcc                                                                                                              <  1:818321/237‑1 (MQ=255)

                                                                                                                               tGAATAGGGCCCTGAATTTAAGCGGTTCCGGCGATCTTTCTGGGGTGCGTGGAGGGCGTAATAGCGAAATGACCTTAACCA‑GGAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTcc                                                                                                              <  2:1250137/237‑1 (MQ=255)

                                                                                                                                 aaTATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCCGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCCCTGGCAAAGCAGCTGGTTTGCTAATCATCCCGGTGCGCGTTTTGCTTCAGGACATGAACCCGGCTGGGAAAACATCCCGGc                                                                                                          >  2:1081710/1‑239 (MQ=255)

                                                                                                                                  atatGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCCAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCCATGGCCAAGCAGCTGGTTTGCTAATAATCTCGGTGGGCGTTTTGCTTCAGGAAATGATACCGGCTGGGAAAAATTccacgcg                                                                                                         >  2:281909/1‑234 (MQ=255)

                                                                                                                                   tatGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTACCGGCGt                                                                                                        >  1:619547/1‑239 (MQ=255)

                                                                                                                                        cccTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGCCGGGGAAAACTTTCCGGCGTTTAGc                                                                                                   >  1:298200/1‑239 (MQ=255)

                                                                                                                                          cTGAATTTAAGCGGGACCGGCGATCGTGCTGTGGGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAAGCACGGTGAGCGTTTTGCTTCAGGCAAGGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGt                                                                                                 <  1:220095/239‑1 (MQ=255)

                                                                                                                                           tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCgg                                                                                                 >  1:868119/1‑237 (MQ=255)

                                                                                                                                           tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGAGt                                                                                                 >  1:26546/1‑238 (MQ=255)

                                                                                                                                           tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGCCAATGATACAGGCTGGGAAAACTTCCCGGCGTTTAGAGTc                                                                                                >  1:189022/1‑239 (MQ=255)

                                                                                                                                           tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GGTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTTCCGGCGTTTGGCGTc                                                                                                >  1:689074/1‑239 (MQ=255)

                                                                                                                                             aaTTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAACCGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCCGGCAAGAGTACCGGCTGGGGAAAATTCCCCGCGGTTAGCGTCga                                                                                              >  2:386445/1‑238 (MQ=255)

                                                                                                                                              aTTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCC‑AACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCa                                                                                             <  1:716466/238‑1 (MQ=255)

                                                                                                                                                       gTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTTCCGGCGTTTAGCGTCGCAGTAacta‑c                                                                                    >  1:511270/1‑238 (MQ=255)

                                                                                                                                                       gTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTCCAGGCAATGATACCGGCTGGGGAAAATTCCCGGCGTTTACCgctcaagtaaaccc                                                                                     >  1:1138576/1‑225 (MQ=255)

                                                                                                                                                         aCCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTTCCGGCGTTTAGCGTCGCAGTAACGA‑CGcc                                                                                 >  1:977557/1‑239 (MQ=255)

                                                                                                                                                           cGGCGTTTTTTCTGGGGTGCCTGGGGGGCGAAATAGCAAAAGGACCTTACCCA‑GCAACGGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTGGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAg                                                                               <  2:1009372/239‑1 (MQ=255)

                                                                                                                                                            ggCGATCTTTCTGGGGTGCCTGGCGGGCGTAATAGCGAAATGCCCTTAACCA‑GCAACTGGCCGGATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCTCAGTAACGA‑CGCCAGc                                                                              <  1:985276/239‑1 (MQ=255)

                                                                                                                                                                  cTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTCATAATCTCTGTGGGCGTTTTGATTCAGGCACTGATACCGGCCGGGAAAACTTCCCGGCGTTTAGCGTCTCAGGAACGA‑AGACAGCCGATCa                                                                        >  2:186175/1‑239 (MQ=255)

                                                                                                                                                                  cTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAAACTCGGGGAGCGTTTTGCTTCAGTCACTGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCCGTa                                                                                          >  1:1097955/1‑222 (MQ=255)

                                                                                                                                                                   tttCTGGGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCaa                                                                       <  1:1132607/239‑1 (MQ=255)

                                                                                                                                                                       tgtgGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATAcc                                                                   <  1:759394/239‑1 (MQ=255)

                                                                                                                                                                           gtgCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGGAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAAtt                                                               >  1:1348170/1‑239 (MQ=255)

                                                                                                                                                                           gtgCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGGGTCGCAGTAACGA‑CGCCAGCCGTCCAAAACCaact                                                               >  1:516174/1‑237 (MQ=255)

                                                                                                                                                                            tgCCTGGCGGGCGTAATAGCGAAATGGCCTTAACCA‑GCAACTGGCCGGATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAAtt                                                               <  1:399170/238‑1 (MQ=255)

                                                                                                                                                                            tgCCTGGCGGGCGGAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAAAGATACCGGCCGGGAAAAACTCCCGGCGTTTCTGTTCGCATGAACAA‑CGCCaaaccacacaaaacaacaa                                                              >  2:1239981/1‑221 (MQ=255)

                                                                                                                                                                             gCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCaaa                                                                                                                                                                                                                             <  1:510151/82‑1 (MQ=255)

                                                                                                                                                                             gCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCaaa                                                                                                                                                                                                                             >  2:510151/1‑82 (MQ=255)

                                                                                                                                                                              ccTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGCCGTTTAGCGTGCCCGTAAAGA‑CGCCAGCCGATCAATACCAATTa                                                              >  1:188969/1‑237 (MQ=255)

                                                                                                                                                                              ccTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGC‑GAGACAAGCGCCACCCGATCAAAACCAATTaaa                                                            >  2:57586/1‑238 (MQ=255)

                                                                                                                                                                                 ggcgggcgTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTTGCGTCGCAGGAAAGA‑CGCCAGCCGAACAAAAACaaaaaaaaga                                                         >  2:1211238/1‑231 (MQ=255)

                                                                                                                                                                                         ttattcgctaaTGCCCTTAACCC‑GCAATTGGGCGCATTCCAGTTTTTTTGGTGATTTTCCACGAGCCAAACGGG‑AA‑GTTAGGCAGTCGGGGTTTCAGGCTCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGa                                                 <  2:128618/230‑1 (MQ=255)

                                                                                                                                                                                           ttaggaaattgcctttAACCA‑ACAACTGGCCGGCTTCCAGTTTTTTGGGGGATTTTCTGCCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACt                                               <  2:636080/225‑1 (MQ=255)

                                                                                                                                                                                           aTAGGGAAATGACCTTACTCA‑TGATCTGGCCGCTTCCCATGTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTGGCGAGTCAGGTTTTCAGGCGCACTGGAAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACt                                               <  2:608365/239‑1 (MQ=255)

                                                                                                                                                                                                gAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTa                                          <  1:1307159/239‑1 (MQ=255)

                                                                                                                                                                                                 aaagccccTTAGCCT‑GCCACGGGCCCGACCCCAGTTTCTTGTGTGATTCTCAACCAGCCAAACGGG‑AA‑GTTAGGTGGTTAGGTTTTCAGGCACACTGGCAAAGCAGCGGGTTTGCTAATAATCCCGGTGAGCGTTGTGCTTCAGGCAATGATACCGGCTGGGAAAACCTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTa                                          <  2:516174/232‑1 (MQ=255)

                                                                                                                                                                                                  aaTGCCCTTAACCA‑GAAAATGGCCGCAACCCAGTTTTTTGGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTGTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:1239981/239‑1 (MQ=255)

                                                                                                                                                                                                  aaTGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCCCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:1209955/239‑1 (MQ=255)

                                                                                                                                                                                                    ttgccTTAACCC‑GAAACGGGCCCAATCCCGTTTTTTGAGGTTATTTCCCACCAGCCAAAGGGT‑AA‑TTTAGCGAGACAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCCCGGTGAGCGTTTTGCTTCAGGAAATGATCCCGGCTGGAAAAACTGCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGcc                                      <  2:135826/236‑1 (MQ=255)

                                                                                                                                                                                                       ccTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:1067014/234‑1 (MQ=255)

                                                                                                                                                                                                          tAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTctg                                <  1:1109974/239‑1 (MQ=255)

                                                                                                                                                                                                                  cAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTGTCAGTAACGA‑CGCCCGCCGATCAATACCAATTAAATGACCGGGGGACTAAAAACCCCCTTctctttgtg                          >  2:1067014/1‑231 (MQ=255)

                                                                                                                                                                                                                   aaCTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAAAGG‑CGCCAGCCGGTCAATACCAATTAAATGACCGGGGGCCTAAGAACCCCaattcgcggtttgc                        >  2:1111113/1‑226 (MQ=255)

                                                                                                                                                                                                                    tttGGCCCCCTCCTCGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGTGTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  2:1113466/237‑1 (MQ=255)

                                                                                                                                                                                                                    aCTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:57586/239‑1 (MQ=255)

                                                                                                                                                                                                                    aCTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:1060750/239‑1 (MQ=255)

                                                                                                                                                                                                                    aCTGGCCGCATCCCAGTTTTTTAGGTGATTTTCCACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:1056974/239‑1 (MQ=255)

                                                                                                                                                                                                                    aCTGGCCGAATCCCAGTTTTTTAGGTGATTTTCGACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGAAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:1094443/239‑1 (MQ=255)

                                                                                                                                                                                                                      tGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACt                     <  1:246305/239‑1 (MQ=255)

                                                                                                                                                                                                                                         tAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCCGTAACGA‑CGCCAGCCGAGCAATACCCATTAACTGACCGGGCGGCCAAGGACCCCATCCTGCCGATTGCCCTGACTTTTTTGGGGTTCttt  >  2:953176/1‑239 (MQ=255)

TGGTGAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCA‑GCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGG‑AA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTT  >  NC_000913/4638642‑4639111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`