Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,793 A→C 18.3% V454G (GTG→GGG)  speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,7930AC18.3% 108.8 / 4.0 66V454G (GTG→GGG) speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (25/25);  new base C (3/12);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 7.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGA  >  NC_000913/3107555‑3108006
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                         
aaaCAGAAGGGGGTTGGGATGCGCTTTCTTCGGGGCAATGCCGTTCTGCCGGCGGTGGTGCGCCAGAATCGGCGGCGGAAGGCCAAAGTCGCTATATTCGCCGGGTTCGGCATCGATACCTGGTGTAGTGAGTAACTGCTTGCACGGATCAACAAAATACTGATCCGCGGCATGTCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAaca                                                                                                                                                                                                                           <  2:79985/239‑1 (MQ=255)
      aaTGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAacaccgga                                                                                                                                                                                                                      >  1:766398/1‑234 (MQ=255)
                  aTCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGCCGGTCGCTGGGTAACACTGATGTGGGGTAATccc                                                                                                                                                                                                         >  1:397981/1‑238 (MQ=255)
                     cccccctcccgggcaaagctccGTCCCCACGAAGAAAGGCCCCCAGACTCGTCGCCGGAACGCAAAAGTCGCTATATTCGCCGGTTTCGGCATAGATACCGGGTGTAGTGAGTACCAGCTGGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTTCCACTTCGCCCCCGGCTCAAAACGGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGc                                                                                                                                                                                                      <  2:990959/219‑1 (MQ=255)
                      gCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAACAAACGGCGGTCGCTGGCTAACACTGATGTCGGGTAATCCTGc                                                                                                                                                                                                      >  1:767621/1‑238 (MQ=255)
                      gCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTTGCGAACACTGATGTCGGATAATCCTGc                                                                                                                                                                                                      >  1:294041/1‑238 (MQ=255)
                                       tGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAACGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGCACGTCCCTTGCTAACACGTAAGTCCGTTATTCCTGCCCCACTTTGCCATcaaca                                                                                                                                                                                    >  1:497940/1‑239 (MQ=255)
                                       tGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCCCCCCCGGCCCAAAACTGAAAAAACGGCGGGCCCCGGCCAAACCCTCTGTCGGGTAATCCTGCCACAAATTTCCacccaaa                                                                                                                                                                                    >  2:1072318/1‑233 (MQ=255)
                                             tcccACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAAcggg                                                                                                                                                                               <  1:745743/235‑1 (MQ=255)
                                              ctcACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGCCGGTCGCTTGCTTACACTGATGTCGGATAATCCTGCCCCAATTTGCCATCAACAAcggggc                                                                                                                                                                             >  1:1082218/1‑237 (MQ=255)
                                                         agggcgcAAGAATCGGCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACggg                                                                                                                                                                  <  1:790283/236‑1 (MQ=255)
                                                         aGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGCCGCTGGCTAACACTGATGTCGGATAATCCTGCCCCACTTTGCAATCAACAACGGGCGGGGTAAACcgg                                                                                                                                                                  >  1:950915/1‑239 (MQ=255)
                                                              gccaagatgctccccGGAACCCCAAAGGCGCTTTTTTCGCCGTTTTCGGCATCGATCCCTGGTGTAGTGAGGAACAGCTTGCCCGGACCAACAAAATACTGATCCGCGGCATATCCTCCAAAGCCGGCCCACTGCGCCCCCGGCTAAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                                                                              <  2:397981/225‑1 (MQ=255)
                                                              cccaggacgcgcccaggAACCCCAAAGGCCATTTTTGCGCCGTTTTCGGGATCGATCCCGGGTGTAGTGAGTAACAGCGTGCACGGACCAACAAAATCCTGACCCGTGGCAATCCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGGGGTCGCTGGCTAACACTGAGGTCGGATAACCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                                                                              <  2:766398/223‑1 (MQ=255)
                                                               ccAGAATGCTCGCCGGAACGCCAAAGTCGTTATTTTCCCCGGTTTCGGCATCGATCCCTGGTGTAGTGAGTAACAGCTTGCCCGGACCAACAAAATACTGACCCGCGGCATAGCCTTCAAAGCCGGGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAAc                                                                                                                                                            <  2:511701/239‑1 (MQ=255)
                                                                     tcgtcgCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGCACCGGGCCAACACCGAGGTCGGGTAAACCTTCCCCAATTTTCCCCCAACAACCGCCGGGAGAAACGGGGGGaaaaactga                                                                                                                                                      >  2:934242/1‑232 (MQ=255)
                                                                          gCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGAGAATCCTGCCCCAAATTGCCATCAACAACGGGCGGGGTAAACGGGCGGGAACGCTTAAAgc                                                                                                                                                  >  1:892711/1‑238 (MQ=255)
                                                                                     aaaggctcttgttggtgcgttttCGGTATCGATACCTGGTGGAGGGAGTAACAGCTTGAACGAAAAAAAAAAAGACTGTGCCGCGGGGTAACCTCCAAAGCGGGGCCAGTCCTCCCCCGTGTCAAAACAGAAAAACCGGCGGTGACCGGCTAACACTGATGGCGGATAATCCTGCCACAATTTGACATCAACAACGAGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                      <  2:340967/219‑1 (MQ=255)
                                                                                           gtttttttCGCCGGTTTCGGCAGTGGTCCCTGGTGTAGGGATTAACAGCTTGCCGGGATCAACAAAATACTGCTCCGCGGCATACCCGTCAACGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgc                                                                                                                                <  2:791281/233‑1 (MQ=255)
                                                                                             tataTTCGCCGGTTTCGGCATCGATACCTGGTGTAGGGAGTACCAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                              <  1:106632/239‑1 (MQ=255)
                                                                                             tagatTCGCCGGTTTCGGCATCGATCCCTGGTGTAGGGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                               <  1:641629/235‑1 (MQ=255)
                                                                                                     ccGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACAATGATGTCGGATAATCCTGGCCACATTTGCCATCCAACACGGGAGGGGTAAAAAGGCGGGACAACTCAAAGACCGCAAGaaaaaccacagtcgcttaca                                                                                                                       >  2:106632/1‑220 (MQ=255)
                                                                                                      ggttttCGGAGTCGTTTCCTGGGTTAGTGAGTAACGTGTTGCAGGGAACAACAAAATACTGGTCCGCGGCAAATCCTTAAAAGCCGGGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATc                                                                                                                     <  2:294041/236‑1 (MQ=255)
                                                                                                        gtttcggacacatttccTGGTTTGGTGGGTAACAGCTTGCCCGGATCAACAAAATCCGTCTCCGGGGAAATTCCTTCAAAGCCGGGCCCCTTCGCCCCCGGCTCAAAACAGAAAAACCGGCGGTCGATGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATccc                                                                                                                   <  2:813930/224‑1 (MQ=255)
                                                                                                           tCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAACTTACATCACGCAAAGAAAGCCTTTCGCGCTTCCAACCCTa                                                                                                                 >  1:908171/1‑238 (MQ=255)
                                                                                                                  cGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGACGCTGGCCAACACTGATGTCCGGAAATCCTGCCCCAAATTGCCCTCCAAAACGGGCGGGGTAAAAGGGCGGAACAACCTAACGCGCCCCAAAAAAACCTTTCGCGCTTCCATCCCAAttccacaa                                                                                                         >  2:819351/1‑232 (MQ=255)
                                                                                                                   gATGCCGGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGGTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacac                                                                                                       <  1:696236/239‑1 (MQ=255)
                                                                                                                      aCCTGGTGTAGTAAGTAACAGTTTGCACGGATCAACAAAATACTGGTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCACGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                    <  1:209816/239‑1 (MQ=255)
                                                                                                                             gtgttgatgacaaGCTTGCACGGACCAAAAAAATACTGAGCCGCGGCATATCCTTCAAAGCCGTTCCACTTCGCCCCCGGATCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTGGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                             <  2:246688/227‑1 (MQ=255)
                                                                                                                              tAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGAGCCGCGGATTATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTAAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                             <  1:230829/238‑1 (MQ=255)
                                                                                                                                 tGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGTGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTTCGCGCTTCAATCCCTATCTCCACACACCCGACCCACAg                                                                                          >  1:731461/1‑238 (MQ=255)
                                                                                                                                  gAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGGCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCCACACACTCAGCCCACAccc                                                                                        >  1:258966/1‑239 (MQ=255)
                                                                                                                                   aGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAAACCTGTTGTCGGATAATCCTGCCACAATTTGCCCTCAACAACCGGCGGGATAAAAGGGCGGCACAGCTTAAAGCGCGCAAGAATAGCCCTGCCGCCCTCAATCCCCATCTCAAAACACCCAACCcaccacc                                                                                        >  2:662358/1‑234 (MQ=255)
                                                                                                                                   aGTAACAGCTTGCACGGAAAAACAAAATCCTGACCCGCGGCATACCCTTCAAAGCCGGGCCCCTTCGCCCCCGGCTCAAAACGGAAAAAACGGCGGTCGCTGGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                 <  2:523852/214‑1 (MQ=255)
                                                                                                                                     tAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGCGGGGGTAAACGGGCGGAACAGCTTACCACGCGCACGAAAAGCCCTGTGCGCCTCAACCCCCACCTCAAACACACCCACCCCACACCCg                                                                                       >  2:725771/1‑238 (MQ=255)
                                                                                                                                     tAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                         <  1:1025641/219‑1 (MQ=255)
                                                                                                                                       aCAGCTTGCCCGGAACAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGTGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGc                                                                                   <  1:217448/239‑1 (MQ=255)
                                                                                                                                          gCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTCACACTGATGTCGGATAATCCTGCCCCAATTTGCCATCCACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCACGCAAAAAAAACCTTTCCGGCCTCAATCCCAAACTCCacacaccaaccccccaaccccccccc                                                                                 >  2:798445/1‑218 (MQ=255)
                                                                                                                                             tGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACACCCCGCCAGAAAAGCCTTGCGCCCTTCAACCCCTATCTCAacacacctgccccttataaacatccccga                                                                              >  1:523852/1‑215 (MQ=255)
                                                                                                                                               cACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCCACACACTCAGCCCCCAGCCGCCCCCCCcttc                                                                            >  1:974858/1‑236 (MQ=255)
                                                                                                                                                aCGGATCAACAAAATACTGATCCGCGGCATATCCCTCAAAGCCGTGCCACTTCGCCCCCGGCTAAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcc                                                                          <  1:387315/239‑1 (MQ=255)
                                                                                                                                                       aacaaAAAACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                   <  1:1072318/239‑1 (MQ=255)
                                                                                                                                                         caaAATACTGATCCGCGGGATATCCTTCAAAGCCGTGCCACTTCCCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACCCGGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGcc                                                                                  <  1:977797/222‑1 (MQ=255)
                                                                                                                                                              tACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGGTAAACGGGCGGAACAGCTTAAAGCGCGCAAGAAAAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACCCCCCCCCCCCCCCTCCTCAAtttt                                                           >  1:133068/1‑239 (MQ=255)
                                                                                                                                                              tACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGAAAATCCTGCCACAATTTGGCATCAACAACGGGCGGGATAAACAGGCGGAACAGCTTACAGCCCGCCAGAATAGCCCTGCGCGCTTCAATCCCTCACCCAACACACCCAACCCCCAACCCACACCCCCCCCCCCCCTCCTGaaaatt                                                            >  2:629831/1‑235 (MQ=255)
                                                                                                                                                                 tGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACCGATGTCGGGTAATCCTGCCACCATTTGCCCTCAACAACGGGCCGGCTAAACGGGCGGAACACCCTACAACCCGCAAGAACACCCTTTCACGCTCCAACCCCTCTCTCAACCCACTCACCACCCACCCCGCGCCCCCCCCCCCCCTCATCAAtttttgg                                                        >  2:374554/1‑236 (MQ=255)
                                                                                                                                                                  gctCCGCGGCATATCCTGCAAAGCGGGGCCCCTTCCCCCCCGGCTAAAAACGGAAAAAACGGGGGGCGATGGCTAAATCTGAGGGCGGATAAACCTGCCACAAGTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTGGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCg                                                       <  2:951100/237‑1 (MQ=255)
                                                                                                                                                                  gATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCCCAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAACTTACAGCGCGCAAAAAAAGCCCTGCGCGCTTCAATCCCTATCTCAACACACCCCGCCCCCACCCGCCCCCCCCTCCCCCCCTCACTAATTTTgcgg                                                       >  2:747028/1‑236 (MQ=255)
                                                                                                                                                                   aTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGCTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGGTAAACGGGCGGAAAAGCTTACAGCCCGCCAGAAAAGCCTTGCGCGCTTCAAACCCCATCTCAACACACCCACCCCCCAGCCCACCCCCCCCCCCCCCCTCATAAATTTTGGGGt                                                      >  2:543009/1‑239 (MQ=255)
                                                                                                                                                                   aTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAAAGGGCGGAAAAGCCTACAGCCCGCAAAAATAGCCTTGCGGGCTTCAATCCCATTTCTAACACCCACAGCCAACAACCCAccccccccccccccccctcccctttaaaaca                                                       >  2:977797/1‑222 (MQ=255)
                                                                                                                                                                   aTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTCGCCAAAACTTATGTCGGATAATCCTGCCCCAATTTGCCATCAACAACGCGCGGGGTAACCGGGCGGGACACACTACAACGCCCCAGAATAACCTTTCCGCCTTCAATCCCCACCTCAACACCACCAACCCACACCCGCCCCCCCTTCTcctttaaccaactcgaaccc                                                        >  2:949551/1‑220 (MQ=255)
                                                                                                                                                                     ccgccgctttcccccaaaaCCCGGGCCCCTCCCCCCCCGGACAAAAACTGAAAAAACGGCGGTCGCTGGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTa                                                    <  2:599872/223‑1 (MQ=255)
                                                                                                                                                                        cgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCACGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCCTGAATTTTTGCGTTAAc                                                  >  1:592863/1‑238 (MQ=255)
                                                                                                                                                                           caattCCTTCAAACCCGTGCCGCTTCGCCCCCGGCTCAAAACTAAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATc                                               <  1:819351/234‑1 (MQ=255)
                                                                                                                                                                           cATATCCCTCAAAGCCGTGCCACTCCCCCCCCGGCCAAAAACTGAAAAACGGGCGGGCGCTGGCTAACACTGATGTCGGAAAACCCTGCCACAATTTGCCATCAAAAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATc                                               <  2:1082218/238‑1 (MQ=255)
                                                                                                                                                                                ccTTCAAAGCCGTGCCACTTCGACCCCGGCTCAAAACTGAAAAAACGGCGGTCGGTGGCTAACACTGAGGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                        <  1:374554/239‑1 (MQ=255)
                                                                                                                                                                                 cTTCCAACCCGGCCCCCTTCCGCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGTCTAACCTTGATGTCGGAAAACCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgca                                       <  2:767621/239‑1 (MQ=255)
                                                                                                                                                                                 cTTCAAATCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgca                                       <  1:320414/239‑1 (MQ=255)
                                                                                                                                                                                          cgggCCACTTCGCCCCCGGCTAAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGGCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACa                                <  1:975320/234‑1 (MQ=255)
                                                                                                                                                                                             gCCACTTCGCCCCCGGCTCAAAACTGAAAAACCGGCGGTCGCTGGCTAACCCTGATGCCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                            <  1:158314/238‑1 (MQ=255)
                                                                                                                                                                                                    cGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTTACACTGATGTCGGATAATCCTGCCACAATTTGCCCTCAACAACGGGCGGGATAAACGGGCGGAACAGCCTAAAGCCCGCCAAGATAGCCTTGCGCGCATCCATCCCCTTCTCCACACACTCAGCCCACAGCCTACCCCCACCGTTCCCCTCATTAATTTTTCGCGTTACATACAGGTGCAGAAAACcgcgaaaaaaaa                     >  2:365524/1‑231 (MQ=255)
                                                                                                                                                                                                      ccccgGGCTAAAACTGAAAAAAACGGCGGCGGTGGGATAACCCGGAGGCCGGATAACCCTGCCAAAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAAACCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAggg                  <  2:133068/239‑1 (MQ=255)
                                                                                                                                                                                                      cccccGGTTCAAAACGAAAAAAACGGGGGTGCTGGGATAAACCGGATGGCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGATTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAggg                  <  2:1045789/239‑1 (MQ=255)
                                                                                                                                                                                                      cccccGGCTCAAAACTAAAAAACCGGCGGTCGCGGGCTAACCCGGAGTTCGGATAACCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAggg                  <  2:908171/239‑1 (MQ=255)
                                                                                                                                                                                                       ccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGc                 <  1:662358/239‑1 (MQ=255)
                                                                                                                                                                                                                  aaCTGAAAAACCGGGGCGCGCTGGCAAACACGGATGTGGGGTAACCCTGCCACAATTTGCCATAAACAACGGGCGGGAAAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa      <  2:974858/239‑1 (MQ=255)
                                                                                                                                                                                                                   accgcaaaaaaCGGCCGTCGCTGGTTAACCCGTTTGTAGGAAAGTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTTGCTCAACCCACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa      <  2:497940/234‑1 (MQ=255)
                                                                                                                                                                                                                      gAAAAAACGGCGGTCGCTGGCTAACCATGATGTCGGATAATCCTGCCACAATTTGCCATCAACCACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa  <  1:629831/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                         
AAACAGAATGGAGTTGAGATCGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGA  >  NC_000913/3107555‑3108006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: