Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,123,412	T→A	13.4%	I215F (ATT→TTT)	grxB ←	glutaredoxin 2 (Grx2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,412	0	T	A	13.4%	155.0 / 2.4	71	I215F (ATT→TTT)	grxB	glutaredoxin 2 (Grx2)
Reads supporting (aligned to +/- strand):  ref base T (11/48);  new base A (8/2);  total (21/50)
Fisher's exact test for biased strand distribution p-value = 2.86e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

TTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAG  >  NC_000913/1123176‑1123628                                                                                                                                                                                                                                                |                                                                                                                                                                                                                            ttAATTTTTTTTGCGGTGTTGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCg                                                                                                                                                                                                                           <  2:1319932/239‑1 (MQ=255)     tttCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGACAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCAGCGGCGGGA‑CC‑GGA‑TTTAATCG‑ccct                                                                                                                                                                                                                      >  1:1193359/1‑237 (MQ=255)          tGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGCCGGCAACCCCCGAATACAGGCTGCCCCGGCGGGG‑CC‑GGA‑TTAAAACG‑CCCTtgatg                                                                                                                                                                                                                 >  1:1255141/1‑239 (MQ=255)           tttgggTGTTGGGTTATTGGGTGTGTTGATATCAGCCAACGGGCCGGCCATTGACGATCAATGACGCGGCAAATAAAATCTTGTTCATGGTAGTCCTCGACATGAAATCTGAGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATtgatga                                                                                                                                                                                                                <  2:394907/235‑1 (MQ=255)                   aTGGTGTATTGGGTGAGTTGATTAAAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACaaa                                                                                                                                                                                                         <  1:895402/238‑1 (MQ=255)                    tGGTGTATTGGGTGATTTGATTACAGCCAACGGGCAGGGCGCTGACGATCAATGCAGCGGCAAATAAAAACTTGGTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAAt                                                                                                                                                                                                        <  1:520428/238‑1 (MQ=255)                           ttGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CC‑GGA‑TTTAAAAC‑CCCCAGATGATAACAAATTGAttttg                                                                                                                                                                                                >  1:331921/1‑237 (MQ=255)                           ttGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGG‑CA‑GGG‑TTAAAATG‑CCCTCTATGATAACAAATTGAttttg                                                                                                                                                                                                >  1:1171523/1‑237 (MQ=255)                           ttGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCATCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGTCGGCAACCCGCGAATAAAGGCTGCCGTGGCGGGG‑CG‑GGG‑TTAAAATG‑CCATTGTTGATAACAAAttacatttt                                                                                                                                                                                                >  1:1081610/1‑232 (MQ=255)                                tGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCCGCCGCGGGGGGG‑CA‑CGAGTAAAACCC‑CCATTGATGAAAAAAAAAT‑ATTtgtgggtg                                                                                                                                                                                           >  2:707880/1‑235 (MQ=255)                                   gTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTCCCCCGGCGGGG‑CA‑GGA‑TGAAAACA‑CCCATGATGAAAACaaaaaaatttgggttgtttt                                                                                                                                                                                        >  2:1275418/1‑222 (MQ=255)                                     ttaTTACACCCAACGGGCAGGCCACTGACGATCAATGCAGCGGAAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCg                                                                                                                                                                                      <  2:1274404/237‑1 (MQ=255)                                     tGATTAAAGCCAACGAGCAGGCCACTGACGATCAATGCCGCGGAAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCGGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCg                                                                                                                                                                                      <  2:411703/239‑1 (MQ=255)                                        tttcagcacaccggaaGGGCATTGGCGATCAATGCAGCGGAAAATAAAAACTTGTTCATGGTGCGCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCa                                                                                                                                                                                   <  2:649823/224‑1 (MQ=255)                                        ttACAGCCAACGAACAGGCCAAGGACGATCAATGCAGCGGCAAATAAAAACTTGTTCAGGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCa                                                                                                                                                                                   <  1:148063/239‑1 (MQ=255)                                         tACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACCGGGTGCCGCGGCGGGG‑GA‑GGG‑TGAAAAAGCCCATTGGTGAAAAAAAAtaatttttgttgtttttgccaa                                                                                                                                                                                   >  2:1168044/1‑218 (MQ=255)                                              ccAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAAAAG‑CCATTGATGATAACAAATTGATTTTTGTCTGTTTTCCCAcatta                                                                                                                                                                              >  1:567628/1‑238 (MQ=255)                                                agaggccgggcccatGACGATAAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACAGAAAATCGGCGTCAATATCCTGACACAACGCAGCATGTGTCCCCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATcg                                                                                                                                                                           <  2:1343252/225‑1 (MQ=255)                                                aaCGGGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGCCATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATcg                                                                                                                                                                           <  1:159106/239‑1 (MQ=255)                                                  cGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATcgcg                                                                                                                                                                         <  1:335458/239‑1 (MQ=255)                                                        ggCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCCCCAGCAGAAAAAACTGAAAACGGCGGCAACCCCCGCATACAGGCTGCCGCGGCGGGTCCA‑GAA‑TAAAAACC‑CCATT‑ATGATTACAAAATGTTTTTTTTCTTCTTCGCCCCATTTTTGCGGGaaa                                                                                                                                                                    >  2:685447/1‑237 (MQ=255)                                                              tGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATAAAGGCTGCCGCGGCGGGG‑CAGGGT‑TTAAACCC‑CCATTTTGGAAAAAAAATT‑CTTTTTGTTTTTTTTGCCataattttccgggaataaaaaa                                                                                                                                                              >  2:516468/1‑219 (MQ=255)                                                                   aTCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGG‑TTAAATCG‑CCATTTAGGTTAAAAAATTGATTTGTGTCTGGTTTGCCCAATTATTCCGGTAATCAGCAAagcg                                                                                                                                                          >  1:461814/1‑237 (MQ=255)                                                                    tCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTGGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGc                                                                                                                                                        <  1:345761/238‑1 (MQ=255)                                                                       aTGCAGCGGCAAATAAAAACTTGTTCATGGCAATCCTCGGCATGAAATCTGCGTCAACATCCTGACACAACGCCGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTgg                                                                                                                                                    <  1:87388/239‑1 (MQ=255)                                                                         tcAGCGGCAAAAAAAAACTTGTTCCAGGTAGGCCTCGACATGAAATCGGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACCCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGcc                                                                                                                                                  <  2:1255141/238‑1 (MQ=255)                                                                                         cgtttttaatggtgtgccTCGAAATGAAATTTGCGTCAATATCCTGACACAACGGAGAATGTGGCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTAc                                                                                                                                 <  2:567628/223‑1 (MQ=255)                                                                                         aCTTGTTCTTGGTATTCTCCGACATGAAATCTTCCTCAAATTCCCGACACAACGCAGCATGGGTCACCAGCCATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATCAAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTAc                                                                                                                                 <  2:67523/239‑1 (MQ=255)                                                                                              ttCATGGTAGTCCTCGACATGAAATCTTCTTCAAATTCCTGACAAAACGCAGCATGTGTCCCCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCg                                                                                                                            <  2:461814/239‑1 (MQ=255)                                                                                              ttCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCg                                                                                                                            <  1:1275418/239‑1 (MQ=255)                                                                                              ttCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACAAAACGCAGCATGGGGCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATcg                                                                                                                                                                           <  2:440274/193‑1 (MQ=255)                                                                                                        tCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTTAATCG‑CCATTTATGATAACAAATTGATTTTTTTCTTGTTCTCCATATTATCGCGGTAATCAGCCAACCGGCTTTGCCCG‑TTAATTCCGGCCACCcgcgcccgctttc                                                                                                                   >  1:1065573/1‑229 (MQ=255)                                                                                                        agacagACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACg                                                                                                                  <  1:118634/234‑1 (MQ=255)                                                                                                         ccTAGAAATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACg                                                                                                                  <  1:940368/238‑1 (MQ=255)                                                                                                          ccggACATGAAATCTGCGTCAATATCCTGCAACAACGCAGCATGGGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCa                                                                                                                <  1:1168044/236‑1 (MQ=255)                                                                                                          cTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCa                                                                                                                <  1:516468/239‑1 (MQ=255)                                                                                                               cATGAAATGTGGGTCAATACCCTGAAAAAACGAAGCATGTGTCACCAGCGATAAACCCGCCAGCAGAAAAAACGGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAg                                                                                                            <  1:685447/238‑1 (MQ=255)                                                                                                                          cGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCg                                                                                                                                                                                                                           <  2:368250/118‑1 (MQ=255)                                                                                                                          cGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCg                                                                                                                                                                                                                           >  1:368250/1‑118 (MQ=255)                                                                                                                                       acacAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGGAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCCGATTACGCAGTAACGGGGATAGCC‑GAATATCCTCTTc                                                                                   >  1:316441/1‑238 (MQ=255)                                                                                                                                             cGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTTGCCATATTATCCCGGTAATCAGCAACCCGGCTTGGCCCA‑TTAATTCCGGCTACCCGCGTCAGGTTACGGCGCAACGGGGAAAGCT‑GAATATCATCTTCCCAAAGt                                                                            >  1:1092644/1‑239 (MQ=255)                                                                                                                                             cGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCA‑CCCTTGATGATAACAAATTGATTTGTGTCTGTTTCGCCCTATTATCGCGGGAATCAGCAACCCGGCTTGGGCAG‑TTAATTTCGGATACCAGCGTGAAATTACGCAGGAGCGGGGAAAGCT‑GAATATCATATTCCGcaaaa                                                                            >  1:152029/1‑237 (MQ=255)                                                                                                                                             cGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGG‑CA‑GGA‑TTAAATCG‑CCATTTATGATAACAAATTTATTTGTGTCTGTTTTGCCATATTATCCCGGTAATCAGCAAAACGGCCTTGCCAGTTTAATTCC‑GCTACCACCGTCCAATTTACCAGTAGCGGGGAAAACTGGAATAT‑ATCTTCCCaaaaa                                                                            >  1:91282/1‑237 (MQ=255)                                                                                                                                                agcaTGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTc                                                                          <  1:769775/238‑1 (MQ=255)                                                                                                                                                  caTGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGc                                                                        <  1:122916/238‑1 (MQ=255)                                                                                                                                                          aCCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGG‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAAATGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGGTTAAGCAGTAGCGGGAAAAGCT‑GAAAATCATCTTCCGAAAATTGCCCATTCCCgg                                                               >  1:1226679/1‑239 (MQ=255)                                                                                                                                                              gCGATAAACCCGCCAACAGAAAAAACTGAAAACGGCGGCAACCCTTGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGtt                                                           <  1:1007384/239‑1 (MQ=255)                                                                                                                                                                         ggcagcagAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGGGGCGGGT‑CA‑GGA‑TTAAAACG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGa                                                <  1:124050/237‑1 (MQ=255)                                                                                                                                                                         cccAGCAGAAAAAACTGAAAACGGGGGCAACCCGCGAATACAGGCTGGCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGa                                                <  2:1171523/238‑1 (MQ=255)                                                                                                                                                                         cccAGCAAAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGa                                                <  1:225412/238‑1 (MQ=255)                                                                                                                                                                              cagAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAAc                                                                                                                                                                                                            <  1:319117/80‑1 (MQ=255)                                                                                                                                                                               agAAAAAACTGAAAACGGCGGCACCCCGGGAAATCAGGCTGCCGGGGGGGGT‑CC‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGGGTCTTTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGt                                           <  2:415585/238‑1 (MQ=255)                                                                                                                                                                               aaaaaaaaCTGAAAACGGCGGCAACCCGGGAATACGGGCTGCCGCGGCGGGG‑CA‑GGA‑TTAAATCG‑CCATTGATTATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGt                                           <  2:1092644/236‑1 (MQ=255)                                                                                                                                                                                      aCTTAAAACGGCGGCAACCCGGGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAg                                   <  1:1203779/239‑1 (MQ=255)                                                                                                                                                                                      aCTGAAAACGGCGGCAACCCGGGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAg                                   <  1:707880/239‑1 (MQ=255)                                                                                                                                                                                       cTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAgc                                  <  1:945020/239‑1 (MQ=255)                                                                                                                                                                                        tGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTTAATCG‑CCATTGTTGATAAAAAATTGAttt                                                                                                                                                                                                  >  2:319117/1‑80 (MQ=255)                                                                                                                                                                                                 ccccacccccccattACCGGTTGGCGGGGGGGGT‑CA‑GGA‑TTAAATTG‑CCATTGATGATAACAAATTGTTTTGTGGTTGTTTCCGCAAATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTTCCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAat                        <  2:1158429/225‑1 (MQ=255)                                                                                                                                                                                                     aaCCCGCGAAATAAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAatcat                     <  2:152029/238‑1 (MQ=255)                                                                                                                                                                                                         ccctaatacgttgggtccgtgcggGT‑‑A‑GGA‑TTAAATCT‑CCATTGAGGATAACAAATTGATTTGTGTTTGTTGCGCTTGATTATCGCGGTAATCCGAGATGGTGCTTGGTCAG‑TTAAGTTCGGCTACCAACGTCAGATTTCGCAGTAGCGGGAATAGCT‑TAATATCATCTTCCGAAAGTTAGCTATTCACGGCGTTCGGTTTGACGATCAGTGTGTCCAGCGCACGTAAATGATCGCTGa               <  2:710080/219‑1 (MQ=255)                                                                                                                                                                                                          gtcaatacgggtttgcgcgGCGGTT‑CA‑GGA‑TTAAATCG‑CCATTGATGAAAACAAATTGTTTTGTGTGTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGa               <  2:577481/224‑1 (MQ=255)                                                                                                                                                                                                          gggAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGa               <  1:143073/237‑1 (MQ=255)                                                                                                                                                                                                          cgaaaTAAAGGCGGCCGGGGGGGGT‑CA‑GGA‑TTAAATCC‑GCATTGATTCAAACAAATTGGTTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGa               <  2:1193359/236‑1 (MQ=255)                                                                                                                                                                                                                     cttccGCGGGGGGT‑CA‑GTA‑TTTAATTG‑CCATTGGTTATAACAAATTGCGTTGTTTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAAt     <  2:470929/235‑1 (MQ=255)                                                                                                                                                                                                                     cTGCCGGGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGGGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAAt     <  1:584205/238‑1 (MQ=255)                                                                                                                                                                                                                     cTGCCCGGGCGGGT‑CA‑GGA‑TTAAAACG‑CCATTGATGATAACAAATTGATGTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAAt     <  1:1246041/238‑1 (MQ=255)                                                                                                                                                                                                                       ggcgcgGCGGGT‑CA‑GGA‑ATAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCa   <  1:1335326/236‑1 (MQ=255)                                                                                                                                                                                                                       gCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCa   <  1:474208/238‑1 (MQ=255)                                                                                                                                                                                                                        ccGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAg  <  1:659353/238‑1 (MQ=255)                                                                                                                                                                                                                                               aTCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGt                                                                            <  2:366693/144‑1 (MQ=255)                                                                                                                                                                                                                                               aTCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTAGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGt                                                                            >  1:366693/1‑144 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                            TTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGT‑CA‑GGA‑TTAAATCG‑CCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAG‑TTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCT‑GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAG  >  NC_000913/1123176‑1123628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑