Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 249,538 T→G 28.1% pseudogene (533/1713 nt) lfhA ← pseudogene, flagellar system protein, promoterless fragment, flagellar biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913249,5380TG28.1% 51.7 / 2.3 59pseudogene (533/1713 nt)lfhApseudogene, flagellar system protein, promoterless fragment, flagellar biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base G (16/5);  total (35/24)
Fisher's exact test for biased strand distribution p-value = 5.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGT  >  NC_000913/249305‑249772
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               
tGAGCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGggcggc                                                                                                                                                                                                                                           >  1:768603/1‑239 (MQ=255)
 gAGCGGGTTACCCTGTGCTTTGTCCCCCAGCGCCACCAGTTTGTACCCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGc                                                                                                                                                                                                                                           <  1:39038/238‑1 (MQ=255)
  aGCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCCTCCGCCCGCCGGTCAAACACAGCAGGGCGCTGAACAGCAAAAACCGCCGGGGCgggc                                                                                                                                                                                                                                           >  2:985931/1‑236 (MQ=255)
        ttACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGGCTTCCCgg                                                                                                                                                                                                                                 >  1:831184/1‑239 (MQ=255)
        ttACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCgg                                                                                                                                                                                                                                 >  1:920833/1‑239 (MQ=255)
          aCCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGTACAGCCAAAACGGCGGGTGCGGCACTCCCggg                                                                                                                                                                                                                                >  1:792529/1‑237 (MQ=255)
                tgCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCACGCAGGGCGCTGAACAGCAAAAACGGCCGGGGCGGCATTCCCGGAacccac                                                                                                                                                                                                                          >  1:482206/1‑235 (MQ=255)
                tgCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGCGGCATTCCCGGTaccacc                                                                                                                                                                                                                          >  1:241706/1‑238 (MQ=255)
                tgCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGCGGCATTCCCGGTaccacc                                                                                                                                                                                                                          >  1:47240/1‑238 (MQ=255)
                 gCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTCAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGATAGGCCATCCCGGTaccgccc                                                                                                                                                                                                                         >  1:850510/1‑237 (MQ=255)
                     tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCACCTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGGTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAAAGGCAGGGGGGGGAGTTCCCgggaacccccccaa                                                                                                                                                                                                                     >  2:434113/1‑227 (MQ=255)
                     tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCCGGACCACCGccccc                                                                                                                                                                                                                    >  1:558740/1‑236 (MQ=255)
                     tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGCGGCATTCCCGGTACCCccgcccgc                                                                                                                                                                                                                    >  1:186263/1‑239 (MQ=255)
                     tGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCCCGCTGGTTTCGGTGATGGGGCGGGTCCGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCGGCTCTCCGGTAAAACCAAACAGGGCGGCGCAAAGCCAAAAAGGCAGGGGGGGCCATTccggaagaccccgcag                                                                                                                                                                                                                       >  2:365419/1‑224 (MQ=255)
                      gTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCCGCGGCTGTTTGCTCATCCGCCCGCCGGTAAAACCCAGCAGGGCGCTGAACAGCAAAAACGGCCGGTGCGGCATTCCCCGTACCCCCGCCAGc                                                                                                                                                                                                                    >  1:525427/1‑238 (MQ=255)
                       ccccccgccccccccgttttGAACCCGGGCCTTAAACTTATGGGCCGGGTCGGCGGAATGTTTCCCCACTGGCCCGGCTCCTGGCGGGTTGCGGTGTTGGTGCGGGTCCGCGTTTCGGGGTTTTTCTCTCCCGCCCCCGCCGCAGGCGGCTTTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCAc                                                                                                                                                                                                                  <  2:768603/222‑1 (MQ=255)
                       cccccccccgcCACCAGTTGGTCACCGGGTCTTAAACTAGAAGGTCCGACCCGCGGAATGGTTTCCCAACTGCCCTGCTGCCCGCTGTTTCCGTGATGGTGCGGGTTCACGTTTCCGGGTCTTTTCCTTCCCCCCTCCGCCGCCGGGGGCTGTTTGCCCACCCGCCAGCCGGTAAAACCAGGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCAc                                                                                                                                                                                                                  <  2:831184/231‑1 (MQ=255)
                               cgcCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGCGGCCTTCCCGGGACCCCCGCCCGCACGAACATaa                                                                                                                                                                                                          >  1:974561/1‑239 (MQ=255)
                                 ccaccaGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCCGGGGCGGCAATCCCGGTACCACCCCCCCCACGaaacaaaa                                                                                                                                                                                                         >  2:386445/1‑232 (MQ=255)
                                 ccaccaGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGAGGCATTCCCGGTACCACCCCCAGccccgacaaaaa                                                                                                                                                                                                         >  1:711201/1‑227 (MQ=255)
                                     tttttgtaccccGGGGTTTAAACTGAAGGGGTCGTCGGGGGGATGTGTTCCCCACTTGCCCGGTGTTCGCGTGGTTTGGGGGGTGGGGCGGTCAGCGTTTCGGGGGTTTTTCTCTGCCGCCCCCGCCCCCGGGGGCTTTTTGCCCACCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCgg                                                                                                                                                                                                    <  2:773100/229‑1 (MQ=255)
                                          tggaaCCGGGGTTTAAGCTGATAGGCCCGATCCGGGGAAGTGTTTCCCAACTGACCTGCTGCCCGTTGTTTCCGGTGAGGGTGCGGGTCAGCTTTCCGAGGTTTTTCTCTCCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAACCCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCg                                                                                                                                                                                               <  1:434113/236‑1 (MQ=255)
                                          tGTACCCGGGGTTTAACTTTATGGGCTCGACCGGCGGAATGGTTCCCAACCTGCCCTGCTGCGCGGTGGTTTCGGTGTTGGGCGGGGTCGGGTTTTCGGGGCTTTTCTCTCCCGCCCCCGCCGCAGGCGGCTGTTGGCTCACCGGCCAGCCGGTAAACCCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCg                                                                                                                                                                                               <  2:844136/239‑1 (MQ=255)
                                                  aGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCCCCCGCCCGAACAAAATCCCGGGGGCGGTGTagg                                                                                                                                                                                        >  1:948666/1‑237 (MQ=255)
                                                  aGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGTGGGCATTTCCGGTACCACCGCCAGCACGAACATAATCCCGGTGCCGGGGTaga                                                                                                                                                                                        >  1:645092/1‑238 (MQ=255)
                                                  aGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGTCGGCATTCCCGGGACCCCCGCCAGCCCGAACACAATCCCgcgggcgggggtgg                                                                                                                                                                                        >  1:90041/1‑232 (MQ=255)
                                                              aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGGAGAGCACCGACGGGCt                                                                                                                                                                           >  1:631844/1‑239 (MQ=255)
                                                              aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGCGGCATTCCCGGGACCACCGCGCGCCCGAACACAATCCCggggtggggggcgggcacccacgg                                                                                                                                                                              >  1:319275/1‑214 (MQ=255)
                                                              aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGCGCGGCATTCCCGGTACCACCGCCAGCACGAACCTAATCCCGGGGGCGGCGCCGAGGACCGACGGGc                                                                                                                                                                            >  1:430293/1‑238 (MQ=255)
                                                              aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGAGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAACAACGGCCGGTTGGGGATTCCCGGTACCACCGCCCGCCCAAACCTAATCCCAGTGGCGGGGTAGGGCCACGCCGGGCt                                                                                                                                                                           >  1:774271/1‑238 (MQ=255)
                                                              aTAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTACTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCCGCCCGAACATAATCCCCGTGGCGGGGTAGGGGCCCCACggggt                                                                                                                                                                           >  1:575833/1‑236 (MQ=255)
                                                               tAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAAACCCGGTCGCCGGGGAGAGCACCGACCGGCt                                                                                                                                                                           >  1:790674/1‑238 (MQ=255)
                                                               tAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGTCGGCATACCCAGTACCCCCGCCATCCCGCAAATAAACCCGGGGGCGGGGGAGGGCACCGACgggtc                                                                                                                                                                           >  1:472299/1‑236 (MQ=255)
                                                                                 tGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCCCGAACAAAAACCCGGTGGCGGGGTAGAGCACCGACGGGCCTGCCAGCAGCCGGGTACGc                                                                                                                                                       >  1:340797/1‑239 (MQ=255)
                                                                                      ccccAACTCACCTGCTGCTCGCTGGTTGCGGTGATGGTGCGGGTCGGCTTTTCGAGGCTTTTCCCTCCCGCCTCCGCCGCCAGCGGCTGTTGGCTCACCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTc                                                                                                                                                  <  1:1006094/238‑1 (MQ=255)
                                                                                               gACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGGGCACCGACGGGCTTGCCAGCAACTGGTTACGCCCGGCATGGGCgg                                                                                                                                          >  1:523245/1‑238 (MQ=255)
                                                                                               gACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGGGGAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCCCGGCTTGGGCGa                                                                                                                                          >  1:273526/1‑239 (MQ=255)
                                                                                                                gTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCAGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGGGTAGAGCACCGACGGGCTTGCCAGCAGCTGGGTACGGCCGGCCTGGGCGGTATCGCCGTTGTc                                                                                                                             >  1:16282/1‑236 (MQ=255)
                                                                                                                          tGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGGGTAGAGCACCGACGGGCTTGCCCGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGGCGCggggg                                                                                                                >  1:721665/1‑237 (MQ=255)
                                                                                                                                          ctttccggggttttCCCTTCCCCCCCCCCCGCCCAGGGTTTGTTTGCCAACCCGCCAGCCGGTAAACCCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATcgccg                                                                                                   <  1:235539/229‑1 (MQ=255)
                                                                                                                                                      ttCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGGGCGGCATTCCCGGGACCACCGCCAGCACGAACATAATCCCGGGGGCGGGGGAGGGCACCGACGGGCTTGCCCGCAGCTGGTTAAGACCCGCCAGGGCCGAATCGCCGGTGTGGCTGACGCGGGGGACGAGAAAccccgcccggggggggag                                                                                      >  2:193042/1‑228 (MQ=255)
                                                                                                                                                      ttCTCTCCCCCCCCCCCCCCCGGGGGTTTTTTCCCCACCCGCCAGCGGGTAAAACCAAGCAGGGCGCTAAACAGCAAAAAGGGCAGGGGCGGCATTCCCGGTCCCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGagag                                                                                      <  2:340797/239‑1 (MQ=255)
                                                                                                                                                      ttCTCCCCCCCCCCCGCCCCCAGCGGTTTTTTGCTCATCCCCCAGCCGGTAAAACCAAGCAGGGCCCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGagag                                                                                      <  1:893698/239‑1 (MQ=255)
                                                                                                                                                                         gtcAGCGGCTGTTTGCGCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACGGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTg                                                                   <  1:210137/237‑1 (MQ=255)
                                                                                                                                                                         ccaagggggggTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTg                                                                   <  1:386445/229‑1 (MQ=255)
                                                                                                                                                                                             ccgccgACCGGGAAAACCAAGCAGGGCGCTGAAGAGAAAAACCGGCCGGTGCGGCAGTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGa                                               <  1:985931/239‑1 (MQ=255)
                                                                                                                                                                                              cGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGGGTAGAGAACCGCCGGGCTTGCCCGCAACCGGTTAAGCCCGCCATGGGGGGTATCGCCCTTTTCGCTGACGCCGGGGACGATAACGCCCACGCGGGGGGGGGGAGGCAGGGCAAGGATATGGGCCACCAGCCGgccgcccgc                                              >  2:267691/1‑236 (MQ=255)
                                                                                                                                                                                               gccggtgcgTAAAACACACCAGGGGGATGAACAAAAAAAACGGGAGGGGCGGCAACGCGGGCAGCTCCGCCAGCCCGGACATAATCCCGGTGGCGGTGTAGTGCAACGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATg                                             <  2:900290/231‑1 (MQ=255)
                                                                                                                                                                                                   cccGGTAAACCCAAGGAGGGGGCGAACCACAAAAAACGGGAGGGCGGGCATCCCCGGTCCCCCCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtca                                         <  2:16282/238‑1 (MQ=255)
                                                                                                                                                                                                    cgggaaaaaCAAAGGAGCGCGCTAAAACGAAAAAACGGGGGGTGCGGCTTTCCCGGTCCCCCCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtcat                                        <  2:948666/234‑1 (MQ=255)
                                                                                                                                                                                                    ccGGAAAAACCAGGCGGGGCTCTAAAACGAAAAATCGGCGGGGGCGGCTTCCCCGGTCCCCCCGCCAGCACGAACATAAGCCCGGTGGCGGTGTAGAGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGtcat                                        <  2:61135/239‑1 (MQ=255)
                                                                                                                                                                                                           acccgggCAGGGCGCTGACACGCAAAAAGGGCGGGTCCGGATTCCTCGTTACCACCGCCAGCACGACCATAATGCCGGTGGCGGGTTAGAACACTGACGGGCTTGCCAGCAGCGGGTTACGACCGTTATGGGCGATATCGGCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGcaca                                 <  2:649693/233‑1 (MQ=255)
                                                                                                                                                                                                           acccAAGCAGGGCGCAGAACAGCAAACACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGcaca                                 <  1:192823/237‑1 (MQ=255)
                                                                                                                                                                                                           aaCCAAGCAGGGCCATGAACAGGAAAAGCGGCAGGTGCGGCCTTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGcaca                                 <  1:23376/239‑1 (MQ=255)
                                                                                                                                                                                                                     ggCGCTGAACAGCAAAAACGGCAGGGGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGAGTAGAGCACCGACGGGCCTGCCCGCAGCTGGTTACGCCCGGCCAGGGCGCAATCGCCGGTGTCGCGTACGCGGGGAGCGAGAAATCCCGCCACGGGGGAGGGAAGCAGGGAAGGGAGTTGGGCCCCCCGGCCGTCGCCGGTggggacaagcacaaaccaccggg                       >  2:348583/1‑218 (MQ=255)
                                                                                                                                                                                                                       cgcTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAg                     >  1:878615/1‑239 (MQ=255)
                                                                                                                                                                                                                                   aaaaCGGCCGCGTCGGCATTCCCGTTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGgcg         <  1:725209/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aagggCAGGTGCGGCATCCCCGTTCCCCCCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCt       <  1:793886/236‑1 (MQ=255)
                                                                                                                                                                                                                                          cgggTGCGGTCTCCCCGGTCCCCCCCCCAGCCATGACCTAATCCCGGTGGCGGTGGAGGGAACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGt  <  2:523245/237‑1 (MQ=255)
                                                                                                                                                                                                                                           gggTGGGGCTTTCCGGGCCCCACCGCCAGCCCGAACATAATCCCGGTGGCGGTGTAGGGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGt  <  2:430293/237‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               
TGAGCGGGTTACCCTGTGCTTTGTCCACCAGCGCCACCAGTTTGTAACCGAGGCTTAAACTGATAGGCTCGATCAGCGGAATGGTTTCCCAACTGACCTGCTGCTCGCTGGTTTCGGTGATGGTGCGGGTCAGCGTTTCGAGGCTTTTCTCTTCCGCCTCCGCCGCCAGCGGCTGTTTGCTCATCCGCCAGCCGGTAAAACCAAGCAGGGCGCTGAACAGCAAAAACGGCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGATAATCGCCGCCGCGGTGGAGAGCAGCAGGGAAGGGATCTGCGCCACCAGGCCGTCGCCGATGGTCATCAGCACATACTGCTGGAAGGCAGCATCGGCGCTCAGGT  >  NC_000913/249305‑249772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: