Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,325,991	A→G	12.4%	S416G (AGT→GGT)	yciQ →	enhancer of membrane protein expression, putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,325,991	0	A	G	12.4%	191.3 / 2.2	83	S416G (AGT→GGT)	yciQ	enhancer of membrane protein expression, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (17/52);  new base G (8/3);  total (27/56)
Fisher's exact test for biased strand distribution p-value = 3.02e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTACTGGTATATGCCAGCGGG  >  NC_000913/1325753‑1326226                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    ttaagggacaggAAAAAATGGGGTTTTGCTGGCCGGAGGGCAGTTATTACGGGAAGCGTTATTTCGGAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  2:1197800/228‑1 (MQ=255) aggggggACCAAAAATAATGCGGTATTACCGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCA‑TTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  2:341777/232‑1 (MQ=255) aGGCGCGTCCAGAAATAATGCGGTATTTCTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  1:1094670/239‑1 (MQ=255) aGGCGCGACCAGAAATAATGCGGTATTGCTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  1:420651/239‑1 (MQ=255)  gggccgtccAGAAATAATGGGGTATTACTGCCCGGAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGGAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  2:964111/231‑1 (MQ=255)  ggCTCGACCAAAAATAAGGCGGTATTCCCGCCCGCAGAGCAGTTATTACTGGAAGGGTTATTTCGTAAAGGCGATAAGGTCGTGCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  2:789848/238‑1 (MQ=255)   gcccgagccgaacaaatGCGTTATTTCCGCCCGCAGCGCAGTTATTGCTGGAAGCGTGATTGCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGTCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACTGTAACTTGa                                                                                                                                                                                                                                                     <  2:1051386/223‑1 (MQ=255)       gACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAACAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGCCTGGCAGTATTGGCGGGCATTTTTTTCGGGAAATTGTGGCCtt                                                                                                                                                                                                                                               >  1:1378386/1‑238 (MQ=255)           cgaaattgaggtgTATTACTGCCCGCAGAGGTGTTATTCATGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGGGACGCAACAGAGTTTTACGCAGGGCATTTTTTCGGATGCAGAAATTTTTTC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAgg                                                                                                                                                                                                                                           <  2:1345177/227‑1 (MQ=255)              aattatGCGGTATTACTGCCCGCAGGGCAGTTATTACTGGAATCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGc                                                                                                                                                                                                                                          <  1:1035496/237‑1 (MQ=255)              aataatGCTGTATTACTGCCCGCAGAGTAGTTATTACTGGAAGCGGTATCTCGTAAAGGCGATAAGGTAGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATCTTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAgg                                                                                                                                                                                                                                           <  1:712855/236‑1 (MQ=255)                 tatGCGTTTTTACTGCCCGAGGGGCATGTATTACTGGAAGCGTTTTGTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                                                                                                                                                                                                     <  2:1159985/238‑1 (MQ=255)                 tatGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                                                                                                                                                                                                     <  1:21172/238‑1 (MQ=255)                   tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCGGTGAATTTGGTCCTGTAGGCTGGGCAg                                                                                                                                                                                                                                   >  1:82226/1‑239 (MQ=255)                   tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGCCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGCAATTTTTTC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTCTTTTCGTTAACTTGGGTACTGtggtggcgggag                                                                                                                                                                                                                                   >  2:1018019/1‑228 (MQ=255)                      ggTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAgg                                                                                                                                                                                                                                           <  1:459726/228‑1 (MQ=255)                       tgtttatttcccGGAGGGCAGTTATTACTGGAAGCGTTTTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGaa                                                                                                                                                                                                                                <  2:174883/229‑1 (MQ=255)                       gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTCTTTTCGGTCACTTGGGTCCCTTATGATGGGCGGGGAt                                                                                                                                                                                                                               >  1:1159985/1‑239 (MQ=255)                       gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTCGGTAACTTTGGTCCTTTTGGcctgtccctttt                                                                                                                                                                                                                               >  1:1197800/1‑228 (MQ=255)                       gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCGGTAACTTTAGGCCTTTAGGCTGGGCAGGAAt                                                                                                                                                                                                                               >  1:542603/1‑239 (MQ=255)                       gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGGCATTTTTTTCGGTAACTTGAGTCCTGTGGGCTGGGCCGGAAt                                                                                                                                                                                                                               >  1:238054/1‑239 (MQ=255)                               gCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCtgg                                                                                                                                                                                                                      <  1:97282/239‑1 (MQ=255)                                  cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCACTATTGGCGGTCATTCTTTACGGGTACTTAGGTCCTGTTGGCTGGGCAGGGATGA‑GTCtgggtg                                                                                                                                                                                                                   >  1:634105/1‑236 (MQ=255)                                  cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGGAGGA‑GGCtggtgg                                                                                                                                                                                                                   >  1:27792/1‑239 (MQ=255)                                        gcttttttttttGGAGGTGTTTTTTCGTAAAGGCGTTAAGGTCTTTCTTACGTGGAGACCTAACAGTGTCTTTCGCAGGGCATGTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGTAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGat                                                                                                                                                                                                              <  2:556157/227‑1 (MQ=255)                                        gCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGat                                                                                                                                                                                                              <  1:247555/238‑1 (MQ=255)                                        gCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGat                                                                                                                                                                                                              <  1:665050/238‑1 (MQ=255)                                        gCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGa                                                                                                                                                                                                               <  1:473967/237‑1 (MQ=255)                                          aGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAAATTGAGTCCTGTTGGCTGGGGAGGAATGA‑GGCTGGGGGGCGctatt                                                                                                                                                                                                           >  1:375078/1‑238 (MQ=255)                                          aGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGACTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACGAGCAATATTGGCGTGCATTTTTTTCGGTAAATTGGGTTCCCTGGGCCGGGGAGG‑ATGAGGTCTTGTggggggattg                                                                                                                                                                                                           >  2:1243188/1‑232 (MQ=255)                                           ttttttaATGGATGTGTTTTTTTGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGt                                                                                                                                                                                                          <  2:27792/235‑1 (MQ=255)                                            ttttttcTGGAAGCGTTTTTTAGTAAAGGGGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGGGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGt                                                                                                                                                                                                          <  2:82226/232‑1 (MQ=255)                                            ttattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  1:1211223/196‑1 (MQ=255)                                                      aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTTATTCTTTTATGTAAATTTGATCCTGTAGGCCGGGCAGGAATGA‑GACTGTGGGGCggaagggtttttttgt                                                                                                                                                                                                 >  1:518591/1‑222 (MQ=255)                                                      aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCACTCTTTCCGGTAACTTGAGTGCTTTATGCTGGGCCGGAGGTG‑GTCTTGTGGGGGAGAtttttt‑tttttt                                                                                                                                                                                                >  2:699715/1‑233 (MQ=255)                                                          tttttttCTTAAAGGCGATAAGGTTGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATctg                                                                                                                                                                                            <  2:1012977/233‑1 (MQ=255)                                                          gTTATTTCGTAAAGGCGATAAGGTCTTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATctg                                                                                                                                                                                            <  1:485584/237‑1 (MQ=255)                                                          gTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAAAAGAGCCTTACGCATGGAATTTTTACGGATGCAGAAAGTTTATC‑TGACGCGTAAAAAGTCTTCTTTTTACCGGTCTGATTCTTTTTTGAAATGGGGTGGAGTGGGAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAAGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATctg                                                                                                                                                                                            <  1:1133836/237‑1 (MQ=255)                                                               ttCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     >  1:148081/1‑177 (MQ=255)                                                               ttCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  2:148081/177‑1 (MQ=255)                                                                    aaaGGCTGTTAGGTCTTTTTTACGGGGAGACGCAACAGGGTTTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTcc                                                                                                                                                                               <  2:634105/239‑1 (MQ=255)                                                                        gCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGACATTCTTTACGGTAACTTGAGTCCTGGTGGCTGGGCCGGAATTA‑GGCTGGTGGGCGCATTGGTTT‑TTATGATCTGCTGG‑GTTATTCCtttt                                                                                                                                                                           >  1:393536/1‑239 (MQ=255)                                                                                 tCGTTTTTACGGGGAGACCCAAGAGATGTTTACGCAGGGTATTTTATCGGAAGCAAAAATTTTGTG‑TGCCGCGTAAAAAGTCGTCGTTTTGCCGGTCTGGTACGTTTTTGCAATGGTGTGGAGTGGGAAGATTGGCGGTCATTCTTTACGGTAGCTTGAGTCCTGTAGGCTGGGCAGGAATTA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTGTTTTATTTTg                                                                                                                                                                   <  2:457390/238‑1 (MQ=255)                                                                                  tgTTTTTACGGGGAGGCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑t                                                                                                                                                                 <  1:1351370/237‑1 (MQ=255)                                                                                  cGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTACGGGAACTTTAGTTCCGTAGGGTGGGGCGGAATGG‑GTCTGGTGGGCGGTTTGTTTA‑TTTTGATCTGTTGG‑GTTTTTCCTtttttttttttt                                                                                                                                                                  >  1:137367/1‑236 (MQ=255)                                                                                   tttcttcCGGGGAGACGCAACAGAGTTTTACGCAGGGCATTTTTACTGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTGACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑tt                                                                                                                                                                <  1:287445/237‑1 (MQ=255)                                                                                       tttcgggggagtggaaCAGATTGTTACGCAGTTCATTTTTACGGATGCGGAAATTTTCTC‑GGCCGAGTAAAAAGTCTTAGTTTTGCCTGTCTGATACTTTTTTGCCATGGGGTGGGCTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCtt                                                                                                                                                           <  2:1062324/225‑1 (MQ=255)                                                                                                 aCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGGCCTTCTTTTAGGTAACTTTAGTCCTGTAGGCTGGGGAGGAAAGG‑GTCTGGGGGGGGGTATGTTTT‑TTTTTTTTTGCTGG‑GGTTTTTCTTTTTTATTTTT‑TTTCCtttgtgtttttt                                                                                                                                                 >  2:1056856/1‑229 (MQ=255)                                                                                                     aaCAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTccc                                                                                                                                                             <  1:670731/223‑1 (MQ=255)                                                                                                           gttttACGCGTGGCTTTTTTACGGTTGCGGAAATTTTATC‑TGCCTGGTAAAAAGTTTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGATTCCTGTAGGCTGGGCAGGAATGA‑GTCTTGTGGGCGATATGTTTA‑TTATGAGCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCga                                                                                                                                       <  2:820323/236‑1 (MQ=255)                                                                                                           gTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCcgcg                                                                                                                                        <  1:1243188/238‑1 (MQ=255)                                                                                                             cTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGTGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTAGGTAACTTGAGTCCTGTAGGACGGGCAGGGATGA‑GTCTGGGGGGGGGTATGTTTA‑TTTTGATCTGCTGG‑GTTATTCCTTTTTTTTTTTT‑TTGCCTTGTGCTTTTGTTTTCCCGCgatg                                                                                                                                     >  1:82059/1‑239 (MQ=255)                                                                                                               tACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGGGGGGGATATGTTTT‑TTATGTTCTGCTGG‑GTTATTCCTTTTTTATTTTG‑TTCCCTTTGGCTTTTGTTTTCCCCCgctggt                                                                                                                                   >  1:1232472/1‑237 (MQ=255)                                                                                                                  gCAGGGCTTTTTTTCGGAGGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTGCCGGTCTGATGCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGAc                                                                                                                                <  2:393536/239‑1 (MQ=255)                                                                                                                  ccAGGGTATTTTTTCGGATGCAAAAATTTTATC‑TGCCGGGTAAAAAGTTTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTCTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGAc                                                                                                                                <  2:525296/238‑1 (MQ=255)                                                                                                                    aGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTTAGTCCTGTAGGCTGGGCAGGGATGA‑GTCTGTGGGGGGGTATGTTTA‑TTTTTTTTTGCTGG‑GTTTTTCCTTTTTTATTTTT‑TTTCCTTTGGCTTTTGTTTTTCCCCgttgattgcaa                                                                                                                              >  1:1313405/1‑234 (MQ=255)                                                                                                                      gggaTTTTTAGTGAAGCAGAAATTTTAGCATGATG‑GTAAAAAGTCTTTGTTTTACCGGTTTTATCCTTTTTTGCATGGGGGTGGATTGGCAATATTGCCGTTCATTCGTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTTGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTAGTTTG‑TTCCATTGAGCGTTTGTTTGCCCTCGATGATGACAAGc                                                                                                                            <  2:938930/236‑1 (MQ=255)                                                                                                                      ggCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTCGGTAACTTTTGTCCTGTAGGGTGGGCAGGGATGA‑GGCTGTTGGGCGATATTTTTA‑TTTTTGTCTGCTGG‑GTTTTTTCTTTTTTTTTTTG‑TTCCCTTTGGCttttttttttccccggagttggaaaa                                                                                                                             >  2:457626/1‑220 (MQ=255)                                                                                                                      ggCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCTGTAACTTGAAGCTTGTCGGCATGGCAGGGATGA‑CTCTGGGGGGCGATAGGTGTATTTTTTATCT‑CTGG‑GTTCTTTCTTTTTTTTTTTT‑TTTCGCTTCGGTTTTGTTTTTCcgcggcgtgtataag                                                                                                                             >  2:255897/1‑227 (MQ=255)                                                                                                                      ggCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGGAACTTTAGTCCTGTAGGCTGGGGCGGGATGA‑GGCTGGTGGGCGGTATGGTTT‑TTATGATCTTCTGG‑TGTATTCCTTTTTTTTTTTGTTTCCTTTGAGTTTTTTTTTccccccaggaagaagaa                                                                                                                              >  2:229154/1‑225 (MQ=255)                                                                                                                        cATTTTTACGGATGCATAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGcc                                                                                                                           <  1:1056856/238‑1 (MQ=255)                                                                                                                         ctttttccgggagcaatagttttATT‑CGCCGGGTAAAAAGTCTTCGTTTTACCGGTCTGGTACTTTTTTGCAATGGGGTGGTCTGGCAATATTGGCTGTCGTTCTTTTGGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCt                                                                                                                          <  2:651623/219‑1 (MQ=255)                                                                                                                         aTTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGGCTTCGTTTGACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCGTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCt                                                                                                                          <  1:842242/238‑1 (MQ=255)                                                                                                                                cGGATGCAGAAATTTTATT‑GGCCGGGTAAAAAGGCTTCGTTTGACCGGTCTGATAGTTTTTGGCAATGGGGTGGACTGGCAATATTGGTGGTCATTCTTTACGGTAACTTGAGCCCTGTAGGCTGGGCAGTAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTaa                                                                                                                 <  1:1218577/239‑1 (MQ=255)                                                                                                                                cGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTaa                                                                                                                 <  1:69487/239‑1 (MQ=255)                                                                                                                                                 attttgcttttAAAAAGTTTTCGTTTTACCGGGTTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACt                                                                                                <  2:82059/229‑1 (MQ=255)                                                                                                                                                           tAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                                                     <  1:350111/86‑1 (MQ=255)                                                                                                                                                             aaaaGTCTTCGTTTTACCGGTCTGATACTTTTGTGCAATGGGGTGGACTGGCAATATGGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑Ca                                                                                   <  1:783495/239‑1 (MQ=255)                                                                                                                                                                  tCTTCGTTTTACCGGTTTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGGGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGAt                                                                             <  2:365066/239‑1 (MQ=255)                                                                                                                                                                   cTTCGTTTTGCCGGTGTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGAt                                                                             <  1:511196/238‑1 (MQ=255)                                                                                                                                                                            aCCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGCCATTTTTTTCGGTTACTTGAGTTCCTTAGGCTGGGCCGGGAAGG‑GTCTGGTGGGCGTTTTGTTTT‑TTTTGATCTTCTGGTTTTTTTCCTTTTTTTTTTTT‑TTCCTTTTGGTTTTTTTTGGCCCCCG‑TGTTTGCAAGCCTTGCCGTTAAT‑GTTTAATTCTCCCCTTTGTTTTT‑C‑CACTTGATTTGtcaggg                                                                    >  2:531993/1‑234 (MQ=255)                                                                                                                                                                               ggTTTGATTCTTTTTTGGAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAAGGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTg                                                                <  1:12992/239‑1 (MQ=255)                                                                                                                                                                                 tCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGc                                                                                                                            <  1:278239/181‑1 (MQ=255)                                                                                                                                                                                    gATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACGTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTGTAC‑CAC‑TGATTTGTTCAGGCGTGGCCtt                                                           <  1:531993/239‑1 (MQ=255)                                                                                                                                                                                         ttttttTGAATGGGGTGGGTTGGCATTTTTGGGGTTCTTTCTTTACGGTAACTTTGGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGATAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTAtt                                                      <  2:542603/239‑1 (MQ=255)                                                                                                                                                                                         ttttttTCCATTGGGTGGACTTGGTATTTTTGCGGGGTTTCTTTACGGTACCGTGAGTCCTGTAGGCTGGGCAGGAATGA‑TTCTGTGTGGCGATATGTTTA‑TTATTATCTGCGGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TTATTTGTTCAGGCGTGGCCTTCTAtt                                                      <  2:433662/239‑1 (MQ=255)                                                                                                                                                                                         ttttttGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTAtt                                                      <  1:1018019/239‑1 (MQ=255)                                                                                                                                                                                         ttttttGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTAtt                                                      <  1:140284/239‑1 (MQ=255)                                                                                                                                                                                         ttttttGCAATGGGGTGGACTGGAAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTAtt                                                      <  1:425771/239‑1 (MQ=255)                                                                                                                                                                                         tttttgTCAGTGGGGTGGGATGGCAATATTGGGGGGTATTTTTTACGGTAACTTTGGTCCTGTAGGGTGGGCAGGAATGA‑GTTTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTAtt                                                      <  2:862397/239‑1 (MQ=255)                                                                                                                                                                                           ttttGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTTCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAACCTTG‑CGTTTATCGTGTAA‑TCATCACTTTTTTTTTAC‑CAC‑TGATTTTTTCAGGCGTGGCCTTCTTTtct                                                    >  1:373936/1‑239 (MQ=255)                                                                                                                                                                                               gCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTCGGTAACTTGAGTCCTGTAGGCTGGGCAGGGATGA‑GGCTGGGGGGGGATATGTTTA‑TTATTATCTTCTGG‑GTTATTTCTTTTTTATTTTT‑TTTCCTTTGGCTTTTTTTTTCCCGCGTTGATGACAAGCCTTT‑TGTTAATCTTGTAA‑TTATTCCTTTTTTTTTTC‑CCC‑TCTTTTTTTTTGGGGGGGGCTTTTTTTctctct                                                >  2:1076287/1‑239 (MQ=255)                                                                                                                                                                                                   tGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCTGTAACTTTGGTCCTGTAGGGTGGGCAGGGATGA‑GTGTTGTTGGGGGTATGTTTA‑TTTTGATCTGCTGG‑GTTATTCCTTTTTTTTTTTT‑TTCCCTTTCGCTTTTTTTTTCCCGCTGTGTTGACAAGCCTTG‑CGTTATTCTTGTTA‑TCATCACTTTGTTTTTACACAC‑‑GATTTTTTTAGGCGTGGCttttatttttctctcat                                             >  2:140284/1‑224 (MQ=255)                                                                                                                                                                                                   tGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACAt                                            <  1:1076287/239‑1 (MQ=255)                                                                                                                                                                                                   tGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACAt                                            <  1:1130037/239‑1 (MQ=255)                                                                                                                                                                                                              ggCATTTTTGGCGGTCATTTTTTACGGTAACTTGGGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGgag                                 <  2:373936/239‑1 (MQ=255)                                                                                                                                                                                                                  atttttgcgtgctttttttACGGTAACTTGAGTCCTGTAGGCTGGGGAGGAATGG‑GTCTGGTGGGCGATATGTTTA‑TTATTATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGt                             <  2:137367/223‑1 (MQ=255)                                                                                                                                                                                                                       ggCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCt                        <  1:640718/239‑1 (MQ=255)                                                                                                                                                                                                                            tCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTa                    <  1:255897/238‑1 (MQ=255)                                                                                                                                                                                                                             cATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAc                   <  1:457626/238‑1 (MQ=255)                                                                                                                                                                                                                             cATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAc                   <  1:229154/238‑1 (MQ=255)                                                                                                                                                                                                                                             ttGTGTCTTGTGGGCTTGGCAGGAAGTG‑GTTTGGTGGGGGATTTGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTACTGGTATATGCCAGCggg  <  2:1232472/239‑1 (MQ=255)                                                                                                                                                                                                                                              tGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CCC‑TGATTTGTTCAGGCGTg                                                                >  2:1221662/1‑176 (MQ=255)                                                                                                                                                                                                                                              tGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTg                                                                <  1:1221662/176‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    AGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATC‑TGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGA‑GTCTGGTGGGCGATATGTTTA‑TTATGATCTGCTGG‑ATTATTCCTTTTTTATTTTG‑TTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTG‑CGTTAATCGTGTAA‑TCATCACTTTGTTTTTAC‑CAC‑TGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTACTGGTATATGCCAGCGGG  >  NC_000913/1325753‑1326226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑