Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,251,973	T→C	14.6%	L303P (CTC→CCC)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,251,973	0	T	C	14.6%	223.7 / 4.7	89	L303P (CTC→CCC)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base T (54/19);  new base C (3/11);  total (57/32)
Fisher's exact test for biased strand distribution p-value = 3.22e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCG  >  NC_000913/1251741‑1252206                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            cAATTTATAAAGTCCCAGGGGGCGGGGGTTTGGCCCCTTTGCCGC‑AGGGCAAGTAGGGGGAGGGAAAACCACAGAACTTTAAATTTTC‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATc                                                                                                                                                                                                                                        <  1:1261042/239‑1 (MQ=255)  aaTTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCAt                                                                                                                                                                                                                                         >  1:1020593/1‑236 (MQ=255)           aTGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTTTTTCCCCATCCTGGGGaaaaa                                                                                                                                                                                                                             >  2:566428/1‑236 (MQ=255)                cAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCCCCATCCTGTGGaccgaatgc                                                                                                                                                                                                                         >  1:1061158/1‑230 (MQ=255)                  ggcggcGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGc                                                                                                                                                                                                                     >  1:1325656/1‑239 (MQ=255)                               cgtgctcctgggccgggcGGAAAGG‑CAGGGACGGCCAATCAGTGAACTTTAAACTTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGAcc                                                                                                                                                                                                          <  1:1322961/221‑1 (MQ=255)                                       ttGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAAttt                                                                                                                                                                                                 >  1:1069069/1‑238 (MQ=255)                                        tGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAAATGC‑GGCCGTTGATGACCAGTCAATTAg                                                                                                                                                                                                >  2:58740/1‑239 (MQ=255)                                                             ggcgggaaatccaagAACTTTTAACTTCA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCtt                                                                                                                                                                             <  1:166030/224‑1 (MQ=255)                                                                 ggCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAAAAGATGC‑GGCAGGTGTTGACCAATCAATTAggctgtctcttataaaaatccgacg                                                                                                                                                                        >  2:48639/1‑216 (MQ=255)                                                                 gagcaaccacacaactttAACCTTAGCCCGCCGTAATGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAGGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCAGCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑Gc                                                                                                                                                                         <  1:37984/222‑1 (MQ=255)                                                                    aacccgtGAACTTTTAACTTTC‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCata                                                                                                                                                                    <  1:566428/233‑1 (MQ=255)                                                                     acccactacatcttccccttacccccccGTTTTGCAACAAGCAATAAAACAGGCCCATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCata                                                                                                                                                                    <  2:129289/217‑1 (MQ=255)                                                                            aaCTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGGAAAGTCAGCCATACCTTC‑GCTCATATGCCACAg                                                                                                                                                            >  1:766706/1‑239 (MQ=255)                                                                            aaCTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATAc                                                                                                                                                                                >  1:500856/1‑220 (MQ=255)                                                                                 ttAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACg                                                                                                                                                        >  1:382257/1‑238 (MQ=255)                                                                                 ttAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAATAAACCAAACCTTC‑GCCCAAAAGCCAAAGGagg                                                                                                                                                        >  2:536964/1‑236 (MQ=255)                                                                                   aaCGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGTTGACCAGCCAATTAGGAAAATTCAGCCAAACCTTcccccaatttcccaagagcgaac                                                                                                                                                      >  2:1302335/1‑217 (MQ=255)                                                                                   aaCGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCTTATGCACCAGGACGAt                                                                                                                                                      >  1:175526/1‑238 (MQ=255)                                                                                        tA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGCAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCaa                                                                                                                                                >  1:414858/1‑239 (MQ=255)                                                                                         a‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATGATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGAGCC‑GGCAGTTGTTGACCAGTCAATTAGGAAAAGTAAGCCATACCTTC‑CCCCATATGCACCAGGAAAAACCGCaa                                                                                                                                                >  2:37324/1‑238 (MQ=255)                                                                                         a‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGACCAACAGC‑GGCAGGTGACGACACGTCAATTGGGAAAGGTCAGCCTGAACTTC‑GCCCaaagtcaaaagagcaatcgcaaa                                                                                                                                                >  2:1135985/1‑216 (MQ=255)                                                                                         a‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCaa                                                                                                                                                >  1:32458/1‑238 (MQ=255)                                                                                         a‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCaa                                                                                                                                                >  1:770110/1‑238 (MQ=255)                                                                                         a‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTAATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GA‑CGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGGGGAACAGATGC‑GGCAGTGGCAGACCAGTCACTTCGGCAAAGACAGCCAAACCTTC‑GCTCATTTGCCAAAGGCCGAACCGCaa                                                                                                                                                >  2:87787/1‑238 (MQ=255)                                                                                                cgTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGGAAACccgc                                                                                                                                          >  1:264375/1‑238 (MQ=255)                                                                                                      ggaacaaGCAAAAAAACCGGCAAATCCGGTCAAACACGTAAAACCACCCTGTAAAAGCCAG‑CACCAGTTTATCGATCGGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCCCAATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGa                                                                                                                                   <  1:762216/237‑1 (MQ=255)                                                                                                      gCAAAAAGAAATAAAACAGGCACACCCGCTAAAACACGAAAAACCAACCTGTAAAAGCAAG‑CACCAGTTAATGGAGGGGTTG‑ATAACCTTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAATTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGa                                                                                                                                   <  2:1020593/239‑1 (MQ=255)                                                                                                          caaGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCCCTTGATTCa                                                                                                                               >  1:1162494/1‑239 (MQ=255)                                                                                                            aGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑TGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCAtt                                                                                                                             >  1:615370/1‑239 (MQ=255)                                                                                                            aGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTTTGGAAAAGATGC‑GGCAGTTGATACCCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTAATATGCCCAAGAACGATCCGCAAACccacccccggattactt                                                                                                                             >  2:1213822/1‑227 (MQ=255)                                                                                                            aGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATCC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGACAGCCAACCCTTG‑GTGCATATGCCCAAGGCCGATCCGACAACCCGCCGATTGTTTCttt                                                                                                                             >  1:1318887/1‑239 (MQ=255)                                                                                                             gCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGGAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCtttt                                                                                                                            >  1:807561/1‑239 (MQ=255)                                                                                                                 tAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTG‑GCTCAAATGCCCCATGACAGACCCCAAACCCCCCCCTGGAATCATTTTGGt                                                                                                                        >  2:1051268/1‑239 (MQ=255)                                                                                                                        ggCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAg                                                                                                                  >  1:680893/1‑238 (MQ=255)                                                                                                                        ggCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATCC‑GGCCGTTGATGACCAGTCCATTAGGACAAGTCAGCCCTACCTTC‑GCCCATATGCCACAGAGCGATGCCCAAACCCCCCCCTTGATTCATTTTgaccgacggg                                                                                                                 >  2:715199/1‑230 (MQ=255)                                                                                                                         gCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggc                                                                                                                >  1:1127884/1‑239 (MQ=255)                                                                                                                             aTCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCAAATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTctgcctcttata                                                                                                            >  1:78294/1‑228 (MQ=255)                                                                                                                                cgttCAAACCCGTAGAAGCAACTTTTGAAAGCCAA‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGAAAATAGAAAC‑GC‑AGGGA‑ACCAGTTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGAGGC‑GGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                                                         <  1:1296017/236‑1 (MQ=255)                                                                                                                                ccctacacaccttAGAAGCACCCTTTAAAAGCCCG‑CACAAGTTTATTGGGGCGGTA‑ATAACCCTAAAACGAAAAAAATAAAC‑GC‑AGGGA‑ACCACGTTTA‑TTTTGTTGCTCAATCCTGGGGAACAGATGC‑GGAAGGTGATGACCAGTCAATTAGAAAAAGTTATCCATACCTTC‑GATGATAGCCCGCAGGACGAGCCGCAAGCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                                                         <  2:1068486/227‑1 (MQ=255)                                                                                                                                     caccgcttaaaaccaccttttAAAAGCCAG‑CACGGTTTTATGGTGGCTGTA‑ATAA‑CCTTCAAACTAAAAAGGAAACGGG‑AGGGA‑AGCTGATTAA‑TTTTGTTGCGCCAGCCTGTGAAAAAGATGC‑GGCGGTTGGTGCCCAGTCAATTAGTAAAAGTCCGTCATCCCTTC‑GCTAATTTGCCACAGGACGAACCCCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                                                                    <  2:1252899/221‑1 (MQ=255)                                                                                                                                     aaaCCCGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTT‑ATACCCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGTTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                                                                    <  1:916654/239‑1 (MQ=255)                                                                                                                                     aaaCACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATACCCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                                                                    <  1:536964/239‑1 (MQ=255)                                                                                                                                     aaaCACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAAACCGCCGCTTGATTCATTTTGGCCGCCAGGCGgcgcgcggaa                                                                                                     >  2:551658/1‑234 (MQ=255)                                                                                                                                       acacGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGCTGC‑GGCAGCTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTG‑CCCCATATGCCACAGGCCGATCCGCAACCCCCCCCCTTGATTCTTTTTTGTCCCCAGGCTGCCCGCAgagcct                                                                                                  >  2:82456/1‑234 (MQ=255)                                                                                                                                           gTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCa                                                                                                                                                                                                         >  2:1306127/1‑133 (MQ=255)                                                                                                                                           gTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCa                                                                                                                                                                                                         <  1:1306127/133‑1 (MQ=255)                                                                                                                                                   caCCTTTTAAAACCAGCCCCCAGTTTATTGATGCGGTG‑AAACCCCATAACCAGATAAAAGAAAC‑GC‑AGGGA‑ACCCGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATAAGGAAAAGGCAGCAATTCCTCC‑GCTCATGGGCCACAGGCCGATCCGAAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                                                       <  2:32458/238‑1 (MQ=255)                                                                                                                                                    cccTTTAAAAGCCAG‑CACCAGTTTATGGATGCGGTG‑ATAACCCTTAACCCGATAATAGAAAC‑GC‑AGGGA‑ACCAGTTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                                                                     <  1:58740/238‑1 (MQ=255)                                                                                                                                                    aCCTTTAAAACCCAG‑CACCAGTTTATTGATGCGTGT‑AAAACCCTTAAACCAATAATAGAACC‑GC‑GGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATCCCTTC‑GCTCATATGCCGCAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                                                                     <  1:744113/239‑1 (MQ=255)                                                                                                                                                         tGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGTAAAGTAAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCAGCCGCTTGATTCATTTTGGTCCCACGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTtttg                                                                                >  1:1331581/1‑239 (MQ=255)                                                                                                                                                          gAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGTTCCATCCTGTGGAACAGATGC‑GGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTgg                                                                               <  1:1302335/239‑1 (MQ=255)                                                                                                                                                          gAAAACCGG‑CCCCAGTTTTTTGATTGGGTT‑ATCACCCTAAAACCTATAATAAAAAC‑GC‑AGGGA‑ACCGGTTTTA‑TTTTGTTGCTCACTCCTGTGAAACAGAGGC‑GGCGGTTGATGACCAGTCAATTAGGAAAAGTCAGCCAATCCTTC‑GCTCATATGCCCAAGGACGATCCGAAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTgg                                                                               <  2:1331581/239‑1 (MQ=255)                                                                                                                                                                cAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCACCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGggaaga                                                                         >  1:141040/1‑239 (MQ=255)                                                                                                                                                                      cccattttttGGTGCGTTGTATACCACTAAAACCGATAAGAGAAAC‑GA‑AGAGA‑ACCAGCTTTA‑TTTTTTTGCTCCACCCTGTGAAACAGGCGC‑GGCAGTGGATGGCCAGTCATTTAGGAAAAGCCAGCCATACCTGC‑GCTCATATGCCACAGGACGTCCCGCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGc                                                                     <  2:1061158/235‑1 (MQ=255)                                                                                                                                                                         gcttATTGAGGCACTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTCATGCTCCATCCGGTGGAACAGAGCC‑GGCAGTTGATGATCATTCAGTTAGGAAAAGGCAGCCCTACCTTG‑GACCATATGCCCCAGGACGCTCCGCAAACCCGCCACTTGACTCCTTTTGTACGCCAGTCGCCCAGCGTAACCATTCCTGTCCACCTTTGTGGGAAAGAgggggggg                                                                 >  2:1333924/3‑234 (MQ=255)                                                                                                                                                                               tgagtgggTG‑ATAACCCTTAACCCGATAATAGAAAC‑GC‑AGGAA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACATATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAgg                                                           <  1:1012700/233‑1 (MQ=255)                                                                                                                                                                                tgttggtgttattaCCCTTAAACCTAAAAAAAAAAC‑GG‑GGGAA‑CCCGGTTTTT‑TTTTGTGCCCCCACCCGGTGGAAAAGAGGC‑GGCAGTTGAGCACCAGACAATTAGAAAAAGCCCGCCATACCTTC‑CCTCATATGCCACAGGACGACCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAgg                                                           <  2:175526/226‑1 (MQ=255)                                                                                                                                                                                 tggtgggggtaataccTTAAACCCATAAAGAAAAC‑GG‑AGGGAGACAAGTTTTA‑ATTT‑TTGCGCCCTACCGTGGAACAAAGGCGGGGTGTTGATGACGAGTAAATTAGG‑AAAGTCCACCATTCCCTCGGTTAATGTGCCAAAGAACGGTCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAgg                                                           <  2:106683/225‑1 (MQ=255)                                                                                                                                                                                 aTGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGc                                                          >  1:669065/1‑238 (MQ=255)                                                                                                                                                                                   gCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGGGAAGAGGGCGTGGGAAAGGcccg                                                       >  1:676858/1‑236 (MQ=255)                                                                                                                                                                                    cGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACt                                                       >  1:314783/1‑238 (MQ=255)                                                                                                                                                                                    cGGCG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACCGGACGATCCGCAAACCCGCCGCCTGATTCATTTTGGTCCCCAGGCGGGCCGCCGTAGCGTTCCGTACCTACTTTGTGGAGAAAAGGGGGTGGGCCAGGAAcg                                                       >  2:467904/1‑237 (MQ=255)                                                                                                                                                                                     ggTG‑AAAACCCTTAAACCGATAATAGAACC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTGGCCCCATCCTGTGGACCAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTg                                                      <  1:754224/238‑1 (MQ=255)                                                                                                                                                                                      gTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                                                            >  2:634043/1‑183 (MQ=255)                                                                                                                                                                                       gttaTAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                                                            <  1:634043/180‑1 (MQ=255)                                                                                                                                                                                         ttattcccTTTAACCCAAAAAAGAAAC‑CCGAGGAA‑CCCATTTTTT‑TTTTGTTGCCCCATCCTGTGGAACAGAGTC‑GGCAGTTGATGCCCAGACAATTAGGAAAAGTCAGCCATCCCTTC‑GCTCATAGCCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCt                                                    <  2:770110/233‑1 (MQ=255)                                                                                                                                                                                               ctttAACCCGTTAATAGAAAC‑GG‑GGGGA‑ACCATTTTTT‑TTTTTTTGCCCCATCCTGGTGACAGGGTGG‑GGGAGTTGATCACCAGTCAATTAGGAAAAGTCAGCCATCCCTTC‑GCTCATATGCCACGGGACGATCCGCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAgg                                            <  2:676858/237‑1 (MQ=255)                                                                                                                                                                                               ccTTAACCCGATAATAGAAAC‑GC‑AGGGA‑ACCGGCTTTA‑TTTTGTTGCCCCATCCTGTGGAACAGATGC‑GGCAGTTGAGGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAgg                                            <  1:37324/239‑1 (MQ=255)                                                                                                                                                                                                  tAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGTTTTATTTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGagaag                                                                          <  1:951332/207‑1 (MQ=255)                                                                                                                                                                                                  tAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTATTTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGagaag                                                                          >  2:951332/1‑207 (MQ=255)                                                                                                                                                                                                  tAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCCTTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTTTGGGGAAAAGGGCGGGGCCAAGacacgtgacagccaggaaa                                         >  2:1161576/1‑224 (MQ=255)                                                                                                                                                                                                      aagagaataGAACC‑GG‑AGGTA‑TCCAGTTTTA‑TTTTGTTGCCCTAGCGTGTGGAACAGATGC‑GGCATTTGAGCACCAGTAAATTGGGAAAGGTCACCCATCCCTTC‑GCTCATATGCCACGGGACGTTCCGCAACCCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCa                                     <  2:255543/234‑1 (MQ=255)                                                                                                                                                                                                         ataataGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGTTCGCCAGGCGGCGCGCCGTAGCTTTCCTGTCCTGCTTTGTGGAGAAAGGGGGGTGGGCAAGGCACTGCTaaagcaagaaattacaaa                                  >  2:357258/1‑223 (MQ=255)                                                                                                                                                                                                         ataataGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGTTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTTGGGGAAAAAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCaaaa                                  >  2:977342/1‑239 (MQ=255)                                                                                                                                                                                                         ataataGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTTTGGAGAAGAGGGCGTGGGCAAGGCCCTGCTAAATCAGGCCATTCATaa                                  >  2:1092112/1‑239 (MQ=255)                                                                                                                                                                                                         ataataGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCCCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGGTGGCTGGGGCAAGGCACTGCTAAGTCAGGCAATTCCTaa                                  >  1:1210525/1‑239 (MQ=255)                                                                                                                                                                                                         ataataGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCCGCAGGCGCCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATaa                                  >  1:535483/1‑239 (MQ=255)                                                                                                                                                                                                         ataataGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑AGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACCGGACGATCCGCAAACCCGCCGCTTGATTCATTTTTGTCGCCAGGTGGCGCGCGGTACCTTTCCTGTCCTGCTTTGTGGGGAAAGGGGCGGTGGCAGGGCACTGCTAAGTACGGCAATTctaaa                                  >  2:1074851/1‑235 (MQ=255)                                                                                                                                                                                                                   c‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGCTTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGGGGGCAAGGCACTGCTAAGTCAGGCACTTAATAATGAAagcgag                        >  2:264658/1‑239 (MQ=255)                                                                                                                                                                                                                   c‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAagg                           >  1:255543/1‑235 (MQ=255)                                                                                                                                                                                                                         gggA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGGGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAaacgaggggg                    >  2:1085420/1‑235 (MQ=255)                                                                                                                                                                                                                          ggA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCCCCGCTTCATTCATTTTTGTCGCCACGCGGGGCCCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGGCCCTCTAAGGCAGGCAATTCTTAATGAAAACGCGCGggc                   >  2:1332852/1‑239 (MQ=255)                                                                                                                                                                                                                            a‑aCCAGTTTTA‑GTTTGTTGCTCCAACCGGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACg                                                                                                                                                        <  2:648262/104‑1 (MQ=255)                                                                                                                                                                                                                              aCCGGCTTTT‑TTTTGTTGCCCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCATGTAGGAAAAGTCAGCCATCCCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGtgctgc                <  1:1051268/239‑1 (MQ=255)                                                                                                                                                                                                                                   cTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCt                                                                                     >  1:1011815/1‑165 (MQ=255)                                                                                                                                                                                                                                       a‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAAc                                                                                                                                              >  1:648262/1‑104 (MQ=255)                                                                                                                                                                                                                                              ttgCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCCAACCCGGCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGGCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGAGTGCTGCAGGTCCTTATATCg  >  1:305741/1‑238 (MQ=255)                                                                                                                                                                                                                                              ttgCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCCAGGCCCTGCTAAGCCAGGCAATTCATAATGAAAACGAGCGGGCGGCAGGTCCTTATATcc  >  2:661254/1‑237 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            CAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCC‑TTGACGCGACGGCAAGT‑CAGGGACGGGCAATCACTGAACTCTTAACGTTA‑CCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAG‑CACCAGTTTATTGATGCGGTG‑ATAACCCTTAAACCGATAATAGAAAC‑GC‑AGGGA‑ACCAGCTTTA‑TTTTGTTGCTCCATCCTGTGGAACAGATGC‑GGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTC‑GCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCG  >  NC_000913/1251741‑1252206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑