| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,508,134 | A→C | 21.9% | V36G (GTG→GGG) | fryB ← | putative enzyme IIB component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,508,134 | 0 | A | C | 21.9% | 126.4 / 5.9 | 73 | V36G (GTG→GGG) | fryB | putative enzyme IIB component of PTS |
| Reads supporting (aligned to +/- strand): ref base A (36/17); new base C (5/12); total (42/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.59e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGT > NC_000913/2507898‑2508366 | ttATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGCCAGGCGATTCTGG‑ATACCGTCCCGGCCCTGGGGTTCAATTTTCACt > 2:709552/1‑239 (MQ=255) cccTTTCTAATTTTTCTTTTTTTCAGAGGCAATCCTCTCTTCGATTTTTTTGATGATGCCCGCAGCGTTTTTAATTGCT‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCAt < 2:397102/239‑1 (MQ=255) ctttCGAATTTTATTTTTTTTCAGGGGCAATTTTTCTTTCGATTTTTTTGATGATTCCCGGAGGTTTTTTAATTGCG‑TTCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTTGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATaa < 2:417004/236‑1 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAACCGTTCGTTATCTTCAGGGGTAACTGCCACGGAGTGGATGATGATGGTCGATCCGGCGATATCCTGCGCCGTCAGCCGCTTCTGG‑ATACGGTCCGCGCCCTGGGCTTCAATTTTAGCTTCAGaaac > 1:424720/1‑237 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGTTATCCTGCGCCGTCAGTCGATTCTGG‑TTACCGTCCGCGCCCTGGGTTTCAATTTTAACTTCCTac > 1:957098/1‑236 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCTTAAcc > 1:113865/1‑239 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAAcc > 1:828840/1‑239 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAAcc > 1:148244/1‑239 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAAcc > 1:180679/1‑239 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCAAAAcc > 2:841358/1‑239 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGCCAGGCGATTCTGG‑ATACAGTCCGCGCCCTGGGTTTCCATTTTCACTTCTTAAcc > 1:44817/1‑239 (MQ=255) gTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCCCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGCCGCTTCGGCGATATCCTGAGCCGTCAGGCGATTTTGG‑ATACCGTCCGCTCCCTGGGTTTCAATTTTCCCTTCTTAAcc > 1:43607/1‑239 (MQ=255) tCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATAGCCTGCGCCGTCAGGCGATTCTGG‑TAACCGTCCGCGCCCTGGGTTTCAATTTTCCCTTCAAAACCg > 2:392423/1‑239 (MQ=255) tCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGCCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCTTAACCg > 1:397102/1‑239 (MQ=255) acagatatTACTGTTGGTCAGAGGCAATCATCGCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGGCGCGTTATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAAc < 1:902466/233‑1 (MQ=255) cTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCCATTTTCCCTTCATAACCGG‑CTTCTA‑cc > 1:222551/1‑238 (MQ=255) gttCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAACCGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCCCTTCATAACCGG‑CTTCTA‑CCCCCGct > 1:253153/1‑239 (MQ=255) gttCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGTTGGTCGCTTCGGCGATTTCCTGCGCCGTCAGGCGGTTCTGG‑TTACCGTCCGCGCCCTGGGTTTCAATTTTTCCTTCTTAAACGG‑CTTTTA‑ccccgtc > 2:902466/1‑233 (MQ=255) gttCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGct > 1:70129/1‑239 (MQ=255) ttCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGctt > 1:117543/1‑239 (MQ=255) ttCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGct > 1:207262/1‑238 (MQ=255) cAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACAGG‑CTTCTA‑CCGCCGcttct > 1:292423/1‑239 (MQ=255) agGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTTCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCttcttc > 1:555782/1‑238 (MQ=255) agGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGGCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTAACTTCATAACCGG‑CTTCTA‑CCGCCGCTCCTTcc > 1:1088394/1‑239 (MQ=255) ggtattcttcttttggtttttttTGATGGTGCCCGAAG‑GTTTTTTATTGGGTTCCTGAAAGGTGATTCCATAAACGGCGCGTGATTCGAAGCGTTCTTTATCTTCCGGGGTACCTGCCACGGAGGGGATGAGGATGGTCGCTTCGGCTATACCCTGCGCCGTCAGGCGATTCTGG‑ATCCCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTcc < 2:148244/221‑1 (MQ=255) atcatcTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAgcg > 1:392152/1‑238 (MQ=255) atcatcTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAgcc > 1:423143/1‑237 (MQ=255) ttcttttgttttCTTTGATGGTGCCCGGAGCGTTTTTAATTGCG‑TCCTGAAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATCCCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGa < 1:353089/230‑1 (MQ=255) ctctTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCTTAACCGG‑CTTCTA‑CCGCCGCTTCTTCCCGCGCCTGa > 1:80504/1‑238 (MQ=255) ttCGATTTCTTTGATGATGCCCGCAGCTTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAACCGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCgg < 1:3326/239‑1 (MQ=255) gATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCCCCCCTTCTTCCACCCCCTGAGCGGccc > 2:545755/1‑238 (MQ=255) cTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCGGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCCTCTTCCAGCGCCTGACCGCCCATaaaa > 2:284291/1‑238 (MQ=255) ggttgttcgcgctgggttttttAATTGCG‑TCCGGAAAAGTGGTTTCATAATGTGCGGGTGATTAGAACGGTCGGTTACCTCCCGGGGTAACTGCCACGGAGGGGATGATGATGGGCGCTTGGGCGTTATCTGGCGCCGTCAGGCGATTCGGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTAATAACCGG‑TTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGgtg < 2:828840/222‑1 (MQ=255) cccGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCCTAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGCGTGACCCa > 1:179071/1‑238 (MQ=255) cGTTTTTAATTGCG‑TCCTGCAAGGTGATTTCAAAAACGGCGCGTGATGCGAAACGTTCGTTATCTTCCGGGGTAACTGCCAGGGAGGGGATGATGATGGTCGCTGCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCAt < 1:392423/238‑1 (MQ=255) tAATTGGG‑TCCTGAAAAGTGATTTCAAAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCCCTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGggcag < 1:709552/239‑1 (MQ=255) tGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGcac > 1:35346/1‑239 (MQ=255) tttCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGtt > 1:123825/1‑237 (MQ=255) tttCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTCCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTTGGGGCCAGGCCCTTCGGGCAGGACCATAAGGCAATCAGgttt > 2:242432/1‑238 (MQ=255) aaaGGTCGCGTGATTCAAAACTTTCTTTATCTTCGGGGGTAACTGCCGCGGGGTGGATGATGATGGTCGTTTCGGCGATTTCCGGCGCCGTCAGGCGATTCTGG‑ATCCCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTc < 1:688477/239‑1 (MQ=255) cgcgTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGtt > 1:889313/1‑239 (MQ=255) cgTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATATAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTCCTCATCTTTaa > 1:704604/1‑239 (MQ=255) gAAGCGTTCGTTAACTTCCGGGGTAACTGCCCGGGGGTGGATGATGATGGCCGTTTCGGGGATTCCCTGCGCCTTCAGGCGATTCTGG‑ATCCCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGt < 1:810811/239‑1 (MQ=255) tcgttATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCCGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCCGTGa > 1:897522/1‑239 (MQ=255) gcatgggAAACTGCCACGGACGGGATGATGATGGTCGCTTCGGCGATAGCCTGCGCCGTCAGGCGATTCTGG‑ATAACGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCGATAAGGTGTGAACCAGACCCAACGGGCCGGCACCTAAGGCCACACGTTCCTTACTCATCGTTCATCACCCGTTCCAGGATAGGTGACg > 2:579274/5‑239 (MQ=255) aCGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCCTAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACCTAAGGCAATCAGTTTCTTACTCCTCGTTAATTCCTCGTCCAGGGATATTTTACGGGGGAAATTATGacc > 2:877624/1‑237 (MQ=255) gtgatgaggatGTTCTCTTCGGCGATATCCTGCGCCGTCAGGCTATTCTGG‑ATCCCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCAA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCa < 1:841358/236‑1 (MQ=255) ggTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCCGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCCGTGATAGTTGAAGAGGGAAATTATGAAAGGGCAAAACAAATCGcc > 2:577374/1‑239 (MQ=255) gTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATcccct > 1:722158/1‑238 (MQ=255) tCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGt > 1:1014440/1‑239 (MQ=255) tCGGGGATACCCTGCGCGGTCGGGCGTTTCGGG‑ATCCCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑TTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:545755/239‑1 (MQ=255) ggggtttttccggctccggcAGGCGA‑TCTGGTATCCCGCCCCCTCCCTGGTTTTAATGTTTCCCTAAAAAACCGC‑CTTCTC‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAg < 2:70129/231‑1 (MQ=255) cggcttttCTGG‑TTTCCGTCCGCGCCCTGGGTTTAAATTTTCATTTCATACCCGG‑TTGCTA‑CCGCCGCTTCTGCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTgg < 2:180679/233‑1 (MQ=255) aTTCTGG‑TACCCGGCCCCGCCCGGGGTTACAATTTCCTCTTCAACGCGGC‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt < 1:503285/239‑1 (MQ=255) gcctccGTCCGCGCCGGGGGTTCAAATTTTCCCTTATAAACCGG‑CTTCTC‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGGGTGAGCCAGGCCCATCGTGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 2:1021030/235‑1 (MQ=255) gcccgcccccctgtcttGGGTTTCTGTTTTCACCTCAAACCCGT‑TTTCAA‑CCGCCGTTTCTTCCCGCGCCTGACCGGCCATAAAGGTGTGAGCCAGGCTCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 2:424720/223‑1 (MQ=255) gagcccGGCCGCGCCTGGGGTTACAATTTCCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCAGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:877624/235‑1 (MQ=255) tccccccccccccttGGTTTTCATTTTTCCCTTCAAACCCGG‑TTTCAC‑CCGCCGCTTCTTCCAGCGCCTGGGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg < 2:1014440/225‑1 (MQ=255) tcccccccGCCCCGTGGGTTTAATTTTTCCCTATAAACCGGG‑TTTTTC‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGGGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg < 2:207262/233‑1 (MQ=255) ctccGTCCGCGCCCGGGGTTCCATTTTCCCCTTCATACCCGT‑CTTCTC‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg < 1:564067/237‑1 (MQ=255) ccagttCCCCGCCCTGGGTTCCAATTTGCGCTTCATCCCCGT‑CTTCTA‑CCGTCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTTAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:519871/232‑1 (MQ=255) atcccccccgccccTGGGGTTTAAATTTCACCTCAAAACCCG‑CTTTTACCCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTTGGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 2:35346/227‑1 (MQ=255) ccggTCCGCGCCCGGGGTTTCAATTTTCCTTTCATACCGGG‑TTACAC‑CCGCCTCTGCTTCCAGCGCCTGAGCGGCCATAAAGGTGTTAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg < 1:284291/235‑1 (MQ=255) cccGGCCGCCCCTGGGGTTACATTTTTCCATTAAGAAGCGG‑TTTATC‑CCGCCCCTTCTTCCGGGGCCTGGGGGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTAGTTCAGTGATAGTTGACGAGGGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg < 2:407056/237‑1 (MQ=255) cGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACCTAAGGCAATCAGCTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGTACTTATGAACGTGCAAAACAATTCGCCGTTACCAGCCCAATAATGCATTTACTGCAGAATTAATTCCGCtt > 1:508680/1‑238 (MQ=255) cGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGAATTTACTGGAGAATTAATccc > 1:692373/1‑235 (MQ=255) tCCGCCCCCTGGGTTTCAATTTTCCCTTCAAAACCGG‑CTTCTC‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTa < 1:880968/239‑1 (MQ=255) ccccggcCCTGGTTTTCATTTTTCCCTTCATACCGGG‑TTTCAC‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTa < 2:123825/234‑1 (MQ=255) gcccccTGGGTTTAAATTTTCCCTTCAGACCCGT‑CTTCCA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg < 1:242432/236‑1 (MQ=255) gggTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGACGTTAACGGACAAAAAATGCATTTACTGGAAAATTAATCCCGCTGTACGGacggtc > 2:519871/1‑233 (MQ=255) ggTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCGGTACGGAAggggga > 2:762121/1‑234 (MQ=255) ttCCATTTTTCCCTCAAAACCGGTATTCCA‑CCCCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGGGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACg < 2:44817/239‑1 (MQ=255) aTTTTCACTTCATACCCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGt < 1:577374/239‑1 (MQ=255) | TTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCG‑TCCTGCAAAGTGATTTCATAAACGTCGCGTGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGG‑ATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGG‑CTTCTA‑CCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGT > NC_000913/2507898‑2508366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |