Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 855,390 T→G 13.6% T119P (ACC→CCC)  ldtB ← L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913855,3900TG13.6% 331.0 / 3.9 130T119P (ACC→CCC) ldtBL,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base T (36/68);  new base G (23/2);  total (60/70)
Fisher's exact test for biased strand distribution p-value = 1.38e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTACCTTCAGGAATGGTGATCACCTGAT  >  NC_000913/855157‑855626
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
ggTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGGTAGGGGGATCTTTGCCTAACTGACCAATGCCGGTCGGGAGAACGAAAAAGGGGGTgg                                                                                                                                                                                                                                          >  2:370484/1‑239 (MQ=255)
  gtccAGGGAGAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGt                                                                                                                                                                                                                                         <  1:1090583/236‑1 (MQ=255)
           tcgCATCCAGGCGACCGATATAGAGGGCATACAGCCCCAGCGGGTTATCCGGCCCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGa                                                                                                                                                                                                                              <  2:1371677/237‑1 (MQ=255)
            aGCATACAGGCGCCCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGAt                                                                                                                                                                                                                             <  1:738772/239‑1 (MQ=255)
            aGCATACAGGCGACCGATGTAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAAATGACCAATGCCGATCGGCGGCACGATAACGGTGTTGGTCCCTTTCGGAt                                                                                                                                                                                                                             >  1:358065/1‑239 (MQ=255)
            aGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCCTTTTGGCGGTCGGCGTCCAGGCCGGGCCTGCTTTTTTTAGCCCAACTTTGGGGGGCCAGTTGATAGGCGAATCTTTTCCTAACTGACCAATGCCCATCGGCGGGACGATACAGGGTTAGGGCCCTTTTGGaa                                                                                                                                                                                                                             >  2:217180/1‑238 (MQ=255)
              cATACAGGCGACCGATTAAGGGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGAt                                                                                                                                                                                                                             <  1:552052/237‑1 (MQ=255)
              cATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGGGGTCCCGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGTTCGGCTGCCCGAAAACGGGGTTGGGCCCTTTCgggaag                                                                                                                                                                                                                           >  2:1010900/1‑235 (MQ=255)
                   aGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGGGTTGGTCCCTTTCGGATGGtaata                                                                                                                                                                                                                      >  1:50683/1‑239 (MQ=255)
                        aCCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGTACGGCCGCACGATCACGGGGTTGGTCCCTTTCGCAATGTaaaaaaaaa                                                                                                                                                                                                                 >  2:1091794/1‑236 (MQ=255)
                        aCCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGGCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGAAAGGGGGATCTTTTCCTAACTTGCCAATGCCCTACGGGAGACCGATAACGGTGTTGGGCCCTTTTGGGTAGGAAAAAAAAc                                                                                                                                                                                                                 >  2:1123497/1‑239 (MQ=255)
                        aCCGAAATAGAGTGCATACAGCCCCATCGGGTTACCCGGGCCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGa                                                                                                                                                                                                                  <  1:972263/238‑1 (MQ=255)
                          ggataatgggTGCATACGACCCCATCGGGTTATCCGGCCCTGCCGGAACGACAGCCGGAGGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACg                                                                                                                                                                                                                <  1:641796/229‑1 (MQ=255)
                          cGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTAAGCTCAACTTTGGTGGTCCCGTTGGTAGGCGTATCTTTGCCTAACTGACCAATGCCGTACGGCCGCCCCAAAAAGGGGTTTGGCCCTTTTggtaagaaaaaaaaaacc                                                                                                                                                                                                               >  2:518234/1‑223 (MQ=255)
                              atgggtggcatcacgCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCAtct                                                                                                                                                                                                           <  1:1273672/225‑1 (MQ=255)
                              ataGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCAtct                                                                                                                                                                                                           <  1:972673/239‑1 (MQ=255)
                              aaggagGGCATGCCGCCCCATCGGGTTAGCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCAtct                                                                                                                                                                                                           <  1:422427/236‑1 (MQ=255)
                                  agTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGGGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGc                                                                                                                                                                                                       >  1:1102559/1‑239 (MQ=255)
                                  agTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGGGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCCATCGGCAGCACGGTAAAGGGGGTGGTCCCTTTTGGACAGTaaaaaaacagcaatccagc                                                                                                                                                                                                       >  2:1002961/1‑226 (MQ=255)
                                   gTGCATACAGCCCCATCGGGTTACCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAg                                                                                                                                                                                                        <  1:169857/237‑1 (MQ=255)
                                    tGCATCCAGCCCCATCGGGTTATCCGGACCTGCCGGAACGGCAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCa                                                                                                                                                                                                      <  1:11752/238‑1 (MQ=255)
                                    tGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGGGTTGGTCCCTTTCGGATAGTAATAAAACAGCATCTCGGc                                                                                                                                                                                                       >  1:124921/1‑237 (MQ=255)
                                    tGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGGGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCCGTTGGTAGGCGGATCTTTTGCTAACTGACCAATGCCCATCGGCACCACGGAAAAGGTGTTGGTCCCTTTTCGGAAATAAAAAAGACGCCtcccgcca                                                                                                                                                                                                      >  2:641796/1‑232 (MQ=255)
                                    tGCATACAGCCCCATCGGGTTACCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCa                                                                                                                                                                                                      <  1:971386/238‑1 (MQ=255)
                                     gCATACAGCCCCATCGGTTTATCCGAACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCGGCGTGCATTTTGGCGGTCGGCGTCCTGGTCGGGCCTGCTTTTTTATGCCCAACTTTAGGGGTCCAGTTCATGAGCGTATCTTTGCCTTCCTGTCCCATGCCGACCGCCATCCCGAAAACGGAGTTTGTGCCTTTCAGGCAGTACAAAACACGcctactagcact                                                                                                                                                                                                    >  2:754843/1‑228 (MQ=255)
                                      cATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGc                                                                                                                                                                                                       >  1:249909/1‑235 (MQ=255)
                                      aagtcAGGCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTTCTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACt                                                                                                                                                                                                    <  1:1069889/234‑1 (MQ=255)
                                       aTACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGGTAACGGGGTTGGTCCCTTTCGGATAGTAATAAAAAccaccctcaccaccgt                                                                                                                                                                                                  >  2:169857/1‑224 (MQ=255)
                                       aTACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGt                                                                                                                                                                                                  >  1:902138/1‑239 (MQ=255)
                                            gCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGa                                                                                                                                                                                                                              <  2:1100355/206‑1 (MQ=255)
                                            gCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGGGTTGGTCCCTTTCGGGTCGTAATAAAGACGCATCTCAGCACTGTTAATg                                                                                                                                                                                             >  1:331797/1‑239 (MQ=255)
                                             ccccATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGCTAACGGTGTTGGTCCCTTTCGGGTAGTAATAAAGACGCATCTCAGCACTGTTAATg                                                                                                                                                                                             >  1:239369/1‑238 (MQ=255)
                                             ccccATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGGCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGGATCTTTGCCTAACTGACCAATTCCGATCGGCAGCCCGATAACGGGGTTTGTCCCTTTCGGGTATGAATAAAGACGCCTCCCCACACGGTTAAAGa                                                                                                                                                                                            >  2:1225549/1‑239 (MQ=255)
                                               ccATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACg                                                                                                                                                                                          <  1:1010900/239‑1 (MQ=255)
                                                cATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGAt                                                                                                                                                                                                                             <  2:227775/203‑1 (MQ=255)
                                                 aTCGGGTTATCCGGGCCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGAt                                                                                                                                                                                        <  1:1262505/239‑1 (MQ=255)
                                                  tCGGGTTGTCAGCCCCGGCCGGAACCACAGCCGGAAGGGGTGCGCCCGACGCGGTGGACTCTTCGGGGACTTTGGCGGTCGGCCTGCAGGTCGGGCCTTCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATg                                                                                                                                                                                       <  2:779069/239‑1 (MQ=255)
                                                  tCGGGTTATCCGGCCCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTATTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATg                                                                                                                                                                                       <  1:217180/239‑1 (MQ=255)
                                                  tCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCCATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAAAAAAAACGCAACCCAACACCGGTAATGACGAt                                                                                                                                                                                        >  2:1000743/1‑238 (MQ=255)
                                                  tCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCCCGATAACGGTGTTGGGCCCTTTCGGAAAGGAATAAACACGCAACTAAGCACTGTTAATTACGAt                                                                                                                                                                                        >  1:759126/1‑238 (MQ=255)
                                                    gggTTTTCCGGGCCTTCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGcc                                                                                                                                                                                     <  2:529449/239‑1 (MQ=255)
                                                    gggTTATCCGGACCTGCCGGAACGGCAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGGGCATTTTGGCGGGCGGCGGCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGcc                                                                                                                                                                                     <  1:654385/239‑1 (MQ=255)
                                                       ttATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCCGGTCGGGCCTGCTTTTTTACGCTCAACCTTGGTGGTCCAGTTGATAGGCGTATCTTTTCCTAACTGACCCATGCCGATCGGCAGCCCGATAACGGGGTTGGTCCCCTTCGGAAGATTAAAAAGACGCATCTCacaccgtttaaaggagatggctca                                                                                                                                                                                  >  2:1148596/1‑216 (MQ=255)
                                                       ttATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCCCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGCTCGGCAGCACGATAACGGGGTTGGTCCCTTTCGGCTAGTAATAAAGACCCCTCTCacaccctttaaagacactgacctc                                                                                                                                                                                  >  2:1262505/1‑216 (MQ=255)
                                                         aTCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCCGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGGATCTTTGCCTAACTGACCAATGCCGATCGGCAGCCCGCAAACGGGGTTGGGCCCTTTCGGGAAGGAATAAAAAAGCACCCCCACCATGTTAAGGACCATGCCTTCAt                                                                                                                                                                                >  2:1266557/1‑239 (MQ=255)
                                                         aTCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGGTAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCAt                                                                                                                                                                                >  1:1116309/1‑239 (MQ=255)
                                                          tCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTCCGCTCAACTTTGTTGGGCCAGTTGAGAGGCGTATCTTTGCCTAACTGACCAATGCCGTTCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGAAAGTGATAAAGACGCATTTCAGCCCGTTTAAAGACGAAGCCGTccat                                                                                                                                                                               >  2:250895/1‑236 (MQ=255)
                                                            gcgcccTGCCGGCACGGCAGGCGGAAGCGGTTGCCCCGGCGCGCGGTTCTCGGCGTGCATTTTGGCGGTGGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTGGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGaa                                                                                                                                                                             <  2:5312/235‑1 (MQ=255)
                                                               aCCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATCAAGACGCATCTCCGCACTGTTAATGACGATGCCTTCATGAACgg                                                                                                                                                                          >  1:1222677/1‑239 (MQ=255)
                                                                    ccGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGCTAACGGTGTTGGTCCCTTTCCGATAGGAATAAAAACGCACCTCAGCACTGGTAATGACGCAGCCTTCATTAACGGTATcc                                                                                                                                                                     >  2:846889/1‑239 (MQ=255)
                                                                     cggggagacgccgggAAGCGGTTCGCCCGCAGGGCGGTTTTCTGCGTGCATTTTTGCGGTCGGCGTCCAGGTTGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCg                                                                                                                                                                    <  2:1204324/226‑1 (MQ=255)
                                                                      tgacccaccagcGGGAGGGGTTCCGCCGGAGCGTCGTGCTTTGGGTGCATTTGGGGCGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCg                                                                                                                                                                    <  2:27047/227‑1 (MQ=255)
                                                                       gaacaccAGCCGGAAGCGGTTCGCCCGCGGCGCGGTACTCTGCGGTCATTTTGGCGGTCGGCGTCCAGGGCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGc                                                                                                                                                                  <  2:50683/233‑1 (MQ=255)
                                                                                     aGCGGTTCGCCCGTAGCGCGGTACTTGGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAgctgct                                                                                                                                                     <  1:630741/238‑1 (MQ=255)
                                                                                          ttCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGa                                                                                                                                                <  1:698976/238‑1 (MQ=255)
                                                                                          ttCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGa                                                                                                                                                <  1:1278588/238‑1 (MQ=255)
                                                                                          ttCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGa                                                                                                                                                <  1:1123497/238‑1 (MQ=255)
                                                                                          ttCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGa                                                                                                                                                <  1:1091794/238‑1 (MQ=255)
                                                                                          ttCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGa                                                                                                                                                <  1:1000743/238‑1 (MQ=255)
                                                                                              cccGCCGGGCGGTACTCTGCGTGCATTTTGGGGGTGGGCGTCCCGGTCGGGCCTGCTTTTTTACGCTGAACTTGGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACGGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGtt                                                                                                                                           <  2:1222677/239‑1 (MQ=255)
                                                                                              cccGCCGCGCGGGTCTTTGCGTGTCTTTGGGCGGTCGGGTCACAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGtt                                                                                                                                           <  2:239369/239‑1 (MQ=255)
                                                                                              cccGCAGGGCGGTACTCGGGCGGTATTTTGGGGGTCGGGGTCCAGGTCGGGTCTGGTTTTTTACGCTCAACTTTGGTGGGCCAGTTGATAGGCGTATTTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGtt                                                                                                                                           <  2:395242/239‑1 (MQ=255)
                                                                                              cccGCAGCGCGGTACTCTGGGTGCATTTTGGCGGGCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGtt                                                                                                                                           <  1:846889/239‑1 (MQ=255)
                                                                                               cggCAGCGGGGGTCTCTGCGTGCATTTTGGCGGTCGGCGTTCAGGTCGGGCCTGCTTTTTTAACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGtt                                                                                                                                           <  2:847018/237‑1 (MQ=255)
                                                                                                   cggcggttttcttgggTGCTTTTGGGCGGGGGGCGCCCAGGCGGGGCCTTCTTTTTTCCGCTCAATTTTGGTGGTCCAGTTGATAGGCTTATCTTTGCCTAACTGCCCAAGGCCGATCGGCAGCCCGATACGGGTGTTGGTCCCTTTGGGTGAGTAATAAAGCCCCATCTCAGCACGTTTATTGCCGTTGCCTTCAGGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTcagta                                                                                                                                      <  2:1102559/224‑1 (MQ=255)
                                                                                                          tACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCagtacagtac                                                                                                                                <  1:1148596/238‑1 (MQ=255)
                                                                                                                gCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCttt                                                                                                                         <  1:1002961/239‑1 (MQ=255)
                                                                                                                    gCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGc                                                                                                                     >  1:136590/1‑239 (MQ=255)
                                                                                                                    gCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAAACGCATCTCAGCCCGGTTAATGACGATGCCTTCATTAACGGGATCCGGCAGGATCAGCTGCTGGGGGAATTTCAGTACAGAACCGGCTTTCggg                                                                                                                     >  2:915570/1‑238 (MQ=255)
                                                                                                                     cATTTGGGCGGTCGGCGTCCAGGTCCGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGc                                                                                                                     <  1:5556/238‑1 (MQ=255)
                                                                                                                        tttGGCGGTCCGGTGCCAGGGTGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTGGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAgg                                                                                                                  <  2:249909/238‑1 (MQ=255)
                                                                                                                               gTCGGCTTCCAGGTCGGGCCTGCTTTTTTACGCTCAACGTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTAt                                                                                                           <  1:1167889/238‑1 (MQ=255)
                                                                                                                               gTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTCCCGCGGAATGTTCAGGACAGTACCCCCTTTCGCCAGGAAggaaa                                                                                                           >  2:564461/1‑235 (MQ=255)
                                                                                                                                tCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGGATCCGGCAGGATCAGCTGCTGCGGAATGTTCCGTACAGTACCGcccctgtcccttatacccat                                                                                                          >  1:246920/1‑220 (MQ=255)
                                                                                                                                                    tgtttttttACGCTTAATTTTTGTGGTTCAGTTGAAGGGCGTTTCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCca                                                                                      <  2:136590/236‑1 (MQ=255)
                                                                                                                                                    tGCTTTTTTACGCTCAACTTTTGGGGTCCAGTTGATAGGCGTATCTTTTCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCca                                                                                      <  1:1011612/239‑1 (MQ=255)
                                                                                                                                                    tGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCca                                                                                      <  1:1225549/239‑1 (MQ=255)
                                                                                                                                                      cTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAg                                                                                                                                                          >  2:151336/1‑169 (MQ=255)
                                                                                                                                                               cGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCGTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGa                                                                            <  1:130664/239‑1 (MQ=255)
                                                                                                                                                                 cTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAgct                                                                                                                                                        >  1:363383/1‑161 (MQ=255)
                                                                                                                                                                 cTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAgct                                                                                                                                                        <  2:363383/161‑1 (MQ=255)
                                                                                                                                                                              gtCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTCCAGTACCGCCttt                                                                                                                         >  2:937895/1‑179 (MQ=255)
                                                                                                                                                                              gtCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCttt                                                                                                                         <  1:937895/179‑1 (MQ=255)
                                                                                                                                                                              gtCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGATGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGAATCCACACCCGGGTTCGCTTCCACCATATTGGAAAGCCCCACCTGGt                                                              >  1:554713/1‑238 (MQ=255)
                                                                                                                                                                                         aGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGGACTCGGCGGCaa                                                  >  1:236643/1‑239 (MQ=255)
                                                                                                                                                                                             gTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCg                                                                                                                                                  >  1:87309/1‑139 (MQ=255)
                                                                                                                                                                                             gTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCg                                                                                                                                                  <  2:87309/139‑1 (MQ=255)
                                                                                                                                                                                              tATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCg                                                                                                                                                                    <  1:1332792/120‑1 (MQ=255)
                                                                                                                                                                                              tATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGa                                                                                                                                                >  1:174442/1‑140 (MQ=255)
                                                                                                                                                                                                   ttGCCTAACTGACCAATGCCTATCGGCAGCACGATAACGGTTTTGGTCCCGTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAg                                        <  1:982853/239‑1 (MQ=255)
                                                                                                                                                                                                     gCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGAATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCg                                      >  1:918146/1‑239 (MQ=255)
                                                                                                                                                                                                       cttaCTGCCCAAGGGCGATCGGGAGAACGAAAACGGGTTTTGTTCTTTTCGGATAGTAATAAAGACGCATCCAGGCCTTGGTAATGGAGAGTCCTTCAAGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGc                                    <  2:62131/236‑1 (MQ=255)
                                                                                                                                                                                                                cAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGAATCCACACCCGGGGTAGCTTCCATCAAATTGGAAAGCCCCATCCTGAAACCGGCGGCAAAATACTCCacaggcgggggtgt                            >  2:597527/1‑225 (MQ=255)
                                                                                                                                                                                                                  aTGCCTATCGGTCACACGATAACGGTTTTTGTCCCCTTCGGGTAGTAATAAAGACGCATCTCCTCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTAcc                         <  2:1116309/239‑1 (MQ=255)
                                                                                                                                                                                                                     cctgtgggcagaagtgtAACGTTGTTTGGTCCTTTCGGATAGTAATAAAGACGCATCGCAGAACTGTTAAGGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTACCTTc                      <  2:948790/223‑1 (MQ=255)
                                                                                                                                                                                                                        aTCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCAGGCGGCTGATGGTTAACTTCAgg                   >  1:948790/1‑239 (MQ=255)
                                                                                                                                                                                                                          cGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTACCTTCAGGaa                 <  1:518234/239‑1 (MQ=255)
                                                                                                                                                                                                                            ggcgcccgttAACGGGTTTGGTCCCCTTCGTGTTGTAATAAAGATCCATCTCAGGACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTACCTTCAGGAATg               <  2:1268720/230‑1 (MQ=255)
                                                                                                                                                                                                                                     tAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCAGGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCTGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTACCTTCAGGAATGGTGATCAcc      <  1:705916/239‑1 (MQ=255)
                                                                                                                                                                                                                                          gtgtTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCACCGGCTGAGTGTTACCTTCAGGAATGGCGATCACCCGAt  >  1:1330509/1‑238 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
GGTGCCATGGATAGCATACAGGCGACCGATATAGAGTGCATACAGCCCCATCGGGTTATCCGGACCTGCCGGAACGACAGCCGGAAGCGGTTCGCCCGCAGCGCGGTACTCTGCGTGCATTTTGGCGGTCGGCGTCCAGGTCGGGCCTGCTTTTTTACGCTCAACTTTGGTGGTCCAGTTGATAGGCGTATCTTTGCCTAACTGACCAATGCCGATCGGCAGCACGATAACGGTGTTGGTCCCTTTCGGATAGTAATAAAGACGCATCTCAGCACTGTTAATGACGATGCCTTCATGAACGGTATCCGGCAGGATCAGCTGCTGCGGAATGTTCAGTACAGTACCGCCTTTCGGCAGGAAGGTATCCACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATACTCCAGCGGCTGAGTGTTACCTTCAGGAATGGTGATCACCTGAT  >  NC_000913/855157‑855626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: