Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,511,426 G→C 15.8% intergenic (+101/+40) ypeC → / ← mntH DUF2502 family putative periplasmic protein/manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,4260GC15.8% 125.6 / 2.8 60intergenic (+101/+40)ypeC/mntHDUF2502 family putative periplasmic protein/manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base G (31/18);  new base C (4/7);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAC  >  NC_000913/2511192‑2511657
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       
gTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCCCCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGTTGCGCCCCTGGTGCGTCTTATCAGGCCCACCAACCGGCCCTTTAACCATGAGGGGGGGACAGGGGTTTAcgcccc                                                                                                                                                                                                                                     >  2:1088431/1‑237 (MQ=255)
                         ccaccGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGGACCGCCATTAAACGCGCCCCCCAATGGAGCACAATGCCTGATGCGACGCTAGTACGTCTTTTCAGGCCTACAACACGGCTCTTGACATCTCAGGGCGGGTAAGCCGTTTACGCGGCaacaccacaaagccacacatgcaa                                                                                                                                                                                                             >  2:101691/1‑215 (MQ=255)
                               tGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAATGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGCCTTATCAGGCCTACAAACCGGCCCTTTACCCCGCATGGCGGATAAGGCGTTTACGCCCCATCCGCCAAAAGGGcaaaaaccgaggccaa                                                                                                                                                                                                       >  2:387399/1‑223 (MQ=255)
                                       aTAAGCGTAAAGCGTATGAGCGTGGCCATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCAAAATGCCTGATGCGAAGCTAGTGCGTCTTATCAGGCCTACACACCGGCGCTTTAACGCGTCGGGCGGATAATGCGTTTACGCCGGATCCGACAATAGGGCCaagagggaacctgattcaaatag                                                                                                                                                                                              >  2:77121/1‑217 (MQ=255)
                                           gcgtaaagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGCTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACaaa                                                                                                                                                                                           >  1:463418/1‑237 (MQ=255)
                                             gaaaagcttaggggcggtgttATGGTGAAGGGGGGGGCGAACGTGACGATCATCGGGGAAAGGCCGGGGGTAAGGGGACCCGCCTTTAACCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATcc                                                                                                                                                                                         <  1:682170/218‑1 (MQ=255)
                                               aaagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACCCCGAATCCGCCAATATGGCCAAATGGGACCCCAATTCACCAAAAACCCCa                                                                                                                                                                                       >  2:916877/1‑238 (MQ=255)
                                                aagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCTTCCAAACCGGCCCTTTAACGCGTAGGGCGGTTAAGGCGTTTACCCCGACTCACCCCATAATGCCAAATGAGACGCCCATTCAACTCAAACCCaaa                                                                                                                                                                                     >  2:483096/1‑236 (MQ=255)
                                                     taTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTAAAACCCGGCTCTTTAACGCGTAGGGCGGTTCAGGCGTTTACCCCGCATCCGCCAATAATGCCAGATGGGACGCCAATCCCACTACAAACCCAGCCCCg                                                                                                                                                                                 >  2:284933/1‑238 (MQ=255)
                                                     taTGAGCGTGGCTATCGTGAAGCCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCCCCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTACCGCGTAGGGCGGCTAAGGGGTTTACCCCCCATCCGCCCCCAGTACCAGATACGACCCCCACTCAAATACAACCCCACCCCCg                                                                                                                                                                                 >  2:566754/1‑238 (MQ=255)
                                                       gggcggggtgttgggggaggggtGGCGCGCTGGTGAAGATCAGCGGGAAAAGGCCGGGTGTCTGGGACACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTcc                                                                                                                                                                              <  1:2906/217‑1 (MQ=255)
                                                                        aaGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATACGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata                                                                                                                                                             >  1:141089/1‑239 (MQ=255)
                                                                                     tCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCCACAACCAGACATTCAGCCCCACg                                                                                                                                                 >  1:420912/1‑238 (MQ=255)
                                                                                         gACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAAATGCGACGCCCATTCAACTACAATCCCAGCCCCCTCCCCCCCAAAAACCAGATATTCACCCCCCCAACCac                                                                                                                                            >  2:833389/1‑238 (MQ=255)
                                                                                               catcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCACCGCCGTCCCCACCAACCACCAAGTATTCAGCGCCACGACCAGcaccac                                                                                                                                      >  1:67011/1‑239 (MQ=255)
                                                                                                atcGCGGAAAAGGCCGGGGTCATGGGCCCCGCCATTAAACGCATCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGGGCGTCTTATCAGGCATACAAACCGGCTCTTTAAAGCGTAGGTCGGTTAAGGCGTTTACGCCGCATCCCCAAATAGTGCCAGAAGCGACACTCCTTCAACTACAATCCCACCGCCAGCCCCACACAAAACCAGATATTAGCGcccccgaccaaacccaca                                                                                                                                    >  2:665955/1‑223 (MQ=255)
                                                                                                 tcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCAACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTCCCATCCCACCGCCGTCCCCCCCAAAAACCAGAAATTCCACCCCACCACCAGcacccaaa                                                                                                                                    >  2:412690/1‑235 (MQ=255)
                                                                                                                 ggTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCCCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCCCGACCAGCACCACAAACACCCAAcccg                                                                                                                        >  2:25156/1‑233 (MQ=255)
                                                                                                                      aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCCCCCCGCCCAGCCCCAAAAACACCCACCCTGACTGttt                                                                                                                 >  2:472046/1‑238 (MQ=255)
                                                                                                                       tGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAGCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCACCGCCGTCCCCCCCAACAACCCGCTATTCAACGCCACGACCAGCCCCCAAACCacccaccccgctcgtttt                                                                                                                >  2:84247/1‑228 (MQ=255)
                                                                                                                       tGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGtttt                                                                                                                >  1:1033052/1‑238 (MQ=255)
                                                                                                                             ccGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGAACCAAAATCACCCAACCCGTCTGTTTTACACGtt                                                                                                         >  2:471775/1‑239 (MQ=255)
                                                                                                                              cGCCCTTAAACCCTGCCCCCCATTGGGCACAATGCCCTGTGGCGACGATGGGGTGTTTTACCGGGCCTCAAAACCGGCTCTTTAACGCGTGGGGCGGATAAGGCGTTTCCGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt                                                                                                         <  2:530312/239‑1 (MQ=255)
                                                                                                                              cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGCCGCTCATTCAACTACAATCCCAGCGCCGCCCCCACCAACACCCAGATATTCAgcg                                                                                                                                                      >  1:236127/1‑193 (MQ=255)
                                                                                                                              cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGTAATTCAGCCCCACGACCACCACCCCAACACCCCCCCCTGCCTGTTTTACGCGtt                                                                                                         >  2:933023/1‑238 (MQ=255)
                                                                                                                              cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCCCAATCCCCAAGCCCGCCTGTTTTACGCGctt                                                                                                        >  1:530312/1‑239 (MQ=255)
                                                                                                                              cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCCCCGTCCCCCCCAACAACCAGATATTCAGCGCCCCCCCCACCACCCCAATCACCCCCCCCTTCTTTTTTACGCGttt                                                                                                        >  2:553820/1‑239 (MQ=255)
                                                                                                                                 cattaaccggtcccGCCAATGGAACCAAATGCCTGATGCGACGTTAGTGGGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                                                                                     <  1:868457/227‑1 (MQ=255)
                                                                                                                                 aaTTAAACGCGTCCCCCAATGGGACAAAATGCCTGATGGGACGCGAGTGCGTCTTACCAGGCCTAAAAACCGGCTCTTTAACGCGTAGGGCGGAAAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                                                                                     <  1:42403/238‑1 (MQ=255)
                                                                                                                                    tAAACGGCTCCGCCAATGGAGCACAATGCCTGATGCGACGTTGGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGtt                                                                                                  <  1:843012/239‑1 (MQ=255)
                                                                                                                                            cccGCAAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTACCAGGCCTAAAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGAt                                                                                          <  1:25156/238‑1 (MQ=255)
                                                                                                                                             ccGCCAATGGAGCACAATGCCTGATGCGACGCTAGGGCGTCTTATCAGGCCTATAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGGTTACGCCGAATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGAt                                                                                          <  1:3342/238‑1 (MQ=255)
                                                                                                                                                  aaTGGAGCACAAGTCCTGGTGCGGCGGTAGGGGGTTTTATCAGGCCTACAAACCGGCTCTTTAACGGGTGGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCa                                                                                    <  1:277668/239‑1 (MQ=255)
                                                                                                                                                         cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACt                                                                              >  1:846392/1‑238 (MQ=255)
                                                                                                                                                           caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACtt                                                                             >  1:260223/1‑237 (MQ=255)
                                                                                                                                                                    gATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCAGTTTTACGCGTTTGCTGTTCACCAGACCGCCAATCAACTTGCTGTAACTg                                                                   >  1:387861/1‑239 (MQ=255)
                                                                                                                                                                     aTGCGACGCTAGTGCGTTTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACg                                                                                                                                                                                                           <  2:113842/102‑1 (MQ=255)
                                                                                                                                                                     aTGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACg                                                                                                                                                                                                           >  1:113842/1‑102 (MQ=255)
                                                                                                                                                                           cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCCCCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAAATCGCCCAACAACTTGCTGTAACTGGTGaaaa                                                            >  2:487868/1‑239 (MQ=255)
                                                                                                                                                                                     tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                                                                                                                           >  1:789448/1‑166 (MQ=255)
                                                                                                                                                                                     tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTTTTCCCCAGAACCCCCACCAACTTCCTGTCCCTGGTGAAAATcagaaggga                                                  >  2:669035/1‑236 (MQ=255)
                                                                                                                                                                                         catggccatACAAACCGGCTCTTTAACCGGTGGGGCGATAAAGGGGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAAcc                                              <  2:941859/231‑1 (MQ=255)
                                                                                                                                                                                               ccTACAAACCGGCTTTTTAACGGGTGGGGGGGAAAAGGCTTTTACGCCCCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAACCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGcc                                        <  2:1033052/239‑1 (MQ=255)
                                                                                                                                                                                                    aaaCCCGCCTTTTAACCGTAGGGGCGGATAAGGCTTTACCCCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGc                                   <  1:471775/239‑1 (MQ=255)
                                                                                                                                                                                                        cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCAAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGTAAATCAGCATCGGAACAAGAGCCAGAGCGAt                                >  1:595618/1‑238 (MQ=255)
                                                                                                                                                                                                            tttttAACGCGGAGGGCGGATAGGGCTTTTACGCCCCTTCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCa                            <  1:84247/236‑1 (MQ=255)
                                                                                                                                                                                                              tttAACCGGTAGGGGGGAAAAGCCGTTTACCCCCCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCaaaa                         <  1:472046/239‑1 (MQ=255)
                                                                                                                                                                                                                tAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTTAAAATCAGCAGTGGAACCAGCGCCAGAGCGTTACCacacct                       >  1:1069272/1‑234 (MQ=255)
                                                                                                                                                                                                                 tacccagtgggggggATAAGGCTTTTACCCCGCATCCGCCAATAGTGCCGAATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACt                       <  2:420912/230‑1 (MQ=255)
                                                                                                                                                                                                                   cgcgTAGGGCGGAAAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTaa                    <  1:833389/239‑1 (MQ=255)
                                                                                                                                                                                                                    ccgtgggggCGGTTAGGGCTTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCCCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTaa                    <  2:1069272/233‑1 (MQ=255)
                                                                                                                                                                                                                               gATATGCCGTTCACCCCGCTTCCTCCAAGTGGTCCAGATGCGACGCTCACTCACCTACAATCCCAGCGCCCGCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGa          <  1:323845/238‑1 (MQ=255)
                                                                                                                                                                                                                                aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACt        >  1:144849/1‑239 (MQ=255)
                                                                                                                                                                                                                                aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAAATTAACAATACCTGAc         >  1:120144/1‑238 (MQ=255)
                                                                                                                                                                                                                                  agggCTTTTACGCCCCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTGAACAGTACCTGACTCa      <  1:4587/237‑1 (MQ=255)
                                                                                                                                                                                                                                  aagggctttACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCa      <  1:933023/233‑1 (MQ=255)
                                                                                                                                                                                                                                  aaGGCTTTTACCCCGCATCCGCAAATAGTCCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCa      <  1:566754/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aaGGCCTTTCCCCCACATCCGCCAATAGTGCCAGATGCGACCCTCATTCAACTACAACCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCa      <  1:989271/239‑1 (MQ=255)
                                                                                                                                                                                                                                       gTTTACCCCGGATCCCCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGGCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAc  <  1:101691/238‑1 (MQ=255)
                                                                                                                                                                                                                                          tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                                                                                     >  1:904233/1‑104 (MQ=255)
                                                                                                                                                                                                                                          tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                                                                                     <  2:904233/104‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       
GTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAC  >  NC_000913/2511192‑2511657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: