Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,435,704 G→A 13.2% intergenic (‑85/+56) uspF ← / ← ompN stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,435,7040GA13.2% 219.3 / 2.4 87intergenic (‑85/+56)uspF/ompNstress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (62/11);  new base A (5/8);  total (68/19)
Fisher's exact test for biased strand distribution p-value = 9.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCT  >  NC_000913/1435467‑1435928
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     
aCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACGCTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGa                                                                                                                                                                                                                                      >  1:448160/1‑239 (MQ=255)
aCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGa                                                                                                                                                                                                                                      >  1:501339/1‑239 (MQ=255)
aCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTACCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGAAACTTTTTGTGTAATGACGGAGTTCAAATTTTTAATTTAGa                                                                                                                                                                                                                                      >  2:557196/1‑239 (MQ=255)
    aaggaaaaaTAGTAGGGCAGTGAATGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTTTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACGCTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCaa                                                                                                                                                                                                                                  <  1:988053/231‑1 (MQ=255)
     gagagGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCaaa                                                                                                                                                                                                                                 >  1:1050552/1‑239 (MQ=255)
      agagGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAag                                                                                                                                                                                                                                >  1:916756/1‑239 (MQ=255)
           cATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTTGTTATTGCCGGGGTTCACATTTTTAATTTAATTAAAAggagga                                                                                                                                                                                                                          >  2:311002/1‑239 (MQ=255)
           cATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAggagga                                                                                                                                                                                                                          >  1:383115/1‑239 (MQ=255)
           cATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATAAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGCTAGGGACAAGATTCGTTCTGTTCATAAAACCTCCAGTTTTAGTATCCGCATAAAGAGTAACGCCAGATGACCATTTTTGGGTATGAACGGGGTTACAATTTTTAATTTAGATCAAAggagga                                                                                                                                                                                                                          >  2:647909/1‑239 (MQ=255)
           actggtagGGCAGTGGAGGTATTACCGTCAGTAAAAGAACCTCTACAACATCTAGCTTTGCCTCTTCCTCAACGTGGGTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTGCACATTTTTAATTTAGATCAAaggagg                                                                                                                                                                                                                           <  1:1033168/234‑1 (MQ=255)
                    gCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAga                                                                                                                                                                                                                 >  2:372569/1‑238 (MQ=255)
                     cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCCTAAAACCTCCTGTTTTAGTATCCGCATAAATGGTAACGCCAGATAAAACTTTTTGGGTAATGCCGGAGATCAAATTTTTAATTTAAATAAAAGGAGGaaaaaaaaaaa                                                                                                                                                                                                                >  2:319691/1‑236 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGACATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa                                                                                                                                                                                                             >  1:789027/1‑239 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCCTAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCACAGGAGGAAGAATAAgaagaa                                                                                                                                                                                                             >  2:841421/1‑239 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGaaaaaaaaaacaaa                                                                                                                                                                                                             >  2:1314900/1‑233 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa                                                                                                                                                                                                             >  1:159011/1‑239 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGGGTAATTACGGACTTAACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa                                                                                                                                                                                                             >  1:599472/1‑239 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGAAAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTAAGTATCCGCTTAAAGTGAAACGCCGGATGACCCTTTTTGTGTAATGACGCAGTTAACATTTTTACTTTTAATAAAAgaaggagaaagtgtttctt                                                                                                                                                                                                             >  2:988053/1‑226 (MQ=255)
                        tGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCGTCCTCAACGTGGCTAATAACGCGTTGAGTTAATTCTGAATCGGGAATATCGCTAGGGCCAAAACATCTACTGTTCATAAAACCCCCTGTTTGAGTACCCGCATAAAGTGTACAGCCAGTTAACACTTTTTGTTCACTGACGAGGTTCCACTTTTTCTTTTAAATCAACGGAGGAAGAAAAAGCAGaa                                                                                                                                                                                                             >  2:1057169/1‑239 (MQ=255)
                          aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGACTCTTCCTCAACGTGGCTAATCACGCGTTGCGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTAATAAAACCTCCTGTTTTAGTCTCCGCATAAAGTGTAACGCCAGATGACCCTTTTTGTGCAATGACGGAGTTCACATTTTTAATTTATATTAAAGGAGGAAAAAAAAgaaatgga                                                                                                                                                                                                           >  2:1033168/1‑232 (MQ=255)
                                       cAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAAc                                                                                                                                                                                          >  1:1105184/1‑239 (MQ=255)
                                        aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAAGGACGGAGTTCACATTTTTAATTTAAATCAAAGAAGGAAAAAAAAACAGAAAA‑‑AAACCGcaaaaaaa                                                                                                                                                                                         >  2:25439/1‑232 (MQ=255)
                                        aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGAATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAACAGAAAA‑‑AACCCGCCATaaaa                                                                                                                                                                                         >  2:929561/1‑237 (MQ=255)
                                          gAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGATTAAGCAGAAAA‑‑AGCCCGCCATAACAGc                                                                                                                                                                                       >  1:1216177/1‑239 (MQ=255)
                                             aTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGATTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCa                                                                                                                                                                                                                                   <  2:693883/197‑1 (MQ=255)
                                             aTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGTTCa                                                                                                                                                                                                                                   >  1:693883/1‑197 (MQ=255)
                                                  ccttcgCATCACAATCTTTTGCCTCTTCCTAAACGGGGTTAACCCCGGTTGGAGTTAATTTTAAAGGGAAAAATTCGAAGGGAAAAAAATTGTTTCTGTTAAAAAACCCTCCTTTTTTATTATCCCAAAAAATTGTAACGCAAGATGACATTTTTTGTGTAATGAAGGAGTTCACATTTTTAATTTAGAAAAAAGGAGAAAAAAAAAGAAAAAAA‑‑AGCCCGCCATAACAGCGGGCagga                                                                                                                                                                               <  1:1314900/234‑1 (MQ=255)
                                                     ctGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAACTATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGGGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACACCGGGCAggagga                                                                                                                                                                            >  1:891971/1‑239 (MQ=255)
                                                      tGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGAt                                                                                                                                                                           >  1:127687/1‑239 (MQ=255)
                                                      tGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCGGTTTTAGTATCCGCATAAAGAGTAACGCCAGTTGAACCTTTTTTGGTAATGACGGAGTTCACATTTTTAATTTAGATATAAGGAGGAGGAATAAGGAGAAAA‑‑AGCCCGCCAGAACAGCGGGCagcgggct                                                                                                                                                                           >  1:688068/1‑233 (MQ=255)
                                                          tcatcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGTGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAAAAGCGGGCAGGCGGATTTAg                                                                                                                                                                       >  1:657972/1‑239 (MQ=255)
                                                             tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGa                                                                                                                                                                      >  1:413481/1‑237 (MQ=255)
                                                                            ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGCCAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGACGAATAAGAAGAAAA‑‑AGCCCGCCATAAAAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc                                                                                                                                                      >  2:287996/1‑239 (MQ=255)
                                                                            ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGGGGGCAGGAGGATTTaaatcagtaaaaacaaaac                                                                                                                                                      >  2:478160/1‑221 (MQ=255)
                                                                            ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCCCCATAACACCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc                                                                                                                                                      >  1:1293941/1‑239 (MQ=255)
                                                                                    cGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTACTATCCGCATAAAGCGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGCTCAACGTAGGAAGAATAAGCAGACAA‑‑AGCCCGCCATAACAGCGGGAAGGAGGATTTATAACGAAAAAACAAAACCAAAACCGa                                                                                                                                              >  2:323782/1‑239 (MQ=255)
                                                                                        gCTAATCACGCGTTGAGTTAATTCTGAATCGGTAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAAACTCCTGTTTTAGTATCCGCATAAAGGGTAACGCAAGATGACACTTTTTGCGTAATAACGGAGTTCACATTTTTAATTTAGATTAAAGGAGGAAGAATAACCGGAAAA‑‑AGGCCGCCATAACACCGGGCCGGAGGCATTAGAAAGGAAAAACCCAACCAAAAAGGACAat                                                                                                                                          >  2:427218/1‑239 (MQ=255)
                                                                                        gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAAAAGCGGGCAGAAGGATTTAGAACTGATAAACCAAACCTAAATCGACAat                                                                                                                                          >  1:170404/1‑239 (MQ=255)
                                                                                                   gTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTATTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTATTGACGGAGTTCACATTTTTACTTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATACCACCGGGACGGAGGATTTAGAACTGATAAACCACACCTAAAGCGAAAATACAATCGGTa                                                                                                                                >  1:362377/1‑238 (MQ=255)
                                                                                                   gTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAAAACTGAAAAACCAACCCAAAACCGACAaaatacccggaaa                                                                                                                               >  2:73320/1‑230 (MQ=255)
                                                                                                      gAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAgaga                                                                                                                            >  1:1282860/1‑239 (MQ=255)
                                                                                                                                    ggACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCt                                                                                               >  1:1333550/1‑238 (MQ=255)
                                                                                                                                     gACAAGAATCGTTCTGTTCATAAAACCTCCTGGTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGCGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGCGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatca                                                                                                                                      >  1:1192307/1‑198 (MQ=255)
                                                                                                                                         aGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATcc                                                                                         >  1:684136/1‑239 (MQ=255)
                                                                                                                                                gTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAAAAGCTGTCATCTTCATCCAACAgg                                                                                   >  1:194325/1‑239 (MQ=255)
                                                                                                                                                gTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAAAGATTCCATTGGCAGCGTAGAACATGCCAAATTAATCCaaaaag                                                                                   >  2:564823/1‑235 (MQ=255)
                                                                                                                                                gTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGAAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGGGATGCAATTGGCAGCCAAGAAGCTGTCATCTTCATACAACAgg                                                                                   >  1:601960/1‑239 (MQ=255)
                                                                                                                                                  ttCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCACCAGGt                                                                                  >  1:4304/1‑239 (MQ=255)
                                                                                                                                                    cTGTTCATAAAACCTCATGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTAACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGACCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTGCATCCAACCGGCTg                                                                                >  1:292442/1‑239 (MQ=255)
                                                                                                                                                       ttCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaa                                                                                                                                           >  1:1299409/1‑177 (MQ=255)
                                                                                                                                                       ttCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGGTGAtt                                                                             >  2:1266257/1‑239 (MQ=255)
                                                                                                                                                       ttCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGGAGAAGCTGTCATCTTAATCCAAAAGGtgaaat                                                                             >  2:427171/1‑234 (MQ=255)
                                                                                                                                                       ttCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAAGGATGCCATTGGACGCGTAAAACCTGTCACCTTCATCAAACAGGTTGAtt                                                                             >  2:267100/1‑239 (MQ=255)
                                                                                                                                                       ttCATAAAACCTACTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAACGACAATATCATCGATAGAGATGCCATTAGCAGCGTAAAAGATGAACTCTTCAACCAACAGGGTGAtt                                                                             >  2:508824/1‑239 (MQ=255)
                                                                                                                                                         aaTAAACCCTCTTTTTTTAGTACCCGAATAAGGTGTACCGCAAGAGGACATTTTTTGTGAAATGACGGAGTTCACATTTTAAATTTAGATAAAGGGAGGAAAAAAAAGAAAAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGa                                                                                                                                                                      <  1:175182/147‑1 (MQ=255)
                                                                                                                                                                 ctcctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAAAAGCTGCAATATTACTCAAACAGGTTGAtttttaatctaa                                                                   >  2:937782/1‑231 (MQ=255)
                                                                                                                                                                 ctcctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGGAAAAGCTGTCATCTTCATACAAAAGGTGAATTTTATAATCaa                                                                   >  2:699728/1‑239 (MQ=255)
                                                                                                                                                                 ctcctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGAAGAAGATGTCATATTCATCCAAAAGGTTGAATTTATAGTaaa                                                                   >  2:431015/1‑239 (MQ=255)
                                                                                                                                                                   cctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                                                                                                                            >  2:175182/1‑148 (MQ=255)
                                                                                                                                                                    ctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCaa                                                                                       >  1:818669/1‑216 (MQ=255)
                                                                                                                                                                    ctGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGt                                                                >  1:35653/1‑239 (MQ=255)
                                                                                                                                                                                 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtt                                                   >  1:187176/1‑239 (MQ=255)
                                                                                                                                                                                 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGGGGACAGGtt                                                   >  1:398327/1‑239 (MQ=255)
                                                                                                                                                                                      aaaattgaaaccgagagagcacTTTTTGGGGAAAGGAGGGATTCAATTTTTTAATTTAAAAAAAAGGGGAGAAAAAAAAGCAAAAAA‑‑ACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTa                                               <  1:427171/220‑1 (MQ=255)
                                                                                                                                                                                        agtaaacctccagatccattttttgGTGTAAGGACGGAGTCTACATTTTTGATTAAAATAAAAGGAAGAAAAAAACACAGAAAA‑‑GGCCCGCCATAACAGCTGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTATAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTg                                            <  1:287996/219‑1 (MQ=255)
                                                                                                                                                                                                  cAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAg                                  >  1:1158779/1‑239 (MQ=255)
                                                                                                                                                                                                   aattatatttttttGTTAAATTAAGGATTCAATTTTTTAAAGTAAAGAAAAGGGGGAAAAAAAAAAAAAAAA‑‑ACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGt                                 <  2:4304/230‑1 (MQ=255)
                                                                                                                                                                                                    gATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAATGGAGGCAGAATAAGCAGAAAA‑‑AGCCCGCCATAATAGCGGGCAGGAGGATTTAGACCTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGCGGACATGTTTTTATTGAAATAGTAAGTc                                >  1:1236475/1‑239 (MQ=255)
                                                                                                                                                                                                    gATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAAAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCACAAGGTTGATTTTATAGTCAACGTCGGTGGAAGTGTTTTTTTTaaaaaaaaaagtg                                >  2:977908/1‑230 (MQ=255)
                                                                                                                                                                                                            tttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCaa                                                                                                                                                                                                                                  <  1:1153389/42‑1 (MQ=255)
                                                                                                                                                                                                            tttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCaa                                                                                                                                                                                                                                  >  2:1153389/1‑42 (MQ=255)
                                                                                                                                                                                                              tttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAATTCGCGCCAatat                      >  1:1335539/1‑239 (MQ=255)
                                                                                                                                                                                                              tttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGGGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAata                       >  1:409629/1‑238 (MQ=255)
                                                                                                                                                                                                                 ttaaaaagggggttttcaatttgttaattaaGAAAAAGGGAGAAAAAAAAAGAAAAAAA‑‑GGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCgg                   <  2:1282860/216‑1 (MQ=255)
                                                                                                                                                                                                                    tAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGGGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGt                >  1:694489/1‑239 (MQ=255)
                                                                                                                                                                                                                      agagcgagtgtaatttttttAATTAAGAAAAAAGGAGGAAAAAAAATAAGAAGA‑‑CGCCCGGCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGATAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAt              <  2:116161/224‑1 (MQ=255)
                                                                                                                                                                                                                      aTGAGGGAGTCCACTTTTTAAATTAAAATCAAAGGAAGAAGAAAAAGCAGAAAA‑‑ACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAt              <  1:372569/239‑1 (MQ=255)
                                                                                                                                                                                                                      aTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGGGGACATGTTTTTATTTAAATAGTAATTCGCTCCAAAATAGCCGTAt              >  2:185878/1‑239 (MQ=255)
                                                                                                                                                                                                                       tggcggagtcattttttttAATTTAAAACAGAGGAAAAAAAAAAAAGAAAAAA‑‑ACCCACGCCATAACAGCGGGCAGGGTGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAt              <  2:127687/225‑1 (MQ=255)
                                                                                                                                                                                                                           tgatttcaattttttAATTTAGAAAAAGGGGAGAAAAAAAAAAAAAAAA‑‑CGCCCGCCATAACAGGGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGGCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc         <  2:413481/229‑1 (MQ=255)
                                                                                                                                                                                                                           ggatttaaaaTTTTAAATTTAAAACAAGGGAGAAAGAAAAAGCAAAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTTATAAACCAGTCCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTTGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAAc         <  1:319691/230‑1 (MQ=255)
                                                                                                                                                                                                                            gAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTAAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTTGCGCCATTATCGGCGAATTTAAcc        >  1:925518/1‑239 (MQ=255)
                                                                                                                                                                                                                              tcacacaTTTTAAATTTAGCAAAAAGGGGAAAAAAAGAAGAAAAACAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTTGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCa       <  1:427218/235‑1 (MQ=255)
                                                                                                                                                                                                                              gTTCAAATTTTAAATTGAGAACAAGGGGAGAAAAAAAAAAAAAAAA‑‑CCCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCa       <  1:699728/238‑1 (MQ=255)
                                                                                                                                                                                                                              atctattttttttATTTAAAGAAAAGGGAGAAAAAAAAAAAAAAAA‑‑ACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCa       <  2:1275353/231‑1 (MQ=255)
                                                                                                                                                                                                                               ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGa     >  1:1275353/1‑239 (MQ=255)
                                                                                                                                                                                                                                  acaTTTTTAATTTGAAGAAAAGGAGGAAAAAAAAGAAAAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCt  <  1:478160/239‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     
ACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAA‑‑AGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCT  >  NC_000913/1435467‑1435928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: