Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,931,393 T→G 20.1% V114G (GTG→GGG)  ygdG → Ssb‑binding protein, misidentified as ExoIX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,931,3930TG20.1% 133.7 / 3.2 66V114G (GTG→GGG) ygdGSsb‑binding protein, misidentified as ExoIX
Reads supporting (aligned to +/- strand):  ref base T (20/30);  new base G (13/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 2.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTATGCACAGCCAGCCAACCCACGCGGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAA  >  NC_000913/2931161‑2931615
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
ttatggCACCGCCACCACACCCAGGCGGTCGCCGTTTTTGAGGAAAAAACCCGAGGTAGCGGCGGGGTTAACAAGCGTTTACCAGATTAAAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGaca                                                                                                                                                                                                                              <  1:529392/232‑1 (MQ=255)
  tATGCACAGCCAGCCAACCCACGCGGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGGGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCAATGACTTAGCCGCCACGCTGGCGGTCAAAGGGAAACA‑g                                                                                                                                                                                                                          >  1:811932/1‑238 (MQ=255)
   tgccacgccctgcgaccccACGGGTTCGCCGTTTTGGATGATGAAAACCGCAGTGTGGCCTGGGGTCAGCAGCGTTTACAAAATTCCAAA‑GGGGGCCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGc                                                                                                                                                                                                                        <  1:1056176/223‑1 (MQ=255)
         ggccacgcaACCCACGCGGTCGCCGTTTTTGATGATGAAACCCGCAGTAGGGGCTGGCTTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCa                                                                                                                                                                                                                  <  1:295674/232‑1 (MQ=255)
          gccagccaACCCACGCGGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCCAAGGGA‑ACACGGCCGGGcct                                                                                                                                                                                                                 >  1:387700/1‑237 (MQ=255)
           ccagccaACCCACGCGGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGGGACACA‑GGCCggcgtca                                                                                                                                                                                                                >  1:740504/1‑234 (MQ=255)
            agcccaccccagcgggccGCCGTTTTGAGAGAGGAAACCCGCAGGAGGGGCGGGGGTACTCGGGTTTTACCAGATTACAAG‑GGGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCAt                                                                                                                                                                                                                 <  1:343597/220‑1 (MQ=255)
               ccaCCCCACGGGGTCGCGTTTTTTGATTATGAAACCCGCAGTAGGGGCTGGCGTCATCAGGTTTTCCCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGc                                                                                                                                                                                                            <  1:547050/239‑1 (MQ=255)
                       cgcgGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCCCGCTGGCGGTCAAAGGGACACA‑GGCCGGGCACCAGGGCACGATTg                                                                                                                                                                                                    >  1:164455/1‑239 (MQ=255)
                        gcgGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGCCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACTCTGGCGGCCCAAGTGACACC‑GGCCCGGCATCCGGCAACCATTGt                                                                                                                                                                                                   >  1:408018/1‑239 (MQ=255)
                             cGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCCAGGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGAGGACTGACCCGCCACGCCTGCGGGCAAGAGGACACA‑GCCCGGGCACCAGCCaaaagaagaattgc                                                                                                                                                                                              >  2:897372/1‑227 (MQ=255)
                              tgggaattgtgtaaaaggacacacAGGAGGGGCGGGCGTCAACAGGTGTTCCCAGTTTAAAAG‑GCGGGCCGCCCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGa                                                                                                                                                                                              <  1:467465/215‑1 (MQ=255)
                              gCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCCAAGGGACACA‑GGCCGGGCTCCTGGCAACGATTATTTCgcc                                                                                                                                                                                             >  1:128309/1‑237 (MQ=255)
                                    tttGGTGATAAAACCCGCGGTGGGGGTTGGGGTCACCGGGGTTTACCAGATGAAAAG‑GCGGGGCGCCCGCCAATGCCGGAAGGGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATaa                                                                                                                                                                                       <  2:408018/239‑1 (MQ=255)
                                          gatgaAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAAGGACACA‑GGCCGGGGACCAGGCAACAGTTGTTTCGACAGAGAAAgcgc                                                                                                                                                                                  >  1:832686/1‑235 (MQ=255)
                                                  ccGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGGCAACCAGCGGCAACGAAGCCGATGACTTAACCGCCACGCTGGCGGTCAAAGGTACACA‑GGCCGGGGATCAAGCAAACATTGTTTCggaaataaaaagacaacggccag                                                                                                                                                                         >  2:223838/1‑217 (MQ=255)
                                                                ggCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGGCAAAGTGACACC‑GGGCGGGCATCCGGCAACGATTTTTTCGACCGAaaaaag                                                                                                                                                                                    >  2:467465/1‑212 (MQ=255)
                                                                     aagcaGCGTTTACCAGATTACAAG‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACg                                                                                                                                                      <  1:177745/236‑1 (MQ=255)
                                                                      cccccgcttttCCAGATTCCAAAGGGGGGTCGCCCCGCAAGCCGGGAAGGGTTGCCCGCCGGGATGCCTGCATTACGGCCCCCCTTTGGGCAACGGGGCGTCCCGGGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACg                                                                                                                                                      <  2:1072316/232‑1 (MQ=255)
                                                                                ccaaGATTACAAA‑GCGGGTCGACCGCCAAGGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGAtt                                                                                                                                           <  1:767164/236‑1 (MQ=255)
                                                                                     aTTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGACGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCAACAGGCCACCATTGTTTCGCCAGGAAAAGGCTACTGTCAGTTCATTTTCCCCCCATTTCGTTTTTGTGAtttttt                                                                                                                                       >  2:157105/1‑234 (MQ=255)
                                                                                     aTTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGGGAAACA‑GGCCGGGCATCAGGCAACGATTGTTTCGTCAGATAAAGCCTACGTTCGGTTAATTTCACCGACCTTACCTATTCCTGATTACTTc                                                                                                                                      >  1:14509/1‑239 (MQ=255)
                                                                                          aaa‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGGTACTTTCACCGACATTACGGATTCGTGATTACTTccccaa                                                                                                                                 >  1:696204/1‑235 (MQ=255)
                                                                                            a‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCCTCACGCAACGATTGTTTCGACAGATAAAGGCTCATGGCAGATTCTTTCCCCCCCATTACGGTTTTGCGATTAAtttccaaaaa                                                                                                                               >  2:1028384/1‑231 (MQ=255)
                                                                                              gggggTAGACCCGCAATGCCGGAAGGGTTGCACGACGGGATGCCTGCATTACGCGCCGCCTTGGGGCAAGGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑a                                                                                                                                 <  1:567491/233‑1 (MQ=255)
                                                                                                  gTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGGGACACA‑GGCCGGGCATCCGGCAACGATTGTTTCGGCAGAAAAAGGGTACTGTCAGGTACTTTCACCGCCATTACGTATTCGTGATTTATTCCAA‑AAAAATTgg                                                                                                                         >  2:221441/1‑239 (MQ=255)
                                                                                                  gTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGGGACACA‑AGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAAGCCACTGTCAGTTACTTTCACCGACCTTACTGATTTGTGATTATTTCCAG‑AAACGTTgg                                                                                                                         >  2:36322/1‑239 (MQ=255)
                                                                                                         gCCAATGCCGGAAGGGTGCCCCGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATg                                                                                                                  <  1:36322/239‑1 (MQ=255)
                                                                                                         cccaagtccGGAAGGTTTGCCCGACGAGATGCCTTGATTCCGGGCCCCCTGTGAGCCAGGCGGCGTCCCGGTCTGGTCAACCCGGGGAAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGGGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATg                                                                                                                  <  2:655415/232‑1 (MQ=255)
                                                                                                                    aaGCGTTGCACGACGAGATGCCTGCATTCCGCGCCGCCTTTGAGCAACGCGGCGTCCCGGGCTGGTCAACCAGCGGAAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTAt                                                                                                        <  1:223838/238‑1 (MQ=255)
                                                                                                                    aaGAGTTGCCCGACGAGATGCGTGCATACCGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCACCCAGGGGAAAGGAAGCCGATGACTTGGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTAt                                                                                                        <  2:169854/238‑1 (MQ=255)
                                                                                                                      gagTTGCACGGCGAAATGCCTGCATTACGGGCCGACTTTGGGCAACGGGGCGTCCCGTGTCGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCg                                                                                                      <  1:754635/238‑1 (MQ=255)
                                                                                                                                     gtGCCTGCATTACGGCCCCCCTTTGACCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCg                                                                                       <  1:424555/237‑1 (MQ=255)
                                                                                                                                            aaaagaggcgggccTTTGGGCACCGGGGCGCCCCGGGGTGGCCAACCGGGGGAAAGGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCg                                                                               <  2:103397/228‑1 (MQ=255)
                                                                                                                                             aTTCCGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCg                                                                               <  1:221441/238‑1 (MQ=255)
                                                                                                                                              ttACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCCAAGTGACCCA‑GGCCGGGCCTCAGGCAACGATTGGTTCGGCAGATAAAGGCCACCTTCAGTTTCCTTTCCCCACATTACCTATTTGGGATTACTTCCCGAAAACGTTGCCGGCAAG‑GCCATTTATCGTAAAAAAATTTGGGGGTCAACCcca                                                                             >  2:291230/1‑239 (MQ=255)
                                                                                                                                                aCGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCCACGCTTGTTTCGACAGAAAAAGGTCACTGGCAGTTACTTTCACCGCCATTACGTATTTGTGGTTATTTCCCG‑AAACTATGGCTTGATTCGCCCTTTTTTCAAAAAAAAATTTGCGTTCAACcgccgc                                                                           >  2:807140/1‑239 (MQ=255)
                                                                                                                                                 cgcgCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGGCAAAGGTACCCA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAAGCAACTGGCAGTTACATTCGCCTACGTTACGTATACGTGATTACATCCAG‑AAAAATTGGCTGGATGGGACCTTTTTCAGTAAAAAAtttttgcgtaaaacgccgca                                                                          >  2:360366/1‑222 (MQ=255)
                                                                                                                                                   cgccggcagtGAGCAACCGGGCCTCCCGTGCTGGTAAACCAGCGGCAACGAACCCGATGCTTTAGCCGCCTCGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCCGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCTCCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGt                                                                        <  1:241463/230‑1 (MQ=255)
                                                                                                                                                      cgccTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGCATTCGTGATTACTTCCAC‑AAACGTTGGCTGGATGCGCCCTTTATCGCTAAAGAATTTGACGTTCAACCGCAACAGTTg                                                                      >  1:90609/1‑238 (MQ=255)
                                                                                                                                                      cgccTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGAGACACA‑GGCCGGGCATAAGGCCACGATTGGTTCGACAGGTAAAGGCTACTCTCAGTTCCTTTCCCCGCCCTTACGGATTCCTGATTACTTCCCG‑AAACACGGCTTGGGTGAGCCCTTTATCCTACAACAATTTGGGGTTCAAcccacaacagcg                                                                      >  2:3052/1‑228 (MQ=255)
                                                                                                                                                        ccTTTGGGCACCGCGGCCCCCCGGGTGGGTCACCCGGGGGCAACGAGCCCGATGCCTAGGCCGCCCCGCTGGCGGTAAAAGTGACCCA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGccc                                                                   <  2:372728/239‑1 (MQ=255)
                                                                                                                                                                  aCGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGCCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCCTTTATCGATAAAGAATTTGGCGTTCAACCCCCGCAGGTGc                                                                     >  1:103397/1‑227 (MQ=255)
                                                                                                                                                                   cgcgGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCCAAGTGACACA‑GGCCGGGCATCAGGCAACGCTTGTTTCGGCAGATAAAGGCTACTGCCAGTTCCTTTCACCGCCATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCCTCTTTCGTTAAAGAATTTGGCGGTCAACCCCCCCAATTGCCCGATTAcgtggg                                                        >  2:836846/1‑234 (MQ=255)
                                                                                                                                                                   cgcgGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGGATTCGTGATTACTTCCAG‑AAAAGTTGGCTGGATGCGCCATTTATCGATAAAAAATTTGGGGTTCAACCCCAGCAAGTTCCCCAATAATgggg                                                        >  2:305312/1‑239 (MQ=255)
                                                                                                                                                                   cgcgGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGGGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTTTCGGTAAAGAATTTGGCGTTCAACCGCAGCCGTTGCCCGATTACTgggg                                                        >  1:191239/1‑239 (MQ=255)
                                                                                                                                                                        cgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGCCATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGGTTACTGGGGACttt                                                   >  1:275370/1‑238 (MQ=255)
                                                                                                                                                                           cccGGGGTGGTCAACCAGGGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCg                                                 <  1:291230/238‑1 (MQ=255)
                                                                                                                                                                           cccGGGCTGGCCACCCAGCGGCAACGAAGCCGATGACTTAGACGCCACGTTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGGAACGGTTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTATTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCg                                                 <  1:980457/238‑1 (MQ=255)
                                                                                                                                                                             cGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCgggg                                              <  1:132577/239‑1 (MQ=255)
                                                                                                                                                                                gcgggTCAACAGGGGGCAACGAAGCCGATGGCTTAGCCGCCCCGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGAt                                            <  1:424550/235‑1 (MQ=255)
                                                                                                                                                                                  tGGTCACCCGGCGGAACCGACGCCGATGAGTTCGCCGCCACGCTGGCGGTCAAAGTAACACC‑GGCCGGGCATCAGGCAACGATTGTTTCTACAGATAAAGGCTCCTGTCAGTTTCTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATcag                                         <  2:811660/239‑1 (MQ=255)
                                                                                                                                                                                  gggTCACCCAGCGGCAACGAAGCCGATGACTTGGCCGCCACGGTGGCGGTAAAAGTGACAAA‑GGCCGGGCATCAGGCAACGATTGTTGCGACAGATAAAGGCTACGGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATcag                                         <  2:191239/238‑1 (MQ=255)
                                                                                                                                                                                   gggcAACCAGGGGCAACGAAGCCGATGCTTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATcag                                         <  1:1028384/235‑1 (MQ=255)
                                                                                                                                                                                    gTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCACCGCATGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGGATTTGTGATTCCTTCCAG‑AAACATTGGCTGGATTCGCCCTTTATTGGTAAAAAATTTTGCGTTCAACCGCAGCAGTTTCCCGATTACAGGGGAGTGGGGGGGacgagaa                                       >  2:132577/1‑233 (MQ=255)
                                                                                                                                                                                    gTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCCTTTTTCGCTTAAAAATTTGGCGTTCAAACGCAGCAGTTGCCCCGTTAATGGGGACTTTCGGGGGTcagcc                                       >  2:659588/1‑238 (MQ=255)
                                                                                                                                                                                         ccAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTggggctggctcttttacacatctccg                                  >  1:70366/1‑217 (MQ=255)
                                                                                                                                                                                              ggCAACGAAGCCGATGCCTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCaaa                                <  1:897372/236‑1 (MQ=255)
                                                                                                                                                                                              ggCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGt                             >  1:180838/1‑239 (MQ=255)
                                                                                                                                                                                              ggCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGGCAGTTACTTTCACCGACATTACGAATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGGTTACTGGGGCTTTGCGGGGATCAGCAGGTCAAAggg                             >  1:849253/1‑238 (MQ=255)
                                                                                                                                                                                                        cgggtGACTTAGCCGCCCGGCTGGCGGTAAAAGGGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGAAAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGgtg                     <  2:275370/233‑1 (MQ=255)
                                                                                                                                                                                                                ttAGCCCCCATGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTg                                                   <  1:985720/199‑1 (MQ=255)
                                                                                                                                                                                                                 tAGCCCCCCCGCTGGGGGTAAAAGTGACCCA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTTCTGTCAGTTTCTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATc          <  2:180838/239‑1 (MQ=255)
                                                                                                                                                                                                                        cACGCTGGCGGTCAAAGTGACACA‑CGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGGTACTTTCAACCGCATTACGCATTCGTGATTACTTCCAG‑AAACGTTGGCTGGGTGCGCCATTTATCGATAAAAAATTTGGCGATCAAGCGCAGCAGTTGCCCCATTACTGGGGACTTGCGGGGATCACAAGTTACAAGGTACCGGGGGTTTCGGGaaagtgcccaa   >  2:948834/1‑230 (MQ=255)
                                                                                                                                                                                                                         aCGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGGAACGATTGTTTCGACAGATAAAAGCTACTGTCAGTTACTTTCACCGACATTACCTATTCGTGATTACTTCCCG‑AAAAGTTGGCTGGCTGCGCCCTTTTTCGATAAAGAATTTTGCGTTCAACCGCAGCAGTTGCCCGGTTACTTGGGACTTGCAGGGGGCAGCGGGTCAAAGGGAACGGGGGTTGGGGGAAAAGGACaaaa  >  2:532852/1‑239 (MQ=255)
                                                                                                                                                                                                                         aCGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAAGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGGTTACTGGGGACTTTCGGGGGTCAGAAGTTTAAAGGGACCGGGGGGTTGGGGAAACGGagaaaa  >  2:709782/1‑234 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
ATTATGCACAGCCAGCCAACCCACGCGGTCGCCGTTTTTGATGATGAAAACCGCAGTAGCGGCTGGCGTCATCAGCGTTTACCAGATTACAAA‑GCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACA‑GGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAG‑AAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAA  >  NC_000913/2931161‑2931615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑