Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,828,504 | T→G | 29.5% | V257G (GTG→GGG) | srlD → | sorbitol‑6‑phosphate dehydrogenase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,828,504 | 0 | T | G | 29.5% | 110.1 / 9.4 | 61 | V257G (GTG→GGG) | srlD | sorbitol‑6‑phosphate dehydrogenase |
Reads supporting (aligned to +/- strand): ref base T (5/35); new base G (19/2); total (24/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.36e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGG > NC_000913/2828267‑2828736 | cGGTGCATTGCTTGATGCCCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGGTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg < 1:1088462/239‑1 (MQ=255) cGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg < 1:302746/239‑1 (MQ=255) caggCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg < 1:795764/235‑1 (MQ=255) tGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑Tg > 1:697396/1‑239 (MQ=255) gtccttCCCTGGTGCCCGGTAACCTTCTTAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑Tg < 2:341942/235‑1 (MQ=255) tcctcggtccTCGTTAACCTGCTGAATTCGCCGATGTTCCCGTCTCTGTTGCCAAAATACGCGACCAAGCGGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCATTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg < 2:510913/230‑1 (MQ=255) tccctggttgccGGTAACCGTGTGAAATCGCCGATTTTCCAGTCACTGTTCCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg < 2:104982/228‑1 (MQ=255) gATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGGCCTAAGGCGTCGTACTGCACCGGACAGTCGTTCAATGTCACCGGCGGTCAGGTggagttttgtgcca > 1:1031900/1‑226 (MQ=255) tGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGCCAGTCGGTCAATGTCCCCGGCGGGCCGGGGG‑TGTTcccggtcttt > 2:495493/1‑230 (MQ=255) tGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑AGTTTTgtttcaaa > 1:183503/1‑232 (MQ=255) gCTCGGTAACCCGCTGAAATCGCCGAGTTTCCATGCACCGTTGCCACAATACGCGACCAAGCGTGGGATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGGCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑Ag < 2:860409/239‑1 (MQ=255) gttcccTGCTTAATTCCCCGTTTTTCCCGTCCCTTTTGCCAAAATACGCGCACAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGgag < 2:960795/235‑1 (MQ=255) aaCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGAACAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGgaga < 1:760324/238‑1 (MQ=255) aCCTGCTGAAATCGCCTGTGTTCCAGTCACTGTTGCCACAATCCGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATc < 1:158911/239‑1 (MQ=255) gctgAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTCCGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGGCAGGGGG‑TGGTTTGTTCAAA‑Agacgggagaacat > 2:1002788/1‑227 (MQ=255) gctgAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATTTCACCGGCGGGCCGGGGA‑TGtttttggcaaaaacggggaaccaaa > 2:379413/1‑216 (MQ=255) tgAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGG‑TGTTCTGATCACA‑CGCGGAGATACCTTaa > 1:974916/1‑239 (MQ=255) aTCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGTTTAAC‑AGCGGGGATCCATTAAggg > 1:624529/1‑237 (MQ=255) aTCGCCGATGTTCCAGTCACTGTGACCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGAt < 1:658352/239‑1 (MQ=255) cGATGTTCCAGTCACTTGTGACACAATACGCGACCAAGCTGAGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGGCAAAGTACCGCTCAAACGCGGCTGCGATTAACAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCg < 1:1048275/239‑1 (MQ=255) cGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCg < 1:8225/239‑1 (MQ=255) aTGTTACAGTCCCTGTTGCCCCAATACGGGACCAAGCTGGGTAGCAAACCGGAGCAAGTCCATCAGGATTACTACGACAAAGTCCCGCTCAACAGGGGCTGCGATTAGCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTg < 2:355651/239‑1 (MQ=255) ttttccctttaCTTTTCCCACAATTCGCCACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTga < 2:715235/229‑1 (MQ=255) gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTga < 1:591252/238‑1 (MQ=255) gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTga < 1:768403/238‑1 (MQ=255) tcccggtccTGTTGCCCCAATACGGGACCAAGCTGGGTTCCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTACCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGAc < 2:753790/231‑1 (MQ=255) tCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATTAAC‑AGCGGGGGTCCCTTAAGGATTTCCCTGAGACtataa > 1:694925/1‑238 (MQ=255) aCTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGG‑TGTTTTTGTTACC‑AGCGGAGATCCATTAATGATCTCCGTGCGACtcacaaa > 1:1031134/1‑233 (MQ=255) tGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg < 1:665977/193‑1 (MQ=255) tGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGCCAAGGGAGAGTTTTGTTCAAC‑CACGGCATCCCATAAAGGATCCCCGCgagatataaaaa > 2:473243/1‑230 (MQ=255) gTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTTTGGTCAAC‑CACGGCGAACCCTTAAGGATATCCCTgggagcatacgata > 1:753790/1‑228 (MQ=255) ccgACCAAGGTTGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg < 2:183503/238‑1 (MQ=255) cAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcat < 1:379413/239‑1 (MQ=255) tGGGTTTAAACCCGGATTAAGTCGAGCCGTATTAACTCGACAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg < 2:1031900/237‑1 (MQ=255) tGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg < 1:252183/237‑1 (MQ=255) tggTATTAAACCGGATCAAGTCGAGCAGTATTGCAGCGAAAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAgg < 2:974916/236‑1 (MQ=255) ggTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCTTTAAGGCTTTCCGTGAGACTATAAGATGCCCGATgcgcgccccgcatcctgcat > 1:173333/1‑223 (MQ=255) gATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGGTCTGATCAAA‑AGCGGAGATCCATTAAGGATCTCCGGGGGACTATAGAATGCCTGATGCGCCACCCCCATCCGGCCTACAGGGCTTcc > 1:342189/1‑238 (MQ=255) gTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGA‑TGTTTTTGTCAAC‑AGAGGAGATACATTAAGGATCTCCGCGAGACCATAAAATACCCGATGCGCCACGCCCATCAGGCCAACAGGACCTCccccccac > 2:687640/1‑235 (MQ=255) gCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGG‑TGTTCTGATCAAC‑AGCGGAGAGCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACCTTa > 1:305637/1‑239 (MQ=255) gTATTACATCGACAAAGGCCCGCACAAACGGGGGTGCGATTAGAAAGGTGGGCGGAATAGGGTGGTGTTCTACCCCAGTCCAAAGGGGTCGTACTGCCCGGGAAAGTCGATCAATGTCCCGGGCGGTCAGGTGA‑TGTTCTGAAAAAC‑AGCGGAGATCCATTAAGGATCTCCGGGAGCCTATAGAATGCCTGATGCGCTACGCTCATCGGGCATACAGGACTTCCGCCACTACATTAAgg < 2:342189/239‑1 (MQ=255) tATTACATCGACAAAGTCCCGCTAAAACGGGGCTGCGATTAACAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCCCCGGACAGTCGATCAATGTCACCGGGGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCa < 1:249435/227‑1 (MQ=255) aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGG‑TGTTTTGCTCAACAACCGGGGACCCTTTAGGGTACCCCG‑GGGAATACAGAATGCCCGTTGCGCTACGCCCATCCGGCATACCAGGCCTCCGCCCCCACATTAAgatactctcttat > 2:249435/1‑227 (MQ=255) aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGG‑TGTTCTGCTCAAC‑AGCGGAGAACCCTTAAGGATGTCCGTGCGACTATAGAATGCCCGATGTCGTGCGCGCATCGAGCCTACAAGACCTCCGCCAccaacaaaagggaaaaaaaaag > 2:1009547/1‑218 (MQ=255) ccGCTCAACCGCGGCTGCTATTATCAAGATGTGCTGAATCTGCTGCTGTTCTACGCCAGTCTAAAGGCGTCGTCCTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATAAAC‑AGGGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCTTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCa < 1:304565/239‑1 (MQ=255) cgcatttATCAAGGTGTGCTGAATTGGCTGCTGTTCAACGCCAGTCCTAAGGCGTCGTACGGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATAAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGc < 1:687640/233‑1 (MQ=255) gtttcaagtaTGGGCTGATTGTGCTGTTGTTCTCCGCCAGCCCAAAGGCGTCCTACGGCCGCGGACGGTCCAACATGCGCACCGGCGGCAGGGTGA‑TGTTCTGATCACC‑AGGGGAGACCCTTAAAGGAGCTCGGGGAGACTAAAGAATGCCTGTGGCGCTCCGCTCATCCGGCATACAGGCTTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt < 2:697396/229‑1 (MQ=255) aTTATCAAGATGTGCTGAATATGCTGCTGTTCTCCGCCAGCCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCCCTCCGCTCATCAGGCATCCAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt < 1:604932/238‑1 (MQ=255) tCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTg < 1:803263/132‑1 (MQ=255) gCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGAA‑TGTTCTGATCAAC‑AGCGGGGATCCATTAAAGGTCTCCCGGAGAATATAGAATGCCTGATGGGCCAAGCTCACCCGGCATAACGGGCTTCCGCCCCCACATTAAGGAAAAGATATGGTGTTCGCCCTCaccaccccccccggttaatccccgggggg > 2:329255/1‑216 (MQ=255) aaTTTGCTGCTGTTCTACCCCAGCCCTAAGGCGTCGTACGGCACCGGACAGTCGATCAATGCCCCCGGCGGTCAGGTAA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCaa < 1:329255/239‑1 (MQ=255) attttgtgttgTTCTACGCCAGTCTGAGGGGGTCGGCCTGCGCCGGACAGTCGATAAATGTCACCGGGGGTCAGGTGA‑TGTTCGGATCAAC‑AGGGGAGATCCATTAAGGATCTCCGGGAGACTATAGAATGCCTGATGCGCTACGTGCATCGGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAAc < 2:412961/230‑1 (MQ=255) aGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAAC‑AGCGGGGATCCATTAAGGATCTCCGTGAGACTAAAGAATGCCTGAAGCGCCACGCCCATCCAGCCTAACAGACTTCCCCCACCACCTTAATGAAAAGATATGGGATCCCCACTCATCACCCCCCCCGCTATCGCCCGGTGGG‑GGCAACCGGCCCTAAGGCGCG‑GGGCA‑AAtt > 2:365922/1‑236 (MQ=255) cACCGGACAGTCGATAAATGTCACGGGCGGTAGGGTGA‑TGTGCTGATAACC‑GGCGGAGATCAATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGc < 1:365922/239‑1 (MQ=255) acgggACAGTGGATCAATGCCCCGGGCGGTCAGGGGA‑TGTTCTGACCAAC‑GGCGGAGATCCATTAAGGATCGCGGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCGGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGc < 1:1002788/235‑1 (MQ=255) gACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCCTACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGCCGCCGTTATCGCCCTGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTTTCGTTTTAACCGTGCCTTc > 1:331446/1‑238 (MQ=255) gACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAAC‑AGGGGGGATCCATTAAGGATCTCCGTGGGACTATAGAATACCTGATTCGCCACGCCCATCAGGCATACAGGACTTCCGCCACTACACTAAGGAAAAGTTATGGTATCCCCACTCATCACCGCCCCCGCTATCTCCCGGTTGT‑CGCCACTGG‑CCCTTGGCGGC‑CGGGA‑AAATTTTCGTTTTAACCATgtcctcc > 2:1064919/1‑233 (MQ=255) cgacaaaTGGCCCCGGGGGTCGGGTGA‑TGTTCTGATCAAA‑GGGGGAGATCCATTAAGGCTCTCCGTGAGCCTATAGAATGCCTGATGCGCTACGTTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGacaca < 1:871601/233‑1 (MQ=255) aTCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGGTCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGGGACTATAAAATGCCCGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGGTATGGGATCCGCACTCATCACCCTCGCCGTTATCTCCTGGTGTG‑GGAGACCAG‑CCTCAGGGGGC‑GGGCACAATTTTTCTTTTAAACCG‑GCCTTCGccacccaa > 2:940853/1‑235 (MQ=255) cAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAAGGGCTCCCGGAGACCATAGAATGCCTGATGCGCTACCCCCATCAGGCATACAGGACTTCCCCCACTCCATTAAGGGAAAGTTATGGTATCCGCACTCATCAACGGCCCCGTTATTGCCTGG‑GTGTTGCAACTGG‑CCCTAGGCGGCGTGGCA‑AATTT‑TCTTTTTTACCCGGCCCTCCccacaccca > 2:871601/1‑235 (MQ=255) tCAGGGGA‑TGTTCGGACCAAC‑GGCGGGGATCCATTAAGGATCCCCGGGAGACTAAAGAAGGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCggg < 1:940853/238‑1 (MQ=255) | CGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAAC‑AGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTG‑CGCAACTGG‑CCTTAGGCGGC‑TGGCA‑AATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGG > NC_000913/2828267‑2828736 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |